mRNA_F-serratus_M_contig681.17869.1 (mRNA) Fucus serratus male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_F-serratus_M_contig681.17869.1
Unique NamemRNA_F-serratus_M_contig681.17869.1
TypemRNA
OrganismFucus serratus male (Fucus serratus male (Toothed wrack or serrated wrack))
Homology
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BLAST of mRNA_F-serratus_M_contig681.17869.1 vs. uniprot
Match: A0A6H5K499_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5K499_9PHAE)

HSP 1 Score: 85.9 bits (211), Expect = 2.120e-20
Identity = 45/65 (69.23%), Postives = 55/65 (84.62%), Query Frame = 1 
Query:    1 CRDKILETDVTSFDIFEDARKEVLHVMETNFKREFTETEGFRRIVDATEREQQELRLLRERGMMP 195
            CR++IL TDVT++DIF+ AR EVL VMETNF+REF ETEGFRRIVDA E+EQ+E RLL+  G +P
Sbjct:   27 CREEILSTDVTAYDIFDRARAEVLAVMETNFQREFVETEGFRRIVDAAEQEQRENRLLQAGGFLP 91
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BLAST of mRNA_F-serratus_M_contig681.17869.1 vs. uniprot
Match: D7FU02_ECTSI (Signal transducer, putative n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FU02_ECTSI)

HSP 1 Score: 85.9 bits (211), Expect = 1.400e-19
Identity = 46/65 (70.77%), Postives = 54/65 (83.08%), Query Frame = 1 
Query:    1 CRDKILETDVTSFDIFEDARKEVLHVMETNFKREFTETEGFRRIVDATEREQQELRLLRERGMMP 195
            CR++IL TDVT++DIF+ AR EVL VMETNF+REF ETEGFRRIVDA E EQ+E RLLR  G +P
Sbjct:  101 CREEILSTDVTAYDIFDRARVEVLTVMETNFQREFVETEGFRRIVDAAELEQRENRLLRAGGFLP 165
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BLAST of mRNA_F-serratus_M_contig681.17869.1 vs. uniprot
Match: D7FIR7_ECTSI (RGS domain-containing protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FIR7_ECTSI)

HSP 1 Score: 88.6 bits (218), Expect = 4.660e-19
Identity = 45/65 (69.23%), Postives = 58/65 (89.23%), Query Frame = 1 
Query:    1 CRDKILETDVTSFDIFEDARKEVLHVMETNFKREFTETEGFRRIVDATEREQQELRLLRERGMMP 195
            CR+KIL TDVT+FDIF++AR EVL VMETNF+R+F  TEGFRRI++A+++EQ+ELRLLR  GM+P
Sbjct:  101 CREKILATDVTAFDIFDEARAEVLAVMETNFRRDFVTTEGFRRILNASKQEQRELRLLRAGGMLP 165
[Close]
BLAST of mRNA_F-serratus_M_contig681.17869.1 vs. uniprot
Match: D7FIR9_ECTSI (Uncharacterized protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FIR9_ECTSI)

HSP 1 Score: 87.4 bits (215), Expect = 1.140e-18
Identity = 46/64 (71.88%), Postives = 55/64 (85.94%), Query Frame = 1 
Query:    1 CRDKILETDVTSFDIFEDARKEVLHVMETNFKREFTETEGFRRIVDATEREQQELRLLRERGMM 192
            CR+KIL TDVT+FDIFE+AR EVL VMETNF+ +F  TEGFRRI+DA+E+EQQEL LLR  GM+
Sbjct:  194 CREKILATDVTAFDIFEEARAEVLVVMETNFQSDFVTTEGFRRIMDASEKEQQELSLLRSGGML 257
[Close]
BLAST of mRNA_F-serratus_M_contig681.17869.1 vs. uniprot
Match: D7FIS3_ECTSI (Similar to retinally abundant regulator of G-protein signaling hRGS-r n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FIS3_ECTSI)

HSP 1 Score: 86.7 bits (213), Expect = 2.200e-18
Identity = 45/65 (69.23%), Postives = 55/65 (84.62%), Query Frame = 1 
Query:    1 CRDKILETDVTSFDIFEDARKEVLHVMETNFKREFTETEGFRRIVDATEREQQELRLLRERGMMP 195
            CR+KIL TDVT++DIF++AR EVL VMETNF+REF  TEGFRRI DA+E E +E+RLLR  GM+P
Sbjct:  193 CREKILATDVTAYDIFDEARAEVLVVMETNFQREFVTTEGFRRIADASEVEHREIRLLRAGGMLP 257
[Close]
BLAST of mRNA_F-serratus_M_contig681.17869.1 vs. uniprot
Match: A0A6H5KDM3_9PHAE (RGS domain-containing protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5KDM3_9PHAE)

HSP 1 Score: 86.7 bits (213), Expect = 2.330e-18
Identity = 45/65 (69.23%), Postives = 55/65 (84.62%), Query Frame = 1 
Query:    1 CRDKILETDVTSFDIFEDARKEVLHVMETNFKREFTETEGFRRIVDATEREQQELRLLRERGMMP 195
            CR+KIL TDVT++DIF++AR EVL VMETNF+REF  TEGFRRI DA+E E +E+RLLR  GM+P
Sbjct:  224 CREKILATDVTAYDIFDEARAEVLVVMETNFQREFVTTEGFRRIADASEVEHREIRLLRAGGMLP 288
[Close]
BLAST of mRNA_F-serratus_M_contig681.17869.1 vs. uniprot
Match: A0A6H5K0N5_9PHAE (RGS domain-containing protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5K0N5_9PHAE)

HSP 1 Score: 85.9 bits (211), Expect = 3.410e-18
Identity = 44/64 (68.75%), Postives = 56/64 (87.50%), Query Frame = 1 
Query:    1 CRDKILETDVTSFDIFEDARKEVLHVMETNFKREFTETEGFRRIVDATEREQQELRLLRERGMM 192
            CR++IL TDVT++DIF+ AR EVL VMETNF+R+F ETEGFRRIVDA+E EQ+E+RLLR  G++
Sbjct:  265 CREEILSTDVTAYDIFDRARAEVLAVMETNFQRDFVETEGFRRIVDASELEQREMRLLRAEGVL 328
[Close]
BLAST of mRNA_F-serratus_M_contig681.17869.1 vs. uniprot
Match: A0A6H5KAT5_9PHAE (RGS domain-containing protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5KAT5_9PHAE)

HSP 1 Score: 85.9 bits (211), Expect = 4.730e-18
Identity = 44/64 (68.75%), Postives = 57/64 (89.06%), Query Frame = 1 
Query:    1 CRDKILETDVTSFDIFEDARKEVLHVMETNFKREFTETEGFRRIVDATEREQQELRLLRERGMM 192
            CR+KIL TDVT+FDIF++AR EVL VMETNF+R+F  TEGFRRI++A+++EQ+ELRLLR  GM+
Sbjct:  346 CREKILATDVTAFDIFDEARAEVLAVMETNFRRDFVTTEGFRRILNASKQEQRELRLLRAGGML 409
[Close]
BLAST of mRNA_F-serratus_M_contig681.17869.1 vs. uniprot
Match: D7FTZ7_ECTSI (Similar to regulator of G-protein signaling 16 n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FTZ7_ECTSI)

HSP 1 Score: 84.3 bits (207), Expect = 1.450e-17
Identity = 43/64 (67.19%), Postives = 56/64 (87.50%), Query Frame = 1 
Query:    1 CRDKILETDVTSFDIFEDARKEVLHVMETNFKREFTETEGFRRIVDATEREQQELRLLRERGMM 192
            CR++IL TDVT++DIF+ AR EVL VMETNF+R+F ETEGFRRIVDA+E EQ+++RLLR  G++
Sbjct:  316 CREEILSTDVTAYDIFDRARAEVLAVMETNFQRDFIETEGFRRIVDASELEQRQMRLLRAEGVL 379
[Close]
BLAST of mRNA_F-serratus_M_contig681.17869.1 vs. uniprot
Match: D7FU07_ECTSI (Signal transducer, putative n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FU07_ECTSI)

HSP 1 Score: 81.6 bits (200), Expect = 1.550e-17
Identity = 41/64 (64.06%), Postives = 54/64 (84.38%), Query Frame = 1 
Query:    1 CRDKILETDVTSFDIFEDARKEVLHVMETNFKREFTETEGFRRIVDATEREQQELRLLRERGMM 192
            CR++IL TDVT++DIF+ AR EVL +METNF+++F ETEGFRRI DA+  EQQE+RLLR+ G +
Sbjct:  111 CREEILSTDVTAYDIFDHARAEVLALMETNFQQDFVETEGFRRIADASALEQQEIRLLRKEGFL 174
The following BLAST results are available for this feature:
BLAST of mRNA_F-serratus_M_contig681.17869.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Fucus serratus MALE vs UniRef90)
Total hits: 15
Match NameE-valueIdentityDescription
A0A6H5K499_9PHAE2.120e-2069.23Uncharacterized protein n=1 Tax=Ectocarpus sp. CCA... [more]
D7FU02_ECTSI1.400e-1970.77Signal transducer, putative n=1 Tax=Ectocarpus sil... [more]
D7FIR7_ECTSI4.660e-1969.23RGS domain-containing protein n=1 Tax=Ectocarpus s... [more]
D7FIR9_ECTSI1.140e-1871.88Uncharacterized protein n=1 Tax=Ectocarpus silicul... [more]
D7FIS3_ECTSI2.200e-1869.23Similar to retinally abundant regulator of G-prote... [more]
A0A6H5KDM3_9PHAE2.330e-1869.23RGS domain-containing protein n=1 Tax=Ectocarpus s... [more]
A0A6H5K0N5_9PHAE3.410e-1868.75RGS domain-containing protein n=1 Tax=Ectocarpus s... [more]
A0A6H5KAT5_9PHAE4.730e-1868.75RGS domain-containing protein n=1 Tax=Ectocarpus s... [more]
D7FTZ7_ECTSI1.450e-1767.19Similar to regulator of G-protein signaling 16 n=1... [more]
D7FU07_ECTSI1.550e-1764.06Signal transducer, putative n=1 Tax=Ectocarpus sil... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
F-serratus_M_contig681contigF-serratus_M_contig681:189103..192810 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Fucus serratus MALE vs UniRef902022-09-19
OGS1.0 of Fucus serratus male2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score89.0
Seed ortholog evalue9e-16
Seed eggNOG ortholog2880.D7FU02
Preferred nameRGS1
KEGG koko:K16449
Hectar predicted targeting categoryother localisation
GOsGO:0001101,GO:0003674,GO:0004888,GO:0004930,GO:0005096,GO:0005575,GO:0005622,GO:0005623,GO:0005634,GO:0005737,GO:0005768,GO:0005886,GO:0006950,GO:0007154,GO:0007165,GO:0007186,GO:0008047,GO:0008150,GO:0009414,GO:0009415,GO:0009628,GO:0009719,GO:0009725,GO:0009737,GO:0009743,GO:0009746,GO:0009749,GO:0009756,GO:0009987,GO:0010008,GO:0010033,GO:0010035,GO:0010182,GO:0012505,GO:0016020,GO:0023052,GO:0030234,GO:0030695,GO:0031090,GO:0031410,GO:0031982,GO:0033993,GO:0034284,GO:0038023,GO:0042127,GO:0042221,GO:0043085,GO:0043087,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0043547,GO:0044093,GO:0044422,GO:0044424,GO:0044433,GO:0044440,GO:0044444,GO:0044446,GO:0044464,GO:0050789,GO:0050790,GO:0050794,GO:0050896,GO:0051336,GO:0051345,GO:0051716,GO:0060089,GO:0060589,GO:0065007,GO:0065009,GO:0070887,GO:0071310,GO:0071322,GO:0071944,GO:0097305,GO:0097708,GO:0098588,GO:0098772,GO:0098805,GO:1901700,GO:1901701
EggNOG free text desc.Regulator of G protein signaling domain
EggNOG OGs2CNEE@1,2QVMV@2759
Ec32 ortholog descriptionhypothetical protein
Ec32 orthologEc-00_003150.1
COG Functional cat.S
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000
Exons2
Model size197
Cds size195
Stop0
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622932086.566925-CDS-F-serratus_M_contig681:189102..1892131622932086.566925-CDS-F-serratus_M_contig681:189102..189213Fucus serratus maleCDSF-serratus_M_contig681 189103..189213 -
1690964140.407962-CDS-F-serratus_M_contig681:189102..1892131690964140.407962-CDS-F-serratus_M_contig681:189102..189213Fucus serratus maleCDSF-serratus_M_contig681 189103..189213 -
1622932086.6133754-CDS-F-serratus_M_contig681:192726..1928101622932086.6133754-CDS-F-serratus_M_contig681:192726..192810Fucus serratus maleCDSF-serratus_M_contig681 192727..192810 -
1690964140.4239163-CDS-F-serratus_M_contig681:192726..1928101690964140.4239163-CDS-F-serratus_M_contig681:192726..192810Fucus serratus maleCDSF-serratus_M_contig681 192727..192810 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_F-serratus_M_contig681.17869.1prot_F-serratus_M_contig681.17869.1Fucus serratus malepolypeptideF-serratus_M_contig681 189103..192810 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_F-serratus_M_contig681.17869.1

>prot_F-serratus_M_contig681.17869.1 ID=prot_F-serratus_M_contig681.17869.1|Name=mRNA_F-serratus_M_contig681.17869.1|organism=Fucus serratus male|type=polypeptide|length=65bp
CRDKILETDVTSFDIFEDARKEVLHVMETNFKREFTETEGFRRIVDATER
EQQELRLLRERGMMP
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mRNA from alignment at F-serratus_M_contig681:189103..192810-

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_F-serratus_M_contig681.17869.1 ID=mRNA_F-serratus_M_contig681.17869.1|Name=mRNA_F-serratus_M_contig681.17869.1|organism=Fucus serratus male|type=mRNA|length=3708bp|location=Sequence derived from alignment at F-serratus_M_contig681:189103..192810- (Fucus serratus male)
TGCAGGGATAAGATCCTGGAGACGGACGTGACCTCGTTTGATATTTTCGA
GGATGCCCGGAAAGAAGTCCTTCACGTCATGGAGGTGTGTGTGTGTGTGT
GTGTGTGTGTGTATGTGTGTGTGTATGTGTGTGTGTGTGTGTGTGTGCGT
GTTTGTATGTATGTGTGTGTATTTACTGAACTATACTTGATCGTTCTTTC
CGGCCCTGTCATGGTTGTCATTGTATGCCACTCGCATTGGTCCTCCAAAA
GGGGGACCAATGGTACTGTATGTTGTGTGTGTAGTTCTCAAATTGCACAT
AACCACGCAAAAAGACCGTTTCGTCCTAGTCATCCTATGCCACCCGTATG
TGTGTGTATATATATTTAATTGAACATAACCGCGCGGTCTAGCCCTACCA
TTCTGGTTTTCATATATCACTCTCATTGTTGTGCGTGTGTCTAATATATG
CATGTTTGTGTGTGTGTTCGTATATTTATTAAGTTGCACATAACCACGCA
ATCCGGCCCTGTCACCCTACTTGTTATCATTCTGTGTGTATGTGGGCATG
CCTGTGCGTGTGTATGTATGTGTGTGTTTATTAGTTTCAAATAAACGCGC
AGTCGGGCCCTGTCACCCTCCTAGTGGTTCTATGATGCTACTCTGATTGG
CCTCCCCGAGGGGGGATGGTGTGTATATGCGTGCGTGCGTGTCTGTGTGT
GTTTATAAAATTGCACATAGCCGCGCAATCCGGCTTTATCATCCTAGACT
GGTCATTCTGTCTGTGTGTGTTTGATTGCATGCGTTTTTCTTTAATCGTG
TGTATGCTAGAAATGAGCACCTGGCTGACTCCTAACCGTTCTATGAAAGG
GTGTTTGCGGTGGGTGCCTTGCATGCCTGCCTTGTCTGACAGGAGAGGAG
GCGGGTGTCGTCACTCTACGTCAAGTGTAGAGATTAGTTAGAATGTTCTA
TTTTGGCGCGGTCACACATTGCATCATTAGTTCTCAGCCGTAACGATTCC
ATGCACCGCATACAGCTCTCGGGTGGCACGACTATACGACAAGGCTGTAG
ATGATGACCATAAATACATACATACATGCATGCATACATACATACATACA
TACATACATACATACATACATACACACATCCATACATCCATCCATACATG
GCTACATACATACATACTATATACATACATACATGCGAGTGGCATAGAAT
GACTAGGATGACAGGGCCGGATTGCCCGAGTGGTCTATTAATAGTAGGTA
ACCTGCACACCTGCTAGTATAGTGCGGAAGTCGTGAGTTCGAATCTTACC
AAACAAAGTAGGATTTGTATTCATTCTTTTGTAACAGAAAGCGTGGGAAC
GTGAGTAAGTCAAATTCAACCTTTGGTTGACGAGTTGTAAGATTGTTGAA
CTTGCATCGCGATAAAATATTGTAAGGCGTGAACCGGGGAGAGATGGGAA
GATCCTTTGTGACCACAGGCAGGATAGTATTTGTGAGGCCCATCGGCTAT
AAGTGAGATTAAAAAAAATAAAAAAATCGCGGTTATGTGCAATTTAATAA
ATATACATACATATATACATAAATGCATACAGTCATACATACATACATAA
TCGCCGTTACTTTCAACATGCCCACTTCATACCAGTAGTGGATGTGGGAA
AGAGAGGCGTATATTAATTGGTCCATAGTGACCTGCTAAGGCAGCACTCG
GTCGGAACTACTGGCCCTGTACCGACGGACTCTCGGCAGTGAACGCCATC
GGTACGCAGTTGCGTGACCCGATAAACTCGGAACTTACCCGATGGCGTAT
GGCGGTTTAATATATATATATTATATATATATATATATATATGCCGCCGC
GGAACTCGGGAGGAATCCCGTAAGTAAGCACCAGATCCACCCTGAGTATG
GAGATGAGCAGGCTGACGCGGGACGGGACTGCCGAAACCGTCTCGCGAGA
CCAAATTTTCAGGCACGAACGCGGACAGGGGAATATTCTTTTTCCTGTTC
AGCTGACCACGAGCAGGATTGGCAACCTTACTAGGTTGATCCATACTCTT
GCTATATGTGTGACCATACATACATACATACAAACATACATCCATGCATA
CATATATACATACATACATACATACATACATAAATACGGACATACATATA
TACCTACCTACCTACATATATACATACATACATCGCGTCTGATCAGAGGT
TTTCGTAATAAGTTTCATTGATTCCCCCTTGGGAGGATCAATACGAGTGG
CATAGAATGACTAGGATGACAGGGCCGGATTGCGCGGTTATGTGCAATTT
AATAAATATACAGACACACATACATACATACTTACATACATACATACATA
CATAAATACGGACATACATACCTACCTACCTACATACATATATACATACA
TGCATATACGTTGACGAGGAGTAATCCCTTGGTGGGTTACATGCAATAGC
ATAGAATGGGTAGATTACAGGGCCGGATTGCGCGGTTATATGCAATTTAA
TAAATATACATACACATACATACATACATACATAAGTTTCATTGAGGGTA
TGGCTATTTGGCCCACCGGTGCGCTATTCTCTTGTTCCCCCCCCCACCTG
GATCTCGCGTCAGAAGACTTGGTTGGTCACAATACATGCATGGGTGTGTT
AGGGTTAGGGTTAGCTATTATCGGGTCTATCGTGCAGCCCAAACGATTCC
GTCTACGTGCTACCACGGCCGATGGGGTGGTTGGTGTTTTCTGGTATGTA
TGTCTATTTGCCCTTACATACTAGAAAATACCACCACCCCACCGGCCGTA
ACATCACGTAGACAGGACTGTTTGGGCTGCCCGAATGACCCCATGATAGC
CCTGCAACGCGCCAACACACGCGTGCATGTATTGTGACCAACCATGTCTT
CTGACGCGAGATCCAGGTGGTGGGGTAAATTTGGGTATGCCCCACCGGTG
GGGCAAATAGCCATTCCCTTTCATTGATTTCCCCTTGGGAGGATCAATAC
GAGTGTCATAGAATGACTAGGATGACAGGGCCGGATTGTGCGGTTATGTG
CAATTTAATAAATACACACACACACACACATACCTACCTACATAAATATA
TTCATACATACATATATACATATATACGCACACCCCCCCGACTACGGCTT
TTACATGGCCTATTTATTGTGGTTGGAGGACCGTAATGCAGCGCTCGCCG
CCCGAGTGGTCTAGTAACTAACCTGCACACGTGCTAGTACGGAGGTCGTG
ATTTCGATTCTTGTCAAAGCAGGATTTGTGTTCTTTCTTCCGTAAAGGGA
AGCGTGGGAACTTGAGTAGGCAAAATTCAACCATGTTGGTTGACGAGTTG
TAATATCGCCGAACTTGGCATCGCGATAAAAAATCGCAAGGCGTGAACCG
GTAAGAGAAGGGAAGATCCTTAGTGATGGCGACCACATGGCAGATTGGTA
ATGATGGGGATCATCGGCTATACTATTAATAAGTGAGATTACAAAATAAT
AATATAATGCGGCGCCTCCGATACAGTTTACTATCACTAGAGCCGAGCAA
GCTCTACCCCCGCTTTCCATTCTATCCGTTCTCTACGCGCGTCGAAGACA
AATTTCAAGAGGGAGTTCACAGAAACGGAAGGCTTCCGCCGCATCGTGGA
CGCAACCGAACGAGAACAACAAGAGCTGAGGCTCCTGCGTGAGAGAGGTA
TGATGCCC
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Coding sequence (CDS) from alignment at F-serratus_M_contig681:189103..192810-

>mRNA_F-serratus_M_contig681.17869.1 ID=mRNA_F-serratus_M_contig681.17869.1|Name=mRNA_F-serratus_M_contig681.17869.1|organism=Fucus serratus male|type=CDS|length=390bp|location=Sequence derived from alignment at F-serratus_M_contig681:189103..192810- (Fucus serratus male)
TGCAGGGATAAGATCCTGGAGACGGACGTGACCTCGTTTGATATTTTCGA
GGATGCCCGGAAAGAAGTCCTTCACGTCATGGAGTGCAGGGATAAGATCC
TGGAGACGGACGTGACCTCGTTTGATATTTTCGAGGATGCCCGGAAAGAA
GTCCTTCACGTCATGGAGACAAATTTCAAGAGGGAGTTCACAGAAACGGA
AGGCTTCCGCCGCATCGTGGACGCAACCGAACGAGAACAACAAGAGCTGA
GGCTCCTGCGTGAGAGAGGTATGATGCCCACAAATTTCAAGAGGGAGTTC
ACAGAAACGGAAGGCTTCCGCCGCATCGTGGACGCAACCGAACGAGAACA
ACAAGAGCTGAGGCTCCTGCGTGAGAGAGGTATGATGCCC
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