prot_F-serratus_M_contig864.20012.1 (polypeptide) Fucus serratus male

BLAST of mRNA_F-serratus_M_contig864.20012.1 vs. uniprot
Match: A0A6H5K229_9PHAE (Subtilisin n=2 Tax=Ectocarpus TaxID=2879 RepID=A0A6H5K229_9PHAE)

HSP 1 Score: 744 bits (1921), Expect = 4.580e-251
Identity = 454/965 (47.05%), Postives = 605/965 (62.69%), Query Frame = 0

Query:   79 LALVEQLLSTTAQEDFGGRENVYEAAIEWSRNRFFHKNINENHPATAGEDYGTTYIVCSNSPRETLVRLESSLSPASVRKLAVGAEFGACFLATAASPDVAKAVYEELCGRGLATYVMIYPSALKLAPGLLDHSLAHTTSSYDSGAQ--LATMHGTKMRIDNVGGLTVELSPGLLSARDTRYEAYMSDLWEDILSTSINIWATNFWSDPAMSGGKHLNTVEGSLMRQEWTRAADLVHESSKARRRSPGSACGWDSIKMHQAGDDTIIFTGLDHLLASRKVPV---ESEGAEFQMACFMSLVSLLTSRAEVLRVAPYHHKTVLNAVAKAISHSATTDTSETTLTAAGLDGTGEVIQVIDSGLDQESCFFRDSDGETVELGYYFDEVAVISSTYA----LPLFESGGSFPVDLDRRKIVQYISLVRSDVDGENQPYGDYTSTILGQTFPWYSAEGFATETSGGHGSHTAGTAAGSAIGS---TDSCSGDDELGCLGGCLNSSYV-DQLMSNGFF----NVEVFCPKFDCNNVYADSEIYETCLSDDVEETVSANGGVAPGAKLAIFDTSVDGTTVWAELAGNGLWEASNETGSVVHTNSWGGNSDCTVDATAIAYDTFMYQNPEHLLVFSAGNDGNLSDSSCSVGSPAVAKNVLTVGSSSSGQTRWTFTGEDGEPLESIYDTMSDIDTVSYFSSQGPTEDGRIKPEIVAPGDNVLSASSAGEDGETCELELRSGTSMAAPVVAAAAAMIRQYFKDDSFYVSDLKARELCDATTTTISSTNSSTSTSFMCSTFNASAATMKAMLINSANLMGGSSEPDGSRGFGRVHLDAGLPLGGIGDTGLFVADSSDTGVSQGTEDTYTFETVDSDTG-EFRATLAWIDPPATTSSSSQLMHDLDLLVTSPSGQTYTMWSSGETDTSNVIERVVVSADDFDDEESGTWTVSVSANDIVTESQPYSLVVTGPFGNGT 1025
            L LVE  L+TTA++DF G    +EAA  W+  R          P     +YG          RET  RL+  LSP +VR ++   + GACFLATA+    A A+ E+    GL ++   +PSALKLAPGL+DHS +       S  Q  L   HG  MR+ NV GL V+L+P  L AR    E+++ DL  D++S S+++ +TNFWSDP M+GG+HL++  G++  ++W++AA +VHE S+A   SPG  C WD + + QAGDD+++ TGLDHLL S +      E E  E  +ACFM LVS    R EVLRV+P H K VLN  A+ I  SA+   ++T LT AGLDGTGEVIQV+D+GLD+ SCFF D  GE V+ GY+F+E+ V S+  +     P+FE+G  F    +RRK++QYI LV  D D     Y  + ST  G  +P+   + F  + S GHG+HTAG+AAG+ +     T +CSGD+ LGC+GGC++ S   D L+S        +++  CP + C+         + CLSD+V+ET++ NGG+A GAKL+IFD   +   +  E+ GNG+WE   E G  VH+ S GG++ CT   + I YD FMY+NPE+LL+F+AGNDG    S C++ SPA+ KNVL VG+S+SG TR   T E G          + ID V+ FSS G T DGRIKPE+VAPGD V SA+S G D  +C L    GTSM+ P+VA AAAM+RQYF D++FY +D+ AR  C              S  F+C  F+ SAAT+KA+LINSANLMG SS+PD  RGFGR+HL+ G+PL G GD  LFVAD+ DT +++ T   Y F+ VD+D G E RATL+WID PAT++S+ QL++DLDL+V SPSG  Y MW S  TD  NV ERV+V ADD    E+GTWTV V +N +VT+SQ YSLVV G    GT
Sbjct:   34 LELVENGLATTAKDDFNGHARAFEAAATWNGERLRRSQDGTRAPHLCCAEYG--------HGRETFSRLKQFLSPDAVRVVSHSEDHGACFLATASHAQ-ASAIMEDQEQFGLESFSP-FPSALKLAPGLVDHSESREVEGEPSSGQDRLGARHGGTMRMSNVEGLNVKLTPDTLPARSAGAESFIIDLLGDLMSASVDLHSTNFWSDPVMAGGEHLSSHGGAVRARDWSKAASVVHELSEAAGTSPGDICSWDEVALIQAGDDSLLVTGLDHLLFSGRGAGGSHEEESTELHVACFMGLVSFFAGRPEVLRVSPRHTKRVLNEAARGIIQSASA--TDTPLTDAGLDGTGEVIQVVDTGLDETSCFFIDDGGEEVDHGYFFEELFVGSTGSSQLIYYPVFEAG-DFTYTEERRKVIQYIELVNPDSD-----YVAF-STTQGGVYPFLPPDEFGQDDSAGHGTHTAGSAAGATLNDPAETLTCSGDETLGCVGGCIDESETTDDLVSYAIQIALPDLDRLCPMYGCDGST------DPCLSDEVDETLTKNGGMAQGAKLSIFDVFFEVFGL-IEVLGNGVWEPCLEAGCKVHSLSLGGDTLCTPGESEILYDDFMYENPENLLIFAAGNDGETERSVCTMASPAIGKNVLAVGASTSGDTRVIST-EGG----------AGIDMVADFSSYGFTTDGRIKPEVVAPGDMVYSAASDGTDSHSCRLWAYQGTSMSCPIVAGAAAMVRQYFVDETFYTADVTARGFC--------------SDGFICEGFSPSAATVKALLINSANLMGESSQPDAYRGFGRIHLEQGMPLEGEGDLALFVADALDTSIAEDTVQEYLFD-VDADAGLELRATLSWIDYPATSTSAMQLVNDLDLVVVSPSGTVYRMWGSDVTDNRNVNERVIVDADDV---ETGTWTVLVYSNSLVTDSQSYSLVVNGAISAGT 943          

BLAST of mRNA_F-serratus_M_contig864.20012.1 vs. uniprot
Match: A0A6H5JQT4_9PHAE (Subtilisin n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JQT4_9PHAE)

HSP 1 Score: 722 bits (1864), Expect = 1.710e-241
Identity = 445/1017 (43.76%), Postives = 615/1017 (60.47%), Query Frame = 0

Query:   66 ATQSSGRSGSSLP---LALVEQLLSTTAQEDFGGRENVYEAAIEWSRNRFFHKNINENHPATAGEDYGTTYIVCSNSPRETLVRLESSLSPASVRKLAVGAEFGACFLATAASPDVAKAVYEELCGRGLATYVMIYPSALKLAPGLLDHSLAH-----TTSSYDSGAQLATMHGTKMRIDNVGGLTVELSPGLLSARDTRYEAYMSDLWEDILSTSINIWATNFWSDPAMSGGKHLNTVEGSLMRQEWTRAADLVHESSKARRRSPGSACGWDSIKMHQAGDDTIIFTGLDHLLAS-RKVPVESEG---AEFQMACFMSLVSLLTSRAEVLRVAPYHHKTVLNAVAKAISHSATTDTSETTLTAAGLDGTGEVIQVIDSGLDQESCFF-RDSDGETVELGYYFDEVAV----ISSTYALPLFES--------------------------GGSFPVDLDRRKIVQYISLVRSDVDGENQPYGDYTSTILGQTFPWYSAEGFATETSGGHGSHTAGTAAGSAIGSTD---SCSGDDELGCLGGCLNSSYVDQLMSNGFFNVEV-----FCPKF---DCNNVYADSEIYETCLSDDVEETVSANGGVAPGAKLAIFDTSVDGTTVWAELAGNGLWEASNETGSVVHTNSWGGNSDCTVDATAIAYDTFMYQNPEHLLVFSAGNDGNLSDSSCSVGSPAVAKNVLTVGSSSSGQTRWTFTGEDGEPLESIYDTMSDIDTVSYFSSQGPTEDGRIKPEIVAPGDNVLSASSAGE-DGETCELELRSGTSMAAPVVAAAAAMIRQYFKDDSFYVSDLKARELCDATTTTISSTNSSTSTSFMCSTFNASAATMKAMLINSANLMGGSSEPDGSRGFGRVHLDAGLPLGGIGDTGLFVADSSDTGVSQGTEDTYTFETVDSDTGEFRATLAWIDPPATTSSSSQLMHDLDLLVTSPSGQTYTMWSSGETDTSNVIERVVVSADDFDDEESGTWTVSVSANDIVTESQPYSLVVTGPFGNGTRV 1027
            A  S G  G SLP   L LVE  LSTTA++DF G    +EAA  W+  R  H+      P     +YG          RET  RL+  LSP +VR ++   + GACF A+A+    A A+ E+    GL ++   +PSALKLAPGL+DHS  H     TT+  D   +L+  HG +MR+ NV GL++EL+PG L++R    EA++SD   D++  S+++ +TNFWSD  M GG+ L+   G++  ++W++AA +VHE S+A   SPG  C W+ + +H A  D ++ +GLDHLL S R     S+G   AE  +ACFM LVS L S+ EVLRVAP   + +LNA A+A   SAT   ++T LT AGLDGTG+V+QVIDSG+D+ SCFF  D  G+ V  G+YF+E  +     SS+++ P  +                           GG F +  DRRKI+QYI+L++SD D E   +GD  +T LGQ +PW  A+ F  +   GHG+H AG+ AG+ + +     SC   + + C+G C++    + +      NVE+      CP F   DC+ +       E CLSDDV ET++ NGG+A GAKLAIFD   D   + +   GNGLWE   E    +H+NS+GG+ +C++    + YD +M  NPE+LL+F+AGNDG    S+C++ SPA+AKNVL VG+S+SG TR + T   G            IDTV+ FSS GPT D RIKPE++APGD + SA+S G  D  +C L   +GTSM+ P+VA A+AMIRQYF +++F+ +D+ AR  CD                  C  F+ S+AT+KA++INSANLMGGSSEPDGSRGFGR+HL+AG+PL G G   LFV+DS+ T + + T   Y F+   +   + R TL+W+DPPATT S+ QL+HDLDL+V SP+G  YTMW+SG TD++NV ERV+V A D    E+GTW+V + A+++ T+ Q YSLVV G    GT V
Sbjct:    8 AGASVGWPGKSLPYPSLELVENALSTTAKDDFNGHARAFEAAATWNGERLRHRQDETRAPHLCCAEYG--------HGRETFSRLKQFLSPEAVRVVSHSEDLGACFFASASHAQ-ASAIIEDQEQFGLESFSP-FPSALKLAPGLVDHSQRHDKDKETTTGLD---RLSARHGARMRMSNVEGLSIELTPGTLASRSVEAEAFISDTLGDLMLASVDLHSTNFWSDQNMGGGEQLSFPGGAVRARDWSKAASVVHELSEAAGTSPGDICSWNRVSVHHAEGDVLLVSGLDHLLHSGRGAGGRSDGGESAELHIACFMGLVSFLASKPEVLRVAPRQSQALLNASARANIQSATV--TDTPLTDAGLDGTGQVVQVIDSGVDESSCFFAHDESGDHVPHGHYFEEWGINPPLTSSSFSSPTSDDDDSLGWPRATDDHRTGSYYQIAEEFDGGDFTIFPDRRKIIQYINLIKSD-DTEGS-HGDSFTTSLGQQYPWLPADRFEYDYEAGHGTHVAGSVAGATLNTPAELISCKDTETMSCVGACVDDDEANDVDDLATPNVEIVDLDRLCPLFSDYDCDVLET-----EKCLSDDVAETLTNNGGMAQGAKLAIFDAFYDNFGL-SPFVGNGLWEPCAEADCKIHSNSYGGDFECSLGPNDVLYDGWMVSNPENLLIFAAGNDGERRRSTCTMSSPAIAKNVLAVGASTSGDTRLSATPGLG------------IDTVAVFSSYGPTSDSRIKPEVLAPGDAIYSAASDGSMDSHSCRLWAYAGTSMSTPIVAGASAMIRQYFMNETFFATDMTARGFCDEWAG--------------CEAFSPSSATLKALMINSANLMGGSSEPDGSRGFGRIHLEAGMPLRGEGSLALFVSDSNYTSIPERTRQEYNFDVNATARLDLRVTLSWLDPPATTFSAVQLVHDLDLVVISPNGTRYTMWASGVTDSANVNERVIVDAGDV---ETGTWSVWIWADNLTTDVQSYSLVVNGAISPGTGV 972          

BLAST of mRNA_F-serratus_M_contig864.20012.1 vs. uniprot
Match: D8LNG6_ECTSI (Subtilisin n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LNG6_ECTSI)

HSP 1 Score: 694 bits (1791), Expect = 6.290e-232
Identity = 442/1006 (43.94%), Postives = 597/1006 (59.34%), Query Frame = 0

Query:   79 LALVEQLLSTTAQEDFGGRENVYEAAIEWSRNRFFHKNINENHPATAGEDYGTTYIVCSNSPRETLVRLESSLSPASVRKLAVGAEFGACFLATAASPDVAKAVYEELCGRGLATYVMIYPSALKLAPGLLDHSLAHTTSSYDSGA----QLATMHGTKMRIDNVGGLTVELSPGLLSARDTRYEAYMSDLWEDILSTSINIWATNFWSDPAMSG-GKHLNTVEGSLMRQEWTRAADLVHESSKARRRSPGSACGWDSIKMHQAGDDTIIFTGLDHLLASRKVPV---ESEGAEFQMACFMSLVSLLTSRAEVLRVAPYHHKTVLNAVAKAISHSATTDTSETTLTAAGLDGTGEVIQVIDSGLDQESCFFRDSDGETVELGYYFDEV---AVISSTYALPLFESGGSFPVDLDRRKIVQYISLVRSDVDGENQPYGDYTSTIL-GQTFPWYSAEGFATETSGGHGSHTAGTAAGSAIGS---TDSCSGDDELGCLGGCLNS-SYVDQLMS----NGFFNVEVFCPKFDCNNVYADSEIYETCLSDDVEETVSANGGVAPGAKLAIFDTSVDGTTVWAELAGNGLWEASNETGSVVHTNSWGGNSDCTVDATAIAYDTFMYQNPEHLLVFSAGNDGNLSDSSCSVGSPAVAKNVLTVGSSSSGQTRWTFT-GEDGEPLESIYDTMSDIDTVSYFSSQGPTEDGRIKPEIVAPGDNVLSASSAGEDGETCELELRSGTSMAAPVVAAAAAMIRQYFKDDSFYVSDLKARELCDATTTTISSTNSSTSTSFMCSTFNASAATMKAMLINSANLMGGSSEPDGSRGFGRVHLDAGLPLGGIGDTGLFVADSSDTGVSQGTEDTYTFETVDSDTG-EFRATLAWIDPPATTSSSSQLMHDLDLLVTSPSGQTYTMWSSGETDTSNVIERVVVSADDFDDEESGTWTVSVSANDIVTESQPYSLVVTGPFGNGTRVDDSSMAAASMYRSMALAKSVLWVGVGLASALLAAS 1062
            L LVE  L TTA++DF G    +EAA  W+  R  H       P  A   YG  +   S        RL+  LSP +VR ++   + GACF A+A+    A A+ E+    GL  +   +PS LKLAPGL+DH+ +H  +  ++ +    +L+  HG +MR  NV GL+VEL+PG L+AR +  +++++ +  D++S S+++ +TNFWSDP M G G+HL++  G++  ++W +AA +VHE S+A    PG  C W+ I +H AGDD+++ +GLDHLL S +      E E  E  +ACFM LVS L  R EVLRV+P   K VLNA A+ +  SA+   ++T LT AGLDGTGE+IQVIDSGLD+ SC+F D  GE V+ GYYFDE+   AV SS+Y+                                      D T+TI  G  F +Y     A     GHG+HTAG+AAG+ +     T +C+  D L C+G C++     D L+S        +++  CP + C+N         TCLSDDV ET+S NGG+A GAKLAIFD    G    + + GN LWE   E G  +H+NS G + +C++ +  I  D FMY+N E+LL+F+AGN+G +  S+C++ +P + KN L +G+S+SG TR   T G  G            IDTV+ FSS G T D RIKPE+VAPGD+V SA+S G D  +C L    GTSM+ P+VA AAAMIRQYF D++FY +D+ AR  C              S  F+C  F+ SAAT+KA+LINSANLMGGSSEPDG RGFGRVHL+AG+PL G GD  LFVAD+ DT ++ GT + Y F+ VD+D G + RATL+WIDPPAT +S+ QL+H+LDL+V SP G TY MW S  TD  NV ERV+V A D     +GTWTV V AN++VT+SQ YSLVV G    GT    S  ++  +  S   A S    G G+    L A+
Sbjct:   29 LELVENALLTTAKDDFKGHVGAFEAAATWNGERLRHSQEETRAPHLACARYGDGHEASS--------RLKQFLSPEAVRVVSHSEDHGACFFASASHAQAA-AIVEDQEQFGLENFSP-FPSPLKLAPGLVDHTESHDEAEEEARSSGLDRLSARHGARMRKPNVEGLSVELTPGTLAARSSEAKSFINHMLGDLMSASVDLHSTNFWSDPGMDGAGEHLSSPAGAVRARDWRKAASVVHELSEAASTGPGDICSWNRIFVHHAGDDSLLVSGLDHLLFSGRGAGGSHEEESTELHVACFMGLVSFLAGRPEVLRVSPRPTKRVLNAAARGVIQSASA--TDTPLTDAGLDGTGEIIQVIDSGLDETSCYFIDDSGEEVDHGYYFDELGRAAVYSSSYS-------------------------------------SDETATIFNGGDFSYYPERRKA-----GHGTHTAGSAAGATLNDPAETITCNVTDTLSCVGACIDLLDATDDLVSISSQTDGTDLDRLCPMYGCDN-----STDTTCLSDDVPETLSNNGGMAQGAKLAIFDVFYGGYDASSSI-GNNLWEVCTEAGCKLHSNSLGADFECSIASWDILNDQFMYENAENLLIFAAGNEGGIERSTCTINTPGIGKNSLAIGASTSGDTRVISTVGATG------------IDTVAEFSSWGFTTDDRIKPEVVAPGDSVYSAASDGTDTHSCRLWAYEGTSMSCPIVAGAAAMIRQYFVDETFYTADVTARGFC--------------SDGFICEGFSPSAATVKALLINSANLMGGSSEPDGFRGFGRVHLEAGMPLEGEGDLVLFVADALDTSIAGGTLEEYLFD-VDADAGLDLRATLSWIDPPATATSAVQLVHNLDLVVVSPDGTTYRMWGSDTTDNRNVNERVIVDATDV---VTGTWTVWVWANNLVTDSQSYSLVVNGAISPGTGEGASGSSSFEVSSSATSASSEDGTGAGVTGGSLVAA 944          

BLAST of mRNA_F-serratus_M_contig864.20012.1 vs. uniprot
Match: A0A2I6U1S5_SACJA (Subtilisin n=1 Tax=Saccharina japonica TaxID=88149 RepID=A0A2I6U1S5_SACJA)

HSP 1 Score: 658 bits (1698), Expect = 2.060e-218
Identity = 425/994 (42.76%), Postives = 585/994 (58.85%), Query Frame = 0

Query:   71 GRSGSSLP---LALVEQLLSTTAQEDFGGRENVYEAAIEWSRNRFFHKNINENHPATAGEDYGTTYIVCSNSPR--ETLVRLESSLSPASVRKLAVGAEFGACFLATAASPDVAKAVYEELCGRGLATYVMIYPSALKLAPGLLDHSLAHTTSSYDSGAQLATMHGTKMRIDNVGGLTVELSPGLLSARDTRYEAYMSDLWEDILSTSINIWATNFWSDPAMSGGKHLNTVEGSLMRQEWTRAADLVHESSKARRRSPGSACGWDSIKMHQAGDDTIIFTGLDHLL--ASRKVPVESEGAEFQMACFMSLVSLLTSRAEVLRVAPYHHKTVLNAVAKAISHSATTDTSETTLTAAGLDGTGEVIQVIDSGLDQESCFFRDSDGETVELGYYFDEVAVISSTYALPLFESGGSFPVDLDRRKIVQYISLVRSDVDGENQPYGDYTSTILGQTFPWY-SAEGFATETSGGHGSHTAGTAAGSAIGST----DSCSGDDELGCLGGCLNS---SYVDQLM------SNGFFNVEVFCPKFDCNNVYADSEIYETCLSDDVEETVSANGGVAPGAKLAIFDTSVDGTTVWAELAGNGLWEASNETGSVVHTNSWGGNSDCTVDATAIAYDTFMYQNPEHLLVFSAGNDGNLSDSS--CSVGSPAVAKNVLTVGSSSSGQTRWTFTGEDGEPLESIYDTMSDIDTVSYFSSQGPTEDGRIKPEIVAPGDNVLSASSAGEDGETCELELRSGTSMAAPVVAAAAAMIRQYFKDDSFYVSDLKARELCDATTTTISSTNSSTSTSFMCSTFNASAATMKAMLINSANLMGGSSEPDGSRGFGRVHLDAGLPLGGIGDTGLFVADSSDTGVSQGTEDTYTFETVDSDTGEFRATLAWIDPPATTSSSSQLMHDLDLLVTSPSGQTYTMWS-SGETDTSNVIERVVVSADDFDDEESGTWTVSVSANDIVTESQPYSLVVTGPFGNGTRVDDSSMAAASMYRS 1040
            G S +SLP   L +VE  L TTA E F G    +E A +W+ +    K   E   A A       ++VC+   R  ETL RL+  LSP +VR +A  +  GACF  TA+  +          G    T +  +PSALK+APG+L+H ++   +S   G +LAT HG  MR  NV GL +ELSPG L A   +   ++++L +D++S SI++   N WSDP  + G+HL T  G++ ++EW+ AA LVH+ + A R SPG  C WDSI +H AG+D ++ +GLDHLL   S  +  + E  E ++ACFM LVS L+SR EV RV+P H   +LNAVA+ I  SAT   ++  LTAAGLDGTG+VIQVI                                                        QYI+L+++D        G  +ST  G    W+ S +GF  +    HG+HTAG+A G+ I +      +CSG +  GC+GGC+++   S+ D L+      S+   +++  CP   C     D E    CLSD VE+T++ +GG+A GAKL+ FD   DG  ++ +  GNGLWE   E G  +H+ S+G + +C V  T + YD FMY NPE+L+V +AGN G + D    C++ SPA+ KN L VG++SSG+TR   T  DG+  +   +  SD+DTV+ FSS GPT+DGRIKPE+VAPGD V SA S G D  +C L  +SGTSMA P+VA A+AM+RQYF D+SFY++D  AR  CD              +   C +F+ S+AT+KA+LINSANLMGGSSEPDG RGFGR+HL+ G+PL G G   LFVADSS T + + +   + F+   +   +FRATL+WIDPPA+++S  QL+HDLDL V SPSG T+ MW+ SGE D+ NV ERV+++A++    ESGT+TV VS N ++T+SQ YSLVV     NG  V  SS+A+AS+  S
Sbjct:   23 GGSANSLPYPSLGVVEGSLLTTAHESFDGHAKGFETAAQWTGDIIRSK---EEDNARA------PHLVCTEYNRGGETLSRLQELLSPEAVRSVAHSSTHGACFFVTASHAEARAMSDNPYYG---LTSISPFPSALKVAPGVLEHGVSRDQASQSPG-RLATTHGHLMRKGNVEGLNLELSPGTLPAHSPQAGMFIAELLDDLMSDSIDLHGGNVWSDPVAANGEHLTTPAGAVRKREWSMAATLVHQLAVAGRTSPGDICSWDSISIHHAGNDLLLVSGLDHLLHKESGAIGSDEEATELRVACFMGLVSFLSSRLEVSRVSPRHADRLLNAVARTIIQSATI--TKAPLTAAGLDGTGQVIQVI--------------------------------------------------------QYINLIKAD-----SVSGPASSTTSGGNRVWWVSPDGFQGDDVDDHGTHTAGSALGATIRTPAETPTTCSGTEVPGCVGGCIDADRTSWGDDLLTLMPENSSYVADIDRICPMLGC-----DEENEPWCLSDGVEQTLADHGGMAQGAKLSFFDI-YDGNMLFEQFIGNGLWEPCLEAGCKIHSLSFGADLECAVRDTDLLYDDFMYNNPENLVVVAAGNAGEIEDGRTVCTMYSPAIGKNALAVGATSSGETRVGVTSADGDRADGT-NGYSDVDTVAVFSSYGPTQDGRIKPEVVAPGDAVYSARSDGSDVHSCRLSAKSGTSMACPIVAGASAMVRQYFMDESFYLADQAARGWCD-------------DSGRQCESFSPSSATVKALLINSANLMGGSSEPDGFRGFGRIHLEMGMPLDGEGSLALFVADSSYTSLPELSSQDFVFDVDANANLDFRATLSWIDPPASSTSGVQLVHDLDLEVVSPSGATHIMWATSGEVDSRNVNERVIIAAENV---ESGTYTVRVSTNHLLTDSQNYSLVV-----NGAMVYSSSLASASVQSS 912          

BLAST of mRNA_F-serratus_M_contig864.20012.1 vs. uniprot
Match: A0A2I6U1R2_SACJA (Subtilisin (Fragment) n=1 Tax=Saccharina japonica TaxID=88149 RepID=A0A2I6U1R2_SACJA)

HSP 1 Score: 649 bits (1673), Expect = 1.670e-215
Identity = 409/951 (43.01%), Postives = 560/951 (58.89%), Query Frame = 0

Query:   71 GRSGSSLP---LALVEQLLSTTAQEDFGGRENVYEAAIEWSRNRFFHKNINENHPATAGEDYGTTYIVCSNSPR--ETLVRLESSLSPASVRKLAVGAEFGACFLATAASPDVAKAVYEELCGRGLATYVMIYPSALKLAPGLLDHSLAHTTSSYDSGAQLATMHGTKMRIDNVGGLTVELSPGLLSARDTRYEAYMSDLWEDILSTSINIWATNFWSDPAMSGGKHLNTVEGSLMRQEWTRAADLVHESSKARRRSPGSACGWDSIKMHQAGDDTIIFTGLDHLLASRKVPVES--EGAEFQMACFMSLVSLLTSRAEVLRVAPYHHKTVLNAVAKAISHSATTDTSETTLTAAGLDGTGEVIQVIDSGLDQESCFFRDSDGETVELGYYFDEVAVISSTYALPLFESGGSFPVDLDRRKIVQYISLVRSDVDGENQPYGDYTSTILGQTFPWYSAEGFATETSGGHGSHTAGTAAGSAIGST----DSCSGDDELGCLGGCLNS---SYVDQLM------SNGFFNVEVFCPKFDCNNVYADSEIYETCLSDDVEETVSANGGVAPGAKLAIFDTSVDGTTVWAELAGNGLWEASNETGSVVHTNSWGGNSDCTVDATAIAYDTFMYQNPEHLLVFSAGNDGNLSDSS--CSVGSPAVAKNVLTVGSSSSGQTRWTFTGEDGEPLESIYDTMSDIDTVSYFSSQGPTEDGRIKPEIVAPGDNVLSASSAGEDGETCELELRSGTSMAAPVVAAAAAMIRQYFKDDSFYVSDLKARELCDATTTTISSTNSSTSTSFMCSTFNASAATMKAMLINSANLMGGSSEPDGSRGFGRVHLDAGLPLGGIGDTGLFVADSSDTGVSQGTEDTYTFETVDSDTGEFRATLAWIDPPATTSSSSQLMHDLDLLVTSPSGQTYTMWS-SGETDTSNVIERVVVSADDFDDEESGTWT 998
            G S ++LP   L++VE  L TTAQ+ F G    +E A +W+      K   E   A A       ++VC+   R  +TL RL+  LSP +VR +A  +  GACF  TA+  +          G    T +  +PSALK+APG+L+H ++   +S   G +L T HG  MR  NV GL +ELSPG L A       ++++L +D++S SI++   N WSDP  + G+HL T  G++ ++EW+ AA LVH+ + A R SPG  C WDSI MH AG+D ++ +GLDHLL      + S  E  E ++ACFM LVS L++R EVLRV+P H   +LNAVA+ I  SAT   +E  LT AGLDGTG+VIQVID+GLD+ SCFF D DGE V  G+Y++ +                         +I+   S  R DV+G                                HG+H AG+A G+ I +      +CSG +  GC+GGC+++   S+ D L       S    +++  CP  DC     D      CLSDDV +T++ +GG+A GAKLA FD   DG++      GNGLWE   E G  +H+ S G + +C+V    + YD FMY NPE+L+V +AGN G+  D    C++ SPA+ KN L VG++SSG+TR T T  DG+ ++   +  +D+DTV+ FSS GPT+DGRIKPE+VAPGD + SA S G D  +C L  +SGTSMA P+VA A+AM+RQYF D+SFYV+D  AR   D              +   C +F+ S+AT+KA+LINSANLMGGSSEPDG RGFGR+HL+ G+PL G G   LFVADSS T + +     + F+   +   +FRATL+WIDPPA+++S  QL+HDLDL V SPSG T+ MW+ SGE D+ NV ERV+++A+D    ESGT+T
Sbjct:   23 GGSANTLPYPSLSVVEGSLLTTAQDSFDGHAKAFETAAQWTGEILRSK---EEDNARA------PHLVCTEYNRGGKTLPRLQELLSPGAVRPVAHSSTHGACFFVTASHAEARAMSDSPYYG---LTSISPFPSALKVAPGVLEHGVSLDQASQSPG-RLTTTHGHLMRKGNVEGLNMELSPGTLPAHSPEAGMFIAELLDDLMSDSIDLHGGNVWSDPVAANGEHLTTPAGAVRKREWSMAATLVHQLAVAGRTSPGDICSWDSISMHYAGNDLLLVSGLDHLLHKENGAIGSDEEATELRVACFMGLVSFLSNRLEVLRVSPRHADRLLNAVARTIIQSATI--TEAPLTDAGLDGTGQVIQVIDTGLDETSCFFIDDDGEEVPHGHYYEALG------------------------EIIPSSSSARDDVEG--------------------------------HGTHCAGSALGATIRTPAETPTTCSGTEVPGCVGGCIDADRTSWGDDLFTLMPEYSPYDIDIDRLCPMIDC-----DDATDPRCLSDDVGQTLADHGGMAQGAKLAFFDVFADGSS-GTHFVGNGLWEPCLEAGCKIHSMSIGADFECSVCEKDVLYDDFMYNNPENLVVVAAGNAGDTEDGRTVCTMYSPAIGKNALAVGATSSGETRITKTSADGDLIDGT-NGYADVDTVAQFSSYGPTQDGRIKPEVVAPGDAIYSARSDGSDNHSCRLSAKSGTSMACPIVAGASAMVRQYFMDESFYVADQAARGWSD-------------DSGRRCESFSPSSATVKALLINSANLMGGSSEPDGYRGFGRIHLEMGMPLDGEGSLALFVADSSYTSLPELCSQDFIFDVDANANLDFRATLSWIDPPASSASGVQLVHDLDLEVVSPSGATHIMWATSGEVDSRNVNERVIIAAEDV---ESGTYT 879          

BLAST of mRNA_F-serratus_M_contig864.20012.1 vs. uniprot
Match: D8LSW0_ECTSI (Subtilisin n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LSW0_ECTSI)

HSP 1 Score: 649 bits (1675), Expect = 5.820e-214
Identity = 426/1045 (40.77%), Postives = 584/1045 (55.89%), Query Frame = 0

Query:   79 LALVEQLLSTTAQEDFGGRENVYEAAIEWSRNRFFHKNINENHPATAGEDYGTTYIVCSN--SPRETLVRLESSLSPASVRKLAVGAEFGACFLATAASPDVAKAVYEELCGRGLATYVMIYPSALKLAPGLLDHS--------LAHTTSSYDSGAQLATMHGTKMRIDNVGGLTVELSPGLLSA--RDTRYEAYMSDLWEDILSTSINIWATNFWSDPAMSGGKHLNTVEGSLMRQEWTRAADLVHESSKARRRSPGSACGWDSIKMHQAGDDTIIFTGLDHLLASRKVPVESEGAEFQMACFMSLVSLLTSRAEVLRVAPYHHKTVLNAVAKAISHSATTDTSETTLTAAGLDGTGEVIQVIDSGLDQESCFFRDSDGETVELGYYFDEVAVISSTYALPLFESGGSFPVDLDRRKIVQYISLVRSDVDGENQPYGD---YTSTILGQTFPW----------YSAE-------------------------------------------GFATETSGGHGSHTAGTAAGS----AIGSTDSCSGDDELGCLGGCLNSSYVDQLMSNGFFNVEVFCPKFDC--NNVYADSEIYETCLSDDVEETVSANGGVAPGAKLAIFDTSVDGTTVWAELAGNGLWEASNETGSVVHTNSWGGNSDCTVDATAIAYDTFMYQNPEHLLVFSAGNDGNLSD----SSCSVGSPAVAKNVLTVGSSSSGQTRWTFTGEDGEPL-------------------------ESIYDTMSDIDTVSYFSSQGPTEDGRIKPEIVAPGDNVLSASSAGEDGETCELELRSGTSMAAPVVAAAAAMIRQYFKDDSFYVSDLKARELCDATTTTISSTNSSTSTSFMCSTFNASAATMKAMLINSANLMGGSSEPDGSRGFGRVHLDAGLPLGGIGDTGLFVADSSDTGVSQGTEDTYTFETVDSDTG-EFRATLAWIDPPATTSSSSQLMHDLDLLVTSPSGQTYTMWSSGETDTSNVIERVVVSADDFDDEESGTWTVSVSANDIVTESQPYSLVVTG 1019
            L  ++ + +++A +DF   ++ Y AA  W  +R  +          A E     Y+ C++    R  L  LE++ + ++V +++     G CF+ TA  P  A AV        L +   + PS LKLA  LLDH          A      D  A L + +G  M +DNV GL+V LSPG+L+A  R++  EA++ D   D+ S S+N+   +FWSD      ++  T       +EW+RAA +V   +    R+ G  CG+  +K+    DD ++  G++HLL  ++ P E+     + AC+M L+S L ++ EVLRV+P     + NAVA A+  SATT  +ET L  AGLDGTGEVIQV+DSGLD+ SCFF D DG  VE G+ FD V V        +     SFP D+ RRKIVQYI L ++   G      D   Y S  LG  + +          YS E                                           GF  +T GGHG+ TAG+AAG+    +I   ++C GD+  GC GGC+++S VD ++ +  F+++VFCP + C  N     SE    CLSDD  E +  +GGVAPGA++++FD S  GT ++A  AGN LW ++ ETG+ +HTNSWGG + C +      YDTFMY+NPEHLL+F+AGNDG   D     +C+VGSPA+ KN L VG++SSG    T TG+DG  +                          S     + +DTV++FSSQGPT DGRIKP++VAPGD V SASS G DG +C+L    GTS + P+VA AAA++RQYFKD SF+  D+ +R  C           +  S+S  CS F  SAAT+KAM+INSA+LMGGSSEP+G RGFGRVHL+AG+P+ G+G+TGL V +++   ++ G  +     TVD D G E RATLAW+DPP T  S+ QL HDLDL V +PSG  YTMW SG  DT NV+ERV+V  +     ESG W+V VSA  ++T+ Q YSLVVTG
Sbjct:   14 LGKIQAITASSAFDDFARSQSAYSAASSWYNDRLSYD---------AAEGSTAPYVACADYKQGRHALTSLETAFTKSAVHRVSNTEADGTCFIVTAL-PSSATAVIRAPEDFDLLSAAPLLPS-LKLATSLLDHGPDTSPRLGAADDFDLSDHSAPLRSTYGEGMTLDNVHGLSVRLSPGVLTAGGRESFAEAFLRDWHADLTSESVNMRRASFWSDSDADRSEYDVTRV-----REWSRAAAVVDGLASKSARTVGEICGFGKLKLRHVADDLVLVEGMNHLLPGKRFPSET-----RTACYMGLLSQLAAKPEVLRVSPLQSARLHNAVANALVQSATT--TETPLLDAGLDGTGEVIQVVDSGLDETSCFFADDDGLQVEHGFLFDGVRVEDDGNVSTVL-GNYSFPFDMSRRKIVQYIQLFKTPDQGSTSSGQDSFTYVSDYLGDPYDYDDSEYISYDDYSGEYYSDTWSEDSFCAGVLDDLSYHFSYLFDDTSYSSSSTSPSFTGGFHKDTVGGHGTWTAGSAAGAISAQSILEEEACYGDELPGCAGGCISASDVDAMLDDSTFDLDVFCPMYTCDGNTDIPSSE----CLSDDPVENLHQHGGVAPGAQISVFDVSYVGTEIYASFAGNDLWYSAMETGAKIHTNSWGGATLCQLTEDEYLYDTFMYENPEHLLIFAAGNDGGYEDIPNREACTVGSPALGKNSLAVGATSSGPFPGTRTGDDGNLVYEELGLTDYSPEGYPWICLTPFLGTPSASTEPAGVDTVAFFSSQGPTLDGRIKPDVVAPGDQVASASSDGTDGHSCKLVASMGTSASCPLVAGAAALVRQYFKDPSFFAKDVVSRGGC-----------AEHSSSCQCSEFAPSAATVKAMIINSAHLMGGSSEPNGVRGFGRVHLEAGMPMDGVGETGLSVVEAA---IADG--EITELVTVDGDAGVELRATLAWLDPPTTALSAKQLQHDLDLHVIAPSGAKYTMWESGVDDTVNVVERVIVPVESM---ESGVWSVVVSAKALLTDEQLYSLVVTG 1011          

BLAST of mRNA_F-serratus_M_contig864.20012.1 vs. uniprot
Match: D8LQI1_ECTSI (Subtilisin n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LQI1_ECTSI)

HSP 1 Score: 632 bits (1630), Expect = 1.940e-207
Identity = 416/977 (42.58%), Postives = 566/977 (57.93%), Query Frame = 0

Query:   79 LALVEQLLSTTAQEDFGGRENVYEAAIEWSRNRFFHKNINENHPATAGEDYGTT--YIVC--SNSPRETLVRLESSLSPASVRKLAVGAEFGACFLATAASPDVAK-AVYEELCGRGLATYVMIYPSALKLAPGLLDHSL---------AHTTSSYDSGAQLATMHGTKMRIDNVGGLTVELSPGLL---SARDTRYEAYMSDLWEDILSTSINIWATNFWSDPAMSGGKHLNTVEGSLMRQEWTRAADLVHESSKARRRSPGSACGWDSIKMHQAGDDTIIFTGLDHLLAS-RKVP--VESEGAEFQMACFMSLVSLLTSRAEVLRVAPYHHKTVLNAVAKAISHSATTDTSETTLTAAGLDGTGEVIQVIDSGLDQESCFFRDSDGETVELGYYFDEVA-----VISSTYALPLFES---GGSFPVDLDRRKIVQYISLVRSDVDGENQPYGDYTSTILGQTFPWYSAEGFATETSGGHGSHTAGTAAGSAIGSTD---SCS-GDDELGCLGGCLNSSYVDQLMSNGFFNVEVFCPKFDCNNVYADSEIYETCLSDDVEETVSANGGVAPGAKLAIFDTSVDGTTVWAELAGNGLWEASNETGSVVHTNSWGGNSDCTVDATAIAYDTFMYQNPEHLLVFSAGNDGNLSD-SSCSVGSPAVAKNVLTVGSSSSGQTRWTFTGEDGEPLESIYDTMSDIDTVSYFSSQGPTEDGRIKPEIVAPGDNVLSASSAGEDGETCELELRSGTSMAAPVVAAAAAMIRQYFKDDSFYVSDLKARELCDATTTTISSTNSSTSTSFMCSTFNASAATMKAMLINSANLMGGSSEPDGSRGFGRVHLDAGLPLGGIGDTGLFVADSSDTGVSQGTEDTYTFETVDSDTG-EFRATLAWIDPPATTSSSSQLMHDLDLLVTSPSGQTYTMWSSGETDTSNVIERVVVSADDFDD-EESGTWTVSVSANDI-VTESQPYSLVVTG 1019
            L   EQ L TTA  DF   E  +  A  W                 AG+  G +  Y+ C      R    +LE  L   +V+ ++  A  GACF+ TA+  + A+ + +E+    G       +P+ALK+APGLLDH           A TT+S  S  +L+T HG  M++D+  GL VELSPG L   S  D +   ++ +L + + S S+++ A   WSD  M GG HL +  G+L  +EW+RAA LV+E S++ + +P   CGW+ + +H A +D ++  GLDHLL   R+V    E E AE +MAC+M LVS L SR EVL+VA ++ K  LNA A+A   SA+ D+  T LT AGLDG+GEVIQ+ID+G+++ +CF    DG  VE GYYFD VA       SS+ +  +F++   GG F  D DRRK++QYI L+  D        GD +S+ +    P+ SA+ F ++   GHG+H A T AGS I S     SC+ G + +GC GGC+  S      ++   ++   CP F C+         + CL DD  +T+S +GG+A GAK+A  D    G   + +LAGNGLWE   + G  +H+NS+G +  C + A  + YD +MY+N EHLL+FSAGN G  +D  +C++ SP +AKNVLTVGS+SSG TR + T  DG       D +S ID VS FS  G TEDGRIKPE+VAPGD + SAS  G D  +CEL   +GTS +  V + AAAM+RQYF+D SFY +D+++R  CD                F C +F+  A T+KAMLINSAN+MGG +EPD  RGFGR+HL+AG+PLGG GD  LFVADSSD  V    +  + F  VD D G E RATL+W DP A++ S  QL++DLDL V +P G  Y MW     DTSNV ERV+V A+       +  W V V   D  V ++  +SLVVTG
Sbjct:   27 LGFFEQTLLTTAGADFDS-EAAFAFASAWQ----------------AGDRQGRSSPYLSCVEHGEGRHAYRKLEGFLGARAVKPVSSTATHGACFMVTASPAEAAQLSSWEDFTTFGA------FPAALKIAPGLLDHGSCDGPAAARDAATTTSDCSAGRLSTTHGASMQVDSAHGLMVELSPGTLPSHSGGDAKAHEFIQELLKSLSSPSLDLHAITCWSDLVMKGGDHLFSAAGALRGREWSRAATLVNERSESEQETPSQVCGWEQVVVHHAANDVLLVAGLDHLLYDGREVSGASEEEAAELRMACYMGLVSFLASRHEVLKVAGWNRKVNLNAAARATIQSASVDS--TPLTDAGLDGSGEVIQIIDAGVNETTCFLEHGDGTEVEHGYYFDFVASSNPLFTSSSESRSMFKAEFTGGDFSYDSDRRKVIQYIDLINPDT-------GDGSSSGM----PFVSADSFVSDDDLGHGTHAATTVAGSTISSPAELVSCADGSEVIGCAGGCITESSTGSSEADYGEDINRLCPAFGCSG--------DQCLPDDTADTLSEHGGMAQGAKIAAIDVFY-GDYSYGDLAGNGLWETCADAGCKIHSNSYGVDLRCQLTALDVLYDEYMYENQEHLLLFSAGNTGWTTDRDTCTIDSPGIAKNVLTVGSTSSGVTRLSTTDLDGRGYADG-DELSTIDVVSEFSGYGFTEDGRIKPEVVAPGDMIYSASGNGGDS-SCELIAYAGTSFSTAVASGAAAMVRQYFRDSSFYSADVESRGFCDQ--------------GFTCDSFSPMATTVKAMLINSANVMGGGNEPDTERGFGRIHLEAGMPLGGAGDMALFVADSSDHEVYSDEKLKFYFN-VDPDAGMELRATLSWNDPAASSLSGDQLVNDLDLRVYAPDGTRYCMWDDNCWDTSNVNERVIVPAEVVAQFSATDEWEVRVRGRDFTVGDTVSFSLVVTG 941          

BLAST of mRNA_F-serratus_M_contig864.20012.1 vs. uniprot
Match: A0A6H5JKA8_9PHAE (Subtilisin n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JKA8_9PHAE)

HSP 1 Score: 596 bits (1536), Expect = 8.310e-192
Identity = 424/1097 (38.65%), Postives = 592/1097 (53.97%), Query Frame = 0

Query:   78 PLALVEQLLSTTAQEDFGGRENVYEAAIEWSRNRFFHKNINENHPATAGEDYGTTYIVCSNSPRE---TLVRLESSLSPASV--RKLAVGAEFGACFLATAASPDVAKAVYEELCGRGLATYVMIYPSALKLAPGLLDHSLAHTTSSYDSGAQLATMHGTKMR---------------------------------------IDNVGG-------LTVELSPGLLSARDTRYEA---------YMSDLW-EDILSTSINIWATNFWSD----------PAMSGGKHLNTVEGSLMRQEWTRAADLVHESSKARRRSP-GSACGWDSIKMHQAGDDTIIFTGLDHLLASRKVPVESEGAE---FQMACFMSLVSLLTSRAEVLRVAPYHHKTVLNAVAKAISHSATTDTSETTLTAAGLDGTGEVIQVIDSGLDQESCFFRDSDG-ETVELGYYFDEVAVISSTYA-----------LPLFESGGSFPVDLDRRK---------------IVQYISLVRSDV---DGENQP--------YGDYTSTILGQTFPWYSAEGFATETSGGHGSHTAGTAAGSAIGS---------TDSCSGDDELGCLGGCLN-----------SSYVDQLMSNGFFNVEV--FCPKFDCNNVYADSEIYETCLSDDVEETVSANGGVAPGAKLAIFDTSVDGTTVWAELAGNGLWEASNETGSVVHTNSWGGNSDCTVDATAIAYDTFMYQNPEHLLVFSAGNDGNLSDSSCSVGSPAVAKNVLTVGSSSSGQTRWTFTGEDGEPLESIYDTMSDIDTVSYFSSQGPTEDGRIKPEIVAPGDNVLSASSAGEDGETCELELRSGTSMAAPVVAAAAAMIRQYFKDDSFYVSDLKARELCDATTTTISSTNSSTSTSFMCSTFNASAATMKAMLINSANLMGGSSEPDGSRGFGRVHLDAGLPLGGIGDTGLFVADSSDTGVSQGTEDTYTFETVDSDTG-EFRATLAWIDPPATTSSSSQLMHDLDLLVTSPSGQTYTMWSSGETDTSNVIERVVVSADDF------------DDEESGTWTVSVSANDIV-TESQPYSLVVTGPFGNGT 1025
            PL+++   L TTA   F   +  ++AA+EW             H A   +     Y+ C++   +   +   L   L   ++  R +   AE G C +ATA +P  A+++++   G+ + +    +PSALKLAPGLLDH           G +L   HG ++R                                        DN+GG       LTVEL+PG+L+A + R  +          ++D W E+++S S+++   +FWS            A   G       G ++ +EWTRAAD++            G  C WD + +    DD +   G+DHLL   +    +EG E    ++ACFM+L+S L SR EVLRV+P     +LNA A     SA+   +E+ +T AGLDGTGEVIQ++D+GLD  SCFF D DG E V  GYYF+E+ V +S++            +    +GG F  DL RRK               I +  +L   DV    GEN          YG+  +T  G+ +PW SA  FA + +GGHG+HTAG+AAG+ + S         T+ C    EL C+GGC++           +++  Q    G+  +++   CP+ DC+++       E CL DD  ET+  +GGVA GA+LAIFD   D T V  ++AGNG+WE + E GS + +NSWG  + C   +  + +D FMY+N + LL+F+AGNDG+   S C++G+PA+AKNVL VG+S+SG  R +FT EDG+P +     + DID ++YFSS GPT D RIKPE+VAPGD + SA+ +G+   TC L   +GTSM+ P+VA AAA++RQYFK+++FY  D++ R  CD               SF C  F  SAAT+KAMLINSANLMGGS+EPDG RGFGRVHL+AG+PL G GD  LFVADS+ T  ++ T   Y+F  VD+  G + RATL+WIDPPATT SS QL+HDLDL V SP+G  Y+MW  G  D+ N  ERV+V A +             D E  GTWTV V +  +   E+Q YSLVVTG  G  T
Sbjct:   31 PLSVIRGALLTTAGVTFR-HQGEFKAAVEW-------------HEARLDDARPAPYLACASPDSDRGGSGATLSHVLGAGAIGGRVVVSSAEHGVCVVATA-TPGQAESIFQ---GQQVFSSWAAFPSALKLAPGLLDHPHDDRDGGGGDG-RLRMTHGERLRRPKDRKGXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXDNIGGGPSGVTGLTVELAPGVLAATNRRGSSGGGWYASSGELTDEWREELMSQSLDVHDKSFWSSGWSAKLPDEAAAEGAGDSSGGGPGEVLAREWTRAADVLRGLGGTEGGPTIGDVCSWDGLVVVHTDDDLLTMKGMDHLLPEGRGAGGNEGPEADELKVACFMALLSFLASRPEVLRVSPARAIKLLNAAAYHNVQSASL--TESPMTDAGLDGTGEVIQIVDTGLDVNSCFFVDEDGGEGVAHGYYFEEIGVEASSFGSWFTSWDFNVQIARVFTGGDFNFDLSRRKGLVVDAAHPSCDSPNISRRFAL--GDVRAWSGENPACTANNSTNYGESWATSAGEQYPWISAAEFAEDDAGGHGTHTAGSAAGAVVSSPSYSQTGDFTEVCEEGRELSCVGGCIDVDDPFGEDDLITTWDYQYFWAGYEAIDLDRLCPRLDCDDLPE-----EYCLGDDEAETLQQHGGVARGAQLAIFDIINDYTGVGIDMAGNGMWEPAMEAGSKLSSNSWGIYAYCDYLSLDVLFDDFMYRNQDQLLLFAAGNDGD-DWSGCTMGTPALAKNVLAVGASTSGPGRLSFTDEDGDPTDGD-SRVQDIDAMAYFSSFGPTLDNRIKPEVVAPGDMIYSAAGSGDGNYTCRLWQYAGTSMSTPIVAGAAALVRQYFKNETFYAHDVEERGFCD---------------SFGCEPFAPSAATVKAMLINSANLMGGSAEPDGLRGFGRVHLEAGMPLAGEGDNALFVADSAVTSTAELTLTEYSFA-VDAAAGIDLRATLSWIDPPATTFSSVQLVHDLDLSVYSPTGLRYSMWRDGTVDSLNNNERVIVPASNILDQTSSDATTAEDGESDGTWTVRVWSKRLAGNETQSYSLVVTGAIGPPT 1081          

BLAST of mRNA_F-serratus_M_contig864.20012.1 vs. uniprot
Match: D8LU20_ECTSI (Subtilisin n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LU20_ECTSI)

HSP 1 Score: 582 bits (1499), Expect = 2.690e-188
Identity = 400/1043 (38.35%), Postives = 555/1043 (53.21%), Query Frame = 0

Query:   79 LALVEQLLSTTAQEDFGGRENVYEAAIEWSRNRFFHKNINENHPATAGEDYGTTYIVCSN--SPRETLVRLESSLSPASVRKLAVGAEFGACFLATAASPDVAKAVYEELCGRGLATYVMIYPSALKLAPGLLDHSLAHTTSSYDSGAQLATMHGTKMRIDNVGGLTVELSPGLLSARDTRYEAYMSDLWEDILSTSINIWATNFWSDPAMSGGKHLNTVEGSLMRQEWTRAADLVHESSKARRRSPGSACGWDSIKMHQAGDDTIIFTGLDHLLASRKVPVESEGAEFQMACFMSLVSLLTSRAEVLRVAPYHHKTVLNAVAKAISHSATTDTSETTLTAAGLDGTGEVIQVIDSGLDQESCFFRDSDGETVELGYYFDEVAVISSTYALPLFESGGSFPVDLDRRKIVQYISLVRS-----------------------------DVDGENQPYGDYTSTILGQTFPWYS---------------------------------AEGFATETSGGHGSHTAGTAAGSAIGST----DSCSGDDELGCLGGCLNSSYVDQLMSNGFFNVEVFCPKFDC--NNVYADSEIYETCLSDDVEETVSANGGVAPGAKLAIFDTSVDGTTVWAELAGNGLWEASNETGSVVHTNSWGGNSDCTVDATAIAYDTFMYQNPEHLLVFSAGNDGNLSD----SSCSVGSPAVAKNVLTVGSSSSGQTRWTFTGEDGEPLESIYDTMSD--------------------------IDTVSYFSSQGPTEDGRIKPEIVAPGDNVLSASSAGEDGETCELELRSGTSMAAPVVAAAAAMIRQYFKDDSFYVSDLKARELCDATTTTISSTNSSTSTSFMCSTFNASAATMKAMLINSANLMGGSSEPDGSRGFGRVHLDAGLPLGGIGDTGLFVADSSDTGVSQGTEDTYT-FETVDSDTG-EFRATLAWIDPPATTSSSSQLMHDLDLLVTSPSGQTYTMWSSGETDTSNVIERVVVSADDFDDEESGTWTVSVSANDIVTESQPYSLVVTG 1019
            L  ++ + +++A +DF   ++ Y AA +W   R  H          A E     YI C++    R  +  LE++ + ++V +++     G+CF+ TA  P  A A+        L +     PS LKLA  LLDH                                 + SP + +A D      +SDL   + ST                 G+  +  + + +R EW+RAA +V   +  R ++ G  CG+  + +    DD ++  G++HLL  +K P E+     + AC+M L+S L ++ EVLRV+P    ++ NAVA A+  SATT  +ET L  AGLDGTGEVIQV+D+GLD+ SCFF D DG  VE G+ F  V V        +     SFP D+ RRKIVQYI + ++                             D D E   Y DY+      T+   S                                   GF  +  GGHG+ TAG AAG+    +    ++C GD+  GC GGC+++S VD ++ NG F+++VFCP ++C  N     SE    CLSDD  E +  +GGVAPGA++++ D S  G+  +A  AGN LW ++ ETG+ +H+NSWG +  C +      YD+FMY+NPEHLL+F+AGN G   D      C++GSPA+ KN+L VG++SSG +R T TGEDG  +   +  M+D                          +DTV++FSSQGPT DGRIKP++VAPGD V SASS G DG +C L    GTS + P+VA AAA++RQYFKD SF+  D+ +R  C           +  S+S  CS F  SAAT+KAM+INSA+LMGGSSEP+G RGFGRVHL+AG+P+ G+G+TGL V +++      G++D  T   TVD D G E RATLAW+DPP T  S+ QL HDLDL V +PSG  YTMW SG  DT NV+ERV+V  +     ESG W+V VSA  ++T+ Q YSLVVTG
Sbjct:   25 LGKIQTITASSAFDDFARSQSAYSAASKWHTKRLAH----------APEGSRAPYIACADYKQGRHAMTSLETAFTESAVHRVSNTETDGSCFIVTAL-PSSATAMISAPEDFALLSAAPFLPS-LKLATRLLDHG-------------------------------PDTSPHVGAADDFN----LSDLSAPLRSTY----------------GEDRSQYDVTRVR-EWSRAAAVVDSLASKRAQTFGEICGFGKLSLRHVADDLLLVEGMNHLLPGKKAPSEA-----RTACYMGLLSQLAAKPEVLRVSPLQSASLYNAVANALVQSATT--TETPLLDAGLDGTGEVIQVVDTGLDETSCFFADDDGLQVEHGFLFGGVRVEGDGNVSTVL-GNYSFPFDMSRRKIVQYIQMFKTPDQGSTSSGQNSFTYASDYIRDYLGDAFDDDDSEYNSYDDYSGEYSSDTWSEESFCAQVLDDLSYHFSSLFDDTSYSPSSASSSISSTGGFDKDIVGGHGTWTAGCAAGAISAQSSLQEEACYGDELPGCAGGCISASDVDAMLDNGSFDLDVFCPMYNCDGNTDIPSSE----CLSDDPVENLHQHGGVAPGAQISMLDASYTGSDSFALFAGNMLWYSAMETGAKIHSNSWGYDMLCQLTENEYLYDSFMYENPEHLLIFAAGNHGGNEDIPNHEGCTIGSPALGKNILAVGATSSGPSRGTRTGEDGNLVYEEFG-MTDYSPEGYPWICLSPSLGTPSASMEPAGVDTVAFFSSQGPTLDGRIKPDVVAPGDQVASASSDGTDGHSCRLLANMGTSASCPLVAGAAALVRQYFKDPSFFAKDVTSRGGC-----------AEHSSSSQCSEFAPSAATVKAMIINSAHLMGGSSEPNGLRGFGRVHLEAGMPMDGVGETGLLVVEAT-----IGSDDEITELVTVDGDAGAELRATLAWLDPPTTALSAKQLQHDLDLHVIAPSGAKYTMWESGADDTVNVVERVIVPVESM---ESGVWSVVVSAKGLLTDEQSYSLVVTG 971          

BLAST of mRNA_F-serratus_M_contig864.20012.1 vs. uniprot
Match: D8LNG4_ECTSI (Subtilisin n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LNG4_ECTSI)

HSP 1 Score: 520 bits (1339), Expect = 2.240e-167
Identity = 320/702 (45.58%), Postives = 436/702 (62.11%), Query Frame = 0

Query:  371 MSLVSLLTSRAEVLRVAPYHHKTVLNAVAKAISHSATTDTSETTLTAAGLDGTGEVIQVIDSGLDQESCFF-RDSDGETVELGYYFDEVAV----ISSTYALPLFE---------------------------SGGSFPVDLDRRKIVQYISLVRSDVDGENQPYGDYTSTILGQTFPWYSAEGFATETSGGHGSHTAGTAAGSAIGSTD---SCSGDDELGCLGGCLNSSYVDQLMSNGFFNVEV-----FCPKF---DCNNVYADSEIYETCLSDDVEETVSANGGVAPGAKLAIFDTSVDGTTVWAELAGNGLWEASNETGSVVHTNSWGGNSDCTVDATAIAYDTFMYQNPEHLLVFSAGNDGNLSDSSCSVGSPAVAKNVLTVGSSSSGQTRWTFTGEDGEPLESIYDTMSDIDTVSYFSSQGPTEDGRIKPEIVAPGDNVLSASSAGE-DGETCELELRSGTSMAAPVVAAAAAMIRQYFKDDSFYVSDLKARELCDATTTTISSTNSSTSTSFMCSTFNASAATMKAMLINSANLMGGSSEPDGSRGFGRVHLDAGLPLGGIGDTGLFVADSSDTGVSQGTEDTYTFETVDSDTG-EFRATLAWIDPPATTSSSSQLMHDLDLLVTSPSGQTYTMWSSGETDTSNVIERVVVSADDFDDEESGTWTVSVSANDIVTESQPYSLVVTGPFGNGTRV 1027
            M LVS L S+ EVLRVAP   + +LNA A+A   SAT   ++T LT AGLDGTG+V+QVIDSG+D+ SCFF  D  G+ V  G+YF+E  +     SS+++ P                               GG F +  DRRKI+QYI+L++SD D E   +GD  +T LGQ +PW SA+ F  +   GHG+H AG+ AG+ + +     SC+  + + C+G C++    + +       VE+      CP F   DC+ +  D      CLSDDV ET++ NGG+A GAKLAIFD   D T   +   G GLWE   +    +H+NS+GG+ +C++ +  + YD +M  NPE+LL+F+AGNDG L  S+C++GSPA+AKNVL VG+S+SG TR + T   G            IDTV+ FSS GPT D RIKPE++APGD + SA+S G  D  +C L    GTSM+ P+VA A+AMIRQYF +++F+ +D+ AR  CD                  C  F+ S+AT+KA++INSANLMG SSEP+GSRGFGR+HL+AG+PLGG G   LFV+DS+ T + + T   Y F+ V++  G + R TL+W+DPPATT S+ QL+HDLDL+V SP+G  YTMW+SG TD+SNV ERV+V ADD    E+GTW+V + A+++ T+ Q YSLVV G    GT V
Sbjct:    1 MGLVSFLASKPEVLRVAPRQSQALLNAAARANIQSATV--TDTPLTDAGLDGTGQVVQVIDSGVDESSCFFAHDESGDHVPHGHYFEEWGINPSLTSSSFSSPXXXXXXXXXXXPWATADHHTGSYYQIAEEFDGGDFTIYPDRRKIIQYINLIKSD-DTEGS-HGDSFTTSLGQQYPWLSADHFEYDDEAGHGTHVAGSVAGATLNTPAELISCNDTETMSCVGACVDDDEANDVDDLATPYVEIADLDRLCPLFSDYDCDGLETDK-----CLSDDVAETLTNNGGMAQGAKLAIFDAFYD-TFGLSPFVGTGLWEPCADADCKIHSNSYGGDFECSLGSNDVLYDEWMVSNPENLLIFAAGNDGELRRSTCTIGSPAIAKNVLAVGASTSGDTRLSATPGSG------------IDTVAVFSSYGPTSDSRIKPEVLAPGDAIYSAASDGSVDTHSCRLWAYVGTSMSTPIVAGASAMIRQYFMNETFFATDMTARGFCDEWAG--------------CEAFSPSSATLKALMINSANLMGESSEPNGSRGFGRIHLEAGMPLGGEGSLALFVSDSNYTSIPELTRQEYNFD-VNATAGLDLRVTLSWLDPPATTFSAVQLVHDLDLVVISPNGTRYTMWASGVTDSSNVNERVIVDADDV---ETGTWSVWIWADNLTTDVQSYSLVVNGAISPGTGV 662