mRNA_F-serratus_M_contig1098.888.1 (mRNA) Fucus serratus male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_F-serratus_M_contig1098.888.1
Unique NamemRNA_F-serratus_M_contig1098.888.1
TypemRNA
OrganismFucus serratus male (Fucus serratus male (Toothed wrack or serrated wrack))
Homology
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BLAST of mRNA_F-serratus_M_contig1098.888.1 vs. uniprot
Match: D7FZG1_ECTSI (Uncharacterized protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FZG1_ECTSI)

HSP 1 Score: 107 bits (268), Expect = 2.870e-29
Identity = 54/71 (76.06%), Postives = 59/71 (83.10%), Query Frame = 1 
Query:    1 MRQATTFWRLAGMTYLEYASKATTVVRGALKEPARTQALARDVVSYNRAVFENGKPLPKSPITSLKEASAT 213
            M QATTFWRLAGMTYLEYASKATTV+RGALKEPAR+ ALAR+ V YNR VF  G+P  K P+ SLKEA AT
Sbjct:    1 MSQATTFWRLAGMTYLEYASKATTVMRGALKEPARSTALAREAVGYNRNVFVGGQPQGKLPVASLKEAGAT 71
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BLAST of mRNA_F-serratus_M_contig1098.888.1 vs. uniprot
Match: A0A835YQS1_9STRA (Uncharacterized protein n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835YQS1_9STRA)

HSP 1 Score: 80.1 bits (196), Expect = 2.720e-18
Identity = 37/67 (55.22%), Postives = 51/67 (76.12%), Query Frame = 1 
Query:    4 RQATTFWRLAGMTYLEYASKATTVVRGALKEPARTQALARDVVSYNRAVFENGKPLPKSPITSLKEA 204
            + ATTFWR+AG++YLEYA+  TT +R  LKEP R++A+ R+ VSYNRAVF++G    K P+ SL +A
Sbjct:    4 KAATTFWRIAGLSYLEYAAATTTALRMGLKEPMRSKAMTRNAVSYNRAVFKDGVSGAKKPVKSLADA 70
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BLAST of mRNA_F-serratus_M_contig1098.888.1 vs. uniprot
Match: A0A1Z5K8B7_FISSO (Uncharacterized protein n=2 Tax=Fistulifera solaris TaxID=1519565 RepID=A0A1Z5K8B7_FISSO)

HSP 1 Score: 75.9 bits (185), Expect = 1.260e-16
Identity = 33/64 (51.56%), Postives = 49/64 (76.56%), Query Frame = 1 
Query:   13 TTFWRLAGMTYLEYASKATTVVRGALKEPARTQALARDVVSYNRAVFENGKPLPKSPITSLKEA 204
            TTFWR+AGM+YL+Y +KA   +R  LKEPA+T+A+A++   YN AV+E GK  PK P+ ++++A
Sbjct:    7 TTFWRVAGMSYLQYINKAAGTMRSVLKEPAKTRAMAQEAYGYNAAVWEAGKQGPKLPVANIQQA 70
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BLAST of mRNA_F-serratus_M_contig1098.888.1 vs. uniprot
Match: B7G8W9_PHATC (Predicted protein n=4 Tax=Phaeodactylum tricornutum TaxID=2850 RepID=B7G8W9_PHATC)

HSP 1 Score: 73.9 bits (180), Expect = 3.200e-15
Identity = 32/67 (47.76%), Postives = 50/67 (74.63%), Query Frame = 1 
Query:   13 TTFWRLAGMTYLEYASKATTVVRGALKEPARTQALARDVVSYNRAVFENGKPLPKSPITSLKEASAT 213
            TTFWR+AGM+YL+Y +KA T +RGALKEPA+ +A+ ++  +Y  + +++GK  PK P+  L ++ AT
Sbjct:   63 TTFWRMAGMSYLQYVNKAATSMRGALKEPAQRKAMEQEAFAYKVSTWQDGKQSPKVPVDVLAQSGAT 129
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BLAST of mRNA_F-serratus_M_contig1098.888.1 vs. uniprot
Match: A0A7S3L8J2_9STRA (Hypothetical protein n=1 Tax=Amphora coffeiformis TaxID=265554 RepID=A0A7S3L8J2_9STRA)

HSP 1 Score: 65.1 bits (157), Expect = 5.910e-12
Identity = 26/64 (40.62%), Postives = 45/64 (70.31%), Query Frame = 1 
Query:    4 RQATTFWRLAGMTYLEYASKATTVVRGALKEPARTQALARDVVSYNRAVFENGKPLPKSPITSL 195
            +  TTFWR+AG++YL+Y +KA + +RGALKEPA+++   +D  S+ ++ +  G   PK+ +T +
Sbjct:   47 KPVTTFWRMAGLSYLQYVNKAASTIRGALKEPAKSRLAVQDTFSFKKSSWSGGVQGPKTEVTRM 110
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BLAST of mRNA_F-serratus_M_contig1098.888.1 vs. uniprot
Match: A0A0J8CI46_BETVV (Uncharacterized protein n=1 Tax=Beta vulgaris subsp. vulgaris TaxID=3555 RepID=A0A0J8CI46_BETVV)

HSP 1 Score: 62.0 bits (149), Expect = 3.600e-11
Identity = 31/67 (46.27%), Postives = 43/67 (64.18%), Query Frame = 1 
Query:   10 ATTFWRLAGMTYLEYASKATTVVRGALKEPARTQALARDVVSYNRAVFENGKPLPKSPITSLKEASA 210
            A  FWR AGMTY+ Y++    +VR  LKEP +++ALAR+ V Y+ + FENGKP  + P     +A A
Sbjct:    6 AVPFWRAAGMTYISYSNICANLVRNCLKEPFKSEALAREKVHYSISKFENGKP--QKPTIRSDDAQA 70
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BLAST of mRNA_F-serratus_M_contig1098.888.1 vs. uniprot
Match: A0A448YV42_9STRA (Uncharacterized protein n=1 Tax=Pseudo-nitzschia multistriata TaxID=183589 RepID=A0A448YV42_9STRA)

HSP 1 Score: 62.0 bits (149), Expect = 3.690e-11
Identity = 28/65 (43.08%), Postives = 44/65 (67.69%), Query Frame = 1 
Query:   10 ATTFWRLAGMTYLEYASKATTVVRGALKEPARTQALARDVVSYNRAVFENGKPLPKSPITSLKEA 204
            +TT+WR+AGMTYL+Y ++A   VR  LKEP +++ LA+    YN + +E+G    K  I++L +A
Sbjct:    5 STTYWRIAGMTYLQYVNRAAGAVRSGLKEPTKSKLLAQSEFKYNASAWESGIQGAKKEISALGQA 69
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BLAST of mRNA_F-serratus_M_contig1098.888.1 vs. uniprot
Match: A0A1E7ER17_9STRA (Uncharacterized protein n=1 Tax=Fragilariopsis cylindrus CCMP1102 TaxID=635003 RepID=A0A1E7ER17_9STRA)

HSP 1 Score: 62.0 bits (149), Expect = 3.690e-11
Identity = 29/65 (44.62%), Postives = 44/65 (67.69%), Query Frame = 1 
Query:   10 ATTFWRLAGMTYLEYASKATTVVRGALKEPARTQALARDVVSYNRAVFENGKPLPKSPITSLKEA 204
            AT FWR+AGM+YL+Y ++A   VR ALKEP +++  A+   SY  + + +G  + K+ ITSL +A
Sbjct:    5 ATNFWRIAGMSYLQYVNRAAGSVRSALKEPMKSKLSAQGEFSYKASAWVDGTQMSKTEITSLSQA 69
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BLAST of mRNA_F-serratus_M_contig1098.888.1 vs. uniprot
Match: W7U8A3_9STRA (Atp synthase subunit epsilon n=2 Tax=Monodopsidaceae TaxID=425072 RepID=W7U8A3_9STRA)

HSP 1 Score: 61.6 bits (148), Expect = 5.370e-11
Identity = 32/68 (47.06%), Postives = 47/68 (69.12%), Query Frame = 1 
Query:   10 ATTFWRLAGMTYLEYASKATTVVRGALKEPARTQALARDVVSYNRAVFEN---GKPLP--KSPITSLK 198
            ATT+WR+AG+TYL+Y SKA  V+R ALKEP RT+ +A++ + + R  +     G+P+   K  +TSLK
Sbjct:    5 ATTYWRIAGLTYLQYTSKAAGVLRAALKEPLRTKTMAKEKIFFKRLEWSPKMIGQPVGPLKVEVTSLK 72
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BLAST of mRNA_F-serratus_M_contig1098.888.1 vs. uniprot
Match: A0A0K9RSM6_SPIOL (Uncharacterized protein n=1 Tax=Spinacia oleracea TaxID=3562 RepID=A0A0K9RSM6_SPIOL)

HSP 1 Score: 60.8 bits (146), Expect = 1.010e-10
Identity = 28/54 (51.85%), Postives = 38/54 (70.37%), Query Frame = 1 
Query:   10 ATTFWRLAGMTYLEYASKATTVVRGALKEPARTQALARDVVSYNRAVFENGKPL 171
            A  FWR AGMTY+ Y++    +VR  LKEP +++A+AR+ V Y+ A FENGK L
Sbjct:    6 AVPFWRAAGMTYISYSNICANLVRNCLKEPFKSEAIAREKVHYSLAKFENGKSL 59
The following BLAST results are available for this feature:
BLAST of mRNA_F-serratus_M_contig1098.888.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Fucus serratus MALE vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
D7FZG1_ECTSI2.870e-2976.06Uncharacterized protein n=1 Tax=Ectocarpus silicul... [more]
A0A835YQS1_9STRA2.720e-1855.22Uncharacterized protein n=1 Tax=Tribonema minus Ta... [more]
A0A1Z5K8B7_FISSO1.260e-1651.56Uncharacterized protein n=2 Tax=Fistulifera solari... [more]
B7G8W9_PHATC3.200e-1547.76Predicted protein n=4 Tax=Phaeodactylum tricornutu... [more]
A0A7S3L8J2_9STRA5.910e-1240.63Hypothetical protein n=1 Tax=Amphora coffeiformis ... [more]
A0A0J8CI46_BETVV3.600e-1146.27Uncharacterized protein n=1 Tax=Beta vulgaris subs... [more]
A0A448YV42_9STRA3.690e-1143.08Uncharacterized protein n=1 Tax=Pseudo-nitzschia m... [more]
A0A1E7ER17_9STRA3.690e-1144.62Uncharacterized protein n=1 Tax=Fragilariopsis cyl... [more]
W7U8A3_9STRA5.370e-1147.06Atp synthase subunit epsilon n=2 Tax=Monodopsidace... [more]
A0A0K9RSM6_SPIOL1.010e-1051.85Uncharacterized protein n=1 Tax=Spinacia oleracea ... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
F-serratus_M_contig1098contigF-serratus_M_contig1098:13724..17380 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Fucus serratus MALE vs UniRef902022-09-19
OGS1.0 of Fucus serratus male2021-02-24
Properties
Property NameValue
Taxonomic scopeViridiplantae
Seed ortholog score62.8
Seed ortholog evalue7.8e-08
Seed eggNOG ortholog161934.XP_010677215.1
KEGG koko:K02135
KEGG TC3.A.2.1
KEGG Pathwayko00190,ko01100,ko04714,ko05010,ko05012,ko05016,map00190,map01100,map04714,map05010,map05012,map05016
KEGG ModuleM00158
Hectar predicted targeting categoryother localisation
GOsGO:0005575,GO:0005622,GO:0005623,GO:0005737,GO:0005739,GO:0005740,GO:0005743,GO:0005753,GO:0016020,GO:0016469,GO:0019866,GO:0031090,GO:0031966,GO:0031967,GO:0031975,GO:0032991,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0044422,GO:0044424,GO:0044425,GO:0044429,GO:0044444,GO:0044446,GO:0044455,GO:0044464,GO:0045259,GO:0098796,GO:0098798,GO:0098800
EggNOG free text desc.ATP synthase subunit epsilon
EggNOG OGs37W9H@33090,3GK5A@35493,KOG3495@1,KOG3495@2759
COG Functional cat.C
Best tax levelStreptophyta
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko00002
Exons3
Model size219
Cds size219
Stop1
Start1
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_F-serratus_M_contig1098.888.1prot_F-serratus_M_contig1098.888.1Fucus serratus malepolypeptideF-serratus_M_contig1098 13724..17380 +


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622928956.7121048-CDS-F-serratus_M_contig1098:13723..137741622928956.7121048-CDS-F-serratus_M_contig1098:13723..13774Fucus serratus maleCDSF-serratus_M_contig1098 13724..13774 +
1690962645.2448702-CDS-F-serratus_M_contig1098:13723..137741690962645.2448702-CDS-F-serratus_M_contig1098:13723..13774Fucus serratus maleCDSF-serratus_M_contig1098 13724..13774 +
1622928956.7265134-CDS-F-serratus_M_contig1098:16548..166751622928956.7265134-CDS-F-serratus_M_contig1098:16548..16675Fucus serratus maleCDSF-serratus_M_contig1098 16549..16675 +
1690962645.2577553-CDS-F-serratus_M_contig1098:16548..166751690962645.2577553-CDS-F-serratus_M_contig1098:16548..16675Fucus serratus maleCDSF-serratus_M_contig1098 16549..16675 +
1622928956.753097-CDS-F-serratus_M_contig1098:17339..173801622928956.753097-CDS-F-serratus_M_contig1098:17339..17380Fucus serratus maleCDSF-serratus_M_contig1098 17340..17380 +
1690962645.271005-CDS-F-serratus_M_contig1098:17339..173801690962645.271005-CDS-F-serratus_M_contig1098:17339..17380Fucus serratus maleCDSF-serratus_M_contig1098 17340..17380 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_F-serratus_M_contig1098.888.1

>prot_F-serratus_M_contig1098.888.1 ID=prot_F-serratus_M_contig1098.888.1|Name=mRNA_F-serratus_M_contig1098.888.1|organism=Fucus serratus male|type=polypeptide|length=73bp
MRQATTFWRLAGMTYLEYASKATTVVRGALKEPARTQALARDVVSYNRAV
FENGKPLPKSPITSLKEASATG*
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mRNA from alignment at F-serratus_M_contig1098:13724..17380+

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_F-serratus_M_contig1098.888.1 ID=mRNA_F-serratus_M_contig1098.888.1|Name=mRNA_F-serratus_M_contig1098.888.1|organism=Fucus serratus male|type=mRNA|length=3657bp|location=Sequence derived from alignment at F-serratus_M_contig1098:13724..17380+ (Fucus serratus male)
ATGCGACAGGCAACGACATTCTGGCGGCTGGCGGGAATGACCTACCTAGA
GGTGGGATATATAATTTTGTGTCTCTGGTTATCATCATCCGGCCCTGCTC
CGAAAAGTAAGAGGAGGAGCGAGTTACTGCTTCGCCCTCCATACCTGGAG
AAACAAAGTCCTTGGAAGTTTGTTTAGTTCTAATCATTTTAGCGAGAAGG
CCACAGTCTTGGGAATGGACCAGTCTGAACGAGTGCTAGCCGCAAGAGTT
GGGTGTGTTCTGACTCTCTTGTCAGTTGTTGCATCACCATCCTTCTCAGC
CGAGGAAAAGGAACTTTGCGAGAGTCCCGAATAAGCTCACAGAACAGGCA
CAGAATCCACTAGCTGTCTGGAGAGACACAAAACTACGTAATAAGGGTAC
AGGGGTGCGAACTACTATCGTCTGTAAACTGTTATTATCGATCGTTCTAC
TGCAAGGCGTGTCGGGGCTACAACCCCATCTTCTCGCTGTGTTTCTTTGT
CTTGTCTGGGCTCTTTTGTCCTTTACGGGTAACATGTTCAGAACGCCCCG
GAATGGCCAGTGCCTACGTACATGTTGCATTTTGTGATCATCGCTCTGTG
GGGCTTCTGTTTTGTCATCGAATCTTATGCGCACAATATCGACCAGATTT
AGCCTGAGTCTGTGCTGTAGAATGAGCAGGCTGACGCGTGACTGGACGGC
CGAACCCGTCTCGCGAGACCAAGTTCTCAGGCGCGAGCGGGGACACGGAA
ATATTTAGTTTCCATGCTTAGCTGACCACGAGTAGGATTGGTAACCTTAC
CCGGTTGATCCATACTCAGCTATTATTATACATGATGACAAATATATACC
GCTGTAATACGACGACAGTACTGTTTTCCCCTTATATTGTACTAATAGTA
ATATGCTTTACGTACTATTATTAGTATCCCCAAAGGAGTAAAGCGCCCTA
TCGCCATAGAATACTTCACACCATTAGTGGGTGTGGGAAAAAGAGGCGCA
CATTAATTGGTCCATGGTGACCTGCAAAATGGCAGCACCCGTTACTGGAA
CTACACTGAGGACTACTGGCCCTGTGACGGTGGAGTCTCGGCAGTGAGCA
CCATCGGTACGCAATTGTGTGACCCGATAAACTCGGGGCTGACCCGATGG
CGTGTGGCGGTTTAAGAAAATTAATGGACCCCGCCGCGGAAATCGGGAGG
AATCCCGCAAGTAAGCATCATATTCCACCTTGAGTATGGAGAAAGAGTGG
GCTGACGTGGAACGGGACGGTTGAACCCGTCTCGCGAGACCAAAATCTCA
GGCGCGTACAGGCACAGAGGAATATTCGTTTTCCCCTGTTCAGCTGACCA
CGAGCAGAATTGGCAACTTTACCCGGTTGATCCATACTCTTACTATATGT
GATGACTATACATACATACATATATACTTAACTACTACTGTACTGTAGAC
TACTGCAGTACGTGTAGATAAGTGTAACTGTAAGCTTCCCAGTTGAAAGT
GTTGCGGCTATAGTATACCCGTTTAATACCCCCATAGTCTATAACAGAGC
GCTTCCAAACTCCCGAGGACATGAATATCGTATTGCATTGTATTCGCTTG
CCATAATAGGCAGCACCTACTTCTCTGGGGGCACTTTTTGTGGGAGAATC
CGTCGGCTAAAGATGGGTTTGGAGAGAAGGGCGTAACCCAACCACATACT
GTGGTTTGAAGAAGAAAAAAACTGAACGCATTTAGACCTCCTGGGCATCC
CCCAGTCAAGAGGGGGAAAATGTCAGGAGGTTTACTGTACAGTGTAGGTG
GAATTCCAGCCCACACGAAGTCACGAGTAGGCCGTTGTACGAGTCTACTT
CATACCAATAGTGGGTGTGGGAAAGCGAGGCGAATATTAATTATTAATTG
GTACATGGTGACCTGCCAAGGCAGCATTCGTTCGGAACTACCTTGAGGTT
TACTGGCCCTATGCCGGCGGACTCTCGGTAGTGAACGCCATCGGTACGCA
ATTGTGTGACCCGATAAACTCGGGACTTACCCGATGGCGTATGGCGGTTT
TATATAAATATATGGACGCCGCCGCGGAACTCGGGAGGAATCCCGTAAGT
AAGTACCAGATTCAGCCTTGTAGATGAGCAGGCTGACGCGGGACGGGACT
GCTGAACCCGTCTCGCGAGACCAAATTCTCAGGCACGCACGGGGACAGGG
AAATATTCATTTTCCCTGTTCAGCTGACCACGAGCAGAATTGGCAACCTT
ACACGGTTGATCCATACCCTGCTATATGTGATGGCCATACATACATACAT
ATATGTACCTAAACGATTTCACGAGTAGGCCGTTGTACGAGTGTATGTAC
CTAAACGGGAGGATTGTGCACGGGAGGGGACCACGTACATGTCGTACTCG
GTATTAAATATGATACCTGCATGGAAATCTGCAAAACAAATATTTTAGGC
GCGGAAATCCACCAGAACCCCGCCCCTGCGTAACGCGATCTTATCGTGTT
TTTTTTGTAGGCGCGCCCACACGAGAGGCACGCTGCCCTTAAGCGAGACT
CTTTCATGTTTCGCCAGGCGACGCCTATCCAGGTCAACGAAACCCCTGCC
GTAAAACGCAACTGCTCGTGGCCCAACTGAGCGTACCATAAAACACAAGA
ACAGGAGTAAAACAACAAAGCCAGAACTATGTACAGCTGCTTTGGTTTTC
GTTTTGTTCTTAAATCCGGTTCGGTTTGGTTCGGTTCGGTAAGACCCTCT
TTGCCGCATTTCTAATTATTTTCGTGCTCTTCTCGCTCCTCGCCTCGTCC
TACCTTCGCGACGCTCTCGTGGCAGTACGCCAGCAAGGCGACCACGGTGG
TGCGCGGGGCGCTCAAGGAGCCGGCGCGCACCCAAGCGCTCGCGAGAGAT
GTGGTCAGCTACAACCGCGCCGTGTTCGAGAACGGCAAGCCGCTTCCCAA
ATGTGAGCGCCGGTTCTGTACCAGCAAGATGCTCAGAAGATTAAGATGCG
TTCATATTTTTCTTTATACAGGATCGCGAGTGTGGAGAAAAGGGGAGGGG
GAGATTCTTGCCCCCTTTTTGCGCACTCGTCGAAGTACTACAAAAACCTA
TATTGGTCCCGACGTGTGGACATCGAAAAGGCGACGCCCAGGATCCGACC
GAGATTTTATTTGCTTACGTGTGGTTTATGAATGTTTCTGCTGTGGTGTA
AAGCATTCCTGTTGCAGGTTACGAAAATATACTTTGGGATACTTTTGATA
GCAGGGCGAGACACGTCGAAATACATTAAATTGGGCCCGTGTTCGGCTCG
CTCACGCCGCGTATCCACACGAAGTTTACAATCTAACCACTGGTCTGACT
TAGGACATTCCATCCCCCCGCCCCCCTCCCCAAAAAAAATAGCCTTGTGA
GAACGGATCATCCGTACAGCACGTAATGTAAATGTGGGTATCTGTAGCGC
TCTCGAGTACGACGTCCCGTGTACCAGCTGCGCTCGGCAGCATTACAACC
TCGCGCACATATTTGTCAACTTAGTTGAATCTATCTGTAAGAGTTTTATC
TCAGTATCGTGTACCTCTTTTGCCTTGCTCGCTCGTGCGGCACCTCTTCT
TCTACTCTGCACACAGCCCCGATCACGTCTCTCAAGGAGGCAAGCGCAAC
GGGTTAA
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Coding sequence (CDS) from alignment at F-serratus_M_contig1098:13724..17380+

>mRNA_F-serratus_M_contig1098.888.1 ID=mRNA_F-serratus_M_contig1098.888.1|Name=mRNA_F-serratus_M_contig1098.888.1|organism=Fucus serratus male|type=CDS|length=438bp|location=Sequence derived from alignment at F-serratus_M_contig1098:13724..17380+ (Fucus serratus male)
ATGCGACAGGCAACGACATTCTGGCGGCTGGCGGGAATGACCTACCTAGA
GATGCGACAGGCAACGACATTCTGGCGGCTGGCGGGAATGACCTACCTAG
AGTACGCCAGCAAGGCGACCACGGTGGTGCGCGGGGCGCTCAAGGAGCCG
GCGCGCACCCAAGCGCTCGCGAGAGATGTGGTCAGCTACAACCGCGCCGT
GTTCGAGAACGGCAAGCCGCTTCCCAAATTACGCCAGCAAGGCGACCACG
GTGGTGCGCGGGGCGCTCAAGGAGCCGGCGCGCACCCAAGCGCTCGCGAG
AGATGTGGTCAGCTACAACCGCGCCGTGTTCGAGAACGGCAAGCCGCTTC
CCAAATCCCCGATCACGTCTCTCAAGGAGGCAAGCGCAACGGGTTAACCC
CGATCACGTCTCTCAAGGAGGCAAGCGCAACGGGTTAA
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