Gcaud2259.t1 (polypeptide) Gracilaria caudata M_176_S67 male

BLAST of Gcaud2259.t1 vs. uniprot
Match: A0A2V3J542_9FLOR (Asparagine--tRNA ligase n=1 Tax=Gracilariopsis chorda TaxID=448386 RepID=A0A2V3J542_9FLOR)

HSP 1 Score: 981 bits (2535), Expect = 0.000e+0
Identity = 473/521 (90.79%), Postives = 499/521 (95.78%), Query Frame = 0

Query:   12 EEGEFHKMTLDTFVAMSEDDRAKMSATARKKWLKLEKKAQDKSRKEEEAXXXXAAENAARLERAKQIKIEQDPSLPEAKQVKIRDLGKYVDQRVKVYGWVHHLRADGKKLWFIDLRDGTGFAQVVLTGNMCMTYDAATLYREAAIAVYGKWIKDEQGRAQGSFPGFELAADYWELVGASCADIDMLFTKDSNPDILMNQRHLVLRGSKASSIMRMRSIITHCFREHFFDRGYTELSPPTLVNTQCEGGSTLFNFKYFEDQGYLTQSSQLYLETGIAAVNDCFCILPSYRAEKSQTRRHLAEFHHIEAERPFITFEDLLETIEDLVCDVIDRVGKKMEGEMDTFLRASAGKPIPKLERPFLRMTYEDAVQYCRDHEIYKDPETKEHFEFGDDIPEKPEREMTDQINRPIMLIKFPASLKSFYMQKCEDDKRLTESVDLLLPGVGEVVGGSMRMWNADELMEAYKKENMDPAPYYWFTDQRKYGSCPHGGYGLGLERFICALLGIHHIRDACLYPRYRGRISP 532
            EE +F KMTLDTF+ MS+D+R+K+SATARKKW+KLEKKAQDKSRK    XXX AAENAARLERAKQIKIE D SLP AKQ+KIRDL KYVDQRVK+YGWVHHLR DGKKLWFIDLRDGTGFAQ+VLTG MCMTY+AATLYREAAIAVYGKW+KDE+GRAQG+FPG+ELAAD+WELVG SCADIDMLFTKDSNPDILMNQRHLVLRGSKASS+MRMRSIITHCFREHFFDRGYTELSPPTLVNTQCEGGSTLFNF YFED GYLTQSSQLYLETGIAAVNDCFCILPSYRAEKSQTRRHLAEFHHIEAERPFITFEDLLETIEDLVCDVIDRVGKKME +MDTFLRAS GKPIPKLERPFLRMTYEDAV+YCR+H IYKDPETKEHFEFGDDIPEKPER+MTD+INRPIMLIKFPASLKSFYMQKC+DDKRLTESVDLLLPGVGEVVGGSMRMW+ DELMEAYKKE MDPAPYYWFTDQRKYG+CPHGGYGLGLERFICALLGIHHIRDACLYPRYRGRI+P
Sbjct:   11 EEPDFQKMTLDTFMKMSDDERSKLSATARKKWIKLEKKAQDKSRKXXXXXXXAAAENAARLERAKQIKIELDLSLPPAKQLKIRDLSKYVDQRVKIYGWVHHLRTDGKKLWFIDLRDGTGFAQIVLTGEMCMTYEAATLYREAAIAVYGKWVKDEKGRAQGTFPGYELAADFWELVGPSCADIDMLFTKDSNPDILMNQRHLVLRGSKASSVMRMRSIITHCFREHFFDRGYTELSPPTLVNTQCEGGSTLFNFNYFEDDGYLTQSSQLYLETGIAAVNDCFCILPSYRAEKSQTRRHLAEFHHIEAERPFITFEDLLETIEDLVCDVIDRVGKKMESDMDTFLRASGGKPIPKLERPFLRMTYEDAVKYCREHGIYKDPETKEHFEFGDDIPEKPERQMTDEINRPIMLIKFPASLKSFYMQKCDDDKRLTESVDLLLPGVGEVVGGSMRMWDMDELMEAYKKEGMDPAPYYWFTDQRKYGTCPHGGYGLGLERFICALLGIHHIRDACLYPRYRGRIAP 531          

BLAST of Gcaud2259.t1 vs. uniprot
Match: R7QDE8_CHOCR (Asparagine--tRNA ligase (Fragment) n=1 Tax=Chondrus crispus TaxID=2769 RepID=R7QDE8_CHOCR)

HSP 1 Score: 830 bits (2144), Expect = 8.130e-301
Identity = 399/485 (82.27%), Postives = 434/485 (89.48%), Query Frame = 0

Query:   48 KKAQDKSRKEEEAXXXXAAENAARLERAKQIKIEQDPSLPEAKQVKIRDLGKYVDQRVKVYGWVHHLRADGKKLWFIDLRDGTGFAQVVLTGNMCMTYDAATLYREAAIAVYGKWIKDEQGRAQGSFPGFELAADYWELVGASCADIDMLFTKDSNPDILMNQRHLVLRGSKASSIMRMRSIITHCFREHFFDRGYTELSPPTLVNTQCEGGSTLFNFKYFEDQGYLTQSSQLYLETGIAAVNDCFCILPSYRAEKSQTRRHLAEFHHIEAERPFITFEDLLETIEDLVCDVIDRVGKKMEGEMDTFLRASAGKPIPKLERPFLRMTYEDAVQYCRDHEIYKDPETKEHFEFGDDIPEKPEREMTDQINRPIMLIKFPASLKSFYMQKCEDDKRLTESVDLLLPGVGEVVGGSMRMWNADELMEAYKKENMDPAPYYWFTDQRKYGSCPHGGYGLGLERFICALLGIHHIRDACLYPRYRGRISP 532
            KKA  KS KE EA    AAENAARLERA+ + I +DPSLP A+  KIRDL + +  RVKV GWVHHLR DGKKLWFIDLRDGTGFAQVVLTG++C TYDAATL REA+IAVYG W KD+ GRA+G++PGFEL  DYW+LVGA+ +DIDMLFTKDSN DIL+NQRHLVLRGSKASSI+RMRSIIT CFREHFF   Y EL+PPTLVNTQCEGGSTLF F YF ++GYLTQSSQLYLETGIAAVNDCFCILPSYRAEKSQTRRHL+EFHHIEAERPFITFEDLL++IE LVC VIDRV  KM+ +MDTFLRAS GKP+PKLERPF+RMTYE+AV YCR+H IYKDPETK HFEFGDDIPEKPEREMTD+IN+PI+LIKFPASLKSFYMQKC DD RLTESVDLLLPGVGEVVGGSMRMW ADEL+EAYKKENMDP PYYWFTDQRKYGSCPHGGYGLGLERFICALL IHHIRDACLYPRYRGRI+P
Sbjct:    1 KKAALKSEKEAEAAARAAAENAARLERARAVVIAEDPSLPPARPAKIRDLPRCIGARVKVCGWVHHLRTDGKKLWFIDLRDGTGFAQVVLTGDLCRTYDAATLGREASIAVYGVWTKDDNGRAKGTWPGFELQVDYWQLVGATSSDIDMLFTKDSNVDILLNQRHLVLRGSKASSILRMRSIITQCFREHFFAEHYVELTPPTLVNTQCEGGSTLFAFDYFGEEGYLTQSSQLYLETGIAAVNDCFCILPSYRAEKSQTRRHLSEFHHIEAERPFITFEDLLQSIEGLVCGVIDRVADKMKDDMDTFLRASGGKPMPKLERPFMRMTYEEAVAYCREHNIYKDPETKTHFEFGDDIPEKPEREMTDKINKPILLIKFPASLKSFYMQKCPDDTRLTESVDLLLPGVGEVVGGSMRMWKADELLEAYKKENMDPEPYYWFTDQRKYGSCPHGGYGLGLERFICALLNIHHIRDACLYPRYRGRIAP 485          

BLAST of Gcaud2259.t1 vs. uniprot
Match: A0A5J4YQD6_PORPP (Asparagine--tRNA ligase n=1 Tax=Porphyridium purpureum TaxID=35688 RepID=A0A5J4YQD6_PORPP)

HSP 1 Score: 649 bits (1673), Expect = 1.470e-228
Identity = 327/526 (62.17%), Postives = 396/526 (75.29%), Query Frame = 0

Query:   14 GEFHKMTLDTFVAMSEDDRAKMSATARKKWLKLEKKAQDKSRKEEEAXXXXAAENAARLERAKQIKIEQDPSLPEAKQVKIRDL-------GKYVDQRVKVYGWVHHLRADGKKLWFIDLRDGTGFAQVVLTGNMCMTYDAATLYREAAIAVYGKWIKDEQGRAQGSFPGFELAADYWELVGASCADIDMLFTKDSNPDILMNQRHLVLRGSKASSIMRMRSIITHCFREHFFDRGYTELSPPTLVNTQCEGGSTLFNFKYFEDQGYLTQSSQLYLETGIAAVNDCFCILPSYRAEKSQTRRHLAEFHHIEAERPFITFEDLLETIEDLVCDVIDRVGKKMEGEMDTFLRASAGKPIPKLERPFLRMTYEDAVQYCRDHEIYKDPETKEHFEFGDDIPEKPEREMTDQINRPIMLIKFPASLKSFYMQKCEDDKRLTESVDLLLPGVGEVVGGSMRMWNADELMEAYKKENMDPAPYYWFTDQRKYGSCPHGGYGLGLERFICALLGIHHIRDACLYPRYRGRISP 532
            GE   + LD F+AM E  RA +  +  KK L+LE  A+ K  K +E      AE   R+E A+++ I  DPSLP+A + KI  L       G ++  RV + GW H LR + KKL F++LRDGTG+ Q V++GN+CMT DA  L REA++++YG    DE  RA+G   G EL  DYWELVG+S A+I+M+ T +SNPDIL+NQRHLV+RG+KAS+++RMRSIIT CFREHFF   Y EL+PPTLV TQCEGGSTLF+F YF +  YLTQSSQLYLETGI AV DCFCILPSYRAEKS TRRHLAEFHHIEAE  FI FEDLL+ IE L+ DV+DRV +K  G++   L     KP   L+RPF R+TY DAV++CR H IYKD  T+ HFEFGDDIPE PER+MTD I  PI++IKFP +LKSFYM++C +D+ LTESVD+L+PGVGEVVGGSMRM + DELMEAY++EN+DPAPYYWF+DQRKYG+CPHGGYGLGLERFIC  L  HHIRD CLYPRYRGRI P
Sbjct:    9 GEMEALGLDAFMAMDEAARANLPKSRLKKMLRLEANAKKKEEKAKEQAGAQEAETRRRMEEAEKVVITMDPSLPQAVKCKIGKLKCAPAEDGGHIGTRVMLQGWTHRLRWESKKLLFLELRDGTGYIQAVMSGNLCMTKDALMLNREASVSLYGTVCSDE--RAKG---GMELHVDYWELVGSSPAEIEMILTHESNPDILLNQRHLVIRGTKASAVLRMRSIITQCFREHFFANDYVELAPPTLVQTQCEGGSTLFHFDYFGEPAYLTQSSQLYLETGIPAVGDCFCILPSYRAEKSSTRRHLAEFHHIEAEFAFIDFEDLLQRIEYLIADVVDRVIEK-GGDLLQVLNPGL-KP---LKRPFKRLTYTDAVKFCRQHNIYKDEATQTHFEFGDDIPEGPERKMTDMIGEPILMIKFPMTLKSFYMKRCPEDRALTESVDVLIPGVGEVVGGSMRMHDYDELMEAYERENIDPAPYYWFSDQRKYGTCPHGGYGLGLERFICYALAQHHIRDVCLYPRYRGRIEP 524          

BLAST of Gcaud2259.t1 vs. uniprot
Match: M2X2L0_GALSU (Asparagine--tRNA ligase n=2 Tax=Galdieria sulphuraria TaxID=130081 RepID=M2X2L0_GALSU)

HSP 1 Score: 640 bits (1650), Expect = 6.070e-225
Identity = 322/531 (60.64%), Postives = 390/531 (73.45%), Query Frame = 0

Query:    5 TEPGPTVEE---GEFHKMTLDTFVAMSEDDRAKMSATARKKWLKLEKKAQDKSRKEEEAXXXXAAENAARLERAKQIKIEQDPSLPEAKQVKIRDLGKYVDQRVKVYGWVHHLRADGKKLWFIDLRDGTGFAQVVLTGNMCMTYDAATLYREAAIAVYGKWIKDEQGRAQGSFPGFELAADYWELVGASCADIDMLFTKDSNPDILMNQRHLVLRGSKASSIMRMRSIITHCFREHFFDRGYTELSPPTLVNTQCEGGSTLFNFKYFEDQGYLTQSSQLYLETGIAAVNDCFCILPSYRAEKSQTRRHLAEFHHIEAERPFITFEDLLETIEDLVCDVIDRVGKKMEGEMDTFLRASAGKPIPKLERPFLRMTYEDAVQYCRDHEIYKDPETKEHFEFGDDIPEKPEREMTDQINRPIMLIKFPASLKSFYMQKCEDDKRLTESVDLLLPGVGEVVGGSMRMWNADELMEAYKKENMDPAPYYWFTDQRKYGSCPHGGYGLGLERFICALLGIHHIRDACLYPRYRGRISP 532
            TE   + EE    +  ++TLD F+ + E  +  +    RKK  +L K+ + K +K    XXXX                  DPSLP A  +KIRD+  YVD+RV V GWVHH+R DGKKL FI+LRDGTGF Q VL+  +C TY+A TL+REA + + G    DE  RA+GSFPG EL  DYW+L+G S ++I+ + T +SNPDIL+N RHLV+RG++ S+++++RSIIT CFREHFFDRGY E+ PPT+V TQCEGGSTLF   YF +  YLTQSSQ+YLET IA+V DCFCILPSYRAEKS TRRHLAEFHHIEAE PFI FEDLL  IEDLV DV+ RV   ME      L  +    +PK  RPF RMTYEDA++YC+++ IYKD  TK HFEFGDDIPE PER+MTDQIN PI L +FP SLKSFYM+KCED+ RLTESVDLLLPGVGE+VGGSMR+   +EL+EAY++E MDP+PYYWFTDQRKYGS PHGGYGLGLERF C LLG++H+RD CLYPRY+ R  P
Sbjct:    9 TEEKESEEEQLRNQLKEVTLDDFIKLDEQQKNLLPKARRKKLERLLKEQERKEQKAXXXXXXXXXXXXXXXXXXXXXXXXXDPSLPFATPIKIRDVPSYVDKRVTVQGWVHHIREDGKKLLFIELRDGTGFLQCVLSDLLCQTYEALTLHREATVLLKGTLTSDE--RAKGSFPGIELRVDYWQLIGPSPSEIENILTYESNPDILLNNRHLVIRGTRTSTVLKLRSIITQCFREHFFDRGYVEVFPPTIVQTQCEGGSTLFQLDYFGEPAYLTQSSQMYLETAIASVGDCFCILPSYRAEKSSTRRHLAEFHHIEAECPFINFEDLLFKIEDLVSDVMRRV---MEKASHLVLSLNPNIQVPK--RPFRRMTYEDAIRYCQENNIYKDEVTKMHFEFGDDIPELPERKMTDQINEPIFLTRFPVSLKSFYMKKCEDNSRLTESVDLLLPGVGEIVGGSMRIHELEELLEAYRREKMDPSPYYWFTDQRKYGSAPHGGYGLGLERFCCYLLGLYHVRDVCLYPRYKDRCKP 532          

BLAST of Gcaud2259.t1 vs. uniprot
Match: A0A1X6PH88_PORUM (Asparagine--tRNA ligase n=1 Tax=Porphyra umbilicalis TaxID=2786 RepID=A0A1X6PH88_PORUM)

HSP 1 Score: 635 bits (1637), Expect = 1.770e-222
Identity = 320/533 (60.04%), Postives = 386/533 (72.42%), Query Frame = 0

Query:   19 MTLDTFVAMSEDDRAKMSATARKKWLKLEKKAQDKSRKEEEAXXXXAAENAARLERAKQIKIEQDPSLPEAKQVKIRDLGKYV-------------------DQRVKVYGWVHHLRADGKKLWFIDLRDGTGFAQVVLTGNMCMTYDAATLYREAAIAVYGKWIKDEQGRAQGSFPGFELAADYWELVGASCADIDMLFTKDSNPDILMNQRHLVLRGSKASSIMRMRSIITHCFREHFFDRGYTELSPPTLVNTQCEGGSTLFNFKYFEDQGYLTQSSQLYLETGIAAVNDCFCILPSYRAEKSQTRRHLAEFHHIEAERPFITFEDLLETIEDLVCDVIDRVGKKMEGEMDTFLRASAGKPIPKLERPFLRMTYEDAVQYCRDHEIYKDPETKEHFEFGDDIPEKPEREMTDQINRPIMLIKFPASLKSFYMQKCEDDKRLTESVDLLLPGVGEVVGGSMRMWNADELMEAYKKENMDPAPYYWFTDQRKYGSCPHGGYGLGLERFICALLGIHHIRDACLYPRYRGRISP 532
            +TL+ F+AM    RA +     KK LK E+    K  K  E       E+  R ERA  + +E D SLP  + +KIRDL                         RVKV GWVH +R +G KL F++LRDGTGF Q VLTG  C T DA  L+RE+++ ++G    D   RA+G   G EL +DYWELVG S A+I+ + + DSNPDIL+  RHLV+RG++ASSI+RMRSIIT CFREH FDRGY EL+PPTLVNT CEGGSTLF+  YF +  YLTQSSQ+YLETGI AV DCFC+LPSYRAEKS TRRHLAEFHH+EAE PFITFE+LL+ +EDLV DV++RV K+        L AS    +  ++RPF RMTY DA+ YCRD+ IYKD ET+ HF+FGDDIPE PER+MTD+I  PI+L +FPA+LKSFYMQKC+DD+ +TESVDLL+PGVGEVVGGSMRMW  +EL  AYK+E MDP PYYW+TDQRKYG+CPHGGYGLGLERF+C +LGIHHIRDACL+PRYRGRI P
Sbjct:   42 VTLEQFLAMDATARAALPKARLKKMLKTEQGHLKKVAKAAEMAARAEEESRVRAERAAGVVLEVDASLPAPRTIKIRDLDAPAVADGDAGXXXXXAAAHPLPGTRVKVSGWVHRIRWEGAKLLFLELRDGTGFLQAVLTGTQCQTTDAINLHRESSVTLWGTLSAD--ARAKG---GVELRSDYWELVGRSPAEIEEVLSHDSNPDILLRNRHLVVRGTRASSILRMRSIITQCFREHLFDRGYIELTPPTLVNTMCEGGSTLFHLDYFGEPAYLTQSSQMYLETGIPAVGDCFCLLPSYRAEKSSTRRHLAEFHHLEAECPFITFEELLDRVEDLVVDVVERVMKRGGS-----LLASVNPNLAAMKRPFKRMTYRDALVYCRDNLIYKDEETQTHFDFGDDIPEGPERKMTDKIGEPILLHRFPAALKSFYMQKCDDDREVTESVDLLVPGVGEVVGGSMRMWKEEELAAAYKREGMDPKPYYWYTDQRKYGTCPHGGYGLGLERFVCFVLGIHHIRDACLFPRYRGRIEP 564          

BLAST of Gcaud2259.t1 vs. uniprot
Match: M1UT91_CYAM1 (Asparagine--tRNA ligase n=1 Tax=Cyanidioschyzon merolae (strain 10D) TaxID=280699 RepID=M1UT91_CYAM1)

HSP 1 Score: 629 bits (1621), Expect = 9.320e-221
Identity = 313/526 (59.51%), Postives = 386/526 (73.38%), Query Frame = 0

Query:   11 VEEGEFHKMTLDTFVAMSEDDRAKMSATARKKWLKLEKKAQDKSRKEEEAXXXXAAENAARLERAKQIKIEQDPSLPEAKQVKIRDL----GKYVDQRVKVYGWVHHLRADGKKLWFIDLRDGTGFAQVVLTGNMCMTYDAATLYREAAIAVYGKWIKDEQGRAQGSFPGFELAADYWELVGASCADIDMLFTKDSNPDILMNQRHLVLRGSKASSIMRMRSIITHCFREHFFDRGYTELSPPTLVNTQCEGGSTLFNFKYFEDQGYLTQSSQLYLETGIAAVNDCFCILPSYRAEKSQTRRHLAEFHHIEAERPFITFEDLLETIEDLVCDVIDRVGKKMEGEMDTFLRASAGKPIPKLERPFLRMTYEDAVQYCRDHEIYKDPETKEHFEFGDDIPEKPEREMTDQINRPIMLIKFPASLKSFYMQKCEDDKRLTESVDLLLPGVGEVVGGSMRMWNADELMEAYKKENMDPAPYYWFTDQRKYGSCPHGGYGLGLERFICALLGIHHIRDACLYPRYRGRISP 532
            +E+G  + ++LD ++ MSE++R ++  T  KK  +    A+ K+ +  E     A E   RLE A+Q++IE D SLP+    K+RD+     K+VDQRV + GWVHH R DGK+L F++LRDGTGF Q VL+  +CMTYDA TL REA++ V+G    D   RA+G   G EL AD+WELV  S  +I+M+ T +SNPDIL+N RHLV+RG + S+ +R+RS+IT CFREH+F RGY EL+PPT+VNT CEGGSTLF   YF    YLTQSSQ+YLE+GI AV DCFC+LPSYRAE S TRRHLAEFHHIEAE PFITF DLL+ IEDLVCDVI RV  K  G++   L      P     RPF R+TY DA+ YCR+HEIYKD E K HFE+GDDIPE+PER MTDQI  PI L  FP S+K+FYMQ C D   LTESVDLL+PGVGE+VGGSMR+W   +LM+AY +E +DP PYYW+TDQRK+GSCPHGGYGLGLERF C +LG+ H+R+ CLYPRYRGRISP
Sbjct:    4 IEQGTSN-ISLDDYLKMSEEERQQLPKTRLKKLQRQAALAERKAARAAELAANAATEQRERLEEARQVRIELDSSLPKPLICKLRDIFASPEKFVDQRVCLAGWVHHWRMDGKRLAFLELRDGTGFMQCVLSNELCMTYDALTLCREASVRVWGTVRHD--ARAKG---GLELHADFWELVAPSTPEIEMVLTHESNPDILLNNRHLVIRGRRTSTTLRLRSLITQCFREHYFQRGYVELAPPTIVNTMCEGGSTLFKLDYFGINAYLTQSSQMYLESGIPAVGDCFCVLPSYRAELSDTRRHLAEFHHIEAEMPFITFADLLDAIEDLVCDVIARVLAKA-GDLVRDLNPELVVP----RRPFRRLTYADAIAYCREHEIYKDEEQKIHFEYGDDIPERPERLMTDQIGEPIFLTHFPVSMKAFYMQPCTDQPDLTESVDLLMPGVGEIVGGSMRIWRYKQLMDAYAREQIDPKPYYWYTDQRKFGSCPHGGYGLGLERFCCYVLGVRHVREVCLYPRYRGRISP 518          

BLAST of Gcaud2259.t1 vs. uniprot
Match: A0A7S1XBT5_9RHOD (Asparagine--tRNA ligase n=1 Tax=Compsopogon caeruleus TaxID=31354 RepID=A0A7S1XBT5_9RHOD)

HSP 1 Score: 615 bits (1586), Expect = 2.130e-215
Identity = 312/519 (60.12%), Postives = 387/519 (74.57%), Query Frame = 0

Query:   19 MTLDTFVAMSEDDRAKMSATARKKWLKLEKKAQDKSRKEEEAXXXXAAENAARLERAKQIKIEQDPSLPEAKQ-VKIRDL-GKYVD---QRVKVYGWVHHLRADGKKLWFIDLRDGTGFAQVVLTGNMCMTYDAATLYREAAIAVYGKWIKDEQGRAQGSFPGFELAADYWELVGASCADIDMLFTKDSNPDILMNQRHLVLRGSKASSIMRMRSIITHCFREHFFDRGYTELSPPTLVNTQCEGGSTLFNFKYFEDQGYLTQSSQLYLETGIAAVNDCFCILPSYRAEKSQTRRHLAEFHHIEAERPFITFEDLLETIEDLVCDVIDRVGKKMEGEMDTFLRASAGKPIPKLERPFLRMTYEDAVQYCRDHEIYKDPETKEHFEFGDDIPEKPEREMTDQINRPIMLIKFPASLKSFYMQKCEDDKRLTESVDLLLPGVGEVVGGSMRMWNADELMEAYKKENMDPAPYYWFTDQRKYGSCPHGGYGLGLERFICALLGIHHIRDACLYPRYRGRISP 532
            ++L+ F+ MSE+DRAK+  + +KK  KLE   + K     + XXXX           +++KI  D SLP A++ +KIRDL G+ +D    RVK+ GWVH LR +G+KL F++LRDGTGF   VL+  +CMT +A TL+REAA+A++G  +KDE  RA+G   G EL  DYWELVG+S  +I+ +   DS PD+LMN R+LV+RG++ASS++RMRSIIT  FREHFF RGY EL PPTLVNT CEGGSTLF   YF +  YLTQSSQ+YLETGI AV DCFCILPSYRAEKSQTRRHLAEFHHIEAE PFI FE LL  IEDLVCDV +R  +K +  +D          +   +RPF RM Y DA+ +CR++ IYKD ETK HFE+GDDIPE PER+MTDQI  PI+L +FPAS+KSFYMQKCEDDKRLTESVDLL+PGVGEVVGGSMR ++ + LME+Y++E + P  Y W++D RK+G+CPHGGYGLGLERF C L+ IHHIRD CLYPR  GR++P
Sbjct:   13 VSLEEFLVMSEEDRAKLPKSRQKKLAKLEASMKRKDEVANQXXXXXXXXXXXXXXXXRKVKITMDLSLPSAQEGMKIRDLHGRGIDIVGVRVKISGWVHQLRWEGRKLMFVELRDGTGFLPAVLSNELCMTVEAITLHREAAVALWGTVVKDE--RAKG---GIELQCDYWELVGSSPGEIEFILKPDSGPDVLMNYRYLVIRGTRASSVLRMRSIITQSFREHFFARGYVELFPPTLVNTSCEGGSTLFKLDYFGEDAYLTQSSQMYLETGIPAVGDCFCILPSYRAEKSQTRRHLAEFHHIEAECPFINFEGLLSRIEDLVCDVTERAMEKGKDLLDVL-----NPDLKPFKRPFRRMNYSDAIDFCRENNIYKDEETKTHFEYGDDIPEGPERKMTDQIGEPILLCRFPASMKSFYMQKCEDDKRLTESVDLLVPGVGEVVGGSMRTFDYEALMESYEREGVPPENYVWYSDMRKFGTCPHGGYGLGLERFCCYLMNIHHIRDVCLYPRMIGRLNP 521          

BLAST of Gcaud2259.t1 vs. uniprot
Match: A0A7J7IJN4_9RHOD (Asparagine--tRNA ligase n=1 Tax=Cyanidiococcus yangmingshanensis TaxID=2690220 RepID=A0A7J7IJN4_9RHOD)

HSP 1 Score: 606 bits (1563), Expect = 6.020e-213
Identity = 290/454 (63.88%), Postives = 345/454 (75.99%), Query Frame = 0

Query:   83 DPSLPEAKQVKIRDL----GKYVDQRVKVYGWVHHLRADGKKLWFIDLRDGTGFAQVVLTGNMCMTYDAATLYREAAIAVYGKWIKDEQGRAQGSFPGFELAADYWELVGASCADIDMLFTKDSNPDILMNQRHLVLRGSKASSIMRMRSIITHCFREHFFDRGYTELSPPTLVNTQCEGGSTLFNFKYFEDQGYLTQSSQLYLETGIAAVNDCFCILPSYRAEKSQTRRHLAEFHHIEAERPFITFEDLLETIEDLVCDVIDRVGKKMEGEMDTFLRASAGKPIPKLERPFLRMTYEDAVQYCRDHEIYKDPETKEHFEFGDDIPEKPEREMTDQINRPIMLIKFPASLKSFYMQKCEDDKRLTESVDLLLPGVGEVVGGSMRMWNADELMEAYKKENMDPAPYYWFTDQRKYGSCPHGGYGLGLERFICALLGIHHIRDACLYPRYRGRISP 532
            DP+LP+    KIRD+       + +RV + GWVHH R DGK+L F+DLRDGTGF Q VLT  +CMTYDA TL REA++ V+G   +D + ++     G EL ADYWELV  S  +I+M  T +SNPDIL+N RHLV+RG ++S I+R+RS+IT CFREH+FDRGY EL+PPT+VNT CEGGSTLF   YF    YLTQSSQ+YLET I AV DCFCILPSYRAEKS TRRHLAEFHHIEAE PFI FE+LL  IEDLVCDVI R+  K  G++   L  +   P    +RPF RMTY DA+ YCR+H+IYKD E K HFEFGDDIPE+PER MTDQI  PI+L  FP SLK+FYMQ C D   LTESVD+LLPGVGE++GGSMRMW  +ELM+AY +E MDP  YYW+TDQRK+GSCPHGGYGLGLERF C +L + H+R+ CLYPRYRGR+SP
Sbjct:   11 DPNLPKPLVCKIRDIFADPDTMLQRRVCLAGWVHHWRMDGKRLAFMDLRDGTGFLQCVLTNELCMTYDALTLCREASVRVWGSLQRDARAKS-----GLELQADYWELVAPSTPEIEMALTHESNPDILLNHRHLVIRGRQSSMILRLRSLITQCFREHYFDRGYVELAPPTIVNTMCEGGSTLFKLDYFGSDAYLTQSSQMYLETAIPAVGDCFCILPSYRAEKSSTRRHLAEFHHIEAEMPFINFENLLNAIEDLVCDVISRLLDKA-GDLVRELNPAVAVP----QRPFRRMTYADAIAYCREHDIYKDEENKVHFEFGDDIPERPERLMTDQIGEPILLTHFPVSLKAFYMQPCVDQPDLTESVDVLLPGVGEIIGGSMRMWRYEELMQAYAREMMDPQSYYWYTDQRKFGSCPHGGYGLGLERFCCYVLDVKHVREVCLYPRYRGRLSP 454          

BLAST of Gcaud2259.t1 vs. uniprot
Match: A0A3B3QUQ6_9TELE (Asparagine--tRNA ligase n=1 Tax=Paramormyrops kingsleyae TaxID=1676925 RepID=A0A3B3QUQ6_9TELE)

HSP 1 Score: 560 bits (1444), Expect = 2.730e-193
Identity = 276/494 (55.87%), Postives = 354/494 (71.66%), Query Frame = 0

Query:   40 RKKWLKLEKKAQDKSRKEEEAXXXXAAENAARLERAKQIKIEQDPSLPEAKQVKIRDLGKYVDQRVKVYGWVHHLRADGKKLWFIDLRDGTGFAQVVLTGNMCMTYDAATLYREAAIAVYGKWIKDEQGRAQGSFPGFELAADYWELVG-ASCADIDMLFTKDSNPDILMNQRHLVLRGSKASSIMRMRSIITHCFREHFFDRGYTELSPPTLVNTQCEGGSTLFNFKYFEDQGYLTQSSQLYLETGIAAVNDCFCILPSYRAEKSQTRRHLAEFHHIEAERPFITFEDLLETIEDLVCDVIDRVGKKMEGEMDTFLRASAGKPIPKLERPFLRMTYEDAVQYCRDHEIYKDPETKEHFEFGDDIPEKPEREMTDQINRPIMLIKFPASLKSFYMQKCEDDKRLTESVDLLLPGVGEVVGGSMRMWNADELMEAYKKENMDPAPYYWFTDQRKYGSCPHGGYGLGLERFICALLGIHHIRDACLYPRYRGRISP 532
            +K+W K + K   K +KE E     A      LE AK+I I +D SLPE K+VKI  LG+Y +QRVK++GWVH LR  GK L FI LRDGTGF Q VLT  +C  YDA  L  E+ + +YG+ +   +G+      G EL  D+WELVG A     D L  ++S+ D+ +N RH+++RG   S I+R RS++T CFR+HFF RGY E++PPTLV TQ EGGSTLF   YF +Q YLTQSSQLYLET I A+ DCFCI  SYRAE+S+TRRHLAE+ H+EAE PF+TF DLL+ +EDLVCDV+DRV K   G++ + +      P    +RPF RM Y +A+ + ++H+I KD  +  ++EFG+DIPE PER+MTD IN PI+L +FPA +KSFYMQ+C +D+RLTESVD+L+P VGE+VGGSMR+W+ADEL+E YK+E +DP PYYW+TDQRKYG+CPHGGYGLGLERF+  LL   HIRD CLYPR+  R  P
Sbjct:   78 KKQWNKEQMKNDSKLKKETE----DADRREKNLEEAKKIFIVKDSSLPEPKKVKIHQLGEYREQRVKLFGWVHRLRRQGKNLMFIVLRDGTGFLQCVLTDQLCQCYDAVVLSTESTVGLYGRVMPVPEGKEAPG--GHELICDFWELVGLAPPGGADNLLNEESDVDVQLNNRHMMIRGENMSKILRARSVVTQCFRDHFFSRGYYEVTPPTLVQTQVEGGSTLFGLNYFGEQAYLTQSSQLYLETCIPALGDCFCIAQSYRAEQSRTRRHLAEYTHVEAECPFLTFNDLLDRLEDLVCDVVDRVLKSPMGDLISEINPDFKPP----KRPFKRMDYTEAIAWLKEHDIRKDDGS--YYEFGEDIPEAPERKMTDAINEPILLCRFPAEIKSFYMQRCPEDRRLTESVDVLMPNVGEIVGGSMRIWDADELLEGYKREGIDPTPYYWYTDQRKYGTCPHGGYGLGLERFLTWLLNRFHIRDVCLYPRFIQRCRP 559          

BLAST of Gcaud2259.t1 vs. uniprot
Match: A0A7R8WFV0_9CRUS (Asparagine--tRNA ligase n=1 Tax=Cyprideis torosa TaxID=163714 RepID=A0A7R8WFV0_9CRUS)

HSP 1 Score: 557 bits (1436), Expect = 5.280e-192
Identity = 269/472 (56.99%), Postives = 350/472 (74.15%), Query Frame = 0

Query:   66 AENAAR----LERAKQIKIEQDPSLPEAKQVKIRDLGKYVDQRVKVYGWVHHLRADGKKLWFIDLRDGTGFAQVVLTGNMCMTYDAATLYREAAIAVYGKWIKDEQGRAQGSFPGFELAADYWELVGASC-ADIDMLFTKDSNPDILMNQRHLVLRGSKASSIMRMRSIITHCFREHFFDRGYTELSPPTLVNTQCEGGSTLFNFKYFEDQGYLTQSSQLYLETGIAAVNDCFCILPSYRAEKSQTRRHLAEFHHIEAERPFITFEDLLETIEDLVCDVIDRVGKKMEGEMDTFLRASAGKPIPKLERPFLRMTYEDAVQYCRDHEIYKDPETKEHFEFGDDIPEKPEREMTDQINRPIMLIKFPASLKSFYMQKCEDDKRLTESVDLLLPGVGEVVGGSMRMWNADELMEAYKKENMDPAPYYWFTDQRKYGSCPHGGYGLGLERFICALLGIHHIRDACLYPRYRGRISP 532
            AE+A R    LE AKQ+KIE+D +LP A+++KIR+L  + D+R+KVYGWVH +R  GK L FI LRDGTG+ Q VL   +C T +A TL  EAA+A+YG+     +G+      G ELAADYWELVG S     + +  ++++PD++++ RH+++RG   S +++MRS+I+  FR H++DRGY E++PPTLV TQ EGGSTLF   YF D  YLTQSSQLYLET I AV DCF I  SYRAE S+TRRHLAE+ HIE E PFI FE+LL+ IEDL+CDV+ RV +   G +   L      P    +RPFLRM Y+DA+QY ++H+I K+  T   +EFGDDIPE PER+MTD+IN+PIML +FPA +K+FYM +C++D R+TESVD+L+PGVGE++GGSMR WN DELM+ +++E +DP+PYYW+TDQRK+GSCPHGGYGLGLERF C LL  HHIRD CLYPR+  R  P
Sbjct:  101 AEDAERRLKNLELAKQVKIEEDLTLPPAEKLKIRELKNWRDKRIKVYGWVHRIRRQGKALIFITLRDGTGYLQCVLNDKLCQTVEAVTLSTEAAVAIYGQLTAVPEGKTAPG--GHELAADYWELVGPSPPGGAESVLNEEAHPDVMLDNRHMLIRGENTSKVLKMRSVISQAFRSHYYDRGYFEITPPTLVQTQVEGGSTLFKLDYFGDNAYLTQSSQLYLETAIPAVGDCFTITQSYRAEASRTRRHLAEYMHIEGECPFIDFEELLDRIEDLICDVVKRVLEHPLGHLVQELNPGFIPP----KRPFLRMDYKDAIQYLKEHDIRKEDGT--FYEFGDDIPEAPERKMTDEINQPIMLCRFPAEIKAFYMPRCKEDNRVTESVDVLMPGVGEILGGSMRTWNYDELMKGFQREALDPSPYYWYTDQRKFGSCPHGGYGLGLERFACWLLNRHHIRDVCLYPRFIERCKP 564