mRNA_F-serratus_M_contig646.17401.1 (mRNA) Fucus serratus male

BLAST of mRNA_F-serratus_M_contig646.17401.1 vs. uniprot
Match: A0A6H5K229_9PHAE (Subtilisin n=2 Tax=Ectocarpus TaxID=2879 RepID=A0A6H5K229_9PHAE)

HSP 1 Score: 1072 bits (2773), Expect = 0.000e+0
Identity = 564/942 (59.87%), Postives = 686/942 (72.82%), Query Frame = 1

Query:    1 PSLSLLESVLRTTAIHDFDGHDPAYQAAVRWDRQRLRDKFSNQRAPYLACAGYGKGRGAMNGLKEFLSPEAVRTASNAVETGACFIVTASNEQAASISAHPEEFSLSSVGPFPSALKLAPGLLDHKND-------GNSSERLTTTHGERMRMANVEGLSVELAPGILGMHTPEVLPFAKSLLEDLASKSLDLHNGNVWSDPAMAGADHHLTEGGAWRARQWIRAADVVEELAEAVDTSPSDICSWESTAIHQASANVLVVSGVDHLLASGRGVGGQGKE-ALELHMACFMGLVSFLANRPEVLRVAPRHETRLLNAAARANIQTATVTSAPLTDAGLDGTGEVIQVVDSGLDETSCFFIDDDGEEVEHGHYFEELAVFRVGFLTASSYDVYSSYTYVHGVYEGGDFSYDTSRRKIIQYISLVKSESEPSSHGEYYTTESGIQIPWVDAASFEQDYAAGHGTHTAGSAAGATLNTPAETVTCSSPDVLGCVGACISDSAAYSYDDLVTLSSQFYFADLDRLCPSFDCGGYDEEVCLSGDVDETLAKHGGMAQGAKLAIFDMFYGEFGFGSLAGNGVWEACSEAGCKVHSNSYGADYLCEMSEGDVEYDDFMYNNPENLLIFSAGNDGDIDDGRDVCTMGSPAIAKNLLAIGSTSSGETRLTKTTPDGEASRDFNGGADIDTVSYFSSYGPTRDGRIKPELVAPGDMIYSAASDGTDRYSCRLWAYQGTSMSCPIVAGAAAMVRQYFVDDSFYATDVTTRGFCGYGFVCDGFSPSSATVKALLINSANLMGGSSDPDDFRGFGRIHMEAGMPLGGEGGLVLFVADSANTNIAEQTMQQYIFGVDSDAGLDLRATISWIDPAATAFSEKQLIHNLDLTLIAPSGTTHTMWSTGIADANNVNERVIIDAADVENGTWAIRVWANELTTDVQSYSLVVNGAISPATG 2802
            PSL L+E+ L TTA  DF+GH  A++AA  W+ +RLR      RAP+L CA YG GR   + LK+FLSP+AVR  S++ + GACF+ TAS+ QA++I    E+F L S  PFPSALKLAPGL+DH           +  +RL   HG  MRM+NVEGL+V+L P  L   +     F   LL DL S S+DLH+ N WSDP MAG +H  + GGA RAR W +AA VV EL+EA  TSP DICSW+  A+ QA  + L+V+G+DHLL SGRG GG  +E + ELH+ACFMGLVSF A RPEVLRV+PRH  R+LN AAR  IQ+A+ T  PLTDAGLDGTGEVIQVVD+GLDETSCFFIDD GEEV+HG++FEEL V   G          SS    + V+E GDF+Y   RRK+IQYI LV  +S+   +  + TT+ G+  P++    F QD +AGHGTHTAGSAAGATLN PAET+TCS  + LGCVG CI +S   + DDLV+ + Q    DLDRLCP + C G   + CLS +VDETL K+GGMAQGAKL+IFD+F+  FG   + GNGVWE C EAGCKVHS S G D LC   E ++ YDDFMY NPENLLIF+AGNDG+ +  R VCTM SPAI KN+LA+G+++SG+TR+  T           GGA ID V+ FSSYG T DGRIKPE+VAPGDM+YSAASDGTD +SCRLWAYQGTSMSCPIVAGAAAMVRQYFVD++FY  DVT RGFC  GF+C+GFSPS+ATVKALLINSANLMG SS PD +RGFGRIH+E GMPL GEG L LFVAD+ +T+IAE T+Q+Y+F VD+DAGL+LRAT+SWID  AT+ S  QL+++LDL +++PSGT + MW + + D  NVNERVI+DA DVE GTW + V++N L TD QSYSLVVNGAIS  TG
Sbjct:   32 PSLELVENGLATTAKDDFNGHARAFEAAATWNGERLRRSQDGTRAPHLCCAEYGHGRETFSRLKQFLSPDAVRVVSHSEDHGACFLATASHAQASAIMEDQEQFGLESFSPFPSALKLAPGLVDHSESREVEGEPSSGQDRLGARHGGTMRMSNVEGLNVKLTPDTLPARSAGAESFIIDLLGDLMSASVDLHSTNFWSDPVMAGGEHLSSHGGAVRARDWSKAASVVHELSEAAGTSPGDICSWDEVALIQAGDDSLLVTGLDHLLFSGRGAGGSHEEESTELHVACFMGLVSFFAGRPEVLRVSPRHTKRVLNEAARGIIQSASATDTPLTDAGLDGTGEVIQVVDTGLDETSCFFIDDGGEEVDHGYFFEELFVGSTG----------SSQLIYYPVFEAGDFTYTEERRKVIQYIELVNPDSD---YVAFSTTQGGV-YPFLPPDEFGQDDSAGHGTHTAGSAAGATLNDPAETLTCSGDETLGCVGGCIDESE--TTDDLVSYAIQIALPDLDRLCPMYGCDG-STDPCLSDEVDETLTKNGGMAQGAKLSIFDVFFEVFGLIEVLGNGVWEPCLEAGCKVHSLSLGGDTLCTPGESEILYDDFMYENPENLLIFAAGNDGETE--RSVCTMASPAIGKNVLAVGASTSGDTRVIST----------EGGAGIDMVADFSSYGFTTDGRIKPEVVAPGDMVYSAASDGTDSHSCRLWAYQGTSMSCPIVAGAAAMVRQYFVDETFYTADVTARGFCSDGFICEGFSPSAATVKALLINSANLMGESSQPDAYRGFGRIHLEQGMPLEGEGDLALFVADALDTSIAEDTVQEYLFDVDADAGLELRATLSWIDYPATSTSAMQLVNDLDLVVVSPSGTVYRMWGSDVTDNRNVNERVIVDADDVETGTWTVLVYSNSLVTDSQSYSLVVNGAISAGTG 944          

BLAST of mRNA_F-serratus_M_contig646.17401.1 vs. uniprot
Match: A0A6H5JQT4_9PHAE (Subtilisin n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JQT4_9PHAE)

HSP 1 Score: 1063 bits (2748), Expect = 0.000e+0
Identity = 557/967 (57.60%), Postives = 686/967 (70.94%), Query Frame = 1

Query:    1 PSLSLLESVLRTTAIHDFDGHDPAYQAAVRWDRQRLRDKFSNQRAPYLACAGYGKGRGAMNGLKEFLSPEAVRTASNAVETGACFIVTASNEQAASISAHPEEFSLSSVGPFPSALKLAPGLLDH-------KNDGNSSERLTTTHGERMRMANVEGLSVELAPGILGMHTPEVLPFAKSLLEDLASKSLDLHNGNVWSDPAMAGADHHLTEGGAWRARQWIRAADVVEELAEAVDTSPSDICSWESTAIHQASANVLVVSGVDHLLASGRGVGGQ--GKEALELHMACFMGLVSFLANRPEVLRVAPRHETRLLNAAARANIQTATVTSAPLTDAGLDGTGEVIQVVDSGLDETSCFFI-DDDGEEVEHGHYFEELAVFRVGFLTASSYDVYSS-------------------YTYVHGVYEGGDFSYDTSRRKIIQYISLVKSESEPSSHGEYYTTESGIQIPWVDAASFEQDYAAGHGTHTAGSAAGATLNTPAETVTCSSPDVLGCVGACISDSAAYSYDDLVTLSSQFYFADLDRLCPSF---DCGGYDEEVCLSGDVDETLAKHGGMAQGAKLAIFDMFYGEFGFGSLAGNGVWEACSEAGCKVHSNSYGADYLCEMSEGDVEYDDFMYNNPENLLIFSAGNDGDIDDGRDVCTMGSPAIAKNLLAIGSTSSGETRLTKTTPDGEASRDFNGGADIDTVSYFSSYGPTRDGRIKPELVAPGDMIYSAASDGT-DRYSCRLWAYQGTSMSCPIVAGAAAMVRQYFVDDSFYATDVTTRGFCGYGFVCDGFSPSSATVKALLINSANLMGGSSDPDDFRGFGRIHMEAGMPLGGEGGLVLFVADSANTNIAEQTMQQYIFGVDSDAGLDLRATISWIDPAATAFSEKQLIHNLDLTLIAPSGTTHTMWSTGIADANNVNERVIIDAADVENGTWAIRVWANELTTDVQSYSLVVNGAISPATG 2802
            PSL L+E+ L TTA  DF+GH  A++AA  W+ +RLR +    RAP+L CA YG GR   + LK+FLSPEAVR  S++ + GACF  +AS+ QA++I    E+F L S  PFPSALKLAPGL+DH       K      +RL+  HG RMRM+NVEGLS+EL PG L   + E   F    L DL   S+DLH+ N WSD  M G +     GGA RAR W +AA VV EL+EA  TSP DICSW   ++H A  +VL+VSG+DHLL SGRG GG+  G E+ ELH+ACFMGLVSFLA++PEVLRVAPR    LLNA+ARANIQ+ATVT  PLTDAGLDGTG+V+QV+DSG+DE+SCFF  D+ G+ V HGHYFEE  +     LT+SS+   +S                   Y  +   ++GGDF+    RRKIIQYI+L+KS+    SHG+ +TT  G Q PW+ A  FE DY AGHGTH AGS AGATLNTPAE ++C   + + CVGAC+ D  A   DDL T + +    DLDRLCP F   DC   + E CLS DV ETL  +GGMAQGAKLAIFD FY  FG     GNG+WE C+EA CK+HSNSYG D+ C +   DV YD +M +NPENLLIF+AGNDG  +  R  CTM SPAIAKN+LA+G+++SG+TRL+ T            G  IDTV+ FSSYGPT D RIKPE++APGD IYSAASDG+ D +SCRLWAY GTSMS PIVAGA+AM+RQYF++++F+ATD+T RGFC     C+ FSPSSAT+KAL+INSANLMGGSS+PD  RGFGRIH+EAGMPL GEG L LFV+DS  T+I E+T Q+Y F V++ A LDLR T+SW+DP AT FS  QL+H+LDL +I+P+GT +TMW++G+ D+ NVNERVI+DA DVE GTW++ +WA+ LTTDVQSYSLVVNGAISP TG
Sbjct:   22 PSLELVENALSTTAKDDFNGHARAFEAAATWNGERLRHRQDETRAPHLCCAEYGHGRETFSRLKQFLSPEAVRVVSHSEDLGACFFASASHAQASAIIEDQEQFGLESFSPFPSALKLAPGLVDHSQRHDKDKETTTGLDRLSARHGARMRMSNVEGLSIELTPGTLASRSVEAEAFISDTLGDLMLASVDLHSTNFWSDQNMGGGEQLSFPGGAVRARDWSKAASVVHELSEAAGTSPGDICSWNRVSVHHAEGDVLLVSGLDHLLHSGRGAGGRSDGGESAELHIACFMGLVSFLASKPEVLRVAPRQSQALLNASARANIQSATVTDTPLTDAGLDGTGQVVQVIDSGVDESSCFFAHDESGDHVPHGHYFEEWGINPP--LTSSSFSSPTSDDDDSLGWPRATDDHRTGSYYQIAEEFDGGDFTIFPDRRKIIQYINLIKSDDTEGSHGDSFTTSLGQQYPWLPADRFEYDYEAGHGTHVAGSVAGATLNTPAELISCKDTETMSCVGACVDDDEANDVDDLATPNVEI--VDLDRLCPLFSDYDCDVLETEKCLSDDVAETLTNNGGMAQGAKLAIFDAFYDNFGLSPFVGNGLWEPCAEADCKIHSNSYGGDFECSLGPNDVLYDGWMVSNPENLLIFAAGNDG--ERRRSTCTMSSPAIAKNVLAVGASTSGDTRLSATP-----------GLGIDTVAVFSSYGPTSDSRIKPEVLAPGDAIYSAASDGSMDSHSCRLWAYAGTSMSTPIVAGASAMIRQYFMNETFFATDMTARGFCDEWAGCEAFSPSSATLKALMINSANLMGGSSEPDGSRGFGRIHLEAGMPLRGEGSLALFVSDSNYTSIPERTRQEYNFDVNATARLDLRVTLSWLDPPATTFSAVQLVHDLDLVVISPNGTRYTMWASGVTDSANVNERVIVDAGDVETGTWSVWIWADNLTTDVQSYSLVVNGAISPGTG 971          

BLAST of mRNA_F-serratus_M_contig646.17401.1 vs. uniprot
Match: D8LNG6_ECTSI (Subtilisin n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LNG6_ECTSI)

HSP 1 Score: 1025 bits (2649), Expect = 0.000e+0
Identity = 548/945 (57.99%), Postives = 657/945 (69.52%), Query Frame = 1

Query:    1 PSLSLLESVLRTTAIHDFDGHDPAYQAAVRWDRQRLRDKFSNQRAPYLACAGYGKGRGAMNGLKEFLSPEAVRTASNAVETGACFIVTASNEQAASISAHPEEFSLSSVGPFPSALKLAPGLLDHKNDGNSSE---------RLTTTHGERMRMANVEGLSVELAPGILGMHTPEVLPFAKSLLEDLASKSLDLHNGNVWSDPAMAGADHHLTE-GGAWRARQWIRAADVVEELAEAVDTSPSDICSWESTAIHQASANVLVVSGVDHLLASGRGVGGQGKE-ALELHMACFMGLVSFLANRPEVLRVAPRHETRLLNAAARANIQTATVTSAPLTDAGLDGTGEVIQVVDSGLDETSCFFIDDDGEEVEHGHYFEELAVFRVGFLTASSYDVYSSYTYVHGVYEGGDFSYDTSRRKIIQYISLVKSESEPSSHGEYYTTESGIQIPWVDAASFEQDYAAGHGTHTAGSAAGATLNTPAETVTCSSPDVLGCVGACISDSAAYSYDDLVTLSSQFYFADLDRLCPSFDCGGYDEEVCLSGDVDETLAKHGGMAQGAKLAIFDMFYGEFGFGSLAGNGVWEACSEAGCKVHSNSYGADYLCEMSEGDVEYDDFMYNNPENLLIFSAGNDGDIDDGRDVCTMGSPAIAKNLLAIGSTSSGETRLTKTTPDGEASRDFNGGADIDTVSYFSSYGPTRDGRIKPELVAPGDMIYSAASDGTDRYSCRLWAYQGTSMSCPIVAGAAAMVRQYFVDDSFYATDVTTRGFCGYGFVCDGFSPSSATVKALLINSANLMGGSSDPDDFRGFGRIHMEAGMPLGGEGGLVLFVADSANTNIAEQTMQQYIFGVDSDAGLDLRATISWIDPAATAFSEKQLIHNLDLTLIAPSGTTHTMWSTGIADANNVNERVIIDAADVENGTWAIRVWANELTTDVQSYSLVVNGAISPATG 2802
            P+L L+E+ L TTA  DF GH  A++AA  W+ +RLR      RAP+LACA YG G  A + LK+FLSPEAVR  S++ + GACF  +AS+ QAA+I    E+F L +  PFPS LKLAPGL+DH    + +E         RL+  HG RMR  NVEGLSVEL PG L   + E   F   +L DL S S+DLH+ N WSDP M GA  HL+   GA RAR W +AA VV EL+EA  T P DICSW    +H A  + L+VSG+DHLL SGRG GG  +E + ELH+ACFMGLVSFLA RPEVLRV+PR   R+LNAAAR  IQ+A+ T  PLTDAGLDGTGE+IQV+DSGLDETSC+FIDD GEEV+HG+YF+EL    V    +SSY    + T    ++ GGDFSY   RRK                                          AGHGTHTAGSAAGATLN PAET+TC+  D L CVGACI    A   DDLV++SSQ    DLDRLCP + C    +  CLS DV ETL+ +GGMAQGAKLAIFD+FYG +   S  GN +WE C+EAGCK+HSNS GAD+ C ++  D+  D FMY N ENLLIF+AGN+G I+  R  CT+ +P I KN LAIG+++SG+TR+  T           G   IDTV+ FSS+G T D RIKPE+VAPGD +YSAASDGTD +SCRLWAY+GTSMSCPIVAGAAAM+RQYFVD++FY  DVT RGFC  GF+C+GFSPS+ATVKALLINSANLMGGSS+PD FRGFGR+H+EAGMPL GEG LVLFVAD+ +T+IA  T+++Y+F VD+DAGLDLRAT+SWIDP ATA S  QL+HNLDL +++P GTT+ MW +   D  NVNERVI+DA DV  GTW + VWAN L TD QSYSLVVNGAISP TG
Sbjct:   27 PALELVENALLTTAKDDFKGHVGAFEAAATWNGERLRHSQEETRAPHLACARYGDGHEASSRLKQFLSPEAVRVVSHSEDHGACFFASASHAQAAAIVEDQEQFGLENFSPFPSPLKLAPGLVDHTESHDEAEEEARSSGLDRLSARHGARMRKPNVEGLSVELTPGTLAARSSEAKSFINHMLGDLMSASVDLHSTNFWSDPGMDGAGEHLSSPAGAVRARDWRKAASVVHELSEAASTGPGDICSWNRIFVHHAGDDSLLVSGLDHLLFSGRGAGGSHEEESTELHVACFMGLVSFLAGRPEVLRVSPRPTKRVLNAAARGVIQSASATDTPLTDAGLDGTGEIIQVIDSGLDETSCYFIDDSGEEVDHGYYFDELGRAAV---YSSSYSSDETAT----IFNGGDFSYYPERRK------------------------------------------AGHGTHTAGSAAGATLNDPAETITCNVTDTLSCVGACIDLLDAT--DDLVSISSQTDGTDLDRLCPMYGCDNSTDTTCLSDDVPETLSNNGGMAQGAKLAIFDVFYGGYDASSSIGNNLWEVCTEAGCKLHSNSLGADFECSIASWDILNDQFMYENAENLLIFAAGNEGGIE--RSTCTINTPGIGKNSLAIGASTSGDTRVISTV----------GATGIDTVAEFSSWGFTTDDRIKPEVVAPGDSVYSAASDGTDTHSCRLWAYEGTSMSCPIVAGAAAMIRQYFVDETFYTADVTARGFCSDGFICEGFSPSAATVKALLINSANLMGGSSEPDGFRGFGRVHLEAGMPLEGEGDLVLFVADALDTSIAGGTLEEYLFDVDADAGLDLRATLSWIDPPATATSAVQLVHNLDLVVVSPDGTTYRMWGSDTTDNRNVNERVIVDATDVVTGTWTVWVWANNLVTDSQSYSLVVNGAISPGTG 908          

BLAST of mRNA_F-serratus_M_contig646.17401.1 vs. uniprot
Match: A0A2I6U1R2_SACJA (Subtilisin (Fragment) n=1 Tax=Saccharina japonica TaxID=88149 RepID=A0A2I6U1R2_SACJA)

HSP 1 Score: 926 bits (2392), Expect = 0.000e+0
Identity = 507/915 (55.41%), Postives = 614/915 (67.10%), Query Frame = 1

Query:    1 PSLSLLESVLRTTAIHDFDGHDPAYQAAVRWDRQRLRDKFS-NQRAPYLACAGYGKGRGAMNGLKEFLSPEAVRTASNAVETGACFIVTASNEQAASISAHPEEFSLSSVGPFPSALKLAPGLLDH----KNDGNSSERLTTTHGERMRMANVEGLSVELAPGILGMHTPEVLPFAKSLLEDLASKSLDLHNGNVWSDPAMAGADHHLTEGGAWRARQWIRAADVVEELAEAVDTSPSDICSWESTAIHQASANVLVVSGVDHLLASGRGVGGQGKEALELHMACFMGLVSFLANRPEVLRVAPRHETRLLNAAARANIQTATVTSAPLTDAGLDGTGEVIQVVDSGLDETSCFFIDDDGEEVEHGHYFEELAVFRVGFLTASSYDVYSSYTYVHGVYEGGDFSYDTSRRKIIQYISLVKSESEPSSHGEYYTTESGIQIPWVDAASFEQDYAAGHGTHTAGSAAGATLNTPAET-VTCSSPDVLGCVGACISDSAAYSYDDLVTLSSQF--YFADLDRLCPSFDCGGYDEEVCLSGDVDETLAKHGGMAQGAKLAIFDMFYGEFGFGSLAGNGVWEACSEAGCKVHSNSYGADYLCEMSEGDVEYDDFMYNNPENLLIFSAGNDGDIDDGRDVCTMGSPAIAKNLLAIGSTSSGETRLTKTTPDGEASRDFNGGADIDTVSYFSSYGPTRDGRIKPELVAPGDMIYSAASDGTDRYSCRLWAYQGTSMSCPIVAGAAAMVRQYFVDDSFYATDVTTRGFCG-YGFVCDGFSPSSATVKALLINSANLMGGSSDPDDFRGFGRIHMEAGMPLGGEGGLVLFVADSANTNIAEQTMQQYIFGVDSDAGLDLRATISWIDPAATAFSEKQLIHNLDLTLIAPSGTTHTMWST-GIADANNVNERVIIDAADVENGTW 2715
            PSLS++E  L TTA   FDGH  A++ A +W  + LR K   N RAP+L C  Y +G   +  L+E LSP AVR  +++   GACF VTAS+ +A ++S  P  + L+S+ PFPSALK+APG+L+H         S  RLTTTHG  MR  NVEGL++EL+PG L  H+PE   F   LL+DL S S+DLH GNVWSDP  A  +H  T  GA R R+W  AA +V +LA A  TSP DICSW+S ++H A  ++L+VSG+DHLL    G  G  +EA EL +ACFMGLVSFL+NR EVLRV+PRH  RLLNA AR  IQ+AT+T APLTDAGLDGTG+VIQV+D+GLDETSCFFIDDDGEEV HGHY+E L                                      +II   S  + + E                              GHGTH AGSA GAT+ TPAET  TCS  +V GCVG CI        DDL TL  ++  Y  D+DRLCP  DC    +  CLS DV +TLA HGGMAQGAKLA FD+F          GNG+WE C EAGCK+HS S GAD+ C + E DV YDDFMYNNPENL++ +AGN GD +DGR VCTM SPAI KN LA+G+TSSGETR+TKT+ DG+     NG AD+DTV+ FSSYGPT+DGRIKPE+VAPGD IYSA SDG+D +SCRL A  GTSM+CPIVAGA+AMVRQYF+D+SFY  D   RG+    G  C+ FSPSSATVKALLINSANLMGGSS+PD +RGFGRIH+E GMPL GEG L LFVADS+ T++ E   Q +IF VD++A LD RAT+SWIDP A++ S  QL+H+LDL +++PSG TH MW+T G  D+ NVNERVII A DVE+GT+
Sbjct:   32 PSLSVVEGSLLTTAQDSFDGHAKAFETAAQWTGEILRSKEEDNARAPHLVCTEYNRGGKTLPRLQELLSPGAVRPVAHSSTHGACFFVTASHAEARAMSDSPY-YGLTSISPFPSALKVAPGVLEHGVSLDQASQSPGRLTTTHGHLMRKGNVEGLNMELSPGTLPAHSPEAGMFIAELLDDLMSDSIDLHGGNVWSDPVAANGEHLTTPAGAVRKREWSMAATLVHQLAVAGRTSPGDICSWDSISMHYAGNDLLLVSGLDHLLHKENGAIGSDEEATELRVACFMGLVSFLSNRLEVLRVSPRHADRLLNAVARTIIQSATITEAPLTDAGLDGTGQVIQVIDTGLDETSCFFIDDDGEEVPHGHYYEALG-------------------------------------EIIPSSSSARDDVE------------------------------GHGTHCAGSALGATIRTPAETPTTCSGTEVPGCVGGCIDADRTSWGDDLFTLMPEYSPYDIDIDRLCPMIDCDDATDPRCLSDDVGQTLADHGGMAQGAKLAFFDVFADGSSGTHFVGNGLWEPCLEAGCKIHSMSIGADFECSVCEKDVLYDDFMYNNPENLVVVAAGNAGDTEDGRTVCTMYSPAIGKNALAVGATSSGETRITKTSADGDLIDGTNGYADVDTVAQFSSYGPTQDGRIKPEVVAPGDAIYSARSDGSDNHSCRLSAKSGTSMACPIVAGASAMVRQYFMDESFYVADQAARGWSDDSGRRCESFSPSSATVKALLINSANLMGGSSEPDGYRGFGRIHLEMGMPLDGEGSLALFVADSSYTSLPELCSQDFIFDVDANANLDFRATLSWIDPPASSASGVQLVHDLDLEVVSPSGATHIMWATSGEVDSRNVNERVIIAAEDVESGTY 878          

BLAST of mRNA_F-serratus_M_contig646.17401.1 vs. uniprot
Match: A0A2I6U1S5_SACJA (Subtilisin n=1 Tax=Saccharina japonica TaxID=88149 RepID=A0A2I6U1S5_SACJA)

HSP 1 Score: 904 bits (2337), Expect = 2.700e-316
Identity = 495/939 (52.72%), Postives = 622/939 (66.24%), Query Frame = 1

Query:    1 PSLSLLESVLRTTAIHDFDGHDPAYQAAVRWDRQRLRDKFS-NQRAPYLACAGYGKGRGAMNGLKEFLSPEAVRTASNAVETGACFIVTASNEQAASISAHPEEFSLSSVGPFPSALKLAPGLLDH----KNDGNSSERLTTTHGERMRMANVEGLSVELAPGILGMHTPEVLPFAKSLLEDLASKSLDLHNGNVWSDPAMAGADHHLTEGGAWRARQWIRAADVVEELAEAVDTSPSDICSWESTAIHQASANVLVVSGVDHLLASGRGVGGQGKEALELHMACFMGLVSFLANRPEVLRVAPRHETRLLNAAARANIQTATVTSAPLTDAGLDGTGEVIQVVDSGLDETSCFFIDDDGEEVEHGHYFEELAVFRVGFLTASSYDVYSSYTYVHGVYEGGDFSYDTSRRKIIQYISLVKSESEPSSHGEYYTTESGIQIPWVDAASFEQDYAAGHGTHTAGSAAGATLNTPAET-VTCSSPDVLGCVGACISDSAAYSYDDLVTL--SSQFYFADLDRLCPSFDCGGYDEEVCLSGDVDETLAKHGGMAQGAKLAIFDMFYGEFGFGSLAGNGVWEACSEAGCKVHSNSYGADYLCEMSEGDVEYDDFMYNNPENLLIFSAGNDGDIDDGRDVCTMGSPAIAKNLLAIGSTSSGETRLTKTTPDGEASRDFNGGADIDTVSYFSSYGPTRDGRIKPELVAPGDMIYSAASDGTDRYSCRLWAYQGTSMSCPIVAGAAAMVRQYFVDDSFYATDVTTRGFCG-YGFVCDGFSPSSATVKALLINSANLMGGSSDPDDFRGFGRIHMEAGMPLGGEGGLVLFVADSANTNIAEQTMQQYIFGVDSDAGLDLRATISWIDPAATAFSEKQLIHNLDLTLIAPSGTTHTMWST-GIADANNVNERVIIDAADVENGTWAIRVWANELTTDVQSYSLVVNGAI 2787
            PSL ++E  L TTA   FDGH   ++ A +W    +R K   N RAP+L C  Y +G   ++ L+E LSPEAVR+ +++   GACF VTAS+ +A ++S +P  + L+S+ PFPSALK+APG+L+H         S  RL TTHG  MR  NVEGL++EL+PG L  H+P+   F   LL+DL S S+DLH GNVWSDP  A  +H  T  GA R R+W  AA +V +LA A  TSP DICSW+S +IH A  ++L+VSG+DHLL    G  G  +EA EL +ACFMGLVSFL++R EV RV+PRH  RLLNA AR  IQ+AT+T APLT AGLDGTG+VIQV                                                                     IQYI+L+K++S         TT  G ++ WV    F+ D    HGTHTAGSA GAT+ TPAET  TCS  +V GCVG CI        DDL+TL   +  Y AD+DR+CP   C   +E  CLS  V++TLA HGGMAQGAKL+ FD++ G   F    GNG+WE C EAGCK+HS S+GAD  C + + D+ YDDFMYNNPENL++ +AGN G+I+DGR VCTM SPAI KN LA+G+TSSGETR+  T+ DG+ +   NG +D+DTV+ FSSYGPT+DGRIKPE+VAPGD +YSA SDG+D +SCRL A  GTSM+CPIVAGA+AMVRQYF+D+SFY  D   RG+C   G  C+ FSPSSATVKALLINSANLMGGSS+PD FRGFGRIH+E GMPL GEG L LFVADS+ T++ E + Q ++F VD++A LD RAT+SWIDP A++ S  QL+H+LDL +++PSG TH MW+T G  D+ NVNERVII A +VE+GT+ +RV  N L TD Q+YSLVVNGA+
Sbjct:   32 PSLGVVEGSLLTTAHESFDGHAKGFETAAQWTGDIIRSKEEDNARAPHLVCTEYNRGGETLSRLQELLSPEAVRSVAHSSTHGACFFVTASHAEARAMSDNPY-YGLTSISPFPSALKVAPGVLEHGVSRDQASQSPGRLATTHGHLMRKGNVEGLNLELSPGTLPAHSPQAGMFIAELLDDLMSDSIDLHGGNVWSDPVAANGEHLTTPAGAVRKREWSMAATLVHQLAVAGRTSPGDICSWDSISIHHAGNDLLLVSGLDHLLHKESGAIGSDEEATELRVACFMGLVSFLSSRLEVSRVSPRHADRLLNAVARTIIQSATITKAPLTAAGLDGTGQVIQV---------------------------------------------------------------------IQYINLIKADSVSGPASS--TTSGGNRVWWVSPDGFQGDDVDDHGTHTAGSALGATIRTPAETPTTCSGTEVPGCVGGCIDADRTSWGDDLLTLMPENSSYVADIDRICPMLGCDEENEPWCLSDGVEQTLADHGGMAQGAKLSFFDIYDGNMLFEQFIGNGLWEPCLEAGCKIHSLSFGADLECAVRDTDLLYDDFMYNNPENLVVVAAGNAGEIEDGRTVCTMYSPAIGKNALAVGATSSGETRVGVTSADGDRADGTNGYSDVDTVAVFSSYGPTQDGRIKPEVVAPGDAVYSARSDGSDVHSCRLSAKSGTSMACPIVAGASAMVRQYFMDESFYLADQAARGWCDDSGRQCESFSPSSATVKALLINSANLMGGSSEPDGFRGFGRIHLEMGMPLDGEGSLALFVADSSYTSLPELSSQDFVFDVDANANLDFRATLSWIDPPASSTSGVQLVHDLDLEVVSPSGATHIMWATSGEVDSRNVNERVIIAAENVESGTYTVRVSTNHLLTDSQNYSLVVNGAM 898          

BLAST of mRNA_F-serratus_M_contig646.17401.1 vs. uniprot
Match: D8LQI1_ECTSI (Subtilisin n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LQI1_ECTSI)

HSP 1 Score: 844 bits (2181), Expect = 5.710e-292
Identity = 480/959 (50.05%), Postives = 629/959 (65.59%), Query Frame = 1

Query:    1 PSLSLLESVLRTTAIHDFDGHDPAYQAAVRW---DRQRLRDKFSNQRAPYLACAGYGKGRGAMNGLKEFLSPEAVRTASNAVETGACFIVTASNEQAASISAHPEEFSLSSVGPFPSALKLAPGLLDHKN-DGN-------------SSERLTTTHGERMRMANVEGLSVELAPGILGMHT---PEVLPFAKSLLEDLASKSLDLHNGNVWSDPAMAGADHHLTEGGAWRARQWIRAADVVEELAEAVDTSPSDICSWESTAIHQASANVLVVSGVDHLLASGRGVGGQGKE-ALELHMACFMGLVSFLANRPEVLRVAPRHETRLLNAAARANIQTATVTSAPLTDAGLDGTGEVIQVVDSGLDETSCFFIDDDGEEVEHGHYFEELAVFRVGFLTASSYDVYSSYTYVHGVYEGGDFSYDTSRRKIIQYISLVKSES-EPSSHGEYYTTESGIQIPWVDAASFEQDYAAGHGTHTAGSAAGATLNTPAETVTCSS-PDVLGCVGACISDSAAYSYDDLVTLSSQFYFADLDRLCPSFDCGGYDEEVCLSGDVDETLAKHGGMAQGAKLAIFDMFYGEFGFGSLAGNGVWEACSEAGCKVHSNSYGADYLCEMSEGDVEYDDFMYNNPENLLIFSAGNDGDIDDGRDVCTMGSPAIAKNLLAIGSTSSGETRLTKTTPDGEASRDFNGGADIDTVSYFSSYGPTRDGRIKPELVAPGDMIYSAASDGTDRYSCRLWAYQGTSMSCPIVAGAAAMVRQYFVDDSFYATDVTTRGFCGYGFVCDGFSPSSATVKALLINSANLMGGSSDPDDFRGFGRIHMEAGMPLGGEGGLVLFVADSANTNIAEQTMQQYIFGVDSDAGLDLRATISWIDPAATAFSEKQLIHNLDLTLIAPSGTTHTMWSTGIADANNVNERVIIDAADVENGT----WAIRVWANELTT-DVQSYSLVVNGAISP 2793
            PSL   E  L TTA  DFD  + A+  A  W   DRQ        + +PYL+C  +G+GR A   L+ FL   AV+  S+    GACF+VTAS  +AA +S+  E+F+  + G FP+ALK+APGLLDH + DG              S+ RL+TTHG  M++ +  GL VEL+PG L  H+    +   F + LL+ L+S SLDLH    WSD  M G DH  +  GA R R+W RAA +V E +E+   +PS +C WE   +H A+ +VL+V+G+DHLL  GR V G  +E A EL MAC+MGLVSFLA+R EVL+VA  +    LNAAARA IQ+A+V S PLTDAGLDG+GEVIQ++D+G++ET+CF    DG EVEHG+YF+ +A     F ++S      S +     + GGDFSYD+ RRK+IQYI L+  ++ + SS G          +P+V A SF  D   GHGTH A + AG+T+++PAE V+C+   +V+GC G CI++S+  S       S   Y  D++RLCP+F C G   + CL  D  +TL++HGGMAQGAK+A  D+FYG++ +G LAGNG+WE C++AGCK+HSNSYG D  C+++  DV YD++MY N E+LL+FSAGN G   D RD CT+ SP IAKN+L +GSTSSG TRL+ T  DG    D +  + ID VS FS YG T DGRIKPE+VAPGDMIYSA+ +G D  SC L AY GTS S  + +GAAAMVRQYF D SFY+ DV +RGFC  GF CD FSP + TVKA+LINSAN+MGG ++PD  RGFGRIH+EAGMPLGG G + LFVADS++  +      ++ F VD DAG++LRAT+SW DPAA++ S  QL+++LDL + AP GT + MW     D +NVNERVI+ A  V   +    W +RV   + T  D  S+SLVV GAISP
Sbjct:   25 PSLGFFEQTLLTTAGADFDS-EAAFAFASAWQAGDRQ-------GRSSPYLSCVEHGEGRHAYRKLEGFLGARAVKPVSSTATHGACFMVTASPAEAAQLSSW-EDFT--TFGAFPAALKIAPGLLDHGSCDGPAAARDAATTTSDCSAGRLSTTHGASMQVDSAHGLMVELSPGTLPSHSGGDAKAHEFIQELLKSLSSPSLDLHAITCWSDLVMKGGDHLFSAAGALRGREWSRAATLVNERSESEQETPSQVCGWEQVVVHHAANDVLLVAGLDHLLYDGREVSGASEEEAAELRMACYMGLVSFLASRHEVLKVAGWNRKVNLNAAARATIQSASVDSTPLTDAGLDGSGEVIQIIDAGVNETTCFLEHGDGTEVEHGYYFDFVASSNPLFTSSSE-----SRSMFKAEFTGGDFSYDSDRRKVIQYIDLINPDTGDGSSSG----------MPFVSADSFVSDDDLGHGTHAATTVAGSTISSPAELVSCADGSEVIGCAGGCITESSTGS-------SEADYGEDINRLCPAFGCSG---DQCLPDDTADTLSEHGGMAQGAKIAAIDVFYGDYSYGDLAGNGLWETCADAGCKIHSNSYGVDLRCQLTALDVLYDEYMYENQEHLLLFSAGNTGWTTD-RDTCTIDSPGIAKNVLTVGSTSSGVTRLSTTDLDGRGYADGDELSTIDVVSEFSGYGFTEDGRIKPEVVAPGDMIYSASGNGGDS-SCELIAYAGTSFSTAVASGAAAMVRQYFRDSSFYSADVESRGFCDQGFTCDSFSPMATTVKAMLINSANVMGGGNEPDTERGFGRIHLEAGMPLGGAGDMALFVADSSDHEVYSDEKLKFYFNVDPDAGMELRATLSWNDPAASSLSGDQLVNDLDLRVYAPDGTRYCMWDDNCWDTSNVNERVIVPAEVVAQFSATDEWEVRVRGRDFTVGDTVSFSLVVTGAISP 945          

BLAST of mRNA_F-serratus_M_contig646.17401.1 vs. uniprot
Match: D8LNG4_ECTSI (Subtilisin n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LNG4_ECTSI)

HSP 1 Score: 786 bits (2029), Expect = 1.270e-272
Identity = 405/678 (59.73%), Postives = 501/678 (73.89%), Query Frame = 1

Query:  844 MGLVSFLANRPEVLRVAPRHETRLLNAAARANIQTATVTSAPLTDAGLDGTGEVIQVVDSGLDETSCFFI-DDDGEEVEHGHYFEELAVFRVGFLTASSYDV--------------------YSSYTYVHGVYEGGDFSYDTSRRKIIQYISLVKSESEPSSHGEYYTTESGIQIPWVDAASFEQDYAAGHGTHTAGSAAGATLNTPAETVTCSSPDVLGCVGACISDSAAYSYDDLVTLSSQFYFADLDRLCPSF---DCGGYDEEVCLSGDVDETLAKHGGMAQGAKLAIFDMFYGEFGFGSLAGNGVWEACSEAGCKVHSNSYGADYLCEMSEGDVEYDDFMYNNPENLLIFSAGNDGDIDDGRDVCTMGSPAIAKNLLAIGSTSSGETRLTKTTPDGEASRDFNGGADIDTVSYFSSYGPTRDGRIKPELVAPGDMIYSAASDGT-DRYSCRLWAYQGTSMSCPIVAGAAAMVRQYFVDDSFYATDVTTRGFCGYGFVCDGFSPSSATVKALLINSANLMGGSSDPDDFRGFGRIHMEAGMPLGGEGGLVLFVADSANTNIAEQTMQQYIFGVDSDAGLDLRATISWIDPAATAFSEKQLIHNLDLTLIAPSGTTHTMWSTGIADANNVNERVIIDAADVENGTWAIRVWANELTTDVQSYSLVVNGAISPATG 2802
            MGLVSFLA++PEVLRVAPR    LLNAAARANIQ+ATVT  PLTDAGLDGTG+V+QV+DSG+DE+SCFF  D+ G+ V HGHYFEE  +     LT+SS+                        SY  +   ++GGDF+    RRKIIQYI+L+KS+    SHG+ +TT  G Q PW+ A  FE D  AGHGTH AGS AGATLNTPAE ++C+  + + CVGAC+ D  A   DDL T   +   ADLDRLCP F   DC G + + CLS DV ETL  +GGMAQGAKLAIFD FY  FG     G G+WE C++A CK+HSNSYG D+ C +   DV YD++M +NPENLLIF+AGNDG++   R  CT+GSPAIAKN+LA+G+++SG+TRL+ T            G+ IDTV+ FSSYGPT D RIKPE++APGD IYSAASDG+ D +SCRLWAY GTSMS PIVAGA+AM+RQYF++++F+ATD+T RGFC     C+ FSPSSAT+KAL+INSANLMG SS+P+  RGFGRIH+EAGMPLGGEG L LFV+DS  T+I E T Q+Y F V++ AGLDLR T+SW+DP AT FS  QL+H+LDL +I+P+GT +TMW++G+ D++NVNERVI+DA DVE GTW++ +WA+ LTTDVQSYSLVVNGAISP TG
Sbjct:    1 MGLVSFLASKPEVLRVAPRQSQALLNAAARANIQSATVTDTPLTDAGLDGTGQVVQVIDSGVDESSCFFAHDESGDHVPHGHYFEEWGINPS--LTSSSFSSPXXXXXXXXXXXPWATADHHTGSYYQIAEEFDGGDFTIYPDRRKIIQYINLIKSDDTEGSHGDSFTTSLGQQYPWLSADHFEYDDEAGHGTHVAGSVAGATLNTPAELISCNDTETMSCVGACVDDDEANDVDDLATPYVEI--ADLDRLCPLFSDYDCDGLETDKCLSDDVAETLTNNGGMAQGAKLAIFDAFYDTFGLSPFVGTGLWEPCADADCKIHSNSYGGDFECSLGSNDVLYDEWMVSNPENLLIFAAGNDGELR--RSTCTIGSPAIAKNVLAVGASTSGDTRLSATP-----------GSGIDTVAVFSSYGPTSDSRIKPEVLAPGDAIYSAASDGSVDTHSCRLWAYVGTSMSTPIVAGASAMIRQYFMNETFFATDMTARGFCDEWAGCEAFSPSSATLKALMINSANLMGESSEPNGSRGFGRIHLEAGMPLGGEGSLALFVSDSNYTSIPELTRQEYNFDVNATAGLDLRVTLSWLDPPATTFSAVQLVHDLDLVVISPNGTRYTMWASGVTDSSNVNERVIVDADDVETGTWSVWIWADNLTTDVQSYSLVVNGAISPGTG 661          

BLAST of mRNA_F-serratus_M_contig646.17401.1 vs. uniprot
Match: A0A6H5K365_9PHAE (Subtilisin n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5K365_9PHAE)

HSP 1 Score: 780 bits (2014), Expect = 8.500e-271
Identity = 411/690 (59.57%), Postives = 500/690 (72.46%), Query Frame = 1

Query:  760 SGVDHLLASGRGVGGQGKE-ALELHMACFMGLVSFLANRPEVLRVAPRHETRLLNAAARANIQTATVTSAPLTDAGLDGTGEVIQVVDSGLDETSCFFIDDDGEEVEHGHYFEELAVFRVGFLTASSYDVYSSYTYVHGVYEGGDFSYDTSRRKIIQYISLVKSESEPSSHGEYYTTESGIQIPWVDAASFEQDYAAGHG--------THTAGSAAGATLNTPAETVTCSSPDVLGCVGACISDSAAYSYDDLVTLSSQFYFADLDRLCPSFDCGGYDEEVCLSGDVDETLAKHGGMAQGAKLAIFDMFYGEFGFGSLAGNGVWEACSEAGCKVHSNSYGADYLCEMSEGDVEYDDFMYNNPENLLIFSAGNDGDIDDGRDVCTMGSPAIAKNLLAIGSTSSGETRLTKTTPDGEASRDFNGGADIDTVSYFSSYGPTRDGRIKPELVAPGDMIYSAASDGTDRYSCRLWAYQGTSMSCPIVAGAAAMVRQYFVDDSFYATDVTTRGFCGYGFVCDGFSPSSATVKALLINSANLMGGSSDPDDFRGFGRIHMEAGMPLGGEGGLVLFVADSANTNIAEQTMQQYIFGVDSDAGLDLRATISWIDPAATAFSEKQLIHNLDLTLIAPSGTTHTMWSTGIADANNVNERVIIDAADVENGTWAIRVWANELTTDVQSYSLVVNGAISPATG 2802
            +G+DHLL SGRG GG  +E + ELH+ACFMGLVS LA RPEVLRV+PR   R+LNAAAR  +Q+A+ T  PLTDAGLDGTGE+IQV+DSGLDETSC+FIDD GEEV+HG+YF+ELA        A+ Y    S      ++ GGDFSY   RRKIIQYI LV  +S  SS   +YT++      ++    F +D  A                SAAGATLN PAET+TC+  D L CVGACI        DDLV++S Q    DLDRLCP + C    +  CLS DV ETL+ +GGMAQGAKLAIFD+FYG++   S  GN +WE C+EAGCK+HSNS GAD+ C ++  D+  D FMY N E+LLIF+AGN+G   + R  CT+ +P I KN LAIG+++SG+TR+  T           G   I+TV+ FSS+G T D RIKPE+VAPGD +YSAASDGTD +SCRLWAY+GTSMSCPIVAGAAAM+RQYF+D++FY  DVT RGFC   F+C+GFSPS+ATVKALLINSANLMGGSS+PD FRGFGR+H+EAGMPL GEG LVLFVAD+ +T+IAE T+ +Y+F VD+DAGLDLRAT+SWIDP +TA S  QL+HNLDL +++P GTT+ MW +   D  NVNERVI+DA DV  GTW + VWAN L TD QSYSLVVNGAISP TG
Sbjct:    4 TGLDHLLFSGRGAGGSHEEESTELHVACFMGLVSVLAGRPEVLRVSPRPTKRVLNAAARGIVQSASATDTPLTDAGLDGTGEIIQVIDSGLDETSCYFIDDSGEEVDHGYYFDELA-------QAAVYSSLYSSDETATIFNGGDFSYYPERRKIIQYIDLVNPDSPYSS---FYTSQGSYIGYFLPPDQFGEDDEARVALRKKDPSQARVGRSAAGATLNNPAETITCNVTDTLSCVGACID--LLDVTDDLVSISHQTGGTDLDRLCPMYGCDNSTDTTCLSDDVPETLSNNGGMAQGAKLAIFDVFYGDYDASSSIGNNLWEVCTEAGCKLHSNSLGADFECSIASWDILNDQFMYENAEHLLIFAAGNEG--GEERSTCTINTPGIGKNSLAIGASTSGDTRVISTV----------GATGINTVAEFSSWGFTTDDRIKPEVVAPGDSVYSAASDGTDTHSCRLWAYEGTSMSCPIVAGAAAMIRQYFMDETFYMADVTARGFCSDVFICEGFSPSAATVKALLINSANLMGGSSEPDGFRGFGRVHLEAGMPLEGEGNLVLFVADALDTSIAEGTLDEYLFDVDADAGLDLRATLSWIDPPSTATSAVQLVHNLDLVVVSPDGTTYRMWGSETTDNRNVNERVIVDATDVVTGTWTVWVWANSLITDSQSYSLVVNGAISPGTG 669          

BLAST of mRNA_F-serratus_M_contig646.17401.1 vs. uniprot
Match: A0A6H5JKA8_9PHAE (Subtilisin n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JKA8_9PHAE)

HSP 1 Score: 792 bits (2046), Expect = 9.960e-270
Identity = 486/1065 (45.63%), Postives = 624/1065 (58.59%), Query Frame = 1

Query:    1 PSLSLLESVLRTTAIHDFDGHDPAYQAAVRWDRQRLRDKFSNQRAPYLACAGYGKGRGAMNG-LKEFLSPEAV--RTASNAVETGACFIVTASNEQAASISAHPEEFSLSSVGPFPSALKLAPGLLDHKND----GNSSERLTTTHGERMRM----------------------------------------------ANVEGLSVELAPGIL----------GMHTPEVLPFAKSLLEDLASKSLDLHNGNVWS--------DPAMAGADHHLTEGGAWR--ARQWIRAADVVEELAEAVDTSPS--DICSWESTAIHQASANVLVVSGVDHLLASGRGVGG-QGKEALELHMACFMGLVSFLANRPEVLRVAPRHETRLLNAAARANIQTATVTSAPLTDAGLDGTGEVIQVVDSGLDETSCFFIDDDGEE-VEHGHYFEELAVFRVGFLTASSYDVYSSYTYVHGVYEGGDFSYDTSRRKIIQYISLVKSESEPS--------------------------SHGEYYTTESGIQIPWVDAASFEQDYAAGHGTHTAGSAAGATLNTPAETVT------CSSPDVLGCVGACISDSAAYSYDDLVTLSSQFYF------ADLDRLCPSFDCGGYDEEVCLSGDVDETLAKHGGMAQGAKLAIFDMFYGEFGFG-SLAGNGVWEACSEAGCKVHSNSYGADYLCEMSEGDVEYDDFMYNNPENLLIFSAGNDGDIDDGRDVCTMGSPAIAKNLLAIGSTSSGETRLTKTTPDGEASRDFNGGADIDTVSYFSSYGPTRDGRIKPELVAPGDMIYSAASDGTDRYSCRLWAYQGTSMSCPIVAGAAAMVRQYFVDDSFYATDVTTRGFCGYGFVCDGFSPSSATVKALLINSANLMGGSSDPDDFRGFGRIHMEAGMPLGGEGGLVLFVADSANTNIAEQTMQQYIFGVDSDAGLDLRATISWIDPAATAFSEKQLIHNLDLTLIAPSGTTHTMWSTGIADANNVNERVIIDAADV---------------ENGTWAIRVWANELT-TDVQSYSLVVNGAISPAT 2799
            P LS++   L TTA   F  H   ++AAV W   RL D    + APYLACA     RG     L   L   A+  R   ++ E G C + TA+  QA SI    + FS  S   FPSALKLAPGLLDH +D    G    RL  THGER+R                                               + V GL+VELAPG+L          G              E+L S+SLD+H+ + WS        D A A      + GG     AR+W RAADV+  L    +  P+  D+CSW+   +     ++L + G+DHLL  GRG GG +G EA EL +ACFM L+SFLA+RPEVLRV+P    +LLNAAA  N+Q+A++T +P+TDAGLDGTGEVIQ+VD+GLD  SCFF+D+DG E V HG+YFEE+ V    F   S +  +     +  V+ GGDF++D SRRK +   +   S   P+                          ++GE + T +G Q PW+ AA F +D A GHGTHTAGSAAGA +++P+ + T      C     L CVG CI     +  DDL+T     YF       DLDRLCP  DC    EE CL  D  ETL +HGG+A+GA+LAIFD+     G G  +AGNG+WE   EAG K+ SNS+G    C+    DV +DDFMY N + LL+F+AGNDGD   G   CTMG+PA+AKN+LA+G+++SG  RL+ T  DG+ +   +   DID ++YFSS+GPT D RIKPE+VAPGDMIYSAA  G   Y+CRLW Y GTSMS PIVAGAAA+VRQYF +++FYA DV  RGFC   F C+ F+PS+ATVKA+LINSANLMGGS++PD  RGFGR+H+EAGMPL GEG   LFVADSA T+ AE T+ +Y F VD+ AG+DLRAT+SWIDP AT FS  QL+H+LDL++ +P+G  ++MW  G  D+ N NERVI+ A+++                +GTW +RVW+  L   + QSYSLVV GAI P T
Sbjct:   30 PPLSVIRGALLTTAGVTFR-HQGEFKAAVEWHEARLDDA---RPAPYLACASPDSDRGGSGATLSHVLGAGAIGGRVVVSSAEHGVCVVATATPGQAESIFQGQQVFS--SWAAFPSALKLAPGLLDHPHDDRDGGGGDGRLRMTHGERLRRPKDRKGXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXDNIGGGPSGVTGLTVELAPGVLAATNRRGSSGGGWYASSGELTDEWREELMSQSLDVHDKSFWSSGWSAKLPDEAAAEGAGDSSGGGPGEVLAREWTRAADVLRGLG-GTEGGPTIGDVCSWDGLVVVHTDDDLLTMKGMDHLLPEGRGAGGNEGPEADELKVACFMALLSFLASRPEVLRVSPARAIKLLNAAAYHNVQSASLTESPMTDAGLDGTGEVIQIVDTGLDVNSCFFVDEDGGEGVAHGYYFEEIGVEASSF--GSWFTSWDFNVQIARVFTGGDFNFDLSRRKGLVVDAAHPSCDSPNISRRFALGDVRAWSGENPACTANNSTNYGESWATSAGEQYPWISAAEFAEDDAGGHGTHTAGSAAGAVVSSPSYSQTGDFTEVCEEGRELSCVGGCIDVDDPFGEDDLITTWDYQYFWAGYEAIDLDRLCPRLDCDDLPEEYCLGDDEAETLQQHGGVARGAQLAIFDIINDYTGVGIDMAGNGMWEPAMEAGSKLSSNSWGIYAYCDYLSLDVLFDDFMYRNQDQLLLFAAGNDGDDWSG---CTMGTPALAKNVLAVGASTSGPGRLSFTDEDGDPTDGDSRVQDIDAMAYFSSFGPTLDNRIKPEVVAPGDMIYSAAGSGDGNYTCRLWQYAGTSMSTPIVAGAAALVRQYFKNETFYAHDVEERGFCD-SFGCEPFAPSAATVKAMLINSANLMGGSAEPDGLRGFGRVHLEAGMPLAGEGDNALFVADSAVTSTAELTLTEYSFAVDAAAGIDLRATLSWIDPPATTFSSVQLVHDLDLSVYSPTGLRYSMWRDGTVDSLNNNERVIVPASNILDQTSSDATTAEDGESDGTWTVRVWSKRLAGNETQSYSLVVTGAIGPPT 1081          

BLAST of mRNA_F-serratus_M_contig646.17401.1 vs. uniprot
Match: D8LSW0_ECTSI (Subtilisin n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LSW0_ECTSI)

HSP 1 Score: 681 bits (1757), Expect = 5.300e-228
Identity = 437/1039 (42.06%), Postives = 580/1039 (55.82%), Query Frame = 1

Query:    7 LSLLESVLRTTAIHDFDGHDPAYQAAVRWDRQRLR-DKFSNQRAPYLACAGYGKGRGAMNGLKEFLSPEAVRTASNAVETGACFIVTASNEQAASISAHPEEFSLSSVGPFPSALKLAPGLLDHKND-------------GNSSERLTTTHGERMRMANVEGLSVELAPGILGMHTPEVLPFAKSLLED----LASKSLDLHNGNVWSDPAMAGADHHLTEGGAWRARQWIRAADVVEELAEAVDTSPSDICSWESTAIHQASANVLVVSGVDHLLASGRGVGGQGKEALELHMACFMGLVSFLANRPEVLRVAPRHETRLLNAAARANIQTATVTSAPLTDAGLDGTGEVIQVVDSGLDETSCFFIDDDGEEVEHGHYFEELAVFRVGFLTASSYDVYSSYTYVHGVYEGGDFSYDTSRRKIIQYISLVKSESEPSSH-------------------------------GEYYT---TESGIQIPWVDAAS------------------------FEQDYAAGHGTHTAGSAAGA-TLNTPAETVTCSSPDVLGCVGACISDSAAYSYDDLVTLSSQFYFADLDRLCPSFDCGGYDE---EVCLSGDVDETLAKHGGMAQGAKLAIFDMFY-GEFGFGSLAGNGVWEACSEAGCKVHSNSYGADYLCEMSEGDVEYDDFMYNNPENLLIFSAGNDGDIDD--GRDVCTMGSPAIAKNLLAIGSTSSGETRLTKTTPDGEASRDFNGGAD--------------------------IDTVSYFSSYGPTRDGRIKPELVAPGDMIYSAASDGTDRYSCRLWAYQGTSMSCPIVAGAAAMVRQYFVDDSFYATDVTTRGFCGY---GFVCDGFSPSSATVKALLINSANLMGGSSDPDDFRGFGRIHMEAGMPLGGEGGLVLFVADSANTNIAEQTMQQYIFGVDSDAGLDLRATISWIDPAATAFSEKQLIHNLDLTLIAPSGTTHTMWSTGIADANNVNERVIIDAADVENGTWAIRVWANELTTDVQSYSLVVNGAI 2787
            L  ++++  ++A  DF     AY AA  W   RL  D      APY+ACA Y +GR A+  L+   +  AV   SN    G CFIVTA    A ++   PE+F L S  P   +LKLA  LLDH  D              + S  L +T+GE M + NV GLSV L+PG+L     E   FA++ L D    L S+S+++   + WSD     +++ +T     R R+W RAA VV+ LA     +  +IC +    +   + ++++V G++HLL   R          E   AC+MGL+S LA +PEVLRV+P    RL NA A A +Q+AT T  PL DAGLDGTGEVIQVVDSGLDETSCFF DDDG +VEHG  F+ + V   G ++            V G Y    F +D SRRKI+QYI L K+  + S+                                GEYY+   +E       +D  S                        F +D   GHGT TAGSAAGA +  +  E   C   ++ GC G CIS S      D +   S F   DLD  CP + C G  +     CLS D  E L +HGG+A GA++++FD+ Y G   + S AGN +W +  E G K+H+NS+G   LC+++E +  YD FMY NPE+LLIF+AGNDG  +D   R+ CT+GSPA+ KN LA+G+TSSG    T+T  DG    +  G  D                          +DTV++FSS GPT DGRIKP++VAPGD + SA+SDGTD +SC+L A  GTS SCP+VAGAAA+VRQYF D SF+A DV +RG C        C  F+PS+ATVKA++INSA+LMGGSS+P+  RGFGR+H+EAGMP+ G G   L V ++A   IA+  + + +  VD DAG++LRAT++W+DP  TA S KQL H+LDL +IAPSG  +TMW +G+ D  NV ERVI+    +E+G W++ V A  L TD Q YSLVV GAI
Sbjct:   14 LGKIQAITASSAFDDFARSQSAYSAASSWYNDRLSYDAAEGSTAPYVACADYKQGRHALTSLETAFTKSAVHRVSNTEADGTCFIVTALPSSATAVIRAPEDFDLLSAAPLLPSLKLATSLLDHGPDTSPRLGAADDFDLSDHSAPLRSTYGEGMTLDNVHGLSVRLSPGVLTAGGRE--SFAEAFLRDWHADLTSESVNMRRASFWSDSDADRSEYDVT-----RVREWSRAAAVVDGLASKSARTVGEICGFGKLKLRHVADDLVLVEGMNHLLPGKR-------FPSETRTACYMGLLSQLAAKPEVLRVSPLQSARLHNAVANALVQSATTTETPLLDAGLDGTGEVIQVVDSGLDETSCFFADDDGLQVEHGFLFDGVRVEDDGNVST-----------VLGNYS---FPFDMSRRKIVQYIQLFKTPDQGSTSSGQDSFTYVSDYLGDPYDYDDSEYISYDDYSGEYYSDTWSEDSFCAGVLDDLSYHFSYLFDDTSYSSSSTSPSFTGGFHKDTVGGHGTWTAGSAAGAISAQSILEEEACYGDELPGCAGGCISASDV----DAMLDDSTF---DLDVFCPMYTCDGNTDIPSSECLSDDPVENLHQHGGVAPGAQISVFDVSYVGTEIYASFAGNDLWYSAMETGAKIHTNSWGGATLCQLTEDEYLYDTFMYENPEHLLIFAAGNDGGYEDIPNREACTVGSPALGKNSLAVGATSSGPFPGTRTGDDGNLVYEELGLTDYSPEGYPWICLTPFLGTPSASTEPAGVDTVAFFSSQGPTLDGRIKPDVVAPGDQVASASSDGTDGHSCKLVASMGTSASCPLVAGAAALVRQYFKDPSFFAKDVVSRGGCAEHSSSCQCSEFAPSAATVKAMIINSAHLMGGSSEPNGVRGFGRVHLEAGMPMDGVGETGLSVVEAA---IADGEITELVT-VDGDAGVELRATLAWLDPPTTALSAKQLQHDLDLHVIAPSGAKYTMWESGVDDTVNVVERVIVPVESMESGVWSVVVSAKALLTDEQLYSLVVTGAI 1013