Gcaud3165.t1 (polypeptide) Gracilaria caudata M_176_S67 male

BLAST of Gcaud3165.t1 vs. uniprot
Match: A0A2V3J6P6_9FLOR (Alpha,alpha-trehalase n=1 Tax=Gracilariopsis chorda TaxID=448386 RepID=A0A2V3J6P6_9FLOR)

HSP 1 Score: 811 bits (2096), Expect = 2.030e-291
Identity = 375/469 (79.96%), Postives = 420/469 (89.55%), Query Frame = 0

Query:   15 IATAIASLCPAVTDPTQHQFITDAKAVLDGNWVPTMNSTLPSPNLYPHQWSWDSSFISIGYSHYDTQKAIDETDALFRAQWRNGLLPHIVFNPNADESYFPGPSFWKIETSNSYPLNSHTSGIVQPPVHAIASLAIFNNARSAEQKKLALEHLSTIYPKLVNWHNYLYGERDPLNENLVYIRHMWESGMDNSPAWDDALNSIQLTADQIPQYTRVDKGKVGDHHERPSAHFYDRAVHLIKIFYDNQYDESAIFAKSPFIIQDVLFNSILAHAGDALADIAHILGKNEEEERHRIQAARTAEAISSKLYHEEDGFFYDYDMKSEKLIRTKISGGLVGVYGAKMDSAKLKSILGHLREPGFLGPDLSGFTIPSVSRDDPGYTNTTYWKGPAWININYLVRDGLLRNANGNQDAEEIASYLKERSMDMLKTSGFYEYFNPISGSPHGGHQFSWSAALTIDWMCNASTSSDEV 483
            ++ ++ASLCPAVTDPTQ  F+T AKAVLDGNWV +MNSTLPSPNLYPHQWSWDS+FI++GYSHYDT KAI ETDALFRAQWRNGLLPHIVFNPNADESYFPGP+FWKI TS ++PLNS TSGIVQPPVHAIASLAI+NNA+SA+QKKLA+ HL ++YPKLVNWHNYLY ERDPL ENLVYIRHMWESGMDNSPAWDDALN+IQLT DQIPQYTRVDKGKVG HHERP++HFYDRAV+LIK+FYDN YDE AIF  SPF+IQDVLFNSILA AG+ALADIAHILGK +    HR Q+A+TA AISSKLYH EDGF+YDYDM ++KLIRTKISGGLV +YGAK+DS  L SI+ HL +P FLGPDLS +TIPSV+RDDPGYTNTTYWKGPAWININYLVRDGLLRN NGN+DA +IA +LK RSM ML  +GFYEYFNPISGSPHGGHQFSWSAALTIDWMC+ S+ +  +
Sbjct:   17 VSASLASLCPAVTDPTQQAFVTSAKAVLDGNWVTSMNSTLPSPNLYPHQWSWDSAFIALGYSHYDTHKAIAETDALFRAQWRNGLLPHIVFNPNADESYFPGPAFWKIHTSPAHPLNSQTSGIVQPPVHAIASLAIYNNAKSAQQKKLAMHHLQSVYPKLVNWHNYLYNERDPLKENLVYIRHMWESGMDNSPAWDDALNAIQLTPDQIPQYTRVDKGKVGSHHERPNSHFYDRAVYLIKVFYDNSYDEQAIFKSSPFLIQDVLFNSILARAGEALADIAHILGKKQAAVHHREQSAKTAAAISSKLYHAEDGFYYDYDMVAKKLIRTKISGGLVALYGAKVDSTHLNSIVNHLYDPAFLGPDLSSYTIPSVARDDPGYTNTTYWKGPAWININYLVRDGLLRNGNGNEDAVKIAKFLKTRSMSMLGAAGFYEYFNPISGSPHGGHQFSWSAALTIDWMCSTSSETSPI 485          

BLAST of Gcaud3165.t1 vs. uniprot
Match: R7QIP8_CHOCR (Glyco_hydro_63 domain-containing protein n=1 Tax=Chondrus crispus TaxID=2769 RepID=R7QIP8_CHOCR)

HSP 1 Score: 637 bits (1643), Expect = 8.580e-223
Identity = 298/451 (66.08%), Postives = 354/451 (78.49%), Query Frame = 0

Query:   32 HQFITDAKAVLDGNWVPTMNSTLPSPNLYPHQWSWDSSFISIGYSHYDTQKAIDETDALFRAQWRNGLLPHIVFNPNADESYFPGPSFWKIETSNSYPLNSHTSGIVQPPVHAIASLAIFNNARSAEQKKLALEHLSTIYPKLVNWHNYLYGERDPLNENLVYIRHMWESGMDNSPAWDDALNSIQLTADQIPQYTRVDKGKVGDHHERPSAHFYDRAVHLIKIFYDNQYDESAIFAKSPFIIQDVLFNSILAHAGDALADIAHILGKNEEEERHRIQAARTAEAISSKLYHEEDGFFYDYDMKSEKLIRTKISGGLVGVYGAKMDSAKLKSILGHLREPGFLGPDLSGFTIPSVSRDDPGYTNTTYWKGPAWININYLVRDGLLRNANGNQDAEEIASYLKERSMDMLKTSGFYEYFNPISGSPHGGHQFSWSAALTIDWMCNASTSSDE 482
            H  +  A+AVL  NWV  MNSTLPSP+LYPHQWSWDS+F++IGYSHYDT +AI E  ALFRAQW NGLLPHIVFNP  DESYFPGPSFW    S   P  + TSGIVQPPVHAIASLA+++NA+    K LA  HL++IYP LV WH YLY ERDPL E LV++RHMWESGMDNSPAWD+ L ++ +T D IP Y+RVDKGKV    ERP++ FYDRAV+LIKIFYDN YDE AIF KSPF++QDVLFNSIL+ AG ALA+IA IL KNEE + HR +A +TA+AI+ KLY   D F+Y+Y+M ++KLIR KISGG    YGA MD   + +++ HL  P FL  DL  +TIPSV++ DP YTN+TYWKGPAW+NINY+VR+GLL NA GN+DA  IA+YLKERSM+M++ SGFYEYFNPISGSPHGGHQFSWSAAL IDW+C  + S  E
Sbjct:   28 HPLLAPAQAVLSANWVSDMNSTLPSPSLYPHQWSWDSAFVAIGYSHYDTPRAIAELHALFRAQWANGLLPHIVFNPATDESYFPGPSFWNTHRSPHAPPAAATSGIVQPPVHAIASLAVYHNAKGPHMKALAHRHLTSIYPNLVAWHRYLYAERDPLQEGLVFLRHMWESGMDNSPAWDEPLAAMHVTPDAIPTYSRVDKGKVAHAKERPTSFFYDRAVYLIKIFYDNAYDEKAIFEKSPFLVQDVLFNSILSRAGMALAEIADILHKNEEADAHRQRAEKTAKAITDKLYDPVDKFYYNYNMVTKKLIRRKISGGFGAFYGANMDDEHVNALIDHLYSPDFLEHDLYAWTIPSVAKTDPSYTNSTYWKGPAWLNINYIVREGLLANAAGNRDAVRIAAYLKERSMEMIEKSGFYEYFNPISGSPHGGHQFSWSAALIIDWICTTNRSYGE 478          

BLAST of Gcaud3165.t1 vs. uniprot
Match: A0A7S3EES9_9RHOD (Alpha,alpha-trehalase n=2 Tax=Rhodosorus marinus TaxID=101924 RepID=A0A7S3EES9_9RHOD)

HSP 1 Score: 435 bits (1119), Expect = 6.020e-144
Identity = 209/448 (46.65%), Postives = 295/448 (65.85%), Query Frame = 0

Query:   31 QHQFITDAKAVLDGNWVPTMNSTLPSPNLYPHQWSWDSSFISIGYSHYDTQKAIDETDALFRAQWRNGLLPHIVFNPNADESYFPGPSFWKIETSNSYPLNSHTSGIVQPPVHAIASLAIFNNARSAEQKKLALEHLSTIYPKLVNWHNYLYGERDPLNENLVYIRHMWESGMDNSPAWDDALNSIQLTADQIPQYTRVD-KGKVGDHHERPSAHFYDRAVHLIKIFYDNQYDESAIFAKSPFIIQDVLFNSILAHAGDALADIAHILGKNEEEERHRIQAARTAEAISSKLYHEEDGFFYDYDMKSEKLIRTKISGGLVGVYGAKMDSAKLKSILGHLREPGFLGP--DLSGFTIPSVSRDDPGYTNTTYWKGPAWININYLVRDGLLRNANGNQDAEEIASYLKERSMDMLKTSGFYEYFNPISGSPHGGHQFSWSAALTIDWMCN 475
            + + ++ A+ VL+ NW+P MNSTLPS NLYPHQWSWDS+FI+ GY+HYD  KA +E  +LFRAQW+NGL+PHIVFNP    SYFPGP +                 IVQP VHAIA L  +  +         +E+L  ++P L  WH+YLY ERD   E+LV++RH WE+GMDN+P+WDDA N I+L    +P Y R D K    D  +RP+  +YDR V+L+KIFYDN YDE+ I AK P++IQDVLFN+IL+ A D LADIA ++GK   E R R +  RTA  I  K+++ ED  FYDYD+ S+++I+ +I G + G+YG  +    +K+ L  L     +G   + +GF +P++  ++  Y    YW+GP WININY+V +GLL  + G ++A E A YL ++++D+++  GF+EYF+ +  SPHGG  FSW+A+L +D +C+
Sbjct:   36 EKELLSGARKVLNVNWMPEMNSTLPSANLYPHQWSWDSAFIAFGYAHYDIVKAKEEMRSLFRAQWKNGLVPHIVFNPQTSGSYFPGPKYXXXXXXXXXXXXXXXXXIVQPAVHAIAVLKTYEASGD-------IEYLKEMFPLLKKWHDYLYRERDIHGEHLVFVRHPWETGMDNAPSWDDAFNRIELKEGDVPPYKRADNKHSDVDASDRPTDFYYDRYVYLMKIFYDNNYDEAQIAAKCPYLIQDVLFNTILSKAEDTLADIAVLIGKPSAEFRTRAEKTRTA--ILEKMWNAEDAAFYDYDLYSDEVIKKRIGGAMAGIYGVTLPKNDMKNFLAVLDSEEAMGRLGNQTGFPLPTMLTNEETYMPNNYWRGPVWININYMVIEGLL--SYGTEEALETAQYLFKKTLDLVQVGGFFEYFSALDASPHGGGDFSWTASLVVDMICD 472          

BLAST of Gcaud3165.t1 vs. uniprot
Match: A0A3M1ZSR0_9CHLR (Glycoside hydrolase n=1 Tax=Chloroflexi bacterium TaxID=2026724 RepID=A0A3M1ZSR0_9CHLR)

HSP 1 Score: 388 bits (996), Expect = 9.330e-127
Identity = 197/439 (44.87%), Postives = 273/439 (62.19%), Query Frame = 0

Query:   35 ITDAKAVLDGNWVPTMNSTLPSPNLYPHQWSWDSSFISIGYSHYDTQKAIDETDALFRAQWRNGLLPHIVFN-PNADESYFPGPSFWKIETSNSYPLNSHTSGIVQPPVHAIASLAIFNNARSAEQKKLALEHLSTIYPKLVNWHNYLYGERDPLNENLVYIRHMWESGMDNSPAWDDALNSIQLTADQIPQYTRVDKGKVGDHHERPSAHFYDRAVHLIKIFYDNQYDESAIFAKSPFIIQDVLFNSILAHAGDALADIAHILGKNEEEERHRIQ-AARTAEAISSKLYHEEDGFFYDYDMKSEKLIRTKISGGLVGVYGAKMDSAKLKSILGHLREPGFLGPDLSGFTIPSVSRDDPGYTNTTYWKGPAWININYLVRDGLLRNANGNQDAEEIASYLKERSMDMLKTSGFYEYFNPISGSPHGGHQFSWSAALTID 471
            +  A+AVL  NW  T   T PSP LYP+QWSWDS++I+IGY+HYD ++A  E   LF  QWRNGLLPHI+FN       YFPGP FW+   S   P    TSGIVQPP+HA A L ++ +A   EQ +     L+ I+PKL  WH YLY ERDP  E LVYIRH WESG DNSP WD+AL  ++L ++QIP Y RVD   V D  ERP+   YDR ++L+++F +  YDE+AI+   PF++QDV+FN++L  A   LA+IAHILG   E+ R  I+ A +T  AI+ KL+ + D  +YDYD+ S++LI      G + ++    D  + +++ GHL    F     SG+ +PS  +  P Y    YW+GP W+N+++L+  GL      +   +  A  L    +++ K  GF+EYF+P +G+ +G + FSW+AAL ID
Sbjct:   10 VEQAQAVLQRNW--TGRFTKPSPRLYPYQWSWDSAYIAIGYAHYDLERAQKELRTLFAGQWRNGLLPHIIFNFSRGPRVYFPGPDFWQTRRSPHAPKRRRTSGIVQPPIHATAVLRVYQSANDGEQVR---PFLAEIFPKLKAWHEYLYRERDPYQEGLVYIRHPWESGQDNSPIWDNALARMELKSEQIPAYRRVDTQTV-DAAERPTDSEYDRYIYLVRMFSERNYDEAAIWPDCPFLVQDVVFNTLLCQANRDLAEIAHILG---EDGRLFIEWADQTGYAINQKLWSKTDRIYYDYDLNSKELITEPAVAGFIPLFAHIPDQTRAQNMYGHLNSDSFWKMGDSGYPVPSYDKHAPKYLPNRYWRGPVWMNMDWLLAQGL-----EHYGYQAYADALWHTIVELPKHHGFFEYFDPRTGTGYGAYDFSWTAALLID 434          

BLAST of Gcaud3165.t1 vs. uniprot
Match: A0A6J4QN77_9ACTN (GH63 / GH37 n=1 Tax=uncultured Rubrobacteraceae bacterium TaxID=349277 RepID=A0A6J4QN77_9ACTN)

HSP 1 Score: 383 bits (984), Expect = 5.400e-125
Identity = 195/456 (42.76%), Postives = 280/456 (61.40%), Query Frame = 0

Query:   26 VTDPTQHQFITDAKAVLDGNWVPTMNSTLPSPNLYPHQWSWDSSFISIGYSHYDTQKAIDETDALFRAQWRNGLLPHIVFNPNADESYFPGPSFWKIETSNSYPLNSHTSGIVQPPVHAIASLAIFNNARSAEQKKLALEHLSTIYPKLVNWHNYLYGERDPLNENLVYIRHMWESGMDNSPAWDDALNSIQLTADQIPQYTRVDKGKVGDHHERPSAHFYDRAVHLIKIFYDNQYDESAIFAKSPFIIQDVLFNSILAHAGDALADIAHILGKNEEEERHRIQAARTAEAISSKLYHEEDGFFYDYDMKSEKLIRTKISGGLVGVYGAKMDSAKLKSILGHLREPGFLGPDLSGFTIPSVSRDDPGYTNTTYWKGPAWININYLVRDGLLRNANGNQDAEEIASYLKERSMDMLKTSGFYEYFNPISGSPHGGHQFSWSAALTIDWMCNASTSSD 481
            +TD      I  AK VLD N V +   T P P LYPHQWSWDS FI++GY+HYD  +A++E + LF +QW+NGLLP IVFNP+  E YFPG  FW  + S + PL+  TSG+VQPP+HA A+L ++   R A+ +  A + L   +P L  WH YLY +RDP    L YIRH WESGMDNSP WD+ +  + L  D+IP Y R D   V    +RP    YDR   L+++F ++ YDE+ I    PF++QDVLFN++L  AG  LA+IA +LG  E+      QA +TA AI+ +L+ EE   + DYD+ +++ IR  ++ G   +Y    D  + + +LG L   GF       +++PS  R   G++   YW+GP W+NIN+L+  GL R      DA+  A +L++ ++D+++  GFYEYF+P  G+ HG   FSW+AAL +D + +A+  ++
Sbjct:    1 MTDSNLALLIRRAKMVLDFNRVESY--TQPGPRLYPHQWSWDSGFIAMGYAHYDQDRAVNELNHLFDSQWKNGLLPQIVFNPDFSE-YFPGVGFWHADRSPNAPLHHKTSGVVQPPIHATAALHVY---RHAQNEARARKFLERAFPHLAAWHEYLYRDRDPEGTGLAYIRHPWESGMDNSPMWDETMMRMHLRPDEIPPYQRADTHLVAVD-DRPVNAAYDRFAWLVQLFAEHNYDEARIREDCPFLVQDVLFNTLLCKAGHDLAEIARVLG--EDPSTFEAQALQTASAINERLWDEERAIYMDYDIVTDEPIRVFVAAGFTPLYAGIPDEDQTRRLLGTLENSGFSFGAKGCYSVPSYDRYGYGFSPVQYWRGPVWVNINWLLLRGLERYG---LDAQ--ADHLRQTTIDLVREGGFYEYFHPTRGNGHGSDFFSWTAALLLDILLDATPKTN 442          

BLAST of Gcaud3165.t1 vs. uniprot
Match: A0A2N5K6B0_9CHLR (Glycoside hydrolase n=1 Tax=Chloroflexi bacterium TaxID=2026724 RepID=A0A2N5K6B0_9CHLR)

HSP 1 Score: 380 bits (976), Expect = 4.200e-124
Identity = 199/431 (46.17%), Postives = 267/431 (61.95%), Query Frame = 0

Query:   33 QFITDAKAVLDGNWVPTMNSTLPSPNLYPHQWSWDSSFISIGYSHYDTQKAIDETDALFRAQWRNGLLPHIVFNPNADESYFPGPSFWKIETSNSYPLNSHTSGIVQPPVHAIASLAIFNNARSAEQKKLALEHLSTIYPKLVNWHNYLYGERDPLNENLVYIRHMWESGMDNSPAWDDALNSIQLTADQIPQYTRVDKGKVGDHHERPSAHFYDRAVHLIKIFYDNQYDESAIFAKSPFIIQDVLFNSILAHAGDALADIAHILGKNEEEERHRIQAARTAEAISSKLYHEEDGFFYDYDMKSEKLIRTKISGGLVGVYGAKMDSAKLKSILGHLREPGFLGPDLSGFTIPSVSRDDPGYTNTTYWKGPAWININYLVRDGLLRNANGNQDAEEIASYLKERSMDMLKTSGFYEYFNPISGSPHGGHQFS 463
            Q +  AKAVL  NWV     T P+P LYPHQWSWDS+FI+IGY+H+D  +A  E  +LF AQW NGLLPHIVFNP A   YFPGP  W+IETS   P    TSGIVQPPVHA A L +F +A   +Q +     L+ ++P L  WH YLY ERDP NE LVYIRH WESG DNSP WD     + L  DQIP Y R D   V D  +RP+   YDR V+L+K+F D+ YDE+ I A  PF+IQDVLFN++L  +   LA+IA ILG  E+       A RT++AI+ KL++E+ G + +YD+   + I   ++ G   ++    +  + + +  +L  P F   D   + +PS +R  PGY+   YW+GP WIN+++L+  GL R        +E A +L++  +++++  GFYEYFNP +G  HG  QFS
Sbjct:    7 QLVEQAKAVLHENWVGGF--TKPAPRLYPHQWSWDSAFIAIGYAHHDQGRAEQELRSLFDAQWTNGLLPHIVFNPQAT-GYFPGPELWRIETSPHAP-QQRTSGIVQPPVHATAVLHVFRHAHDHDQGRA---FLAEMFPGLRAWHAYLYRERDPHNEGLVYIRHNWESGQDNSPLWDSVWQRLTLRPDQIPPYQRADTTHV-DAADRPTNAEYDRYVYLVKLFADHGYDEAQIRADCPFLIQDVLFNTLLCQSNRDLAEIARILG--EDALPFEAWAERTSQAINRKLWNEQHGIYVNYDLVLGEQIPVHVAAGFTPLWANIPNVTQAQRMYQYLNSPAFCALDEPCYPVPSYNRQAPGYSPNRYWRGPVWINVDWLLLHGLRRYG-----FDEYAMHLQQSMINLVRHHGFYEYFNPETGQGHGTDQFS 422          

BLAST of Gcaud3165.t1 vs. uniprot
Match: A0A510HIC2_9ACTN (Uncharacterized protein n=2 Tax=Rubrobacter xylanophilus TaxID=49319 RepID=A0A510HIC2_9ACTN)

HSP 1 Score: 379 bits (973), Expect = 2.600e-123
Identity = 201/454 (44.27%), Postives = 265/454 (58.37%), Query Frame = 0

Query:   31 QHQFITDAKAVLDGNWVPTMNSTLPSPNLYPHQWSWDSSFISIGYSHYDTQKAIDETDALFRAQWRNGLLPHIVFNPNADESYFPGPSFWKIETSNSYPLNSHTSGIVQPPVHAIASLAIFNNARSAEQKKLALEHLSTIYPKLVNWHNYLYGERDPLNENLVYIRHMWESGMDNSPAWDDALNSIQLTADQIPQYTRVDKGKVGDHHERPSAHFYDRAVHLIKIFYDNQYDESAIFAKSPFIIQDVLFNSILAHAGDALADIAHILGKNEEEERHRIQAARTAEAISSKLYHEEDGFFYDYDMKSEKLIRTKISGGLVGVYGAKMDSAKLKSILGHLREPGFLGPDLSGFTIPSVSRDDPGYTNTTYWKGPAWININYLVRDGLLRNANGNQDAEEIASYLKERSMDMLKTSGFYEYFNPISGSPHGGHQFSWSAALTIDWMCNASTSSDEVR 484
            +   +  AK VLD NW  T   T P P LYPHQWSWDS+ ISIGYSHYD  +A  E   LF AQWRNGLLP IVFNP+  + YFPGP FW  + S   P N  TSG+VQPPVHA A   ++  AR   +   A E L   YP LV WH+YL+ ERDP +E L YIRH WESGMDNSP WD  L  + L  D+IP Y RVD   V +  +RP++  YDR  +L+++F  + YDE+AI  + PF++QDVLFNS+L  A   LA++A  +G  E+   HR +A RTA AI  KL+ EE G + DYD+ + + I    +   + ++    D  + + ++  L   GF   D     +PS      G++ T YW+GP WINI++ +  GL R       A      L     ++ + SGFYEYF+P +G  HG   FSWSAAL +D +  A+  +   R
Sbjct:    5 ERALLEQAKLVLDFNW--TGGYTKPGPRLYPHQWSWDSALISIGYSHYDQDRAESELLHLFDAQWRNGLLPQIVFNPSFGD-YFPGPGFWHAQESPDAPRNHRTSGVVQPPVHATAVRLVYERARDRGR---AREFLERAYPHLVFWHDYLHRERDPRDEGLAYIRHPWESGMDNSPLWDAILQRLHLREDEIPSYRRVDVHTVSEQ-DRPTSAAYDRFAYLVRLFATHGYDEAAIREECPFLVQDVLFNSLLCRAEADLAELAPEIG--EDPAPHRERARRTARAIDEKLWDEEHGTYLDYDLAAGRPIHVYCASNFLPLFAGVPDEERARRMVDFLEHGGFGLSDRRMRPVPSYDVHGFGFSPTRYWRGPVWINIDWFLLRGLERYGYREHSAR-----LSTTIRELCERSGFYEYFDPFTGQGHGSVLFSWSAALLVDVLLAAADEAPSPR 444          

BLAST of Gcaud3165.t1 vs. uniprot
Match: Q1ATH4_RUBXD (Uncharacterized protein n=2 Tax=Rubrobacter xylanophilus TaxID=49319 RepID=Q1ATH4_RUBXD)

HSP 1 Score: 378 bits (971), Expect = 4.410e-123
Identity = 201/451 (44.57%), Postives = 269/451 (59.65%), Query Frame = 0

Query:   29 PTQHQFITDAKAVLDGNWVPTMNSTLPSPNLYPHQWSWDSSFISIGYSHYDTQKAIDETDALFRAQWRNGLLPHIVFNPNADESYFPGPSFWKIETSNSYPLNSHTSGIVQPPVHAIASLAIFNNARSAEQKKLALEHLSTIYPKLVNWHNYLYGERDPLNENLVYIRHMWESGMDNSPAWDDALNSIQLTADQIPQYTRVDKGKVGDHHERPSAHFYDRAVHLIKIFYDNQYDESAIFAKSPFIIQDVLFNSILAHAGDALADIAHILGKNEEEERHRIQAARTAEAISSKLYHEEDGFFYDYDMKSEKLIRTKISGGLVGVYGAKMDSAKLKSILGHLREPGFLGPDLSGFTIPSVSRDDP---GYTNTTYWKGPAWININYLVRDGLLRNANGNQDAEEIASYLKERSMDMLKTSGFYEYFNPISGSPHGGHQFSWSAALTIDWMCNA 476
            P +   I  AK VL+ NW  T   T P P LYPHQWSWDS+ ISIGYSHYD  +A  E   L  A+WRNGLLP IVFNP+   +YFPGP FW  + S   P    TSG+VQPPVHA A+  ++  A     ++ A   L   YP L  WH+YLY ERDP  E L YIRH WESGMDN+P WD  L  + L  D+IP Y RVD   V +  +RP++  YDR  +L+K+F +  YDE+ I  + PF++QDVLFNS+L  AG+ LA++A  +G  E+   HR +A RTA A+  KL+ EE G + DYD+ +++ I    +   + ++    D+ K + ++  L   GF    LSG  I  V   D    G++ T YW+GP WIN+++ +  GL R   G Q     A  L++  +++ + SGFYEYF+P +G  HG   FSWSAAL ID +  A
Sbjct:    3 PRERALIEQAKLVLEFNW--TGGYTKPGPRLYPHQWSWDSALISIGYSHYDQGRAESELLHLLDARWRNGLLPQIVFNPSFG-NYFPGPGFWHAQESPDAPEGHKTSGVVQPPVHATAARLVYERA---PDRRRARGFLERAYPHLAAWHDYLYRERDPEGEGLAYIRHPWESGMDNAPLWDAILQRMHLREDEIPAYRRVDVHTVSEQ-DRPTSAAYDRFAYLVKLFAELGYDEAGIRRRCPFLVQDVLFNSLLCRAGEDLAELASEVG--EDPSPHRERARRTARALDGKLWDEEHGTYLDYDLVAKRPIHVYCAANFLPLFAGVPDAGKARRMVDFLERGGF---GLSGGHIKPVPSYDVRGFGFSPTQYWRGPVWINMDWFLMRGLRRYGYGEQ-----AGRLRDAIVELCEKSGFYEYFDPATGQGHGSVLFSWSAALLIDALLGA 436          

BLAST of Gcaud3165.t1 vs. uniprot
Match: A0A4R1BL03_9ACTN (Glycoside hydrolase n=1 Tax=Rubrobacter taiwanensis TaxID=185139 RepID=A0A4R1BL03_9ACTN)

HSP 1 Score: 374 bits (959), Expect = 2.240e-121
Identity = 197/437 (45.08%), Postives = 264/437 (60.41%), Query Frame = 0

Query:   35 ITDAKAVLDGNWVPTMNSTLPSPNLYPHQWSWDSSFISIGYSHYDTQKAIDETDALFRAQWRNGLLPHIVFNPNADESYFPGPSFWKIETSNSYPLNSHTSGIVQPPVHAIASLAIFNNARSAEQKKLALEHLSTIYPKLVNWHNYLYGERDPLNENLVYIRHMWESGMDNSPAWDDALNSIQLTADQIPQYTRVDKGKVGDHHERPSAHFYDRAVHLIKIFYDNQYDESAIFAKSPFIIQDVLFNSILAHAGDALADIAHILGKNEEEERHRIQAARTAEAISSKLYHEEDGFFYDYDMKSEKLIRTKISGGLVGVYGAKMDSAKLKSILGHLREPGFLGPDLSGFTIPSVSRDDPGYTNTTYWKGPAWININYLVRDGLLRNANGNQDAEEIASYLKERSMDMLKTSGFYEYFNPISGSPHGGHQFSWSAALTID 471
            I  AK VL+ NW  T   T P P LYPHQWSWDS+ ISIGYS Y  ++A  E   LFR+QW+NGLLPH+VF+P   E YFPG  FW    S   P +  TSG+VQPPVHA A+L ++   R  E    A   L+ ++PKL  WH+YLY ERDP  E LVYIRH WESGMDNSP WD  L  +QL+ ++IP Y R D   V D  +RP    YDR  +LI++F    YDE+ I   SPF++Q+VLFN++L  A   LA++A  LG  E+  R  I+AA+TA A++ KL+ E+ G + DYD++S  LIR   + G + ++    D  + +S+L  +   GF      G   PS      G++   YW+GP WININ+ +  G  R     Q     A +L+  ++ +++  GFYEYFNP++G  HG   FSW+AAL +D
Sbjct:    9 IRQAKMVLEFNW--TGEYTRPGPRLYPHQWSWDSALISIGYSRYAPERAAQELLHLFRSQWKNGLLPHVVFDPGFSE-YFPGIGFWDAHLSPDSPDDHQTSGVVQPPVHATAALELYR--RLGED---ARPFLTELFPKLAAWHDYLYRERDPEGEGLVYIRHPWESGMDNSPIWDPILLGMQLSPEEIPAYQRADTHIV-DPEDRPEMSAYDRYAYLIRLFAGYGYDEAEIRNSSPFLVQNVLFNALLCRAERDLAELARALG--EDPTRFEIRAAKTARAMNGKLWDEKHGTYLDYDLRSGTLIRVYAASGYLPLFAGIPDGNQAQSMLRSMEHHGFYPAKSGGICAPSYDMYGYGFSPVQYWRGPVWININWFLLRGFERYGFTEQ-----ADHLRATTIRLVRDRGFYEYFNPLTGRGHGSDFFSWTAALLLD 429          

BLAST of Gcaud3165.t1 vs. uniprot
Match: A0A838JPD5_9ACTN (Glycoside hydrolase n=1 Tax=Rubrobacteraceae bacterium TaxID=2740537 RepID=A0A838JPD5_9ACTN)

HSP 1 Score: 372 bits (956), Expect = 6.740e-121
Identity = 195/441 (44.22%), Postives = 259/441 (58.73%), Query Frame = 0

Query:   35 ITDAKAVLDGNWVPTMNSTLPSPNLYPHQWSWDSSFISIGYSHYDTQKAIDETDALFRAQWRNGLLPHIVFNPNADESYFPGPSFWKIETSNSYPLNSHTSGIVQPPVHAIASLAIFNNARSAEQKKLALEHLSTIYPKLVNWHNYLYGERDPLNENLVYIRHMWESGMDNSPAWDDALNSIQLTADQIPQYTRVDKGKVGDHHERPSAHFYDRAVHLIKIFYDNQYDESAIFAKSPFIIQDVLFNSILAHAGDALADIAHILGKNEEEERHRIQAARTAEAISSKLYHEEDGFFYDYDMKSEKLIRTKISGGLVGVYGAKMDSAKLKSILGHLREPGFLGPDLSGFTIPSVSRDDPGYTNTTYWKGPAWININYLVRDGLLRNANGNQDAEEIASYLKERSMDMLKTSGFYEYFNPISGSPHGGHQFSWSAALTIDWMCN 475
            +  AKA+L  NW  T   T P P LYPHQWSWDS+ I+I Y+HYD ++A  E   LF AQW NGLLP IVFNP   + YFPGPSFW  + S   P +  TSG+V PPVHA A LAI+  A   E+ K   E L   + KL  WH YLY +RDP  E LVYIRH WESGMDNSP WD  +  + L A QIP+Y R D   V    +RP++  YDR  +L+K F D  YDE  I    PF++QDVLFNS+L  +G  +A IA +LG  E+ +    QA +TA AI  KL+ EE G ++DYD+ +++ I    +     ++    + A+ K ++  L   GF   D +   +PS+     G++   YW+GP W+NIN+ +  GL      +    E A  L+   + + + +GF+EYFNP +G  HG   FSWSAAL ID + N
Sbjct:    9 VEQAKAILALNW--TGEYTQPGPRLYPHQWSWDSALIAIAYAHYDRKRAEKELRHLFEAQWTNGLLPQIVFNPRFRD-YFPGPSFWNADESPHAPKHKETSGVVLPPVHATAVLAIYKAA--GEEAK---EFLGYAFRKLKAWHEYLYRDRDPRGEGLVYIRHPWESGMDNSPTWDQIMQRMHLRAGQIPKYKRADTNTVSAQ-DRPTSASYDRFAYLVKFFADRNYDEGRIREDCPFLVQDVLFNSLLCRSGHDMAGIARVLG--EDPDPFEEQAQKTARAIEDKLWSEEHGVYFDYDLVADRPIHVYFAPNFAPLFAGTPNEARAKRLVDALEHDGFGLSDKNVTPVPSLDMHGFGFSPIEYWRGPVWLNINWFLMHGL-----EDYGYREYADRLRAGIIGLCREAGFHEYFNPTTGMGHGSDLFSWSAALLIDVLMN 433