Gcaud8767.t1 (polypeptide) Gracilaria caudata M_176_S67 male

BLAST of Gcaud8767.t1 vs. uniprot
Match: A0A2V3IUQ5_9FLOR (tRNA-5-taurinomethyluridine 2-sulfurtransferase n=1 Tax=Gracilariopsis chorda TaxID=448386 RepID=A0A2V3IUQ5_9FLOR)

HSP 1 Score: 766 bits (1979), Expect = 1.550e-275
Identity = 363/508 (71.46%), Postives = 424/508 (83.46%), Query Frame = 0

Query:    6 EDKLTASTRVSGCTSITHISIHLSDEGTISIRGYSDARISRGLVALFALGLHGCSTKDVLSLNLREVLHKSDLPGVIGSSRINSLGNILRAARKQVQMLTENASILRNDTAHILTGRWSERQGEDVAILLSGGVDSSVAMRLALESGARPHPFYLKIWLEDELAHMGECPWEEDIEYANAVCNQVGIKLQDIPFQRAYWDEVVSYTVQEAQKGRTPNPDVMCNSRIKFGAFYDRIGKYYKKVVTGHYAQTARDEMGRAELRLSPDELKDQTYFLAHLRQDQLATARFPVGKHTKEEVRMLAEHYDLPNKNRRDSQGICFLGKLKFDDFLGHHLGQKKGSLVEFETGQELGHHNGFWFFTLGQRRGVGLSGGPWYVVAKNVSQNIVYVSKDYNNVSKERNCFEFDSALWISGEWPAKLQHVGDKIKLRVKTRHGPRFHDATLTRLDDERGRVDLEGRDKGLAPGQFSAFYDIEGRCLGSGVIMSDVSMSDAPVAIKSLHYLPKKLQAHV 513
            E+ L+ S+RV GCTS+TH+ + L+ +  I I GYSDARISRGL+ALF +GLH CST+D L ++  E+L  + LP  IGSSRI  L +ILR  +K+V+ L  + + L       L GRWS+RQGEDVA+LLSGGVDSSVAMRL +ESGARPHPFY+KIWL+DE+AHMGECPWEEDIEYA+AVC Q G+KLQD+PFQRAYWDEVVSYTV+EA++GRTPNPD+MCNSRIKFGAFY+RIGK++ ++VTGHYAQ  RD  GR ELR SPD +KDQTYFLAHL+Q+QL T  FPVG++TKE+VR  A+HYDLPN+NRRDSQGICFLGKLKFD+FLGHHLG +KGSLVEFETG+ELG+HNGFWFFTLGQRRGVGLSGGPWYVVAK+VSQN+VYVSKDYN VSKERN FEFD ALWISG WP  LQ  GD +KLRVKTRHGP FH+A +TRLDDERG V+LE RDKGLA GQFSAFYD EGRCLGSG I  D+S+ D P  I+ L     +LQAHV
Sbjct:    6 EESLSPSSRVPGCTSVTHLIVKLNQDNCIEIEGYSDARISRGLLALFVIGLHRCSTEDALKVDASELLAATALPETIGSSRITGLASILRELQKRVRALESSKTTLLGCNDQDLAGRWSDRQGEDVAVLLSGGVDSSVAMRLIMESGARPHPFYIKIWLDDEMAHMGECPWEEDIEYASAVCRQAGVKLQDVPFQRAYWDEVVSYTVEEARRGRTPNPDIMCNSRIKFGAFYNRIGKHFDRIVTGHYAQAIRDLNGRVELRTSPDVMKDQTYFLAHLKQEQLKTVCFPVGRYTKEKVRQFAQHYDLPNQNRRDSQGICFLGKLKFDEFLGHHLGYRKGSLVEFETGKELGYHNGFWFFTLGQRRGVGLSGGPWYVVAKDVSQNVVYVSKDYNGVSKERNRFEFDKALWISGRWPPGLQASGDNLKLRVKTRHGPSFHNAIVTRLDDERGLVELEERDKGLAAGQFSAFYDYEGRCLGSGTISCDISILDLPREIQRLTSDSVRLQAHV 513          

BLAST of Gcaud8767.t1 vs. uniprot
Match: R7QEW1_CHOCR (tRNA-5-taurinomethyluridine 2-sulfurtransferase n=1 Tax=Chondrus crispus TaxID=2769 RepID=R7QEW1_CHOCR)

HSP 1 Score: 656 bits (1693), Expect = 2.340e-230
Identity = 318/511 (62.23%), Postives = 389/511 (76.13%), Query Frame = 0

Query:    6 EDKLTASTRVSGCTSITHISIHLSDEGTISIRGYSDARISRGLVALFALGLHGCSTKDVLSLNLREVLHKSDLPGVIGSSRINSLGNILRAARKQVQMLT--ENASILRNDTAHILTGRWSERQGEDVAILLSGGVDSSVAMRLALESGARPHPFYLKIWLEDELAHMGECPWEEDIEYANAVCNQVGIKLQDIPFQRAYWDEVVSYTVQEAQKGRTPNPDVMCNSRIKFGAFYDRIGKYYKKVVTGHYA-QTARDEMGRAELRLSPDELKDQTYFLAHLRQDQLATARFPVGKHTKEEVRMLAEHYDLPNKNRRDSQGICFLGKLKFDDFLGHHLGQKKGSLVEFETGQELGHHNGFWFFTLGQRRGVGLSGGPWYVVAKNVSQNIVYVSKDYNNVSKERNCFEFDSALWISGEWPAKLQHVGDKIKLRVKTRHGPRFHDATLTRLDDERGRVDLEGRDKGLAPGQFSAFYDIEGRCLGSGVIMSDVSMSDAPVAIKSLHYLPKKLQAHV 513
            +++L  STRVSGCTSI+H+ + +SD+ T+ +RG+SD+ ++RGL+A   LGL GC+  +V ++   E+L  S L G  G SR+  L +IL   + +V+ML   E          H + GRWS RQG+DVA+LLSGGVDSSVA+    ESGARPH FYLKIWLEDELAH+G CPWEED+EYA AVC Q  +KL D+PFQ+AYWDEVVSYTV EA++GRTPNPDVMCNSRIKFGAF++RIG+YY +V TGHYA QT   E G AELRLS D  KDQTYFLAHLRQ+Q+A + FP+G   K EVR LA+ YDLPNK R+DSQGICFLGKLKFDDFLGHHLG++ GSLVEFETG+ELG H GFWFFT+GQRRG+GLSGGPWYVV K+V +N+VYVSK+Y++  ++R  FEF++ LWI+GEWP  L   G +  LRVKTRHGP+FH+A +TR   E G V+L   DKGLAPGQFSAFYD E RCLGS VI +DV ++DAP  I        KLQ H+
Sbjct:  114 DNELHPSTRVSGCTSISHVEVVMSDDRTVKMRGFSDSMVARGLMAFVILGLDGCTADEVAAVMPLELLEASGLRGSAGPSRMMGLTSILSYVQNKVEMLRSGEKRDHAEKSEKHPMDGRWSGRQGKDVAVLLSGGVDSSVALHQIQESGARPHAFYLKIWLEDELAHLGSCPWEEDMEYAAAVCQQANVKLTDVPFQKAYWDEVVSYTVSEARRGRTPNPDVMCNSRIKFGAFFNRIGRYYDRVATGHYARQTVNPETGLAELRLSADMWKDQTYFLAHLRQEQIAASSFPLGNIPKNEVRELAKAYDLPNKTRKDSQGICFLGKLKFDDFLGHHLGREMGSLVEFETGRELGQHKGFWFFTIGQRRGIGLSGGPWYVVCKDVERNVVYVSKEYHSEKQQRTWFEFETPLWIAGEWPNALGFNGAQTALRVKTRHGPKFHNAVVTRTWAEGGEVELAEGDKGLAPGQFSAFYDDEDRCLGSAVIANDVHLADAPTDIIRRRTSAGKLQGHI 624          

BLAST of Gcaud8767.t1 vs. uniprot
Match: A0A7S2ZLQ9_9RHOD (tRNA-5-taurinomethyluridine 2-sulfurtransferase n=4 Tax=Rhodosorus marinus TaxID=101924 RepID=A0A7S2ZLQ9_9RHOD)

HSP 1 Score: 514 bits (1324), Expect = 2.630e-175
Identity = 264/509 (51.87%), Postives = 343/509 (67.39%), Query Frame = 0

Query:    6 EDKLTASTRVSGCTSITHISIHLSDEGTISIRGYSDARISRGLVALFALGLHGCSTKDVLSLNLREVLHKSDLPGVIGSSRINSLGNILRAARKQVQMLTENASILRNDTAHILTGRWSERQGEDVAILLSGGVDSSVAMRLALESGARPHPFYLKIWLEDELAHMGECPWEEDIEYANAVCNQVGIKLQDIPFQRAYWDEVVSYTVQEAQKGRTPNPDVMCNSRIKFGAFYDRIGKYYKKVVTGHYAQTARDEMGR-AELRLSPDELKDQTYFLAHLRQDQLATARFPVGKHTKEEVRMLAEHYDLPNKNRRDSQGICFLGKLKFDDFLGHHLGQKKGSLVEFETGQELGHHNGFWFFTLGQRRGVGLSGGPWYVVAKNVSQNIVYVSKDYNNVSKERNCFEFDSALWISGEWPAKLQHVGDKIKLRVKTRHGPRFHDATLTRLDDERGRVDLEGRDK-GLAPGQFSAFYDIEGRCLGSGVIMSDVSMSDAPVAIKSLHYLPKKLQAH 512
            ED      RV GCTSI H+      +G  +I+G SDA+++ GL+ + + GL+G   + +L +   E+   + L   + SSR N+  N+L+    Q+Q  T   S     ++   T   SE QGEDVA+LLSGGVDSSVA+RL LE G RP PFYLKIWLEDEL+H+GECPWEED+ YA +VC Q+ +K++ +  Q+ Y D VV YT+Q+A+ GRTPNPDVMCNSR+KFG F DR G+ + KV +GHYA+T R++     EL  S DE KDQTYFLAHL+Q Q+    FP+G +TK  VR LA+ Y+LPNK R+DSQGICFLGKLKFD+FL HHLG++ G  +E+ETG  LG H GFWF+TLGQR+G+GLSGGPWYVV+KN  +NIVY+S  Y+++ K R  F  +S  WISG  P + +       LRVK RHGPR     L  L+D  G V+LE ++  GLAPGQF+AFY +   CLGSG+I +D  +  AP A  S     K+LQAH
Sbjct:   67 EDICRPEYRVPGCTSIVHVKAVHDLDGKATIQGCSDAKLTAGLIRMLSEGLNGLDKEVILGMEFSELADATQLNSFMSSSRANAFSNVLKCIHTQLQGGTTGES----QSSSRPTTFVSEGQGEDVAVLLSGGVDSSVALRLLLEQGYRPTPFYLKIWLEDELSHLGECPWEEDLSYAESVCEQLNLKVEAVSLQQEYMDSVVKYTIQDARAGRTPNPDVMCNSRVKFGVFLDRFGEDFSKVASGHYARTRRNQRSEMTELVCSADEWKDQTYFLAHLKQSQVENTLFPIGAYTKARVRELADEYNLPNKARKDSQGICFLGKLKFDEFLEHHLGRQPGLFLEYETGLVLGKHRGFWFYTLGQRKGLGLSGGPWYVVSKNAEENIVYISGKYHDMDKPRTLFTVESLSWISGTPPTQEECAA----LRVKIRHGPRTVPCRLV-LEDNTGHVELEEKESIGLAPGQFAAFY-LGDVCLGSGIISADSGLEHAPKA--SSEVSSKRLQAH 563          

BLAST of Gcaud8767.t1 vs. uniprot
Match: A0A5J4Z0A3_PORPP (tRNA-5-taurinomethyluridine 2-sulfurtransferase n=1 Tax=Porphyridium purpureum TaxID=35688 RepID=A0A5J4Z0A3_PORPP)

HSP 1 Score: 495 bits (1275), Expect = 7.450e-167
Identity = 266/542 (49.08%), Postives = 353/542 (65.13%), Query Frame = 0

Query:    4 IPEDKLTASTRVSGCTSITHISIHL---------SDEGTISIRGYSDARISRGLVALFALGLHGCSTKDVLSLNLREVLHKSDLPGVIGSSRINSLGNILRAARKQVQMLTENAS-ILRNDTAH-----------------ILTGRWSERQGEDVAILLSGGVDSSVAMRLALESGARPHPFYLKIWLEDELAHMGECPWEEDIEYANAVCNQVGIKLQDIPFQRAYWDEVVSYTVQEAQKGRTPNPDVMCNSRIKFGAFYDRIGKYYKKVVTGHYAQTARDEM--GRAELRLSPDELKDQTYFLAHLRQDQLATARFPVGKHTKEEVRMLAEHYDLPNKNRRDSQGICFLGKLKFDDFLGHHLGQKKGSLVEFETGQELGHHNGFWFFTLGQRRGVGLSGGPWYVVAKNVSQNIVYVSKDYNNVSKERNCFEFDSALWIS-GEWPAKLQHVGDKIKLRVKTRHGPRFHDATLT--RLDDERGRVDLEGRDKGLAPGQFSAFYDIEGR-CLGSGVIMSDVSMSDAPVAIKSLHYLPKKLQAH 512
            +P    +A+TRV GCTS+T++   +              I + G SDA+++ GL+ + A G+  CS  ++L++++ E    + L   +  SR N L NI++  ++Q++  T + S ++RN  A                  IL GR+S    E+VA+LLSGGVDSSVA+RL LE G +  PFYLKIWL+DEL+H+ ECPWEED+ YA AVC Q G++L  +  Q  YWD+VVSYT+ EA+ GRTPNPDVMCNSRIKFG F DR+GK++K+V +GHYA+   DE   G+  L  S D  KDQTYFLA+L   QL+ A FP+G + K  VRMLAE +DLPNK+R+DSQGICFLGKL FD FL H+LG + G+LV+++TG +LG H GFWF+TLGQR+G+GL+GGPW+VV+K+  QNIVYVS  Y+   KER+ F      WI      A L     +  L VK RHGP+ H  TL+   LD  RG V LE R+ G+APGQF+ FY  +G  CLGSGVI +D  ++ AP  I    + PKKLQAH
Sbjct:  100 MPALHKSAATRVPGCTSVTYVQARVVAPSDPQKGESRQEIVLYGSSDAKLTTGLLRILADGMAQCSVDEILAMDVGEFARSAGLLDALTPSRNNGLRNIVQLIQQQLREETPDPSQVVRNGQASNMKSAVPLSSVDSTMDDILHGRYSGETREEVAVLLSGGVDSSVALRLVLEQGYKVVPFYLKIWLQDELSHLNECPWEEDVAYATAVCEQAGLQLHTLDLQHDYWDQVVSYTISEARAGRTPNPDVMCNSRIKFGVFLDRVGKHFKRVASGHYARIEIDETVPGKLLLSRSVDTHKDQTYFLANLTTAQLSKAMFPIGMYDKNTVRMLAERFDLPNKSRKDSQGICFLGKLPFDAFLEHYLGTQAGALVDYDTGIQLGSHRGFWFYTLGQRKGIGLAGGPWFVVSKDPQQNIVYVSNKYDAEDKERDSFGVQHLNWIGRASEEASLLETPTQ-DLIVKIRHGPKLHKCTLSLDALDPSRGSVKLEERNSGIAPGQFAVFY--KGPVCLGSGVISTDHGLASAPPQITG--FAPKKLQAH 636          

BLAST of Gcaud8767.t1 vs. uniprot
Match: A0A7S0EE01_9CRYP (tRNA-5-taurinomethyluridine 2-sulfurtransferase n=1 Tax=Hanusia phi TaxID=3032 RepID=A0A7S0EE01_9CRYP)

HSP 1 Score: 431 bits (1107), Expect = 1.950e-141
Identity = 226/515 (43.88%), Postives = 317/515 (61.55%), Query Frame = 0

Query:    4 IPEDKLTASTRVSGCTSIT--HISIHLSDEGT--ISIRGYSDARISRGLVALFALGLHGCSTKDVLSLNLREVLHKSDLPGVIGSSRINSLGNILRAARKQVQMLTENASILRNDTAHIL---------TGRWSERQGEDVAILLSGGVDSSVAMRLALESGARPHPFYLKIWLEDELAHMGECPWEEDIEYANAVCNQVGIKLQDIPFQRAYWDEVVSYTVQEAQKGRTPNPDVMCNSRIKFGAFYDRIGKYYKKVVTGHYAQTARD--EMGRAELRLSPDELKDQTYFLAHLRQDQLATARFPVGKHTKEEVRMLAEHYDLPNKNRRDSQGICFLGKLKFDDFLGHHLGQKKGSLVEFETGQELGHHNGFWFFTLGQRRGVGLSG------GPWYVVAKNVSQNIVYVSKDYNNVSKERNCFEFDSALWISGEWPAKLQHVGDKIKLRVKTRHGPRFHDATLTRLDDER--GRVDLEGRDKGLAPGQFSAFYDIEGRCLGSGVIMSDVSMSDA 495
            +PE K     RV GC S    ++S  + + G+  +S+ G +DAR++RGL+AL                     +  ++L   + + R + L ++L   +  ++                          T  W  R  E+VA+LLSGGVDSSVA+ L    G +P  FYL+IWLEDELAH+GECPWEED  YA +V  ++ + ++ I  Q  YW++VV Y + EA+ GRTPNPD+MCNSRIKFG F   +G+++ +V +GHYA +  D  E G + L +SPD++KDQTYFL +LRQ+QL  A FP+G +TKE+VR LA  +DLP + R+DSQGICFLGKLKF+DF+GHHLG+  G +V+F TG+ELG H G WF+T+GQR+G+G +       GPW+V  K+  +N++YVS  Y+ +   R  F+ +S  WISGE P       ++++LR+KTRHGP  H+  LT L   R  GRV LEGRD GLAPGQ++A YD E  C+G G+I     ++ A
Sbjct:  127 MPEIK--ERVRVPGCISNVQVYVSSQVDELGSLRVSLGGDADARVARGLLALLVXXXXXXXXXXXXXXXXXXXIQSANLKAFLPTGRNDGLRSMLTTFKSLIEQERXXXXXXXXXXXXXXXXXXXXXQQTWAWGGRS-EEVAVLLSGGVDSSVALELIRRQGLKPRAFYLRIWLEDELAHLGECPWEEDWSYATSVAERLNVPIESISLQEEYWEQVVDYLIGEAKLGRTPNPDIMCNSRIKFGMFEKYVGQHFSRVASGHYAVSLHDDKEGGVSRLLMSPDKIKDQTYFLCNLRQEQLKHALFPIGGYTKEQVRELAREFDLPTQKRKDSQGICFLGKLKFEDFVGHHLGENPGPVVDFHTGRELGEHRGLWFYTIGQRKGLGSATRRVTHLGPWFVAGKDRDKNVLYVSNQYDTIDGPRRGFDIESINWISGEAPRD-----EEVRLRIKTRHGPGIHNGLLT-LSPGRLSGRVSLEGRDSGLAPGQWAALYDGE-ECVGGGMIADSTFLTKA 631          

BLAST of Gcaud8767.t1 vs. uniprot
Match: L1IST2_GUITC (tRNA-5-taurinomethyluridine 2-sulfurtransferase n=3 Tax=Guillardia theta TaxID=55529 RepID=L1IST2_GUITC)

HSP 1 Score: 418 bits (1074), Expect = 1.770e-139
Identity = 196/373 (52.55%), Postives = 266/373 (71.31%), Query Frame = 0

Query:  123 WSERQGEDVAILLSGGVDSSVAMRLALESGARPHPFYLKIWLEDELAHMGECPWEEDIEYANAVCNQVGIKLQDIPFQRAYWDEVVSYTVQEAQKGRTPNPDVMCNSRIKFGAFYDRIGKYYKKVVTGHYAQTARDEMGR--AELRLSPDELKDQTYFLAHLRQDQLATARFPVGKHTKEEVRMLAEHYDLPNKNRRDSQGICFLGKLKFDDFLGHHLGQKKGSLVEFETGQELGHHNGFWFFTLGQRRGVGLSG------GPWYVVAKNVSQNIVYVSKDYNNVSKERNCFEFDSALWISGEWPAKLQHVGDKIKLRVKTRHGPRFHDATLTRLDDE-RGRVDLEGRDKGLAPGQFSAFYDIEGRCLGSGVI 486
            W  R  E+VA+LLSGGVDSSVA+ L    G +P  FYL+IWLEDELAH+GECPWEED  YA++V  Q+ + L+ I  Q  YW++VV Y ++EA+ GRTPNPD+MCNSRIKFG F + +GK++ +V +GHYA +  D++G   + L +SPD++KDQTYFL +LRQDQL  A FP+G +TKE+VR LA  ++LP + R+DSQGICFLGKLKF+DF+GHHLG+  G +++F TG+ELG H G WF+T+GQR+G+G +       GPW+V  K+  +N++YVS  Y+ +   R  F+ +   W++G  P       D ++LR+KTRHGP  HD  LT    +  GRV L+GRD GLAPGQ++A YD E  C+G G+I
Sbjct:   39 WGGRS-EEVAVLLSGGVDSSVALELVRRQGFKPRAFYLRIWLEDELAHLGECPWEEDWSYASSVAEQLKVPLESISLQEEYWEQVVEYLIREAKMGRTPNPDIMCNSRIKFGMFEEYVGKHFSRVASGHYAVSCHDDVGTRPSRLMMSPDKVKDQTYFLCNLRQDQLKHALFPIGGYTKEKVRELAREFNLPTQRRKDSQGICFLGKLKFEDFVGHHLGEDPGPVIDFHTGRELGEHRGLWFYTIGQRKGLGSATRKVTHLGPWFVAGKDRERNMLYVSNQYDVIEGPRRSFDVEQINWMAGSLPED-----DALRLRIKTRHGPNIHDGLLTLSQGKLSGRVSLDGRDSGLAPGQWAALYDGE-ECIGGGMI 404          

BLAST of Gcaud8767.t1 vs. uniprot
Match: A0A2E9QSI3_9DELT (tRNA-specific 2-thiouridylase MnmA n=2 Tax=Deltaproteobacteria TaxID=28221 RepID=A0A2E9QSI3_9DELT)

HSP 1 Score: 409 bits (1050), Expect = 6.230e-137
Identity = 196/356 (55.06%), Postives = 250/356 (70.22%), Query Frame = 0

Query:  131 VAILLSGGVDSSVAMRLALESGARPHPFYLKIWLEDELAHMGECPWEEDIEYANAVCNQVGIKLQDIPFQRAYWDEVVSYTVQEAQKGRTPNPDVMCNSRIKFGAFYDRIGKYYKKVVTGHYAQTARDEMGRAELRLSPDELKDQTYFLAHLRQDQLATARFPVGKHTKEEVRMLAEHYDLPNKNRRDSQGICFLGKLKFDDFLGHHLGQKKGSLVEFETGQELGHHNGFWFFTLGQRRGVGLSGGPWYVVAKNVSQNIVYVSKDYNNVSKERNCFEFDSALWISGEWPAKLQHVGDKIKLRVKTRHGPRFHDATLTRLDDERGRVDLEGRDKGLAPGQFSAFYDIEGRCLGSGVI 486
            +A+L SGGVDSSVA+RL  E G     FYLKIWLEDELA +GECPWEED+ Y   +C Q  + L+ I  Q+ YWD VV YT+   Q GRTPNPD+ CN RIKFGAF+DRI   ++KV TGHYAQ       R+ L++S D  KDQTYFLAHL Q QL+ A FP+G +TK EVR LAE +DLPNK R+DSQGICFLG +K+ DF+ HHLG+KKG+L+E E+G++ G H GFWF+T+GQR+G+GLSGGPWYVV K+   NIVY+S++Y+   K+R+ F  +   W+SG        VG+   LRVK RHG   +DA +T   ++R ++ L   D+GLAPGQF+ FY  +  CLG   I
Sbjct:    3 IAMLASGGVDSSVALRLLKEQGHDVTAFYLKIWLEDELAFLGECPWEEDLGYVREICAQCDVPLEIISLQKEYWDRVVQYTIDTVQAGRTPNPDIHCNDRIKFGAFFDRIDDSFEKVATGHYAQVEHTPT-RSILKMSKDSWKDQTYFLAHLSQQQLSRALFPIGGYTKAEVRALAESFDLPNKKRKDSQGICFLGTIKYSDFIKHHLGEKKGALIELESGEKKGDHRGFWFYTIGQRQGLGLSGGPWYVVKKDPGNNIVYISRNYHANDKKRDTFYVEDFSWVSGF----TFEVGETRHLRVKVRHGELIYDALVTFETEDRAKIKLNETDQGLAPGQFAVFYHND-ECLGCATI 352          

BLAST of Gcaud8767.t1 vs. uniprot
Match: A0A523U0Y3_9BACT (tRNA-specific 2-thiouridylase MnmA n=1 Tax=Candidatus Dependentiae bacterium TaxID=2030827 RepID=A0A523U0Y3_9BACT)

HSP 1 Score: 408 bits (1048), Expect = 9.810e-137
Identity = 194/357 (54.34%), Postives = 262/357 (73.39%), Query Frame = 0

Query:  131 VAILLSGGVDSSVAMRLALESGARPHPFYLKIWLEDELAHMGECPWEEDIEYANAVCNQVGIKLQDIPFQRAYWDEVVSYTVQEAQKGRTPNPDVMCNSRIKFGAFYDRIGKYYKKVVTGHYAQTARDEMGRAELRLSPDELKDQTYFLAHLRQDQLATARFPVGKHTKEEVRMLAEHYDLPNKNRRDSQGICFLGKLKFDDFLGHHLGQKKGSLVEFETGQELGHHNGFWFFTLGQRRGVGLSGGPWYVVAKNVSQNIVYVSKDYNNVSKERNCFEFDSALWISGEWPAKLQHVGDKIKLRVKTRHGPRFHDATLTRLDDERGRVDLEGRDKGLAPGQFSAFYDIEGRCLGSGVIM 487
            +A+LLSGGVDSSVA++L  + G     FYLKIWLEDELA++G CPW+ED++YA  VC Q+ + L+ I  Q+ Y DE+VSYT+ E + GRTPNPDV+CNSRIKFG F   I   ++KV TGHYAQ  ++  G+ +L  SPD +KDQ+YFL+HL Q+QL  A FP+G  TK E+R LA+ +DLP K+R+DSQG+CFLGKLKF DF+ H+LG+K+G ++E ETG  +G HNGFWF+T+GQR+G+GL+GGPWYVV K+ ++NI+Y+S++Y +  K R+ FE D   W   + P + Q        +VK RHG + +D T+T  DD R  V L  RD+G+APGQF+ FYD + RC+GSGVI+
Sbjct:    3 IAVLLSGGVDSSVALKLLKDQGHVITAFYLKIWLEDELAYLGSCPWKEDLDYAQKVCKQLDVPLEIISLQKEYHDEIVSYTIAEIKAGRTPNPDVLCNSRIKFGMFLSAIDPSFEKVATGHYAQL-KEVDGQMQLLRSPDPIKDQSYFLSHLNQEQLKRALFPIGHLTKAELRKLADQFDLPTKDRKDSQGLCFLGKLKFRDFVKHYLGEKRGPMIEVETGMAVGEHNGFWFYTMGQRQGLGLAGGPWYVVDKDPTKNIIYISRNYYSPEKSRDAFEVDRCNWFI-DVPQQAQ------SFQVKMRHGAQRYDCTVTPKDDGRMAVKLHDRDQGIAPGQFAVFYD-DTRCVGSGVIV 350          

BLAST of Gcaud8767.t1 vs. uniprot
Match: K2DHK5_9BACT (tRNA-specific 2-thiouridylase MnmA n=2 Tax=Bacteria TaxID=2 RepID=K2DHK5_9BACT)

HSP 1 Score: 406 bits (1044), Expect = 4.530e-136
Identity = 195/359 (54.32%), Postives = 258/359 (71.87%), Query Frame = 0

Query:  131 VAILLSGGVDSSVAMRLALESGARPHPFYLKIWLEDELAHMGECPWEEDIEYANAVCNQVGIKLQDIPFQRAYWDEVVSYTVQEAQKGRTPNPDVMCNSRIKFGAFYDRIGKYYKKVVTGHYAQTARDEMGRAELRLSPDELKDQTYFLAHLRQDQLATARFPVGKHTKEEVRMLAEHYDLPNKNRRDSQGICFLGKLKFDDFLGHHLGQKKGSLVEFETGQELGHHNGFWFFTLGQRRGVGLSGGPWYVVAKNVSQNIVYVSKDYNNVSKERNCFEFDSALWISGEWPAKLQHVGDKIKLRVKTRHGPRFHDATLTRLDDERGRVDLEGRDKGLAPGQFSAFYDIEGRCLGSGVIMSD 489
            +A+L SGGVDSSVA+ L  E G     FYLKIWLEDEL+++GECPWEED+ Y  A+C+++ + LQ IP Q+ YWDEV+ YT+ E + GRTPNPDV+CN +IKFG FY+RI   ++KV TGHYA+    E G   L+ + D  KDQTYFLAHL Q QL+ A FP+G + K EVR LA+ Y+LPN +R+DSQGICFLG + FD+F+ HHLG+K+G LVEFETG+ LG H G+W++T+GQR+G+GLSGGPW+VV+K++  N V++S  Y+   K RNCF   +  WISGE P    H    + L+VK RHG + +DATL    +E  +V+L G D+GLAPGQF+ FY+ E  C G   I+ +
Sbjct:    3 IAVLASGGVDSSVALHLLKEQGHELTVFYLKIWLEDELSYLGECPWEEDLGYVRAICDRLDVPLQVIPLQKEYWDEVIRYTLVEVKAGRTPNPDVLCNQKIKFGKFYERIDASFEKVATGHYARVEEKE-GVFFLKTAKDTWKDQTYFLAHLSQSQLSRALFPIGDYLKSEVRELAKKYELPNADRKDSQGICFLGSIPFDEFVKHHLGEKQGELVEFETGKVLGCHQGYWYYTIGQRKGIGLSGGPWFVVSKDIVTNRVFISNHYHVEDKPRNCFRAHALHWISGE-PV---HTQAPLPLKVKIRHGQQRYDATLKWNGNEEVQVELVGSDQGLAPGQFAVFYEGE-ICRGIATIVGE 355          

BLAST of Gcaud8767.t1 vs. uniprot
Match: M2XAB2_GALSU (tRNA-5-taurinomethyluridine 2-sulfurtransferase n=1 Tax=Galdieria sulphuraria TaxID=130081 RepID=M2XAB2_GALSU)

HSP 1 Score: 413 bits (1062), Expect = 6.390e-136
Identity = 193/411 (46.96%), Postives = 285/411 (69.34%), Query Frame = 0

Query:    4 IPEDKLTASTRVSGCTSITHISIHLSDEGTISIRGYSDARISRGLVALFALGLHGCSTKDVLSLNLREVLHKSDLPGVIGSSRINSLGNILRAARKQVQMLTENASILRNDTAHILTGRWSERQGEDVAILLSGGVDSSVAMRLALESGARPHPFYLKIWLEDELAHMGECPWEEDIEYANAVCNQVGIKLQDIPFQRAYWDEVVSYTVQEAQKGRTPNPDVMCNSRIKFGAFYDRIGKYYKKVVTGHYAQTARD-EMGRAELRLSPDELKDQTYFLAHLRQDQLATARFPVGKHTKEEVRMLAEHYDLPNKNRRDSQGICFLGKLKFDDFLGHHLGQKKGSLVEFETGQELGHHNGFWFFTLGQRRGVGLSGGPWYVVAKNVSQNIVYVSKDYNNVSKERNCFEFDSALW 413
            +P ++  +  +V+GC S+ +I + L +E  + +RG++D+++++GL+ L   GL   + + V +L+   ++ K+ L  ++ SSR+  +  +LR  ++   +L +         +H  +   ++ + +++A+LLSGGVDSSVA+   LE G R  PFYL+IWLEDEL+H+G+CPWEED+ YANAVC   G+KL+ +  Q+ YW++VV YT+ EA+ GRTPNPDVMCNSRIKFG F D IGK Y+K+ +GHYA+  ++   G  +L +S D  KDQTYFLAHL Q QLA   FP+G  TKE+VR +A   +L  ++R+DSQGICFLG++KFDDFL HHLG + G LVE ETGQ +G H G+WF+T GQR+G+ L  GPWYV+ K+V  N V+VS++Y++  K+RNCF  ++  W
Sbjct:  116 LPVEERISKHKVAGCASVVYIKVTLRNE-KVFLRGFADSKVAKGLLFLLLSGLQDTAVQQVKTLSAEVIIQKAGLETILSSSRMGGIKGMLRYIQQHCNLLQKPEGAPSCSDSHQYS---TDSEKDEIAVLLSGGVDSSVALSRLLEQGYRVKPFYLRIWLEDELSHLGKCPWEEDLVYANAVCEHFGLKLEQVTLQKEYWEQVVEYTISEARAGRTPNPDVMCNSRIKFGVFLDSIGKTYQKIASGHYARIQKEPSTGLFQLMVSADAHKDQTYFLAHLLQRQLAKVVFPIGHLTKEQVRAIARELNLATQSRKDSQGICFLGQIKFDDFLRHHLGTRPGKLVEAETGQVVGEHCGYWFYTPGQRKGLRLPNGPWYVLTKDVESNTVFVSRNYDSPEKQRNCFIVENVHW 522