Gcaud2829.t1 (mRNA) Gracilaria caudata M_176_S67 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview


Name	Gcaud2829.t1
Unique Name	Gcaud2829.t1
Type	mRNA
Organism	Gracilaria caudata M_176_S67 male (Gracilaria caudata M_176_S67 male)
Sequence length	785

Alignments

The following features are aligned


Aligned Feature	Feature Type	Alignment Location
NODE_7_length_102599_cov_4.541909	contig	NODE_7_length_102599_cov_4.541909:17741..20095 +

Analyses

This mRNA is derived from or has results from the following analyses


Analysis Name	Date Performed
Gracilaria caudata M_176_S67 male OGS1.0	2022-05-09

Properties


Property Name	Value
Seed ortholog	4537.OPUNC02G29970.1
PFAMs	Cullin,Cullin_Nedd8
Max annot lvl	35493\|Streptophyta
KEGG ko	ko:K03869
KEGG Pathway	ko04120,ko04340,ko04341,map04120,map04340,map04341
KEGG Module	M00384
GOs	GO:0000151,GO:0003674,GO:0005488,GO:0005515,GO:0005575,GO:0005622,GO:0005623,GO:0005737,GO:0005829,GO:0008150,GO:0009314,GO:0009416,GO:0009628,GO:0009639,GO:0009909,GO:0009911,GO:0019899,GO:0031461,GO:0031625,GO:0032991,GO:0044389,GO:0044424,GO:0044444,GO:0044464,GO:0048518,GO:0048580,GO:0048582,GO:0048831,GO:0050789,GO:0050793,GO:0050896,GO:0051094,GO:0051239,GO:0051240,GO:0065007,GO:1902494,GO:1990234,GO:2000026,GO:2000241,GO:2000243
Evalue	1.03e-212
EggNOG OGs	COG5647@1\|root,KOG2166@2759\|Eukaryota,37I6E@33090\|Viridiplantae,3GEGV@35493\|Streptophyta,3KRMK@4447\|Liliopsida,3I2DA@38820\|Poales
Description	Belongs to the cullin family
COG category	D
BRITE	ko00000,ko00001,ko00002,ko03400,ko04121

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Species	Type	Position
Gcaud2829	Gcaud2829	Gracilaria caudata M_176_S67 male	gene	NODE_7_length_102599_cov_4.541909 17741..20095 +

The following start_codon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Species	Type	Position
Gcaud2829.t1.start1	Gcaud2829.t1.start1	Gracilaria caudata M_176_S67 male	start_codon	NODE_7_length_102599_cov_4.541909 17741..17743 +

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Species	Type	Position
Gcaud2829.t1.CDS1	Gcaud2829.t1.CDS1	Gracilaria caudata M_176_S67 male	CDS	NODE_7_length_102599_cov_4.541909 17741..20095 +

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Species	Type	Position
Gcaud2829.t1.exon1	Gcaud2829.t1.exon1	Gracilaria caudata M_176_S67 male	exon	NODE_7_length_102599_cov_4.541909 17741..20095 +

The following stop_codon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Species	Type	Position
Gcaud2829.t1.stop1	Gcaud2829.t1.stop1	Gracilaria caudata M_176_S67 male	stop_codon	NODE_7_length_102599_cov_4.541909 20093..20095 +

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Species	Type	Position
Gcaud2829.t1	Gcaud2829.t1	Gracilaria caudata M_176_S67 male	polypeptide	NODE_7_length_102599_cov_4.541909 17741..20095 +

Sequences

The following sequences are available for this feature:

mRNA sequence
mRNA - spliced transcript sequence
protein sequence
mRNA from alignment at NODE_7_length_102599_cov_4.541909:17741..20095+
coding sequence from alignment at NODE_7_length_102599_cov_4.541909:17741..20095+

mRNA sequence

>Gcaud2829.t1 ID=Gcaud2829.t1|Name=Gcaud2829.t1|organism=Gracilaria caudata M_176_S67 male|type=mRNA|length=785bp
MARRNSGRFVISCFPQPVQLDPHFAEKTWQTLEHAINEINKRNISNLSFE
QLYRYSYNMVLHKHGDYLYDGLTRLQTDHLIAVADLIKRTEPPVFLSEIK
RQWTWFEISLSHVREVLMYMDRHYVKPKKKKNVHELGLALFRDGVVRHPS
ILPRLSETLLASIDKERNGEPIDYLLVKSITRMLAELGVDDDGKSVYETV
FEDGFLERTRQFYAREATLYLSETTCSEYLRKASQRINQERLRVECYLEP
DTAPKVRRVAETELISKYMDKLINMENSGLIWMLRNDKIEDLKLMYTLFK
GIKHGEEEVRHNLKEEVLERGTKIIQNTENVRDPIALVNAILMLREKYDN
IVNTAFTTPAEKAPQASNPAGSAMTARYFLEQLNNGSSSDAVNGAQTRHE
SVPDKRFVSAVNDAFERFVNGFSRSAEYLSLYVDKLLRKDFKGLTDDEID
AKLDAVMALFRYLSDKDVFERYYKQHLQKRLLYSRSTSSDAERSFITKMK
TDCGYLYTSKMEIMFNDMKTSDESTSQFRDKVAQESIEMRGIDLKVFVLT
TMSWPVPQTPAVNLPEDAVHCASKFEEFYYQKHNGRRLTWQQSLGTAEIR
ARFGTRVYELYSVSAYAMCILLLFNSNDSLTYKEIADATLIPESELVRHL
QSLSLAKHKVLKKEPREKEVKPNDRFMFNSDFQSRSRRIKMQVVTAQKEN
ETERNHTRCRINADRGPVIDTVVVRIMKHRKTLEHNRLIAEVTRELESKF
EPNTQEIKKRIESLVEREYLERQKDKRQVYQYVA*

spliced messenger RNA

>Gcaud2829.t1 ID=Gcaud2829.t1|Name=Gcaud2829.t1|organism=Gracilaria caudata M_176_S67 male|type=mRNA|length=2355bp|location=Sequence derived from alignment at NODE_7_length_102599_cov_4.541909:17741..20095+ (Gracilaria caudata M_176_S67 male)|Notes=Excludes all bases but those of type(s): exon.  
ATGGCACGTCGTAACTCTGGACGTTTTGTCATCTCTTGTTTTCCGCAGCC
TGTTCAACTTGACCCCCACTTTGCGGAGAAGACATGGCAAACGCTTGAGC
ATGCCATCAATGAGATTAACAAACGCAACATTTCCAACCTTTCGTTTGAG
CAGCTTTATCGCTATTCGTACAACATGGTACTTCACAAGCACGGCGACTA
TCTGTACGATGGTCTTACTCGCCTGCAAACTGATCATCTCATCGCAGTTG
CAGATCTCATCAAGCGCACCGAACCCCCCGTCTTTCTGTCAGAGATAAAG
CGCCAGTGGACCTGGTTTGAAATCAGTCTCTCACATGTGCGCGAGGTTTT
GATGTACATGGATCGTCACTATGTCAAACCGAAGAAGAAGAAGAACGTAC
ATGAGCTAGGGCTTGCCCTCTTCCGCGACGGAGTCGTCCGTCACCCGTCC
ATTCTTCCACGTTTATCGGAGACTCTCTTGGCGAGCATCGATAAGGAGAG
AAATGGAGAACCTATTGATTACTTGCTTGTCAAGAGCATTACAAGAATGC
TGGCCGAACTGGGCGTAGACGACGACGGCAAATCTGTTTACGAGACCGTT
TTTGAAGATGGCTTTCTTGAGCGCACACGCCAGTTTTACGCCCGAGAAGC
CACTCTATATCTCTCGGAAACAACATGCTCTGAATATTTGCGAAAGGCTA
GTCAACGCATCAATCAAGAGCGTCTGAGAGTTGAATGCTATTTGGAGCCC
GACACCGCACCAAAAGTACGTCGCGTTGCAGAGACCGAACTCATATCCAA
GTACATGGACAAACTTATCAACATGGAGAACTCTGGTCTTATCTGGATGT
TGCGTAACGACAAAATTGAGGACCTTAAGCTCATGTACACTCTTTTCAAG
GGCATTAAACACGGTGAAGAGGAGGTCCGCCATAACCTCAAAGAAGAAGT
CCTAGAAAGAGGAACTAAAATTATCCAGAATACCGAAAACGTTCGCGATC
CTATTGCTCTGGTCAACGCTATCCTCATGTTAAGGGAAAAATACGACAAC
ATTGTCAACACTGCGTTTACGACTCCGGCCGAGAAGGCTCCGCAAGCCTC
AAACCCTGCAGGTTCAGCCATGACTGCTAGATATTTCTTAGAACAGCTGA
ACAATGGGTCCAGCAGCGATGCAGTGAATGGCGCCCAAACACGGCATGAA
AGCGTTCCTGATAAGCGCTTCGTTTCTGCGGTCAATGATGCATTTGAACG
GTTTGTAAACGGCTTCAGCCGATCTGCCGAATACCTTTCGCTCTATGTAG
ACAAACTCTTGCGGAAAGATTTCAAGGGACTAACTGATGATGAGATCGAT
GCCAAACTAGATGCTGTCATGGCACTGTTCCGGTATCTAAGTGACAAGGA
CGTTTTTGAACGATACTACAAGCAGCATTTACAAAAGCGACTGCTTTATT
CCCGATCTACCTCATCTGATGCGGAACGGAGTTTCATCACCAAGATGAAG
ACCGATTGCGGGTACTTGTACACTTCGAAAATGGAAATCATGTTCAACGA
TATGAAAACTAGTGACGAAAGCACATCACAGTTTAGGGACAAAGTGGCTC
AGGAATCTATTGAAATGCGAGGAATAGATTTGAAGGTGTTTGTGCTCACC
ACCATGAGTTGGCCGGTGCCTCAAACACCAGCTGTGAATCTCCCAGAAGA
TGCTGTACACTGCGCTTCTAAGTTTGAGGAATTCTATTACCAAAAGCATA
ACGGACGACGACTGACATGGCAGCAATCTCTCGGAACGGCAGAGATACGT
GCTAGGTTTGGCACCCGTGTATATGAGCTCTATTCGGTGTCGGCCTATGC
TATGTGCATCCTTTTGTTATTTAACTCGAATGACAGTCTTACATACAAAG
AAATCGCTGACGCTACCCTCATCCCTGAATCAGAGCTTGTGAGACACTTA
CAAAGTCTTTCTCTGGCGAAGCACAAGGTCTTGAAGAAAGAACCTCGGGA
AAAGGAAGTGAAACCAAACGATAGATTCATGTTCAACTCTGATTTTCAAA
GTCGAAGCAGAAGAATCAAGATGCAAGTCGTCACAGCTCAGAAAGAGAAC
GAAACTGAGAGAAATCATACGCGCTGTCGCATCAATGCCGACCGCGGTCC
TGTGATAGATACAGTTGTCGTGCGCATCATGAAGCATCGTAAGACTTTGG
AGCACAACAGACTTATAGCTGAAGTCACCCGAGAATTAGAATCAAAGTTC
GAGCCAAACACTCAAGAGATCAAGAAGAGAATTGAAAGTCTTGTAGAGAG
AGAGTACTTGGAGAGACAAAAGGACAAGAGGCAGGTGTATCAGTATGTGG
CGTAG

protein sequence of Gcaud2829.t1

>Gcaud2829.t1 ID=Gcaud2829.t1|Name=Gcaud2829.t1|organism=Gracilaria caudata M_176_S67 male|type=polypeptide|length=785bp
MARRNSGRFVISCFPQPVQLDPHFAEKTWQTLEHAINEINKRNISNLSFE
QLYRYSYNMVLHKHGDYLYDGLTRLQTDHLIAVADLIKRTEPPVFLSEIK
RQWTWFEISLSHVREVLMYMDRHYVKPKKKKNVHELGLALFRDGVVRHPS
ILPRLSETLLASIDKERNGEPIDYLLVKSITRMLAELGVDDDGKSVYETV
FEDGFLERTRQFYAREATLYLSETTCSEYLRKASQRINQERLRVECYLEP
DTAPKVRRVAETELISKYMDKLINMENSGLIWMLRNDKIEDLKLMYTLFK
GIKHGEEEVRHNLKEEVLERGTKIIQNTENVRDPIALVNAILMLREKYDN
IVNTAFTTPAEKAPQASNPAGSAMTARYFLEQLNNGSSSDAVNGAQTRHE
SVPDKRFVSAVNDAFERFVNGFSRSAEYLSLYVDKLLRKDFKGLTDDEID
AKLDAVMALFRYLSDKDVFERYYKQHLQKRLLYSRSTSSDAERSFITKMK
TDCGYLYTSKMEIMFNDMKTSDESTSQFRDKVAQESIEMRGIDLKVFVLT
TMSWPVPQTPAVNLPEDAVHCASKFEEFYYQKHNGRRLTWQQSLGTAEIR
ARFGTRVYELYSVSAYAMCILLLFNSNDSLTYKEIADATLIPESELVRHL
QSLSLAKHKVLKKEPREKEVKPNDRFMFNSDFQSRSRRIKMQVVTAQKEN
ETERNHTRCRINADRGPVIDTVVVRIMKHRKTLEHNRLIAEVTRELESKF
EPNTQEIKKRIESLVEREYLERQKDKRQVYQYVA*

mRNA from alignment at NODE_7_length_102599_cov_4.541909:17741..20095+

Legend: start_codonpolypeptideCDSexonstop_codon

Hold the cursor over a type above to highlight its positions in the sequence below.

>Gcaud2829.t1 ID=Gcaud2829.t1|Name=Gcaud2829.t1|organism=Gracilaria caudata M_176_S67 male|type=mRNA|length=2355bp|location=Sequence derived from alignment at NODE_7_length_102599_cov_4.541909:17741..20095+ (Gracilaria caudata M_176_S67 male)
ATGGCACGTCGTAACTCTGGACGTTTTGTCATCTCTTGTTTTCCGCAGCC
TGTTCAACTTGACCCCCACTTTGCGGAGAAGACATGGCAAACGCTTGAGC
ATGCCATCAATGAGATTAACAAACGCAACATTTCCAACCTTTCGTTTGAG
CAGCTTTATCGCTATTCGTACAACATGGTACTTCACAAGCACGGCGACTA
TCTGTACGATGGTCTTACTCGCCTGCAAACTGATCATCTCATCGCAGTTG
CAGATCTCATCAAGCGCACCGAACCCCCCGTCTTTCTGTCAGAGATAAAG
CGCCAGTGGACCTGGTTTGAAATCAGTCTCTCACATGTGCGCGAGGTTTT
GATGTACATGGATCGTCACTATGTCAAACCGAAGAAGAAGAAGAACGTAC
ATGAGCTAGGGCTTGCCCTCTTCCGCGACGGAGTCGTCCGTCACCCGTCC
ATTCTTCCACGTTTATCGGAGACTCTCTTGGCGAGCATCGATAAGGAGAG
AAATGGAGAACCTATTGATTACTTGCTTGTCAAGAGCATTACAAGAATGC
TGGCCGAACTGGGCGTAGACGACGACGGCAAATCTGTTTACGAGACCGTT
TTTGAAGATGGCTTTCTTGAGCGCACACGCCAGTTTTACGCCCGAGAAGC
CACTCTATATCTCTCGGAAACAACATGCTCTGAATATTTGCGAAAGGCTA
GTCAACGCATCAATCAAGAGCGTCTGAGAGTTGAATGCTATTTGGAGCCC
GACACCGCACCAAAAGTACGTCGCGTTGCAGAGACCGAACTCATATCCAA
GTACATGGACAAACTTATCAACATGGAGAACTCTGGTCTTATCTGGATGT
TGCGTAACGACAAAATTGAGGACCTTAAGCTCATGTACACTCTTTTCAAG
GGCATTAAACACGGTGAAGAGGAGGTCCGCCATAACCTCAAAGAAGAAGT
CCTAGAAAGAGGAACTAAAATTATCCAGAATACCGAAAACGTTCGCGATC
CTATTGCTCTGGTCAACGCTATCCTCATGTTAAGGGAAAAATACGACAAC
ATTGTCAACACTGCGTTTACGACTCCGGCCGAGAAGGCTCCGCAAGCCTC
AAACCCTGCAGGTTCAGCCATGACTGCTAGATATTTCTTAGAACAGCTGA
ACAATGGGTCCAGCAGCGATGCAGTGAATGGCGCCCAAACACGGCATGAA
AGCGTTCCTGATAAGCGCTTCGTTTCTGCGGTCAATGATGCATTTGAACG
GTTTGTAAACGGCTTCAGCCGATCTGCCGAATACCTTTCGCTCTATGTAG
ACAAACTCTTGCGGAAAGATTTCAAGGGACTAACTGATGATGAGATCGAT
GCCAAACTAGATGCTGTCATGGCACTGTTCCGGTATCTAAGTGACAAGGA
CGTTTTTGAACGATACTACAAGCAGCATTTACAAAAGCGACTGCTTTATT
CCCGATCTACCTCATCTGATGCGGAACGGAGTTTCATCACCAAGATGAAG
ACCGATTGCGGGTACTTGTACACTTCGAAAATGGAAATCATGTTCAACGA
TATGAAAACTAGTGACGAAAGCACATCACAGTTTAGGGACAAAGTGGCTC
AGGAATCTATTGAAATGCGAGGAATAGATTTGAAGGTGTTTGTGCTCACC
ACCATGAGTTGGCCGGTGCCTCAAACACCAGCTGTGAATCTCCCAGAAGA
TGCTGTACACTGCGCTTCTAAGTTTGAGGAATTCTATTACCAAAAGCATA
ACGGACGACGACTGACATGGCAGCAATCTCTCGGAACGGCAGAGATACGT
GCTAGGTTTGGCACCCGTGTATATGAGCTCTATTCGGTGTCGGCCTATGC
TATGTGCATCCTTTTGTTATTTAACTCGAATGACAGTCTTACATACAAAG
AAATCGCTGACGCTACCCTCATCCCTGAATCAGAGCTTGTGAGACACTTA
CAAAGTCTTTCTCTGGCGAAGCACAAGGTCTTGAAGAAAGAACCTCGGGA
AAAGGAAGTGAAACCAAACGATAGATTCATGTTCAACTCTGATTTTCAAA
GTCGAAGCAGAAGAATCAAGATGCAAGTCGTCACAGCTCAGAAAGAGAAC
GAAACTGAGAGAAATCATACGCGCTGTCGCATCAATGCCGACCGCGGTCC
TGTGATAGATACAGTTGTCGTGCGCATCATGAAGCATCGTAAGACTTTGG
AGCACAACAGACTTATAGCTGAAGTCACCCGAGAATTAGAATCAAAGTTC
GAGCCAAACACTCAAGAGATCAAGAAGAGAATTGAAAGTCTTGTAGAGAG
AGAGTACTTGGAGAGACAAAAGGACAAGAGGCAGGTGTATCAGTATGTGG
CGTAG

Coding sequence (CDS) from alignment at NODE_7_length_102599_cov_4.541909:17741..20095+

>Gcaud2829.t1 ID=Gcaud2829.t1|Name=Gcaud2829.t1|organism=Gracilaria caudata M_176_S67 male|type=CDS|length=2355bp|location=Sequence derived from alignment at NODE_7_length_102599_cov_4.541909:17741..20095+ (Gracilaria caudata M_176_S67 male)
ATGGCACGTCGTAACTCTGGACGTTTTGTCATCTCTTGTTTTCCGCAGCC
TGTTCAACTTGACCCCCACTTTGCGGAGAAGACATGGCAAACGCTTGAGC
ATGCCATCAATGAGATTAACAAACGCAACATTTCCAACCTTTCGTTTGAG
CAGCTTTATCGCTATTCGTACAACATGGTACTTCACAAGCACGGCGACTA
TCTGTACGATGGTCTTACTCGCCTGCAAACTGATCATCTCATCGCAGTTG
CAGATCTCATCAAGCGCACCGAACCCCCCGTCTTTCTGTCAGAGATAAAG
CGCCAGTGGACCTGGTTTGAAATCAGTCTCTCACATGTGCGCGAGGTTTT
GATGTACATGGATCGTCACTATGTCAAACCGAAGAAGAAGAAGAACGTAC
ATGAGCTAGGGCTTGCCCTCTTCCGCGACGGAGTCGTCCGTCACCCGTCC
ATTCTTCCACGTTTATCGGAGACTCTCTTGGCGAGCATCGATAAGGAGAG
AAATGGAGAACCTATTGATTACTTGCTTGTCAAGAGCATTACAAGAATGC
TGGCCGAACTGGGCGTAGACGACGACGGCAAATCTGTTTACGAGACCGTT
TTTGAAGATGGCTTTCTTGAGCGCACACGCCAGTTTTACGCCCGAGAAGC
CACTCTATATCTCTCGGAAACAACATGCTCTGAATATTTGCGAAAGGCTA
GTCAACGCATCAATCAAGAGCGTCTGAGAGTTGAATGCTATTTGGAGCCC
GACACCGCACCAAAAGTACGTCGCGTTGCAGAGACCGAACTCATATCCAA
GTACATGGACAAACTTATCAACATGGAGAACTCTGGTCTTATCTGGATGT
TGCGTAACGACAAAATTGAGGACCTTAAGCTCATGTACACTCTTTTCAAG
GGCATTAAACACGGTGAAGAGGAGGTCCGCCATAACCTCAAAGAAGAAGT
CCTAGAAAGAGGAACTAAAATTATCCAGAATACCGAAAACGTTCGCGATC
CTATTGCTCTGGTCAACGCTATCCTCATGTTAAGGGAAAAATACGACAAC
ATTGTCAACACTGCGTTTACGACTCCGGCCGAGAAGGCTCCGCAAGCCTC
AAACCCTGCAGGTTCAGCCATGACTGCTAGATATTTCTTAGAACAGCTGA
ACAATGGGTCCAGCAGCGATGCAGTGAATGGCGCCCAAACACGGCATGAA
AGCGTTCCTGATAAGCGCTTCGTTTCTGCGGTCAATGATGCATTTGAACG
GTTTGTAAACGGCTTCAGCCGATCTGCCGAATACCTTTCGCTCTATGTAG
ACAAACTCTTGCGGAAAGATTTCAAGGGACTAACTGATGATGAGATCGAT
GCCAAACTAGATGCTGTCATGGCACTGTTCCGGTATCTAAGTGACAAGGA
CGTTTTTGAACGATACTACAAGCAGCATTTACAAAAGCGACTGCTTTATT
CCCGATCTACCTCATCTGATGCGGAACGGAGTTTCATCACCAAGATGAAG
ACCGATTGCGGGTACTTGTACACTTCGAAAATGGAAATCATGTTCAACGA
TATGAAAACTAGTGACGAAAGCACATCACAGTTTAGGGACAAAGTGGCTC
AGGAATCTATTGAAATGCGAGGAATAGATTTGAAGGTGTTTGTGCTCACC
ACCATGAGTTGGCCGGTGCCTCAAACACCAGCTGTGAATCTCCCAGAAGA
TGCTGTACACTGCGCTTCTAAGTTTGAGGAATTCTATTACCAAAAGCATA
ACGGACGACGACTGACATGGCAGCAATCTCTCGGAACGGCAGAGATACGT
GCTAGGTTTGGCACCCGTGTATATGAGCTCTATTCGGTGTCGGCCTATGC
TATGTGCATCCTTTTGTTATTTAACTCGAATGACAGTCTTACATACAAAG
AAATCGCTGACGCTACCCTCATCCCTGAATCAGAGCTTGTGAGACACTTA
CAAAGTCTTTCTCTGGCGAAGCACAAGGTCTTGAAGAAAGAACCTCGGGA
AAAGGAAGTGAAACCAAACGATAGATTCATGTTCAACTCTGATTTTCAAA
GTCGAAGCAGAAGAATCAAGATGCAAGTCGTCACAGCTCAGAAAGAGAAC
GAAACTGAGAGAAATCATACGCGCTGTCGCATCAATGCCGACCGCGGTCC
TGTGATAGATACAGTTGTCGTGCGCATCATGAAGCATCGTAAGACTTTGG
AGCACAACAGACTTATAGCTGAAGTCACCCGAGAATTAGAATCAAAGTTC
GAGCCAAACACTCAAGAGATCAAGAAGAGAATTGAAAGTCTTGTAGAGAG
AGAGTACTTGGAGAGACAAAAGGACAAGAGGCAGGTGTATCAGTATGTGG
CGTAG