Gcaud8403.t1 (polypeptide) Gracilaria caudata M_176_S67 male

BLAST of Gcaud8403.t1 vs. uniprot
Match: A0A2V3IML8_9FLOR (Midasin n=1 Tax=Gracilariopsis chorda TaxID=448386 RepID=A0A2V3IML8_9FLOR)

HSP 1 Score: 1635 bits (4234), Expect = 0.000e+0
Identity = 1047/2567 (40.79%), Postives = 1469/2567 (57.23%), Query Frame = 0

Query:  308 ESLVAIMRLLWLLDSIGEDMKNNDTEAHIENNEQDLFLLALLRELNECCEPERSTVFAEVKVDSNPVKAIVGFLRAPQRYLFWNLEPAYRQAAATSGVLALNSRVCESVRDASFKVLFLALMSALRLDQVRPEAQKIIQDLEHVSFQDKLASLVSLVERHDSDENGDADSKRKPDEKLALSSVVQAFK----VKSAPERRDRENLRYMGTAWISVGVYRLQSYFSICDEVRGNDPSTLTEKRVVLSLETKVRLEARRKAYELLQMSRFGGDEFSSSIPAEEMSNQLKVEDDKLATGSSCHVYRDPSKIGFESFAGVVQAVRLNIVSLIEKNGFLSRAKQFREVQLLR----LIPEARHLVEICKTTSESLGFHGSHSHFRDLSLRLRLGIEEIHHGLQWFLRATQIIGVDENPVLHKKVKVLLDLCLLPRATFRNASELPDAFSLSAEIAPSGKALMTTARFLLENEYLDDDKAFSHLSDFFSRVVKIWKASVERSEIEAQRRESLHVIRQSSQRNELDATAFLEVSEQQEVEDFVSTFNPIGNDLEDELLD--------------------------------------------------------------------------------------------CIEEIQRL------------------FF--------------------------------KENGNHPVLDGIMVAIDSVARVSKSSTPLSIVVIGLENVLRKADEWNRLFATGETKLRAELVEVSRLVSRWRGVEMRSWPLLLAARKREFETQADKWVFLIYDAVINETLSAGMHDNEQVNSALSTIDRFLRSSPSGEFCRRLAIVQSISHHLLSLNDARRDLAQFIGNSLRGICRFYHLFKDHLEQKLSQRYETVLQELKEFTNIVSYNPQEELGSAVEMSKGRDKNLDYFRLKALSDKTRRRVHKLCLKVDGILRVPFYEHMMDFTAGFGFDHPIILNGASKHDPVRPLGQDILTKTSRGIACDERDV----------LDSKHRSSILLPAALKMLSRFDTLRTRLVTYGREIQSSSRTECEAASAFSSKLRTMIRARVSKLREMRLGSDIQPKRRALIDLLKGLSRAGLSPFERPHANSMLRWLSTSDPSSCEVSNKIANHLYFSGIHQYQTLRNVSDSSTRNNDISAEEAGRIRAFCRDLLDKSAMERTELNKCSAIVHCLSKVADALTKGHQDILSTSRENPAFFGCFRDILFRLRKAKSTLQQISKLMSEAQRNMSGDADFVQGQGTLDEVLCFYRKVGKKSLSELQIILNTCCDKIGNQPKAFSLMDIDHVEYQELAYFDGSYKEWCETIRAYLSSLKETLHVGLSRAREVSEHNLVTEILSPLLSLVEDGISEES----PLPSSPAQVASALYNSLLSNAEELVHSILISSQKAMAWNGVRELLGENKSAAPPLGKDETELFPEGVIRRGHDGTVNLGSYLLLTRISSHFDANSRFLQSLIPLRDVLAWKETKELIGIQNALGSILKEYLRSMLMPCLEKAIVHHHENLALLRTLTNLFIGICKDGFCRPDLEEQTEGMMETEVKSGTGFGDVGNGDMSGAQDVSEAIEDEEQLLGLRDERDKDLRDENENVRNEDGFDMKTDFEGTLEDVDSEGVQDMEGDGEAEKQMGQEHGKGENLLNEKLWDSENETNKEQASELDSKQDEEESESQGNGKQGEWAVGDRRDRSHSKEMNEQNREEEKSELDASRVXXXXXXNAEEELGSYEEPEDLEKDASRDREHGDHRENGEQENCDTHSEDIQESGTEKQKKHGEEDHGMTDGYNS--DFARGQEPDP-----------CLPESDQPEVGEAESNCGSPMVGVGDEAEVLEY-EGMDEDQTGD----FHEDCQLSGLEEEADMGNEKTNPEDKLEHVKKDVSPDLAKTNEEEEAKSISSAQDDIKSDGGEHCQETEGDSDVLNGADADAKQPVSEKEHRS--MNILPGRSTTGQADKEIEGSTEPFAHQGQGTLGFGSNMNDADGERNDIADRSSHDHQPGPGNQINKQNGEEEGRKLESSMKQD-EQLEKGSSSMNTNPLRLSDPEHLIKEWNECLRALRDD-DEQL--PKQTEGENDADAWQYETIDDPKENPQHSLGAATQKQQRPLPDERVAELD----SESIRHKE----AEPKPKLREAMSTQDRQKSHFRQEQSSSPQNPNESLGDLVHGDVEKPHPVRSAIDRERERNRIASAHIGQSESSIFETRGKPGCDEDERNEEPTPVTSQVLGWRMENLDHAQAKELWHALESKVASEAATLCEQMRLVLEPTKASRMGGGYRTGKRLNMRKVIEYVASDFRKDRIWLRRVKPDKRSYDVLIAIDDSASMLESEAGVMATESLALVISALSKLEVGRIAVASFGSTANLIRHFEEPLPINTESGAKLLQHFTFSQTETNIVNLLSFIHSQMGSTNNESTVEHISLALVISDGRLSHREEVRRQLRILKESNVLVAFFILDRGTVEENSIYNVQRVEYEANGKMSVRPYMHDFPIDFYAVVQNMSSLPGTLADALRQWIEITNY 2678
            ESL+ + R  W+L+ +  + KN+ +   ++  E DL LL++LRE N                          F+R   R                           +   D + ++     ++ L+    +PE   I+  L+ V  Q K + L SL +    +  G   S+    E LA+S   +       +  +   RD   L Y   AWI++G YR+  Y  I   + GNDPS   + + V+S E+ ++ EAR KA+  + ++R GGD   S  P E +  Q++   + L    S ++YR+P + GFESF  +V  V+ +++ +I ++GF+ RA    EV  +R    L+ EA HL+++C+   ++L   GSHSHFRD+SL LRLG+EE+  GL  ++ A++ I   +      K ++L DLCLLPRA  + A+E+ +A S  A+IAP+G+AL+ TA+FLL++ Y+  D +  + S F S +V +WKAS  R E E   RESLH++RQ+SQR E+DA A L  S + E EDF  TFNPI  ++E+ L+                                                                                             C E I R+                  F+                                 EN +HPVL  I+ AID VARV+K+STPLSIVV+GLENVLRKADEWNRLF + ETKL AELV VS+LV RWR +EM SWPL+L +RK+ FE +A KW+FL+YDAV+ E+LS    +     S +ST+D+FLRSSPSGEFCRRL IV S+SHHLLSL     D+ + IG  LRG+CRFY LF++HL+++LS+ YE+VLQEL+EFT+IVSYNP+ +LGS++EMSK  +K+LDYFRLKA S+KTRRR+HKLCLKVD +LR PFY HM D T+  G      L G     P+    ++  +  S  IAC E  +          L+S   +++   +    +S+F+TL+ +L  YG+  + S    CE AS F+ +LR +IR RV +LR++  G DIQPKRRAL+DLL+GL + GLSPFER + N  L W+   DP+S EV N  AN LYF+G+H+YQTLRN+SDSS R+NDI++ EA RIRAFC DLLDK+ +ER ELN+C  I+  L  +A++L     +  ST   +   F  + +++ RLRKAKS +++I  ++ +A        + +    T+ + + FY  +G+K L EL  +L+   + I N+PK    +D+    YQE+ YFDG +++WCE ++ YL SL  TL   L +A  VS  N+VT++L+PLL+LV++G+S+E+     L S P++   AL  SL  N EE V +ILIS+Q  MAW      +        P  +   E+FP  V++ GH   V LGS L L+R+ S  + N+  L  LI  R  L+  E  +++  Q ALG I+KEYL SML+PC  KA   H E+LALLRTLT+LFIG+CKDGFCRP  E++     E E  +G GFGDVG+GD++GAQDVSE IEDEEQLLGLR+E  K   ++     N DG++M+ DFEG LE++D+    D E   +AEKQMG E+  GEN LNEKLWD           E   K + +++    +   G WA G+RR+  +SK    Q +E                   +EE    EEP+D  +  + ++E G     G++ +   H E+++   T+K +     +    +G  S  D   G++P+               E    +  EAE     PM+G   +++ +E+ E  ++ ++GD      ED QL   E                                  E +   SA++D         QE+E +++V  G  ++ +Q V  +   +  M+ILPG STT Q D++   +   FA+QG G+L F     +  G++++ +++       G  +Q N     +     ESS KQ  E+ ++G S+  TNPLR+SD E L+K+W+EC+  +RD  DE L  P+ +  E+ AD W++ET     E P+H+LG A+ +QQRPLP +   E      S+S + K+    A+ +P +     T+++  SH       SP   + +L DL +G +E PHP RSA ++ ++   +++ H  Q    +   +     D  E + EP P   Q+   R+E+LDHA+AK LW ALESKV+++A TLCEQ+RLVLEPTKASRMGG YRTGKRLNM+KVIEYVASDFR+DRIWLRRVKPDKR+YDVLIAIDDSASM+ES AG+MATESLALV +ALSKLEVGR+AVASFG+ A L+R+FEEPLPI+TESGAKLL+HFTFSQTETNIVNLLSF+H+ M + N  S ++HISLA +ISDGRLS REEVRR LR L+ESNVLVAF ILD+   E++SIYNV+RVEY++ GKM V PYM DFPIDFYAV+Q++ SLP T+ADALRQWIE T+Y
Sbjct: 2967 ESLLVMQRRSWILECLETEEKNSPSSTDLDVVEGDLLLLSVLRERN-------------------------AFVRGLSR---------------------------DEDTDTATQLGHDPFLTTLQRKDKKPE---ILSTLDAVPMQAKCSYLASLWQDGAFNSIGSKFSETTDYENLAVSRTAKFLHESPVIDGSGSHRD---LLYGSMAWIAIGAYRIICYVDIIQRMNGNDPSAFAQMKTVVSFESIMKFEARLKAFTSVHINRTGGDCLQSCRPVEMICEQIRTARESLKQSRSYYIYRNPEEAGFESFVDLVGKVKASVIDVISQSGFMGRA----EVNGIRADPGLVQEASHLIQVCREIGKALSICGSHSHFRDISLSLRLGLEELQTGLSAYINASEQIEHSKCDWFLTKRQILSDLCLLPRAACKAATEISEAISSCAKIAPTGEALLATAQFLLQSHYIPGDPSPQY-SSFLSGLVSVWKASKLRHEQEDAERESLHLLRQASQRYEIDAEAALGTSTELEQEDFCDTFNPISEEVEEGLIGISQSCEDSEPPKMKANTHAELENGFLTIDSMRFWDLHQSIFPGISFSSSNGLSIQHDVETIISFAKDLSSYVQESRFFVIGSNGLGGIWPFLCSESITRMLRGMKSVPKPQNLRTRYNFYTDSNIAEVRTASTLLLELRKAVGNIQSAVSKGNENEDHPVLSEIVSAIDRVARVTKTSTPLSIVVMGLENVLRKADEWNRLFGSRETKLTAELVAVSKLVLRWRALEMNSWPLILESRKQSFEQKATKWIFLLYDAVVTESLSQKFLEESHCRSVISTVDQFLRSSPSGEFCRRLEIVNSVSHHLLSLRGVG-DVVKHIGRGLRGLCRFYKLFEEHLDKELSRGYESVLQELREFTSIVSYNPEADLGSSMEMSKQNNKHLDYFRLKAASEKTRRRMHKLCLKVDSVLRDPFYRHMTDMTSSIG------LAGIDFGPPMTE--RESCSTQSHYIACTESILKAMLVASPAPLNSGSLTALSQVSGDSYISKFETLQRKLFLYGQNAKDSKHGSCEEASKFAFRLRCLIRERVKRLRQVCAGKDIQPKRRALVDLLRGLHKVGLSPFERRYENQSLEWICAVDPTSDEVFNATANDLYFAGVHRYQTLRNLSDSSVRSNDINSGEASRIRAFCHDLLDKACIERKELNQCYDIIQSLHCLANSLVTTRGNTSSTFHVDNDLFNDYCELILRLRKAKSDVKKIIVIIEDAVSREVDVQELMCSNETVRQTMKFYNDIGQKPLRELHTLLSLTFEAIENEPKDVGFVDLKGKRYQEMMYFDGRFQDWCEAVQCYLRSLCNTLKEVLQKAGNVSNDNIVTQLLAPLLALVQEGVSKENGEKRALFSQPSETCKALCESLHKNIEEFVRTILISAQNVMAWCAAGSTMENELEKELPTEESALEVFPRLVLKHGHQKIVRLGSDLGLSRLHSCAEKNAEILNKLISSRFCLSRTELNDIVVQQRALGGIMKEYLNSMLLPCTRKASTQHCEDLALLRTLTSLFIGLCKDGFCRPPDEDEVNDSREVETSAGAGFGDVGDGDLTGAQDVSEQIEDEEQLLGLRNETSKTAEEQKNEHGNRDGYEMQNDFEGDLEELDTGENGDSEELSDAEKQMGHENSVGENTLNEKLWDENEAEGINNPDESQHKGENDDNLENPSSNSGGWAAGERREEQNSKLKTSQEQE----------------VGTDEEHSDSEEPKDDNESTASNQEPG---LGGQENDVGQHLENMK---TDKPQDLDSRNKAGEEGLESTHDVLGGEDPEANECDVRDDVSDAQAEERDAQENEAEDPSNEPMLGR--DSDAIEWDENHEKKESGDGLEGIPEDPQLPEGEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEPQPERSAREDNVETTPSEPQESE-NAEV--GTTSEKRQNVEPETTATDAMSILPGESTTEQPDQDRSTAWAAFANQGSGSLEFRGGRTETVGDQHNRSEQRDDKLHSGHTDQENATIEGDGQTMYESSRKQKGEENQRGLSTEGTNPLRISDSEDLVKKWDECISVIRDQADESLIPPRNSHDEDGADVWEFETNKTADEAPRHALGTASAEQQRPLPQDEGPESSRGEKSKSEQEKDMGGLADGQPHI-----TEEKTLSHI---PPLSPTRTDGNLVDLHNGQLEIPHPARSATNQTQQYTALSN-HSPQRGEKLSYGKSTMEVDSCEGSSEPKPK-HQINRRRIEDLDHAEAKGLWQALESKVSTQATTLCEQLRLVLEPTKASRMGGAYRTGKRLNMKKVIEYVASDFRRDRIWLRRVKPDKRTYDVLIAIDDSASMVESNAGIMATESLALVTTALSKLEVGRVAVASFGADAKLVRNFEEPLPISTESGAKLLRHFTFSQTETNIVNLLSFVHTHMAAANCTSELDHISLAFIISDGRLSDREEVRRHLRRLRESNVLVAFLILDQTEHEDSSIYNVKRVEYDSAGKMRVNPYMQDFPIDFYAVIQDVQSLPTTVADALRQWIEATSY 5424          

BLAST of Gcaud8403.t1 vs. uniprot
Match: R7QIZ4_CHOCR (Midasin n=1 Tax=Chondrus crispus TaxID=2769 RepID=R7QIZ4_CHOCR)

HSP 1 Score: 758 bits (1957), Expect = 1.940e-220
Identity = 627/1944 (32.25%), Postives = 963/1944 (49.54%), Query Frame = 0

Query:  853 IEEIQRLFFKENGNHPVLDGIMVAIDSVARVSKSSTPLSIVVIGLENVLRKADEWNRLFATGETKLRAELVEVSRLVSRWRGVEMRSWPLLLAARKREFETQADKWVFLIYDAVINET--LSAGMHDNEQVNSALSTIDRFLRSSPSGEFCRRLAIVQSISHHLLSLNDARRDLAQFIGNSLRGICRFYHLFKDHLEQKLSQRYETVLQELKEFTNIVSYNPQEELGSAVEMSKGRDKNLDYFRLKALSDKTRRRVHKLCLKVDGILRVPFYEHMMDFTAGFGFD----HPIILNGASKH---DPVRPLGQDILTKTSRGIACD---ERDVLDSKHR----SSILLPAALKMLSRFDTLRTRLVTYGREIQSSSRTECEAASAFSSKLRTMIRARVSKLREMRLGSDIQPKRRALIDLLKGLSRAGLSPFERPHANSMLR---WLSTSDPSSCEVSNKIANHLYFSGIHQYQTLRNVSDSSTRNNDISAEEAGRIRAFCRDLLDKSAMERTELNKCSAIVHCLSKVADALTKGHQDILSTSRENPAFFGCFRDILFRLRKAKSTLQQISKL-----------------MSEAQRNMSGDADFVQGQGTLDEVLCFYRKVGKKSLSELQIILNTCCDKIGNQPKAFSLMDIDHVEYQELAYFDGSYKEWCETIRAYLSSLKETLHVGLSRAREVSEHNLVTEILSPLLSLVEDGISEESPL----PSSPAQVASALYNSLLSNAEELVHSILISSQKAMAWNGVRELLGENKSAAPPLGKDETELFPEGVIRRGHDGTVNLGSYLLLT-RISSHFDANSRFLQSLIPLRDVL----AWKETKELIG-----IQNALGSILKEYLRSMLMPCLEKAIVHHHENLALLRTLTNLFIGICKDGFCRPDLE--EQTEGMMETEVKSGTGFGDVGNGDMSGAQDVSEAIEDEEQLLGLRDERDKDLRDENENVRNEDGFDMKTDFEGTLEDVDSEGVQDMEGDGEA---EKQMGQEHGKGENLLNEKLWD-------------SENETNKE-QASELDSKQDEEESESQGNGKQGEWAVGDRRDRSHSKEMNEQNREEEKSELDASRVXXXXXXNAEEELGSYEEPEDLEKDASRDREHGDHRENGEQ-------------------ENCDTHSEDIQESGTEKQKKHGEEDHGMTDGYNSDFARGQEPDPCLPESDQPEVGEAESNCGSPMVGVGD-EAEVLEYEGMDEDQTGDFHEDCQLSGLEEEADMGNEKTNPEDKLEHVKKDVSPDLAKTNEEEEAKSISSAQDDIKSDGGEHCQETEGDSDVLNGADADAKQPVSEKEHRSMNI-LPGRSTTGQADKE-IEGSTEPFAHQGQGTLGFGSNMNDADGERNDIADRSSHDHQPGPGNQINKQNGEEEGRKLESSMKQDEQLEKGSSSMNTNPLRLSDPEHLIKEWNECLR-ALRDDDEQLPKQTEGENDADAWQYETIDDPKENPQHSLGAATQKQQRPLPD---------------ERVAELDSESIRHKEAEPKPKLREAMSTQDRQKSHFRQEQSSSPQNPNESLGDLVHGDVEKPHPVRSAIDR---ERERNRIASAHIGQSESSIFETRGKPGCDE---DERNEEPTPVTSQVL------GWRMENLDHAQAKELWHALESKVASEAATLCEQMRLVLEPTKASRMGGGYRTGKRLNMRKVIEYVASDFRKDRIWLRRVKPDKRSYDVLIAIDDSASMLESEAGVMATESLALVISALSKLEVGRIAVASFGSTANLIRHFEEPLPINTESGAKLLQHFTFSQTETNIVNLLSFIHSQMGSTNNESTVEHISLALVISDGRLSHREEVRRQLRILKESNVLVAFFILDRGTVEENSIYNVQRVEYEANGKMSVRPYMHDFPIDFYAVVQNMSSLPGTLADALRQWIEITN 2677
            +   Q  FF E+G HPVL+ I  AI+ V       TPLS +V+G+E VLRKADEW RLFAT  T L+ E+  +S +  +WR  E  SW LL+  R R  E++A  W   +YDA+I E   +   + D E   + ++T+D+F+RS+PSGEF  RL ++ S++ H+LS+      L   IG  +RGI RFY+L    + ++LS+  + V  +L EF+ + S+ P   LG++ +M++  +K L+Y+RLK  + +T+R++HKLCL++D +LRVP Y+++       GF        +  GA+     D  +   Q ILT  + G+  D    R+  D+  R    S + L     +LS+     TRL  +G  ++ S  ++C++   F    R+ IR+R  +LR  +    IQ K+RAL+DLL+ L   GLSPFE   + S ++   WLS+ DPS     NKI+N L++ G  Q + LR+VSDS TRN DI+++EA + +A C  L D +  +R  LN  +  V  L+ ++  L +  +D+    R    +    R  L R       +Q   KL                 + + Q  + GD  F     +L +V      + +  +  L ++L    D +    + F                  +Y    E +   ++  K  L   L RAR +SE N   + L P+ + +   + E        P  P      L N +   +   +  +L+ +Q  +AW  V  +  E +S        E      G       GT+      +L+ R SS      + L  L+ L++ L     + +    +G     +Q  +G ++  Y+ +M++P L K    H  +LALLRT++++FIG+C +GFCRPD    E TEG  E   K+G GFG++ +GD+  A++VSE IEDE QL+GL+ +R ++   E  N   E GF+M  DFEG LED + E  +D + D  A   EK +  E G GE++++E+LW+             S+ + NKE Q +EL ++  +E S+ Q +  +      D            ++ EE + + D            +EE G+    +   +DA+ ++ +GD  + G Q                   E         QE G+    +H EE     D   +    G + DP    S   E    E+  G P  G    E    E + +  D            G + E++  N +  P+ + + V+++ + + ++   EE+     +     +S GG            L  ++ +  Q  SE+  +S  +   G       D E I G+++                    GER   + RS  + +P     + K +G+++ +     M  D    K S   ++NPLR++  + LI  WN+ L  A R  +E+L ++ +      A +Y+  D+   +   +LGAAT++Q +PLPD               ++  ELD +      A+ +P     +S    + ++ R     S Q  +E      H D    HP R A +    +  R+  A A +   +    ETR +    +   DE++       S VL                        L       A++LCE +RLVLEPT AS++GGGYRTGKRLN+RKVIE+VASDFRKDRIWLRRV+PDKRSYDVL+AIDDS SM ES AG MA ESLALV SAL KLE+GR+AVASFG+ A +++ F+EP+ I+   G +LLQHF FSQ ETNI  LL F+   +       +VE I L  VISDGRLS RE+++R+LR L+++NVLVAF I+D+   ++ SIY+V+RVEY+++GK+ V PYMHDFPI+FYAVVQ++  LP  LADALRQW+EIT+
Sbjct: 3430 VSRTQNKFFGESGGHPVLEEISAAIERVTNTCHVLTPLSKMVVGVEGVLRKADEWQRLFATATTTLKDEVRALSHVAVKWRRRERASWKLLMQNRVRAMESRASIWFLYLYDALIQEASLIDQSLKD-EACQNIITTVDQFMRSAPSGEFACRLEMLISLAAHILSVGSKGNKLLHTIGLCIRGIGRFYYLNAPAISEELSKEKKLVEAKLAEFSKLTSWEPGLGLGASQKMARVPEKQLEYYRLKEDAQRTKRKLHKLCLEMDSVLRVPVYKYLTQEVTKLGFGTLLKEEAVCGGANPKKGIDHGKSTEQRILTSVA-GLVQDMLVSRETRDNVFRGDALSDVWLSDGNPLLSKLPLFETRLNQFGMIMEKSQCSDCDSGMEFCRDARSTIRSRALQLRRSK-NPSIQLKKRALVDLLQALRNVGLSPFENRKSESTVKSFQWLSSPDPSESPSFNKISNDLFYDGAQQLRRLRDVSDSRTRNTDITSDEAQKSQAMCTHLFDMACAQRESLNSLTQDVDRLATMSSHLQEIRKDLFD--RVGRVWNSAARSHLLRTINKLMKIQIDLKLAHSSVTCALSASPKSDSLRQEQGPLFGDGQFSDTSDSLRQV----STILENGIQILDLVLQA--DDVAKLERGFKKCSKP-----------PTYASCYERLVGNITDSKVRLDEELLRARALSEQNFAVQTLMPVTTFLHVALEELGKKIELQPGQPTMNPVDLKNGIEKLSVSAIEKVLVGAQNTLAWGKV-SIKNEAESKIVTASSFENGKLAGGA----RQGTLRCAHAEILSHRQSSKIPDLMKILGELLELQEQLEVCMGYDDDLHRVGKRSAMLQGCVGKLVSTYIGTMVLPSLSKMGSVHCHSLALLRTVSSVFIGVCTEGFCRPDETNPESTEGE-EMMDKTGAGFGNIEDGDIREAKNVSEDIEDEGQLIGLQSDRREEAEKEPNNTDQEPGFEMTNDFEGDLEDFEPE--EDDKTDQNAQDPEKDLSAESGHGEDVIDERLWEQGDDPLGPSNGESSQADANKENQQTELKARSGDEASDKQASASK---PSDDGXXXXXXXXXXRKDGEEYQEDGDTP---------DDEEAGNKNPIQGTGEDANEEKTNGDSEQEGSQSRLPDQLNLKGNEGDADGMEEXXXXXXXXQELGSASGNEHEEELTSDQDEQTTGVEEGIQ-DPLTDSSPLKEQQNEET--GIPEAGAQSVEGSDCEMDNLPLDXXXXXXXXGMEKGPDMESNTPNVEEQPDKETQEVEQNGATEGSRAGHEEKPSCEDALSLPGRSIGG------------LEQSNNNPAQFKSEETEKSQQVPASGSGQLAPTDDEFIHGNSQEH------------------GERQHPSTRS--EPEPPTMGSVGK-SGQDQSQ---HDMTDDLHASKDSELPDSNPLRVTANDDLISSWNKYLEVADRKREEKLQQEADTNRQDGALEYQEEDESDPDGLVALGAATKEQHQPLPDVAPNDTMEDIDDVKDDKPGELDKKLPSSSNAKSRPPQ---ISMAPAETTNARNIPPRSQQT-DEDEHSGSHCDEPTTHPARIAAESSTPDSSRSNGARAQLKDRK----ETRARMDISDNHDDEQDASKQHTESNVLEEANSXXXXXXXXXXXXXXXXXXKLVQSTTGGASSLCELLRLVLEPTIASKLGGGYRTGKRLNIRKVIEFVASDFRKDRIWLRRVRPDKRSYDVLLAIDDSESMSESRAGPMALESLALVTSALGKLEIGRLAVASFGAAALMVKDFDEPISISDVRGGQLLQHFCFSQRETNICELLQFMQDTLVGVKGPESVEQIKLVFVISDGRLSAREDIKRRLRHLRDANVLVAFLIIDKSGKDDTSIYDVRRVEYDSSGKIEVMPYMHDFPIEFYAVVQDVQRLPTILADALRQWVEITS 5284          

BLAST of Gcaud8403.t1 vs. uniprot
Match: A0A015JSW3_RHIIW (Midasin n=8 Tax=Rhizophagus irregularis TaxID=588596 RepID=A0A015JSW3_RHIIW)

HSP 1 Score: 328 bits (842), Expect = 3.790e-86
Identity = 454/1899 (23.91%), Postives = 826/1899 (43.50%), Query Frame = 0

Query:  887 STPLSIVVIGLENVLRKADEWNRLFATGETKLRAELVEVSRLVSRWRGVEMRSWPLLLAARKREFETQADKWVFLIYDAVINETLSA----------GMHDNEQVNSALSTIDRFLRSSPSGEFCRRLAIVQSISHHLL--------SLNDARRDLAQFIGNSLRGICRFYHLFKDHLEQKLSQRYETVLQELKEFTNIVSYNPQEELGSAVEMSKGRDKNLDYFRLKALSDKTRRRVHKLCLKVDGILRVPFYEHMMDFTAGFGFD-HP----------IILNGASKHDPVRPLGQDILTKTS---RGIACDERDV--------LDSKHRSSILLPAALKMLSRFDTLRTRLVTYGREIQSSSRTECEAASAFSSKLRTMIRAR--VSKLREM-RLG------SDIQPKRRALIDLLKGLSRAGL-------SPFERPHANSMLRWLSTSDPSSCEVSNKIANHLYFSGIHQYQTLRNVSDSSTRNNDISAEEAGRIRAFCRDLLDKSAMERTELN-------KCSAIVHCLSKVADALTKGHQDILSTSRENPAFFGCFRDILFRLRKAKSTLQQISKLMSEAQRNMSGDADFVQGQGTLDEVLCFYRKVGKKSLSELQIILNTCCDKIGNQPKAFSLMDIDHVEYQELAYFDGSYKEWCETIRAYLSSLKETLHV--GLSR-AREVSEH-NLVTEILSPLLSLVEDGI-----SEESPLPSSPAQVASALYNSLLSNAEELVHSILISSQKAMAWNGVRELLGENKSAAPPLGKDETELFPEGVIRRGHDGTVNLGSYLLLTRISSHFDANSRFLQSLIPLRDVLAWKETKELIGIQNALGSILKE---YLRS--MLMPCLEKAIVHHHENLALLR-TLTNLFIGICKDGFCRPDLEEQTEGMMETEVKS-GTGFGDVGNGDMSGAQDVSEAIEDEEQLLGLRDE-RDKDLRDENENVRNEDGFDMKTDFEGTLEDVDSEGVQDMEGDG---EAEKQMGQEHGKGENLLNEKLWDSENETNKEQASELDSKQDEEESESQGNGKQGEWAVGDRRDRSHSKEMNEQNREEEKSELDASRVXXXXXXNAEEELGSYEEPEDLEKDASRDREHGDHRENGEQENCDTHSEDIQESGTEKQKKHGEEDHGMTD--GYNSDFARGQEPDPCLPESDQPEVGEAESNCGSPMVGVGDEA-EVLEYEGMDEDQTGDFHEDCQLSGLE------EEADMGNEKTN----PEDKLEHVKKDVSPD---LAKTNEEEEAKSISSAQDDIKSDG-GEHCQETEGDSDVLNGADADA-----KQPVSEKEHRSMNILPGRSTTGQADKEIEGSTEPFAHQGQGTLGFGSNMNDADGERNDIADRSSHDHQPGPGNQINKQNGEEEGRKLESSMKQDEQLEKGSSSMNTNPLRLSDPEHLIKEWNECLRALRDDDEQLPKQTEGENDADAWQYETIDDPKENPQHSLGAATQKQQRPLPDERVAELDSESIRHKEAEPKPKLREAMSTQDRQKSHFRQEQSSSPQNPNESLGDLVHGDVEKPHPVRSAIDRERERNRIASAHIGQSESSIFETRGKPGCDEDERNEEPTPVTSQVLGWRMENLDHAQAKELWHALESKVASEAATLCEQMRLVLEPTKASRMGGGYRTGKRLNMRKVIEYVASDFRKDRIWLRRVKPDKRSYDVLIAIDDSASMLESEAGVMATESLALVISALSKLEVGRIAVASFGSTANLIRHFEEPLPINTESGAKLLQHFTFSQTETNIVNLL--SFIHSQMGSTNNESTVEHISLALVISDGRLSHREEVRRQLRILKESNVLVAFFILDRGTVEENSIYNVQRVEYEA-NGKMSVRP--YMHDFPIDFYAVVQNMSSLPGTLADALRQWIEI 2675
            ++P++  + GLE +L+K ++W   +A  E  ++    +++ L+ +WR +E+  WP LL A+ +  E  A KW F +Y+ +++   S             +  + ++  + T+D+FL+SS  G+F  RL ++ +   +L         + +D    L     ++L  + ++Y  F DH    L +  + + +ELK+F  I S+                 K+++   LK  + KT R+++K   K   IL  P  + + D+        HP           + N  S  +PV P+G+ +  + S   +     ER +        L S  +  I     L      DT  T ++   ++ Q  +       +    K + +I+ +  V  LRE+ RLG       +I  +++ ++  +  L R  L       + FE     S +R     D      + K A+  Y+  I +   LRNV+ S ++  D++++E  +   F  DLL     ER  +        +   ++   S++  +    H    S   E  +F       ++     K  L  ++ L+  +          +Q Q      L  Y ++G K        L+   DKI    K     D  H+  +E+   + +  +     +  ++ +K+ + +  GL + AR+ S      + IL+P+   +++ I     +E      S     + +  ++     +L+ ++L++ Q             +  SA P   +D  +  P+G IR+ H+  +NLG +L +  +   F       + LI L D     E    +  QN + ++L+    +++   +++     + ++HH++L  L   L N F  +   G+C P +E   + + + E  + GTG G+       G +DVSE IEDEEQ+LG ++E +D D  ++   ++ + G +M+ DFEGT+EDV                 E++MGQ      + ++EK+W              DS  D EES    N KQ   A G         E +   +E++++ +  S+       N E ++    + + +E +   D +   + E    +  D  + DI ++ T +      ED  + D  G  ++        P   + D+ EV +A+    +   G  + A + +E + +D+D   +  +D    G+E      E +D   ++++    P+++  H K  +S D   L   N E E +  SS Q+   SD   E+    +G+S   N A + +     +Q + E E++  N    R  +                             DA+  R+ + D                    +  R+L++ +K  E   +  +++  N +   D         +    L +DD+    QT G    D  Q   ID+ +++  +    A ++           E+D ++ +      K ++ +A+ T D   +H          N     G ++   + K    +   D +     I S+H      +  E        E  R E    +  ++  WR    D  +A+ELW   E+     A  LCEQ+RL+LEPT A+++ G YRTGKRLNM+K+I Y+ASDF+KD+IWLRR KP KR Y V+IA+DDS SM E+ +  +A E+LAL+  ALS+LEVG +++ SFG    L+  F+ P   ++E+GA++LQ FTF Q +T + +L+  S    +    N     E   L L+ISDG     E ++  +R   E+ +++ F I+D    ++ SI ++  V+Y++ NG+M++    Y+  FP D+Y V++++++L  TLADALRQ+  I
Sbjct: 3759 NSPIAKFLTGLEILLQKTEDWEA-YANREVSIKLHREKITNLIVQWRQLELTCWPNLLTAQDKYHERTAYKWWFHLYNCIVHPVDSLCIKVATKERIDQNFKDHISEIVRTLDQFLQSSKIGDFKARLDLIHAFYVYLCMKDYYRVRNASDQSASLYSKTADALLNVYKYYGQFLDHNTNTLKELRKPIEKELKQFVKIASW-----------------KDVNIHALKQSAQKTHRQLNKCVRKYKDILNKPIMDVITDYQINMTTTIHPREKDVIEQAMTLENWISNCEPVEPIGRILELQNSLNRQNFPVPERFLNIRNTLKKLRSYCQQDIYTKINLTYNRALDTFTTEIILRIKKFQDETPHLMNEENKNIIKNQKLIKKKTMVDLLRELKRLGLCYFPNKNIIEQQQNIVGYVLQLRRVELQNSLPMINDFEILKVPSSVR----DDVRESISTWKKADEYYYKIIARMIHLRNVAASYSK--DLTSQEVQKGLGFSEDLLYLLIQERKNIQALDKQYVRLLGVLIQFSRIYSSHNAQHS--YSKVCEYLSFCSIDERTVWNF---KEILDDLNSLLFHSCVIFD-----IQKQNEQSNYLSHYEEIGTK--------LHDWLDKISTVRK-----DFVHLFDREILLPEYTIGKKALITKDIINLVKDNIVLIDGLYQFARDYSSRIKCFSHILAPICDYIKNCIGNLYLTETVHNEMSLNSQTTTVLGNIAKKVNDLIDAVLVAIQDL-----------QKSSAEPTKDEDIDDDIPDGYIRQEHEHILNLGKHLRIPLVLERFIILH---EQLINLTD----DERFNNLDCQNLISNLLQRIFPFIQQYYLVVQYYLNSFIYHHKSLCKLTYVLCNSFTTVFMKGYCMPQMESGDDEIGDIEENAQGTGIGEG-----EGTKDVSEEIEDEEQVLGNQNEMKDHDSANDRLKLKKDKGIEMENDFEGTMEDVXXXXXXXXXXXXXXENIEQEMGQFDDNDPDAVDEKMWGD------------DSADDVEESRKTTNTKQESGAQG---------ETDIVAKEDQETTIQDSQEKNIE--NGEMDVEGDNQRDSMETEYQSDDDDNINEEYDNYDKSDHFNVDIPQAETLELP----EDMMIDDIEGNTNEEEINNNDLPSNMDIDESEV-QAKDTMDNTQDGFNEAAYDAMETDEIDDDSISNETKDGNKMGVEKIENNDEISDENQQESSKGEVPKERPNH-KVQLSTDDNQLEDMNIENEDQKTSSNQEQPNSDAPAENLFGVDGESGKANMASSKSSGNGSEQSLQETENQDSNNNSQRYKSKGEXXXXXXXXXXXXXXXXXXXXXXXXXXDANPHRS-LGDALK-----------------QWKRRLDNIVKGGENTSEQENNLEQNQIVNED---------QTFEFLENDDDSHDLQTMGAAAED--QIGAIDNNEQHYNYETNYAVEEN----------EIDYDNSQ------KDEITDALPTNDY--NH----------NSENEKGAILSRKIVKQDDDQQLFDEKFNPQSIYSSHFTHEPLTREEI-------ESLRQE----LEIKLTDWRQSGRDITKARELWQKYENLTYDLANGLCEQLRLILEPTLATKLKGDYRTGKRLNMKKIIPYIASDFKKDKIWLRRTKPSKRQYQVMIAVDDSKSMCETHSIQLAYETLALISKALSQLEVGDLSIVSFGEKVQLLHSFDRPF--SSEAGAQVLQQFTFEQNKTYVRSLMETSITLLEHAKNNGTKNKELWQLQLIISDGVCEDHESLKALVRKAAEARIMLVFIIVDNKPGKD-SIMSMNHVKYKSINGRMTIHMERYLDTFPFDYYVVLRDINALSETLADALRQYFSI 5487          

BLAST of Gcaud8403.t1 vs. uniprot
Match: A0A397IJ94_9GLOM (Midasin n=1 Tax=Diversispora epigaea TaxID=1348612 RepID=A0A397IJ94_9GLOM)

HSP 1 Score: 299 bits (765), Expect = 3.790e-77
Identity = 436/1895 (23.01%), Postives = 781/1895 (41.21%), Query Frame = 0

Query:  885 KSSTPLSIVVIGLENVLRKADEWNRLFATGETKLRAELVEVSRLVSRWRGVEMRSWPLLLAARKREFETQADKWVFLIYDAVIN--ETLSAGMHDNEQVN--------SALSTIDRFLRSSPSGEFCRRLAIVQSISHHL-----LSLNDARRDLAQFIGNSLRGICRFYHLFKDHLEQKLSQRYETVLQELKEFTNIVSYNPQEELGSAVEMSKGRDKNLDYFRLKALSDKTRRRVHKLCLKVDGILRVPFYEHMMDFTAGFGFDHPIILNGASKHDPVRPLGQDILTKTSRGIACDERDVLDSKHRSSILLPAALKMLSRFDTLRTRLV---TYGREIQSSSRTECEA-----ASAFSSKLRT--------MIRARVSKLREMRLGSDIQPKRRALIDLLKGLSRAGLSPFER-----------------PHAN---SMLRWLSTSDPSSCEVSNKIANHLYFSGIHQYQTLRNVSDSSTRNNDISAEEAGRIRAFCRDLLDKSAMER---TELNKCSAIVHC--LSKVA---DALTKGHQDILSTSRENPAFFGCFRDILFRLRKAKSTLQQISKLMSEAQRNMSGDADFVQGQGTLDEVLCFYRKVGKKSLSELQIIL----NTCCDKIGNQPKAFSLMDIDHVEYQELAYFDGSYKEWCETIRAYLSSLKETLHVGLSRAREVSEHNLVTEILSPLLSLVEDGISEESPLP-----SSPAQVASALYNSLLSNAEELVHSILISSQK----AMAWNGVRELLGENKSAAPPLGKDETELFPEGVIRRGHDGTVNLGSYLLLTRISSHFDANSRFLQSLIPLRDVLAWKETKELIG-IQNALGSILKEYLRSMLMPCLEKAIVHHHENLALLR-TLTNLFIGICKDGFCRPDLEEQTEGMMETEVKSGTGFGDVGNGDMSGAQDVSEAIEDEEQLLGLRDERD-KDLRDENENVRNEDGFDMKTDFEGTLEDVDSEGVQDMEGD----GEAEKQMGQEHGKGENLLNEKLWDSENETNKEQASELDSKQDEEESESQGNGKQGEWAVGDRRDRSHSKEMNEQNREEEKSELDASRVXXXXXXNAEEELGSYEEPEDLEKDASRDREHGDHRENGEQENCDTHSEDIQESGTEKQKKHGEEDHGMTDGYNSDFARGQEPDPCLPESDQPEVGEAESNCGSPMVGVGDEAEVLEYEGMDEDQTGDFHEDCQLSGLEEEADMGNEKTNPEDKLEHVKKDVSPDLAKTNEEEEAKSISSAQDD--IKSDGGEHCQ---ETEGDSDVLNGADADAKQPVSEKEHRSMNILPGRSTTGQADKEIEGSTEPFAHQGQGTLGFGSNMNDADGERNDIADRSSHDHQPGPGNQINKQNGEEEGRKLESSMKQDEQLEKGSSSMNTNPLR-LSDPEHLIKEWNECLRALRDDDEQLPKQTEG-----------ENDADAWQYETIDDPKENPQHSLGAATQKQQRPLPDERVAELDSESIRHKEAEPKPKLREAMSTQDRQKSHFRQEQSSSPQNPNESLGDLVHGDVEKPHPVRSAIDRERERNRIASAHIGQSESSIFETRGKPGCDEDERNEEPTPVTSQVLGWRMENLDHAQAKELWHALESKVASEAATLCEQMRLVLEPTKASRMGGGYRTGKRLNMRKVIEYVASDFRKDRIWLRRVKPDKRSYDVLIAIDDSASMLESEAGVMATESLALVISALSKLEVGRIAVASFGSTANLIRHFEEPLPINTESGAKLLQHFTFSQTETNIVNLLS-----FIHSQMGSTNNESTVEHISLALVISDGRLSHREEVRRQLRILKESNVLVAFFILDRGTVEENSIYNVQRVEYEA-NGKMSVR--PYMHDFPIDFYAVVQNMSSLPGTLADALRQWIEI 2675
            K ++P++ ++ GLE +L+K+++W   +A+ +  L++   E++ L+ +WR +E+  WP LL A+ + +E  A KW F +Y+ +I      S    +N+ +           +  +D+F++SS  GEF  RL ++ +   HL      +  + +  L     ++L  + ++Y  F + L   L  +++ + +EL +F  I S+                 K+ + + LK  + KT R + K   K   IL  P    + ++      DH  I +     D    +  ++L    + +      +  S+    ++    L M  RF  +   L    +Y  +   ++   C +     AS    +++T        M     S ++ M+L      K+++L+DLLK L R GL  F                   P      ++ +    ++     +  K A+  YF  I Q   LR+ S + ++  D+S++E  +   F  DLL     ER   TE  K     HC  LS V    D +   ++        N        +   RL+  K     I  ++  +         F Q Q T + V      +   S +  +II          +     K+F   D+ ++        + S K    T+  +L +L     +  S           T I +P+   +   I   + L      ++    A+ +   LL    +L+  +L+  Q      +  NG+ EL   ++SA+P    D         IR  H+  +N+G  L +  +   F+     +  L+   +    KE ++ I  +   +   +++Y   +++       V HH++L  L   L N F  I   GFC P+++E            GTG G+       G++DVS  IEDEEQ+LG ++E + K    +N+    E+G +M+ DFEG LEDV+    +D         + E+Q+GQ      ++++EK+W              D   D+E++E   N             +  S + +   +++    LD                                    +H E  +  N D    +  E     +    EE++ M D         ++ D                         G            +D    F+E  +   L E++D  N   + + K E +  D   +L +  + +E  +  S   D  +K    E  +   E + D       D    +P SE      N+       G++ KE   S    +++ Q                 +  D+  HD           QNG +  + + ++++Q E         + NP R L D    +++W   L  + + D   P+ +             +ND   ++Y T +D  E+    +  A  +Q R   D    +  +    H   E    + +    +     +F   +              +    E    +  +ID     N+     I +S+ S+ ET  +   +   ++     +  ++  WR    D  +A+ELW   E+     A  LCEQ+RL+LEPT A+R+ G YRTGKRLNM+K+I Y+ASDFRKD+IWLRR +P KR Y V+IA+DDS SM ++ +  +A E+LAL+  ALS+LEVG I++ SFG    ++  F++P   ++E+GA++LQ FTF Q +T + NL+        H++   ++     E   L L+ISDG     E ++  +R   E+ ++V F ++D  + E++SI ++ +V+Y++ +G+M++    Y+  FP D+Y +++++++L  TLADALRQ+  I
Sbjct: 3862 KLNSPIAKLLTGLEILLQKSEDWES-YASRDVSLKSNRDEITDLIIKWRKLELECWPKLLKAQDKYYEQFAHKWWFHLYECIIRPIHKFSHQSSNNKDITPLYKNHITEIVKILDQFIQSSNFGEFESRLELIHTFYLHLSAKYHFNKMNTQGSLYNQAADALWNVYKYYSQFLNELRSTLVNQHKPIEKELYQFVKIASW-----------------KDTNIYALKQSAQKTHRHLSKCIRKYKDILNKPISFIITEYQ-----DHLTIKSKEKVRDD--HVNHELLNLPEKWLI-HILPIDFSERTFELMSKQVLTMPDRFININKTLQKFKSYCLKDIFNNHAICNSHLDDFASEIILRIKTFQDETVPFMNEENKSFVKNMKL-----VKKKSLVDLLKELKRLGLHTFPNKKLVESQQNLGGYLLKLPRMQIELTLFKGQLLTEIGFFSIYEK-ADDYYFRIIAQINNLRHASANYSK--DLSSQEVCKALGFTEDLLSLLIQERQCFTEFEK-----HCEGLSGVVIQLDTIYSAYKSFSDNGSRNVVLLDSLYES--RLKIVKEMFDDILCMLIHSSAIFDIQKQFSQ-QNTENIVQNIQNWLNTISSARKEIISIFDRTILMSEYTIGKKSFLTTDVINI-------LENSVK----TLNEFLENLNHVCALLPS----------FTHIFAPIRDYINSRIGNLASLNINHNITNLDDSATVILGQLLHKVSDLLDDLLVIVQNLKKSCLQENGMAELSEIDQSASPSSMCDNN-------IRLKHEFILNMGKKLQVKSVIDRFNQIHSLVSKLMD-DEKFNNKECRDFITFLLQRIFPFIQQY--HIVVQYYSSLFVFHHKSLCKLELVLCNSFATIFTKGFCIPEMKENEXXXXXXXXLQGTGIGEG-----EGSKDVSNEIEDEEQILGTQNEAELKPSDSKNDKDDKENGINMENDFEGALEDVEKNNDEDXXXXXXXXSDLEEQIGQFDDNESDVVDEKMWG-------------DDSSDKEDTEKSSN------------KQXSSSKSDIVAKDDPNENLDDKAPKPQTPKQGXXXXXXXXXXXXXXXXXXXXXXXXEHNEQQDHFNVDIPQAETLELPDNMEIDDMEENYDMDD---------RDDDLXXXXXXXXXXXXXXXXXXXXXXATG------------QDDLNSFNELMETDELNEDSDSINTFPDNKQKEEQISIDNVDELIEQQDSDEDINPESKTQDHSLKKSQNERLKMPIEEKVDEQTSFNEDQAKSEPTSE------NVF---GIVGESGKETSASEVISSYEIQ----------------QNSYDQKEHDTINNXXXXXXXQNGNDN-KDIXNNVQQREHS--NDEIKDANPRRSLGDA---LEKWRRRLEIVNEHDSTNPEISRAKEMQETNIQDYDNDKQTFEY-TKNDDMEHDLQIMEGANDEQSRNFCDIDNGDDHNYEFNHMPEEIDENIIDRQEDEPMYIENFHDSEXXXXXXXXXXXXGSLFTRSEMKLDLEDSID-----NKFDLQVISKSQQSL-ETMSQLNVESLRQD-----LEIKLQEWRKSGRDIDKARELWQKYENLTYDLANGLCEQLRLILEPTLATRLKGDYRTGKRLNMKKIIPYIASDFRKDKIWLRRTRPSKRQYQVMIAVDDSKSMCQTHSIQLAYETLALISKALSQLEVGDISIVSFGEKVQILHPFDQPF--SSEAGAQVLQQFTFEQNKTYVRNLMETSIVLLEHARNNYSSRSQQKELWQLQLIISDGVCEDHESLKVLVRRAVEAQIMVVFIVVDNKS-EKDSIISMNQVKYKSVDGRMTLEMVQYLDSFPFDYYVILRDINALSETLADALRQYFSI 5586          

BLAST of Gcaud8403.t1 vs. uniprot
Match: A0A8H7SMB1_9FUNG (Midasin n=2 Tax=Mucoraceae TaxID=34489 RepID=A0A8H7SMB1_9FUNG)

HSP 1 Score: 281 bits (720), Expect = 6.810e-72
Identity = 476/1990 (23.92%), Postives = 793/1990 (39.85%), Query Frame = 0

Query:  840 PIGNDLEDELLDCIEEIQRLFFKENGNHPVLDGIMVAIDSVARVSKSSTPLSIVVIGLENVLRKADEWNRLFATGETKLRAELVEVSRLVSRWRGVEMRSWPLLLAARKREFETQADKWVFLIYDAVINETLSAGMHDNEQVNSA-----LSTIDRFLRSSPSGEFCRRLAIVQSISHHLLSLNDARRDLAQFIGNSL---------RGICRFYHLFKDHLEQKLSQRYETVLQELKEFTNIVSYNPQEELGSAVEMSKGRDKNLDYFRLKALSDKTRRRVHKLCLKVDGILRVPFYEHMMDFTAGFGFDHPIILNGASKHDPVRPLGQDILTKTSRGIACDERDVL----DSKHRSSILLPAALKMLSRFDTLRTRLVTYGRE-------------IQSSSRTECEAASAFSSKLRT-MIRARVSKLREMRLGSDIQPKRRALIDLLKGLSRAGLSP----FERPHANSML---------------RWLSTSDPSS--CEVSNKI-----ANHLYFSGIHQYQTLRNVSDSSTRNNDISAEEAGRIRAFCRDLLDKSAMERTELNKCSAIVHCLSKVADALTKGHQDILSTSRENP----AFFGCFRDILFRLRKAKSTLQQISKLMSEAQRNMSGDADFVQGQGTLDEVLCFYRKVGKKSLSELQIILNTCCDKIGNQPKA-----FSLMDIDHVEYQELAYFDGSYKEWCETIRAYLSSLKETLHVGLSRAREVSEHNL--VTEILSPLLSLVEDGISEESPLPSSPAQVASALYNSLLSNAEELVHSILISSQKAMAWNGVRELLGENKSAAPPLGKDE---TELFPEGVIRRGHDGTVNLGSYLLLTRISSHFDANSRFLQSLIPLRDVLAWKETKELIGIQNALGSILKE---YLRSMLM---PCLEKAIVHHHENLALLRTLTNLFIGICKDGFCRPDLEEQTEGMMETEVKSGTGFGDVGNGDMSGAQDVSEAIEDEEQLLGLR-DERDKDLRDENENVRNEDGFDMKTDFEGTLEDVDSEG------VQDMEGDGEAEKQMGQEHGKGENLLNEKLWDSENETNKEQASELDSKQDEEESESQGNGKQGEWAVGDRRDRSHSKEMNEQNREEEKSELDASRVXXXXXXNAEEELGSYEEPEDLEKDASRDREHGDHRENGEQENCDTHSEDIQESGTEKQKKHGEEDHGMTDGYNSDFARGQEPDPCLPESDQPEVGEAESNCGSPMVGVGDEAEVLEYEGMDEDQTGDFHEDCQLSGLEEEADMGNEKTNPEDKLEHVKKDVSPDLAKTNEEEEAKSISSAQDDIKSDGGEHCQETEGDSDVLNGADADAKQPVSEKEHRSMNILPGRSTTGQADKEIEGSTEPFAHQG----QGTLGFGSNMNDADGERNDIADRSSHDHQPGPGNQINKQNGEEEGRKLESSMK----QDEQLEKGSSSMNTNPLRLSDPEHLIKEWNECLRALRDDDEQLPKQTEGENDADAWQYETIDDPKENPQHSLGAATQKQQRPLPDERVAELDSESIRHK----------------------------------EAEPKPKLREAM--STQDRQKSHFRQEQSSSPQNPNESLGDLVHGDVEKPHPVRSAIDRER--------------ERNRIASAHIGQSESSIFETRGKPGCDEDERNEEPTPVTSQVLGWRMENLDHAQAKELWHALESKVASEAATLCEQMRLVLEPTKASRMGGGYRTGKRLNMRKVIEYVASDFRKDRIWLRRVKPDKRSYDVLIAIDDSASMLESEAGVMATESLALVISALSKLEVGRIAVASFGSTANLIRHFEEPLPINTESGAKLLQHFTFSQTETNIVNLLSFIHSQMG------STNNESTVEHISLALVISDGRLSHREEVRRQLRILKESNVLVAFFILDRGTVEENSIYNVQRVEYEA-NGKMSVR--PYMHDFPIDFYAVVQNMSSLPGTLADALRQWIEITN 2677
            PI N  +  +L+ +E+           H +L+ ++V  D +   S  S P++  + G+E +L+K+++W   +A     L+ +  E+  L+  WR +E+  WP LLAA+++  +  A  W F +YD V N +  +   D+E VN+      L  +D+F++ +   E   RL ++ S           +  L+ F G  L         R +  +Y  F++H    L+Q  + + ++LK+F  I ++                 K+++ + L+  + KT R++HK   K   +L       M+   A +  +H +   G  K    + + + ++ + S+        V+    D    SS   P   + LS       R+  Y R+             +++      E    F  +  + M     S ++  +L      K++AL+D LK L R GL       +  +A++ +               R L     SS  C  ++ +     AN  YF  I +   LR +S ++  + D+S  E  R  A    +      ER+ L++    +  L   A  L   +    S S+  P    A  GC      RL   K  + +++  +++A   +S  A  V GQ TL ++     +   +S+ ++Q  ++ C  +    P++     FSL+  D  E  +      S+ E  E I         TL V   +    S H L  +T+ +  +  L    + EE    +    +       L      L+ + L+S Q     N   E   + KS     G++E   TE   +  IR        L S L       H +A S+    ++ +   L  +   E   + N +  +L+E   +L+  ++     L K +VHH     +   L N F  I   GFC P   +  E      V SGTG            +DVSE IEDEEQ+LG + +ER+ D + + +  +N  G DM+ DF+G LEDV+ +                   Q+G       + +++K+W  E E N         K+ ++  E QGN  Q                       E++SE+ A                         KD           EN +Q +      D            GEE  G                         +V   E+  G  +     EAE LE   + +D   D            + DM N+  + ++K  +  ++  P+     E+EEA++   A DD+        Q  +G+ + +  A A       E +        G       D+E E + E     G    Q   G          E N   +R   D      NQ   Q   E G++ +SS      +D+  E                    +  N+      DD E+            A Q ++      NPQ SLG A +K +R     R+A+LD E+   +                                  EA     +  A     QD +     +E+  + +   E   D    DV+     R  +D                 E+  +  + I   + S+         D +   +E   + +QV  WR E  D  +A+ELW   E+     A  LCEQ+RL+LEPT A+++ G YRTGKRLNM+K+I Y+AS F+KD+IWLRR KP KR Y V+I++DDS SM ES +  +A E+L+L+  ALS+LEVG I++ SFG    L+  F++P    +ESGA ++Q FTF+Q +T + NL   I + +G       T+     E   L L+ISDG       +R  +R   +  +++ F ++D    E++SI N+  V+Y   +GK  ++  PY+  FP  ++ V++++++LP  L+DALRQ+    +
Sbjct: 3798 PIVNRFKARILELVEQWPE--------HAILEQLIVICDRILTFSILS-PVAKFLTGIELLLQKSEDWEA-YAAKHVSLKDQREELIALIVSWRQLELNCWPKLLAAQEQYHQDAAFTWWFHLYDTVNNTSFES---DSETVNTKNTKELLGALDQFIQCASIIELEPRLKMIDSFYRQ----TKIQAQLSNFEGEKLNYERTATILRNVYLYYSQFQEHANTMLAQLRKPIEKDLKDFVKIATW-----------------KDVNIYALRQSAAKTHRQLHKCIRKYREVLN----NSMLTIIANYNEEHAMYQFGDDKRY-AKDVNRGLVDQLSQPSIWTGTTVMPESNDDFDWSSA--PEVKQHLSNLQVTLNRMRAYCRKDVFVVDEETKELPLENFITEMIEQVKYFQKETPSIMTEENKSTVKNQKL-----LKKKALVDFLKELRRLGLKSRPGTMKEQNADTAILFRHQVADLESVIQSRDLQKEKLSSYTCATADMVEQWKKANDYYFRCIARMTHLRTISTTNV-SKDLSMLEVERSLAATEHMFSLITKERSVLSRVEGRMQILQGAAVQLASLYD---SFSQGKPIASDAELGC------RLTNHKVHIDKLALFLNQAVVVVSLQAGSV-GQRTLKDL-----EEMARSIQKIQKTVDYCFVQRYLYPRSATGLEFSLLSTDIEEMIK------SHSEKIELIH--------TLVVSAMQVLPQSSHVLFPITQYIESIGHLPITQVQEEEESEAEKVTIVD-----LREKIYGLIDAALVSIQDLKKANAAPE---KPKSILTEEGEEEEEETEDMSQDYIRLQSTKQAKLASAL-------HLEAASKRCVEVLTIAHTLMSRS--ESADVVNEISRLLQESYPFLQQYMLIVQHTLSKMLVHHKAMAKMTYCLVNSFSIIITKGFCMPAGADDGEEGDADGVMSGTGMXXXXXN-----KDVSEEIEDEEQVLGTQNEERNNDDKQDTKEEKN--GMDMENDFDGNLEDVEHDDEXXXXXXXXXXXXXXXXXQIGDVDDMDPDAVDDKMWGDEAEENL--------KESDKTVEDQGNQDQ-----------------------EQESEIVA-------------------------KD-----------ENDDQPDSKGEKPDKNXXXXXXXXADGEEGDGXXXXXXXXXXXXXXXXAXXXXXXXXDV---ENKAGEQLNAEIPEAETLE---LPDDMNMDGXXXXXXXXXXXQ-DM-NDPMDMDEKAPNQGEEQLPE-----EDEEAEAFHDALDDVD-------QGMDGEDEEMADASAQMDTEAGEGDDEEEKNASG-------DEENEENEELAPEVGDSEQQKKXGXXXXXXQDVDEENKAQNREQPDSDATADNQFGVQG--ESGKQSKSSAGKKEGEDDTAEXXXXXXXXXXXXXXXXXXSERGANQSNEEEADDAEKEXXXXXXXXXXXASQAQS------NPQRSLGDALEKWRR-----RLADLDDEAENEEXXXXXXXXXXXXXXXXXXXXXXXXXSFEYVKNDDEAHDMQTMGNAQPDQVQDLKMGGMDEEKEDNNETSGEMEVDDQVDDVDTMPLARDTLDMSGAGDTQGAILSKKLPEQQLMDESEILTMDESVVSREPLEQEDIERMRDE---LETQVSDWREEGRDINKARELWQGYENLTHDLAMGLCEQLRLILEPTLATKLKGDYRTGKRLNMKKIIPYIASQFKKDKIWLRRTKPSKRQYQVMISVDDSKSMSESHSVQLAYEALSLISKALSQLEVGSISITSFGERVRLLHPFDQPF--TSESGANVIQQFTFAQQKTYVKNL---IETSLGLFEGAKHTSGPGNAELWQLQLIISDGICEDHNTLRALVRSALDQQIMMIFIVVDNKP-EKDSILNMTNVKYTIKDGKYGIQMNPYLETFPFQYFMVLRDINALPEVLSDALRQYFSFVS 5571          

BLAST of Gcaud8403.t1 vs. uniprot
Match: A0A507ER43_9FUNG (Midasin n=1 Tax=Spizellomyces sp. 'palustris' TaxID=117820 RepID=A0A507ER43_9FUNG)

HSP 1 Score: 280 bits (717), Expect = 1.520e-71
Identity = 447/1909 (23.42%), Postives = 771/1909 (40.39%), Query Frame = 0

Query:  887 STPLSIVVIGLENVLRKADEWNRLFATGETKLRAELVEVSRLVSRWRGVEMRSWPLLLAARKREFETQADKWVFLIYDAVINETLSAGMHDNEQVNS-------ALSTIDRFLRSSPSGEFCRRLAIVQSISHHLLSLN-DARRDLAQFIGNSLRGICRFYHLFKDHLEQKLSQRYETVLQELKEFTNIVSYNPQEELGSAVEMSKGRDKNLDYFRLKALSDKTRRRVHKLCLKVDGILRVPFYEHMMDFTAGFGFDHPIILNGASKHDPVRPLGQDILTKTSRGIACDERDVLD-SKHRSSILLPAALKMLSRFDTLRTRLVTYGREIQSSSRTECEAASAFSSKLRTMIRARVSKLREMRLGSDIQP-----------KRRALIDLLKGLSRAGLSP---------------FERPHANSMLRWLSTSDPSSCEVSNKI-----ANHLYFSGIHQYQTLRNVSDSSTRNNDISAEEAGRIRAFCRDLLDKSAMERTELNKCSAIVHCLSKVADALTKGHQDILSTSRENPAFFGCFRDILFRLRKAKSTLQQISKLMSEAQRNMSGDADFVQGQGTLDEVLCFYRKVGKKSLSELQIILNTCCDKIGNQPKAFSLMDIDHVEYQELAYFDGSYKEWCETIRAYLSSLKETLHVGLSRAREVSEHNLVTEILSPLLSLVEDGISEESPLPSSPAQ-VASALYNSLLSNAEELVHSILISSQKAM------AWNGVRELLGENKSAAPPLGKD---ETELFPEGVIRRGHDGTVNLGSYLLLTRISSHFDANSRFLQSLIPLRDVLAWKETKELIGIQNALGSILKEYL---RSMLMPCLEKAIVHHHENLALLRTLTNLFIGICKDGFCRP----DLEEQTEGMMETEVKSGTGFGDVGNGDMSGAQDVSEAIEDEEQLLGLRDERDKDLRDENENVRNEDGFDMKTDFEGTLEDVDS------EGVQDMEGDGEAEKQMGQEHGKGENLLNEKLWDSENETNKEQASELDSKQDEEESESQGNGKQGEWAVGDRRDRSHSKEMNEQNREEEKSELDASRVXXXXXXNAEEELGSYEEPEDLEKDASRDREHGDHRENGEQENCDTHSEDIQESGTEKQKKHGEEDHGMTDGYNSDFARGQEPDPCLPESDQPEVGEAESNCGSPMVGVGDEAEVLEYEGMD--EDQTGDFHEDCQLSGLEEEADMGNEKTNPEDKLEHVKKDVSPDLAKTNEEEEAKSISS------------------AQDDIKSDGGEHCQETEGDSDVLNGADADAKQPVSEKEHRSMNILPGRSTTGQADKEIEGSTEPFAHQGQGTLGFGSNMNDADGERNDIADRSSHDHQPGPGNQINKQNGEEEGRKLESSMKQDEQLEKGSSSMNTNPLR-LSDPEHLIKEWNECLRALRDDDEQLPKQTEGENDAD------AWQYETI-DDPKENPQHSLGAATQKQQRPLPDERVAELDSESI----------RHKEAEPKPKLREAMSTQDRQKSHFRQEQSSSP----QNPNESLGDLVHGDVEKPHPVRSAIDRERERNRIASAHIGQ-SESSIF---ETRGKPGCDEDERNEEPTPVTSQVLGWRMENLDHAQAKELWHALESKVASEAATLCEQMRLVLEPTKASRMGGGYRTGKRLNMRKVIEYVASDFRKDRIWLRRVKPDKRSYDVLIAIDDSASMLESEAGVMATESLALVISALSKLEVGRIAVASFGSTANLIRHFEEPLPINTESGAKLLQHFTFSQTETNIVNLLSFIHSQMGSTNNEST----VEHISLALVISDGRLSHREEVRRQLRILKESNVLVAFFILDRGTVEENSIYNVQRVEY---EANGK--MSVRPYMHDFPIDFYAVVQNMSSLPGTLADALRQWIEITN 2677
            ++P+  ++ G+E +L+K  +W   +A+ E  L++ +  +  L+ RWR +E++SW  +L    R  E +  K  F ++  VI   +   + + E  N+        L+ +D+FL SS  GE+  R+ ++ +   HL +++ +   +    + N L  + R++ LF D +   +    + + +EL E+  I ++                 K+++ + LK  +++T   +HK   K   IL +P  + M        +   + + G      V+ +   +    +  +      V   S     +L     +M+++ D L  R+  + R++ SS  T  E  +     L   I  R+ + +EM   + ++P           +++AL+DLLK LS  GLS                F  P  N     L      +  V++ I     A+  ++  + +   LR +  S++   D++  E  +  +F   LL  +  +R  L++ +     LS +A  + + +    S S EN A    ++ I+  LR  K  + +   ++   Q  +  DA +V+ +    EV        +++L  +++ L++    + ++    +   +  V  +               I  ++  L  ++H  +S        N + ++L   + +V   ++E      + A  + +A      S  ++ V ++L+S Q  M      A NG    + +++  A  L K    +      G+ R GH G V      LL+ +  H D  S      I  R   A         +   +  +L +YL   R  L  C     + H   L L   L N F  + KDGFC P    D     +  ME  V +GTG G+       G +DVS+ IE+EEQ+ G+++E       + +    E+G +M TDF+G LEDVD+                    QMG   G   N+++EKLW              D  +DE                             + N ++EK+E DA           E E+ + E       D  +D E                         ++                  F      DP   +               P   +G EAE       D   DQ+GD  +   LS   E+ + GNE++  E+K          D   TN EEE                        A+ +                       A  K+   E +      L     T   D +I+ ST+P+  +G       SN+     + ++++D    D + G  N+      E+  R+ + S  +     +   +M+ NP R L D    +K+W   L+ + +  E+     E  ND        A ++E + +D +     ++  AT  Q   +  + +A+   E             H+E  P  +   +    D QK    +  S +     +   + + + V GD E    V +    E E + I   H    SE       E     G D  + +E    +   V  WR    D  +A+ELW    +     +  LCEQ+RL+LEPT A+++ G YRTGKRLNMRK+I Y+AS F+KD+IWLRR KP KR+Y ++IAIDDS SM ES +  +A ESLA++  AL++LE G ++V SFG    L+  F+ P   + ++GA +++ FTF Q  T++  ++      +    ++S      +   L LVISDG     E V+  +R   E  ++V F +LD    E++SI  +  V Y    + G+  + +  YM  FP D+Y VV+++ +LP  LAD LRQ+    N
Sbjct: 3739 TSPIMKLLTGIELLLQKCQDWEP-YASREVSLKSHMENIIALIVRWRKLELKSWRQILEVEDRRSEEECSKLWFHLWKTVIGLAVQDEVQEIEDANTNRELVKDLLTMLDQFLLSSSLGEYGTRVDMINTFRQHLCAISLEVDTNRLDTVINVLFNVHRYFALFLDRVSSTIDNLKKPIRKELNEYVQIATW-----------------KDVNVYALKESANRTHHHLHKFVRKYRTILDMPAKDAMS------AYHDTVAIEGGKASRTVKAVVNRLAKYGAEAVKSAALSVPSLSALEQQVLRAPCERMVAKVDHLVQRMQKHTRKLFSSEETNSECVAV--DDLAITIIERIKEFQEM--NATLEPGSKAAKGQKTMRKKALVDLLKYLSYLGLSSRCTIKYSEQQDSAFLFSGPKVNLKDALLGILYKENTLVADTIGLWEKADSYFYRILARMAKLRELQISASV--DLTRVEVEKSMSFLDHLLHFAIQQRAGLSQVADHYEPLSAMATQIAEVYGTGESES-ENVALVPGWK-IVKVLRGQKDAIDEAMAVV--IQGVILADA-YVRSKQEGIEV--------RRALETVRVQLSSLKKGVDDRYALHAASTVSAVAVESPVRMMFKNAVALSEIERHVKDLT-SIHPDVSYIL-----NDLADVLQKKVEIVNSTLTEMFGENEATADGLDNAAVEEFTSRVDDAVTAMLLSFQALMSIDETAAPNGATNGVCDDEDDAFGLRKRCLIDAHQRTVGLFRSGHLGKVLEAFNNLLSGV--HMDTQS------IQSRRYFA--------SLVRRVYPLLDQYLLAVRRSLFDCT----LQHKSMLKLAYILCNTFGQLYKDGFCVPRDAKDDGADDDSTMEDNV-AGTGIGEG-----EGNKDVSDEIENEEQVEGMQNEAPAKPDPQKKIDDEENGIEMDTDFDGKLEDVDAGDXXXXXXXXXXXXXXXXXXQMGDVDGNA-NVVDEKLWG-------------DDDKDE-----------------------------DVNGQDEKTERDAP----VQDAGGETEMAAKE-------DGGKDEEESSXXXXXXXXXXXXXXXXXXXXXADEDGXXXXXXXXXXXXXEEKFEDSHGIDPKAADEKXXXXXXXXXXMELPD-DMGIEAEEXXXXXRDGGRDQSGDRMD---LSS-NEDMNHGNEESIEEEK----------DEESTNVEEEKXXXXXXXXXXXXXXXXXLXXXXLAEQEXXXXXXXXXXXXXXXXXXXXWXSAHDKEETEEADSE----LSPEDVTQAVDDQIQSSTQPYGVKGASGEKSASNV-----QPDEVSDEIPEDGENGIANEDFAPGAEKSQRRSQHSRDEGRDKSRKDENMDPNPRRSLGDA---LKKWMSRLKNISESTEEEKTDREEFNDEPLLDGDKALEFEFVKEDDEAADAQAMADATADQFEEMDRKALADDKEEKEYGTDDAMDVDEHQEDAPDEQGTTSREPDDTQKKGRSENPSGAIDLGLEKDGKEVENKVRGDEEDVGDVDTMDVDEEEVDDIEWNHPRTLSEGDTLADDEADESTGMDSHQYDELRLELEQSVAAWRESGQDPREAEELWRNYTALTRDLSFALCEQLRLILEPTLATKLKGDYRTGKRLNMRKIIPYIASQFKKDKIWLRRTKPSKRTYQIMIAIDDSRSMAESRSVQLAYESLAVITKALTQLEAGDVSVLSFGEDVKLLHPFDRPF--SDDAGADVIRRFTFKQDRTHVRQMMETAMGILDHARSQSAGGPGSDLWQLQLVISDGICEDHELVQALVRKAAEQRIMVVFIVLDNRR-EKDSITKMTNVTYGVDHSTGRPTLQMSRYMDTFPFDYYVVVKDIGTLPEVLADTLRQYFMFVN 5488          

BLAST of Gcaud8403.t1 vs. uniprot
Match: UPI001455D253 (midasin-like n=1 Tax=Asterias rubens TaxID=7604 RepID=UPI001455D253)

HSP 1 Score: 280 bits (716), Expect = 1.990e-71
Identity = 490/1986 (24.67%), Postives = 796/1986 (40.08%), Query Frame = 0

Query:  862 KENGNHPVLDGIMVAIDSVARVSKSSTPLSIVVIGLENVLRKADEWNRLFATGETKLRAELVEVSRLVSRWRGVEMRSWPLLLAARKREFETQADKWVFLIYDAVINETLSAGMHDN-----------EQVNSALSTIDRFLRSSPSGEFCRRLAIVQSISHHLLSLNDARRDLAQFIGNSLRGICRFYHLFKDHLEQKLSQRYETVLQELKEFTNIVSYNPQEELGSAVEMSKGRDKNLDYFRLKALSDKTRRRVHKLCLKVDGILRVPFYEHMMDFTAGFGFDHPIILNGASKHDPVRPLGQDILTKTSRGIACD---ERDVL---DSKH-RSSILLPAALKMLSRF-------------DTLRTRLVTYGREIQSSSRTECEAASAFSSKLRTMIRARVSKLREMRLGSDIQPKRRALIDLLKGLSRAGLSPFERPHANSMLRWLSTSDPSSCEVSNKIANHLYFSGIHQYQTLRNVSDSSTRNNDISAEEAGRIRAFCRDLLDKSAMERTELN----------------KCSAIVHCLSKVADALTK-----------------------------GHQDILSTSRENPAFFGCFRDILFRLRKAKSTLQQISKLMSEAQRNMSGDADFV------QGQGTLDEVLCFYRKVG------KKSLSELQIILNTCCDKIGNQPKAFSLMDIDHVEYQELAYFDGSYKEWCETIRAYLSSLKETLHVGLSRAREVSEHNLVTEILSP----LLSLVEDG--------ISEESPLPSSPAQVASALYNSLLSNAEELVHSILISSQKAM-AWNGVRELLGENKSAAPPLGKDETELFPEGVIRRGHDGTVNLGSYL---LLTRISSHFD-ANSRFLQSLIPLRD----VLAWKETKELIGIQNALGSILKEYLRSMLMPCLEKAIVHHHENLALLRTLTNLFIGICKDGFCRPDLEEQTEGMMETEVKSGTGFGDV---GNGDMSGAQDVSEAIEDEEQLLGLRDERDK----------DLRDENENVRNEDGFDMKTDFEGTLEDVD----SEGVQDMEGDGEAEKQMGQEHGKGENLLNEKLWDSENETNKEQASELDSKQDEEESESQGNGKQGEW-AVGDRRDRSHSKEMNEQNREEEKSELDASRVXXXXXXNAEEELGSYEEPEDLEKDASRDREHGDHRENGEQENCDTHSEDIQESGTEKQKKHGEEDHGMTDGYNSDFARGQEPDPCLPESDQPEVGEAESNCGSPMVGVGDEAEVLEYEGMDEDQTGDFHEDCQLSGLEEEADMGNEKTNPEDKLEHVKKDVSPDLAKTNEEEEAKSISSAQDDIKSDGGEHCQETEGDSDVLNGADADAKQPVSEKEHRSMNILPGRSTTGQADKEIEGSTEPFAHQGQGTLGFGSNMNDADGERNDIADRSSHDHQPG--PGNQINKQNGEEEGRKLESSMKQDEQLEKGSSSMNTNPLRLSDPEHLIKEWNECLRALRDDDEQLPKQTE-GENDADAWQ------YETIDDPKEN-PQHSLGAATQKQ---QRPLP------------DERVAELDSESIRHKEAEPKPKLREAMSTQDRQKSHFRQEQSSSPQNPNESLGD-----LVHGDVE-----KPHPVRSAIDR-ERERNRIASAHIGQSESSIFETRGKPGCDEDERNEEPTPVTSQVLGWRMENLDHAQAKELWHALESKVASEAATLCEQMRLVLEPTKASRMGGGYRTGKRLNMRKVIEYVASDFRKDRIWLRRVKPDKRSYDVLIAIDDSASMLESEAGVMATESLALVISALSKLEVGRIAVASFGSTANLIRHFEEPLPINTESGAKLLQHFTFSQTETNIVNLLSFIHSQMGSTNNE-----STVEHISLALVISDGR---LSHREEVRRQLRILKESNVLVAFFILDRGTVEENSIYNVQRVEYEANGKM-SVRPYMHDFPIDFYAVVQNMSSLPGTLADALRQWIEI 2675
            +E   HP L  +++ ID +     +S PL   V GLE +L KA EW    A+    +R  L +++ LV +WR +E+R W   L       E  A KW F IY  V +   S    D            E V   ++ + +F+  S  GEF  RL ++ S  H   ++  +     + + + L  +  +Y  F   +E ++  R   + +ELK +  I  +N                 +++++ +K   +K+ + +HK   K +  LR P       FT     +   I  G S     R   + +  +  +    D      VL    S H R S LLP   K+   F             D L   +V+  +E+Q    T+     A   K ++ +R R+            Q KR+AL DL + LS  GLS          L  + TS+ +   +   I              L    D+  +  D++  E  R+   C+    KS   R  L                 +C      L+++A +  +                             G+  ++   +   ++    + ++  +R+  +TLQ    L +  +RN  G+A  V      +   TLD +    RK        +  L E Q +L T   K+ ++    +  D+  + + ++   D S +E  + I   LS +++   +     + V + +L T ++S     L  L+E G          EE          +S L     S +E L+ SIL+  Q  + A    R+ + E    A    +D+ E F    ++ GH    +L S L   ++T    H        LQ L   RD    V   +E    + +   L  +L +YL  ++   L +++  H     LL  L  +F  +   GFC P      E   E   +  T F D+   G G+  G +DVS+ IE+E+Q+   + + +K          DL+DE      E G +M  DF+G L D +     E  +D + + E EKQMG     G ++L+E++W  E +              EE  E +G   + +  A  D +  +  K            E   +  XXXXXX      G Y + E   + A+   E                                  D G  D  N D     E +P   E+  P+  E +   G       DE E       D+D+             +E  D+G E                   A   E+E                G   Q+ +GD  +    +AD K  ++   H    +       G++ ++ E   E    +  G      N    + E    A+ S  +   G  P   I ++N        +S  +Q+ + + G S  + +     +  H      + LR     D+Q  KQ E G ND    +      YE I D         + AA++ Q   ++P+P            DE V  LD +    KE +   K      ++ +      +E+ S   NP+ + GD        GDV      +   + + ID    +    +  ++ + E+   E             E+   + SQ  G   E     +A+E WH  ES  +  A  LCEQ+RLVLEPT+AS++ G +RTGKR+NMRKVI Y+AS FRKD+IWLRR KP KR Y +L+A+DDS+SM ++ +  +A ESLA++ +AL+ LE G +AV SFG +  L+  F E      +SGAK+L+ FTFSQT+T I  LL++  + M S  +      S++E   L LV+SDGR   L   E+V+  +R  +++NV + F  LD     ++SI +++   +   G++  +  YM  FP  FY ++++++S+P TL+DALRQW E+
Sbjct: 3650 EEWPEHPTLKQLVMLIDRILEFPITS-PLMKFVTGLEILLEKAQEWESN-ASRAVSVRDHLEKLTDLVIQWRKLELRCWSACLDTVAYRQEQSATKWWFHIYQLVQSHLRSEATTDQDDRQDGTEGRVEGVEGVIAALQQFMEGSSLGEFPTRLQMLYSF-HCQTAMQPSSPKQLELL-SILWNLHCYYKQFLSAVESEIKSRRAPIEKELKGYVKIARWN-----------------DINFWSVKLAVEKSHKTLHKFSKKFETELREP---SKCVFTDSQIAEKEEISAGESLGTSSRTYNEFLTPEVYKSYIGDIPPSATVLKGDSSLHARLSSLLPRMTKLCRSFFKKCPYPALTTAVDELTGEIVSGVKELQGLDVTK----EADKEKQKSQVR-RIQ-----------QRKRKALADLFRYLSTIGLS------YRKGLAVVVTSNQNEALMVPPI-------------NLAVALDTKEKGMDLNQSEMVRLWEGCQSHFVKSIARRAALQMALAVPSKELGVGNIERCKGFTEHLTQLAASQRRQITDITQGIVDTRSFLYQLQQLKPTTDCHGNNPLIPPQQSTASWVTTLKGLVDYIRE--TTLQYGMLLDACPERN--GEATDVLSPLPREHLATLDTI----RKGDSQWVETRNKLQECQTVLETISQKVLSRANLITNQDLQLIFWSDVELLDESVEE-LQLISEILSDVEQLFILP----QGVGKSSLATLLISARERLLKMLLEYGHWKRWTVCCVEEDAGQDEMQTGSSKLLAEFESRSESLLASILLVVQSLVKAQTKERDNIDEGHQEADDKEEDQEEFFD---LQEGHL-VKHLSSALSTDVVTLDLGHMSKVTGSLLQDLKETRDRCQSVREIREFNSCVMLVLRLVPVLHQYL-GLVSFTLNQSVACHRTTCKLLSVLLGIFTELAGKGFCLP-----AEFSDELGGEGATDFQDIEEGGIGEGEGMKDVSDQIENEDQIQDTKKQGEKEEEEDFSNQPDLKDE------EHGIEMSDDFQGKLHDGEPQDGEESDEDKDAEHEMEKQMGDVDQPGADMLDERMWGDEEDXXXXXXXXXXXXXXEEFGEGKGEESRTDMVAKDDNQGAADDKGDXXXXXXXXXXEPGENNXXXXXXXXXXXXXGEYNDDEVDPRKANEPPE-------------------AXXXXXXXXXXXXNLDGGKMDEDNQDXXXXXETNPFDIETKLPDEKEHQDEIGEQN---DDEGEAXXXXXGDQDEA---------RKEKEXXDVGGEXX--------XXXXXXXXXAGGMEQEXXXXXXXXXXXXXXXVGLEPQDEDGDEPIPEDTNADYKPDLA---HHPQAMPEPAENYGKSQEKTETMQEQSIQEDAGKSEEEKNK---EKENVGSAESSLQEGHEGAVPSKTIPREN--------KSDQRQNYKRQPGQSDADRSLGSADEKVH------KRLRTTETPDDQQEKQAEQGPNDEQVEKDETSDLYEHIKDATSRYDTQMMDAASEDQLMEEQPIPNQEDEDAVAMETDEEVVPLDEDG---KEKDESGKNESVPGSKIKAGERRAKEEESDEPNPDVTQGDEAVDGTASGDVSDISKMQGSTIHTTIDLLSHDATPQSFMNMEELEALRLEM------------EQQLSMLSQKQGTAEEE---REAQETWHRYESLTSGLARDLCEQLRLVLEPTRASKLRGDFRTGKRINMRKVIPYIASGFRKDKIWLRRTKPSKRQYQILLAVDDSSSMADNRSRQLAFESLAVISNALTWLEAGELAVCSFGESVQLLHPFHEQF--TDQSGAKILRQFTFSQTKTKIGQLLNYATASMLSARSRLQTGGSSMETSQLLLVVSDGRGLFLEGMEKVKAAVRAARDANVFLVFLALDNPE-NKDSILDIKVPIFGGAGELPQISTYMEHFPFPFYIILKDINSMPETLSDALRQWFEL 5467          

BLAST of Gcaud8403.t1 vs. uniprot
Match: A0A433PX55_9FUNG (Midasin n=1 Tax=Endogone sp. FLAS-F59071 TaxID=2340872 RepID=A0A433PX55_9FUNG)

HSP 1 Score: 275 bits (704), Expect = 5.010e-70
Identity = 468/1980 (23.64%), Postives = 782/1980 (39.49%), Query Frame = 0

Query:  840 PIGNDLEDELLDCIEEIQRLFFKENGNHPVLDGIMVAIDSVARVSKSSTPLSIVVIGLENVLRKADEWNRLFATGETKLRAELVEVSRLVSRWRGVEMRSWPLLLAARKREFETQADKWVFLIYDAVINETLSAGMHD---NEQVNSALSTIDRFLRSSPSGEFCRRLAIVQSISHHLLS-----------LNDARRDLAQFIGNSLRGICRFYHLFKDHLEQKLSQRYETVLQELKEFTNIVSYNPQEELGSAVEMSKGRDKNLDYFRLKALSDKTRRRVHKLCLKVDGILRVPFYEHMMDFTAGFGFDHPIILNGASKH--DPVRPLGQDILTKTSRGIACDERDVLDSK---HRSSILLPAALKMLSRFDTLRTRLVTYGRE--IQSSSRTECEAASAFSSKLRTMIRA-------RVSKLREMRLGSDIQPKRRALIDLLKGLSRAGLSP---------------FERPHAN--SMLRWLSTSDPS-SCE--VSNKI------ANHLYFSGIHQYQTLRNVSDSSTRNNDISAEEAGRIRAFCRDLLDKSAMERTELNKCSAIVHCLSKVADALTKGHQDILSTSRENPAFFGCFRDILFRLRKAKS-----TLQQISKLMSEAQRNMSGDADFVQGQGTLDEVLCFYRKVGKKSLSELQIILNTCCDKIGNQPKAFSLMDIDHVEYQELAYFDGSYKEWCETIRAYLSSLKETLH------VGLSRAREVSEHNLVTEILSPLLSLVEDGISEESPLPSSPAQVASALYNS-----LLSNAEELVHSILISSQKAMAWNGVRELLGENKSAAPPLGKDETEL-FPEGVIRRGHDGTVNLGSYLLLTRISSHFDANSRFLQSLIPLRDVLAWKETKELIGIQNALGSILKEYLRSMLMPCLEKAIVHHHENLALLRTLTNLFIGICKDGFCRPDLEEQTEGMMETEVKSGTGFGDVGNGDMSGAQDVSEAIEDEEQLLGLRDERDKDLRDENENVRNED-GFDMKTDFEGTLEDVDSEGVQDMEGDGEAEKQMGQEHGKGE------NLLNEKLWDSENETN--------------------------------------KEQASELDSKQDEEESESQGNGKQGEWAVGDRRDRSHSKEMNEQNREEEKSELDASRVXXXXXXNAEEELGSYEEPEDLEKDASRDREHGDHRENGEQENCDTHSEDIQESGTEKQKKHGEEDHGMTDGYNSDFARGQEPDPCLPESDQPEVGEAESNCGSPMVGVGDEAEV----LEYEGMDEDQTGDFHEDCQLSGLEEEADMGNEKTNPEDKLEHVKKDVSPDLAKTNEEEEAKSISSAQDDIKSDGGEHCQETEGDSDVLNGADADAKQPVSEKEHRSMNILPGRSTTG--QADKEIEGSTEPFAHQGQGTLGFGSNMNDADGERNDIADRSSHDHQPGPGNQINKQNGEEEGRKLESSMKQDEQLEKGSSSMNTNPLR-LSDPEHLIKEWNECLRALRD--DDEQLPKQTEGENDADA-------WQYETIDDPKENPQHSLGAATQKQQRPLPD----ERVAELDSESIRHKEAEPKPKLREAMSTQDRQKSHFRQEQSSSPQNPNESLGDLVHGDVEKPHPVRSAIDRERERNRIASAHIGQSESSIFETRGKPGCDEDERNEEPTPVTSQVLGWRMENLDHAQAKELWHALESKVASEAATLCEQMRLVLEPTKASRMGGGYRTGKRLNMRKVIEYVASDFRKDRIWLRRVKPDKRSYDVLIAIDDSASMLESEAGVMATESLALVISALSKLEVGRIAVASFGSTANLIRHFEEPLPINTESGAKLLQHFTFSQTETNIVNLLS-----FIHSQMGSTNNESTVEHISLALVISDGRLSHREEVRRQLRILKESNVLVAFFILDRGTVEENSIYNVQRVEYE-ANGKMSVRP--YMHDFPIDFYAVVQNMSSLPGTLADALRQWIEI 2675
            PI N L+  +L+ +++           H  L  ++   D +A  S +S P++ ++ G+E +L+K+++W   +A+ +  +++    +  L+  WR +E+  WP LLAA+    +  A KW F +Y+ +I   L +  ++   N +    L T+D+FL+SSP  EF  RL ++ +   HL +           LN+  R + + + + L  + ++Y  F      KL Q  + + ++LKEF  I S+                 K+++   LK  + +T R++HK   K   +L       M D  A +  + PI      KH  D    + Q + +  S  I     DV   +   HR    +    +  +  +   T+L  Y  E  +   S T       F + + T I+         +++  +  + +    K++AL+D LK L R G+                 F  P     S LR LS   P  SC    S+++      A+  Y+  + +   LR V  ++  + D++ +E  +       LL     ER  L+K    +  L  VA  L   ++   S+   +P      + +     K  +     +L Q   L      N SG        G  D          ++ L +LQI    C   +G   +    +      Y   A+++       E IR  +    E  H      V +  A   S H         +  L+E  IS      + P  V     ++     L   +  ++ SILIS Q+      +REL  +   AA  +   E+E    +  IR  HD  +++   L L  ++         L  L+  R+    +    +      +   L++++  +    L + ++HH     L   + N F  I   GFC P+   +  G    +   GT              DVS+ IEDEEQ+LG ++++  D   E ++++ E  G +M+ DF+G LEDV                        G+      + +++K+W  ++  N                                            +  +QDE+  E         +A  D             ++  E+  +D              E  + E P+D+  D                   D   ED +E G    +                              D  +    E N  S  +G  ++A +    +E E  +E + GD  +D       EE +  N+    EDK +   +DVS    + N      +    Q D+         E+EG++   + + A    P  EKE        G+S  G  QADK+ E S +    QG  T                                                   ++Q EK      TNP R L D    +K W   L  + D  +DE  P+  +     DA       ++Y   DD   + Q ++G+A + Q + L +    E  A LD       E +      +++   ++Q S     + + P   N+    L    VEK   +        + N + +A+         E       + D   EE   +  +V  WR    D A+A+ELW   E+     A  LCEQ+RL+LEPT A+++ G YRTGKRLNM+++I Y+AS F+KD+IWLRR KP KR Y V+IA+DDS SM ES +  +A E+LAL+  ALS+LEVG I++ SFG    L+  F++P     E GA +LQ FTF+Q +T + NL+        H++   +    T E   L L+ISDG     + VR  +R   E  ++V F I+D    E++SI     V+Y+   GKM+++   Y+  FP ++Y +++++++LP  L+DALRQ+  +
Sbjct: 3756 PIVNSLKIRILELLDQWPE--------HATLQQLVTISDRLAAFSITS-PIAKLLTGVELLLQKSEDWES-YASRDVSIKSHREAIIALIVSWRQLELNCWPKLLAAQDVYHQNAAYKWWFHLYNTIIRSALISNSNNDDINHRTKDLLGTLDQFLQSSPLSEFSTRLELIHTFHSHLRAQVHFKLSAHQQLNEDSRLMHESVADVLDNVYKYYLQFLASANAKLEQLRKPIEKDLKEFVKIASW-----------------KDVNIHALKQSAQRTHRQLHKCIRKYKEVLA----RSMTDVIAAYQQEVPIFQARDEKHYEDHNPAIVQKLAS--SADIWISSLDVTVPQLIDHR----VEEEKERFANLERTWTKLKKYCEEDLVHFHSSTMQMPLEEFVTDIITQIKTFQQETPHTMTEENKSLVKNQKTLKKKALVDFLKQLKRIGVKQRVHVIAQQNQDQGYIFRLPSVALASSLRDLSLQKPPLSCYNWASDELEPLWSKASDYYYRVLARISHLRTVISTNLLSKDVTLQEIEKGAGSTEYLLSLLISERNSLHKMENNLGLLHGVAVQLASIYE---SSKTMSPVHLDTVKAVQLLAHKMHTDKVADSLAQALSLFGIQTNNFSG--------GLRDH---------ERLLKDLQI----CAAAMGRMREEVDFLFTQRYLYPN-AFYNAPIVVTDEVIRT-IEVHHEIFHDIHQKLVNIMHAMPESRH---------IFGLIEAYISNIYTQGNPPNDVTIESLDTTSIIELRDKSNSVIDSILISIQE------LREL--KKSDAASEIADAESEYGMRDSHIRAEHDRVLSIAHQLRLETVAGRSIDLINQLAILMKHRNPNDHQSAILVTSFLQRVYPFLQQHIY-LTHHTLAEFLLHHKSMAKLTFVMCNTFTTIFTKGFCIPEGAAEDSGDGVEDGVQGTXXXXXXX-----XNDVSDQIEDEEQVLGTQNDKQPDP--EKQDMKEEKKGMEMENDFDGQLEDVXXXXXXXXXXXXXXXXXXXXXXXXGDIDDMNPDAVDDKMWGDDSANNLXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVSEEQDEQAPEG--------FAEEDXXXXXXXXXXXXGSKPGEQFNVDIP------------EAETLELPDDMNLD-------------------DMDGEDGEEKGDGGDEFVXXXXXXXXXXXXXXXXXXXXXXXXXXXXDTAD----EENAQSEPLGEENDATIHDAAIEEEKQEETEAGDEKQD---QNHPEEKENLNKPD--EDKEDTAMEDVSQSREQPNNNANTDNDFGVQGDVGKVSASSFGESEGNNKDAHDSTASKNMPQKEKEES------GKSNRGNTQADKKTETSVK---EQGDET---------------------------------------------------EKQQEK------TNPRRSLGDA---LKSWRRRLNDVADAEEDENEPEARQDPESTDAKVDENQSFEYLKNDDDSHDMQ-TMGSAAEDQVKNLGELGAIEEKAPLDPSEYADMEVD------DSVEETNQQLSA----ELTDPSGNNDKGAILSKRMVEKTDGM--------DENEVQAANASHFAHEPLEQE-----EIDRMREE---LEMKVTEWRETGKDGAKARELWQTYENLTHDLAFGLCEQLRLILEPTMATKLKGDYRTGKRLNMKRIIPYIASQFKKDKIWLRRTKPSKRQYQVMIAVDDSKSMSESHSIQLAYETLALISKALSQLEVGDISIISFGERVQLLHPFDQPF--TDEGGASVLQQFTFAQQKTYVKNLVETSIGLLEHARSNHSGPRGT-ELWQLQLIISDGICEDHDAVRALVRRAAEHQIMVIFIIVDN-KAEKDSILATTNVKYKNVGGKMTLQMERYLDSFPFEYYLILRDVNALPEVLSDALRQYFSL 5499          

BLAST of Gcaud8403.t1 vs. uniprot
Match: A0A372RV02_9GLOM (VWFA domain-containing protein n=1 Tax=Rhizophagus sp. MUCL 43196 TaxID=1803374 RepID=A0A372RV02_9GLOM)

HSP 1 Score: 271 bits (693), Expect = 8.960e-70
Identity = 355/1432 (24.79%), Postives = 633/1432 (44.20%), Query Frame = 0

Query: 1291 KIANHLYFSGIHQYQTLRNVSDSSTRNNDISAEEAGRIRAFCRDLLDKSAMERTELNKCSAIVHCLSKVADALTKGHQDILSTSRENPAFFGCFRDILFRLRKAKSTLQQISKLMSEAQRNMSGDADF--VQGQGTLDEVLCFYRKVGKKSLSELQIILNTCCDKIGNQPKAFSLMDIDHVEYQELAYFDGSYKEWCETIRAYLSSLKETLHV--GLSR-AREVSEH-NLVTEILSPLLSLVEDGISE---------ESPLPSSPAQVASALYNSLLSNAEELVHSILISSQKAMAWNGVRELLGENKSAAPPLGKDETELFPEGVIRRGHDGTVNLGSYLLLTRISSHFDANSRFLQSLIPLRDVLAWKETKELIGIQNALGSILKE---YLRS--MLMPCLEKAIVHHHENLALLR-TLTNLFIGICKDGFCRPDLEEQTEGMMETEVKS-GTGFGDVGNGDMSGAQDVSEAIEDEEQLLGLRDE-RDKDLRDENENVRNEDGFDMKTDFEGTLEDVDSEGVQDMEGDG---EAEKQMGQEHGKGENLLNEKLWDSENETNKEQASELDSKQDEEESESQGNGKQGEWAVGDRRDRSHSKEMNEQNREEEKSELDASRVXXXXXXNAEEELGSYEEPEDLEKDASRDREHGDHRENGEQENCDTHSEDIQESGTEKQKKHGEEDHGMTDGYNSDFARGQE---PDPCLPESDQPEVGEAE-SNCGSPMVGVGDEAEVLEYEGMDEDQTGDFHEDCQ--LSGLEEEADMGNEKTNPEDKLEHVKKDVSPDLAKTNEEEEAKSISSAQDDIKSDGGEHCQETEGDSDVLNGADADAKQPVSEKEHRSMNILPGRSTTGQADKEIEGSTEPFAHQGQGTLGFGSNMNDADGERNDIADRSSHDHQPGPGNQINKQNGEEEGRKLESSMKQDEQLE---KGSSSMNTNPLR-LSDPEHLIKEWNECLRALRDDDEQLPKQTEG------ENDADAWQYETIDDPKENPQHSLGAATQKQQRPLPDERVAELDSESIRHKEAEPKPKLREAMSTQDRQKSHFRQEQSSSPQNPNESLGDLVHGDVEKPHPVRSAIDRERERNRIASAHIGQSESSIFETRGKPGCDEDERNEEPTPVTSQVLGWRMENLDHAQAKELWHALESKVASEAATLCEQMRLVLEPTKASRMGGGYRTGKRLNMRKVIEYVASDFRKDRIWLRRVKPDKRSYDVLIAIDDSASMLESEAGVMATESLALVISALSKLEVGRIAVASFGSTANLIRHFEEPLPINTESGAKLLQHFTFSQTETNIVNLL--SFIHSQMGSTNNESTVEHISLALVISDGRLSHREEVRRQLRILKESNVLVAFFILDRGTVEENSIYNVQRVEYEA-NGKMSVRP--YMHDFPIDFYAVVQNMSSLPGTLADALRQWIEI 2675
            K A+  Y+  I +   LRNV+ S ++  D++++E  +   F  DLL     ER  +    A+     ++   L +  +   S + ++     C  + L      + T+    +++ +    +        +Q Q      L  Y ++G K        L+   DKI    K     D  H+  +E+   + +  +     +  ++ +K+ + +  GL + AR+ S      + IL+P+   +++ I           E  L S   Q  + L N +     +L+ ++L++ Q             +  SA P   +D  +  P+G IR+ H+  +NLG +L +  +   F       + LI L D     E    +  QN + ++L+    +++   +++     + ++HH++L  L   L N F  +   G+C P +E   + + + E  + GTG G+       G +DVSE IEDEEQ+LG ++E +D D  ++   ++ + G +++ DFEGT+EDV+                E++MGQ      + ++EK+W              DS  D EES    N KQ   A G         E +   +E++++    S+       N E ++    + + +E +   D    D   N E +N D                        +D +N D  + +    P+  + +  +    E E +N   P     DE+EV   + MD  Q G F+E+    +   E + D  + +T   +K+   K + + +++  N++E +K       ++  +   H  +   D ++L   + + +   +  + +  +  P  +  G     ++G +         + G GS  +  + E  D  + S          Q  K  GE      +   K +  +E   K  S+ + NP R L D    +K+W   L  +    E + +Q          N+   +++   DD   + Q ++GAA + Q        +  +D+   +H   E    + E     D  ++      +    N     G ++   + K    +   D +     I S+H      +  E        E  R E    +  ++  WR    D  +A+ELW   E+     A  LCEQ+RL+LEPT A+++ G YRTGKRLNM+K+I Y+ASDF+KD+IWLRR KP KR Y V+IA+DDS SM E+ +  +A E+LAL+  ALS+LEVG +++ SFG    L+  F+ P   ++E+GA++LQ FTF Q +T + +L+  S    +    N     E   L L+ISDG     E ++  +R   E+ ++V F I+D    ++ SI ++  V+Y++ NG+M++    Y+  FP D+Y V++++++L  TLADALRQ+  I
Sbjct:   26 KKADEYYYKIIARMIHLRNVAASYSK--DLTSQEVQKGLGFSEDLLYLLIQERKNIQ---ALDKQYVRLLGVLIQFSRIYSSHNAQHSYTKVC--EYLSFCSIDERTIWNFKEILDDLNSLLFHSCVIFDIQKQNEQSNYLSHYEEIGTK--------LHDWLDKISTVRK-----DFVHLFDREILLPEYTIGKKALITKDIINLVKDNIVLIDGLYQFARDYSSRIKCFSHILAPICDYIKNCIGNLYLTETVHNEMNLNS---QTTTVLGN-IAKKVNDLIDAVLVAIQAL-----------QKSSAEPTKDEDIDDDIPDGYIRQEHEHILNLGKHLRIPLVLERFIILH---EQLINLMD----DERFNNLDCQNLISNLLQRIFPFIQQYYLVVQYYLNSFIYHHKSLCKLTYVLCNSFATVFMKGYCMPQMESGDDEIGDIEENAQGTGIGEG-----EGTKDVSEEIEDEEQVLGNQNEMKDNDSANDRLKLKKDKGIEIENDFEGTIEDVEXXXXXXXXXXXXXENIEQEMGQFDDNDPDAVDEKMWGD------------DSADDVEESRKTTNTKQESGAQG---------ETDIVAKEDQETTTQGSQEKNIE--NGEMDVEGDSQQDSMETEYQSD---DDDNINEEYDNFD-----------------------KSDHFNVDIPQAETLELPEDMMIDDIEGNTNEEEINNDDLPSNMDIDESEVQTKDTMDNTQDG-FNEEAYDAMETDEIDDDSVSNETKDGNKMGVEKIENNDEISDENQQESSKG------EVPKERPNHKVQLSTDDNLLEDMNIENEDQKTSNQEQPNSDAPAENLFG-----VDGESGKANMASSKSSGNGSEQSLQETENQDSNNNS----------QRYKSKGESXXXXXDELKKSENTIENQSKDQSTKDANPHRSLGDA---LKQWRRRLDNIVKGGENISEQENNLEQNQIVNEDQTFEFLENDDDSHDLQ-TMGAAAEDQ--------IGAIDNNE-QHYNYETNYAVEENEIDYDNSQNDDALPMNDYDHNSENEKGAILSRKIVKQDDEQQLFDEKFNPLSIYSSHFTHEPLTREEI-------ESLRQE----LEIKLTDWRQSGRDITKARELWQKYENLTYDLANGLCEQLRLILEPTLATKLKGDYRTGKRLNMKKIIPYIASDFKKDKIWLRRTKPSKRQYQVMIAVDDSKSMCETHSIQLAYETLALISKALSQLEVGDLSIVSFGEKVQLLHSFDRPF--SSEAGAQVLQQFTFEQNKTYVRSLMETSITLLEHAKNNGTKNKELWQLQLIISDGVCEDHESLKALVRKAAEARIMVVFIIVDNKPGKD-SIMSMNHVKYKSINGRMTIHMERYLDTFPFDYYVVLRDINALSETLADALRQYFSI 1312          

BLAST of Gcaud8403.t1 vs. uniprot
Match: A0A8H7PPM8_9FUNG (Midasin n=1 Tax=Umbelopsis vinacea TaxID=44442 RepID=A0A8H7PPM8_9FUNG)

HSP 1 Score: 272 bits (696), Expect = 4.290e-69
Identity = 461/1910 (24.14%), Postives = 791/1910 (41.41%), Query Frame = 0

Query:  866 NHPVLDGIMVAIDSVARVSKSSTPLSIVVIGLENVLRKADEWNRLFATGETKLRAELVEVSRLVSRWRGVEMRSWPLLLAARKREFETQADKWVFLIYDAVINETLSAGMHDNEQVNSA--LSTIDRFLRSSPSGEFCRRLAIVQSISHHLLSLNDARRDLAQFIGNS-------LRGICRFYHLFKDHLEQKLSQRYETVLQELKEFTNIVSYNPQEELGSAVEMSKGRDKNLDYFRLKALSDKTRRRVHKLCLKVDGIL-------------RVPFYEH-----MMDFTAGF------GFDHPIILN-----GASKHDPVRPLGQDILTKTSRGIACDERDVLDSKHRSSILLPAALKMLSRFDTLRTRLVTYGREIQSSSRTECEAASAFSSKLRTMIRAR--VSKLREMR-LGSDIQPKRRALID----LLKGLSRAGLSPF--ERPHANSMLRWLSTSDPSSCEVSNKIANHLYFSGIHQYQTLRNVSDSSTRNNDISAEEAGR-------IRAFCRDLLDKSAMERTELNKCSAIVHCLSKVADALTKGHQDILSTSRENPAFFGCFRDILFRLRKAKSTLQQISKLMSEAQRNMSGDADFVQG----QGTLDEVLCFYRKVGKKSLSELQIILNTCCDKIGNQPKAFSLMDIDHVEYQELAYFDGSYKEWCETIRAYLSSLKETLHVGLSRAREVSEHN-LVTEILSPLLSLVEDGISEESPLPSSPAQVASALYNSLLSNAEELVH----SILISSQKAMAWNGVRELLGENKSAAPPLGKDETEL-FPEGVIRRGHDGTVNLGSYLLLTRISSHFDANSRFLQSLIPLRDVLAWKETKELIGIQNALGSILKEYLRSMLMP--CLEKAIVHHHENLALLRTLTNLFIGICKDGFCRPD-LEEQTEGMMETEVKSGTGFGDVGNGDMSGAQDVSEAIEDEEQLLGLRDERDKDLRDENENVRNE-DGFDMKTDFEGTLEDVDSEGVQD-------------------------MEGDGEAEKQMGQEHGKGENLLNEKLWDSENETNKEQASELDSKQDEEESESQGNGKQGEWAVGDRRDRSHSKEMNEQNREEEKSELDASRVXXXXXXNAEEELGSYEEPE----DLEKDASRDREHGDHRENGEQENCDTHSEDIQESGTEKQKKHGEEDHGMTDGYNSDFARGQEPDPCLPESDQPEVGEAESNCGSPMVGVGDEAEVLEYEGMDED-QTGDFHEDCQLSGLEEEADMGNEKTNPEDKLEHVKKDVSPDLAKTNEEEEAKSISSAQDDIKSDGGEHCQETEGDSDVLNGADADAKQPVSEKEHRSMNILPGRSTTGQADKEIEGSTEPFAHQGQGTLGFGSNMNDADGERNDIADRSSHDHQPGPGNQINKQNGEEEGRKLESSMKQDEQLEKGSSSMNTNPLRLSDPEHLIKEWNECLRALRDDDEQLPKQTEGENDADAWQYETIDDPKENPQHSLGAATQKQQRPLPDERVAELDSESIRHKEAEPKPKLREAMSTQDRQKSHFRQEQSSSPQNPNESLGDLVHGDVEKPHPVRSAIDRERERNRIASAHIGQSESSIFETRGKPGCDEDERNEEPTPVTSQVLGWRMENLDHAQAKELWHALESKVASEAATLCEQMRLVLEPTKASRMGGGYRTGKRLNMRKVIEYVASDFRKDRIWLRRVKPDKRSYDVLIAIDDSASMLESEAGVMATESLALVISALSKLEVGRIAVASFGSTANLIRHFEEPLPINTESGAKLLQHFTFSQTETNIVNLL--SFIHSQMGSTNNESTVEHISLALVISDGRLSHREEVRRQLRILKESNVLVAFFILDRGTVEENSIYNVQRVEYE-ANGKMSVR--PYMHDFPIDFYAVVQNMSSLPGTLADALRQW 2672
            +H VL  ++V ID +      S P++ ++ G+E +L+K+++W   +A+ +  ++     +  L+ RWR +E+  WP LLAA+++  +  A KW F +YD VI+ ++  G  D ++ N+   LS +D FL+SSP GEF  RL +V S   HL ++     D A   G +       L  +  +Y  F   ++  L +  + + ++LK+F  I S+                 K+++ + L+  + KT  ++HK   K   +L              +  Y++       D   GF        D  ++ +     G  +  P +   ++I    +R         L S  +S IL   +L      ++  T ++   +  Q  +  E    +    KL+ + + +  V  L+++R LG  I+    A  +    L+    +A LS    +R   +S L ++S + P   ++ +K AN  YFS + +   LR+VS  +  + DIS  E  R       + AF R   ++SA+ R EL+  S +     ++A A ++   + L  S               RL   K +  ++  ++S+    +S  + +       Q  L ++L   R V K         +N    ++   P++   +D                +   + +  Y  +L+       SR  E+S+    V ++L P++  +E+    +  + S+ A V        L++ ++ VH    +IL S Q       +R L  + + A     +D+ E   PE  IR  H   +N  + L       H D   + L  L+    +    E+ +L      L  +     + ML+    L + ++HH     L   L N F  I K GFC P+ + +  EG    +   GTG   VG G   G +DVS+ IEDEEQ+LGL++E +   +DE ++ + E +G +M+ DFEG LED++                               ++ D   +K  G +  +G N  ++K  D +    ++Q S++ +K  EE   + G  ++ E             +  E   +EE  E +    XXXXX  A E++ + E PE    DL  D   D + GD ++  E+       ED  ++        GEE+  M +   +   +  E +P     + P                                  GD  E                 T P+                 +E  EA +I+  Q  I+                  G+   A    + ++                                                    D S  + Q   G+ +     E   R+L   M  +E                 + + +  + N     +++D++    QT G    D  +           Q  LGA  +    P  +++VA          EA+P  +L +    +D              QN N + G +++    K +P  S I  E +        I  ++ S+         D ++  EE   + ++V  WR +  D   ++ELW   E+     A  LCEQ+RL+LEPT A+++ G YRTGKRLNM+K+I Y+AS F+KD+IWLRR KP KR Y V+I++DDS SM ES +  +A E+LAL+  ALS+LEVG IA+ SFG    L+  F++P     ESGA +L+ FTF Q +T +  L+  S    +    N+    +   L L+ISDG     E ++  +R   E  +++ F ++D    E++SI N   V+Y   NGKMS++  PY+  FP  ++ +++++++LP  L+DALRQ+
Sbjct: 3734 DHAVLQQLIVIIDRLVGFPIVS-PIAKLLTGIELLLQKSEDWEA-YASRDVSIKEHRDAIISLIVRWRQLELNCWPKLLAAQEQYSQDGAYKWWFHLYDTVISTSIQTGSTDEDKENTKNLLSVMDHFLQSSPIGEFKTRLNMVDSFRKHL-NVESHFTDAAGGSGANYAALSSLLSNVHDYYSQFSVSVQAMLERLRKPIEKQLKDFVKIASW-----------------KDVNIYALRQSAQKTHTQLHKCIRKYKDVLMRSTLDVIGVYNEEIAMYQYGDEKKYGDVNLGFVNQLASASDSWLVKSKFPTAGLIEQSPAKDRFKNIDRTFNR---------LQSYFKSDILPVDSLLANLPLESFLTEVIQQIKTFQKETPAELTDENKSQVKLQKVFKKKSLVDFLKQLRRLGLKIRVPTLAEQNQDNTLVFRQRKAQLSMVLQDRDLLDSSLSYISWAAPDLIKLWDK-ANTYYFSSVARISHLRSVSKVNV-HTDISLTEVERSVSATEHMFAFIRQ--ERSALSRAELSM-SLVEGITVQLAAACSQHPLEALEDSE--------------RLTVKKMSADKLVDVISQTIELLSLQSTYGSSNTVLQIELGQLLQEARTVHKD--------INEIYKRVYLYPRSTHSVDT--------ILMSSDVQAKIQDVDTYFKTLQ-------SRLIEISQDTPSVYQVLLPIVQHLEEASKNQPTVVSTDAAVD-------LTSVQDQVHGTIDAILTSIQD------MRNLASKTEYA-----EDDLEYGMPENHIRDQHAAQINRLNAL-------HLDVLGKRLLELLKTLSLYNQTESADLNSASILLQQLYPFVQQHMLIVQRTLCEVLLHHKAMAKLTYVLINSFTLIMKKGFCLPEGMADDEEGDGMDDNVQGTG---VGEG--QGNKDVSDQIEDEEQVLGLQNEVEP-AKDEKKDTKEEKNGLEMENDFEGDLEDIEXXXXXXXXXXXXXXXXXXXXEMDDQVXXXXXLDPDAVDDKMWGDDSAEGLNE-SDKTIDEDKSNGEQQDSDIVAK--EEPDSADGKDQKSEXXXXXXXXXXXXGDGKEPEIDEEGEEGEDXXXXXXXXNKAGEQMNA-EIPEAETLDLPDDLMMDGDEGDEQDGEEK------GEDFNDAMDLDDDHKGEEEEMMDEDGEAFKDKLDEAEPEAEGEEMPXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXNAGDHQEXXXXXXXXXXXXXXXXXTGPD-----------------HEAPEADTIADNQFGIQ------------------GSSGKASSSAAGQQEGXXXXXXXXXXXXXXXXXXXXXQGXXXXXXXXXXXXXXXXXXXXXXXXXXXDPSKPNPQRSLGDAL-----ENWKRRLADVMDAEEXX---XXXXXXXXXXXEESDDIEVQDNHAFEYIKNDEDAHDMQTMGSAAQDQLK-----------QMDLGAMDETV--PESEDQVAXXXXXXXXXXEAKPIEQLDQYPIDKD--------------QNDN-AKGAILN----KRNPQDSKIPEESD--------ILTTDGSVVAHEPLDEQDIEQLREE---LENKVSQWRDQGRDEQTSRELWQQYENITHDLALGLCEQLRLILEPTLATKLKGDYRTGKRLNMKKIIPYIASQFKKDKIWLRRTKPSKRQYQVMISVDDSKSMSESHSVQLAYETLALISKALSQLEVGDIAITSFGERIQLLHPFDQPF--TDESGASVLRQFTFGQDKTYVKKLVESSIALLENAKNNHSGNGDLWQLQLIISDGICEDHEALKALVREAAEHQIMIIFIVVDNKP-EKDSILNTTNVKYSMVNGKMSLQMTPYLESFPFQYFLILRDINALPEVLSDALRQY 5442