Gcaud2574.t1 (polypeptide) Gracilaria caudata M_176_S67 male

BLAST of Gcaud2574.t1 vs. uniprot
Match: A0A2V3IVY5_9FLOR (DNA ligase IV n=1 Tax=Gracilariopsis chorda TaxID=448386 RepID=A0A2V3IVY5_9FLOR)

HSP 1 Score: 1267 bits (3279), Expect = 0.000e+0
Identity = 636/1044 (60.92%), Postives = 810/1044 (77.59%), Query Frame = 0

Query:   21 VDDVPFSILSELYQQLRKARTAQSTSHFHSARQAHSRRIAILTQVWTRIANTCNHS---PNHQPTHHAQNIIPAHLFPPAESFKLVSLIVPTLDSQHAYTGMRESRLADTFIKALDLNVNGEDAQWLKHYKEKEYRSSRCKYDADIVDGNLPTVIEAVLKGRCPSKSSLTVGMVWNMLHTLSQTARKKSRRIGKVFYASAEQDNFAAPRLRAQTEDLSDPEDMRYDAIRKIVIHGTREEVAEVSRIILKDLDIRVREDSFLSWFHPAAKQHYTQMHDIQKLLQDCHDPKFEIGAPSVLVGSYASVMLTMRPSKRQLDVVCKNLRGNRGITASSSDAIDDSYFMMEPKLDGERMQLHKWTIESEQGRGLQTQIRTFSRRGNDSSAMYAEALNEVVLTAVRAKDIILDGEIMIWNKNAEAWLRFEDMREITTSISKKKVPEGSSYILKFMVFDVLYIVQEDGDRAQKAANMVMKLPLYQRRQLLKRIIQPRKASFCPGVLTEIELVEMEKGHNERELIQALQRYETLGYEGVIAKHPDRPYVLAERNLNIAIKLKPDYFDGGIQDLDCLILGARYSDSHGHRVQRAGRLSSFLIGVRATDLTSTSSSKRDEEWAERMKKTKWIPVGNIGTGYADSELAELQNKFEHDWKNFNLHDLPEHFERREYTSTLLSGVAKWIQPWNSVVVTVTAYELNRRVWALRFPRVQRINWEKPYYDVPTFDHLKDLDDNKKPQTIVSDENDVDEVRETKSKRKRKNVLGSDEEDAVKRVKEEGHIVTGGRNPRTLISSAAGVDVSNLERKSTAFEGVCCHVITENPEQKENLEIKIHELGGTFSQTVSSGVDFVICTRATLQRVQMLKDLLSSKDGSNRTFSILRSTWVDESHSRLRKMVIDRSHVVHATEKLAADVYKVVDRFGDHWDRDADEHSIERALDEAKRWKTE-NPGYKDD-VGIKEVEERVSKVVEECKNIFSNINVFVPHTQFDVSGTKILLEAFGARVVNEVVSDCTHVLVHSSLRDKHQKIATA-EETVITELWVEKCLEAGKILG 1058
            VD++PFSIL+ LY+ LR AR  +  S  ++ +QA  RR  +L+  WT IA  C  +   P+ Q  H  +N +PAH+FPP+ESFKLVSL+VP LDS HAY GM+ES+LAD FIK+LDL+ NG DAQWL+HYKEK+YR  + K D DIVDGNLPTV++A+LK RC  +SSLT+G VWN L TL+QTA+++ RRI KVF    E  + +   +  +  D   P+ +R +AIR +V  GT E+V+EVSRI+LKDLDIR+ EDSFL+WFHPAAKQHYTQMHDI KLL DCHDPKFEIG   V +G YASVMLTMRPS+R+LD+VCKNL GN     SSS+  D+SYF+MEPKLDGERMQLHKW  +S+        IRTFSRRGNDSSAMYAEAL E VL AV+A+D+ILDGEIMIW++   +W+RFEDMRE++TSI+K+ V E +SY+L+FMVFDVLYI Q+D + A++AA+ V++LPLYQRR+LLK+IIQP++ SF  GV T +EL+ ME+G NE ELIQALQRYETLGYEGVIAK+PD+PY+LAERN+ +AIKLKPDYFDGGIQDLDCLILGARYSDS GHR QRAG LSSFLIGVR+T+L ++S    +EEW ERMKK KWIPVG+IGTGY+D ELA+LQ++F  +WK+F+  DLPEHFE+REY  +LL+GVAKWIQPW SVVVTV AYELNRR+ ALRFPRVQRINWEKPYYDVPTF  L DLDDNK P  +V+DENDVD+V +  +K++++  L SDEEDAV+R + EGHIVTGGR+ R LISSAAG+DVSN++RK++AF+  C +V+ E+ + KE LE+KIHELGG F Q+++S +DFVICT  +L + + LK ++SS     +  SILR  WVD+ H RL ++ I RS VV A++ LAA V+K  DRFGD+WDR+ DE SI  +L E ++W  E N   KD+ +GI E    VS+V   C N+F+N NVFVP  +  ++G+K+LLEA+GA++V ++      VL+HSSLRD+ +K   A +  +ITE+WVE+C++AGK+L 
Sbjct:   16 VDEIPFSILTNLYRDLRHARATRQRSASNNGKQADLRRGTLLSAAWTHIAQLCGRTSQLPSIQ--HDNRNFVPAHVFPPSESFKLVSLLVPALDSAHAYMGMKESKLADCFIKSLDLDPNGADAQWLRHYKEKDYRPQKWKRDIDIVDGNLPTVLKALLKERCAQESSLTIGCVWNALETLTQTAKRRHRRIAKVFQLP-EPSSTSNASVAGRQHDSFQPDVLRANAIRSLVSSGTAEDVSEVSRIVLKDLDIRLNEDSFLNWFHPAAKQHYTQMHDIHKLLSDCHDPKFEIGEACVQIGHYASVMLTMRPSRRKLDLVCKNLLGNGPAEPSSSENADNSYFIMEPKLDGERMQLHKWKAKSQSEDFGGYNIRTFSRRGNDSSAMYAEALTEAVLLAVKAEDVILDGEIMIWDELRASWVRFEDMREVSTSIAKRSVSESASYVLRFMVFDVLYIAQDDSEDARRAASKVIRLPLYQRRELLKKIIQPKEVSFGSGVRTRVELIRMERGSNETELIQALQRYETLGYEGVIAKNPDQPYLLAERNIRVAIKLKPDYFDGGIQDLDCLILGARYSDSRGHRTQRAGSLSSFLIGVRSTELYASSQMHGNEEWEERMKKCKWIPVGSIGTGYSDDELAQLQHRFRGEWKDFHNLDLPEHFEQREYAPSLLAGVAKWIQPWRSVVVTVQAYELNRRLSALRFPRVQRINWEKPYYDVPTFTQLIDLDDNKTPPVVVADENDVDDVLDIGNKKRKRGTLESDEEDAVQRARNEGHIVTGGRSTRKLISSAAGLDVSNVKRKTSAFQNTCFYVVAEDCKSKEELELKIHELGGRFLQSLTSEIDFVICTTPSLPKARRLKHVISSNKNDTQKCSILRRVWVDDCHDRLSRIDIRRSDVVLASQTLAAIVFKFADRFGDYWDRNCDEVSIAESLREVEKWTKETNIDDKDEPLGINEGLPEVSRV---CNNVFANTNVFVPPLRIGLAGSKLLLEAYGAQLVEKLDERTEFVLIHSSLRDELEKDNPAIQSKIITEIWVEECIDAGKLLS 1053          

BLAST of Gcaud2574.t1 vs. uniprot
Match: R7Q5C6_CHOCR (DNA ligase IV n=1 Tax=Chondrus crispus TaxID=2769 RepID=R7Q5C6_CHOCR)

HSP 1 Score: 1092 bits (2825), Expect = 0.000e+0
Identity = 570/1065 (53.52%), Postives = 749/1065 (70.33%), Query Frame = 0

Query:    9 PNQKQQPSSHIIVDDVPFSILSELYQQLRKARTA--QSTSHFHSARQAHSRRIAILTQVWTRIANTCNHSPNHQPTHHAQN-IIPAHLFPPAESFKLVSLIVPTLDSQHAYTGMRESRLADTFIKALDLNVNGEDAQWLKHYKEKEYRSSRCKYDADIVDGNLPTVIEAVLKGRCPSKSSLTVGMVWNMLHTLSQTARKKSRRIGKVFYASAEQDNFAAPRLRAQTED--LSDPEDMRYDAIRKIVIHGTREEVAEVSRIILKDLDIRVREDSFLSWFHPAAKQHYTQMHDIQKLLQDCHDPKFEIGAPSVLVGSYASVMLTMRPSKRQLDVVCKNLRG----------NRGITASSSDAIDDS--YFMMEPKLDGERMQLHKWTIESEQGRGLQTQIRTFSRRGNDSSAMYAEALNEVVLTAVRAKDIILDGEIMIWNKNAEAWLRFEDMREITTSISKKKVPEGSSYILKFMVFDVLYIVQ--EDGDRAQKAANMVMKLPLYQRRQLLKRIIQPRKASFCPGVLTEIELVEMEKGHNERELIQALQRYETLGYEGVIAKHPDRPYVLAERNLNIAIKLKPDYFDGGIQDLDCLILGARYSDSHGHRVQRAGRLSSFLIGVRATDLTSTSSSKRDEEWAERMKKTKWIPVGNIGTGYADSELAELQNKFEHDWKNFNLHDLPEHFERREYTSTLLSGVAKWIQPWNSVVVTVTAYELNRRVWALRFPRVQRINWEKPYYDVPTFDHLKDLDDNKKPQTIVSDENDVDEVRETKSKRKRKNVLGSDEEDAVKRVKEEGHIVTGGRNPRTLISSAAGVDVSNLERKSTAFEGVCCHVITENPEQKENLEIKIHELGGTFSQTVSSGVDFVICTRATLQRVQMLKDLLSSKDGSNRTFSILRSTWVDESHSRLRKMVIDRSHVVHATEKLAADVYKVVDRFGDHWDRDADEHSIERALDEAKRWKTENP--GYKDDVGIKEVEERVSKVVEECKNIFSNINVFVPHTQFDVSGTKILLEAFGARVVNE--VVSDCTHVLVHSSLRDKHQKI-ATAEETVITELWVEK 1049
            P   + P     V+ +PFS+++ L++ LRKA+    + T   H       ++ AILT  W +I   C    +     +A +  IPA L PPAESFK++SL+VP LDS+HAY GM+E++LAD F+KALDL  NG+DAQWLKH+KEKEYR  R K DADIVDGN  TV+ AVL  RCP +S LTVG+VW  L T+S+ +R +SRRI + F    E  N      R   +D  +S PED +  A+R +V  GT +EVAEV+RIILKD+DIR+ ED FLSWFHP AKQHYTQ+HDI +LL+DC DP FEIG  SV VG YASVML MRPS+++L+V+C+NLRG              T ++SD   ++  YF+MEPKLDGER+QLHK +  S+ G G+Q ++RTFSRRGN+SSAMYA A+ EV+L  V+A++IILDGEIMIW+    +WLRFED RE+ TSI+K+ VPEGSSY LK+MVFDVLY+ Q       A+++ NMV++LPL++RR LL++++  R+AS+  G    IE+V+ME G+NER+L QALQRYETLGYEGVIAK+PD PY LAER+L++AIKLKPDYFDGGIQDLD LILGA+YS S GHR QRAGRLSSFLIGVRA D T      R  EW +RM+K KW+PVG++GTGY+DSEL ++QN    +WK FN  DLPEHFE R+Y  +LLS V KWI+PW S+V+TV A+E+NRR +ALRFPRV+RINW+KPY+DVP+F HL DLD+NK P TI  DE+D+D++ E    +K + +  ++EE+AVKRVK EGH++TGGRNPR++I+SA G DV+ ++R STAFEG+   VI  NP+ KE +E++IH LGG F Q   S VDFVICT   + RV++LK+  S+      T SI+ S WV+E  S   + +   + VV+A   L +++++  DRFGD W  +     +  +LDE  +W  + P  G  DDV ++ V ERV + ++ C N+F  + V VP T+ +V G+  LL AFG RVV+E  V  DCTHVLVHSSL    QK+  + +  VITE WV +
Sbjct:   10 PQNSKAPRDECNVNALPFSVITTLFRDLRKAKVPAKRRTGGLHVKSDRFGKK-AILTNAWLKIGEKCGVGEDVLARRNANDHFIPAQLLPPAESFKILSLLVPGLDSEHAYLGMKETKLADAFVKALDLLPNGDDAQWLKHHKEKEYRPQRWKQDADIVDGNFATVLRAVLDERCPKESLLTVGIVWRALDTMSKASRVRSRRIQRAFPRQNEMMNETTMTDRTTDDDESMSKPEDGKVVALRTLVQCGTSDEVAEVARIILKDVDIRLSEDMFLSWFHPGAKQHYTQIHDIHQLLKDCSDPHFEIGDASVQVGQYASVMLCMRPSRKKLNVICENLRGAGEPAEPETQKELETRTTSDLSQEARQYFIMEPKLDGERLQLHKRSTHSDDGTGVQ-EVRTFSRRGNESSAMYAGAMREVILAGVQAENIILDGEIMIWDNLRSSWLRFEDTREVATSIAKRNVPEGSSYTLKYMVFDVLYVDQGQRKHSEARRSGNMVIRLPLHKRRILLEKLVTKREASYGIGATACIEVVDMELGYNERDLTQALQRYETLGYEGVIAKNPDMPYALAERSLDLAIKLKPDYFDGGIQDLDVLILGAKYSSSRGHRKQRAGRLSSFLIGVRANDGTPPWKG-RGGEWEQRMQKCKWVPVGSVGTGYSDSELEQMQNMLSGEWKTFNAKDLPEHFEGRDYAPSLLSDVVKWIEPWKSIVLTVRAFEVNRRYFALRFPRVERINWDKPYFDVPSFSHLLDLDENKLPATIRPDESDIDDISEYTGGKKPRRLFDAEEEEAVKRVKLEGHVITGGRNPRSVIASAVGADVTQVDRVSTAFEGLTFFVIASNPKLKEEVELQIHRLGGKFVQNFGSSVDFVICTSIHVSRVKILKERFSNIREKEDTCSIVHSNWVEECLSTKTRTLPSLNDVVYANRTLESELFEKADRFGDSWKEETSITELVHSLDEVSKWTAKQPEVGNGDDV-LEGVRERVKRAMKRCGNVFHGLTVLVPDTEVEVHGSVALLSAFGGRVVSEKEVDGDCTHVLVHSSLVPTWQKLHGSTKGAVITEEWVRE 1070          

BLAST of Gcaud2574.t1 vs. uniprot
Match: M2WXC4_GALSU (DNA ligase 4 n=1 Tax=Galdieria sulphuraria TaxID=130081 RepID=M2WXC4_GALSU)

HSP 1 Score: 356 bits (913), Expect = 1.320e-103
Identity = 259/874 (29.63%), Postives = 427/874 (48.86%), Query Frame = 0

Query:   89 IPAHLFPPAESFKLVSLIVPTLDSQHAYTGMRESRLADTFIKALDLNVNGEDAQWLKHYKEKEYRSSRCKYDADIVDGNLPTVIEAVLKGRCPSKSSLTVGMVWNMLHTLSQTARKKSRRIGKVFYASAEQDNFAAPRLRAQTEDLSDPEDMRYDAIRKIVIHGTREEVAEVSRIILKDLDIRVREDSFLSWFHPAAKQHYTQMHDIQKLLQDCHDPKFEIGAPSVLVGSYASVMLTMRPSKRQLDVVCKNLRGNRGITASSSDAIDDSYFMMEPKLDGERMQLHKWTIESEQGRGLQTQIRTFSRRGNDSSAMYAEALNEVVLTAVRAKDIILDGEIMIWNKNAEAWLRFEDMREITTSISKKKVPEGSSYILKFMVFDVLYIVQEDGDRAQKAANMVMKLPLYQRRQLLKRIIQPRKASFCPGVLTEIELVEMEKGHNERELIQALQRYETLGYEGVIAKHPDRPYVLAERNLNIAIKLKPDYFDGGIQDLDCLILGARYSDSHGHRVQRAGRLSSFLIGVRATDLTSTSSSKRDEEWAERMKKTKWIPVGNIGTGYADSELAELQNKFEHDWKNFNLHDLPEHFERREYTSTLLSGVAK-WIQPWNSVVVTVTAYELNRRV----WALRFPRVQRINWEKPYYDVPTFDHLKDLDDNKKPQTIVSDENDVDEVRETKSKRKRKNVLGSDEEDAVKRVKEEGHIVTGGRNPRTLISSAAGVDVSNLERKSTAFEG-VCCHVITENPEQKENLEIKIHELGGTFSQTVSSGVDFVIC-----------TRATLQRVQMLKDLLSSKDGSNRTFSILRSTWVDESHSRLRKMVIDRSHVVHATEKLAADVYKVVDRFGDHWDRDADEHSIERAL 945
            IPA    P +SF ++ L++P LD+     GM+E  LA+ +I   +L+ +  DAQ L+ +++   +    +  A  + GN  +V+EAVL+ RC   SSLT+  V  +L+ L+ T  K+ R+  K+F+                                +IV   T  E   +SRIILK + + +R +S L WFHP+A + Y   H ++++ +DC  P F I +  V++G  ASV L    +KRQ     +NL+    I    SD      F +EPK DGER+QLHK   +          I+ FSR   D++ +Y   L   VL  V A+D +LDGE++++N + +A+  F+ +R   +     K+ +   +++ +M+FDVLY+     D+ Q     V+  PL  R+++LK I++P  +        +I LV  ++G    ++ QA++ +    YEGV+ K+  +PY+L +R+  + IKLKPDYF+  + DLD LILGA     +  +  R G+ S FLIG+   D+          E  +R  +  WI +G +G+GY+  +L +LQ+K    WK ++    PEH +   ++     GV   WI+P +S+V+T+ AYE+        +A+RFPRV  I W+K +++  T D L       +P    S    VD ++  K +R            A   ++E+  +++    P                + S   +G V C V   + ++K+  E  +H+LGG   Q+V+    FV+             R+    +Q +K  L S+ G     SIL+  W+ E   + R + I   HVV AT+ L  ++    D +GD W +  +E  +   L
Sbjct:   45 IPARSVSPLDSFSILRLLLPELDNDRRSFGMKERGLAELYISVFELDPSSFDAQRLRRWQDPTVQQQTGQIPA--IPGNFFSVLEAVLRHRCSDTSSLTISQVNELLNELNITLEKEKRK--KIFH--------------------------------QIVTECTVTENIWISRIILKQMHLGLRFESILKWFHPSALEDYRLRHSLRRMCEDCFQPGFYIVSQDVILGQPASVSL----AKRQ-----ENLQR---IPYLMSDG-----FFLEPKFDGERLQLHKKGED----------IQCFSRNALDTTHLYGPHLKTAVLLCVDAQDCVLDGEVLLYNVDKQAFEHFDQIRHYISIAKDTKLDDN--FLITYMIFDVLYV-----DQNQNVPPSVVSFPLETRKEILKGIVRPFHS--------QIRLVPYKEGTTVEDIKQAMEDFIEQKYEGVVVKNKKKPYLLGKRDDEVMIKLKPDYFNDLVHDLDVLILGAFIGTGYS-KTTRIGKPSHFLIGILEKDI----------EEQDRNVEVSWIVIGKVGSGYSYEQLEQLQHKLAPFWKPYDKKAPPEHLK---HSLLRADGVPDFWIEPCHSIVLTIYAYEVRPSAIYSGYAVRFPRVANIRWDKRWFECSTLDELT------RPSEPPSASYSVDGLKTNKKRR------------AQPHLREKVDLISVPTRPEAFAL-----------KSSLCLDGLVLCVVGASSNQEKQYYEKLVHQLGGKTVQSVTQDTRFVLAISPNHVAVTNMVRSFQNPLQAVKSKLRSR-GCLEPKSILKVNWLLECQVKDRMVEILPCHVVWATDSLRKEIETRCDCWGDSWHKAVNEEELRELL 796          

BLAST of Gcaud2574.t1 vs. uniprot
Match: G4YG23_PHYSP (DNA ligase n=3 Tax=Phytophthora TaxID=4783 RepID=G4YG23_PHYSP)

HSP 1 Score: 219 bits (557), Expect = 3.940e-55
Identity = 252/1026 (24.56%), Postives = 443/1026 (43.18%), Query Frame = 0

Query:  100 FKLVSLIVPTLDSQHAYTGMRESRLADTFIKALDLNVNGEDAQWLKHYKEKEYRSSRCKYDADIVD----GNLPTVIEAVLKGRCPSKSSLTVGMVWNMLHTLSQTARKKSRRIGKVFYASAEQDNFAAPRLRAQTEDLSDPEDMRYDAIRKIVIHGTREEVAEVSRIILKDLDIRVREDSFLSWFHPAAKQHYTQMHDIQKLLQDCHDPKFEIGAPSVLVGSYASVMLTMRPSKRQLDVVCKNLRGNRGITASSSDAIDDSYFMMEPKLDGERMQLHKWTIESEQGRGLQTQIRTFSRRGNDSSAMYAEALNEVVLTAVR-AKDIILDGEIMIWNKNAEAWLRFEDMREITTSISKKKVPEGSSYILKFMVFDVLYI--------VQEDGDRAQKAANMVMKLPLYQRRQLLKRIIQPRKASFCPGVLTEIELVEMEKGHNER--ELIQALQRYETLGYEGVIAKHPDRPYVLAE--RNLNIAIKLKPDYFDGGIQDLDCLILGARYSDSHGHRVQRAGRLSSFLIGVRATDLTSTSSSKRDEEWAERMKKTKWIPVGNIGTGYADSELAELQNKFEHDWKNFNLHDLPEHFER-REYTSTLLSGVAKWIQPWNSVVVTVTAYELNRRVW-----ALRFPRVQRINWEKPYYDVPTFDHLKDLDDNKKPQTIVSDENDVDEVRETKSKRKRKNVLGSDEEDAVKRVKEEGHIVTGGRNPRTLISSAAGVDVSNLERKSTAFEG--------------------VCCHVITENPEQ--KENLEIKIHELGGTFSQTVSSGVDFVICTRATLQRVQMLKDLLSSKDGSNRTFSILRSTWVDESHSRLRKMVIDRSHVVHATEKLAADVYKVVDRFGDHWDRDADEHSIERALDE-AKRWKTEN---PGYKD----DVGIKEVEERVSKVVEECKNIFSNINVFVPHTQFDVSG-------TKIL---LEAFGARVVNEVVSDCTHVLV---------HSSLRD-KHQKIATAEETVITELWVEKCLE 1052
            + LV L++P LD +  Y  ++E ++A  +++ L L+   +DAQ L+H+           +D  IVD    G+   V+  V+  R  +  S          HTL          +  +  +  + DN A                 R   I K+    + +E   + R++LKDL I +R +  L + HP A + Y   +D+ K+ ++             L  S    +  + P +    ++ K +     I+A ++DA     F+MEPKLDGER+  H       QG+    +++  SR G + +  YA ++   VL+ +    D ILDGE+M+W+        F  ++    ++ K    E ++  L ++V+DV+Y+        +  +  +     + VM LPL+ RR+LL RI++P        ++ E +LV  +K   ER  +++  +    + G EGVI K  +  Y+  E  R     +KLKPDY  G   DLD +I+G  Y    G   +R+G +S FL+GV A  L   S++K  E  A             +GTGY   EL +++ + E  W+ ++  ++P H    +   S L   V  WI P +S ++ V  +EL+          +RFPR + I  +K +Y      +L+DL  N    ++ +               KR + +   ++ A  RVK+   I    R P  ++   +  D+  +E++   FEG                    +   ++ +N +Q  K+ +E  +H  GGT  Q      D+V+       +V  LK     K G    F+I+   W+    +  R + +     V+AT+     + K  D +GDH+        + R + E +  + +EN    G +      V +K++E   ++ ++      ++  V+  H   D          TK+L   L  +G  V  E+ S  THV+V           ++R+ +HQ +   E   +T+ W+E+C+E
Sbjct:   49 YPLVRLVLPQLDRERTYM-LKEKKIAKIYVQVLGLSPASKDAQKLEHF-----------HDPKIVDSKSVGDFAAVLYEVMLYRSTASKS-------RQQHTLKD--------VNALLDSLVQADNNAT----------------RKKVIMKLATEYSADEQKWIIRVVLKDLKIGLRHERVLKFIHPDAMEMYNHTNDLHKVCRE-------------LRNSAVRYVPQLEPFQVFTPMLAKRVNFGECISAINADA-----FVMEPKLDGERITCHV------QGK----EVQFISRNGINYTENYAPSIKPHVLSQLEPGVDCILDGEMMVWDNVGYRLREFGLLKNTANAVRK---GEATNRWLCYVVWDVVYLGGSPKAEQLIREVFKGPGEISAVMGLPLHARRKLLLRILKPLDHRI---IILEQKLVN-DKSAKERHSKVMAEVDEQVSNGGEGVIMKDLNAHYMCGESSRKTKKWLKLKPDYA-GMTTDLDAIIIGGFY----GVGRRRSGNVSVFLLGVLAHSLDENSAAKATEPGAACPTV---YTFAKVGTGYNLDELEQMRQELEPYWQPWDDDNIPPHLNGWKPQKSDLRPDV--WIDPRHSKILEVYGFELSFTTLYSTGLTIRFPRCKAIRNDKEWYQCI---NLQDL--NAARGSLST---------------KRASEVALGQKTATGRVKKRQTI--SSRRPSGVLQGYSRADLEGIEQERNVFEGKEFCVLPGKYEAPPSDVVPAIPSGIVEDNAKQLSKQTVEKLLHAFGGTIVQNPIESTDYVVAAGDAGFKVVNLK-----KQG---LFNIVHIRWIFTCVAVARLISLKAGDFVYATDSQRELLAKEYDHYGDHFTEHIGPADLRRIIRELSSAYMSENRLAAGERHPQPWQVQMKDLEMEEAEAMDCEYTFLAHCVVYFQHFSNDADADALLLGQTKLLEQQLRLYGGVVAGEIDSSVTHVVVADRILAQQAQPAIRNLRHQGLP--EPRTVTKEWIEQCVE 954          

BLAST of Gcaud2574.t1 vs. uniprot
Match: A0A225WYF4_9STRA (DNA ligase n=1 Tax=Phytophthora megakarya TaxID=4795 RepID=A0A225WYF4_9STRA)

HSP 1 Score: 215 bits (547), Expect = 7.040e-54
Identity = 259/1017 (25.47%), Postives = 435/1017 (42.77%), Query Frame = 0

Query:  100 FKLVSLIVPTLDSQHAYTGMRESRLADTFIKALDLNVNGEDAQWLKHYKEKEYRSSRCKYDADIVDGNLPTVIEAVL--KGRCPSKSSLTVGMVWNMLHTLSQTARKKSRRIGKVFYASAEQDNFAAPRLRAQTEDLSDPEDMRYDAIRKIVIHGTREEVAEVSRIILKDLDIRVREDSFLSWFHPAAKQHYTQMHDIQKLLQDCHDPKFEIGAPSVLVGSYASVMLTMRPSKRQLDVVCKNLRGNRGITASSSDAIDDSYFMMEPKLDGERMQLHKWTIESEQGRGLQTQIRTFSRRGNDSSAMYAEALNEVVLTAVR-AKDIILDGEIMIWNKNAEAWLRFEDMREITTSISKKKVPEGSSYILKFMVFDVLYIVQEDGDRAQKAANMVMK----------LPLYQRRQLLKRIIQPRKASFCPGVLTEIELVEMEKGHNERELIQA-LQRYETLGYEGVIAKHPDRPYVLAE--RNLNIAIKLKPDYFDGGIQDLDCLILGARYSDSHGHRVQRAGRLSSFLIGVRATDLTSTSSSKRDEEWAERMKKTKWIPV----GNIGTGYADSELAELQNKFEHDWKNFNLHDLPEHFER-REYTSTLLSGVAKWIQPWNSVVVTVTAYELN-----RRVWALRFPRVQRINWEKPYYDVPTFDHLKDLDDNKKPQTIVSDENDVDEVRETKSKRKRKNVLGSDEEDAVKRVKEEGHIVTGGRNPRTLISSAAGVDVSNLERKSTAFEG--------------------VCCHVITENPEQ--KENLEIKIHELGGTFSQTVSSGVD-FVICTRATLQRVQMLKDLLSSKDGSNRTFSILRSTWVDESHSRLRKMVIDRSHVVHATEKLAADVYKVVDRFGDHWDRDADEHSIERALDEAKRWKTENPGYKDDVGIKEVEERVSKVVE-----ECKNIFSNINVFVPHTQFDVSGTKILLEAFGARVVNEVVSDCTHVLVHS---------SLRD-KHQKIATAEETVITELWVEKCLE 1052
            + L+ L++P LD +  Y  ++E ++A  +I  L L    +DAQ L+H+ + +   S+   D   V      + + VL    R  SK   T+  V  +L  L Q     +R+  KVF   A +                      Y A          +E   + R++LKDL I +R +  L + HP A + Y   +D+ K+ ++             L  S    +  + P +    ++ K +     I+A ++DA     F+MEPKLDGER+  H    E          ++  SR G + +  YA ++   VL+ +    D ILDGE+M+W+ N E  LR   + + T +  +K   E ++  L ++V+DV+Y+    G +A++  + V K          LPL+ RR+LL RI++P        ++ E ++V  +      E + A +    + G EGVI K  +  Y+  E  R     +KLKPDY  G   DLD +I+G  Y        +R+G +S FL+GV A  L    +       AE MK     PV      +GTGY   EL  ++ + E  W+ ++  ++P H    +   S L   V  WI P +S ++ V  +EL+     +    +RFPR + I  +K +Y         DL D    +  +S            +KR  +  LG  ++ A KR K+   I    R P  +I   +  D+  +E++   FEG                    +   +I +N +Q  K+ +E  +H  GGT  Q   +G   +V+       +V  LK     K G    F+I+   WV    +  R + +   + + AT+     + K  DR+GDH+        + R L E      +   ++  + + ++EE  +   E      C   F + +   P     V   +  L  +G  VV E+ S  TH++V           ++R+ +H+ +   E  V+T+ WV++C E
Sbjct:   58 YPLIRLVLPQLDRERTYM-LKEKKIAKIYIDVLGLAPISKDAQKLEHFNDPKVVDSKSVGDFAAV------LFDVVLYQSARTKSKQQHTLQDVNGLLDQLVQANSNVARK--KVFMTLATE----------------------YSA----------DEQKWIIRVVLKDLKIGLRHERVLKFIHPDAMEMYNHTNDLHKVCKE-------------LRNSAVRYVPQLEPFQVFTPMLAKRVNFGECISAINADA-----FVMEPKLDGERITCHVHGKE----------VQFISRNGINYTENYAPSIKPHVLSQLEPGVDCILDGEMMVWD-NTEYRLREFGLLKNTANAVRK--GEATNRWLCYVVWDVVYL--SGGSKAEQLIHEVFKGPGEISAVMGLPLHARRKLLLRILKPLDHRI---IIIEQKIVNDKSAKERHERVMAEVDEQVSNGGEGVIMKDLNAHYMCGESSRKTKKWLKLKPDYA-GMTTDLDAIIVGGFYGTGR----RRSGNVSVFLLGVLAHSLDENGA-------AEAMKPGAPCPVVYTFAKVGTGYNLEELEHMRLELEPHWQAWDDDNVPPHLNGWKPQKSDLRPDV--WIDPRHSKILEVYGFELSFTTLYQTGLTIRFPRCKAIRNDKEWYQ------CIDLQDLNAARGSLS------------TKRASEVALG--QKSAAKRTKKRQTI--SARRPNGVIKDYSRADLEGIEQERNLFEGKEFCVLPGKYEFPQTTILSLIPGGIIEKNAKQLSKQTIEKLLHSFGGTIVQNPIAGTTHYVVAAGDAGFKVVNLK-----KQG---LFNIVHIRWVFNCVAASRLVSLKAGNFIFATDSQRELLAKEYDRYGDHYTEHITPDDLHRILLELPEDGPKGLPWQIQMQVLDMEEAEALDSEFTFLCHCVVYFQDCSEDDPLYVGHVKLLEQQLMLYGGVVVREIDSTVTHIVVTDPSQVQQFVPAIRNFRHRGLP--EPRVVTKEWVQQCAE 951          

BLAST of Gcaud2574.t1 vs. uniprot
Match: D0MXT4_PHYIT (DNA ligase n=12 Tax=Phytophthora TaxID=4783 RepID=D0MXT4_PHYIT)

HSP 1 Score: 213 bits (543), Expect = 2.330e-53
Identity = 258/1027 (25.12%), Postives = 444/1027 (43.23%), Query Frame = 0

Query:  100 FKLVSLIVPTLDSQHAYTGMRESRLADTFIKALDLNVNGEDAQWLKHYKEKEYRSSRCKYDADIVDGNLPTVIEAVL--KGRCPSKSSLTVGMVWNMLHTLSQTARKKSRRIGKVFYASAEQDNFAAPRLRAQTEDLSDPEDMRYDAIRKIVIHGTREEVAEVSRIILKDLDIRVREDSFLSWFHPAAKQHYTQMHDIQKLLQDCHDPKFEIGAPSVLVGSYASVMLTMRPSKRQLDVVCKNLRGNRGITASSSDAIDDSYFMMEPKLDGERMQLHKWTIESEQGRGLQTQIRTFSRRGNDSSAMYAEALNEVVLTAVRAK-DIILDGEIMIWNKNAEAWLRFEDMREITTSISKKKVPEGSSYILKFMVFDVLYIVQEDGDRAQKAANMVMK----------LPLYQRRQLLKRIIQPRKASFCPGVLTEIELVEMEKGHNERELIQA-LQRYETLGYEGVIAKHPDRPYVLAE--RNLNIAIKLKPDYFDGGIQDLDCLILGARYSDSHGHRVQRAGRLSSFLIGVRATDLTSTSSSKRDEEWAERMKKTKWIPV----GNIGTGYADSELAELQNKFEHDWKNFNLHDLPEHFER-REYTSTLLSGVAKWIQPWNSVVVTVTAYELN-----RRVWALRFPRVQRINWEKPYYDVPTFDHLKDLDDNKKPQTIVSDENDVDEVRETKSKRKRKNVLGSDEEDAVKRVKEEGHIVTGGRNPRTLISSAAGVDVSNLERKSTAFEGVCCHVIT---ENPEQ-------------------KENLEIKIHELGGTFSQT-VSSGVDFVICTRATLQRVQMLKDLLSSKDGSNRTFSILRSTWVDESHSRLRKMVIDRSHVVHATEKLAADVYKVVDRFGDHWDRDADEHSIERALDE----AKRWKTENPGYKDDVGIKEVEERVSKVVEECKNIFSNINVFVPHTQ------FDV--SGTKILLEA----FGARVVNEVVSDCTHVLVHSS--------LRD-KHQKIATAEETVITELWVEKCLE 1052
            + L+ L++P LD +  Y  ++E ++A  +I  L L    +DAQ L+H+ + +   S+   D   V      + + VL    R  SK+  T+  V  +L  L Q     +R+  KVF                                 K+    + +E   + R+ILKDL I +R +  L + HP A + Y   +D+ K+ ++             L  S    +  + P +    ++ K +     I+A ++DA     F+MEPKLDGER+  H       QG+    +++  SR G + +  YA ++   VL+ +    D ILDGE+M+W+ N E  LR   + + T +  +K   E ++  L ++V+DV+Y+    G +A++    V K          LPL+ RR+LL RI++P        ++ E +LV  +      E + A + +  + G EGVI K  +  Y+  E  R     +KLKPDY  G   DLD +I+G  Y        +R+G +S FL+GV A  L        +++ AE  K     PV      +GTGY   EL +++ + E  W+ ++  ++P H    +   S L   V  WI P +S ++ V  +EL+     +    +RFPR + I  +K +Y         DL D    +  +S            +KR  +  LG  ++ A KR+K+   +    R P  ++   +  D+  +E++   FEG+   V+    + P Q                   K+ +E  +H  GG   Q  ++    +V+    +  +V  LK     K G    F+++   WV       R + +     + AT+     + K  DR+GDH+        + R L E    ++R  T  P       +KE+E   ++ ++      ++  V+  ++        DV   G   L+E     +G  V  E+ S  THV+   +        +R+ +HQ +   E  V+T+ WV++C+E
Sbjct:   57 YPLIRLVLPQLDRERTYM-LKEKKIAKIYIDVLGLAPISKDAQKLEHFNDPKIVDSKSVGDFAAV------LFDVVLYQSARTKSKTHHTLEDVNTLLDRLVQADNNAARK--KVFM--------------------------------KLATEYSADEQKWIIRVILKDLKIGLRHERVLKFIHPDAMEMYNHTNDLHKVCKE-------------LRNSAVRYVPQLEPFQVFTPMLAKRVNFGECISAINADA-----FVMEPKLDGERITCHV------QGK----EVQFISRNGINYTENYAPSIKPHVLSQLEPGIDCILDGEMMVWD-NTEYRLREFGLLKNTANAVRK--GEATNRWLCYVVWDVVYV--GGGPKAEQLIREVFKDPGEISAVMGLPLHARRKLLLRILKPLDHRI---IIIEQKLVNDKSAKERHEKVMAEVDQQVSNGGEGVIMKDLNAHYMCGESSRKAKKWLKLKPDYA-GMTTDLDVIIVGGFYGTGR----RRSGNVSVFLLGVLAHSLD-------EKKAAEATKPGAACPVVYTFAKVGTGYNLEELEQMRQELEPYWQPWDDDNVPSHLNGWKPQKSDLRPDV--WIDPRHSKILEVYGFELSFTTLYQTGLTIRFPRCKAIRNDKEWYQ------CIDLQDLNAARGSLS------------TKRASEVALG--QKSATKRIKKRQTV--SARRPNGVLQDYSRADLEGIEQERNVFEGMEFCVLPGKYQKPPQDLASSFPDGIFEKNAMQLSKQTVEKLLHSFGGAIVQNPIAGSTHYVVAAGDSGFKVVNLK-----KQG---LFNVVHIRWVFNCIVASRLVSLKAGDFISATDLQRQLLAKEYDRYGDHFTEHVTPDDLRRILQELSSASERKHTSLPW---QFKLKELEMEEAEAMDSAFTFLAHCVVYFHNSSSSEDKDIDVLAMGQMKLIEQQLKLYGGVVAREIDSSATHVVADPAQVEQLAPAIRNLRHQGLP--EPRVVTKEWVQECVE 957          

BLAST of Gcaud2574.t1 vs. uniprot
Match: M4B6V2_HYAAE (DNA ligase n=1 Tax=Hyaloperonospora arabidopsidis (strain Emoy2) TaxID=559515 RepID=M4B6V2_HYAAE)

HSP 1 Score: 206 bits (524), Expect = 5.530e-51
Identity = 257/1028 (25.00%), Postives = 426/1028 (41.44%), Query Frame = 0

Query:  102 LVSLIVPTLDSQHAYTGMRESRLADTFIKALDLNVNGEDAQWLKHYKEKEYRSSRCKYDADIVDGNLPTVIEAVL--KGRCPSKSSLTVGMVWNMLHTLSQTARKKSRRIGKVFYASAEQDNFAAPRLRAQTEDLSDPEDMRYDAIRKIVIHGTREEVAEVSRIILKDLDIRVREDSFLSWFHPAAKQHYTQMHDIQKLLQDCHDPKFEIGAPSVLVGSYASVMLTMRPSKRQLDVVCKNLRGNRGITASSSDAIDDSYFMMEPKLDGERMQLHKWTIESEQGRGLQTQIRTFSRRGNDSSAMYAEALNEVVLTAVR-AKDIILDGEIMIWNKNAEAWLRFEDMREITTSISKKKVPEGSSYILKFMVFDVLYIVQEDGDRAQKA----------ANMVMKLPLYQRRQLLKRIIQPRKASFCPGVLTEIELVEMEKGHNERELIQALQRYETL-GYEGVIAKHPDRPYVLAE--RNLNIAIKLKPDYFDGGIQDLDCLILGARYSDSHGHRVQRAGRLSSFLIGVRATDLTSTSSSKRDEEWAERMKKTKWIPV----GNIGTGYADSELAELQNKFEHDWKNFNLHDLPEHFER-REYTSTLLSGVAKWIQPWNSVVVTVTAYELN-----RRVWALRFPRVQRINWEKPYYDVPTFDHLKDLDDNKKPQTIVSDENDVDEVRETKSKRKRKNVLGSDEEDAVKRVKEEGHIVTGGRNPRTLISSAAGVDVSNLERKSTAFEG--------------------VCCHVITENPEQ--KENLEIKIHELGGTFSQTVSSGVDFVICTRATLQRVQMLKDLLSSKDGSNRTFSILRSTWVDESHSRLRKMVIDRSHVVHATEKLAADVYKVVDRFGDHWDRDADEHSIERALDEAKR----------WKTENPGYKDDVGIKEVEER--VSKVVEECKNIF---SNINVFVPHTQFDVSGTKILLEA----FGARVVNEVVSDCTHVLVHSSLRDKHQKIATA----------EETVITELWVEKCLE 1052
            L+ LI+P LD +  Y  ++E ++A  +I+ L L    +DAQ L+H+ + +   S+   D   V      + E VL   G    K   T+  V  +L TL Q     +R+                                  +   K+      EE   + RI+LKDL I +R +  L + HP A + Y   +D+ K+  D             L  S    +  + P +    ++ K +     I+A ++DA     F++EPKLDGER+  H   ++ E+       ++  SR G + +  YA ++   VL+ +    D ILDGE+M+W+ N E  LR   + + T +  +K   E ++  L ++V+DV+Y+    G +A++            + VM LPL  RR+LL RI++P      P    E +L+  +      E + A    + L G EG+I K  +  Y+  E  R     +KLKPDY  G   DLD LI+G  Y        +R+G +S FL+GV A  L        + E AE MK     P       +GTGY   EL +++ + E  W+ ++ +++P H    +   S L   V  WI P +S ++ V  +EL+     +    +RFPR + I  +K +Y         DL D    +  +S            +KR  +  LG  ++   KRVK+   +    R    ++      D+  +E     F+G                    +   +I E   Q  K+ +E  +H LGG   Q    G ++V+       +V  LK     K G    ++I+   WV    +  R +    S  V AT+     + +  D +GDH+         +  + E  R          W+ +      D+ ++E E        +  C   F   +N N  VP      +    LLE     +G  V +EV S  TH+++ S+ + +  ++  A          E  V+T+ WV++ +E
Sbjct:   55 LIRLILPQLDRERTYL-LKEKKIAKLYIQVLGLAPISKDAQKLEHFNDPKIADSKSVGDFAAV------LYEVVLYRSGASKLKPKHTIQDVNELLDTLVQADSNAARK----------------------------------NVFMKLATEYAAEEQKWIIRIVLKDLKIGLRHERVLKFIHPDAMEMYNHTNDLHKVCTD-------------LRNSAVRYVPQLEPFQVFTPMLAKRVNFGECISAINADA-----FVVEPKLDGERITCH---VQGEE-------VQFISRNGINYTESYAPSIKPHVLSQLELGVDCILDGEMMVWD-NTEYRLREFGLLKHTANAVRK--GEATNRWLCYVVWDVVYL---GGSKAEQLIRQVFQGPGEVSAVMGLPLVARRKLLLRILKPLDHRIIP---IEQKLINDKSAKERHEQVMAEVDQQVLNGGEGLIMKDLNAHYMCGESSRKSKKWLKLKPDYA-GMTTDLDTLIVGGLYGTGR----RRSGNVSVFLLGVLAHTLD-------ENEAAEAMKPGAQCPAVYTFAKVGTGYNLDELEQMRRELEPYWQPWDDNNIPPHLNGWKPQKSDLRPDV--WIDPRHSKILEVYGFELSFTTLYQTGLTIRFPRCKAIRNDKEWYQ------CIDLQDLNAARGSLS------------TKRASEVALG--QKSIAKRVKKRKTV--SPRRASGVLEEYTQADLDGIEPDRNLFKGKEFCVLPGKYEPPPPNVSLTIRRGIIDERTTQLSKQTVEKLLHALGGHVVQNPIEGTNYVVAAGDAGFKVANLK-----KQG---VYNIVHIRWVYSCTAASRLVSPKASDFVFATDSQRQALAREYDCYGDHFTEHIGPVDFKSIMQEVHREAGAESQALPWQLQMR----DLDMEEAESMSCAFTFLSHCVAYFQHCNNSNSSVPGANTIWASEAKLLEQQFRLYGGVVAHEVDSAVTHIIIDSTDQAQKAQLLPAIRALRHQGLPEPRVVTKEWVQQSVE 956          

BLAST of Gcaud2574.t1 vs. uniprot
Match: A0A425CAZ3_9STRA (DNA ligase n=1 Tax=Peronospora effusa TaxID=542832 RepID=A0A425CAZ3_9STRA)

HSP 1 Score: 200 bits (509), Expect = 3.340e-49
Identity = 246/992 (24.80%), Postives = 422/992 (42.54%), Query Frame = 0

Query:  100 FKLVSLIVPTLDSQHAYTGMRESRLADTFIKALDLNVNGEDAQWLKHYKEKEYRSSRCKYDADIVDGNLPTVIEAVLKGRCPSKSSL--TVGMVWNMLHTLSQTARKKSRRIGKVFYASAEQDNFAAPRLRAQTEDLSDPEDMRYDAIRKIVIHGTREEVAEVSRIILKDLDIRVREDSFLSWFHPAAKQHYTQMHDIQKLLQDCHDPKFEIGAPSVLVGSYASVMLTMRPSKRQLDVVCKNLRGNRGITASSSDAIDDSYFMMEPKLDGERMQLHKWTIESEQGRGLQTQIRTFSRRGNDSSAMYAEALNEVVLTAVR-AKDIILDGEIMIWNKNAEAWLRFEDMREITTSISKKKVPEGSSYILKFMVFDVLYIVQEDGDRAQKAANMVMKLPLYQRRQLLKRIIQPRKASFCPGVLTEIELVEMEKGHNERELIQA-LQRYETLGYEGVIAKHPDRPYVLAE--RNLNIAIKLKPDYFDGGIQDLDCLILGARYSDSHGHRVQRAGRLSSFLIGVRATDLTSTSSSKRDEEWAERMKKTKWIP----VGNIGTGYADSELAELQNKFEHDWKNFNLHDLPEHFER-REYTSTLLSGVAKWIQPWNSVVVTVTAYELN-----RRVWALRFPRVQRINWEKPYYDVPTFDHLKDLDDNKKPQTIVSDENDVDEVRETKSKRKRKNVLGSDEEDAVKRVKEEGHIVTGGRNPRTLISSAAGVDVSNLERKSTAFEG--------------------VCCHVITENPEQ--KENLEIKIHELGGTFSQTVSSGVDFVICTRATLQRVQMLKDLLSSKDGSNRTFSILRSTWVDESHSRLRKMVIDRSHVVHATEKLAADVYKVVDRFGDHWDRDADEHSIERALDEAKRWKTENPGYKDDVGIKEVEERVSKVVE----ECKNIF-SNINVFVPH--------------TQFDVSGTKILLEAFGARVVNEVVSDCTHVLV----HSSLRDK 1030
            + L+ L++P LD    Y  ++E ++A  +I+ L L    +DAQ L+H+ + +   S+   D   V      + E VL     SKS L  T+  V  +L+ L Q     +R+  KVF                                 K+    T EE   + R++LKDL I +R +  L + HP A + Y   +D+ K+  D             L  S    +  + P +    ++ K  R N G   S   AI+D  F++EPKLDGER+  H   ++ ++       ++  SR G + +  Y  ++   VL+ +    D ILDGE+M+W+ N E  LR   + + T + ++K   E ++  L ++V+DV+Y+    G    K      +  +  RR+LL RI++P        ++ E +L+  +   +  E + A + R  + G EGVI K+ +  Y+  E  R     +KLKPDY  G   DLD +I+G  Y        +R+G +S FL+GV A  L        + E AE MK     P       +GTGY   EL +++ + E  W+ ++  ++P H    +   S L   V  WI P +S ++ V  +EL+     +    +RFPR + I                    N K      D  D++  R + S  KR + +   ++   KRVK+   I    R P  ++   +  D++ +E++   FEG                    +   ++ E+ +Q  K+ +E  +H  GG   Q       +V+       +V  LK     K G    ++I+   W+ +  +  R + +  S  V AT      + +  DR+GDH+    +   ++R + +       N G + +V   +++ +  K+ E    +C   F S+  V+  H              T  +    K  L  +G  V +E+ S  TH+++    H S +D+
Sbjct:   55 YPLIRLVLPQLDRDRTYM-LKEKKIAKIYIQVLGLAPISKDAQKLEHFNDPKIVDSKSVGDFAAV------LYEVVLYRSGTSKSKLKHTIQDVNELLNYLVQADSNTARK--KVFM--------------------------------KLATEYTAEEQKWIIRVVLKDLKIGLRHERVLQFIHPDAMEMYNHTNDLHKVCTD-------------LRNSAVRYVPQLEPFQVFTPMLAK--RVNLGECIS---AINDDVFVVEPKLDGERITCH---VKGKE-------VQFISRNGTNYTENYGPSIKPHVLSQLELGVDCILDGEMMVWD-NTEYRLREFGLLKNTANAARK--GEATNRWLCYVVWDVVYL---GGGPKAKQLIRECRDGVTARRKLLLRILKPLDHRI---IINEQKLINDKSAKDRHEKVMAEVDRQVSNGGEGVIMKNLNAHYMCGESSRKAKKWLKLKPDYA-GMTTDLDAIIVGGFYGTGR----RRSGNVSVFLLGVLARSLD-------EREAAEAMKPGAPCPGVYTFAKVGTGYNLDELDQMRQELEPYWQPWDDDNIPSHLNCWKPQKSDLRPDV--WIDPRHSKILEVYGFELSFSTLYQTGLTIRFPRCKAIR-------------------NDKEWCQCIDLQDLNAARGSLST-KRASEVAFGQKSTTKRVKKRTTI--SARRPSGVLQGYSRADLNGIEQERNVFEGKEFCVLFGKYETPPPNIASTIPDGIVDEHAKQLSKQTVEKLVHSFGGNIMQNPIESTIYVVAAGDAGFKVVNLK-----KQG---LYNIVHIRWIFKCVAASRLVSLKASDFVFATALQREILARGYDRYGDHFTEHVNPEELKRIMRDV----CVNVGKEQEVSRWQIQIQDLKMEEAEAMDCPFTFLSHCVVYFHHCIRPDGSFAAENTVTAGETMFLKQQLRLYGGTVAHEIDSCVTHIVIETASHQSQKDR 920          

BLAST of Gcaud2574.t1 vs. uniprot
Match: UPI001BB13E40 (DNA ligase 4-like isoform X1 n=2 Tax=Lytechinus variegatus TaxID=7654 RepID=UPI001BB13E40)

HSP 1 Score: 195 bits (495), Expect = 1.780e-47
Identity = 222/1009 (22.00%), Postives = 406/1009 (40.24%), Query Frame = 0

Query:   84 HAQNIIPAHLFPPAESFKLVSLIVPTLDSQHAYTGMRESRLADTFIKALDLNVNGEDAQWLKHYKEKEYRSSRCKYDADIVDGNLPTVIEAVLKGRCPSKSSLTVGMVWNMLHTLSQTARKKSRRIGKVFYASAEQDNFAAPRLRAQTEDLSDPEDMRYDAIRKIVIHGTREEVAEVSRIILKDLDIRVREDSFLSWFHPAAKQHYTQMHDIQKLLQDCHDPKFEIGAPSVLVGSYASVMLTMRPSKRQLDVVCKNLRGNRGITASSSDAIDDSYFMMEPKLDGERMQLHKWTIESEQGRGLQTQIRTFSRRGNDSSAMYAEALNEVVLTAVRAK-------DIILDGEIMIWNKNAEAWLRFEDMREITTSISKKKVPEGSSYILKFMVFDVLYIVQEDGDRAQKAANMVMKLPLYQRRQLLKRIIQPRKASFCPGVLTEIE----LVEMEKGHNERELIQALQRYETLGYEGVIAKHPDRPYVLAERNLNIAIKLKPDYFDGGIQDLDCLILGARYSDSHGHRVQRAGRLSSFLIGVRATDLTSTSSSKRDEEWAERMKKTKWIPVGNIGTGYADSELAELQNKFEHDWKNFNLHDLPEHFERREYTSTLLSGVAKWIQPWNSVVVTVTAYELN-----RRVWALRFPRVQRINWEKPYYDVPTFDHLKDLDDNKKPQTIVSDENDVDEVRETKSKRKRKNVLGSDEEDAVKRVKEEGHIVTGGRNPRTLISSAAGVDVSNLERKSTAFEG-VCCHVITENPEQKENLEIKIHELGGTFSQTVSSGVDFVICTRATLQRVQMLKDLLSSKDGSNRTFSILRSTWVDESHSRLRKMVIDRSHVVHATEKLAADVYKVVDRFGDHWDRDADEHSIERALDEAKRWKTENPGYKDDVGIKEVEE----------RVSKVVEECKNIFSNINVFVPHTQFDVSGTKILLEAFGARVVNEVVSDCTHVLVHSS-------LRDKHQKIATAEETVITELWVEKCLEAGKILG 1058
            HA+N      F PA     + L++P L+ +    G++E  LA  +I  L L     DA+ L +Y     R+   K +A    G+  +V   VL+ RCP + SLT+  V + L +++     K + + K                                ++  ++   +  E   + R+I+K++ + + + S L  FHP A+  +   + + K+  D  DP   +   ++ V S  S ML  R    Q++ +                 ++   F++E KLDGERMQLHK   E ++          FSRR +D S  + ++  E  LT   A          ILDGE+  +N + +A++   +  +I      K + +     + + VFDVL +         K AN  +K                 + S+ P V T +E    LVE      + E+++AL        EG++ KHP+  Y   +R  +  +K+KP+Y D  + +LD L++G  +         RA  +S FL  V           K    + +            +G+GY   EL E   K    WK ++  + P   +       L      +++P NS +V + A E+N     +    LRFPR++++  +K ++   T + L+++    + +      N VD+     SKR++                    +      P  + S     D SN+++ S   +G   C + +     K  LE K+ E GG   Q        V+  +  L+    +K++ S+K      + ++++TW+ E       +    SH+++ + K+AA      D  GD +  D  E  ++       + K+E P   +++   E++           R+ +V  +   +    +  +     D+   +  +   GA +   +    TH++V  S        R + Q+ A  +  V+T  WV +C++  K++ 
Sbjct:   70 HAKNPKTTDNFYPA-----MRLLLPQLERERMAYGIKEHTLAKLYIDILGLAKESTDARKLLNY-----RAPTAKQEA----GDFASVAFFVLRNRCPERGSLTIKDVNDNLDSIALNNATKKKDLVK-------------------------------QSLLTLLRKTSAIEQKWLIRMIMKEMKVGLSQQSILGTFHPDAEDFFNVTNSLAKVCSDLSDPSVRLNEIAISVFSPFSPMLGERAKITQVERI-----------------MEHKSFLIETKLDGERMQLHK---EGDK-------YMYFSRRSHDYSGSFGKSPMEGSLTPYIANCFKSNVHSCILDGEMCAYNADTKAFISKGENFDI------KSLRDDEDVQVCYAVFDVLLV------NDTKLANRPLK----------------ERLSYLPKVFTVVEGRMVLVERRDAATKAEVVEALNDAIDRREEGIVVKHPESTYRPDKRKGSGWLKIKPEYVDNLMDELDLLVVGGYFGKGF-----RAQLISHFLCAVAVPPSREGDHPKIFYSFCK------------VGSGYTLQELYEFNQKLAKHWKKYDKENPPRWLQCTREKPEL------YVEPQNSFIVQIKATEINSSNVYKTGCTLRFPRLEKVRDDKDWHQCMTTEELENMRQQAEGKLTGRYAN-VDDDEIVTSKRRK--------------------VTARVERPVAVPSRFRAADTSNVKQTSEMLDGRELCIMNSPASHPKHELEKKVVECGGVVVQNPGIDTYCVLADKVNLK----VKNIFSAK-----IYDVVKTTWLIECLDAGHLLPWLPSHMLYTSAKMAAQFALDYDSHGDSYTEDVSEEKLKEIFATVGKKKSEKPMSAEEIAEIEMDYFPQDSHLGLFRLCRVYMDSCLVIGEEDTHIKDCSLDLISLE--MRFHGATMCPTLDRHVTHIIVDKSDLSRVREFRQRGQQRALKQH-VVTSDWVTECIQKEKLIS 922          

BLAST of Gcaud2574.t1 vs. uniprot
Match: A0A662X6F6_9STRA (DNA ligase n=2 Tax=Nothophytophthora sp. Chile5 TaxID=2483409 RepID=A0A662X6F6_9STRA)

HSP 1 Score: 194 bits (494), Expect = 3.340e-47
Identity = 258/1041 (24.78%), Postives = 434/1041 (41.69%), Query Frame = 0

Query:  100 FKLVSLIVPTLDSQHAYTGMRESRLADTFIKALDLNVNGEDAQWLKHYKEKEYRSSRCKYDADIVDGNLPTVIEAVLKGRCPSKSSLTVGMVWNMLHTLSQTARKKSRRIGKVFYASAEQDNFAAPRLRAQTEDLSDPEDMRYDAIRKIVIHGTREEVAEVSRIILKDLDIRVREDSFLSWFHPAAKQHYTQMHDIQKLLQDCHDPKFEIGAPSVLVGSYASVMLTMRPSKRQLDVVCKNLRGNRGITASSSDAIDDSYFMMEPKLDGERMQLHKWTIESEQGRGLQTQIRTFSRRGNDSSAMYAEALNEVVLTA-VRAKDIILDGEIMIWNKNAEAWLR-FEDMREITTSISKKKVPEGSSYILKFMVFDVLYIVQEDGDRAQKA----------ANMVMKLPLYQRRQLLKRIIQPRKASFCPGVLTEIELVEMEKGHNERELIQA-LQRYETLGYEGVIAKHPDRPYVLAE--RNLNIAIKLKPDYFDGGIQDLDCLILGARYSDSHGHRVQRAGRLSSFLIGVRATDLTSTSSSKRDEEWAERMKKTKWIPV----GNIGTGYADSELAELQNKFEHDWKNFNLHDLPEHF-ERREYTSTLLSGVAKWIQPWNSVVVTVTAYELN-----RRVWALRFPRVQRINWEKPYYDVPTFDHLKDLDDNKKPQTIVSDENDVDEVRETKSKRKRKNVLGSDEEDAVKRVKEEGHIVTGGRNPRTLISSAAGVDVSNLERKSTAFEG--------------------VCCHVITENPEQ--KENLEIKIHELGGTFSQT-VSSGVDFVICTRATLQRVQMLKDLLSSKDGSNRTFSILRSTWVDESHSRLRKMVIDRSHVVHATEKLAADVYKVVDRFGDHWDRDADEHSIERALDEAKRWK------TENPGYKDDVGIK------EVEERV---SKVVEECKNIFSNINVFVPHT---------QFDVSGTKILLEA----FGARVVNEVVSDCTHVLVHSS------------LRDKHQKIATAEETVITELWVEKCLE 1052
            + L+ L++P LD +  Y  ++E ++A  ++  L ++   +DAQ L+H+ + +   S+         G+   V+  VL+ R  + ++          HTL          + ++  A    D+ A+                R   + ++       E   V R++LKDL + +R +  L++ HP A + Y   +D+ K+  D             L  S    +  + P +    ++ K +      TA ++DA     F+MEPKLDGER+  H       QG+    Q++  SR G + +  Y  ++   VL+  V   D ILDGE+M+W+ N E  LR F  ++ +  ++      E +   L ++V+DV+Y+    G +A++            + VM LPL  RR+LL R++ P        V+ E  LV  +      +L+ A + R  + G EGVI K  +  Y+  E  R     +KLKPDY  G   +LD LI+G  Y        +R+G +S FL+GV A  L   S+       AE MK     P+      +GTGY   EL +++ + E  W+ ++  ++P H  + +   S L   V  WI P +S ++ V  +EL+     +    LRFPR + +  +K +Y         +L D    +  +S++   D     KS  KR          A KR      ++T  R+   ++++ +   +  LE +   FEG                    +   ++T+  ++  K+ +E  +H  GGT  Q  ++   ++V+       +V  LK     K G    F+I++  WV       R +    S  V ATE     + K  D FGDH+        + R L E  R        TE    +    I+      E EE +   S  +  CK  F     F   +         + D S    LLE     +G  +  E+ S  TH++V SS            +R    + A  E   +T  WV++ +E
Sbjct:   69 YPLMRLVLPQLDRERTYN-LKEKKIAKIYVDVLGMSSASKDAQKLEHFNDPKVVESKSV-------GDFAAVLYEVLRYRSLAGNT-------KREHTLQD--------VNELLDALVRADSNAS----------------RKKMVMRLATEFPANEQKWVVRVVLKDLKVGLRHERVLNFLHPDAMEMYNHTNDLHKVCTD-------------LRNSAVRYVPQLEPFQVFSPMLAKRVGFGECTTAINADA-----FVMEPKLDGERITCHV------QGK----QVQFISRNGINYTENYGPSMAPYVLSQLVSGVDCILDGEMMVWD-NLEYRLREFGLLKNVANAVRN---GEATHRWLCYVVWDVVYL--GGGPQAERLIREVYKGPGEVSAVMGLPLQARRKLLLRVLTPLDHRI---VIIEQTLVNSKSPKERHDLVMAEVDRQVSNGGEGVILKDLNAHYMCGESSRKTKKWLKLKPDYA-GMTTNLDALIIGGFYGTGR----RRSGNVSVFLLGVLAQSLDEHSA-------AEAMKPGAPCPIVYTFAKVGTGYNLEELEQMRQELEPHWQPWDSDNVPPHLNDWKPQKSDLKPDV--WIDPRHSKILEVYGFELSFTTLYQTGLTLRFPRCKAVRNDKEWYQ------CMNLQDLNAARGSISNKRAADVTLGQKSVLKR----------APKR-----QMLTA-RSAGGVLANYSQAKLEGLEMEQDVFEGKEFCVLPGKYEAPPPQLASAIPDEILTKQEKRLSKQTVEKLLHSFGGTIVQNPIAESTNYVVAALDAGFKVANLK-----KQG---LFNIVQLRWVLGCVGASRLLSPKASDFVFATEATRGLLAKEYDLFGDHFTEHVQPDDLRRILKEVARVTESSASATEAESDRQPWQIRRKAMAAEEEEAMDCESTFLSHCKVYFDRYPTFRTSSTRGEGDSEAEDDSSRRMELLEQQVRLYGGVISREIDSSVTHIVVASSSDRARIELLQPIIRSLRHRGAQ-EPPAVTVEWVQQSIE 988