Ggra9175.t1 (polypeptide) Gracilaria gracilis GNS1m male

BLAST of Ggra9175.t1 vs. uniprot
Match: A0A2V3IPA9 (DNA topoisomerase n=1 Tax=Gracilariopsis chorda TaxID=448386 RepID=A0A2V3IPA9_9FLOR)

HSP 1 Score: 1059 bits (2739), Expect = 0.000e+0
Identity = 507/630 (80.48%), Postives = 569/630 (90.32%), Query Frame = 0

Query:    1 MALRILNVAEKPSAAKEIMNVLKGGQPAQSRHGRSRYNPIYEFNMSLQGRPSLMIFTSVTGHIKNTDFEDRYRKWGSCDPSQLLVPSQTRVEWYVSEERRPLAETLRTEARRADWLILWLDCDSEGERIAVDVADICKEAKRSLLVKRARFSAMTRGDLFRAVNQLDVPNERVARMVATRQEIDLRAGSAYTRFLTKQLEKYALTDMDKQVISYGPCQFPTLGLVVDRWLRIQNFVSRPFWVFELVLKDCRVPLEWFRKQLFDEYSAMTLYELCAEEAQADGNVATVKRVDKRVKTRWRPLPLSTVELQKVAARSLRINSHRTMEIAEALYNKGLISYPRTETNRFSRTYDLKGLIQKHVNHTTWGSFAERLLSPSTNQDPLTFLWPRSGPQDDGAHPPIHPTQGAPLSFDSTDHARVYQYITKRFLATCSIDAVGSETRTDVLVGSAEYFTAKGLIVEQKGYLEVLYPFEQWTDKDMPLSLLEIDACIPIESFTFRRSQTQPPPLLQEADLIALMDHHGIGTDATIAEHIRKVQDRRYVEKLAQGRFSPTEIGKALVIAHEQCQIHLARPHMRAKQENELKRILNGEIEAAQVLQSALEEYRAKLSYLKTRRAVLHTVFSRHFSEVVST 630
            M LRILNVAEKPSAAKEI  VLKG Q A SR G+SRYNPIYEFNMS+QG  SLM+FTSVTGHIK+TDFE RYRKWGSCDPS+LL+P+QT VEW+++E+++PLA TLR EARRADWL+LWLDCDSEGERIA DVADICKE K +++VKRARFSAMTRGDL RA+NQL +P+ERVA+MVATRQE+DLRAGSAYTR+LTKQLEKY+LTD + Q+ISYGPCQFPTLGLVVDRWL IQNFVSRPFWVFEL L+DC VPLEWFRKQLFDEY+AM LYELCAEEA+ADGNV TVKR DKR+KTRWRPLPLSTVELQKV++RSLRINSHRTMEIAE+LYNKGLISYPRTETNRFSRTYDLK  IQK   H TWG FAERLLSP  + DP+TF WPR+GP DDGAHPPIHPT  AP  FDS DH RVY+YI KRFLA+CSIDAVGSETR+DVLVGSAE FTA+GLIVEQKGYLEVL+P+E+W DK+MP+SLL++++ +P ESFTFRRSQT PPPLLQEADLIALMDHHGIGTDATIAEHIRKV DR YVEK+ QGRFSPTEIGKALVIAHEQC+IHLARPHMRAKQENELKRIL+GE++ ++VL SAL+EYR KL YLK RR VLH+VFS+HF++V  T
Sbjct:    1 MVLRILNVAEKPSAAKEITGVLKGNQAATSRQGQSRYNPIYEFNMSVQGHSSLMVFTSVTGHIKSTDFEARYRKWGSCDPSELLIPTQTAVEWFITEDKQPLARTLRAEARRADWLVLWLDCDSEGERIAWDVADICKEVKPTIVVKRARFSAMTRGDLLRAINQLTIPDERVAQMVATRQELDLRAGSAYTRYLTKQLEKYSLTDTENQIISYGPCQFPTLGLVVDRWLTIQNFVSRPFWVFELKLRDCEVPLEWFRKQLFDEYTAMALYELCAEEAEADGNVVTVKRADKRIKTRWRPLPLSTVELQKVSSRSLRINSHRTMEIAESLYNKGLISYPRTETNRFSRTYDLKDFIQKQTGHPTWGQFAERLLSPPNHNDPVTFNWPRTGPHDDGAHPPIHPTHEAPPVFDSPDHQRVYEYIAKRFLASCSIDAVGSETRSDVLVGSAEIFTARGLIVEQKGYLEVLHPYEKWNDKEMPISLLDVNSQLPFESFTFRRSQTIPPPLLQEADLIALMDHHGIGTDATIAEHIRKVLDREYVEKVMQGRFSPTEIGKALVIAHEQCEIHLARPHMRAKQENELKRILSGEVQPSEVLCSALDEYRTKLDYLKNRRNVLHSVFSQHFAQVTPT 630          

BLAST of Ggra9175.t1 vs. uniprot
Match: R7Q6W3 (DNA topoisomerase n=1 Tax=Chondrus crispus TaxID=2769 RepID=R7Q6W3_CHOCR)

HSP 1 Score: 912 bits (2356), Expect = 0.000e+0
Identity = 435/630 (69.05%), Postives = 526/630 (83.49%), Query Frame = 0

Query:    1 MALRILNVAEKPSAAKEIMNVLKGGQPAQSRHGRSRYNPIYEFNMSLQGRPSLMIFTSVTGHIKNTDFEDRYRKWGSCDPSQLLVPSQTRVEWYVSEERRPLAETLRTEARRADWLILWLDCDSEGERIAVDVADICKEAKRSLLVKRARFSAMTRGDLFRAVNQLDVPNERVARMVATRQEIDLRAGSAYTRFLTKQLEKYALT-DMDKQVISYGPCQFPTLGLVVDRWLRIQNFVSRPFWVFELVLKDCRVPLEWFRKQLFDEYSAMTLYELCAEEAQADGNVATVKRVDKRVKTRWRPLPLSTVELQKVAARSLRINSHRTMEIAEALYNKGLISYPRTETNRFSRTYDLKGLIQKHVNHTTWGSFAERLLSPSTNQDPLTFLWPRSGPQDDGAHPPIHPTQGAPLSFDSTDHARVYQYITKRFLATCSIDAVGSETRTDVLVGSAEYFTAKGLIVEQKGYLEVLYPFEQWTDKDMPLSLLEIDACIPIESFTFRRSQTQPPPLLQEADLIALMDHHGIGTDATIAEHIRKVQDRRYVEKLAQGRFSPTEIGKALVIAHEQCQIHLARPHMRAKQENELKRILNGEIEAAQVLQSALEEYRAKLSYLKTRRAVLHTVFSRHFSEVVS 629
            M LR+LNVAEKPSAAKEI NVLK GQ  ++R G SR+N IY+F+M+L G  + M FTSV GH+K TDFEDR RKWGSCDP  LL  S+T+VEW+V E+++P+AETLR E+RRADWL+LWLDCDSEGE+IA DVAD+CK+ K S++V+RARFSAMT GDLFRAV QL  PNERVA+MV+TRQEIDLRAGSAYTRFLT QLEK++L  D ++Q++SYGPCQFPTLGLVVDRWL+IQNFV RPFWVF+L +KDC+V  +W RK LFDEY++M LYELC EEA+ DG + +V RVDKR+K RWRPLPLSTVELQKVA+RSLRI+SHR ME+AE+LYNKGLISYPRTET+RF ++Y+L+ L+ K V H  WG FA RLLSP + +D L F WPRSG  DDGAHPPIHPT  AP  F+STDH ++Y+YITKRFLA CSI+A  SETR +V  G++E+FTA+GLI+E+KGYLEV+YPFE+WTDKDMP++LL ++A + I+S   R+SQTQPPPLLQE+DLIALMDHHGIGTDATIAEHI+KV DR YVEK+  G FSPT+IG+ALVIAHEQCQ+HLARP MRAKQE +LK+IL  E+E A VL +AL EY++K  +L+  R VL TVFS  F  V +
Sbjct:    1 MVLRVLNVAEKPSAAKEIANVLKAGQQCETRPGLSRFNQIYDFDMNLAGNRARMSFTSVLGHLKGTDFEDRVRKWGSCDPVDLLDRSRTKVEWFVPEDKQPIAETLRRESRRADWLVLWLDCDSEGEKIAFDVADVCKQGKPSIVVRRARFSAMTHGDLFRAVAQLGEPNERVAQMVSTRQEIDLRAGSAYTRFLTIQLEKFSLVGDDERQIVSYGPCQFPTLGLVVDRWLKIQNFVRRPFWVFDLFVKDCKVQFDWARKHLFDEYTSMALYELCIEEAEQDGMMVSVTRVDKRMKNRWRPLPLSTVELQKVASRSLRISSHRAMEVAESLYNKGLISYPRTETDRFDKSYNLRDLVGKQVGHLDWGPFASRLLSPPSEEDALAFTWPRSGSNDDGAHPPIHPTDVAPARFESTDHQKIYEYITKRFLAACSIEARASETRVEVRAGTSEFFTARGLIIEEKGYLEVMYPFEKWTDKDMPVALLHVNAKLLIDSLMLRQSQTQPPPLLQESDLIALMDHHGIGTDATIAEHIKKVLDRSYVEKVRGGGFSPTQIGQALVIAHEQCQLHLARPDMRAKQERQLKQILTSEMEPADVLTNALSEYQSKFRHLRQNRDVLDTVFSERFQAVTA 630          

BLAST of Ggra9175.t1 vs. uniprot
Match: DNA topoisomerase 3-like n=1 Tax=Mercenaria mercenaria TaxID=6596 RepID=UPI001E1D5585 (DNA topoisomerase 3-like n=1 Tax=Mercenaria mercenaria TaxID=6596 RepID=UPI001E1D5585)

HSP 1 Score: 693 bits (1789), Expect = 4.620e-239
Identity = 347/576 (60.24%), Postives = 428/576 (74.31%), Query Frame = 0

Query:   54 MIFTSVTGHIKNTDFEDRYRKWGSCDPSQLLVPSQTRVEWYVSEERRPLAETLRTEARRADWLILWLDCDSEGERIAVDVADICKEAKRSLLVKRARFSAMTRGDLFRAVNQLDVPNERVARMVATRQEIDLRAGSAYTRFLTKQLEKYALTDM--DKQVISYGPCQFPTLGLVVDRWLRIQNFVSRPFWVFELVLKDCRVPLEWFRKQLFDEYSAMTLYELCAEEAQADGNVATVKRVDKRVKTRWRPLPLSTVELQKVAARSLRINSHRTMEIAEALYNKGLISYPRTETNRFSRTYDLKGLIQKHVNHTTWGSFAERLLSPSTNQDPLTFLWPRSGPQDDGAHPPIHPTQGAPLSFDSTDHARVYQYITKRFLATCSIDAVGSETRTDVLVGSAEYFTAKGLIVEQKGYLEVLYPFEQWTDKDMPLSLLEIDACIPIESFTFRRSQTQPPPLLQEADLIALMDHHGIGTDATIAEHIRKVQDRRYVEKLAQGRFSPTEIGKALVIAHEQCQIHLARPHMRAKQENELKRILNGEIEAAQVLQSALEEYRAKLSYLKTRRAVLHTVFSRHFSEV 627
            ++FTSVTGH+K++DFEDR+RKWGSCDPS LL P+ ++V W+V EE+R + + LR EARRA  L+LWLDCDSEGE+IA DVAD+CK A R+L+VKRARFSAMT  +L  A++ L   + R+  MVATRQEIDLRAG+AYTRFLT+ + K+ ++    D ++ISYGPCQFPTLGLVVDRWL I++FV R FWV +L L+ C V  EW R +LFD+YSA TLYELC ++  A GN A VK V KR + R+RP+PLSTVELQK A+R LRI+S RTME AE LY  GLISYPRTET+ FS +Y+L+ LI  H ++ TWGSFA RLL+P+T  DPLTF WPRSG  DD AHPPIHPT G P  F   D  RV+ YIT+RFLA+CSIDA G+ET+  V VG AE F A+GLIVE +GYLE++ P+E+W D+ MP  LL   A + IE  T R SQT PPPLL EADLIALMD HGIGTDATIAEHI+KVQDR YV KL+  RF P+++G ALV A E+CQI LARP MR+ QE   K+I  GE +AA V   AL ++ +    L++   +L   F+  F  V
Sbjct:    1 LVFTSVTGHLKSSDFEDRHRKWGSCDPSVLLDPTCSQVTWFVPEEKRTVEQNLRQEARRAHTLVLWLDCDSEGEKIAQDVADVCKSANRNLVVKRARFSAMTGPELMHALHNLVNLDARMVAMVATRQEIDLRAGAAYTRFLTQVVGKFEISSDPDDSRLISYGPCQFPTLGLVVDRWLTIEHFVRRLFWVIDLKLRACDVQFEWARTRLFDQYSAHTLYELCVDDCAALGNQAQVKHVTKRTRDRYRPMPLSTVELQKAASRVLRISSDRTMEAAEKLYTAGLISYPRTETDSFSSSYELQSLISIHASNPTWGSFASRLLTPATTDDPLTFQWPRSGSNDDHAHPPIHPTGGEPPCFPDDDQRRVFAYITRRFLASCSIDARGAETQVQVRVGRAEVFGARGLIVEIQGYLEIMQPYERWADRSMPHELLNQGANVGIEQLTLRESQTSPPPLLAEADLIALMDRHGIGTDATIAEHIKKVQDRNYVRKLSDSRFEPSQLGIALVEALERCQIQLARPGMRSAQEISFKKIPTGERDAAHVKAEALSQFSSTFHRLRSCSQILDAAFAIRFQVV 576          

BLAST of Ggra9175.t1 vs. uniprot
Match: A0A1X6NQS1 (DNA topoisomerase n=1 Tax=Porphyra umbilicalis TaxID=2786 RepID=A0A1X6NQS1_PORUM)

HSP 1 Score: 592 bits (1527), Expect = 6.070e-195
Identity = 323/659 (49.01%), Postives = 430/659 (65.25%), Query Frame = 0

Query:    4 RILNVAEKPSAAKEIMN-VLKGG-QPAQSRHGRSRYNPIYEFNM-----SLQGRPS---LMIFTSVTGHIKNTDFEDRYRKWGSCDPSQLLVPSQTRVEWYVSEERRPLAETLRTEARRADWLILWLDCDSEGERIAVDVADICKEAKRSLLVKRARFSAMTRGDLFRAVNQLDVPNERVARMVATRQEIDLRAGSAYTRFLTKQLEKYALTD-----MDKQVISYGPCQFPTLGLVVDRWLRIQNFVSRPFWVFELVLKDCR-----VPLEWFRKQLFDEYSAMTLYELCAEEAQADGNV----ATVKRVDKRVKTRWRPLPLSTVELQKVAARSLRINSHRTMEIAEALYNKGLISYPRTETNRFSRTYDLKGLIQKHVNHTTWGSFAERLLS-----------PSTNQDPLTFLWPRSGPQDDGAHPPIHPTQGAPLSFDSTDHARVYQYITKRFLATCSIDAVGSETRTDVLVGSAEYFTAKGLIVEQKGYLEVLYPFEQWTDKDMPLSLLEIDACIPIESFTFRRSQTQPPPLLQEADLIALMDHHGIGTDATIAEHIRKVQDRRYVEKL--AQGRFSPTEIGKALVIAHEQCQIHLARPHMRAKQENELKRILNGEIEAAQVLQSALEEYRAKLSYLKTRRAVLHTVFSRHFS 625
            R+L VAEKPSAA+++++ V +GG   A+ R G+S+YN ++EF       S+   P+    ++FTSVTGH+   +FE RYR WGSCDPS LL  +Q+ +E    EE+ PL  TL+ EAR    L+LWLD DSEGE+IA DVA + ++ + ++ V+RARFSA+T  ++   +  LDV N RV  MVA RQEIDLR+G+A+TR LT+++ K   +D      D +V+SYGPCQFPTLGLVVDR+L I+NF+  PF+V +L L         VP  W R ++FD+Y A  LYE C E+A ++       A V  V K  + RW+PLPL+TVELQK A+R L ++SH  MEIAE LY  G ISYPRTET++FSR+ DL+ L+ +  +H  WG FA RLL+           P    D + F WPR GP DD AHPPIHPT+ AP    S + ARVY+Y+ +RFLA+CS+DA+G+ET  +  +G  E FTAKGLIVE +G+LEV +P+++W D +MP    ++     + S   R S T+PPPLL E+DLI+LMD HGIGTDATIAEH++KVQDR YV KL  A GRF P  +G ALV   E   +HLARP  RA+QE  +KRI +GE+  A ++  AL  Y A    L+T  A +    S H S
Sbjct:    5 RVLCVAEKPSAARQLVDAVCQGGVNSARRRPGQSQYNHVFEFETAAPIASISPSPTERVTLVFTSVTGHLFEQEFEARYRSWGSCDPSLLLDRTQSAIEVGPPEEKMPLVRTLQDEARNCMALVLWLDGDSEGEKIAADVASVVRQTRPTINVRRARFSAITTAEILHTLAHLDVLNTRVVEMVAARQEIDLRSGAAFTRLLTRRI-KNRFSDNRADASDAKVVSYGPCQFPTLGLVVDRFLTIRNFIRVPFFVIDLGLAAVAPATGVVPFSWARGRVFDQYVASVLYEACVEDAVSESPDGVPRAVVTGVRKNQRRRWKPLPLATVELQKEASRKLHLSSHLVMEIAEKLYTSGFISYPRTETDKFSRSIDLRALVAEQSSHGEWGGFAARLLANENRPGLGPGPPPDGYDIVQFDWPRDGPHDDAAHPPIHPTKVAPHGSLSGNEARVYEYVARRFLASCSVDAIGAETVVNARIG-VEAFTAKGLIVEHRGFLEV-FPYQRWADMEMPA--YQVGHPAAVRSLLLRESSTEPPPLLSESDLISLMDRHGIGTDATIAEHVKKVQDRNYVRKLPGATGRFEPLPLGLALVDGCEAVAVHLARPTERARQEAAMKRIASGEVAQAPMIVDALNAYTALFESLRTHAAEMEAAISNHLS 658          

BLAST of Ggra9175.t1 vs. uniprot
Match: A0A5J4Z1W2 (DNA topoisomerase n=1 Tax=Porphyridium purpureum TaxID=35688 RepID=A0A5J4Z1W2_PORPP)

HSP 1 Score: 559 bits (1441), Expect = 8.220e-183
Identity = 311/639 (48.67%), Postives = 415/639 (64.95%), Query Frame = 0

Query:    4 RILNVAEKPSAAKEIMNVLKGGQPAQSRHGRSRYNPIYEFNMSLQGRPSLMIFTSVTGHIKNTDFEDRYRKWGSCDPSQLLVPSQTRVEWYVSEERRPLAETLRTEARRADWLILWLDCDSEGERIAVDVADICKEAKRSLLVKRARFSAMTRGDLFRAVNQLDVPNERVARMVATRQEIDLRAGSAYTRFLTKQL-------EKYALTDMDKQVISYGPCQFPTLGLVVDRWLRIQNFVSRPFWVFELVLKDCR-VPL--EWFRKQLFDEYSAMTLYELCAEEAQADGNVATVKRVDKRVKTRWRPLPLSTVELQKVAARSLRINSHRTMEIAEALYNKGLISYPRTETNRFSRTYDLKGLIQKHVNHTTWGSFAERLLSPSTNQDP------LTFLWPRSGPQDDGAHPPIHPTQGAPLS--FDSTDHARVYQYITKRFLATCSIDAVGSETRTDVLVGSAEYFTAKGLIVEQKGYLEVLYPFEQWTDKDMPLSLLEIDACIPIESFTFRRSQTQPPPLLQEADLIALMDHHGIGTDATIAEHIRKVQDRRYVEKLAQGRFSPTEIGKALVIAHEQCQIHLARPHMRAKQENELKRILNGEIEAAQVLQSALEEYRAKLSYLKTRRAVLHTVFSRHF 624
            R+L VAEKPSAA+EI  VL GG    +R+  S+YN I EF+M+ Q +   ++FTSV+GHIK  DFE + + W S DP+ LL    +RV   VS ++ PL  TL+ EAR    LILWLDCDSEGE+IA +V +I K     + VKRARFSA+TR DLF A+  L +PNERVA MV+ RQEIDLRAG+A+TRFLT  L       +  + +   KQ ISYGPCQFP LGLVVDR+L I+NF S+ F+V +L+LK    +PL  EW R++LFD+++A   + LC E A      A V R  +  + RW+PLPLSTVE+Q  A+R L++ S + M  AEALY  G ISYPRTET+ F+   DL+ ++ +      W  F  +LL      +       + F  PR+G  DD AHPPIHP + AP S  F      R+++YIT+RFLA+CS+DAVGSET  +V +G  E F+ K LIVE+KG+ EV + +E+W ++D+P  L E D  + I+    R S+T+PPPLL E+DLI+LMD HGIGTDATIAEH++K+Q+R YV K AQ RF P  +G  LV   E C++ LARP +RA+QE ELKR+  GE+    +L  A++ YR   + L  RR+   + F  +F
Sbjct:    5 RVLCVAEKPSAAREISMVLAGGSKVVTRNSFSKYNRIMEFDMT-QPQEMKIVFTSVSGHIKEVDFEPKCQSWQSYDPALLLNREASRVISRVSADKEPLERTLKHEARHCQVLILWLDCDSEGEKIAFEVMEIVKSGNPRIDVKRARFSAITRQDLFHALCHLQLPNERVADMVSARQEIDLRAGAAFTRFLTLLLTERFGGVQAVSRSTGKKQPISYGPCQFPALGLVVDRFLMIKNFKSQAFYVIDLLLKTRNGIPLKFEWARQRLFDQHAAQVCFLLCHEAALRANETARVCRKQRSERRRWKPLPLSTVEMQIAASRKLQMTSEQAMNAAEALYTNGFISYPRTETDVFNPNIDLRAIVAEQAGDARWSGFVSQLLGGDGGNENNAQAGGIRFERPRAGKHDDQAHPPIHPLRCAPSSSGFGDPHQDRLFEYITRRFLASCSVDAVGSETLVEVQIGM-ERFSTKALIVERKGFYEV-FTYERWAERDLP-DLQEGDE-VSIDEMHLRTSETEPPPLLAESDLISLMDRHGIGTDATIAEHVKKIQERGYVTKNAQNRFVPMTLGLGLVSGFELCEVFLARPTVRAEQEAELKRVGTGEVHMDVMLSHAMDLYRTLFTSLLDRRSRFVSAFEPYF 638          

BLAST of Ggra9175.t1 vs. uniprot
Match: L1K3I3 (DNA topoisomerase n=1 Tax=Guillardia theta (strain CCMP2712) TaxID=905079 RepID=L1K3I3_GUITC)

HSP 1 Score: 499 bits (1286), Expect = 1.610e-165
Identity = 270/599 (45.08%), Postives = 383/599 (63.94%), Query Frame = 0

Query:    4 RILNVAEKPSAAKEIMNVLKGGQPAQSRHGRSRYNPIYEFNMSLQGRPSLMIFTSVTGHIKNTDFEDRYRKWGSCDPSQLLVPSQTRVEWYVSEERRPLAETLRTEARRADWLILWLDCDSEGERIAVDVADICKEAKRSLLVKRARFSAMTRGDLFRAVNQLDVPNERVARMVATRQEIDLRAGSAYTRFLTKQLEKYALTDMDKQVISYGPCQFPTLGLVVDRWLRIQNFVSRPFWVFELVL-KDCRVP-LEWFRKQLFDEYSAMTLYELCAEEAQADGNVATVKRVDKRVKTRWRPLPLSTVELQKVAARSLRINSHRTMEIAEALYNKGLISYPRTETNRFSRTYDLKGLIQKHVNHTTWGSFAERLLSPSTNQDPLTFLWPRSGPQDDGAHPPIHPTQGAPLSFDSTDHARVYQYITKRFLATCSIDAVGSETRTDVLVGSAEYFTAKGLIVEQKGYLEVLYPFEQWTDKDMPLSLLEIDACIPIESFTFRRSQTQPPPLLQEADLIALMDHHGIGTDATIAEHIRKVQDRRYVEKLAQGRFSPTEIGKALVIAHEQCQIHLARPHMRAKQENELKRILNGEIEAAQVLQSALE 600
            R+L VAEKPS AKE+  +L  G  AQ+ +  S+YN  +EF+  L GRP  ++ TSVTGH+   DF++ +RKW +C+P QLL PS  +V   + ++++ L   L  E RR   L+ +LDCD EGE I+ +V +IC++A   + V+RA FSA+   D+ RA+  L +PN+ ++  V  R EIDLR G+A+TRF T  L K     +  +++S+GPCQF TLG +VDR  +I+ FV   FW F   L +D +    +W R +LFD  +   +YE+  E+      +ATV  V +R+K RWRPLPLSTV LQ +A+R LR++S  TM+IAE LYNKG ISYPRTET +F    +L+ LI K      WG++A  LL+ S       F WPR+G  DD AHPPIHPTQ    +  S   A++Y+ + + FLA CS +A+G+ET  ++ +G  E F +KGL++++  +L+V YP+E+W+DKD+P+   E +   P E       +TQ PPLL EADLI+LMD HGIGTDATIAEHI KVQ R Y  K    +FSPT +GK+LV ++E   + LARPH RA+ E ++ +I +G+  A  V++  +E
Sbjct:    5 RVLCVAEKPSVAKELAAILSQGH-AQAFNTHSQYNRKFEFSYELDGRPCTLVVTSVTGHLMERDFDESHRKWHTCEPIQLLDPS-IQVIKMIPQDKKGLERNLLEEGRRCGQLVCFLDCDREGENISYEVIEICQKANPRIRVRRAHFSALIPRDIHRAMRTLTLPNQSLSEAVEARSEIDLRLGAAFTRFQTMVLGK-RFEQLSDKLLSWGPCQFATLGFIVDRAWKIERFVPEDFWSFRCNLSRDGKTAHFDWRRGRLFDRVACTLIYEMLMEDP-----IATVNSVHERLKERWRPLPLSTVNLQTIASRKLRMSSEETMQIAEELYNKGFISYPRTETEKFKEGTNLQELISKQCESGHWGNYANELLAGS-------FQWPRAGQNDDQAHPPIHPTQFT--NSLSGKQAQLYELVVRHFLACCSKNAIGAETTVEISIG-VEQFVSKGLMIKELNWLKV-YPYERWSDKDIPI-FTEGEQFEPTE-LAMTEGRTQAPPLLSEADLISLMDSHGIGTDATIAEHITKVQQRDYAIKSDSQQFSPTSLGKSLVTSYEAIGLELARPHFRARMEQDMTKIASGQTSARVVIEEWME 582          

BLAST of Ggra9175.t1 vs. uniprot
Match: A0A2P6RSQ3 (DNA topoisomerase n=5 Tax=Rosoideae TaxID=171638 RepID=A0A2P6RSQ3_ROSCH)

HSP 1 Score: 503 bits (1295), Expect = 1.460e-163
Identity = 285/657 (43.38%), Postives = 384/657 (58.45%), Query Frame = 0

Query:    4 RILNVAEKPSAAKEIMNVLKGGQPAQSRHGRSRYNPIYEFNMSLQGRPSLMIFTSVTGHIKNTDFEDRYRKWGSCDPSQLLVPSQTRVEWYVSEERRPLAETLRTEARRADWLILWLDCDSEGERIAVDVADICKEAKRSLLVKRARFSAMTRGDLFRAVNQLDVPNERVARMVATRQEIDLRAGSAYTRFLTKQLEKY----ALTDMDKQVISYGPCQFPTLGLVVDRWLRIQNFVSRPFWVFELVLKDCR--VPLEWFRKQLFDEYSAMTLYELCAEEAQADGNVATVKRVDKRVKTRWRPLPLSTVELQKVAARSLRINSHRTMEIAEALYNKGLISYPRTETNRFSRTYDLKGLIQKHVNHTTWGSFAERLLSPSTNQDPLTFLW--PRSGPQDDGAHPPIHPTQ-GAPLSFDSTDHARVYQYITKRFLATCSIDAVGSETRTDVLVGSAEYFTAKGLIVEQKGYLEVLYPFEQWTDKDMPLSLLEIDACIPIESFTFRRSQTQPPPLLQEADLIALMDHHGIGTDATIAEHIRKVQDRRYVEKLAQGRFSPTEIGKALVIAHEQCQIHLARPHMRAKQENELKRILNGEIEAAQVLQSALEEYRAKLSYLKTRRAVLHTVFSRHFSEVVSTLTPDLRGDELAGEGKGIREVG 651
            R+LNVAEKPS AK + N+L  GQ  + R GRSRYN IYEFN S+ G P  M+FTSVTGH+   +FEDRYRKW SCDP  L    Q  V  +V E++  +  TL  EAR   WL+LWLDCD EGE IA +V D+C    R L +KRARFSA+   D+ RAV  LD  N+  A  V  RQEIDLR G+++TRF T  L       + TD    V+SYGPCQFPTLG +V+R+  IQ+     FW      +         W R   FD   A+ +YE+C E+       ATV +V ++ K ++ P PL+T+EL+K A+R  R++S  TM++AE LY  G ISYPRTET+ FS   DL  ++Q+   H  WGS+A+RLL      DP + LW  P SG  DD AHPPIHPT+  +  S  S DH R+Y+ + + FLA  S  AVG+ET  ++ + + E F+A G ++  K YL+V Y FE W    +P   +     IP  + T     T+PPPLL EADL+  MD  GIGTDAT+ +HI+K+ DR Y  K +  RFSPT +G+ALV+ ++     L +P++RA  E ++K +  G    A+VL++ L++ +A     +  +  L       F     +  P   GDE    G+ IR  G
Sbjct:    6 RVLNVAEKPSVAKSVANILSRGQGLRMREGRSRYNKIYEFNYSINGCPCQMLFTSVTGHLMELEFEDRYRKWHSCDPVLLF---QAPVRKFVPEDKLDIKRTLEEEARMCQWLVLWLDCDREGENIAFEVIDVCTSVNRHLTIKRARFSALIDRDIHRAVQNLDNANKYFADAVDARQEIDLRIGASFTRFQTMLLRDAFVIDSATDDRNLVLSYGPCQFPTLGFIVERYWEIQSHEPEEFWTINCSHRSDEGIATFSWMRGHFFDHACAVIIYEMCVEDP-----TATVTKVRQQEKLKYPPYPLNTIELEKRASRYFRMSSEHTMKVAEELYQSGFISYPRTETDGFSPNTDLHAIVQEQQGHPGWGSYAQRLL------DPASGLWRNPSSGGHDDKAHPPIHPTKFSSGESGWSQDHNRLYELVVRHFLACVSQPAVGAETTVEIDI-AGEMFSASGRVILAKNYLDV-YRFESWGGSLLPTYAVG-QQFIPT-TLTLDSGVTRPPPLLSEADLLGCMDKEGIGTDATMHDHIKKLLDRFYATKDSNTRFSPTNLGEALVMGYDDMGYKLWKPYLRAVMERDMKAVSEGNKSKAEVLETCLQQMKACFVDARLNKVKLFEAMGVFFER---SNRPG--GDEQHASGEFIRRCG 639          

BLAST of Ggra9175.t1 vs. uniprot
Match: A0A2K2ACL1 (DNA topoisomerase n=10 Tax=Saliceae TaxID=238069 RepID=A0A2K2ACL1_POPTR)

HSP 1 Score: 498 bits (1283), Expect = 1.180e-161
Identity = 283/678 (41.74%), Postives = 401/678 (59.14%), Query Frame = 0

Query:    3 LRILNVAEKPSAAKEIMNVLKGGQPAQSRHGRSRYNPIYEFNMSLQGRPSLMIFTSVTGHIKNTDFEDRYRKWGSCDPSQLLVPSQTRVEWYVSEERRPLAETLRTEARRADWLILWLDCDSEGERIAVDVADICKEAKRSLLVKRARFSAMTRGDLFRAVNQLDVPNERVARMVATRQEIDLRAGSAYTRFLTKQLEKYALTDMDKQ----VISYGPCQFPTLGLVVDRWLRIQNFVSRPFWVFELVLKDCR--VPLEWFRKQLFDEYSAMTLYELCAEEAQADGNVATVKRVDKRVKTRWRPLPLSTVELQKVAARSLRINSHRTMEIAEALYNKGLISYPRTETNRFSRTYDLKGLIQKHVNHTTWGSFAERLLSPSTNQDPLTFLW--PRSGPQDDGAHPPIHPTQ-GAPLSFDSTDHARVYQYITKRFLATCSIDAVGSETRTDVLVGSAEYFTAKGLIVEQKGYLEVLYPFEQWTDKDMPLSLLEIDACIPIESFTFRRSQTQPPPLLQEADLIALMDHHGIGTDATIAEHIRKVQDRRYVEKLAQGRFSPTEIGKALVIAHEQCQIHLARPHMRAKQENELKRILNGEIEAAQVLQSALEEYRAKLSYLKTRRAVLHTVFSRHFSEVVSTLTPDLRGDELAGEGKGIREVGVVE---VDIKRVEVANGMIG 668
            +++LNVAEKPS AK +  +L   Q  + R GRSRYN I+EFN S+ G+   M+ TSVTGH+   +FEDR+RKW SCDP+ L       V  YV E++  +  TL  EARR  WL+LWLDCD EGE IA +V ++CK   R+L ++RARFSA+   D+  A   L  PN+  +  V  RQEIDLR G+++TRF T  L    + D  +     V+SYGPCQFPTLG VV+R+  IQ+     FW      +         W R  LFD  S++ LYE+C EE       ATV +V ++ K ++ P PLST+EL+K A+R  R++S +TM++AE LY  G ISYPRTET+ FS   DL  ++Q+H  H  WGS+A+RLL P         LW  P +G  DD AHPPIHPT+  A  S  S DH R+Y+ + + FLA  S  AVG+ET  ++ + + E F+A G ++  K YL+V Y FE W D  +P + ++    IP  + T     T+PPPLL EADL++ MD  GIGTDAT+ +HI+K+ DR YV K A  RFSPT +G+ALV+ ++     L +P++R+  E ++K +  G    A+VL + L++ +A     K  +  L    +  F+      +    GD+ +  G+ +R  G+ +   + +K+    N M+G
Sbjct:    2 IKVLNVAEKPSVAKSVATILSRNQ-LRVRDGRSRYNKIFEFNYSINGQQCHMLVTSVTGHLMEVEFEDRFRKWHSCDPADLYTAP---VRKYVPEDKLDIKRTLEEEARRCHWLVLWLDCDREGENIAFEVMEVCKGVNRNLTIRRARFSALIERDIHEAAQNLIAPNQWFSDAVDARQEIDLRIGASFTRFQTMLLRDRFVIDSAQDDRNLVLSYGPCQFPTLGFVVERYWEIQSHEPEEFWTINCTHRSDEGLASFNWMRGHLFDYTSSVILYEMCVEEP-----TATVTKVQQQEKLKYPPYPLSTIELEKRASRYFRMSSEQTMKVAEDLYQAGFISYPRTETDSFSSRTDLHTIVQEHQEHPVWGSYAQRLLDPGAG------LWRNPSNGGHDDKAHPPIHPTKFSAGESRWSQDHHRLYELVVRHFLACVSQPAVGAETVVEIDI-AGERFSASGRVILAKNYLDV-YRFESWGDSMIP-TYVQGQQFIPA-TLTLDSGVTRPPPLLSEADLLSCMDKAGIGTDATMHDHIKKLLDRFYVTKDANTRFSPTNLGEALVMGYDDMGYELWKPNLRSLMECDMKEVSVGNKSKAEVLATCLQQMKACFLDAKVNKVKLLEAMAIFFNR-----SDRSNGDDSSALGENVRPCGLCQEANMVLKKNRDGNFMVG 655          

BLAST of Ggra9175.t1 vs. uniprot
Match: A0A446SDJ8 (DNA topoisomerase n=2 Tax=Triticum turgidum subsp. durum TaxID=4567 RepID=A0A446SDJ8_TRITD)

HSP 1 Score: 487 bits (1253), Expect = 6.340e-161
Identity = 271/613 (44.21%), Postives = 377/613 (61.50%), Query Frame = 0

Query:    2 ALRILNVAEKPSAAKEIMNVL-KGGQPAQSRHGRSRYNPIYEFNMSLQGRPSLMIFTSVTGHIKNTDFEDRYRKWGSCDPSQLLVPSQTRVEWYVSEERRPLAETLRTEARRADWLILWLDCDSEGERIAVDVADICKEAKRSLLVKRARFSAMTRGDLFRAVNQLDVPNERVARMVATRQEIDLRAGSAYTRFLTKQLEKYALTDMDKQ----VISYGPCQFPTLGLVVDRWLRIQNFVSRPFWVFEL--VLKDCRVPLEWFRKQLFDEYSAMTLYELCAEEAQADGNVATVKRVDKRVKTRWRPLPLSTVELQKVAARSLRINSHRTMEIAEALYNKGLISYPRTETNRFSRTYDLKGLIQKHVNHTTWGSFAERLLSPSTNQDPLTFLW--PRSGPQDDGAHPPIHPTQGAPLSFD-STDHARVYQYITKRFLATCSIDAVGSETRTDVLVGSAEYFTAKGLIVEQKGYLEVLYPFEQWTDKDMPLSLLEIDACIPIESFTFRRSQTQPPPLLQEADLIALMDHHGIGTDATIAEHIRKVQDRRYVEKLAQGRFSPTEIGKALVIAHEQCQIHLARPHMRAKQENELKRILNGEIEAAQVLQSALEEYRA 604
            A+R+LNVAEKPS AK +  +L +GG   QSR GRSRYN ++EFN ++  +   M+ TSVTGH+   DF+DRYR+W SCDP++L       V   V ++++ +  TL  EAR   WL+LWLDCD EGE IA +V DIC  A R+L + RARFSA+   ++  AV  L  PN+  A  V  RQEIDLR G+++TRF T  L+   + D+  +    V+SYGPCQFPTLG +V+R+  IQ      FW         +      W R  LFD  SA+ +YE+C  E      +ATV+ V  + K ++ P PLSTVELQK A+R  R++S  TM++AE LY  G ISYPRTET+ FS   DL  ++++ V+H  WG++A+RLL+P         LW  P +G  DD AHPPIHPT+ +    + S DH +VY+ + + FLA CS  AVG+ET  +V + + E F A G +V  K YL+V Y ++ W    +P   +     +P  S T     T+PPPLL EADL++ MD  GIGTDAT+ EHI+K+ DR Y  K A  RFSPT +G+ALV+ +++    L +P++RA  E+++K +  G    AQVL+  L++ +A
Sbjct:    7 AIRVLNVAEKPSVAKSVAEILSRGGM--QSRAGRSRYNRVFEFNYAIGAQACHMLVTSVTGHLMELDFDDRYRRWYSCDPAELF---HAPVRKAVPQDKQDIKRTLEEEARTCQWLVLWLDCDREGENIAYEVVDICAGANRNLNIWRARFSALIPREIHEAVQHLARPNKLFADAVDARQEIDLRIGASFTRFQTMLLKDAFVLDVSGEERNMVLSYGPCQFPTLGFIVERFWEIQAHEPEEFWTINCSHTSDEGTASFIWIRGHLFDYSSAVVIYEMCVHEP-----MATVQNVRNQEKLKYPPYPLSTVELQKRASRCCRMSSEHTMKVAEELYQAGFISYPRTETDSFSPNTDLHAIVREQVDHPDWGTYAQRLLNPEER------LWRNPSNGGHDDKAHPPIHPTKFSTGENNWSPDHKKVYELVVRHFLACCSQPAVGAETTVEVDI-AGEQFNASGRVVLAKNYLDV-YRYDSWGGSLLPTYTIG-QQFVPT-SLTLDSGVTRPPPLLAEADLLSCMDKAGIGTDATMHEHIKKLLDRCYATKDANSRFSPTNLGEALVMGYDEMGYELWKPYLRAMMESDMKSVSVGTKSKAQVLEGCLQQMKA 599          

BLAST of Ggra9175.t1 vs. uniprot
Match: A0A5D6XRA3 (DNA topoisomerase n=1 Tax=Pythium brassicum TaxID=1485010 RepID=A0A5D6XRA3_9STRA)

HSP 1 Score: 497 bits (1280), Expect = 7.240e-161
Identity = 282/639 (44.13%), Postives = 397/639 (62.13%), Query Frame = 0

Query:    4 RILNVAEKPSAAKEIMNVLKGGQPAQSRHGRSRYNPIYEFNMSLQGRPSLMIFTSVTGHIKNTDFEDRYRKWGSCDPSQLL---VPSQTRVEWYVSEERRPLAETLRTEARRADWLILWLDCDSEGERIAVDVADICKEA-KRSLLVKRARFSAMTRGDLFRAVNQLDVPNERVARMVATRQEIDLRAGSAYTRFLTKQLE-KYALTDMDKQ-VISYGPCQFPTLGLVVDRWLRIQNFVSRPFWVFELVLKDCRVP------LEWFRKQLFDEYSAMTLYE-LCAEEAQADGNVATVKRVDKRVKTRWRPLPLSTVELQKVAARSLRINSHRTMEIAEALYNKGLISYPRTETNRFSRTYDLKGLIQKHVNHTTWGSF-AERLLSPSTNQDPLTFLWPRSGPQDDGAHPPIHPTQGAPL-SFDSTDHARVYQYITKRFLATCSIDAVGSETRTDVLVGSAEYFTAKGLIVEQKGYLEVLYPFEQWTDKDMPLSLLEIDACIPIESFTFRRSQTQPPPLLQEADLIALMDHHGIGTDATIAEHIRKVQDRRYVEKLAQG-RFSPTEIGKALVIAHEQCQIHLARPHMRAKQENELKRILNGEIEAAQVLQSALEEYRAKLSYLKTRRAVLHTVFSRHFSE 626
            ++LNVAEKPS AKEI  +L GGQ AQ R G S+YNP+YEF   LQG+   M  TSV+GH+   +F+  YR W SC P +L    V  +TR +    E +R + +TL  EAR++ WL+LWLDCD EGE IA +V  +C+ A  R L V RARFSA+   D+  A   L  PNE++A     R EIDLR G+A+TRF T +L+ ++     D+  VISYGPCQFPTLG VV+R+L +QNF   PF+  +L  +    P        W R ++FD  + + LYE L A+ A      A V+ V+KR   + +PLPL+TVELQK A+R LR++S +TM+ AE+LYNKG++SYPRTETN+F    D+  L++ H +   WG + ++ LL  ++  +P     PR G  DD AHPPIHPT+  PL S       +VY+++T+ FLA CS DAVG +T   V + + E F+  GL++ ++ YL++ Y +E W+   +P+   + D  +P  +      +T PPPLL EADLIALMD +GIGTDATIAEHI+ +  R Y EK+  G +F PT +G ALV+++E   + LA+P +RA  EN+ K I  G+ +  QV+Q+ +E+ +     +     VL   FS HF++
Sbjct:    5 KVLNVAEKPSVAKEISAILSGGQ-AQRRQGFSQYNPVYEFPYELQGQRVHMTITSVSGHLMELEFDANYRSWHSCSPVELFTAAVSKKTRAD----ETQRKIEKTLVNEARQSQWLVLWLDCDREGENIAFEVKSVCERAASRRLRVFRARFSALIPRDIVHAAQHLAAPNEQLALACDARSEIDLRIGAAFTRFQTMRLQSRFPALKKDRGGVISYGPCQFPTLGFVVERFLHVQNFEREPFFRLDLAHRQADQPGAPTTAFAWDRGRVFDRVACLCLYEALVAQPA------ARVEAVEKRPTWKRKPLPLTTVELQKRASRWLRVSSEQTMKAAESLYNKGVLSYPRTETNKFKEGTDVLALVRLHESSPQWGDYVSQTLLGGASKYEP-----PRHGGADDQAHPPIHPTKSVPLASIADAVERKVYEFVTRHFLACCSKDAVGHQTTVRVRI-AGEGFSTSGLMIAERNYLDI-YKYENWSASAIPV-YEQGDVFVP-SALAMAAGETAPPPLLSEADLIALMDSNGIGTDATIAEHIKTILKREYAEKVNDGTQFKPTTLGLALVLSYEHMGLDLAKPALRAAMENDCKAIALGQKDCRQVVQTCMEQMKEIFLSVTRNAQVLDQTFSEHFNQ 623