mRNA_F-serratus_M_contig646.17403.1 (mRNA) Fucus serratus male

BLAST of mRNA_F-serratus_M_contig646.17403.1 vs. uniprot
Match: A0A6H5K229_9PHAE (Subtilisin n=2 Tax=Ectocarpus TaxID=2879 RepID=A0A6H5K229_9PHAE)

HSP 1 Score: 1087 bits (2811), Expect = 0.000e+0
Identity = 573/944 (60.70%), Postives = 692/944 (73.31%), Query Frame = 1

Query:    1 PSLSLVERALRTTAIHDFDGHDPAYQAAVHWDRQRLRDNFSHQRAPYLACAEYGEGREAMKSLKEFLSPEAVRPASNTVETGACFIVTASNEQATSISAHAEQFWLLSVGPVPSALKLAPGLLDHNND-------GNSSERLATTHGKRMRMANVEGLNIELAPGTLRMHAPEVISFAKSLLEDLTSKSLDLHVGNVWSDPVMTGADHHLTNGGAWRAREWIRAADVVEELTEAVDTSPSDICSWESTAIRQASDNVLMVSGVDHLLTSGRGAGGQGEE-AVELHMACFMGLVSFLASRPEVLRVAPRHKTRLLNAAARANIQTATVTSTPLTDAGLDGTGEVIQVVDTGLDETSCFFIDDDGEEIEHGHYFEELAWVATYYLTPYYSFYRYTTDTAVHEVFNGGDFVYDTSRRKIIQYINLVKSDSEPSSHGEYYTTEQGAQITWLDAADFEQDDAAGHGTHTAGSAAGAMLNTASETVTCSSSDVLGCVGACISDSTTYEYDDLLTYVDQFYSADLDRLCPSFDCGGYDEEVCLSDDVEQTLADHGGMAQGAKLAIFDIFYEESGFGSSAGNGVWEACLEAGCKVHSNSYGGDLICEMGEVDVEYDDFMYNNPENLLVFAAGNEGDIDDGRDVCTIISPAIAKNVLAIGSTSSGKTRLTTTAADGEGSGDFNGGADIDTVSFFSSYGPTLDGRIKPELVAPGDMIYSAASDATEVYTCRLWAFQGTSMSCPVVAGAAAMVRQYFVDDTFYAADVTARGLCSDGFVCEGFSPSSATVKALLINSANLMGESSDPDYFRGFGRIHMEAGMPLGGEGDLVLFVADSANTNIAGQATQQYLFDVDSDAGLDLRATISWIDPAATTFSGKQLIHDLDLTVIAPSGATHRMWSTGIADANNVNERVIVDAVDVANGTWAISVRANELTTDVQSYSLVVNGAISPATGD 2808
            PSL LVE  L TTA  DF+GH  A++AA  W+ +RLR +    RAP+L CAEYG GRE    LK+FLSP+AVR  S++ + GACF+ TAS+ QA++I    EQF L S  P PSALKLAPGL+DH+          +  +RL   HG  MRM+NVEGLN++L P TL   +    SF   LL DL S S+DLH  N WSDPVM G +H  ++GGA RAR+W +AA VV EL+EA  TSP DICSW+  A+ QA D+ L+V+G+DHLL SGRGAGG  EE + ELH+ACFMGLVSF A RPEVLRV+PRH  R+LN AAR  IQ+A+ T TPLTDAGLDGTGEVIQVVDTGLDETSCFFIDD GEE++HG++FEEL   +T            ++    + VF  GDF Y   RRK+IQYI LV  DS+  +    ++T QG    +L   +F QDD+AGHGTHTAGSAAGA LN  +ET+TCS  + LGCVG CI +S T   DDL++Y  Q    DLDRLCP + C G   + CLSD+V++TL  +GGMAQGAKL+IFD+F+E  G     GNGVWE CLEAGCKVHS S GGD +C  GE ++ YDDFMY NPENLL+FAAGN+G+ +  R VCT+ SPAI KNVLA+G+++SG TR+ +T           GGA ID V+ FSSYG T DGRIKPE+VAPGDM+YSAASD T+ ++CRLWA+QGTSMSCP+VAGAAAMVRQYFVD+TFY ADVTARG CSDGF+CEGFSPS+ATVKALLINSANLMGESS PD +RGFGRIH+E GMPL GEGDL LFVAD+ +T+IA    Q+YLFDVD+DAGL+LRAT+SWID  AT+ S  QL++DLDL V++PSG  +RMW + + D  NVNERVIVDA DV  GTW + V +N L TD QSYSLVVNGAIS  TG+
Sbjct:   32 PSLELVENGLATTAKDDFNGHARAFEAAATWNGERLRRSQDGTRAPHLCCAEYGHGRETFSRLKQFLSPDAVRVVSHSEDHGACFLATASHAQASAIMEDQEQFGLESFSPFPSALKLAPGLVDHSESREVEGEPSSGQDRLGARHGGTMRMSNVEGLNVKLTPDTLPARSAGAESFIIDLLGDLMSASVDLHSTNFWSDPVMAGGEHLSSHGGAVRARDWSKAASVVHELSEAAGTSPGDICSWDEVALIQAGDDSLLVTGLDHLLFSGRGAGGSHEEESTELHVACFMGLVSFFAGRPEVLRVSPRHTKRVLNEAARGIIQSASATDTPLTDAGLDGTGEVIQVVDTGLDETSCFFIDDGGEEVDHGYFFEELFVGSTG-----------SSQLIYYPVFEAGDFTYTEERRKVIQYIELVNPDSDYVA----FSTTQGGVYPFLPPDEFGQDDSAGHGTHTAGSAAGATLNDPAETLTCSGDETLGCVGGCIDESETT--DDLVSYAIQIALPDLDRLCPMYGCDG-STDPCLSDEVDETLTKNGGMAQGAKLSIFDVFFEVFGLIEVLGNGVWEPCLEAGCKVHSLSLGGDTLCTPGESEILYDDFMYENPENLLIFAAGNDGETE--RSVCTMASPAIGKNVLAVGASTSGDTRVIST----------EGGAGIDMVADFSSYGFTTDGRIKPEVVAPGDMVYSAASDGTDSHSCRLWAYQGTSMSCPIVAGAAAMVRQYFVDETFYTADVTARGFCSDGFICEGFSPSAATVKALLINSANLMGESSQPDAYRGFGRIHLEQGMPLEGEGDLALFVADALDTSIAEDTVQEYLFDVDADAGLELRATLSWIDYPATSTSAMQLVNDLDLVVVSPSGTVYRMWGSDVTDNRNVNERVIVDADDVETGTWTVLVYSNSLVTDSQSYSLVVNGAISAGTGE 945          

BLAST of mRNA_F-serratus_M_contig646.17403.1 vs. uniprot
Match: A0A6H5JQT4_9PHAE (Subtilisin n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JQT4_9PHAE)

HSP 1 Score: 1026 bits (2652), Expect = 0.000e+0
Identity = 555/968 (57.33%), Postives = 677/968 (69.94%), Query Frame = 1

Query:    1 PSLSLVERALRTTAIHDFDGHDPAYQAAVHWDRQRLRDNFSHQRAPYLACAEYGEGREAMKSLKEFLSPEAVRPASNTVETGACFIVTASNEQATSISAHAEQFWLLSVGPVPSALKLAPGLLDHNNDGNSS-------ERLATTHGKRMRMANVEGLNIELAPGTLRMHAPEVISFAKSLLEDLTSKSLDLHVGNVWSDPVMTGADHHLTNGGAWRAREWIRAADVVEELTEAVDTSPSDICSWESTAIRQASDNVLMVSGVDHLLTSGRGAGGQ--GEEAVELHMACFMGLVSFLASRPEVLRVAPRHKTRLLNAAARANIQTATVTSTPLTDAGLDGTGEVIQVVDTGLDETSCFFI-DDDGEEIEHGHYFEELAWVATYYLTPYYSFYRYTTDT-------------------AVHEVFNGGDFVYDTSRRKIIQYINLVKSDSEPSSHGEYYTTEQGAQITWLDAADFEQDDAAGHGTHTAGSAAGAMLNTASETVTCSSSDVLGCVGACISDSTTYEYDDLLTYVDQFYSADLDRLCPSF---DCGGYDEEVCLSDDVEQTLADHGGMAQGAKLAIFDIFYEESGFGSSAGNGVWEACLEAGCKVHSNSYGGDLICEMGEVDVEYDDFMYNNPENLLVFAAGNEGDIDDGRDVCTIISPAIAKNVLAIGSTSSGKTRLTTTAADGEGSGDFNGGADIDTVSFFSSYGPTLDGRIKPELVAPGDMIYSAASDAT-EVYTCRLWAFQGTSMSCPVVAGAAAMVRQYFVDDTFYAADVTARGLCSDGFVCEGFSPSSATVKALLINSANLMGESSDPDYFRGFGRIHMEAGMPLGGEGDLVLFVADSANTNIAGQATQQYLFDVDSDAGLDLRATISWIDPAATTFSGKQLIHDLDLTVIAPSGATHRMWSTGIADANNVNERVIVDAVDVANGTWAISVRANELTTDVQSYSLVVNGAISPATG 2805
            PSL LVE AL TTA  DF+GH  A++AA  W+ +RLR      RAP+L CAEYG GRE    LK+FLSPEAVR  S++ + GACF  +AS+ QA++I    EQF L S  P PSALKLAPGL+DH+   +         +RL+  HG RMRM+NVEGL+IEL PGTL   + E  +F    L DL   S+DLH  N WSD  M G +     GGA RAR+W +AA VV EL+EA  TSP DICSW   ++  A  +VL+VSG+DHLL SGRGAGG+  G E+ ELH+ACFMGLVSFLAS+PEVLRVAPR    LLNA+ARANIQ+ATVT TPLTDAGLDGTG+V+QV+D+G+DE+SCFF  D+ G+ + HGHYFEE  W     LT   SF   T+D                     + E F+GGDF     RRKIIQYINL+KSD    SHG+ +TT  G Q  WL A  FE D  AGHGTH AGS AGA LNT +E ++C  ++ + CVGAC+ D    + DDL T        DLDRLCP F   DC   + E CLSDDV +TL ++GGMAQGAKLAIFD FY+  G     GNG+WE C EA CK+HSNSYGGD  C +G  DV YD +M +NPENLL+FAAGN+G  +  R  CT+ SPAIAKNVLA+G+++SG TRL+ T            G  IDTV+ FSSYGPT D RIKPE++APGD IYSAASD + + ++CRLWA+ GTSMS P+VAGA+AM+RQYF+++TF+A D+TARG C +   CE FSPSSAT+KAL+INSANLMG SS+PD  RGFGRIH+EAGMPL GEG L LFV+DS  T+I  +  Q+Y FDV++ A LDLR T+SW+DP ATTFS  QL+HDLDL VI+P+G  + MW++G+ D+ NVNERVIVDA DV  GTW++ + A+ LTTDVQSYSLVVNGAISP TG
Sbjct:   22 PSLELVENALSTTAKDDFNGHARAFEAAATWNGERLRHRQDETRAPHLCCAEYGHGRETFSRLKQFLSPEAVRVVSHSEDLGACFFASASHAQASAIIEDQEQFGLESFSPFPSALKLAPGLVDHSQRHDKDKETTTGLDRLSARHGARMRMSNVEGLSIELTPGTLASRSVEAEAFISDTLGDLMLASVDLHSTNFWSDQNMGGGEQLSFPGGAVRARDWSKAASVVHELSEAAGTSPGDICSWNRVSVHHAEGDVLLVSGLDHLLHSGRGAGGRSDGGESAELHIACFMGLVSFLASKPEVLRVAPRQSQALLNASARANIQSATVTDTPLTDAGLDGTGQVVQVIDSGVDESSCFFAHDESGDHVPHGHYFEE--WGINPPLTSS-SFSSPTSDDDDSLGWPRATDDHRTGSYYQIAEEFDGGDFTIFPDRRKIIQYINLIKSDDTEGSHGDSFTTSLGQQYPWLPADRFEYDYEAGHGTHVAGSVAGATLNTPAELISCKDTETMSCVGACVDDDEANDVDDLAT--PNVEIVDLDRLCPLFSDYDCDVLETEKCLSDDVAETLTNNGGMAQGAKLAIFDAFYDNFGLSPFVGNGLWEPCAEADCKIHSNSYGGDFECSLGPNDVLYDGWMVSNPENLLIFAAGNDG--ERRRSTCTMSSPAIAKNVLAVGASTSGDTRLSATP-----------GLGIDTVAVFSSYGPTSDSRIKPEVLAPGDAIYSAASDGSMDSHSCRLWAYAGTSMSTPIVAGASAMIRQYFMNETFFATDMTARGFCDEWAGCEAFSPSSATLKALMINSANLMGGSSEPDGSRGFGRIHLEAGMPLRGEGSLALFVSDSNYTSIPERTRQEYNFDVNATARLDLRVTLSWLDPPATTFSAVQLVHDLDLVVISPNGTRYTMWASGVTDSANVNERVIVDAGDVETGTWSVWIWADNLTTDVQSYSLVVNGAISPGTG 971          

BLAST of mRNA_F-serratus_M_contig646.17403.1 vs. uniprot
Match: D8LNG6_ECTSI (Subtilisin n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LNG6_ECTSI)

HSP 1 Score: 1017 bits (2629), Expect = 0.000e+0
Identity = 550/956 (57.53%), Postives = 660/956 (69.04%), Query Frame = 1

Query:    1 PSLSLVERALRTTAIHDFDGHDPAYQAAVHWDRQRLRDNFSHQRAPYLACAEYGEGREAMKSLKEFLSPEAVRPASNTVETGACFIVTASNEQATSISAHAEQFWLLSVGPVPSALKLAPGLLDHNNDGNSSE---------RLATTHGKRMRMANVEGLNIELAPGTLRMHAPEVISFAKSLLEDLTSKSLDLHVGNVWSDPVMTGADHHLTN-GGAWRAREWIRAADVVEELTEAVDTSPSDICSWESTAIRQASDNVLMVSGVDHLLTSGRGAGGQGEE-AVELHMACFMGLVSFLASRPEVLRVAPRHKTRLLNAAARANIQTATVTSTPLTDAGLDGTGEVIQVVDTGLDETSCFFIDDDGEEIEHGHYFEELAWVATYYLTPYYSFYRYTTDTAVHEVFNGGDFVYDTSRRKIIQYINLVKSDSEPSSHGEYYTTEQGAQITWLDAADFEQDDAAGHGTHTAGSAAGAMLNTASETVTCSSSDVLGCVGACIS--DSTTYEYDDLLTYVDQFYSADLDRLCPSFDCGGYDEEVCLSDDVEQTLADHGGMAQGAKLAIFDIFYEESGFGSSAGNGVWEACLEAGCKVHSNSYGGDLICEMGEVDVEYDDFMYNNPENLLVFAAGNEGDIDDGRDVCTIISPAIAKNVLAIGSTSSGKTRLTTTAADGEGSGDFNGGADIDTVSFFSSYGPTLDGRIKPELVAPGDMIYSAASDATEVYTCRLWAFQGTSMSCPVVAGAAAMVRQYFVDDTFYAADVTARGLCSDGFVCEGFSPSSATVKALLINSANLMGESSDPDYFRGFGRIHMEAGMPLGGEGDLVLFVADSANTNIAGQATQQYLFDVDSDAGLDLRATISWIDPAATTFSGKQLIHDLDLTVIAPSGATHRMWSTGIADANNVNERVIVDAVDVANGTWAISVRANELTTDVQSYSLVVNGAISPATGDAPVASES 2829
            P+L LVE AL TTA  DF GH  A++AA  W+ +RLR +    RAP+LACA YG+G EA   LK+FLSPEAVR  S++ + GACF  +AS+ QA +I    EQF L +  P PS LKLAPGL+DH    + +E         RL+  HG RMR  NVEGL++EL PGTL   + E  SF   +L DL S S+DLH  N WSDP M GA  HL++  GA RAR+W +AA VV EL+EA  T P DICSW    +  A D+ L+VSG+DHLL SGRGAGG  EE + ELH+ACFMGLVSFLA RPEVLRV+PR   R+LNAAAR  IQ+A+ T TPLTDAGLDGTGE+IQV+D+GLDETSC+FIDD GEE++HG+YF+EL   A Y  +       Y++D     +FNGGDF Y   RRK                                          AGHGTHTAGSAAGA LN  +ET+TC+ +D L CVGACI   D+T    DDL++   Q    DLDRLCP + C    +  CLSDDV +TL+++GGMAQGAKLAIFD+FY      SS GN +WE C EAGCK+HSNS G D  C +   D+  D FMY N ENLL+FAAGNEG I+  R  CTI +P I KN LAIG+++SG TR+ +T           G   IDTV+ FSS+G T D RIKPE+VAPGD +YSAASD T+ ++CRLWA++GTSMSCP+VAGAAAM+RQYFVD+TFY ADVTARG CSDGF+CEGFSPS+ATVKALLINSANLMG SS+PD FRGFGR+H+EAGMPL GEGDLVLFVAD+ +T+IAG   ++YLFDVD+DAGLDLRAT+SWIDP AT  S  QL+H+LDL V++P G T+RMW +   D  NVNERVIVDA DV  GTW + V AN L TD QSYSLVVNGAISP TG+    S S
Sbjct:   27 PALELVENALLTTAKDDFKGHVGAFEAAATWNGERLRHSQEETRAPHLACARYGDGHEASSRLKQFLSPEAVRVVSHSEDHGACFFASASHAQAAAIVEDQEQFGLENFSPFPSPLKLAPGLVDHTESHDEAEEEARSSGLDRLSARHGARMRKPNVEGLSVELTPGTLAARSSEAKSFINHMLGDLMSASVDLHSTNFWSDPGMDGAGEHLSSPAGAVRARDWRKAASVVHELSEAASTGPGDICSWNRIFVHHAGDDSLLVSGLDHLLFSGRGAGGSHEEESTELHVACFMGLVSFLAGRPEVLRVSPRPTKRVLNAAARGVIQSASATDTPLTDAGLDGTGEIIQVIDSGLDETSCYFIDDSGEEVDHGYYFDELGRAAVYSSS-------YSSDETA-TIFNGGDFSYYPERRK------------------------------------------AGHGTHTAGSAAGATLNDPAETITCNVTDTLSCVGACIDLLDAT----DDLVSISSQTDGTDLDRLCPMYGCDNSTDTTCLSDDVPETLSNNGGMAQGAKLAIFDVFYGGYDASSSIGNNLWEVCTEAGCKLHSNSLGADFECSIASWDILNDQFMYENAENLLIFAAGNEGGIE--RSTCTINTPGIGKNSLAIGASTSGDTRVISTV----------GATGIDTVAEFSSWGFTTDDRIKPEVVAPGDSVYSAASDGTDTHSCRLWAYEGTSMSCPIVAGAAAMIRQYFVDETFYTADVTARGFCSDGFICEGFSPSAATVKALLINSANLMGGSSEPDGFRGFGRVHLEAGMPLEGEGDLVLFVADALDTSIAGGTLEEYLFDVDADAGLDLRATLSWIDPPATATSAVQLVHNLDLVVVSPDGTTYRMWGSDTTDNRNVNERVIVDATDVVTGTWTVWVWANNLVTDSQSYSLVVNGAISPGTGEGASGSSS 916          

BLAST of mRNA_F-serratus_M_contig646.17403.1 vs. uniprot
Match: A0A2I6U1R2_SACJA (Subtilisin (Fragment) n=1 Tax=Saccharina japonica TaxID=88149 RepID=A0A2I6U1R2_SACJA)

HSP 1 Score: 925 bits (2391), Expect = 0.000e+0
Identity = 506/916 (55.24%), Postives = 617/916 (67.36%), Query Frame = 1

Query:    1 PSLSLVERALRTTAIHDFDGHDPAYQAAVHWDRQRLRDNFS-HQRAPYLACAEYGEGREAMKSLKEFLSPEAVRPASNTVETGACFIVTASNEQATSISAHAEQFWLLSVGPVPSALKLAPGLLDH----NNDGNSSERLATTHGKRMRMANVEGLNIELAPGTLRMHAPEVISFAKSLLEDLTSKSLDLHVGNVWSDPVMTGADHHLTNGGAWRAREWIRAADVVEELTEAVDTSPSDICSWESTAIRQASDNVLMVSGVDHLLTSGRGAGGQGEEAVELHMACFMGLVSFLASRPEVLRVAPRHKTRLLNAAARANIQTATVTSTPLTDAGLDGTGEVIQVVDTGLDETSCFFIDDDGEEIEHGHYFEELAWVATYYLTPYYSFYRYTTDTAVHEVFNGGDFVYDTSRRKIIQYINLVKSDSEPSSHGEYYTTEQGAQITWLDAADFEQDDAAGHGTHTAGSAAGAMLNTASET-VTCSSSDVLGCVGACISDSTTYEYDDLLTYVDQF--YSADLDRLCPSFDCGGYDEEVCLSDDVEQTLADHGGMAQGAKLAIFDIFYEESGFGSSAGNGVWEACLEAGCKVHSNSYGGDLICEMGEVDVEYDDFMYNNPENLLVFAAGNEGDIDDGRDVCTIISPAIAKNVLAIGSTSSGKTRLTTTAADGEGSGDFNGGADIDTVSFFSSYGPTLDGRIKPELVAPGDMIYSAASDATEVYTCRLWAFQGTSMSCPVVAGAAAMVRQYFVDDTFYAADVTARGLCSD-GFVCEGFSPSSATVKALLINSANLMGESSDPDYFRGFGRIHMEAGMPLGGEGDLVLFVADSANTNIAGQATQQYLFDVDSDAGLDLRATISWIDPAATTFSGKQLIHDLDLTVIAPSGATHRMWST-GIADANNVNERVIVDAVDVANGTW 2718
            PSLS+VE +L TTA   FDGH  A++ A  W  + LR     + RAP+L C EY  G + +  L+E LSP AVRP +++   GACF VTAS+ +A ++S  +  + L S+ P PSALK+APG+L+H    +    S  RL TTHG  MR  NVEGLN+EL+PGTL  H+PE   F   LL+DL S S+DLH GNVWSDPV    +H  T  GA R REW  AA +V +L  A  TSP DICSW+S ++  A +++L+VSG+DHLL    GA G  EEA EL +ACFMGLVSFL++R EVLRV+PRH  RLLNA AR  IQ+AT+T  PLTDAGLDGTG+VIQV+DTGLDETSCFFIDDDGEE+ HGHY+E L                                              ++ S S                          +DD  GHGTH AGSA GA + T +ET  TCS ++V GCVG CI    T   DDL T + ++  Y  D+DRLCP  DC    +  CLSDDV QTLADHGGMAQGAKLA FD+F + S      GNG+WE CLEAGCK+HS S G D  C + E DV YDDFMYNNPENL+V AAGN GD +DGR VCT+ SPAI KN LA+G+TSSG+TR+T T+ADG+     NG AD+DTV+ FSSYGPT DGRIKPE+VAPGD IYSA SD ++ ++CRL A  GTSM+CP+VAGA+AMVRQYF+D++FY AD  ARG   D G  CE FSPSSATVKALLINSANLMG SS+PD +RGFGRIH+E GMPL GEG L LFVADS+ T++    +Q ++FDVD++A LD RAT+SWIDP A++ SG QL+HDLDL V++PSGATH MW+T G  D+ NVNERVI+ A DV +GT+
Sbjct:   32 PSLSVVEGSLLTTAQDSFDGHAKAFETAAQWTGEILRSKEEDNARAPHLVCTEYNRGGKTLPRLQELLSPGAVRPVAHSSTHGACFFVTASHAEARAMS-DSPYYGLTSISPFPSALKVAPGVLEHGVSLDQASQSPGRLTTTHGHLMRKGNVEGLNMELSPGTLPAHSPEAGMFIAELLDDLMSDSIDLHGGNVWSDPVAANGEHLTTPAGAVRKREWSMAATLVHQLAVAGRTSPGDICSWDSISMHYAGNDLLLVSGLDHLLHKENGAIGSDEEATELRVACFMGLVSFLSNRLEVLRVSPRHADRLLNAVARTIIQSATITEAPLTDAGLDGTGQVIQVIDTGLDETSCFFIDDDGEEVPHGHYYEALG--------------------------------------------EIIPSSSSA------------------------RDDVEGHGTHCAGSALGATIRTPAETPTTCSGTEVPGCVGGCIDADRTSWGDDLFTLMPEYSPYDIDIDRLCPMIDCDDATDPRCLSDDVGQTLADHGGMAQGAKLAFFDVFADGSSGTHFVGNGLWEPCLEAGCKIHSMSIGADFECSVCEKDVLYDDFMYNNPENLVVVAAGNAGDTEDGRTVCTMYSPAIGKNALAVGATSSGETRITKTSADGDLIDGTNGYADVDTVAQFSSYGPTQDGRIKPEVVAPGDAIYSARSDGSDNHSCRLSAKSGTSMACPIVAGASAMVRQYFMDESFYVADQAARGWSDDSGRRCESFSPSSATVKALLINSANLMGGSSEPDGYRGFGRIHLEMGMPLDGEGSLALFVADSSYTSLPELCSQDFIFDVDANANLDFRATLSWIDPPASSASGVQLVHDLDLEVVSPSGATHIMWATSGEVDSRNVNERVIIAAEDVESGTY 878          

BLAST of mRNA_F-serratus_M_contig646.17403.1 vs. uniprot
Match: A0A2I6U1S5_SACJA (Subtilisin n=1 Tax=Saccharina japonica TaxID=88149 RepID=A0A2I6U1S5_SACJA)

HSP 1 Score: 912 bits (2356), Expect = 0.000e+0
Identity = 506/942 (53.72%), Postives = 627/942 (66.56%), Query Frame = 1

Query:    1 PSLSLVERALRTTAIHDFDGHDPAYQAAVHWDRQRLRDNFS-HQRAPYLACAEYGEGREAMKSLKEFLSPEAVRPASNTVETGACFIVTASNEQATSISAHAEQFWLLSVGPVPSALKLAPGLLDH----NNDGNSSERLATTHGKRMRMANVEGLNIELAPGTLRMHAPEVISFAKSLLEDLTSKSLDLHVGNVWSDPVMTGADHHLTNGGAWRAREWIRAADVVEELTEAVDTSPSDICSWESTAIRQASDNVLMVSGVDHLLTSGRGAGGQGEEAVELHMACFMGLVSFLASRPEVLRVAPRHKTRLLNAAARANIQTATVTSTPLTDAGLDGTGEVIQVVDTGLDETSCFFIDDDGEEIEHGHYFEELAWVATYYLTPYYSFYRYTTDTAVHEVFNGGDFVYDTSRRKIIQYINLVKSDSE--PSSHGEYYTTEQGAQITWLDAADFEQDDAAGHGTHTAGSAAGAMLNTASET-VTCSSSDVLGCVGACISDSTTYEYDDLLTYVDQ--FYSADLDRLCPSFDCGGYDEEVCLSDDVEQTLADHGGMAQGAKLAIFDIFYEESGFGSSAGNGVWEACLEAGCKVHSNSYGGDLICEMGEVDVEYDDFMYNNPENLLVFAAGNEGDIDDGRDVCTIISPAIAKNVLAIGSTSSGKTRLTTTAADGEGSGDFNGGADIDTVSFFSSYGPTLDGRIKPELVAPGDMIYSAASDATEVYTCRLWAFQGTSMSCPVVAGAAAMVRQYFVDDTFYAADVTARGLCSD-GFVCEGFSPSSATVKALLINSANLMGESSDPDYFRGFGRIHMEAGMPLGGEGDLVLFVADSANTNIAGQATQQYLFDVDSDAGLDLRATISWIDPAATTFSGKQLIHDLDLTVIAPSGATHRMWST-GIADANNVNERVIVDAVDVANGTWAISVRANELTTDVQSYSLVVNGAI 2790
            PSL +VE +L TTA   FDGH   ++ A  W    +R     + RAP+L C EY  G E +  L+E LSPEAVR  +++   GACF VTAS+ +A ++S +   + L S+ P PSALK+APG+L+H    +    S  RLATTHG  MR  NVEGLN+EL+PGTL  H+P+   F   LL+DL S S+DLH GNVWSDPV    +H  T  GA R REW  AA +V +L  A  TSP DICSW+S +I  A +++L+VSG+DHLL    GA G  EEA EL +ACFMGLVSFL+SR EV RV+PRH  RLLNA AR  IQ+AT+T  PLT AGLDGTG+VIQV                                                                      IQYINL+K+DS   P+S     TT  G ++ W+    F+ DD   HGTHTAGSA GA + T +ET  TCS ++V GCVG CI    T   DDLLT + +   Y AD+DR+CP   C   +E  CLSD VEQTLADHGGMAQGAKL+ FDI+     F    GNG+WE CLEAGCK+HS S+G DL C + + D+ YDDFMYNNPENL+V AAGN G+I+DGR VCT+ SPAI KN LA+G+TSSG+TR+  T+ADG+ +   NG +D+DTV+ FSSYGPT DGRIKPE+VAPGD +YSA SD ++V++CRL A  GTSM+CP+VAGA+AMVRQYF+D++FY AD  ARG C D G  CE FSPSSATVKALLINSANLMG SS+PD FRGFGRIH+E GMPL GEG L LFVADS+ T++   ++Q ++FDVD++A LD RAT+SWIDP A++ SG QL+HDLDL V++PSGATH MW+T G  D+ NVNERVI+ A +V +GT+ + V  N L TD Q+YSLVVNGA+
Sbjct:   32 PSLGVVEGSLLTTAHESFDGHAKGFETAAQWTGDIIRSKEEDNARAPHLVCTEYNRGGETLSRLQELLSPEAVRSVAHSSTHGACFFVTASHAEARAMSDN-PYYGLTSISPFPSALKVAPGVLEHGVSRDQASQSPGRLATTHGHLMRKGNVEGLNLELSPGTLPAHSPQAGMFIAELLDDLMSDSIDLHGGNVWSDPVAANGEHLTTPAGAVRKREWSMAATLVHQLAVAGRTSPGDICSWDSISIHHAGNDLLLVSGLDHLLHKESGAIGSDEEATELRVACFMGLVSFLSSRLEVSRVSPRHADRLLNAVARTIIQSATITKAPLTAAGLDGTGQVIQV----------------------------------------------------------------------IQYINLIKADSVSGPASS----TTSGGNRVWWVSPDGFQGDDVDDHGTHTAGSALGATIRTPAETPTTCSGTEVPGCVGGCIDADRTSWGDDLLTLMPENSSYVADIDRICPMLGCDEENEPWCLSDGVEQTLADHGGMAQGAKLSFFDIYDGNMLFEQFIGNGLWEPCLEAGCKIHSLSFGADLECAVRDTDLLYDDFMYNNPENLVVVAAGNAGEIEDGRTVCTMYSPAIGKNALAVGATSSGETRVGVTSADGDRADGTNGYSDVDTVAVFSSYGPTQDGRIKPEVVAPGDAVYSARSDGSDVHSCRLSAKSGTSMACPIVAGASAMVRQYFMDESFYLADQAARGWCDDSGRQCESFSPSSATVKALLINSANLMGGSSEPDGFRGFGRIHLEMGMPLDGEGSLALFVADSSYTSLPELSSQDFVFDVDANANLDFRATLSWIDPPASSTSGVQLVHDLDLEVVSPSGATHIMWATSGEVDSRNVNERVIIAAENVESGTYTVRVSTNHLLTDSQNYSLVVNGAM 898          

BLAST of mRNA_F-serratus_M_contig646.17403.1 vs. uniprot
Match: D8LQI1_ECTSI (Subtilisin n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LQI1_ECTSI)

HSP 1 Score: 837 bits (2162), Expect = 6.600e-289
Identity = 480/960 (50.00%), Postives = 629/960 (65.52%), Query Frame = 1

Query:    1 PSLSLVERALRTTAIHDFDGHDPAYQAAVHW---DRQRLRDNFSHQRAPYLACAEYGEGREAMKSLKEFLSPEAVRPASNTVETGACFIVTASNEQATSISAHAEQFWLLSVGPVPSALKLAPGLLDHNN-DGN-------------SSERLATTHGKRMRMANVEGLNIELAPGTLRMHA---PEVISFAKSLLEDLTSKSLDLHVGNVWSDPVMTGADHHLTNGGAWRAREWIRAADVVEELTEAVDTSPSDICSWESTAIRQASDNVLMVSGVDHLLTSGRGAGGQGEE-AVELHMACFMGLVSFLASRPEVLRVAPRHKTRLLNAAARANIQTATVTSTPLTDAGLDGTGEVIQVVDTGLDETSCFFIDDDGEEIEHGHYFEELAWVATYYLTPYYSFYRYTTDTAVHEVFNGGDFVYDTSRRKIIQYINLVKSDS-EPSSHGEYYTTEQGAQITWLDAADFEQDDAAGHGTHTAGSAAGAMLNTASETVTCSS-SDVLGCVGACISDSTTYEYDDLLTYVDQFYSADLDRLCPSFDCGGYDEEVCLSDDVEQTLADHGGMAQGAKLAIFDIFYEESGFGSSAGNGVWEACLEAGCKVHSNSYGGDLICEMGEVDVEYDDFMYNNPENLLVFAAGNEGDIDDGRDVCTIISPAIAKNVLAIGSTSSGKTRLTTTAADGEGSGDFNGGADIDTVSFFSSYGPTLDGRIKPELVAPGDMIYSAASDATEVYTCRLWAFQGTSMSCPVVAGAAAMVRQYFVDDTFYAADVTARGLCSDGFVCEGFSPSSATVKALLINSANLMGESSDPDYFRGFGRIHMEAGMPLGGEGDLVLFVADSANTNIAGQATQQYLFDVDSDAGLDLRATISWIDPAATTFSGKQLIHDLDLTVIAPSGATHRMWSTGIADANNVNERVIVDAVDVANGT----WAISVRANELTT-DVQSYSLVVNGAISP 2796
            PSL   E+ L TTA  DFD  + A+  A  W   DRQ        + +PYL+C E+GEGR A + L+ FL   AV+P S+T   GACF+VTAS  +A  +S+  E F   + G  P+ALK+APGLLDH + DG              S+ RL+TTHG  M++ +  GL +EL+PGTL  H+    +   F + LL+ L+S SLDLH    WSD VM G DH  +  GA R REW RAA +V E +E+   +PS +C WE   +  A+++VL+V+G+DHLL  GR   G  EE A EL MAC+MGLVSFLASR EVL+VA  ++   LNAAARA IQ+A+V STPLTDAGLDG+GEVIQ++D G++ET+CF    DG E+EHG+YF+ +A     + +   S   +  +      F GGDF YD+ RRK+IQYI+L+  D+ + SS G          + ++ A  F  DD  GHGTH A + AG+ +++ +E V+C+  S+V+GC G CI++S+T   +         Y  D++RLCP+F C G   + CL DD   TL++HGGMAQGAK+A  D+FY +  +G  AGNG+WE C +AGCK+HSNSYG DL C++  +DV YD++MY N E+LL+F+AGN G   D RD CTI SP IAKNVL +GSTSSG TRL+TT  DG G  D +  + ID VS FS YG T DGRIKPE+VAPGDMIYSA+ +  +  +C L A+ GTS S  V +GAAAMVRQYF D +FY+ADV +RG C  GF C+ FSP + TVKA+LINSAN+MG  ++PD  RGFGRIH+EAGMPLGG GD+ LFVADS++  +      ++ F+VD DAG++LRAT+SW DPAA++ SG QL++DLDL V AP G  + MW     D +NVNERVIV A  VA  +    W + VR  + T  D  S+SLVV GAISP
Sbjct:   25 PSLGFFEQTLLTTAGADFDS-EAAFAFASAWQAGDRQG-------RSSPYLSCVEHGEGRHAYRKLEGFLGARAVKPVSSTATHGACFMVTASPAEAAQLSSW-EDF--TTFGAFPAALKIAPGLLDHGSCDGPAAARDAATTTSDCSAGRLSTTHGASMQVDSAHGLMVELSPGTLPSHSGGDAKAHEFIQELLKSLSSPSLDLHAITCWSDLVMKGGDHLFSAAGALRGREWSRAATLVNERSESEQETPSQVCGWEQVVVHHAANDVLLVAGLDHLLYDGREVSGASEEEAAELRMACYMGLVSFLASRHEVLKVAGWNRKVNLNAAARATIQSASVDSTPLTDAGLDGSGEVIQIIDAGVNETTCFLEHGDGTEVEHGYYFDFVASSNPLFTSSSESRSMFKAE------FTGGDFSYDSDRRKVIQYIDLINPDTGDGSSSG----------MPFVSADSFVSDDDLGHGTHAATTVAGSTISSPAELVSCADGSEVIGCAGGCITESSTGSSE-------ADYGEDINRLCPAFGCSG---DQCLPDDTADTLSEHGGMAQGAKIAAIDVFYGDYSYGDLAGNGLWETCADAGCKIHSNSYGVDLRCQLTALDVLYDEYMYENQEHLLLFSAGNTGWTTD-RDTCTIDSPGIAKNVLTVGSTSSGVTRLSTTDLDGRGYADGDELSTIDVVSEFSGYGFTEDGRIKPEVVAPGDMIYSASGNGGDS-SCELIAYAGTSFSTAVASGAAAMVRQYFRDSSFYSADVESRGFCDQGFTCDSFSPMATTVKAMLINSANVMGGGNEPDTERGFGRIHLEAGMPLGGAGDMALFVADSSDHEVYSDEKLKFYFNVDPDAGMELRATLSWNDPAASSLSGDQLVNDLDLRVYAPDGTRYCMWDDNCWDTSNVNERVIVPAEVVAQFSATDEWEVRVRGRDFTVGDTVSFSLVVTGAISP 945          

BLAST of mRNA_F-serratus_M_contig646.17403.1 vs. uniprot
Match: A0A6H5K365_9PHAE (Subtilisin n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5K365_9PHAE)

HSP 1 Score: 766 bits (1978), Expect = 3.630e-265
Identity = 420/705 (59.57%), Postives = 510/705 (72.34%), Query Frame = 1

Query:  751 LMVSGVDHLLTSGRGAGGQGEE-AVELHMACFMGLVSFLASRPEVLRVAPRHKTRLLNAAARANIQTATVTSTPLTDAGLDGTGEVIQVVDTGLDETSCFFIDDDGEEIEHGHYFEELAWVATYYLTPYYSFYRYTTDTAVHEVFNGGDFVYDTSRRKIIQYINLVKSDSEPSSHGEYYTTEQGAQITWLDAAD-FEQDDAAGHG--------THTAGSAAGAMLNTASETVTCSSSDVLGCVGACIS--DSTTYEYDDLLTYVDQFYSADLDRLCPSFDCGGYDEEVCLSDDVEQTLADHGGMAQGAKLAIFDIFYEESGFGSSAGNGVWEACLEAGCKVHSNSYGGDLICEMGEVDVEYDDFMYNNPENLLVFAAGNEGDIDDGRDVCTIISPAIAKNVLAIGSTSSGKTRLTTTAADGEGSGDFNGGADIDTVSFFSSYGPTLDGRIKPELVAPGDMIYSAASDATEVYTCRLWAFQGTSMSCPVVAGAAAMVRQYFVDDTFYAADVTARGLCSDGFVCEGFSPSSATVKALLINSANLMGESSDPDYFRGFGRIHMEAGMPLGGEGDLVLFVADSANTNIAGQATQQYLFDVDSDAGLDLRATISWIDPAATTFSGKQLIHDLDLTVIAPSGATHRMWSTGIADANNVNERVIVDAVDVANGTWAISVRANELTTDVQSYSLVVNGAISPATGDAPVASES 2829
            +  +G+DHLL SGRGAGG  EE + ELH+ACFMGLVS LA RPEVLRV+PR   R+LNAAAR  +Q+A+ T TPLTDAGLDGTGE+IQV+D+GLDETSC+FIDD GEE++HG+YF+ELA  A Y  +  YS    + +TA   +FNGGDF Y   RRKIIQYI+LV  DS  SS   +YT+ QG+ I +    D F +DD A                SAAGA LN  +ET+TC+ +D L CVGACI   D T    DDL++   Q    DLDRLCP + C    +  CLSDDV +TL+++GGMAQGAKLAIFD+FY +    SS GN +WE C EAGCK+HSNS G D  C +   D+  D FMY N E+LL+FAAGNEG   + R  CTI +P I KN LAIG+++SG TR+ +T           G   I+TV+ FSS+G T D RIKPE+VAPGD +YSAASD T+ ++CRLWA++GTSMSCP+VAGAAAM+RQYF+D+TFY ADVTARG CSD F+CEGFSPS+ATVKALLINSANLMG SS+PD FRGFGR+H+EAGMPL GEG+LVLFVAD+ +T+IA     +YLFDVD+DAGLDLRAT+SWIDP +T  S  QL+H+LDL V++P G T+RMW +   D  NVNERVIVDA DV  GTW + V AN L TD QSYSLVVNGAISP TG+    S S
Sbjct:    1 MHATGLDHLLFSGRGAGGSHEEESTELHVACFMGLVSVLAGRPEVLRVSPRPTKRVLNAAARGIVQSASATDTPLTDAGLDGTGEIIQVIDSGLDETSCYFIDDSGEEVDHGYYFDELAQAAVY--SSLYS----SDETAT--IFNGGDFSYYPERRKIIQYIDLVNPDSPYSS---FYTS-QGSYIGYFLPPDQFGEDDEARVALRKKDPSQARVGRSAAGATLNNPAETITCNVTDTLSCVGACIDLLDVT----DDLVSISHQTGGTDLDRLCPMYGCDNSTDTTCLSDDVPETLSNNGGMAQGAKLAIFDVFYGDYDASSSIGNNLWEVCTEAGCKLHSNSLGADFECSIASWDILNDQFMYENAEHLLIFAAGNEG--GEERSTCTINTPGIGKNSLAIGASTSGDTRVISTV----------GATGINTVAEFSSWGFTTDDRIKPEVVAPGDSVYSAASDGTDTHSCRLWAYEGTSMSCPIVAGAAAMIRQYFMDETFYMADVTARGFCSDVFICEGFSPSAATVKALLINSANLMGGSSEPDGFRGFGRVHLEAGMPLEGEGNLVLFVADALDTSIAEGTLDEYLFDVDADAGLDLRATLSWIDPPSTATSAVQLVHNLDLVVVSPDGTTYRMWGSETTDNRNVNERVIVDATDVVTGTWTVWVWANSLITDSQSYSLVVNGAISPGTGEGASGSSS 677          

BLAST of mRNA_F-serratus_M_contig646.17403.1 vs. uniprot
Match: D8LNG4_ECTSI (Subtilisin n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LNG4_ECTSI)

HSP 1 Score: 760 bits (1962), Expect = 2.590e-262
Identity = 403/686 (58.75%), Postives = 495/686 (72.16%), Query Frame = 1

Query:  844 MGLVSFLASRPEVLRVAPRHKTRLLNAAARANIQTATVTSTPLTDAGLDGTGEVIQVVDTGLDETSCFFI-DDDGEEIEHGHYFEELA-------------------------WV-ATYYLTPYYSFYRYTTDTAVHEVFNGGDFVYDTSRRKIIQYINLVKSDSEPSSHGEYYTTEQGAQITWLDAADFEQDDAAGHGTHTAGSAAGAMLNTASETVTCSSSDVLGCVGACISDSTTYEYDDLLT-YVDQFYSADLDRLCPSF---DCGGYDEEVCLSDDVEQTLADHGGMAQGAKLAIFDIFYEESGFGSSAGNGVWEACLEAGCKVHSNSYGGDLICEMGEVDVEYDDFMYNNPENLLVFAAGNEGDIDDGRDVCTIISPAIAKNVLAIGSTSSGKTRLTTTAADGEGSGDFNGGADIDTVSFFSSYGPTLDGRIKPELVAPGDMIYSAASDAT-EVYTCRLWAFQGTSMSCPVVAGAAAMVRQYFVDDTFYAADVTARGLCSDGFVCEGFSPSSATVKALLINSANLMGESSDPDYFRGFGRIHMEAGMPLGGEGDLVLFVADSANTNIAGQATQQYLFDVDSDAGLDLRATISWIDPAATTFSGKQLIHDLDLTVIAPSGATHRMWSTGIADANNVNERVIVDAVDVANGTWAISVRANELTTDVQSYSLVVNGAISPATG 2805
            MGLVSFLAS+PEVLRVAPR    LLNAAARANIQ+ATVT TPLTDAGLDGTG+V+QV+D+G+DE+SCFF  D+ G+ + HGHYFEE                           W  A ++   YY          + E F+GGDF     RRKIIQYINL+KSD    SHG+ +TT  G Q  WL A  FE DD AGHGTH AGS AGA LNT +E ++C+ ++ + CVGAC+ D    + DDL T YV+    ADLDRLCP F   DC G + + CLSDDV +TL ++GGMAQGAKLAIFD FY+  G     G G+WE C +A CK+HSNSYGGD  C +G  DV YD++M +NPENLL+FAAGN+G++   R  CTI SPAIAKNVLA+G+++SG TRL+ T   G           IDTV+ FSSYGPT D RIKPE++APGD IYSAASD + + ++CRLWA+ GTSMS P+VAGA+AM+RQYF+++TF+A D+TARG C +   CE FSPSSAT+KAL+INSANLMGESS+P+  RGFGRIH+EAGMPLGGEG L LFV+DS  T+I     Q+Y FDV++ AGLDLR T+SW+DP ATTFS  QL+HDLDL VI+P+G  + MW++G+ D++NVNERVIVDA DV  GTW++ + A+ LTTDVQSYSLVVNGAISP TG
Sbjct:    1 MGLVSFLASKPEVLRVAPRQSQALLNAAARANIQSATVTDTPLTDAGLDGTGQVVQVIDSGVDESSCFFAHDESGDHVPHGHYFEEWGINPSLTSSSFSSPXXXXXXXXXXXPWATADHHTGSYYQ---------IAEEFDGGDFTIYPDRRKIIQYINLIKSDDTEGSHGDSFTTSLGQQYPWLSADHFEYDDEAGHGTHVAGSVAGATLNTPAELISCNDTETMSCVGACVDDDEANDVDDLATPYVE---IADLDRLCPLFSDYDCDGLETDKCLSDDVAETLTNNGGMAQGAKLAIFDAFYDTFGLSPFVGTGLWEPCADADCKIHSNSYGGDFECSLGSNDVLYDEWMVSNPENLLIFAAGNDGELR--RSTCTIGSPAIAKNVLAVGASTSGDTRLSATPGSG-----------IDTVAVFSSYGPTSDSRIKPEVLAPGDAIYSAASDGSVDTHSCRLWAYVGTSMSTPIVAGASAMIRQYFMNETFFATDMTARGFCDEWAGCEAFSPSSATLKALMINSANLMGESSEPNGSRGFGRIHLEAGMPLGGEGSLALFVSDSNYTSIPELTRQEYNFDVNATAGLDLRVTLSWLDPPATTFSAVQLVHDLDLVVISPNGTRYTMWASGVTDSSNVNERVIVDADDVETGTWSVWIWADNLTTDVQSYSLVVNGAISPGTG 661          

BLAST of mRNA_F-serratus_M_contig646.17403.1 vs. uniprot
Match: A0A6H5JKA8_9PHAE (Subtilisin n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JKA8_9PHAE)

HSP 1 Score: 765 bits (1975), Expect = 7.130e-259
Identity = 489/1066 (45.87%), Postives = 623/1066 (58.44%), Query Frame = 1

Query:    1 PSLSLVERALRTTAIHDFDGHDPAYQAAVHWDRQRLRDNFSHQRAPYLACAEYGEGREAM-KSLKEFLSPEAV--RPASNTVETGACFIVTASNEQATSISAHAEQFWLLSVGPVPSALKLAPGLLDHNND----GNSSERLATTHGKRMRM----------------------------------------------ANVEGLNIELAPGTLRMHAPEVIS----FAKS------LLEDLTSKSLDLHVGNVWS---------DPVMTGADHHLTNG-GAWRAREWIRAADVVEEL--TEAVDTSPSDICSWESTAIRQASDNVLMVSGVDHLLTSGRGAGG-QGEEAVELHMACFMGLVSFLASRPEVLRVAPRHKTRLLNAAARANIQTATVTSTPLTDAGLDGTGEVIQVVDTGLDETSCFFIDDDGEE-IEHGHYFEELAWVATYYLTPYYSFYRYTTDTAVHEVFNGGDFVYDTSRRKIIQYINLVKSDSEPS--------------------------SHGEYYTTEQGAQITWLDAADFEQDDAAGHGTHTAGSAAGAMLNTASETVT------CSSSDVLGCVGACISDSTTYEYDDLLTYVDQFY------SADLDRLCPSFDCGGYDEEVCLSDDVEQTLADHGGMAQGAKLAIFDIFYEESGFG-SSAGNGVWEACLEAGCKVHSNSYGGDLICEMGEVDVEYDDFMYNNPENLLVFAAGNEGDIDDGRDVCTIISPAIAKNVLAIGSTSSGKTRLTTTAADGEGSGDFNGGADIDTVSFFSSYGPTLDGRIKPELVAPGDMIYSAASDATEVYTCRLWAFQGTSMSCPVVAGAAAMVRQYFVDDTFYAADVTARGLCSDGFVCEGFSPSSATVKALLINSANLMGESSDPDYFRGFGRIHMEAGMPLGGEGDLVLFVADSANTNIAGQATQQYLFDVDSDAGLDLRATISWIDPAATTFSGKQLIHDLDLTVIAPSGATHRMWSTGIADANNVNERVIVDAVDV---------------ANGTWAISVRANELT-TDVQSYSLVVNGAISPAT 2802
            P LS++  AL TTA   F  H   ++AAV W   RL D    + APYLACA     R     +L   L   A+  R   ++ E G C + TA+  QA SI    + F   S    PSALKLAPGLLDH +D    G    RL  THG+R+R                                               + V GL +ELAPG L        S    +A S        E+L S+SLD+H  + WS         +    GA      G G   AREW RAADV+  L  TE   T   D+CSW+   +    D++L + G+DHLL  GRGAGG +G EA EL +ACFM L+SFLASRPEVLRV+P    +LLNAAA  N+Q+A++T +P+TDAGLDGTGEVIQ+VDTGLD  SCFF+D+DG E + HG+YFEE+   A+ + + + S   +  +  +  VF GGDF +D SRRK +       S   P+                          ++GE + T  G Q  W+ AA+F +DDA GHGTHTAGSAAGA++++ S + T      C     L CVG CI     +  DDL+T  D  Y      + DLDRLCP  DC    EE CL DD  +TL  HGG+A+GA+LAIFDI  + +G G   AGNG+WE  +EAG K+ SNS+G    C+   +DV +DDFMY N + LL+FAAGN+GD   G   CT+ +PA+AKNVLA+G+++SG  RL+ T  DG+ +   +   DID +++FSS+GPTLD RIKPE+VAPGDMIYSAA      YTCRLW + GTSMS P+VAGAAA+VRQYF ++TFYA DV  RG C D F CE F+PS+ATVKA+LINSANLMG S++PD  RGFGR+H+EAGMPL GEGD  LFVADSA T+ A     +Y F VD+ AG+DLRAT+SWIDP ATTFS  QL+HDLDL+V +P+G  + MW  G  D+ N NERVIV A ++               ++GTW + V +  L   + QSYSLVV GAI P T
Sbjct:   30 PPLSVIRGALLTTAGVTFR-HQGEFKAAVEWHEARLDDA---RPAPYLACASPDSDRGGSGATLSHVLGAGAIGGRVVVSSAEHGVCVVATATPGQAESIFQGQQVF--SSWAAFPSALKLAPGLLDHPHDDRDGGGGDGRLRMTHGERLRRPKDRKGXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXDNIGGGPSGVTGLTVELAPGVLAATNRRGSSGGGWYASSGELTDEWREELMSQSLDVHDKSFWSSGWSAKLPDEAAAEGAGDSSGGGPGEVLAREWTRAADVLRGLGGTEGGPTI-GDVCSWDGLVVVHTDDDLLTMKGMDHLLPEGRGAGGNEGPEADELKVACFMALLSFLASRPEVLRVSPARAIKLLNAAAYHNVQSASLTESPMTDAGLDGTGEVIQIVDTGLDVNSCFFVDEDGGEGVAHGYYFEEIGVEASSFGSWFTS---WDFNVQIARVFTGGDFNFDLSRRKGLVVDAAHPSCDSPNISRRFALGDVRAWSGENPACTANNSTNYGESWATSAGEQYPWISAAEFAEDDAGGHGTHTAGSAAGAVVSSPSYSQTGDFTEVCEEGRELSCVGGCIDVDDPFGEDDLITTWDYQYFWAGYEAIDLDRLCPRLDCDDLPEEYCLGDDEAETLQQHGGVARGAQLAIFDIINDYTGVGIDMAGNGMWEPAMEAGSKLSSNSWGIYAYCDYLSLDVLFDDFMYRNQDQLLLFAAGNDGDDWSG---CTMGTPALAKNVLAVGASTSGPGRLSFTDEDGDPTDGDSRVQDIDAMAYFSSFGPTLDNRIKPEVVAPGDMIYSAAGSGDGNYTCRLWQYAGTSMSTPIVAGAAALVRQYFKNETFYAHDVEERGFC-DSFGCEPFAPSAATVKAMLINSANLMGGSAEPDGLRGFGRVHLEAGMPLAGEGDNALFVADSAVTSTAELTLTEYSFAVDAAAGIDLRATLSWIDPPATTFSSVQLVHDLDLSVYSPTGLRYSMWRDGTVDSLNNNERVIVPASNILDQTSSDATTAEDGESDGTWTVRVWSKRLAGNETQSYSLVVTGAIGPPT 1081          

BLAST of mRNA_F-serratus_M_contig646.17403.1 vs. uniprot
Match: D8LSW0_ECTSI (Subtilisin n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LSW0_ECTSI)

HSP 1 Score: 682 bits (1761), Expect = 2.030e-228
Identity = 437/1040 (42.02%), Postives = 582/1040 (55.96%), Query Frame = 1

Query:    7 LSLVERALRTTAIHDFDGHDPAYQAAVHWDRQRLR-DNFSHQRAPYLACAEYGEGREAMKSLKEFLSPEAVRPASNTVETGACFIVTASNEQATSISAHAEQFWLLSVGPVPSALKLAPGLLDHNND-------------GNSSERLATTHGKRMRMANVEGLNIELAPGTLRMHAPEVISFAKSLLED----LTSKSLDLHVGNVWSDPVMTGADHHLTNGGAWRAREWIRAADVVEELTEAVDTSPSDICSWESTAIRQASDNVLMVSGVDHLLTSGRGAGGQGEEAVELHMACFMGLVSFLASRPEVLRVAPRHKTRLLNAAARANIQTATVTSTPLTDAGLDGTGEVIQVVDTGLDETSCFFIDDDGEEIEHGHYFEELAWVATYYLTPYYSFYRYTTDTAVHEVFNGGDFVYDTSRRKIIQYINLVKS---------------------------DSEPSSH----GEYY---------------------------TTEQGAQITWLDAADFEQDDAAGHGTHTAGSAAGAM-LNTASETVTCSSSDVLGCVGACISDSTTYEYDDLLTYVDQFYSADLDRLCPSFDCGGYDE---EVCLSDDVEQTLADHGGMAQGAKLAIFDIFYEESG-FGSSAGNGVWEACLEAGCKVHSNSYGGDLICEMGEVDVEYDDFMYNNPENLLVFAAGNEGDIDD--GRDVCTIISPAIAKNVLAIGSTSSGKTRLTTTAADGE------GSGDFNGG--------------------ADIDTVSFFSSYGPTLDGRIKPELVAPGDMIYSAASDATEVYTCRLWAFQGTSMSCPVVAGAAAMVRQYFVDDTFYAADVTARGLC---SDGFVCEGFSPSSATVKALLINSANLMGESSDPDYFRGFGRIHMEAGMPLGGEGDLVLFVADSANTNIAGQATQQYLFDVDSDAGLDLRATISWIDPAATTFSGKQLIHDLDLTVIAPSGATHRMWSTGIADANNVNERVIVDAVDVANGTWAISVRANELTTDVQSYSLVVNGAI 2790
            L  ++    ++A  DF     AY AA  W   RL  D      APY+ACA+Y +GR A+ SL+   +  AV   SNT   G CFIVTA    AT++    E F LLS  P+  +LKLA  LLDH  D              + S  L +T+G+ M + NV GL++ L+PG L     E  SFA++ L D    LTS+S+++   + WSD     +++ +T     R REW RAA VV+ L      +  +IC +    +R  +D++++V G++HLL   R          E   AC+MGL+S LA++PEVLRV+P    RL NA A A +Q+AT T TPL DAGLDGTGEVIQVVD+GLDETSCFF DDDG ++EHG  F+ +               R   D  V  V     F +D SRRKI+QYI L K+                           DSE  S+    GEYY                           T+   +  +      F +D   GHGT TAGSAAGA+   +  E   C   ++ GC G CIS S      D  T+       DLD  CP + C G  +     CLSDD  + L  HGG+A GA++++FD+ Y  +  + S AGN +W + +E G K+H+NS+GG  +C++ E +  YD FMY NPE+LL+FAAGN+G  +D   R+ CT+ SPA+ KN LA+G+TSSG    T T  DG       G  D++                      A +DTV+FFSS GPTLDGRIKP++VAPGD + SA+SD T+ ++C+L A  GTS SCP+VAGAAA+VRQYF D +F+A DV +RG C   S    C  F+PS+ATVKA++INSA+LMG SS+P+  RGFGR+H+EAGMP+ G G+  L V ++A  +  G+ T+  L  VD DAG++LRAT++W+DP  T  S KQL HDLDL VIAPSGA + MW +G+ D  NV ERVIV    + +G W++ V A  L TD Q YSLVV GAI
Sbjct:   14 LGKIQAITASSAFDDFARSQSAYSAASSWYNDRLSYDAAEGSTAPYVACADYKQGRHALTSLETAFTKSAVHRVSNTEADGTCFIVTALPSSATAVIRAPEDFDLLSAAPLLPSLKLATSLLDHGPDTSPRLGAADDFDLSDHSAPLRSTYGEGMTLDNVHGLSVRLSPGVLTAGGRE--SFAEAFLRDWHADLTSESVNMRRASFWSDSDADRSEYDVT-----RVREWSRAAAVVDGLASKSARTVGEICGFGKLKLRHVADDLVLVEGMNHLLPGKRFPS-------ETRTACYMGLLSQLAAKPEVLRVSPLQSARLHNAVANALVQSATTTETPLLDAGLDGTGEVIQVVDSGLDETSCFFADDDGLQVEHGFLFDGV---------------RVEDDGNVSTVLGNYSFPFDMSRRKIVQYIQLFKTPDQGSTSSGQDSFTYVSDYLGDPYDYDDSEYISYDDYSGEYYSDTWSEDSFCAGVLDDLSYHFSYLFDDTSYSSSSTSPSFTGGFHKDTVGGHGTWTAGSAAGAISAQSILEEEACYGDELPGCAGGCISASDVDAMLDDSTF-------DLDVFCPMYTCDGNTDIPSSECLSDDPVENLHQHGGVAPGAQISVFDVSYVGTEIYASFAGNDLWYSAMETGAKIHTNSWGGATLCQLTEDEYLYDTFMYENPEHLLIFAAGNDGGYEDIPNREACTVGSPALGKNSLAVGATSSGPFPGTRTGDDGNLVYEELGLTDYSPEGYPWICLTPFLGTPSASTEPAGVDTVAFFSSQGPTLDGRIKPDVVAPGDQVASASSDGTDGHSCKLVASMGTSASCPLVAGAAALVRQYFKDPSFFAKDVVSRGGCAEHSSSCQCSEFAPSAATVKAMIINSAHLMGGSSEPNGVRGFGRVHLEAGMPMDGVGETGLSVVEAAIAD--GEITE--LVTVDGDAGVELRATLAWLDPPTTALSAKQLQHDLDLHVIAPSGAKYTMWESGVDDTVNVVERVIVPVESMESGVWSVVVSAKALLTDEQLYSLVVTGAI 1013