mRNA_H-paniculata_contig2401.6498.1 (mRNA) Halopteris paniculata Hal_grac_a_UBK monoicous

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_H-paniculata_contig2401.6498.1
Unique NamemRNA_H-paniculata_contig2401.6498.1
TypemRNA
OrganismHalopteris paniculata Hal_grac_a_UBK monoicous (Halopteris paniculata Hal_grac_a_UBK monoicous)
Homology
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BLAST of mRNA_H-paniculata_contig2401.6498.1 vs. uniprot
Match: A0A6H5JGF9_9PHAE (Complex1_LYR_dom domain-containing protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JGF9_9PHAE)

HSP 1 Score: 106 bits (265), Expect = 1.640e-24
Identity = 59/88 (67.05%), Postives = 69/88 (78.41%), Query Frame = 1 
Query:  217 MSGRASGVQREVTHLYRRVLRAARLKDSESNSTSTSDLVKTEFREQATSVGRTDFRTIEHLIRAGNKKLKLLSMPGMRAAEALTVNHR 480
            MS R SGVQREV  LYR +LRAAR+KD    + ST++LV+ EFR QA SV RTDFRTIEHL+RAGNKKLKLL MPG++AA  +TV  R
Sbjct:    1 MSARLSGVQREVNKLYRLLLRAARVKDGGEWAGSTTELVRAEFRAQAESVARTDFRTIEHLLRAGNKKLKLLKMPGVKAAAGITVVRR 88
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BLAST of mRNA_H-paniculata_contig2401.6498.1 vs. uniprot
Match: D7FX23_ECTSI (Heat shock protein, putative n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FX23_ECTSI)

HSP 1 Score: 102 bits (255), Expect = 1.560e-22
Identity = 65/120 (54.17%), Postives = 80/120 (66.67%), Query Frame = 1 
Query:  142 FKTWTP*RRALNVAAETPQTEPTIDMS---GRASGVQ----REVTHLYRRVLRAARLKDSESNSTSTSDLVKTEFREQATSVGRTDFRTIEHLIRAGNKKLKLLSMPGMRAAEALTVNHR 480
             +T T  RRA   +A T    P I+      R+ G+Q    REV  LYR +LRAAR+KD    + ST++LV+ EFR QA SVGRTDFRTIEHL+RAGNKKLKLL MPG++AA  +TV  R
Sbjct:   15 LETLTSKRRAAERSALTAVLSPYINADRERSRSVGIQSPTKREVNQLYRLLLRAARVKDGGEWAGSTTELVRAEFRAQAESVGRTDFRTIEHLLRAGNKKLKLLKMPGVKAAAGITVARR 134
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BLAST of mRNA_H-paniculata_contig2401.6498.1 vs. uniprot
Match: I2CRH0_NANGC (Lyr family of fe s cluster biogenesis protein n=1 Tax=Nannochloropsis gaditana (strain CCMP526) TaxID=1093141 RepID=I2CRH0_NANGC)

HSP 1 Score: 78.6 bits (192), Expect = 2.420e-14
Identity = 45/81 (55.56%), Postives = 57/81 (70.37%), Query Frame = 1 
Query:  226 RASGVQREVTHLYRRVLRAARLKDSESNSTSTSDLVKTEFREQATSVGRTDFRTIEHLIRAGNKKLKLLSMPGMRAAEALT 468
            RASG+Q+EV  LYRRVLRAAR+KD+      T  L +T FRE ATSV R DF+ IE LIR G+K+LK L+MP +    ++T
Sbjct:    5 RASGLQKEVLSLYRRVLRAARVKDAGKIDGGTYTLARTRFREDATSVSRMDFQKIEFLIRQGHKQLKYLNMPSVLRGASVT 85
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BLAST of mRNA_H-paniculata_contig2401.6498.1 vs. uniprot
Match: A0A7S3Y3W0_HETAK (Hypothetical protein n=1 Tax=Heterosigma akashiwo TaxID=2829 RepID=A0A7S3Y3W0_HETAK)

HSP 1 Score: 72.4 bits (176), Expect = 8.060e-12
Identity = 42/83 (50.60%), Postives = 57/83 (68.67%), Query Frame = 1 
Query:  226 RASGVQREVTHLYRRVLRAARLKD--SESNSTSTSDLVKTEFREQATSVGRTDFRTIEHLIRAGNKKLKLLSMPGMRAAEALT 468
            R SG+Q+EV  LYR+ LR AR KD  S SN  S  +  ++EFREQA+ V ++DF+TIEH+IR G K++KL +MPG +     T
Sbjct:   34 RLSGLQQEVLALYRQCLRVARKKDLGSSSNVRSLYEYCRSEFREQASKVKKSDFQTIEHMIRHGKKQMKLAAMPGFQGMTHFT 116
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BLAST of mRNA_H-paniculata_contig2401.6498.1 vs. uniprot
Match: A0A7S1ZYE5_TRICV (Hypothetical protein n=1 Tax=Trieres chinensis TaxID=1514140 RepID=A0A7S1ZYE5_TRICV)

HSP 1 Score: 61.2 bits (147), Expect = 5.750e-8
Identity = 40/99 (40.40%), Postives = 54/99 (54.55%), Query Frame = 1 
Query:  226 RASGVQREVTHLYRRVLRAARLKDSESNST-------------------------STSDLVKTEFREQATSVGRTDFRTIEHLIRAGNKKLKLLSMPGM 447
            R SG+QR+V  LYR VLR A  KD  + S                          +T+   ++EFR++A S+ R DFRTIEH+IR G K++KLL MPG+
Sbjct:    6 RLSGLQRQVLALYRSVLREAARKDRSAASNRNDGGEYFLVLLSTDKCPSSPPGDATTTSYARSEFRKKAASIPRNDFRTIEHMIRWGQKQIKLLRMPGV 104
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BLAST of mRNA_H-paniculata_contig2401.6498.1 vs. uniprot
Match: A0A5D6XGN7_9STRA (Uncharacterized protein n=1 Tax=Pythium brassicum TaxID=1485010 RepID=A0A5D6XGN7_9STRA)

HSP 1 Score: 58.9 bits (141), Expect = 5.270e-7
Identity = 40/100 (40.00%), Postives = 55/100 (55.00%), Query Frame = 1 
Query:  232 SGVQREVTHLYRRVLRAARLKDSE------------------SNSTSTSDLVKTEFREQATSVGRTDFRTIEHLIRAGNKKLKLLSMPGMRAAEALTVNH 477
            SG+Q++V  LYRR L+AAR KD+E                  +NS +T   V+  FR+ A SV R DF  IEHL+R G + LK+L    M+AA    ++H
Sbjct:   28 SGLQKQVLTLYRRALQAARRKDAELAASAPPTTPLDPSTANPTNSNATLAFVRERFRDDAGSVRRMDFNVIEHLLRKGERDLKMLER--MKAANFTRISH 125
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BLAST of mRNA_H-paniculata_contig2401.6498.1 vs. uniprot
Match: A0A6U0URI4_9STRA (Hypothetical protein n=1 Tax=Pinguiococcus pyrenoidosus TaxID=172671 RepID=A0A6U0URI4_9STRA)

HSP 1 Score: 56.2 bits (134), Expect = 5.350e-6
Identity = 32/67 (47.76%), Postives = 47/67 (70.15%), Query Frame = 1 
Query:  232 SGVQREVTHLYRRVLRAARLKDSESNSTSTSDLVKTEFREQATSVGRTDFRTIEHLIRAGNKKLKLL 432
            SGVQ EV +LYR++LR A+ K  ++ +     +V+ +FRE A SV R++ R IEHL+R G KK++LL
Sbjct:   65 SGVQTEVLNLYRQLLRLAKAKGPDTYA-----MVRNKFREDARSVKRSEIRLIEHLVRQGKKKVRLL 126
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BLAST of mRNA_H-paniculata_contig2401.6498.1 vs. uniprot
Match: A0A0W0DRB0_CANGB (Succinate dehydrogenase assembly factor 1, mitochondrial n=2 Tax=Candida glabrata TaxID=5478 RepID=A0A0W0DRB0_CANGB)

HSP 1 Score: 53.9 bits (128), Expect = 8.480e-6
Identity = 33/78 (42.31%), Postives = 46/78 (58.97%), Query Frame = 1 
Query:  217 MSGRASGVQREVTHLYRRVLRAARLKDSESNSTSTSDLVKTEFREQATSVGRTDFRTIEHLIRAGNKKLKLLSMPGMR 450
            M+ + SG+QREV HLYR  +R A  K  E N       +  EF +    + R DF TIEHL+R GN++LK+ S P ++
Sbjct:    1 MARKLSGLQREVIHLYRSAVRVAHTKPVE-NQPHFLQFIHAEFSKYR-DLPRKDFTTIEHLLRVGNRRLKMYSQPELK 76
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BLAST of mRNA_H-paniculata_contig2401.6498.1 vs. uniprot
Match: A0A1G4KNI3_9SACH (LANO_0H21902g1_1 n=1 Tax=Lachancea nothofagi CBS 11611 TaxID=1266666 RepID=A0A1G4KNI3_9SACH)

HSP 1 Score: 52.0 bits (123), Expect = 4.050e-5
Identity = 32/73 (43.84%), Postives = 46/73 (63.01%), Query Frame = 1 
Query:  217 MSGRASGVQREVTHLYRRVLRAARLKDSESNSTSTSDLVKTEFREQATSVGRTDFRTIEHLIRAGNKKLKLLS 435
            M+ R SG+QREV  +YR  +RAA  K   +N  +    ++TEF +    + R DF TIEHL+R GN++LK+ S
Sbjct:    1 MAKRISGLQREVLQVYRHCIRAAHRKPL-TNQPNFVRYIRTEFSKYR-DISRKDFSTIEHLLRVGNRRLKMYS 71
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BLAST of mRNA_H-paniculata_contig2401.6498.1 vs. uniprot
Match: K3X5D9_GLOUD (Complex1_LYR_dom domain-containing protein n=1 Tax=Globisporangium ultimum (strain ATCC 200006 / CBS 805.95 / DAOM BR144) TaxID=431595 RepID=K3X5D9_GLOUD)

HSP 1 Score: 52.8 bits (125), Expect = 4.650e-5
Identity = 34/75 (45.33%), Postives = 46/75 (61.33%), Query Frame = 1 
Query:  232 SGVQREVTHLYRRVLRAARLKDSE-----SNST---STSDLVKTEFREQATSVGRTDFRTIEHLIRAGNKKLKLL 432
            SG+Q +V  LY++ LR A+ KD E     S +T   ST   V+  FR+ A SVGR DF  IEHLIR G + +K++
Sbjct:   20 SGLQMQVLALYKKALRTAQQKDRELVAASSGATAEVSTLTYVRERFRDDARSVGRMDFNVIEHLIRKGERDIKMM 94
The following BLAST results are available for this feature:
BLAST of mRNA_H-paniculata_contig2401.6498.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 12
Match NameE-valueIdentityDescription
A0A6H5JGF9_9PHAE1.640e-2467.05Complex1_LYR_dom domain-containing protein n=1 Tax... [more]
D7FX23_ECTSI1.560e-2254.17Heat shock protein, putative n=1 Tax=Ectocarpus si... [more]
I2CRH0_NANGC2.420e-1455.56Lyr family of fe s cluster biogenesis protein n=1 ... [more]
A0A7S3Y3W0_HETAK8.060e-1250.60Hypothetical protein n=1 Tax=Heterosigma akashiwo ... [more]
A0A7S1ZYE5_TRICV5.750e-840.40Hypothetical protein n=1 Tax=Trieres chinensis Tax... [more]
A0A5D6XGN7_9STRA5.270e-740.00Uncharacterized protein n=1 Tax=Pythium brassicum ... [more]
A0A6U0URI4_9STRA5.350e-647.76Hypothetical protein n=1 Tax=Pinguiococcus pyrenoi... [more]
A0A0W0DRB0_CANGB8.480e-642.31Succinate dehydrogenase assembly factor 1, mitocho... [more]
A0A1G4KNI3_9SACH4.050e-543.84LANO_0H21902g1_1 n=1 Tax=Lachancea nothofagi CBS 1... [more]
K3X5D9_GLOUD4.650e-545.33Complex1_LYR_dom domain-containing protein n=1 Tax... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
H-paniculata_contig2401contigH-paniculata_contig2401:3710..6373 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
OGS1.0 of Halopteris paniculata Hal_grac_a_UBK monoicous2021-02-24
Properties
Property NameValue
Taxonomic scopeViridiplantae
Seed ortholog score60.5
Seed ortholog evalue4.8e-07
Seed eggNOG ortholog3711.Bra017056.1-P
KEGG koko:K18167
Hectar predicted targeting categoryother localisation
EggNOG free text desc.Belongs to the complex I LYR family
EggNOG OGs2CJR6@1,2S3U6@2759,37W9A@33090,3GKAD@35493,3HURC@3699
Ec32 ortholog descriptionComplex 1 LYR protein
Ec32 orthologEc-10_003990.1
COG Functional cat.S
Best tax levelBrassicales
Best eggNOG OGNA|NA|NA
BRITEko00000,ko03029
Exons2
Model size1381
Cds size270
Stop1
Start1
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_H-paniculata_contig2401.6498.1prot_H-paniculata_contig2401.6498.1Halopteris paniculata Hal_grac_a_UBK monoicouspolypeptideH-paniculata_contig2401 3926..5478 +


The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622929689.286723-UTR-H-paniculata_contig2401:3709..39251622929689.286723-UTR-H-paniculata_contig2401:3709..3925Halopteris paniculata Hal_grac_a_UBK monoicousUTRH-paniculata_contig2401 3710..3925 +
1691480968.2242184-UTR-H-paniculata_contig2401:3709..39251691480968.2242184-UTR-H-paniculata_contig2401:3709..3925Halopteris paniculata Hal_grac_a_UBK monoicousUTRH-paniculata_contig2401 3710..3925 +
1622929689.3310816-UTR-H-paniculata_contig2401:5478..63731622929689.3310816-UTR-H-paniculata_contig2401:5478..6373Halopteris paniculata Hal_grac_a_UBK monoicousUTRH-paniculata_contig2401 5479..6373 +
1691480968.253026-UTR-H-paniculata_contig2401:5478..63731691480968.253026-UTR-H-paniculata_contig2401:5478..6373Halopteris paniculata Hal_grac_a_UBK monoicousUTRH-paniculata_contig2401 5479..6373 +


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622929689.3026764-CDS-H-paniculata_contig2401:3925..40631622929689.3026764-CDS-H-paniculata_contig2401:3925..4063Halopteris paniculata Hal_grac_a_UBK monoicousCDSH-paniculata_contig2401 3926..4063 +
1691480968.2353897-CDS-H-paniculata_contig2401:3925..40631691480968.2353897-CDS-H-paniculata_contig2401:3925..4063Halopteris paniculata Hal_grac_a_UBK monoicousCDSH-paniculata_contig2401 3926..4063 +
1622929689.3156748-CDS-H-paniculata_contig2401:5346..54781622929689.3156748-CDS-H-paniculata_contig2401:5346..5478Halopteris paniculata Hal_grac_a_UBK monoicousCDSH-paniculata_contig2401 5347..5478 +
1691480968.2443688-CDS-H-paniculata_contig2401:5346..54781691480968.2443688-CDS-H-paniculata_contig2401:5346..5478Halopteris paniculata Hal_grac_a_UBK monoicousCDSH-paniculata_contig2401 5347..5478 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_H-paniculata_contig2401.6498.1

>prot_H-paniculata_contig2401.6498.1 ID=prot_H-paniculata_contig2401.6498.1|Name=mRNA_H-paniculata_contig2401.6498.1|organism=Halopteris paniculata Hal_grac_a_UBK monoicous|type=polypeptide|length=90bp
MSGRASGVQREVTHLYRRVLRAARLKDSESNSTSTSDLVKTEFREQATSV
GRTDFRTIEHLIRAGNKKLKLLSMPGMRAAEALTVNHRR*
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mRNA from alignment at H-paniculata_contig2401:3710..6373+

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_H-paniculata_contig2401.6498.1 ID=mRNA_H-paniculata_contig2401.6498.1|Name=mRNA_H-paniculata_contig2401.6498.1|organism=Halopteris paniculata Hal_grac_a_UBK monoicous|type=mRNA|length=2664bp|location=Sequence derived from alignment at H-paniculata_contig2401:3710..6373+ (Halopteris paniculata Hal_grac_a_UBK monoicous)
GCTCGCCGCAACTAGTGGTATGATGGTGATAACACAACGTGGAGAAAGAC
GTCGTTTTTGCTGTTTCATGCTGGGTCCAACTCTGCAGTCTATTGTCCTC
CACAACAGCTTCTTAACGCACAAGGTGTGAGAACGACAGGTTTCAAAACT
TGGACACCTTAAAGACGTGCCTTGAACGTTGCAGCAGAAACACCGCAAAC
AGAGCCCACCATCGACATGTCAGGAAGGGCATCAGGAGTACAAAGGGAAG
TGACCCATTTATATCGCCGAGTGCTTCGAGCAGCAAGACTGAAGGACAGC
GAGAGCAATAGCACAAGTACTTCAGATTTGGTGAAGACGGAGTTTCGTGA
GCAGGTGCATCCTCGGACTTATTTTTAGTACATGAGGAAAACGACGCGAT
CTACGAGCATTTTTTACTACAATAAGAGTATATTTGAATATACACTCGAC
CAGTGATCATCTCTCATCGATGCGATCAAGTTCGGCGTTTGGCAGTTTGA
AACAGCACTGATAGAGTATGACATGCCTATATTTTTATGTGGGCCAAGCT
GCTGTAACCACGCTCGTCCAGCGATCTGTCGGCGGTTCTTTCAATCAACT
CCGAGCGAGCGTTGCTCGAGGTCGATACCTGTCCAACTCTCGGCAATCCA
TTCAAAATGAAACATACTGTGCTGTACTAACACATATGCGAAGTACTACG
ATATGATCATGTTATTCCCCTAAACTCCAACCCTAAGTTAAGGCGGATTT
ACCTCACCTCACATTCGCCTCTTCGTGGTATAAATACACTCTGGGTCCGG
TAGCGTTTAGTTCGGCCCAAACGCTGCGCCGCTCGGCGCCGCACAGACCA
GCCCAAGTACCACGACCAACCCGAAAGGTCATCGCAGGATTGTCAGACTC
ATCGCAATGAATCAAACAGGCGTGAACGATGGAATTTGGCGACCAATATT
GTAAGCTCTATACTGGCTGGTCGGTTCAGTAGTGCCCACTCTAACACTTA
CCTCGTACTGCCACTGGGTTTAACGTTAACGATAGAGGTGGTTACTCCAC
GTTTTGTTGAACCAGATATTCCACCGACGGGCTTGAAGCTGGTCGGTTAA
AACATTGTGCAGTTTAGTCATAATTGCTGCTGAAGTTCGAATTGAAATGC
ACACGTTTCGATTGGATTGTGTGAATGAGACTCGAAATCCGAATTCGCTT
AACGTGTCGGGTGCGAACGAGCCGCCACCTCGCTAATTGTCCAAGTGTTC
CTAGCCCATTACAACAACAGACGCGGTCGTGGTCTAGCGAAGTAGGATTG
CATCCAACGAGAATGTGGGATTTAGCACCACGATCACTCTGACCGTTTAA
GCCTACCACCCCCCGAATTTTGATCTGCGGACAGCTTGCGCCAACAAATG
AAAGCACGTCACGGTTCCGTCAGAACTGCAATGTACAAGCGCTCTTGCGA
ATTTAGTTCCGGCCACTGCCCGACTGGGTTTCCAGATCACATCAGTCCGC
AATGTTTTATTTCATGTTATATCTGCTTGGGCTTTCCTCGTGTTAATCAG
GCCATTCGCGGCATATTTTTACCCCCTCCACTTCCCGCCCTGCTTCCATG
CCTCGCTGACATGGCATTTATAATATTGATGAGACAGGCTACGTCGGTAG
GGCGAACGGATTTCCGCACAATAGAGCACCTTATCCGCGCCGGCAACAAG
AAGCTCAAACTGTTGAGTATGCCAGGGATGCGAGCGGCGGAAGCACTCAC
AGTCAATCACCGACGATGACACGTTGTCCCCCACGTCCTCCAGGCGGTGG
GCAGAGGTGTGTTGGATATAGGGCCGCGTTTGGGCCAAGGGCGGTACCTT
ACGCAATTTGTTGACAACAACGTAGTGTTGTGCTGAATGCGCACCCAGTA
TGATCTGTTATAAAGGTACGTATGCGACGTACTGCACTGTACAGTGTGGT
CCCAGTTGTGTCGAGGAGGATCCACAGAAACGGACTCTTTGTCAAAACCG
TTAGGCAAACGCAGCTAAATGTGCTTGCAGCTTGTCGTCGTTTTTCTTGC
GTGTTATTCGTGGTGCAGTTGGTCGAAATGGGCTAGCGGGTGGAGCTGGG
AGTTGAAGCGTAGTGGCTGTACGCTGTCGTAATTCATAGTATGTTGGAAC
GTGTCGGCACATGTTCCATTTCTGCTGAAGCAAGAAGTATTTGGATTATG
CAGGTTCAGCTCGGAATAGGGACTTGTGAGTTGGACGTATCAATATTTTG
CTGGCTGCCATAAAACGTGGGGCTGTATACGTAGCTCAAGGTGTGAGCAT
TGGCCAAGATAAAGATTGGGAACGCGGAGCTAGTGCAGAGATGAGGCAGC
GGAGTGAATGTAGTGACCGCGTAATACACGGGCTGACCTTTCATGCGATA
CTCAATTGCAGCCGCTCCGCATCATCGTAGCCCTCATTGGTATGTATTTT
TTTTTTCTCTCACAACGTTTTAATAGAGAGGTACTCTTACTCTTCTAGAG
GACTTGGATTGTACCAGCATCTGTACATGTAAGCTGCATTTAGGACGAGT
ATGAATATGGGGTTATCATAACACGAGACACGCGTCGGTATATGCCATGC
GAGTAGTCGCCGAAACTATTGGTACAAGTAGCTGCTGTACAAATACGAGG
AATTCCATAACAAA
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Coding sequence (CDS) from alignment at H-paniculata_contig2401:3710..6373+

>mRNA_H-paniculata_contig2401.6498.1 ID=mRNA_H-paniculata_contig2401.6498.1|Name=mRNA_H-paniculata_contig2401.6498.1|organism=Halopteris paniculata Hal_grac_a_UBK monoicous|type=CDS|length=540bp|location=Sequence derived from alignment at H-paniculata_contig2401:3710..6373+ (Halopteris paniculata Hal_grac_a_UBK monoicous)
ATGTCAGGAAGGGCATCAGGAGTACAAAGGGAAGTGACCCATTTATATCG
CCGAGTGCTTCGAGCAGCAAGACTGAAGGACAGCGAGAGCAATAGCACAA
GTACTTCAGATTTGGTGAAGACGGAGTTTCGTGAGCAGATGTCAGGAAGG
GCATCAGGAGTACAAAGGGAAGTGACCCATTTATATCGCCGAGTGCTTCG
AGCAGCAAGACTGAAGGACAGCGAGAGCAATAGCACAAGTACTTCAGATT
TGGTGAAGACGGAGTTTCGTGAGCAGGCTACGTCGGTAGGGCGAACGGAT
TTCCGCACAATAGAGCACCTTATCCGCGCCGGCAACAAGAAGCTCAAACT
GTTGAGTATGCCAGGGATGCGAGCGGCGGAAGCACTCACAGTCAATCACC
GACGATGAGCTACGTCGGTAGGGCGAACGGATTTCCGCACAATAGAGCAC
CTTATCCGCGCCGGCAACAAGAAGCTCAAACTGTTGAGTATGCCAGGGAT
GCGAGCGGCGGAAGCACTCACAGTCAATCACCGACGATGA
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