Gcaud2443.t1 (polypeptide) Gracilaria caudata M_176_S67 male

BLAST of Gcaud2443.t1 vs. uniprot
Match: A0A2V3IVJ8_9FLOR (Very-long-chain enoyl-CoA reductase n=1 Tax=Gracilariopsis chorda TaxID=448386 RepID=A0A2V3IVJ8_9FLOR)

HSP 1 Score: 530 bits (1366), Expect = 9.950e-189
Identity = 243/303 (80.20%), Postives = 271/303 (89.44%), Query Frame = 0

Query:   28 MKVSVESRSGRDLGFYLLKDGSTVAVLQKSFHQAHSKYYPDRQRFYAKQDSSTAKKPAALSADDVLVDGCTLVFKDLGPQILWKTVFVVEYLGPLLLYPLFYSQPSWIYGGPYPPVSQAARDVQFAALIAWTFHYAKRELETLFIHRFSHATMPLRNLFKNCSYYWGFAAYVAYFVNHPLYTPPSVVNLSVGLCMFYFMEVGNFTAHWTLRTLRPPGTRIRKIPRGGLFELVSCPNYTFEIMSWLGFNIMTNTIPGIMFMLAGAFQMLLWAQGKHRRYKKEFDGKDDRPLYPPKRKILIPFIY 330
            MKVSV+SRSGRDLG + L+DGST++ LQ++FH AH KYYPDRQRFY KQD STAKKP ALS++D LVD CTLVFKDLGPQILWKTVF+VEYLGPLLLYPLFY+QP+W+YG   PP S AAR+VQ AAL+AWTFHYAKRE ETLFIHRFSHATMPLRN+FKN  YYWGFAAYVAYFVNHPLYTPP+   L+VGLC+FYFMEVGNFTAH TLR LRP GTR+RKIPRGGLFE VSCPNYTFEIM+WLGFN+MT T+ GI+FM AGAFQM+LWAQGKHRRYKKEFDG+D RPLYPP RKI+ PF+Y
Sbjct:    1 MKVSVQSRSGRDLGTFTLEDGSTLSALQEAFHSAHKKYYPDRQRFYGKQDPSTAKKPVALSSEDALVDDCTLVFKDLGPQILWKTVFIVEYLGPLLLYPLFYTQPAWMYGTTLPPSSDAAREVQMAALMAWTFHYAKREFETLFIHRFSHATMPLRNIFKNSFYYWGFAAYVAYFVNHPLYTPPTQQYLAVGLCVFYFMEVGNFTAHLTLRNLRPAGTRVRKIPRGGLFEFVSCPNYTFEIMAWLGFNVMTRTVAGILFMCAGAFQMILWAQGKHRRYKKEFDGQDGRPLYPPNRKIIFPFVY 303          

BLAST of Gcaud2443.t1 vs. uniprot
Match: R7Q6X5_CHOCR (Trans-2-enoyl-CoA reductase (NADPH) n=1 Tax=Chondrus crispus TaxID=2769 RepID=R7Q6X5_CHOCR)

HSP 1 Score: 464 bits (1193), Expect = 2.260e-162
Identity = 218/304 (71.71%), Postives = 250/304 (82.24%), Query Frame = 0

Query:   28 MKVSVESRSGRDLGFYLLKDGSTVAVLQKSFHQAHSKYYPDRQRFYAKQDSSTAKKPAALSADDVLVDGCTLVFKDLGPQILWKTVFVVEYLGPLLLYPLFYSQPSWIYGGP-YPPVSQAARDVQFAALIAWTFHYAKRELETLFIHRFSHATMPLRNLFKNCSYYWGFAAYVAYFVNHPLYTPPSVVNLSVGLCMFYFMEVGNFTAHWTLRTLRPPGTRIRKIPRGGLFELVSCPNYTFEIMSWLGFNIMTNTIPGIMFMLAGAFQMLLWAQGKHRRYKKEFDGKDDRPLYPPKRKILIPFIY 330
            MKVSV+S SGR LG Y L   +T++ LQ  FH AH  YYPDRQRFY    + +A +P AL+ D  L D   L+FKDLGPQILWKTVF++EYLGPLLLYPLFY+QP WIYG   +  V+  AR+VQF+AL+AWTFHYAKRELETLF+HRFSHATMPL NLFKN  YYWGFAAYVAYFVNHPLYTPP    +  G+C+FYFMEVGN +AHWTLR LRPPGT++R+IPRGGLFELVSCPNYT+EI+SWLGFN+MT TI G++FM AGAFQML+WA  KHRRYKKEFDGKD R LYPP RKILIPF+Y
Sbjct:    1 MKVSVQSTSGRPLGDYQLPASATLSSLQPLFHAAHPLYYPDRQRFYPSPAAPSATRPPALNPDSPLSDADVLLFKDLGPQILWKTVFLLEYLGPLLLYPLFYNQPRWIYGDAIHAAVTPWAREVQFSALMAWTFHYAKRELETLFVHRFSHATMPLSNLFKNSIYYWGFAAYVAYFVNHPLYTPPKEDLVYTGMCLFYFMEVGNLSAHWTLRMLRPPGTKVRRIPRGGLFELVSCPNYTYEILSWLGFNMMTKTIAGLLFMCAGAFQMLVWAGSKHRRYKKEFDGKDGRALYPPSRKILIPFLY 304          

BLAST of Gcaud2443.t1 vs. uniprot
Match: A0A7S0BNR2_9RHOD (Hypothetical protein n=2 Tax=Rhodosorus marinus TaxID=101924 RepID=A0A7S0BNR2_9RHOD)

HSP 1 Score: 330 bits (847), Expect = 7.650e-110
Identity = 158/305 (51.80%), Postives = 216/305 (70.82%), Query Frame = 0

Query:   28 MKVSVESRSGRDLGFYLLKDGSTVAVLQKSFHQAHSKYYPDRQRFYAKQDSSTAKKPAALSADDVL---VDGCTLVFKDLGPQILWKTVFVVEYLGPLLLYPLFYSQPSWIYGGPYPPVSQAARDVQFAALIAWTFHYAKRELETLFIHRFSHATMPLRNLFKNCSYYWGFAAYVAYFVNHPLYTPPSVVNLSVGLCMFYFMEVGNFTAHWTLRTLRPPGTRIRKIPRGGLFELVSCPNYTFEIMSWLGFNIMTNTIPGIMFMLAGAFQMLLWAQGKHRRYKKEFDGKDDRPLYPPKRKILIPFI 329
            M +++ SRSGR +    L D + +  L+ +FH+ ++KYYPDRQRF     +S ++KP ++  D  L   +   T+VFKDLGPQI W++VFV+EYLGPLL++P+F  +P +IYG   PP+ +     Q++  +A+ FHY KRELETL +H+FS++TMP+ N+FKN  YYWGFAA + YF+ HPLYTP ++   +  + MF+F EV NF  H  L +LRP GTR+RKIPR G FE VSCPNY FEI++W+ FN+ T+T+ G  F +AG+ QML+WA  KH+ Y+KEFDGKD R LYP  RK LIPFI
Sbjct:    1 MDLTIVSRSGRKIAQVSLPDEAKLRDLKDAFHKTNTKYYPDRQRF-TLPTTSASEKPLSVLEDIPLKEQLSDATVVFKDLGPQISWRSVFVIEYLGPLLIFPIFLMRPWFIYGTDLPPLIEQ----QYSGFVAFLFHYVKRELETLLVHKFSNSTMPIMNVFKNSIYYWGFAAGIGYFLFHPLYTPVAMGRYAFCMAMFWFWEVANFVTHAYLSSLRPAGTRVRKIPRDGAFEFVSCPNYLFEILAWMSFNLFTSTLVGWAFFVAGSAQMLVWAMKKHKNYQKEFDGKDGRELYPKGRKALIPFI 300          

BLAST of Gcaud2443.t1 vs. uniprot
Match: A0A1X6NID4_PORUM (Uncharacterized protein n=1 Tax=Porphyra umbilicalis TaxID=2786 RepID=A0A1X6NID4_PORUM)

HSP 1 Score: 328 bits (841), Expect = 7.110e-109
Identity = 171/308 (55.52%), Postives = 197/308 (63.96%), Query Frame = 0

Query:   28 MKVSVESRSGRDLGFYLLKDGSTVAVLQKSFHQAHSKYYPDRQRFYAKQDSSTAKKPAALSADDVLV-----DGCTLVFKDLGPQILWKTVFVVEYLGPLLLYPLFYSQPSWIYGGPYPPVSQAARDVQFAALIAWTFHYAKRELETLFIHRFSHATMPLRNLFKNCSYYWGFAAYVAYFVNHPLYTPPSVVNLSVGLCMFYFMEVGNFTAHWTLRTLRPPGTRIRKIPRGGLFELVSCPNYTFEIMSWLGFNIMTNTIPGIMFMLAGAFQMLLWAQGKHRRYKKEFDGKDDRPLYPPKRKILIPFIY 330
            MK+ V+SRSG+ LG   L + + V  L+ ++ +     Y DRQRFY         KP AL     L      DG T+VFKDLGPQI WK VFV+EYLGPLL++P F      IYG   PP S AA   Q A  +AW  HYAKRE ETL +HRFSHATMPL NL KNC YYWGFAA VAYF  HPLYTP S      GL  FYFME GN   H+ LR LRP G+ +R+IP GG F  VSCPNYT EI +W+ FN+   T+ G  F   GA QM +WA GKHR Y+KEFDGKD RPLYP  RK LIPFI+
Sbjct:    1 MKIHVQSRSGKPLGTLTLPNDADVPALKAAYAKQTPSMYVDRQRFYLPAVGGA--KPVALGDRATLAAAGVADGGTVVFKDLGPQISWKAVFVLEYLGPLLIFPAFLLGQRAIYGRVTPPGSVAAEH-QVALALAWALHYAKREFETLCVHRFSHATMPLGNLAKNCGYYWGFAAAVAYFAMHPLYTPASDAAYISGLVTFYFMEAGNMWCHFKLRALRPAGSTVRRIPMGGPFSYVSCPNYTCEIGAWVAFNVACPTVWGWAFAACGAAQMAVWAAGKHRNYRKEFDGKDGRPLYPRSRKALIPFIW 305          

BLAST of Gcaud2443.t1 vs. uniprot
Match: UPI0006449B43 (hypothetical protein n=1 Tax=Acytostelium subglobosum LB1 TaxID=1410327 RepID=UPI0006449B43)

HSP 1 Score: 313 bits (803), Expect = 3.940e-103
Identity = 162/309 (52.43%), Postives = 202/309 (65.37%), Query Frame = 0

Query:   28 MKVS-VESRSGRDLGFYLLKDGSTVAVLQKSFHQAHSKYYPDRQRFYAKQDSSTAKKPAALSADDVLVD------GCTLVFKDLGPQILWKTVFVVEYLGPLLLYPLFYSQPSWIYGGPYPPVSQAARDVQFAALIAWTFHYAKRELETLFIHRFSHATMPLRNLFKNCSYYWGFAAYVAYFVNHPLYTPPSVVNLSVGLCMFYFMEVGNFTAHWTLRTLRPPGTRIRKIPRGGLFELVSCPNYTFEIMSWLGFNIMTNTIPGIMFMLAGAFQMLLWAQGKHRRYKKEFDGKDDRPLYPPKRKILIPFI 329
            MK++ V SR  + +     +  STV  L+KSF + + KYY DRQRF      +  K P AL+ D   +         TL+FKDLGPQI W  VF+VEY GPL +YPLFY     IYG  +         VQ  ALI ++ HY KR LET+F+HRFSH TMPL NLFKNC+YYWG    V+YFVNH LYT PS +N+ VGL +F   E  N   H  LR LRPPG+  R+IP+GGLF+LVSCPNYT EI +W+GF+IMT T+   +F L GAFQM +WA  KHRRY+KEFDG++  PLYP  RKI++PF+
Sbjct:    1 MKITIVTSRGNKHICDIEAETTSTVLQLKKSFEKKNKKYYVDRQRFTTSTLGADGK-PVALTDDGKTLSHYNIKSDTTLLFKDLGPQISWTLVFLVEYAGPLCIYPLFYIFSKQIYGTDFQK-----SYVQDLALICYSLHYIKRLLETIFVHRFSHGTMPLSNLFKNCTYYWGCTIMVSYFVNHALYTAPSSINVFVGLVLFAVSEFLNLMCHIQLRNLRPPGSTERRIPKGGLFDLVSCPNYTAEITAWIGFSIMTQTLTAFIFTLLGAFQMYVWAVAKHRRYRKEFDGQNGNPLYPRNRKIIVPFL 303          

BLAST of Gcaud2443.t1 vs. uniprot
Match: A0A7J6MXZ0_PEROL (Uncharacterized protein n=1 Tax=Perkinsus olseni TaxID=32597 RepID=A0A7J6MXZ0_PEROL)

HSP 1 Score: 308 bits (789), Expect = 1.150e-100
Identity = 160/328 (48.78%), Postives = 210/328 (64.02%), Query Frame = 0

Query:   28 MKVSVESRSGRDLGFYLLKDGS-TVAVLQKSFHQAHSKYYPDRQRFYAKQDSSTAKK---PAALSADDV------LVDGCTLVFKDLGPQILWKTVFVVEYLGPLLLYPLFYSQPSWIYG----GPYPPVSQA-----------ARDVQFAALIAWTFHYAKRELETLFIHRFSHATMPLRNLFKNCSYYWGFAAYVAYFVNHPLYTPPSVV-NLSVGLCMFYFMEVGNFTAHWTLRTLRPPGTRIRKIPRGGLFELVSCPNYTFEIMSWLGFNIMTNTIPGIMFMLAGAFQMLLWAQGKHRRYKKEFDGKDDRPLYPPKRKILIPFI 329
            MK++V+SR G++L    + + S TVA L+K   +A  K    RQR      S + KK   P AL+ D        L DG  +VFKDLGPQI W+ VF +EY GP++++P+ Y    +IYG    G +P VS A            R+ Q  A + W  H+ KRE ETLF+HRFS+ TMPLRNLFKNC+YYWGFA  V Y+  HPLYTP +     ++GL +F   E+ N+  H  LR LRP G+R+RK+P G  FELVSCPNY FEI+SW GFN+M++T+ G+ +MLAGA QM +WA  KHR Y++EFDGKD R LYP  RKI+IP++
Sbjct:    1 MKITVQSRKGKELAVVTVPEKSATVAELKKGLTKA-IKTSEARQRLTLPLSSDSTKKGSKPVALTNDSATLSSYGLKDGDVVVFKDLGPQIAWRLVFFIEYFGPMVIFPILYFGQMFIYGATSPGDHPSVSDAFKHFVAAPTVSVREAQLVATVCWFLHFLKREYETLFVHRFSNGTMPLRNLFKNCTYYWGFALLVGYYACHPLYTPVAEQWQRALGLAIFTVCEICNYRCHAILRDLRPKGSRVRKVPHGFAFELVSCPNYMFEILSWFGFNLMSHTVMGVAYMLAGAAQMWVWAVKKHRNYRREFDGKDGRELYPKNRKIIIPYL 327          

BLAST of Gcaud2443.t1 vs. uniprot
Match: C5LH77_PERM5 (Synaptic glycoprotein sc2, putative n=2 Tax=Perkinsus marinus (strain ATCC 50983 / TXsc) TaxID=423536 RepID=C5LH77_PERM5)

HSP 1 Score: 308 bits (789), Expect = 1.150e-100
Identity = 160/328 (48.78%), Postives = 207/328 (63.11%), Query Frame = 0

Query:   28 MKVSVESRSGRDLGFYLLK-DGSTVAVLQKSFHQAHSKYYPDRQRFYAKQDSSTAKK---PAALSADDV------LVDGCTLVFKDLGPQILWKTVFVVEYLGPLLLYPLFYSQPSWIYG----GPYPPVSQA-----------ARDVQFAALIAWTFHYAKRELETLFIHRFSHATMPLRNLFKNCSYYWGFAAYVAYFVNHPLYTPPSVV-NLSVGLCMFYFMEVGNFTAHWTLRTLRPPGTRIRKIPRGGLFELVSCPNYTFEIMSWLGFNIMTNTIPGIMFMLAGAFQMLLWAQGKHRRYKKEFDGKDDRPLYPPKRKILIPFI 329
            MKV+V++R G++L    +  D +TVA L++    A  K    RQR      + +AKK   P AL+ D        L DG  +VFKDLGPQI W+ VF +EY GP++++P+ Y     +YG    G +P   +A            R+ Q  A + W  H+ KRE ETLF+HRFS+ TMP+RNLFKNC+YYWGFA  V YFV HPLYTP +      +GL +F   E+ N+  H  LR LRP G+R+RK+P G  FELVSCPNY FEI+SW GFN+MT T+ GI +MLAGA QM +WA  KHR Y++EFDGKD R LYP  RKILIP++
Sbjct:    1 MKVTVQNRRGKELAVITVSGDAATVAELKQGIESA-MKTSEARQRLTLPLSADSAKKGSKPVALTDDSATLSSYGLADGDVIVFKDLGPQISWRLVFFIEYFGPMVIFPILYYGQKHVYGAVSPGGHPSFQEALRHFVAAPTVSVREAQLVASVCWFLHFLKREYETLFVHRFSNGTMPIRNLFKNCAYYWGFALLVGYFVCHPLYTPAAQQWQRILGLAIFAVCEISNYRCHAILRDLRPKGSRVRKVPHGFAFELVSCPNYMFEILSWFGFNMMTQTVMGIAYMLAGAGQMWVWALKKHRNYRREFDGKDGRELYPKNRKILIPYL 327          

BLAST of Gcaud2443.t1 vs. uniprot
Match: UPI0008F9B750 (probable very-long-chain enoyl-CoA reductase art-1 n=1 Tax=Bemisia tabaci TaxID=7038 RepID=UPI0008F9B750)

HSP 1 Score: 307 bits (786), Expect = 1.300e-100
Identity = 157/308 (50.97%), Postives = 197/308 (63.96%), Query Frame = 0

Query:   28 MKVSVESRSGRDLGFYLLKDGSTVAVLQKSFHQAHSKYYPDRQRFYAKQDSSTAKKPAALSADDVLVDGCTLVFKDLGPQILWKTVFVVEYLGPLLLYPLFYSQPSWIYGGPYPPVSQAARDVQFAALIAWTFHYAKRELETLFIHRFSHATMPLRNLFKNCSYYWGFAAYVAYFVNHPLYTPPSVVNLSVGLCMFYFMEVGNFTAHWTLRTLRPPGTRIRKIPRGG------LFELVSCPNYTFEIMSWLGFNIMTNTIPGIMFMLAGAFQMLLWAQGKHRRYKKEFDGKDDRPLYPPKRKILIPFI 329
            MKV + + SG+ +G   L   STV  ++K  ++   + YP+RQ    +     AK    L    +  DG  L  KDLGPQI W TVF+ EY GPL  Y LFY +PS+IYG      S     V   A I W+ HYAKR LET+F+HRFS+ATMPL NLFKNCSYYWGFAA+V+YF NHPLYTPPS V + VGL  F   E+GN + H  LR LRPPGT++RKIP         LF LVSCPNYT+EI SW+ F++MT ++P ++F +AGA+QM +WA GKHR YKKEF        YP +RK + PF+
Sbjct:    1 MKVELLTTSGKPIGAVELSKASTVQDVKKEVYKIKKQLYPERQSIRLEVKGKGAKDSDTLENLGIQ-DGGALYVKDLGPQIGWSTVFLSEYAGPLFTYLLFYPRPSFIYGAD--AASSKYECVVTIAAICWSVHYAKRILETIFVHRFSNATMPLSNLFKNCSYYWGFAAFVSYFTNHPLYTPPSDVQMKVGLAAFLICELGNLSIHIALRNLRPPGTKVRKIPVATGNPFTILFSLVSCPNYTYEIGSWIAFSVMTGSLPALIFTIAGAYQMTIWALGKHRNYKKEFS------TYPKQRKAIFPFL 299          

BLAST of Gcaud2443.t1 vs. uniprot
Match: L8GUP0_ACACA (Glycoprotein, synaptic 2 family protein n=1 Tax=Acanthamoeba castellanii str. Neff TaxID=1257118 RepID=L8GUP0_ACACA)

HSP 1 Score: 305 bits (781), Expect = 6.940e-100
Identity = 157/308 (50.97%), Postives = 200/308 (64.94%), Query Frame = 0

Query:   29 KVSVESRSGRDLGFYLLKDGSTVAVLQKSFHQAHSKYYPDRQRFYAKQDSSTA---KKPAALSADDVLVD--GCTLVFKDLGPQILWKTVFVVEYLGPLLLYPLFYSQPSWIYGGPYPPVSQAARD-VQFAALIAWTFHYAKRELETLFIHRFSHATMPLRNLFKNCSYYWGFAAYVAYFVNHPLYTPPSVVNLSVGLCMFYFMEVGNFTAHWTLRTLRPPGTRIRKIPRGGLFELVSCPNYTFEIMSWLGFNIMTNTIPGIMFMLAGAFQMLLWAQGKHRRYKKEFDGKDDRPLYPPKRKILIPFIY 330
            K++++S SG+ L    + + STVA L+ ++ +A  KYYPDRQRFY  QD  T    +    LS  ++L       + F+DLGPQI WKTVF+VEY GPLL+Y  FY++P +IY     P S A    VQ  AL+ W  HY KRELET+F+HRFSH TMP+ N+FKN  YYWGF A   YFVNHP +T P    +   L  F   E+GN  +H  LR LRP GT +R+IPRG LFELVSCPNYT EI++W+ F++MT ++   +F L G  QML+WAQ KHRRYKKEF G      YP  R+IL PF+Y
Sbjct:    3 KLTIKSPSGKVLKELEVSESSTVAQLKAAYAKAFPKYYPDRQRFYL-QDGETRVSLEDEKKLSTYNLLCSKRDDVVYFRDLGPQIAWKTVFLVEYAGPLLIYLFFYARPPFIY-----PASDAPHTWVQNLALLCWAGHYLKRELETIFVHRFSHGTMPIMNIFKNSGYYWGFGALCGYFVNHPYFTNPEPTQVYTALAFFVLFELGNLISHIQLRNLRPEGTTVRRIPRGFLFELVSCPNYTCEILAWVCFSLMTQSVAAFLFTLVGGGQMLVWAQQKHRRYKKEFPG------YPKNRRILFPFLY 298          

BLAST of Gcaud2443.t1 vs. uniprot
Match: UPI000D7342C7 (very-long-chain enoyl-CoA reductase-like n=1 Tax=Pomacea canaliculata TaxID=400727 RepID=UPI000D7342C7)

HSP 1 Score: 302 bits (774), Expect = 1.440e-98
Identity = 160/319 (50.16%), Postives = 210/319 (65.83%), Query Frame = 0

Query:   24 SDANMKVS-VESRSG---RDLGFYLLKDGSTVAVLQKSFHQAHSKYYPDRQRFYAKQDSSTAKKPAALSADDVLVDGCTLVFKDLGPQILWKTVFVVEYLGPLLLYPLFYSQPSWIYGGPYPPVSQAARDVQFAALIAWTFHYAKRELETLFIHRFSHATMPLRNLFKNCSYYWGFAAYVAYFVNHPLYTPPSVVNLSV--GLCMFYFMEVGNFTAHWTLRTLRPPGTRIRKIPRGG------LFELVSCPNYTFEIMSWLGFNIMTNTIPGIMFMLAGAFQMLLWAQGKHRRYKKEFDGKDDRPLYPPKRKILIPFIY 330
            +D+ M++  V SRSG     LG    K  STV  ++K+F +  SKYYPDRQ F  +      K    L +  +  +G  L FKDLGPQ+ W TVF+ EY GPL++Y  FY++P+ IYG     +  A + V  AA   WTFHYAKR LET+F+HRFSHATMP+RN+FKN  YYWGFAA+VAYFVNHPLYTPP+  ++ V  GL +F   E+GNF+ H  LR+LRPPGT+ RKIP         LF LVSCPNYT+E++SW+ F++MT  +P  +F LAG +QM +WA+GKH  Y+KEF        YP  RK ++PF++
Sbjct:    9 ADSTMEIQIVHSRSGDIITTLGDLHAK--STVGDIKKAFFKEKSKYYPDRQSFRTEPRGKVIKDTETLESLGIK-NGGKLYFKDLGPQVGWTTVFLTEYAGPLVIYLAFYTRPALIYGAEAATLPYA-KVVNIAAG-CWTFHYAKRLLETIFVHRFSHATMPIRNIFKNSLYYWGFAAFVAYFVNHPLYTPPAYGDIQVYGGLAVFALSELGNFSIHLALRSLRPPGTKERKIPYATSNPFTWLFSLVSCPNYTYEVLSWIAFSVMTQCLPAALFTLAGFYQMAVWAKGKHHNYRKEFSN------YPRSRKPIVPFLF 316