mRNA_F-serratus_M_contig1276.2041.1 (mRNA) Fucus serratus male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_F-serratus_M_contig1276.2041.1
Unique NamemRNA_F-serratus_M_contig1276.2041.1
TypemRNA
OrganismFucus serratus male (Fucus serratus male (Toothed wrack or serrated wrack))
Homology
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BLAST of mRNA_F-serratus_M_contig1276.2041.1 vs. uniprot
Match: A0A6H5JMU8_9PHAE (Protein kinase domain-containing protein n=2 Tax=Ectocarpus TaxID=2879 RepID=A0A6H5JMU8_9PHAE)

HSP 1 Score: 168 bits (425), Expect = 6.440e-48
Identity = 76/105 (72.38%), Postives = 88/105 (83.81%), Query Frame = 1 
Query:    1 MSGVAGSKRFMAPEVCNNLQYNEKADVYSFGIVMWLICTLQKPFSGMSVADHYREVVVGGLRPHLDSRWPTVLNNLLNSCWHPDPDRRPSIVEAREILGE-FVAG 312
            MSG+AGSKRFM+PEVC  L YNEK DVYSF IV+W ICTL+KPF+GMSVADHYREV+ GGLRP LD RWP  L +LL +CWH DPDRRPSI EAR++L + F+AG
Sbjct:  234 MSGMAGSKRFMSPEVCKGLPYNEKVDVYSFAIVLWEICTLRKPFAGMSVADHYREVITGGLRPPLDPRWPGALQHLLQACWHEDPDRRPSISEARDVLQQIFLAG 338
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BLAST of mRNA_F-serratus_M_contig1276.2041.1 vs. uniprot
Match: A0A6H5L2N4_9PHAE (Protein kinase domain-containing protein n=2 Tax=Ectocarpus TaxID=2879 RepID=A0A6H5L2N4_9PHAE)

HSP 1 Score: 151 bits (381), Expect = 9.480e-42
Identity = 67/103 (65.05%), Postives = 81/103 (78.64%), Query Frame = 1 
Query:    1 MSGVAGSKRFMAPEVCNNLQYNEKADVYSFGIVMWLICTLQKPFSGMSVADHYREVVVGGLRPHLDSRWPTVLNNLLNSCWHPDPDRRPSIVEAREILGEFVA 309
            MSG+AGSKRFMAPEVC  L YNEK DVYSF +V+W IC L+KPF+GMS ADHY +VV GGLRP LD+RWP  L +LL +CWH  PD RP++V+AR+ L +  A
Sbjct:  193 MSGMAGSKRFMAPEVCTGLSYNEKVDVYSFALVLWQICALRKPFAGMSEADHYLQVVQGGLRPPLDARWPPQLVHLLQACWHVHPDARPTMVQARDTLRQIFA 295
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BLAST of mRNA_F-serratus_M_contig1276.2041.1 vs. uniprot
Match: A0A6H5K4Q0_9PHAE (Protein kinase domain-containing protein n=2 Tax=Ectocarpus TaxID=2879 RepID=A0A6H5K4Q0_9PHAE)

HSP 1 Score: 129 bits (323), Expect = 2.540e-32
Identity = 57/109 (52.29%), Postives = 74/109 (67.89%), Query Frame = 1 
Query:    1 MSGVAGSKRFMAPEVCNNLQYNEKADVYSFGIVMWLICTLQKPFSGMSVADHYREVVVGGLRPHLDSRWPTVLNNLLNSCWHPDPDRRPSIVEAREILGEFVAGMSGCA 327
            M+G  GS+RFMAPEV + + YNEKAD+YS+GIV+W +C LQKPF+GMS  +H R+V   G+RP LD RWP  L +LL SCWH + + RP   +A E L   +  M   A
Sbjct:  263 MTGQTGSQRFMAPEVFDGMPYNEKADIYSYGIVLWELCALQKPFAGMSSHEHARKVFRHGVRPPLDKRWPDELKSLLRSCWHAEAEERPDAAQAVETLARIIEKMDATA 371
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BLAST of mRNA_F-serratus_M_contig1276.2041.1 vs. uniprot
Match: A0A835ZE86_9STRA (Kinase-like domain-containing protein n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835ZE86_9STRA)

HSP 1 Score: 112 bits (280), Expect = 1.680e-25
Identity = 48/89 (53.93%), Postives = 63/89 (70.79%), Query Frame = 1 
Query:    1 MSGVAGSKRFMAPEVCNNLQYNEKADVYSFGIVMWLICTLQKPFSGMSVADHYREVVVGGLRPHLDSRWPTVLNNLLNSCWHPDPDRRP 267
            M+G  GS R+MAPEV  +L YNEK DVY+FG+++W +   ++ F GMSV D Y+ VV GG+RP LDS WP  L  L++ CWH D D+RP
Sbjct:  326 MTGETGSMRYMAPEVVESLPYNEKVDVYAFGLLLWEMLQYRRVFEGMSVGDFYQRVVNGGIRPPLDSSWPPALRKLIDLCWHADVDKRP 414
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BLAST of mRNA_F-serratus_M_contig1276.2041.1 vs. uniprot
Match: B8CCK4_THAPS (Protein kinase domain-containing protein (Fragment) n=1 Tax=Thalassiosira pseudonana TaxID=35128 RepID=B8CCK4_THAPS)

HSP 1 Score: 103 bits (256), Expect = 2.100e-25
Identity = 47/98 (47.96%), Postives = 65/98 (66.33%), Query Frame = 1 
Query:    1 MSGVAGSKRFMAPEVCNNLQYNEKADVYSFGIVMWLICTLQKPFSGMSVADHYREVVVGGLRPHLDSRWPTVLNNLLNSCWHPDPDRRPSIVEAREIL 294
            MSG  GS R+MAPEV +   YN+KADVYSFGI++W +   +KP+ GM+  + Y  VV GG RP L+ +WP  L+ L+ SCW  +  +RP+  +  EIL
Sbjct:    1 MSGETGSLRYMAPEVADCQPYNQKADVYSFGIILWELVAFKKPYDGMNRDEFYSRVVRGGERPLLNKKWPEDLSELMKSCWDAELVKRPNFSDIVEIL 98
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BLAST of mRNA_F-serratus_M_contig1276.2041.1 vs. uniprot
Match: A0A7S1ZHS7_TRICV (Hypothetical protein n=1 Tax=Trieres chinensis TaxID=1514140 RepID=A0A7S1ZHS7_TRICV)

HSP 1 Score: 104 bits (260), Expect = 5.200e-25
Identity = 50/107 (46.73%), Postives = 63/107 (58.88%), Query Frame = 1 
Query:    1 MSGVAGSKRFMAPEVCNNLQYNEKADVYSFGIVMWLICTLQKPFSGMSVADHYREVVVGGLRPHLDSRWPTVLNNLLNSCWHPDPDRRPSIVEAREILGEFVAGMSG 321
            MSG  GS R+MAPEV +   YN KADVYSFGI++W I + QKPF GM   + Y  VV GG RP +  +WP  L  ++  CW  DP  RP+  E R  L   +   +G
Sbjct:   43 MSGETGSLRYMAPEVADCRPYNHKADVYSFGIILWEIVSFQKPFDGMGRDEFYARVVHGGERPAIGKKWPKELVEIMTRCWDYDPKFRPTFHEVRTKLDALLEKETG 149
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BLAST of mRNA_F-serratus_M_contig1276.2041.1 vs. uniprot
Match: D7FS28_ECTSI (Protein kinase domain-containing protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FS28_ECTSI)

HSP 1 Score: 108 bits (271), Expect = 9.390e-25
Identity = 49/98 (50.00%), Postives = 63/98 (64.29%), Query Frame = 1 
Query:    1 MSGVAGSKRFMAPEVCNNLQYNEKADVYSFGIVMWLICTLQKPFSGMSVADHYREVVVGGLRPHLDSRWPTVLNNLLNSCWHPDPDRRPSIVEAREIL 294
            M+G  GS R+MAPEV  +L YNEK DVY+FG+++W +   ++ F GM V D Y  VV  G RP+LDS WP  L  L++ CWH D DRRP+     E L
Sbjct:  179 MTGETGSMRYMAPEVVESLPYNEKVDVYAFGLLLWEMLEDRRVFDGMGVTDFYERVVNAGARPNLDSAWPQDLRKLISQCWHADVDRRPNFRTIAERL 276
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BLAST of mRNA_F-serratus_M_contig1276.2041.1 vs. uniprot
Match: A0A6H5L2R6_9PHAE (Protein kinase domain-containing protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5L2R6_9PHAE)

HSP 1 Score: 108 bits (271), Expect = 2.840e-24
Identity = 49/98 (50.00%), Postives = 63/98 (64.29%), Query Frame = 1 
Query:    1 MSGVAGSKRFMAPEVCNNLQYNEKADVYSFGIVMWLICTLQKPFSGMSVADHYREVVVGGLRPHLDSRWPTVLNNLLNSCWHPDPDRRPSIVEAREIL 294
            M+G  GS R+MAPEV  +L YNEK DVY+FG+++W +   ++ F GM V D Y  VV  G RP+LDS WP  L  L++ CWH D DRRP+     E L
Sbjct:  392 MTGETGSMRYMAPEVVESLPYNEKVDVYAFGLLLWEMLEDRRVFDGMGVTDFYERVVNAGARPNLDSAWPQDLRKLISQCWHADVDRRPNFRTIAERL 489
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BLAST of mRNA_F-serratus_M_contig1276.2041.1 vs. uniprot
Match: A0A7S1ZVA0_9STRA (Hypothetical protein n=1 Tax=Ditylum brightwellii TaxID=49249 RepID=A0A7S1ZVA0_9STRA)

HSP 1 Score: 102 bits (255), Expect = 9.540e-24
Identity = 49/112 (43.75%), Postives = 68/112 (60.71%), Query Frame = 1 
Query:    1 MSGVAGSKRFMAPEVCNNLQYNEKADVYSFGIVMWLICTLQKPFSGMSVADHYREVVVGGLRPHLDSRWPTVLNNLLNSCWHPDPDRRPSIVEAREILGEFVAGMSGCAEQR 336
            MSG  GS R+MAPEV  +L YN+KADVYSFGI++W +   +KP+ GM+  + Y  VV G  RP ++ +WP  L  L+  CW  D   RPS  E  E L   ++   G +E++
Sbjct:  104 MSGQTGSLRYMAPEVAKSLPYNQKADVYSFGIILWELNAYKKPYEGMNKDEFYMRVVEGKERPPINKKWPEELVQLMQECWSFDARLRPSFHEIVERLDAMLSKEKGGSEKK 215
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BLAST of mRNA_F-serratus_M_contig1276.2041.1 vs. uniprot
Match: B5YNN3_THAPS (Predicted protein (Fragment) n=1 Tax=Thalassiosira pseudonana TaxID=35128 RepID=B5YNN3_THAPS)

HSP 1 Score: 99.8 bits (247), Expect = 1.420e-23
Identity = 45/87 (51.72%), Postives = 57/87 (65.52%), Query Frame = 1 
Query:    4 SGVAGSKRFMAPEVCNNLQYNEKADVYSFGIVMWLICTLQKPFSGMSVADHYREVVVGGLRPHLDSRWPTVLNNLLNSCWHPDPDRR 264
            SG+AG++R+MAPEV   + Y   ADVYSFGI++W + +L+  F G S   HY+EVVV G RP +   WP V  NLL  CW PDP  R
Sbjct:   52 SGMAGTRRYMAPEVAQVIHYGLPADVYSFGILVWEMLSLKPAFFGYSREKHYKEVVVEGKRPKIPKSWPFVTRNLLERCWAPDPSER 138
The following BLAST results are available for this feature:
BLAST of mRNA_F-serratus_M_contig1276.2041.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Fucus serratus MALE vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A6H5JMU8_9PHAE6.440e-4872.38Protein kinase domain-containing protein n=2 Tax=E... [more]
A0A6H5L2N4_9PHAE9.480e-4265.05Protein kinase domain-containing protein n=2 Tax=E... [more]
A0A6H5K4Q0_9PHAE2.540e-3252.29Protein kinase domain-containing protein n=2 Tax=E... [more]
A0A835ZE86_9STRA1.680e-2553.93Kinase-like domain-containing protein n=1 Tax=Trib... [more]
B8CCK4_THAPS2.100e-2547.96Protein kinase domain-containing protein (Fragment... [more]
A0A7S1ZHS7_TRICV5.200e-2546.73Hypothetical protein n=1 Tax=Trieres chinensis Tax... [more]
D7FS28_ECTSI9.390e-2550.00Protein kinase domain-containing protein n=1 Tax=E... [more]
A0A6H5L2R6_9PHAE2.840e-2450.00Protein kinase domain-containing protein n=1 Tax=E... [more]
A0A7S1ZVA0_9STRA9.540e-2443.75Hypothetical protein n=1 Tax=Ditylum brightwellii ... [more]
B5YNN3_THAPS1.420e-2351.72Predicted protein (Fragment) n=1 Tax=Thalassiosira... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
F-serratus_M_contig1276contigF-serratus_M_contig1276:254064..254614 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Fucus serratus MALE vs UniRef902022-09-19
OGS1.0 of Fucus serratus male2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score169.5
Seed ortholog evalue9.1e-40
Seed eggNOG ortholog2880.D7FPX1
KEGG koko:K14304
KEGG TC1.I.1
KEGG Pathwayko03013,map03013
KEGG ModuleM00427
Hectar predicted targeting categoryother localisation
GOsGO:0000323,GO:0000902,GO:0000904,GO:0003674,GO:0003824,GO:0004672,GO:0004674,GO:0005488,GO:0005515,GO:0005575,GO:0005622,GO:0005623,GO:0005737,GO:0005764,GO:0005765,GO:0005768,GO:0005770,GO:0005773,GO:0005774,GO:0005794,GO:0005795,GO:0006464,GO:0006468,GO:0006793,GO:0006796,GO:0006807,GO:0006810,GO:0006897,GO:0006914,GO:0006928,GO:0006935,GO:0006950,GO:0006979,GO:0006996,GO:0007005,GO:0007017,GO:0007018,GO:0007154,GO:0007165,GO:0007275,GO:0007399,GO:0007409,GO:0007411,GO:0007528,GO:0008088,GO:0008104,GO:0008150,GO:0008152,GO:0009056,GO:0009267,GO:0009605,GO:0009653,GO:0009894,GO:0009987,GO:0009991,GO:0010008,GO:0010506,GO:0010721,GO:0010769,GO:0010771,GO:0010941,GO:0010970,GO:0010975,GO:0010977,GO:0012505,GO:0016020,GO:0016043,GO:0016049,GO:0016192,GO:0016197,GO:0016241,GO:0016301,GO:0016310,GO:0016482,GO:0016740,GO:0016772,GO:0016773,GO:0017016,GO:0017137,GO:0018105,GO:0018193,GO:0018209,GO:0019222,GO:0019538,GO:0019899,GO:0022008,GO:0022603,GO:0022604,GO:0023052,GO:0030030,GO:0030100,GO:0030154,GO:0030182,GO:0030424,GO:0030425,GO:0030705,GO:0031090,GO:0031175,GO:0031267,GO:0031323,GO:0031329,GO:0031344,GO:0031345,GO:0031410,GO:0031594,GO:0031667,GO:0031668,GO:0031669,GO:0031902,GO:0031982,GO:0031984,GO:0032418,GO:0032501,GO:0032502,GO:0032879,GO:0032886,GO:0032989,GO:0032990,GO:0033036,GO:0033043,GO:0033267,GO:0033554,GO:0034613,GO:0034976,GO:0035556,GO:0036211,GO:0036465,GO:0036477,GO:0040007,GO:0040011,GO:0042147,GO:0042221,GO:0042330,GO:0042594,GO:0042995,GO:0043005,GO:0043025,GO:0043170,GO:0043195,GO:0043198,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0043412,GO:0043679,GO:0044087,GO:0044088,GO:0044237,GO:0044238,GO:0044248,GO:0044260,GO:0044267,GO:0044297,GO:0044306,GO:0044422,GO:0044424,GO:0044431,GO:0044433,GO:0044437,GO:0044440,GO:0044444,GO:0044446,GO:0044456,GO:0044463,GO:0044464,GO:0045202,GO:0045595,GO:0045596,GO:0045664,GO:0045665,GO:0046907,GO:0048311,GO:0048312,GO:0048468,GO:0048488,GO:0048489,GO:0048519,GO:0048523,GO:0048588,GO:0048589,GO:0048666,GO:0048667,GO:0048675,GO:0048699,GO:0048731,GO:0048812,GO:0048814,GO:0048846,GO:0048856,GO:0048858,GO:0048869,GO:0050767,GO:0050768,GO:0050773,GO:0050774,GO:0050789,GO:0050793,GO:0050794,GO:0050803,GO:0050807,GO:0050808,GO:0050896,GO:0051020,GO:0051049,GO:0051093,GO:0051128,GO:0051129,GO:0051179,GO:0051234,GO:0051239,GO:0051241,GO:0051493,GO:0051640,GO:0051641,GO:0051645,GO:0051646,GO:0051648,GO:0051649,GO:0051650,GO:0051656,GO:0051683,GO:0051716,GO:0051960,GO:0051961,GO:0060284,GO:0060341,GO:0060548,GO:0060560,GO:0060627,GO:0061174,GO:0061564,GO:0061845,GO:0061919,GO:0065007,GO:0065008,GO:0070507,GO:0070727,GO:0071212,GO:0071496,GO:0071704,GO:0071840,GO:0090128,GO:0097447,GO:0097458,GO:0097479,GO:0097480,GO:0097485,GO:0097708,GO:0098588,GO:0098657,GO:0098693,GO:0098791,GO:0098793,GO:0098805,GO:0098852,GO:0099003,GO:0099111,GO:0099174,GO:0099504,GO:0120025,GO:0120035,GO:0120036,GO:0120038,GO:0120039,GO:0140096,GO:0150034,GO:1900242,GO:1901214,GO:1901215,GO:1901564,GO:1902115,GO:1902284,GO:1903421,GO:1990033,GO:1990138,GO:2000026,GO:2000171,GO:2000331,GO:2000785
EggNOG free text desc.protein kinase activity
EggNOG OGsCOG0515@1,KOG0192@2759
COG Functional cat.KLT
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko00002,ko03019,ko03036
Exons1
Model size551
Cds size339
Stop1
Start1
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622929209.7346182-CDS-F-serratus_M_contig1276:254063..2544021622929209.7346182-CDS-F-serratus_M_contig1276:254063..254402Fucus serratus maleCDSF-serratus_M_contig1276 254064..254402 +
1690962751.4550128-CDS-F-serratus_M_contig1276:254063..2544021690962751.4550128-CDS-F-serratus_M_contig1276:254063..254402Fucus serratus maleCDSF-serratus_M_contig1276 254064..254402 +


The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622929209.751322-UTR-F-serratus_M_contig1276:254402..2546141622929209.751322-UTR-F-serratus_M_contig1276:254402..254614Fucus serratus maleUTRF-serratus_M_contig1276 254403..254614 +
1690962751.4675126-UTR-F-serratus_M_contig1276:254402..2546141690962751.4675126-UTR-F-serratus_M_contig1276:254402..254614Fucus serratus maleUTRF-serratus_M_contig1276 254403..254614 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_F-serratus_M_contig1276.2041.1prot_F-serratus_M_contig1276.2041.1Fucus serratus malepolypeptideF-serratus_M_contig1276 254064..254402 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_F-serratus_M_contig1276.2041.1

>prot_F-serratus_M_contig1276.2041.1 ID=prot_F-serratus_M_contig1276.2041.1|Name=mRNA_F-serratus_M_contig1276.2041.1|organism=Fucus serratus male|type=polypeptide|length=113bp
MSGVAGSKRFMAPEVCNNLQYNEKADVYSFGIVMWLICTLQKPFSGMSVA
DHYREVVVGGLRPHLDSRWPTVLNNLLNSCWHPDPDRRPSIVEAREILGE
FVAGMSGCAEQR*
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mRNA from alignment at F-serratus_M_contig1276:254064..254614+

Legend: CDSpolypeptideUTR
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_F-serratus_M_contig1276.2041.1 ID=mRNA_F-serratus_M_contig1276.2041.1|Name=mRNA_F-serratus_M_contig1276.2041.1|organism=Fucus serratus male|type=mRNA|length=551bp|location=Sequence derived from alignment at F-serratus_M_contig1276:254064..254614+ (Fucus serratus male)
ATGTCTGGAGTGGCTGGCAGCAAGCGGTTCATGGCGCCGGAGGTGTGCAA
CAACTTGCAGTACAACGAAAAAGCGGACGTGTACTCCTTCGGCATCGTGA
TGTGGCTGATCTGTACGCTCCAAAAGCCGTTCTCCGGCATGAGCGTAGCG
GATCACTACCGAGAGGTCGTCGTGGGCGGATTGCGGCCTCACTTGGACTC
TAGGTGGCCGACGGTCCTCAACAACCTCTTGAACTCTTGTTGGCACCCGG
ATCCGGACCGGCGACCCTCCATCGTTGAAGCGCGAGAAATACTAGGGGAA
TTTGTGGCAGGAATGAGCGGTTGCGCTGAGCAGAGATGATTACCAGAAGC
TGCATCCACAGCAAAAAGCGAAGTCGCCGCCTCGAGCCCGAGGGTGCTTT
GTGTCCCGCCCGTTTCCCCAAACGGTAGAACTCAGCGCATAAACTTTTTC
CCATCCGAGAGGAAAGGACTGCGCGGGCATGGTACAATGTAGAGGCACAT
ATATTGGGGTGATTTCCAAAAATAACTCAAGCGAAGGGGGGCATCGAGGG
C
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Coding sequence (CDS) from alignment at F-serratus_M_contig1276:254064..254614+

>mRNA_F-serratus_M_contig1276.2041.1 ID=mRNA_F-serratus_M_contig1276.2041.1|Name=mRNA_F-serratus_M_contig1276.2041.1|organism=Fucus serratus male|type=CDS|length=678bp|location=Sequence derived from alignment at F-serratus_M_contig1276:254064..254614+ (Fucus serratus male)
ATGTCTGGAGTGGCTGGCAGCAAGCGGTTCATGGCGCCGGAGGTGTGCAA
CAACTTGCAGTACAACGAAAAAGCGGACGTGTACTCCTTCGGCATCGTGA
TGTGGCTGATCTGTACGCTCCAAAAGCCGTTCTCCGGCATGAGCGTAGCG
GATCACTACCGAGAGGTCGTCGTGGGCGGATTGCGGCCTCACTTGGACTC
TAGGTGGCCGACGGTCCTCAACAACCTCTTGAACTCTTGTTGGCACCCGG
ATCCGGACCGGCGACCCTCCATCGTTGAAGCGCGAGAAATACTAGGGGAA
TTTGTGGCAGGAATGAGCGGTTGCGCTGAGCAGAGATGAATGTCTGGAGT
GGCTGGCAGCAAGCGGTTCATGGCGCCGGAGGTGTGCAACAACTTGCAGT
ACAACGAAAAAGCGGACGTGTACTCCTTCGGCATCGTGATGTGGCTGATC
TGTACGCTCCAAAAGCCGTTCTCCGGCATGAGCGTAGCGGATCACTACCG
AGAGGTCGTCGTGGGCGGATTGCGGCCTCACTTGGACTCTAGGTGGCCGA
CGGTCCTCAACAACCTCTTGAACTCTTGTTGGCACCCGGATCCGGACCGG
CGACCCTCCATCGTTGAAGCGCGAGAAATACTAGGGGAATTTGTGGCAGG
AATGAGCGGTTGCGCTGAGCAGAGATGA
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