Gcaud2020.t1 (mRNA) Gracilaria caudata M_176_S67 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview


Name	Gcaud2020.t1
Unique Name	Gcaud2020.t1
Type	mRNA
Organism	Gracilaria caudata M_176_S67 male (Gracilaria caudata M_176_S67 male)
Sequence length	756

Alignments

The following features are aligned


Aligned Feature	Feature Type	Alignment Location
NODE_1148_length_13309_cov_4.323498	contig	NODE_1148_length_13309_cov_4.323498:7769..10297 +

Analyses

This mRNA is derived from or has results from the following analyses


Analysis Name	Date Performed
Gracilaria caudata M_176_S67 male OGS1.0	2022-05-09

Properties


Property Name	Value
Seed ortholog	10224.XP_002734747.1
PFAMs	GRDP-like
Max annot lvl	33208\|Metazoa
Evalue	1e-50
EggNOG OGs	28JC1@1\|root,2QRQZ@2759\|Eukaryota,38E6R@33154\|Opisthokonta,3BHPZ@33208\|Metazoa,3D58M@33213\|Bilateria
Description	Glycine-rich domain-containing protein-like
COG category	S

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Species	Type	Position
Gcaud2020	Gcaud2020	Gracilaria caudata M_176_S67 male	gene	NODE_1148_length_13309_cov_4.323498 7769..10297 +

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Species	Type	Position
Gcaud2020.t1	Gcaud2020.t1	Gracilaria caudata M_176_S67 male	polypeptide	NODE_1148_length_13309_cov_4.323498 7769..10297 +

The following start_codon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Species	Type	Position
Gcaud2020.t1.start1	Gcaud2020.t1.start1	Gracilaria caudata M_176_S67 male	start_codon	NODE_1148_length_13309_cov_4.323498 7769..7771 +

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Species	Type	Position
Gcaud2020.t1.CDS1	Gcaud2020.t1.CDS1	Gracilaria caudata M_176_S67 male	CDS	NODE_1148_length_13309_cov_4.323498 7769..9890 +
Gcaud2020.t1.CDS2	Gcaud2020.t1.CDS2	Gracilaria caudata M_176_S67 male	CDS	NODE_1148_length_13309_cov_4.323498 10152..10297 +

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Species	Type	Position
Gcaud2020.t1.exon1	Gcaud2020.t1.exon1	Gracilaria caudata M_176_S67 male	exon	NODE_1148_length_13309_cov_4.323498 7769..9890 +
Gcaud2020.t1.exon2	Gcaud2020.t1.exon2	Gracilaria caudata M_176_S67 male	exon	NODE_1148_length_13309_cov_4.323498 10152..10297 +

The following intron feature(s) are a part of this mRNA:


Feature Name	Unique Name	Species	Type	Position
Gcaud2020.t1.intron1	Gcaud2020.t1.intron1	Gracilaria caudata M_176_S67 male	intron	NODE_1148_length_13309_cov_4.323498 9891..10151 +

The following stop_codon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Species	Type	Position
Gcaud2020.t1.stop1	Gcaud2020.t1.stop1	Gracilaria caudata M_176_S67 male	stop_codon	NODE_1148_length_13309_cov_4.323498 10295..10297 +

Sequences

The following sequences are available for this feature:

mRNA sequence
mRNA - spliced transcript sequence
protein sequence
mRNA from alignment at NODE_1148_length_13309_cov_4.323498:7769..10297+
coding sequence from alignment at NODE_1148_length_13309_cov_4.323498:7769..10297+

mRNA sequence

>Gcaud2020.t1 ID=Gcaud2020.t1|Name=Gcaud2020.t1|organism=Gracilaria caudata M_176_S67 male|type=mRNA|length=756bp
MNLLAFNLDGRKSKAKRGLASSVKALVSVLNEVDEYPALYFNHLVLREAI
RRYEHLWIPLLANAKPKQRLRLVPPLDVEWVWFCHMLCPAKYATDCAAMW
QAVRPNEPDFILDHVLLGPKKRLAGKLEAEQLWRQMFPHEPFDNTDAIKD
APMQVPMKRGSASEASRISYNIVAAAERQMVFQYQVGIMPQYEENSFLRS
AVRRYKKFLKLQQMQPDKIWVPTYDIDVCWHAHMMHPRWYHSNTVKLKGS
VLKHDDTLNDRATGSELNQRWNATASTWKQLFSSPVHFAGGMWRGNVETS
ERVQRPALVESIVALQADCASPANAEYKVWDADMSALDLSAGLDWVHMTE
WRRKNEPADRSSPCSTQNKVERFTCLGQETGDAGILLSRTIHFLMYKNDG
SNAEPTRFASVVEVFGAKPSSEKPGYPLATAHHVLPNSICGWESSQVPAR
RCAASATETVFVLRVGGEDFGLIAGEWYGFEPPRKGVPGIPGSRGGREWG
VPGIPGTRGDPGHLNLRLWLLGAKTRRRWINLRPVGQKKQPLSYSLELPS
LSVAGGIAAGEVGFSLQNATVRVSDRSQLLIAYLISTAAAALFTTLQPRF
IPRGGAIVDSHETMYPAWSVQQKDYATVRGAGGEVPTEQGNQGLGMMLLA
LQKSAWDCLDFPGSPKREFRLVRAKADAKAKEGPIEHSPMFGGIVDEIGS
PEGTDGCGQFVWLRYVIARAGEKLIETGYGQVVNLAELELSDVLKPAQFL
VSQLA*

spliced messenger RNA

>Gcaud2020.t1 ID=Gcaud2020.t1|Name=Gcaud2020.t1|organism=Gracilaria caudata M_176_S67 male|type=mRNA|length=2268bp|location=Sequence derived from alignment at NODE_1148_length_13309_cov_4.323498:7769..10297+ (Gracilaria caudata M_176_S67 male)|Notes=Excludes all bases but those of type(s): exon.  
ATGAACCTCCTGGCTTTTAACCTGGATGGACGCAAATCGAAGGCAAAGCG
AGGTCTGGCGTCGTCTGTGAAGGCGTTAGTGTCTGTTCTCAATGAAGTCG
ATGAGTACCCGGCTTTGTATTTCAACCACCTCGTGTTGCGGGAGGCCATC
CGCCGCTATGAACATTTATGGATTCCCCTTCTTGCCAATGCCAAGCCCAA
GCAACGCCTGCGTCTCGTTCCGCCGCTTGACGTTGAATGGGTTTGGTTCT
GCCACATGCTCTGTCCGGCCAAATACGCCACTGACTGTGCCGCCATGTGG
CAGGCCGTGCGCCCTAACGAGCCTGATTTCATTTTAGACCATGTGCTTCT
GGGGCCGAAGAAGAGACTGGCCGGAAAATTGGAGGCTGAACAATTGTGGC
GACAGATGTTTCCACACGAGCCTTTCGACAACACGGATGCAATCAAGGAC
GCACCCATGCAAGTGCCCATGAAGCGTGGCTCCGCCTCAGAAGCGTCCCG
CATCTCGTACAATATCGTGGCCGCAGCGGAAAGGCAGATGGTGTTCCAGT
ATCAGGTCGGCATCATGCCGCAATATGAAGAGAACAGCTTTCTGCGGTCC
GCTGTCCGCCGATACAAAAAGTTTCTCAAACTGCAACAGATGCAACCGGA
TAAGATTTGGGTTCCAACATATGATATCGACGTGTGCTGGCACGCTCATA
TGATGCACCCACGTTGGTATCATAGCAACACCGTGAAATTGAAGGGATCA
GTACTGAAACATGATGACACGCTCAACGATAGAGCAACGGGATCCGAGCT
CAACCAGAGGTGGAATGCGACCGCGAGTACATGGAAACAGCTTTTCTCTT
CTCCAGTGCACTTTGCAGGTGGAATGTGGCGGGGAAACGTTGAAACCTCG
GAAAGAGTACAACGACCGGCTCTCGTTGAGAGCATTGTTGCTCTGCAGGC
GGATTGTGCTTCACCAGCAAATGCCGAGTACAAAGTGTGGGATGCTGACA
TGAGCGCATTGGATCTAAGCGCCGGCTTGGACTGGGTTCACATGACAGAG
TGGCGTAGGAAGAATGAGCCTGCGGATCGTAGCAGCCCATGCAGTACCCA
AAACAAAGTGGAACGCTTCACCTGCCTCGGACAAGAAACAGGAGATGCAG
GTATTCTCTTGAGTCGAACGATACATTTTCTGATGTACAAGAATGACGGC
TCCAATGCTGAACCAACCCGTTTTGCCTCTGTAGTGGAGGTGTTCGGAGC
GAAGCCATCTTCAGAGAAACCAGGATACCCGCTCGCAACAGCGCACCATG
TGCTTCCCAACAGTATATGCGGTTGGGAATCGTCCCAAGTCCCGGCTCGC
AGATGTGCTGCATCCGCCACAGAAACTGTTTTTGTTTTGAGGGTTGGCGG
AGAGGACTTTGGCCTCATTGCAGGAGAATGGTATGGTTTTGAACCTCCTC
GGAAAGGGGTTCCTGGAATTCCAGGTAGCAGGGGTGGAAGAGAATGGGGG
GTGCCTGGTATTCCAGGAACGAGGGGAGATCCGGGGCATCTGAATCTGCG
CTTATGGTTACTTGGTGCGAAAACTCGAAGGAGATGGATCAACTTGAGGC
CCGTAGGCCAGAAAAAGCAGCCTCTGTCTTATTCGCTTGAGCTTCCCTCC
CTCTCTGTAGCAGGAGGCATCGCAGCAGGTGAAGTGGGCTTCAGTCTCCA
AAATGCAACAGTGCGTGTGAGTGATAGGAGCCAGCTGCTGATAGCATATC
TGATAAGCACAGCAGCAGCAGCGCTGTTCACAACACTGCAGCCGAGGTTC
ATTCCTCGTGGCGGTGCGATTGTTGATAGCCATGAAACCATGTATCCCGC
TTGGTCAGTGCAGCAGAAAGATTATGCCACGGTTCGGGGAGCCGGTGGGG
AAGTACCCACCGAGCAGGGTAACCAAGGCTTGGGAATGATGCTTCTCGCA
TTGCAGAAAAGCGCGTGGGACTGTCTCGACTTCCCAGGGTCTCCGAAGCG
GGAGTTCAGGCTCGTCAGGGCTAAAGCGGATGCAAAAGCGAAGGAAGGCC
CAATTGAGCACAGTCCCATGTTCGGTGGTATTGTGGATGAGATCGGCAGT
CCTGAGGGGACTGATGGCTGTGGGCAGTTTGTGTGGCTGAGGTATGTGAT
TGCGCGTGCTGGAGAGAAGCTTATTGAGACCGGATACGGGCAGGTGGTGA
ACCTTGCGGAGCTGGAGTTGTCGGACGTGCTGAAGCCTGCCCAATTCCTA
GTCTCACAGTTGGCGTAG

protein sequence of Gcaud2020.t1

>Gcaud2020.t1 ID=Gcaud2020.t1|Name=Gcaud2020.t1|organism=Gracilaria caudata M_176_S67 male|type=polypeptide|length=756bp
MNLLAFNLDGRKSKAKRGLASSVKALVSVLNEVDEYPALYFNHLVLREAI
RRYEHLWIPLLANAKPKQRLRLVPPLDVEWVWFCHMLCPAKYATDCAAMW
QAVRPNEPDFILDHVLLGPKKRLAGKLEAEQLWRQMFPHEPFDNTDAIKD
APMQVPMKRGSASEASRISYNIVAAAERQMVFQYQVGIMPQYEENSFLRS
AVRRYKKFLKLQQMQPDKIWVPTYDIDVCWHAHMMHPRWYHSNTVKLKGS
VLKHDDTLNDRATGSELNQRWNATASTWKQLFSSPVHFAGGMWRGNVETS
ERVQRPALVESIVALQADCASPANAEYKVWDADMSALDLSAGLDWVHMTE
WRRKNEPADRSSPCSTQNKVERFTCLGQETGDAGILLSRTIHFLMYKNDG
SNAEPTRFASVVEVFGAKPSSEKPGYPLATAHHVLPNSICGWESSQVPAR
RCAASATETVFVLRVGGEDFGLIAGEWYGFEPPRKGVPGIPGSRGGREWG
VPGIPGTRGDPGHLNLRLWLLGAKTRRRWINLRPVGQKKQPLSYSLELPS
LSVAGGIAAGEVGFSLQNATVRVSDRSQLLIAYLISTAAAALFTTLQPRF
IPRGGAIVDSHETMYPAWSVQQKDYATVRGAGGEVPTEQGNQGLGMMLLA
LQKSAWDCLDFPGSPKREFRLVRAKADAKAKEGPIEHSPMFGGIVDEIGS
PEGTDGCGQFVWLRYVIARAGEKLIETGYGQVVNLAELELSDVLKPAQFL
VSQLA*

mRNA from alignment at NODE_1148_length_13309_cov_4.323498:7769..10297+

Legend: polypeptidestart_codonCDSexonintronstop_codon

Hold the cursor over a type above to highlight its positions in the sequence below.

>Gcaud2020.t1 ID=Gcaud2020.t1|Name=Gcaud2020.t1|organism=Gracilaria caudata M_176_S67 male|type=mRNA|length=2529bp|location=Sequence derived from alignment at NODE_1148_length_13309_cov_4.323498:7769..10297+ (Gracilaria caudata M_176_S67 male)
ATGAACCTCCTGGCTTTTAACCTGGATGGACGCAAATCGAAGGCAAAGCG
AGGTCTGGCGTCGTCTGTGAAGGCGTTAGTGTCTGTTCTCAATGAAGTCG
ATGAGTACCCGGCTTTGTATTTCAACCACCTCGTGTTGCGGGAGGCCATC
CGCCGCTATGAACATTTATGGATTCCCCTTCTTGCCAATGCCAAGCCCAA
GCAACGCCTGCGTCTCGTTCCGCCGCTTGACGTTGAATGGGTTTGGTTCT
GCCACATGCTCTGTCCGGCCAAATACGCCACTGACTGTGCCGCCATGTGG
CAGGCCGTGCGCCCTAACGAGCCTGATTTCATTTTAGACCATGTGCTTCT
GGGGCCGAAGAAGAGACTGGCCGGAAAATTGGAGGCTGAACAATTGTGGC
GACAGATGTTTCCACACGAGCCTTTCGACAACACGGATGCAATCAAGGAC
GCACCCATGCAAGTGCCCATGAAGCGTGGCTCCGCCTCAGAAGCGTCCCG
CATCTCGTACAATATCGTGGCCGCAGCGGAAAGGCAGATGGTGTTCCAGT
ATCAGGTCGGCATCATGCCGCAATATGAAGAGAACAGCTTTCTGCGGTCC
GCTGTCCGCCGATACAAAAAGTTTCTCAAACTGCAACAGATGCAACCGGA
TAAGATTTGGGTTCCAACATATGATATCGACGTGTGCTGGCACGCTCATA
TGATGCACCCACGTTGGTATCATAGCAACACCGTGAAATTGAAGGGATCA
GTACTGAAACATGATGACACGCTCAACGATAGAGCAACGGGATCCGAGCT
CAACCAGAGGTGGAATGCGACCGCGAGTACATGGAAACAGCTTTTCTCTT
CTCCAGTGCACTTTGCAGGTGGAATGTGGCGGGGAAACGTTGAAACCTCG
GAAAGAGTACAACGACCGGCTCTCGTTGAGAGCATTGTTGCTCTGCAGGC
GGATTGTGCTTCACCAGCAAATGCCGAGTACAAAGTGTGGGATGCTGACA
TGAGCGCATTGGATCTAAGCGCCGGCTTGGACTGGGTTCACATGACAGAG
TGGCGTAGGAAGAATGAGCCTGCGGATCGTAGCAGCCCATGCAGTACCCA
AAACAAAGTGGAACGCTTCACCTGCCTCGGACAAGAAACAGGAGATGCAG
GTATTCTCTTGAGTCGAACGATACATTTTCTGATGTACAAGAATGACGGC
TCCAATGCTGAACCAACCCGTTTTGCCTCTGTAGTGGAGGTGTTCGGAGC
GAAGCCATCTTCAGAGAAACCAGGATACCCGCTCGCAACAGCGCACCATG
TGCTTCCCAACAGTATATGCGGTTGGGAATCGTCCCAAGTCCCGGCTCGC
AGATGTGCTGCATCCGCCACAGAAACTGTTTTTGTTTTGAGGGTTGGCGG
AGAGGACTTTGGCCTCATTGCAGGAGAATGGTATGGTTTTGAACCTCCTC
GGAAAGGGGTTCCTGGAATTCCAGGTAGCAGGGGTGGAAGAGAATGGGGG
GTGCCTGGTATTCCAGGAACGAGGGGAGATCCGGGGCATCTGAATCTGCG
CTTATGGTTACTTGGTGCGAAAACTCGAAGGAGATGGATCAACTTGAGGC
CCGTAGGCCAGAAAAAGCAGCCTCTGTCTTATTCGCTTGAGCTTCCCTCC
CTCTCTGTAGCAGGAGGCATCGCAGCAGGTGAAGTGGGCTTCAGTCTCCA
AAATGCAACAGTGCGTGTGAGTGATAGGAGCCAGCTGCTGATAGCATATC
TGATAAGCACAGCAGCAGCAGCGCTGTTCACAACACTGCAGCCGAGGTTC
ATTCCTCGTGGCGGTGCGATTGTTGATAGCCATGAAACCATGTATCCCGC
TTGGTCAGTGCAGCAGAAAGATTATGCCACGGTTCGGGGAGCCGGTGGGG
AAGTACCCACCGAGCAGGGTAACCAAGGCTTGGGAATGATGCTTCTCGCA
TTGCAGAAAAGCGCGTGGGACTGTCTCGACTTCCCAGGGTCTCCGAAGCG
GGAGTTCAGGCTCGTCAGGGCTAAAGCGGATGCAAAAGCGAAGGAAGGCC
CAATTGAGCACAGTCCCATGTTCGGTGGTATTGTGGATGAGATCGGCAGT
CCTGAGGGGACTGATGGCTGTGGTGGTTGTGGAGGTTgcgggggttgtgg
aggctgcggtggttgtgggggatgcggcggcggcgtcggtgcagctgggg
gctgtggcggcggcggcggcggctgcggcTGTTGATCTTCGACCGATCAT
CGGTGGTGAGCCAACTCAGTCAATAACAAACACAGAATCAGCTACACGGT
GGACCGGTCCGCCGTACAGACGACGCAGTGCGATGGGGATGGTGGCAGTG
TGAGCCTGACCAGTTTGAGCGTCAATTTGGAAGGGCAGTTTGTGTGGCTG
AGGTATGTGATTGCGCGTGCTGGAGAGAAGCTTATTGAGACCGGATACGG
GCAGGTGGTGAACCTTGCGGAGCTGGAGTTGTCGGACGTGCTGAAGCCTG
CCCAATTCCTAGTCTCACAGTTGGCGTAG

Coding sequence (CDS) from alignment at NODE_1148_length_13309_cov_4.323498:7769..10297+

>Gcaud2020.t1 ID=Gcaud2020.t1|Name=Gcaud2020.t1|organism=Gracilaria caudata M_176_S67 male|type=CDS|length=2268bp|location=Sequence derived from alignment at NODE_1148_length_13309_cov_4.323498:7769..10297+ (Gracilaria caudata M_176_S67 male)
ATGAACCTCCTGGCTTTTAACCTGGATGGACGCAAATCGAAGGCAAAGCG
AGGTCTGGCGTCGTCTGTGAAGGCGTTAGTGTCTGTTCTCAATGAAGTCG
ATGAGTACCCGGCTTTGTATTTCAACCACCTCGTGTTGCGGGAGGCCATC
CGCCGCTATGAACATTTATGGATTCCCCTTCTTGCCAATGCCAAGCCCAA
GCAACGCCTGCGTCTCGTTCCGCCGCTTGACGTTGAATGGGTTTGGTTCT
GCCACATGCTCTGTCCGGCCAAATACGCCACTGACTGTGCCGCCATGTGG
CAGGCCGTGCGCCCTAACGAGCCTGATTTCATTTTAGACCATGTGCTTCT
GGGGCCGAAGAAGAGACTGGCCGGAAAATTGGAGGCTGAACAATTGTGGC
GACAGATGTTTCCACACGAGCCTTTCGACAACACGGATGCAATCAAGGAC
GCACCCATGCAAGTGCCCATGAAGCGTGGCTCCGCCTCAGAAGCGTCCCG
CATCTCGTACAATATCGTGGCCGCAGCGGAAAGGCAGATGGTGTTCCAGT
ATCAGGTCGGCATCATGCCGCAATATGAAGAGAACAGCTTTCTGCGGTCC
GCTGTCCGCCGATACAAAAAGTTTCTCAAACTGCAACAGATGCAACCGGA
TAAGATTTGGGTTCCAACATATGATATCGACGTGTGCTGGCACGCTCATA
TGATGCACCCACGTTGGTATCATAGCAACACCGTGAAATTGAAGGGATCA
GTACTGAAACATGATGACACGCTCAACGATAGAGCAACGGGATCCGAGCT
CAACCAGAGGTGGAATGCGACCGCGAGTACATGGAAACAGCTTTTCTCTT
CTCCAGTGCACTTTGCAGGTGGAATGTGGCGGGGAAACGTTGAAACCTCG
GAAAGAGTACAACGACCGGCTCTCGTTGAGAGCATTGTTGCTCTGCAGGC
GGATTGTGCTTCACCAGCAAATGCCGAGTACAAAGTGTGGGATGCTGACA
TGAGCGCATTGGATCTAAGCGCCGGCTTGGACTGGGTTCACATGACAGAG
TGGCGTAGGAAGAATGAGCCTGCGGATCGTAGCAGCCCATGCAGTACCCA
AAACAAAGTGGAACGCTTCACCTGCCTCGGACAAGAAACAGGAGATGCAG
GTATTCTCTTGAGTCGAACGATACATTTTCTGATGTACAAGAATGACGGC
TCCAATGCTGAACCAACCCGTTTTGCCTCTGTAGTGGAGGTGTTCGGAGC
GAAGCCATCTTCAGAGAAACCAGGATACCCGCTCGCAACAGCGCACCATG
TGCTTCCCAACAGTATATGCGGTTGGGAATCGTCCCAAGTCCCGGCTCGC
AGATGTGCTGCATCCGCCACAGAAACTGTTTTTGTTTTGAGGGTTGGCGG
AGAGGACTTTGGCCTCATTGCAGGAGAATGGTATGGTTTTGAACCTCCTC
GGAAAGGGGTTCCTGGAATTCCAGGTAGCAGGGGTGGAAGAGAATGGGGG
GTGCCTGGTATTCCAGGAACGAGGGGAGATCCGGGGCATCTGAATCTGCG
CTTATGGTTACTTGGTGCGAAAACTCGAAGGAGATGGATCAACTTGAGGC
CCGTAGGCCAGAAAAAGCAGCCTCTGTCTTATTCGCTTGAGCTTCCCTCC
CTCTCTGTAGCAGGAGGCATCGCAGCAGGTGAAGTGGGCTTCAGTCTCCA
AAATGCAACAGTGCGTGTGAGTGATAGGAGCCAGCTGCTGATAGCATATC
TGATAAGCACAGCAGCAGCAGCGCTGTTCACAACACTGCAGCCGAGGTTC
ATTCCTCGTGGCGGTGCGATTGTTGATAGCCATGAAACCATGTATCCCGC
TTGGTCAGTGCAGCAGAAAGATTATGCCACGGTTCGGGGAGCCGGTGGGG
AAGTACCCACCGAGCAGGGTAACCAAGGCTTGGGAATGATGCTTCTCGCA
TTGCAGAAAAGCGCGTGGGACTGTCTCGACTTCCCAGGGTCTCCGAAGCG
GGAGTTCAGGCTCGTCAGGGCTAAAGCGGATGCAAAAGCGAAGGAAGGCC
CAATTGAGCACAGTCCCATGTTCGGTGGTATTGTGGATGAGATCGGCAGT
CCTGAGGGGACTGATGGCTGTGGGCAGTTTGTGTGGCTGAGGTATGTGAT
TGCGCGTGCTGGAGAGAAGCTTATTGAGACCGGATACGGGCAGGTGGTGA
ACCTTGCGGAGCTGGAGTTGTCGGACGTGCTGAAGCCTGCCCAATTCCTA
GTCTCACAGTTGGCGTAG