EsuBft3723_3 (polypeptide) Ectocarpus subulatus male Bft15b

You are viewing a polypeptide, more information available on the corresponding mRNA page

Overview
NameEsuBft3723_3a-0001
Unique NameEsuBft3723_3
Typepolypeptide
OrganismEctocarpus subulatus male Bft15b (Ectocarpus subulatus male Bft15b)
Sequence length519
Homology
BLAST of EsuBft3723_3a-0001 vs. uniprot
Match: A0A6H5KDF8_9PHAE (Subtilisin n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5KDF8_9PHAE)

HSP 1 Score: 1047 bits (2708), Expect = 0.000e+0
Identity = 519/519 (100.00%), Postives = 519/519 (100.00%), Query Frame = 0
Query:    1 MSQVNSKPADYPVQFSTPYKCNGCNVVTYRAVHPAVKTPDQGSTSSGQDSFTYFSDYFGDPYDYDDSEYISHDDYSGEYFSDTVVPALKTLILSQLPAATDSRSTARVLTDNLFVEKASGLTDAEVRQAVKTWLSVEDDPEVLEEEIAMELQAVDEQLAGVTLDDDVPSEEEDDDGGLDDLAYHLSYLYDDTSYASSSTSPSFTGGFHKDTIGGHGTSTAGSAAGAISSQSSLEEEACYGDELPACAGGCISASDVEAMLDDSTFDLDVFCPMYTCDGNTDIPSSECLSDDPVENLHQHGGAAPGAQISVFDVSYVGTEVYASWAGNDLWYSAMETGAKIHTNSWGGATLCQLTEKEYMYDMFMYENPEHLLIFSAGNDGGYEDIPNREACTVGSPALGKNSLAVGATSSGPFPGTRTGDDGNLFYEEVGLTDYSPEGYPWICLMPYLGTPSASTEPAGVDTVAYFSSQGPTLDGRIKPDVVAPGDQVASASSDGTDEHSCKLAGPPLPAPWLPVPLPW 519
            MSQVNSKPADYPVQFSTPYKCNGCNVVTYRAVHPAVKTPDQGSTSSGQDSFTYFSDYFGDPYDYDDSEYISHDDYSGEYFSDTVVPALKTLILSQLPAATDSRSTARVLTDNLFVEKASGLTDAEVRQAVKTWLSVEDDPEVLEEEIAMELQAVDEQLAGVTLDDDVPSEEEDDDGGLDDLAYHLSYLYDDTSYASSSTSPSFTGGFHKDTIGGHGTSTAGSAAGAISSQSSLEEEACYGDELPACAGGCISASDVEAMLDDSTFDLDVFCPMYTCDGNTDIPSSECLSDDPVENLHQHGGAAPGAQISVFDVSYVGTEVYASWAGNDLWYSAMETGAKIHTNSWGGATLCQLTEKEYMYDMFMYENPEHLLIFSAGNDGGYEDIPNREACTVGSPALGKNSLAVGATSSGPFPGTRTGDDGNLFYEEVGLTDYSPEGYPWICLMPYLGTPSASTEPAGVDTVAYFSSQGPTLDGRIKPDVVAPGDQVASASSDGTDEHSCKLAGPPLPAPWLPVPLPW
Sbjct:    1 MSQVNSKPADYPVQFSTPYKCNGCNVVTYRAVHPAVKTPDQGSTSSGQDSFTYFSDYFGDPYDYDDSEYISHDDYSGEYFSDTVVPALKTLILSQLPAATDSRSTARVLTDNLFVEKASGLTDAEVRQAVKTWLSVEDDPEVLEEEIAMELQAVDEQLAGVTLDDDVPSEEEDDDGGLDDLAYHLSYLYDDTSYASSSTSPSFTGGFHKDTIGGHGTSTAGSAAGAISSQSSLEEEACYGDELPACAGGCISASDVEAMLDDSTFDLDVFCPMYTCDGNTDIPSSECLSDDPVENLHQHGGAAPGAQISVFDVSYVGTEVYASWAGNDLWYSAMETGAKIHTNSWGGATLCQLTEKEYMYDMFMYENPEHLLIFSAGNDGGYEDIPNREACTVGSPALGKNSLAVGATSSGPFPGTRTGDDGNLFYEEVGLTDYSPEGYPWICLMPYLGTPSASTEPAGVDTVAYFSSQGPTLDGRIKPDVVAPGDQVASASSDGTDEHSCKLAGPPLPAPWLPVPLPW 519          
BLAST of EsuBft3723_3a-0001 vs. uniprot
Match: A0A6H5JQ52_9PHAE (Subtilisin n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JQ52_9PHAE)

HSP 1 Score: 695 bits (1793), Expect = 6.860e-244
Identity = 358/484 (73.97%), Postives = 374/484 (77.27%), Query Frame = 0
Query:   37 KTPDQGSTSSGQDSFTYFSDYFGDPYDYDDSEYISHDDYSGEYFSDTVVPALKTLILSQLPAATDSRSTARVLTDNLFVEKASGLTDAEVRQAVKTWLSVEDDPEVLEEEIAMELQAVDEQLAGVTLDDDVPSEEEDDDGGLDDLAYHLSYLYDDTSYASSSTSPSFTGGFHKDTIGGHGTSTAGSAAGAISSQSSLEEEACYGDELPACAGGCISASDVEAMLDDSTFDLDVFCPMYTCDGNTDIPSSECLSDDPVENLHQHGGAAPGAQISVFDVSYVGTEVYASWAGNDLWYSAMETGAKIHTNSWGGATLCQLTEKEYMYDMFMYENPEHLLIFSAGNDGGYEDIPNREACTVGSPALGKNSLAVGATSSGPFPGTRTGDDGNLFYEEVGLTDYSPEGYPWICLMPYLGTPSASTEPAGVDTVAYFSSQGPTLDGRIKPDVVAPGDQV-ASASSDGTDEHSCKLAGPPLPAPWLPVPLPW 519
            KTP QGSTSSGQDSFT+FSD+ GDP+DYDDSEYIS+ DYSGEY+SDT                                                 W                                   SE+    GGLDDL+YHLSYLYDDTSYAS STSPSFTGGFH+DTIGGHGT T+GSAAGAISSQSSL+EEACYGDELP CAGGCISASDV+AMLDDSTF L VFCPMYTCDGN DIPSSECLSDDPVE+LHQHGG APGAQISVFD SYVGTEVYASWAGNDLWYSAMETGAKIHTNSWGGATLCQLTEKEYMYD FMYENPEHLLIFSAGNDGGY+DIPNREACTVGSPALGKN LAVGATSSGPFPGTRTGDDGNLF EEVG+TDYSPEGYPWICLMP LG P ASTEPAGVDTV YFSSQGPTLDG+IKP VVAPG+QV ASASSDGTDEHSCKLAGPP PAPW PVPLPW
Sbjct:  328 KTPHQGSTSSGQDSFTFFSDFIGDPFDYDDSEYISYGDYSGEYYSDT-------------------------------------------------W-----------------------------------SEDSFCAGGLDDLSYHLSYLYDDTSYASPSTSPSFTGGFHQDTIGGHGTGTSGSAAGAISSQSSLKEEACYGDELPGCAGGCISASDVDAMLDDSTFHLVVFCPMYTCDGNIDIPSSECLSDDPVEDLHQHGGVAPGAQISVFDFSYVGTEVYASWAGNDLWYSAMETGAKIHTNSWGGATLCQLTEKEYMYDTFMYENPEHLLIFSAGNDGGYDDIPNREACTVGSPALGKNILAVGATSSGPFPGTRTGDDGNLFCEEVGVTDYSPEGYPWICLMPVLGAPPASTEPAGVDTVGYFSSQGPTLDGQIKPGVVAPGNQVVASASSDGTDEHSCKLAGPPFPAPWSPVPLPW 727          
BLAST of EsuBft3723_3a-0001 vs. uniprot
Match: D8LSW0_ECTSI (Subtilisin n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LSW0_ECTSI)

HSP 1 Score: 685 bits (1768), Expect = 4.960e-236
Identity = 355/489 (72.60%), Postives = 376/489 (76.89%), Query Frame = 0
Query:   15 FSTPYKCNGCNVVTYRAVHPAVKTPDQGSTSSGQDSFTYFSDYFGDPYDYDDSEYISHDDYSGEYFSDTVVPALKTLILSQLPAATDSRSTARVLTDNLFVEKASGLTDAEVRQAVKTWLSVEDDPEVLEEEIAMELQAVDEQLAGVTLDDDVPSEEEDDDGGLDDLAYHLSYLYDDTSYASSSTSPSFTGGFHKDTIGGHGTSTAGSAAGAISSQSSLEEEACYGDELPACAGGCISASDVEAMLDDSTFDLDVFCPMYTCDGNTDIPSSECLSDDPVENLHQHGGAAPGAQISVFDVSYVGTEVYASWAGNDLWYSAMETGAKIHTNSWGGATLCQLTEKEYMYDMFMYENPEHLLIFSAGNDGGYEDIPNREACTVGSPALGKNSLAVGATSSGPFPGTRTGDDGNLFYEEVGLTDYSPEGYPWICLMPYLGTPSASTEPAGVDTVAYFSSQGPTLDGRIKPDVVAPGDQVASASSDGTDEHSCKL 503
            +S P+  +   +V Y  +    KTPDQGSTSSGQDSFTY SDY GDPYDYDDSEYIS+DDYSGEY+SDT                                                 W                   + D   AGV                LDDL+YH SYL+DDTSY+SSSTSPSFTGGFHKDT+GGHGT TAGSAAGAIS+QS LEEEACYGDELP CAGGCISASDV+AMLDDSTFDLDVFCPMYTCDGNTDIPSSECLSDDPVENLHQHGG APGAQISVFDVSYVGTE+YAS+AGNDLWYSAMETGAKIHTNSWGGATLCQLTE EY+YD FMYENPEHLLIF+AGNDGGYEDIPNREACTVGSPALGKNSLAVGATSSGPFPGTRTGDDGNL YEE+GLTDYSPEGYPWICL P+LGTPSASTEPAGVDTVA+FSSQGPTLDGRIKPDVVAPGDQVASASSDGTD HSCKL
Sbjct:  397 YSFPFDMSRRKIVQYIQL---FKTPDQGSTSSGQDSFTYVSDYLGDPYDYDDSEYISYDDYSGEYYSDT-------------------------------------------------W-------------------SEDSFCAGV----------------LDDLSYHFSYLFDDTSYSSSSTSPSFTGGFHKDTVGGHGTWTAGSAAGAISAQSILEEEACYGDELPGCAGGCISASDVDAMLDDSTFDLDVFCPMYTCDGNTDIPSSECLSDDPVENLHQHGGVAPGAQISVFDVSYVGTEIYASFAGNDLWYSAMETGAKIHTNSWGGATLCQLTEDEYLYDTFMYENPEHLLIFAAGNDGGYEDIPNREACTVGSPALGKNSLAVGATSSGPFPGTRTGDDGNLVYEELGLTDYSPEGYPWICLTPFLGTPSASTEPAGVDTVAFFSSQGPTLDGRIKPDVVAPGDQVASASSDGTDGHSCKL 798          
BLAST of EsuBft3723_3a-0001 vs. uniprot
Match: D8LU20_ECTSI (Subtilisin n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LU20_ECTSI)

HSP 1 Score: 576 bits (1484), Expect = 8.370e-194
Identity = 311/495 (62.83%), Postives = 343/495 (69.29%), Query Frame = 0
Query:   15 FSTPYKCNGCNVVTYRAVHPAVKTPDQGSTSSGQDSFTYFSDYF----GDPYDYDDSEYISHDDYSGEYFSDTVVPALKTLILSQLPAATDSRSTARVLTDNLFVEKASGLTDAEVRQAVKTWLSVEDDPEVLEEEIAMELQAVDEQLAGVTLDDDVPSEEEDDDGGLDDLAYHLSYLYDDTSYASSSTSPSF--TGGFHKDTIGGHGTSTAGSAAGAISSQSSLEEEACYGDELPACAGGCISASDVEAMLDDSTFDLDVFCPMYTCDGNTDIPSSECLSDDPVENLHQHGGAAPGAQISVFDVSYVGTEVYASWAGNDLWYSAMETGAKIHTNSWGGATLCQLTEKEYMYDMFMYENPEHLLIFSAGNDGGYEDIPNREACTVGSPALGKNSLAVGATSSGPFPGTRTGDDGNLFYEEVGLTDYSPEGYPWICLMPYLGTPSASTEPAGVDTVAYFSSQGPTLDGRIKPDVVAPGDQVASASSDGTDEHSCKL 503
            +S P+  +   +V Y  +    KTPDQGSTSSGQ+SFTY SDY     GD +D DDSEY S+DDYSGEY SDT                                                 W          EE    ++                          LDDL+YH S L+DDTSY+ SS S S   TGGF KD +GGHGT TAG AAGAIS+QSSL+EEACYGDELP CAGGCISASDV+AMLD+ +FDLDVFCPMY CDGNTDIPSSECLSDDPVENLHQHGG APGAQIS+ D SY G++ +A +AGN LWYSAMETGAKIH+NSWG   LCQLTE EY+YD FMYENPEHLLIF+AGN GG EDIPN E CT+GSPALGKN LAVGATSSGP  GTRTG+DGNL YEE G+TDYSPEGYPWICL P LGTPSAS EPAGVDTVA+FSSQGPTLDGRIKPDVVAPGDQVASASSDGTD HSC+L
Sbjct:  350 YSFPFDMSRRKIVQYIQM---FKTPDQGSTSSGQNSFTYASDYIRDYLGDAFDDDDSEYNSYDDYSGEYSSDT-------------------------------------------------WS---------EESFCAQV--------------------------LDDLSYHFSSLFDDTSYSPSSASSSISSTGGFDKDIVGGHGTWTAGCAAGAISAQSSLQEEACYGDELPGCAGGCISASDVDAMLDNGSFDLDVFCPMYNCDGNTDIPSSECLSDDPVENLHQHGGVAPGAQISMLDASYTGSDSFALFAGNMLWYSAMETGAKIHSNSWGYDMLCQLTENEYLYDSFMYENPEHLLIFAAGNHGGNEDIPNHEGCTIGSPALGKNILAVGATSSGPSRGTRTGEDGNLVYEEFGMTDYSPEGYPWICLSPSLGTPSASMEPAGVDTVAFFSSQGPTLDGRIKPDVVAPGDQVASASSDGTDGHSCRL 757          
BLAST of EsuBft3723_3a-0001 vs. uniprot
Match: A0A6H5K229_9PHAE (Subtilisin n=2 Tax=Ectocarpus TaxID=2879 RepID=A0A6H5K229_9PHAE)

HSP 1 Score: 225 bits (573), Expect = 1.390e-60
Identity = 142/304 (46.71%), Postives = 173/304 (56.91%), Query Frame = 0
Query:  207 FHKDTIGGHGTSTAGSAAGAISSQSSLEEEACYGDELPACAGGCISASD-----VEAMLDDSTFDLDVFCPMYTCDGNTDIPSSECLSDDPVENLHQHGGAAPGAQISVFDVSYVGTEVYA--SWAGNDLWYSAMETGAKIHTNSWGGATLCQLTEKEYMYDMFMYENPEHLLIFSAGNDGGYEDIPNREACTVGSPALGKNSLAVGATSSGPFPGTRTGDDGNLFYEEVGLTDYSPEGYPWICLMPYLGTPSASTEPAGVDTVAYFSSQGPTLDGRIKPDVVAPGDQVASASSDGTDEHSCKL 503
            F +D   GHGT TAGSAAGA  +  + E   C GDE   C GGCI  S+     V   +  +  DLD  CPMY CDG+TD     CLSD+  E L ++GG A GA++S+FDV +   EV+      GN +W   +E G K+H+ S GG TLC   E E +YD FMYENPE+LLIF+AGNDG  E    R  CT+ SPA+GKN LAVGA++SG         D  +   E G                           AG+D VA FSS G T DGRIKP+VVAPGD V SA+SDGTD HSC+L
Sbjct:  468 FGQDDSAGHGTHTAGSAAGATLNDPA-ETLTCSGDETLGCVGGCIDESETTDDLVSYAIQIALPDLDRLCPMYGCDGSTD----PCLSDEVDETLTKNGGMAQGAKLSIFDVFF---EVFGLIEVLGNGVWEPCLEAGCKVHSLSLGGDTLCTPGESEILYDDFMYENPENLLIFAAGNDGETE----RSVCTMASPAIGKNVLAVGASTSG---------DTRVISTEGG---------------------------AGIDMVADFSSYGFTTDGRIKPEVVAPGDMVYSAASDGTDSHSCRL 723          
BLAST of EsuBft3723_3a-0001 vs. uniprot
Match: A0A2I6U1R2_SACJA (Subtilisin (Fragment) n=1 Tax=Saccharina japonica TaxID=88149 RepID=A0A2I6U1R2_SACJA)

HSP 1 Score: 223 bits (568), Expect = 2.650e-60
Identity = 135/305 (44.26%), Postives = 171/305 (56.07%), Query Frame = 0
Query:  209 KDTIGGHGTSTAGSAAGAISSQSSLEEEACYGDELPACAGGCISAS------DVEAMLDD-STFDLDV--FCPMYTCDGNTDIPSSECLSDDPVENLHQHGGAAPGAQISVFDVSYVGTEVYASWAGNDLWYSAMETGAKIHTNSWGGATLCQLTEKEYMYDMFMYENPEHLLIFSAGNDGGYEDIPNREACTVGSPALGKNSLAVGATSSGPFPGTRTGDDGNLFYEEVGLTDYSPEGYPWICLMPYLGTPSASTEPAGVDTVAYFSSQGPTLDGRIKPDVVAPGDQVASASSDGTDEHSCKLA 504
            +D + GHGT  AGSA GA     +     C G E+P C GGCI A       D+  ++ + S +D+D+   CPM  CD  TD     CLSDD  + L  HGG A GA+++ FDV   G+     + GN LW   +E G KIH+ S G    C + EK+ +YD FMY NPE+L++ +AGN G  ED   R  CT+ SPA+GKN+LAVGATSSG    T+T  DG+L     G  D                          VDTVA FSS GPT DGRIKP+VVAPGD + SA SDG+D HSC+L+
Sbjct:  413 RDDVEGHGTHCAGSALGATIRTPAETPTTCSGTEVPGCVGGCIDADRTSWGDDLFTLMPEYSPYDIDIDRLCPMIDCDDATD---PRCLSDDVGQTLADHGGMAQGAKLAFFDVFADGSSG-THFVGNGLWEPCLEAGCKIHSMSIGADFECSVCEKDVLYDDFMYNNPENLVVVAAGNAGDTED--GRTVCTMYSPAIGKNALAVGATSSGETRITKTSADGDLIDGTNGYAD--------------------------VDTVAQFSSYGPTQDGRIKPEVVAPGDAIYSARSDGSDNHSCRLS 685          
BLAST of EsuBft3723_3a-0001 vs. uniprot
Match: A0A2I6U1S5_SACJA (Subtilisin n=1 Tax=Saccharina japonica TaxID=88149 RepID=A0A2I6U1S5_SACJA)

HSP 1 Score: 215 bits (548), Expect = 2.480e-57
Identity = 132/308 (42.86%), Postives = 168/308 (54.55%), Query Frame = 0
Query:  206 GFHKDTIGGHGTSTAGSAAGAISSQSSLEEEACYGDELPACAGGCISASDVE-------AMLDDSTF--DLDVFCPMYTCDGNTDIPSSECLSDDPVENLHQHGGAAPGAQISVFDVSYVGTEVYASWAGNDLWYSAMETGAKIHTNSWGGATLCQLTEKEYMYDMFMYENPEHLLIFSAGNDGGYEDIPNREACTVGSPALGKNSLAVGATSSGPFPGTRTGDDGNLFYEEVGLTDYSPEGYPWICLMPYLGTPSASTEPAGVDTVAYFSSQGPTLDGRIKPDVVAPGDQVASASSDGTDEHSCKLA 504
            GF  D +  HGT TAGSA GA     +     C G E+P C GGCI A            M ++S++  D+D  CPM  CD   +     CLSD   + L  HGG A GA++S FD+ Y G  ++  + GN LW   +E G KIH+ S+G    C + + + +YD FMY NPE+L++ +AGN G  ED   R  CT+ SPA+GKN+LAVGATSSG      T  DG+      G +D                          VDTVA FSS GPT DGRIKP+VVAPGD V SA SDG+D HSC+L+
Sbjct:  406 GFQGDDVDDHGTHTAGSALGATIRTPAETPTTCSGTEVPGCVGGCIDADRTSWGDDLLTLMPENSSYVADIDRICPMLGCDEENE---PWCLSDGVEQTLADHGGMAQGAKLSFFDI-YDGNMLFEQFIGNGLWEPCLEAGCKIHSLSFGADLECAVRDTDLLYDDFMYNNPENLVVVAAGNAGEIED--GRTVCTMYSPAIGKNALAVGATSSGETRVGVTSADGDRADGTNGYSD--------------------------VDTVAVFSSYGPTQDGRIKPEVVAPGDAVYSARSDGSDVHSCRLS 681          
BLAST of EsuBft3723_3a-0001 vs. uniprot
Match: D8LBZ2_ECTSI (Subtilisin (Fragment) n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LBZ2_ECTSI)

HSP 1 Score: 208 bits (529), Expect = 2.590e-56
Identity = 127/315 (40.32%), Postives = 164/315 (52.06%), Query Frame = 0
Query:  207 FHKDTIGGHGTSTAGSAAGAISSQSSLEE-----EACYGDELPACAGGCISASDVEAMLDDST-------------FDLDVFCPMYTCDGNTDIPSSECLSDDPVENLHQHGGAAPGAQISVFDVSYVGTEVYASWAGNDLWYSAMETGAKIHTNSWGGATLCQLTEKEYMYDMFMYENPEHLLIFSAGNDGGYEDIPNREACTVGSPALGKNSLAVGATSSGPFPGTRTGDDGNLFYEEVGLTDYSPEGYPWICLMPYLGTPSASTEPAGVDTVAYFSSQGPTLDGRIKPDVVAPGDQVASASSDGTDEHSCKL 503
            F ++  GGHGT TAGSAAGA+ S  S  +     E C      +C GGCI   D     D  T              DLD  CP   CD   D P   CL DD  E L QHGG A GAQ+++FD+    T +    AGN +W  AME G+K+ +NSWG    C+    + +YD FMY N + LL+F+AGNDG          CT+GSPA+ KN LAVGA++SGP   + T +DG+    +  + D                          +D +AYFSS GPTLDGRIKP+VVAPGD + SA+  G   ++C+L
Sbjct:  283 FAENYAGGHGTHTAGSAAGAVVSSPSYSQTGDFTEVCEEGRELSCVGGCIDVDDTFGGDDLITTWDYQYFWAGYEAIDLDRLCPRLDCD---DRPEEYCLGDDEAETLQQHGGVARGAQLAIFDILDDYTGIGIEMAGNGMWEPAMEAGSKLSSNSWGIYAYCEYLSYDVLYDDFMYRNQDQLLLFAAGNDG-----EEWSGCTMGSPAVAKNVLAVGASTSGPGRLSFTDEDGDPTDGDSRVQD--------------------------IDAMAYFSSFGPTLDGRIKPEVVAPGDMIYSAAGGGDGNYTCRL 563          
BLAST of EsuBft3723_3a-0001 vs. uniprot
Match: D8LNG6_ECTSI (Subtilisin n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LNG6_ECTSI)

HSP 1 Score: 209 bits (533), Expect = 3.230e-55
Identity = 139/322 (43.17%), Postives = 176/322 (54.66%), Query Frame = 0
Query:  192 TSYASSSTSPSFTGG----FHKDTIGGHGTSTAGSAAGAISSQSSLEEEACYGDELPACAGGCISASDVEAML------DDSTFDLDVFCPMYTCDGNTDIPSSECLSDDPVENLHQHGGAAPGAQISVFDVSYVGTEVYASWAGNDLWYSAMETGAKIHTNSWGGATLCQLTEKEYMYDMFMYENPEHLLIFSAGNDGGYEDIPNREACTVGSPALGKNSLAVGATSSGPFPGTRTGDDGNLFYEEVGLTDYSPEGYPWICLMPYLGTPSASTEPAGVDTVAYFSSQGPTLDGRIKPDVVAPGDQVASASSDGTDEHSCKL 503
            +SY+S  T+  F GG    + +    GHGT TAGSAAGA  +  + E   C   +  +C G CI   D    L       D T DLD  CPMY CD +TD   + CLSDD  E L  +GG A GA++++FDV Y G +  +S  GN+LW    E G K+H+NS G    C +   + + D FMYEN E+LLIF+AGN+GG E    R  CT+ +P +GKNSLA+GA++SG    TR           VG T                          G+DTVA FSS G T D RIKP+VVAPGD V SA+SDGTD HSC+L
Sbjct:  412 SSYSSDETATIFNGGDFSYYPERRKAGHGTHTAGSAAGATLNDPA-ETITCNVTDTLSCVGACIDLLDATDDLVSISSQTDGT-DLDRLCPMYGCDNSTD---TTCLSDDVPETLSNNGGMAQGAKLAIFDVFYGGYDASSS-IGNNLWEVCTEAGCKLHSNSLGADFECSIASWDILNDQFMYENAENLLIFAAGNEGGIE----RSTCTINTPGIGKNSLAIGASTSGD---TRV-------ISTVGAT--------------------------GIDTVAEFSSWGFTTDDRIKPEVVAPGDSVYSAASDGTDTHSCRL 687          
BLAST of EsuBft3723_3a-0001 vs. uniprot
Match: A0A6H5JKA8_9PHAE (Subtilisin n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JKA8_9PHAE)

HSP 1 Score: 209 bits (531), Expect = 1.060e-54
Identity = 127/315 (40.32%), Postives = 164/315 (52.06%), Query Frame = 0
Query:  207 FHKDTIGGHGTSTAGSAAGAISSQSSLEE-----EACYGDELPACAGGCISASDVEAMLDDST-------------FDLDVFCPMYTCDGNTDIPSSECLSDDPVENLHQHGGAAPGAQISVFDVSYVGTEVYASWAGNDLWYSAMETGAKIHTNSWGGATLCQLTEKEYMYDMFMYENPEHLLIFSAGNDGGYEDIPNREACTVGSPALGKNSLAVGATSSGPFPGTRTGDDGNLFYEEVGLTDYSPEGYPWICLMPYLGTPSASTEPAGVDTVAYFSSQGPTLDGRIKPDVVAPGDQVASASSDGTDEHSCKL 503
            F +D  GGHGT TAGSAAGA+ S  S  +     E C      +C GGCI   D     D  T              DLD  CP   CD   D+P   CL DD  E L QHGG A GAQ+++FD+    T V    AGN +W  AME G+K+ +NSWG    C     + ++D FMY N + LL+F+AGNDG      +   CT+G+PAL KN LAVGA++SGP   + T +DG+    +  + D                          +D +AYFSS GPTLD RIKP+VVAPGD + SA+  G   ++C+L
Sbjct:  566 FAEDDAGGHGTHTAGSAAGAVVSSPSYSQTGDFTEVCEEGRELSCVGGCIDVDDPFGEDDLITTWDYQYFWAGYEAIDLDRLCPRLDCD---DLPEEYCLGDDEAETLQQHGGVARGAQLAIFDIINDYTGVGIDMAGNGMWEPAMEAGSKLSSNSWGIYAYCDYLSLDVLFDDFMYRNQDQLLLFAAGNDGD-----DWSGCTMGTPALAKNVLAVGASTSGPGRLSFTDEDGDPTDGDSRVQD--------------------------IDAMAYFSSFGPTLDNRIKPEVVAPGDMIYSAAGSGDGNYTCRL 846          
BLAST of EsuBft3723_3a-0001 vs. uniprot
Match: A0A6H5KDF8_9PHAE (Subtilisin n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5KDF8_9PHAE)

HSP 1 Score: 1049 bits (2712), Expect = 0.000e+0
Identity = 519/519 (100.00%), Postives = 519/519 (100.00%), Query Frame = 1
Query:    1 MSQVNSKPADYPVQFSTPYKCNGCNVVTYRAVHPAVKTPDQGSTSSGQDSFTYFSDYFGDPYDYDDSEYISHDDYSGEYFSDTVVPALKTLILSQLPAATDSRSTARVLTDNLFVEKASGLTDAEVRQAVKTWLSVEDDPEVLEEEIAMELQAVDEQLAGVTLDDDVPSEEEDDDGGLDDLAYHLSYLYDDTSYASSSTSPSFTGGFHKDTIGGHGTSTAGSAAGAISSQSSLEEEACYGDELPACAGGCISASDVEAMLDDSTFDLDVFCPMYTCDGNTDIPSSECLSDDPVENLHQHGGAAPGAQISVFDVSYVGTEVYASWAGNDLWYSAMETGAKIHTNSWGGATLCQLTEKEYMYDMFMYENPEHLLIFSAGNDGGYEDIPNREACTVGSPALGKNSLAVGATSSGPFPGTRTGDDGNLFYEEVGLTDYSPEGYPWICLMPYLGTPSASTEPAGVDTVAYFSSQGPTLDGRIKPDVVAPGDQVASASSDGTDEHSCKLAGPPLPAPWLPVPLPW 1557
            MSQVNSKPADYPVQFSTPYKCNGCNVVTYRAVHPAVKTPDQGSTSSGQDSFTYFSDYFGDPYDYDDSEYISHDDYSGEYFSDTVVPALKTLILSQLPAATDSRSTARVLTDNLFVEKASGLTDAEVRQAVKTWLSVEDDPEVLEEEIAMELQAVDEQLAGVTLDDDVPSEEEDDDGGLDDLAYHLSYLYDDTSYASSSTSPSFTGGFHKDTIGGHGTSTAGSAAGAISSQSSLEEEACYGDELPACAGGCISASDVEAMLDDSTFDLDVFCPMYTCDGNTDIPSSECLSDDPVENLHQHGGAAPGAQISVFDVSYVGTEVYASWAGNDLWYSAMETGAKIHTNSWGGATLCQLTEKEYMYDMFMYENPEHLLIFSAGNDGGYEDIPNREACTVGSPALGKNSLAVGATSSGPFPGTRTGDDGNLFYEEVGLTDYSPEGYPWICLMPYLGTPSASTEPAGVDTVAYFSSQGPTLDGRIKPDVVAPGDQVASASSDGTDEHSCKLAGPPLPAPWLPVPLPW
Sbjct:    1 MSQVNSKPADYPVQFSTPYKCNGCNVVTYRAVHPAVKTPDQGSTSSGQDSFTYFSDYFGDPYDYDDSEYISHDDYSGEYFSDTVVPALKTLILSQLPAATDSRSTARVLTDNLFVEKASGLTDAEVRQAVKTWLSVEDDPEVLEEEIAMELQAVDEQLAGVTLDDDVPSEEEDDDGGLDDLAYHLSYLYDDTSYASSSTSPSFTGGFHKDTIGGHGTSTAGSAAGAISSQSSLEEEACYGDELPACAGGCISASDVEAMLDDSTFDLDVFCPMYTCDGNTDIPSSECLSDDPVENLHQHGGAAPGAQISVFDVSYVGTEVYASWAGNDLWYSAMETGAKIHTNSWGGATLCQLTEKEYMYDMFMYENPEHLLIFSAGNDGGYEDIPNREACTVGSPALGKNSLAVGATSSGPFPGTRTGDDGNLFYEEVGLTDYSPEGYPWICLMPYLGTPSASTEPAGVDTVAYFSSQGPTLDGRIKPDVVAPGDQVASASSDGTDEHSCKLAGPPLPAPWLPVPLPW 519          
BLAST of EsuBft3723_3a-0001 vs. uniprot
Match: A0A6H5JQ52_9PHAE (Subtilisin n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JQ52_9PHAE)

HSP 1 Score: 696 bits (1797), Expect = 2.310e-243
Identity = 358/484 (73.97%), Postives = 374/484 (77.27%), Query Frame = 1
Query:  109 KTPDQGSTSSGQDSFTYFSDYFGDPYDYDDSEYISHDDYSGEYFSDTVVPALKTLILSQLPAATDSRSTARVLTDNLFVEKASGLTDAEVRQAVKTWLSVEDDPEVLEEEIAMELQAVDEQLAGVTLDDDVPSEEEDDDGGLDDLAYHLSYLYDDTSYASSSTSPSFTGGFHKDTIGGHGTSTAGSAAGAISSQSSLEEEACYGDELPACAGGCISASDVEAMLDDSTFDLDVFCPMYTCDGNTDIPSSECLSDDPVENLHQHGGAAPGAQISVFDVSYVGTEVYASWAGNDLWYSAMETGAKIHTNSWGGATLCQLTEKEYMYDMFMYENPEHLLIFSAGNDGGYEDIPNREACTVGSPALGKNSLAVGATSSGPFPGTRTGDDGNLFYEEVGLTDYSPEGYPWICLMPYLGTPSASTEPAGVDTVAYFSSQGPTLDGRIKPDVVAPGDQV-ASASSDGTDEHSCKLAGPPLPAPWLPVPLPW 1557
            KTP QGSTSSGQDSFT+FSD+ GDP+DYDDSEYIS+ DYSGEY+SDT                                                 W                                   SE+    GGLDDL+YHLSYLYDDTSYAS STSPSFTGGFH+DTIGGHGT T+GSAAGAISSQSSL+EEACYGDELP CAGGCISASDV+AMLDDSTF L VFCPMYTCDGN DIPSSECLSDDPVE+LHQHGG APGAQISVFD SYVGTEVYASWAGNDLWYSAMETGAKIHTNSWGGATLCQLTEKEYMYD FMYENPEHLLIFSAGNDGGY+DIPNREACTVGSPALGKN LAVGATSSGPFPGTRTGDDGNLF EEVG+TDYSPEGYPWICLMP LG P ASTEPAGVDTV YFSSQGPTLDG+IKP VVAPG+QV ASASSDGTDEHSCKLAGPP PAPW PVPLPW
Sbjct:  328 KTPHQGSTSSGQDSFTFFSDFIGDPFDYDDSEYISYGDYSGEYYSDT-------------------------------------------------W-----------------------------------SEDSFCAGGLDDLSYHLSYLYDDTSYASPSTSPSFTGGFHQDTIGGHGTGTSGSAAGAISSQSSLKEEACYGDELPGCAGGCISASDVDAMLDDSTFHLVVFCPMYTCDGNIDIPSSECLSDDPVEDLHQHGGVAPGAQISVFDFSYVGTEVYASWAGNDLWYSAMETGAKIHTNSWGGATLCQLTEKEYMYDTFMYENPEHLLIFSAGNDGGYDDIPNREACTVGSPALGKNILAVGATSSGPFPGTRTGDDGNLFCEEVGVTDYSPEGYPWICLMPVLGAPPASTEPAGVDTVGYFSSQGPTLDGQIKPGVVAPGNQVVASASSDGTDEHSCKLAGPPFPAPWSPVPLPW 727          
BLAST of EsuBft3723_3a-0001 vs. uniprot
Match: D8LSW0_ECTSI (Subtilisin n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LSW0_ECTSI)

HSP 1 Score: 686 bits (1769), Expect = 4.730e-235
Identity = 355/489 (72.60%), Postives = 376/489 (76.89%), Query Frame = 1
Query:   43 FSTPYKCNGCNVVTYRAVHPAVKTPDQGSTSSGQDSFTYFSDYFGDPYDYDDSEYISHDDYSGEYFSDTVVPALKTLILSQLPAATDSRSTARVLTDNLFVEKASGLTDAEVRQAVKTWLSVEDDPEVLEEEIAMELQAVDEQLAGVTLDDDVPSEEEDDDGGLDDLAYHLSYLYDDTSYASSSTSPSFTGGFHKDTIGGHGTSTAGSAAGAISSQSSLEEEACYGDELPACAGGCISASDVEAMLDDSTFDLDVFCPMYTCDGNTDIPSSECLSDDPVENLHQHGGAAPGAQISVFDVSYVGTEVYASWAGNDLWYSAMETGAKIHTNSWGGATLCQLTEKEYMYDMFMYENPEHLLIFSAGNDGGYEDIPNREACTVGSPALGKNSLAVGATSSGPFPGTRTGDDGNLFYEEVGLTDYSPEGYPWICLMPYLGTPSASTEPAGVDTVAYFSSQGPTLDGRIKPDVVAPGDQVASASSDGTDEHSCKL 1509
            +S P+  +   +V Y  +    KTPDQGSTSSGQDSFTY SDY GDPYDYDDSEYIS+DDYSGEY+SDT                                                 W                   + D   AGV                LDDL+YH SYL+DDTSY+SSSTSPSFTGGFHKDT+GGHGT TAGSAAGAIS+QS LEEEACYGDELP CAGGCISASDV+AMLDDSTFDLDVFCPMYTCDGNTDIPSSECLSDDPVENLHQHGG APGAQISVFDVSYVGTE+YAS+AGNDLWYSAMETGAKIHTNSWGGATLCQLTE EY+YD FMYENPEHLLIF+AGNDGGYEDIPNREACTVGSPALGKNSLAVGATSSGPFPGTRTGDDGNL YEE+GLTDYSPEGYPWICL P+LGTPSASTEPAGVDTVA+FSSQGPTLDGRIKPDVVAPGDQVASASSDGTD HSCKL
Sbjct:  397 YSFPFDMSRRKIVQYIQL---FKTPDQGSTSSGQDSFTYVSDYLGDPYDYDDSEYISYDDYSGEYYSDT-------------------------------------------------W-------------------SEDSFCAGV----------------LDDLSYHFSYLFDDTSYSSSSTSPSFTGGFHKDTVGGHGTWTAGSAAGAISAQSILEEEACYGDELPGCAGGCISASDVDAMLDDSTFDLDVFCPMYTCDGNTDIPSSECLSDDPVENLHQHGGVAPGAQISVFDVSYVGTEIYASFAGNDLWYSAMETGAKIHTNSWGGATLCQLTEDEYLYDTFMYENPEHLLIFAAGNDGGYEDIPNREACTVGSPALGKNSLAVGATSSGPFPGTRTGDDGNLVYEELGLTDYSPEGYPWICLTPFLGTPSASTEPAGVDTVAFFSSQGPTLDGRIKPDVVAPGDQVASASSDGTDGHSCKL 798          
BLAST of EsuBft3723_3a-0001 vs. uniprot
Match: D8LU20_ECTSI (Subtilisin n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LU20_ECTSI)

HSP 1 Score: 576 bits (1485), Expect = 7.290e-193
Identity = 311/495 (62.83%), Postives = 343/495 (69.29%), Query Frame = 1
Query:   43 FSTPYKCNGCNVVTYRAVHPAVKTPDQGSTSSGQDSFTYFSDYF----GDPYDYDDSEYISHDDYSGEYFSDTVVPALKTLILSQLPAATDSRSTARVLTDNLFVEKASGLTDAEVRQAVKTWLSVEDDPEVLEEEIAMELQAVDEQLAGVTLDDDVPSEEEDDDGGLDDLAYHLSYLYDDTSYASSSTSPSF--TGGFHKDTIGGHGTSTAGSAAGAISSQSSLEEEACYGDELPACAGGCISASDVEAMLDDSTFDLDVFCPMYTCDGNTDIPSSECLSDDPVENLHQHGGAAPGAQISVFDVSYVGTEVYASWAGNDLWYSAMETGAKIHTNSWGGATLCQLTEKEYMYDMFMYENPEHLLIFSAGNDGGYEDIPNREACTVGSPALGKNSLAVGATSSGPFPGTRTGDDGNLFYEEVGLTDYSPEGYPWICLMPYLGTPSASTEPAGVDTVAYFSSQGPTLDGRIKPDVVAPGDQVASASSDGTDEHSCKL 1509
            +S P+  +   +V Y  +    KTPDQGSTSSGQ+SFTY SDY     GD +D DDSEY S+DDYSGEY SDT                                                 W          EE    ++                          LDDL+YH S L+DDTSY+ SS S S   TGGF KD +GGHGT TAG AAGAIS+QSSL+EEACYGDELP CAGGCISASDV+AMLD+ +FDLDVFCPMY CDGNTDIPSSECLSDDPVENLHQHGG APGAQIS+ D SY G++ +A +AGN LWYSAMETGAKIH+NSWG   LCQLTE EY+YD FMYENPEHLLIF+AGN GG EDIPN E CT+GSPALGKN LAVGATSSGP  GTRTG+DGNL YEE G+TDYSPEGYPWICL P LGTPSAS EPAGVDTVA+FSSQGPTLDGRIKPDVVAPGDQVASASSDGTD HSC+L
Sbjct:  350 YSFPFDMSRRKIVQYIQM---FKTPDQGSTSSGQNSFTYASDYIRDYLGDAFDDDDSEYNSYDDYSGEYSSDT-------------------------------------------------WS---------EESFCAQV--------------------------LDDLSYHFSSLFDDTSYSPSSASSSISSTGGFDKDIVGGHGTWTAGCAAGAISAQSSLQEEACYGDELPGCAGGCISASDVDAMLDNGSFDLDVFCPMYNCDGNTDIPSSECLSDDPVENLHQHGGVAPGAQISMLDASYTGSDSFALFAGNMLWYSAMETGAKIHSNSWGYDMLCQLTENEYLYDSFMYENPEHLLIFAAGNHGGNEDIPNHEGCTIGSPALGKNILAVGATSSGPSRGTRTGEDGNLVYEEFGMTDYSPEGYPWICLSPSLGTPSASMEPAGVDTVAFFSSQGPTLDGRIKPDVVAPGDQVASASSDGTDGHSCRL 757          
BLAST of EsuBft3723_3a-0001 vs. uniprot
Match: A0A6H5K229_9PHAE (Subtilisin n=2 Tax=Ectocarpus TaxID=2879 RepID=A0A6H5K229_9PHAE)

HSP 1 Score: 225 bits (574), Expect = 5.170e-60
Identity = 142/304 (46.71%), Postives = 173/304 (56.91%), Query Frame = 1
Query:  619 FHKDTIGGHGTSTAGSAAGAISSQSSLEEEACYGDELPACAGGCISASD-----VEAMLDDSTFDLDVFCPMYTCDGNTDIPSSECLSDDPVENLHQHGGAAPGAQISVFDVSYVGTEVYA--SWAGNDLWYSAMETGAKIHTNSWGGATLCQLTEKEYMYDMFMYENPEHLLIFSAGNDGGYEDIPNREACTVGSPALGKNSLAVGATSSGPFPGTRTGDDGNLFYEEVGLTDYSPEGYPWICLMPYLGTPSASTEPAGVDTVAYFSSQGPTLDGRIKPDVVAPGDQVASASSDGTDEHSCKL 1509
            F +D   GHGT TAGSAAGA  +  + E   C GDE   C GGCI  S+     V   +  +  DLD  CPMY CDG+TD     CLSD+  E L ++GG A GA++S+FDV +   EV+      GN +W   +E G K+H+ S GG TLC   E E +YD FMYENPE+LLIF+AGNDG  E    R  CT+ SPA+GKN LAVGA++SG         D  +   E G                           AG+D VA FSS G T DGRIKP+VVAPGD V SA+SDGTD HSC+L
Sbjct:  468 FGQDDSAGHGTHTAGSAAGATLNDPA-ETLTCSGDETLGCVGGCIDESETTDDLVSYAIQIALPDLDRLCPMYGCDGSTD----PCLSDEVDETLTKNGGMAQGAKLSIFDVFF---EVFGLIEVLGNGVWEPCLEAGCKVHSLSLGGDTLCTPGESEILYDDFMYENPENLLIFAAGNDGETE----RSVCTMASPAIGKNVLAVGASTSG---------DTRVISTEGG---------------------------AGIDMVADFSSYGFTTDGRIKPEVVAPGDMVYSAASDGTDSHSCRL 723          
BLAST of EsuBft3723_3a-0001 vs. uniprot
Match: A0A2I6U1R2_SACJA (Subtilisin (Fragment) n=1 Tax=Saccharina japonica TaxID=88149 RepID=A0A2I6U1R2_SACJA)

HSP 1 Score: 223 bits (569), Expect = 9.650e-60
Identity = 135/305 (44.26%), Postives = 171/305 (56.07%), Query Frame = 1
Query:  625 KDTIGGHGTSTAGSAAGAISSQSSLEEEACYGDELPACAGGCISAS------DVEAMLDD-STFDLDV--FCPMYTCDGNTDIPSSECLSDDPVENLHQHGGAAPGAQISVFDVSYVGTEVYASWAGNDLWYSAMETGAKIHTNSWGGATLCQLTEKEYMYDMFMYENPEHLLIFSAGNDGGYEDIPNREACTVGSPALGKNSLAVGATSSGPFPGTRTGDDGNLFYEEVGLTDYSPEGYPWICLMPYLGTPSASTEPAGVDTVAYFSSQGPTLDGRIKPDVVAPGDQVASASSDGTDEHSCKLA 1512
            +D + GHGT  AGSA GA     +     C G E+P C GGCI A       D+  ++ + S +D+D+   CPM  CD  TD     CLSDD  + L  HGG A GA+++ FDV   G+     + GN LW   +E G KIH+ S G    C + EK+ +YD FMY NPE+L++ +AGN G  ED   R  CT+ SPA+GKN+LAVGATSSG    T+T  DG+L     G  D                          VDTVA FSS GPT DGRIKP+VVAPGD + SA SDG+D HSC+L+
Sbjct:  413 RDDVEGHGTHCAGSALGATIRTPAETPTTCSGTEVPGCVGGCIDADRTSWGDDLFTLMPEYSPYDIDIDRLCPMIDCDDATD---PRCLSDDVGQTLADHGGMAQGAKLAFFDVFADGSSG-THFVGNGLWEPCLEAGCKIHSMSIGADFECSVCEKDVLYDDFMYNNPENLVVVAAGNAGDTED--GRTVCTMYSPAIGKNALAVGATSSGETRITKTSADGDLIDGTNGYAD--------------------------VDTVAQFSSYGPTQDGRIKPEVVAPGDAIYSARSDGSDNHSCRLS 685          
BLAST of EsuBft3723_3a-0001 vs. uniprot
Match: A0A2I6U1S5_SACJA (Subtilisin n=1 Tax=Saccharina japonica TaxID=88149 RepID=A0A2I6U1S5_SACJA)

HSP 1 Score: 216 bits (549), Expect = 8.680e-57
Identity = 132/308 (42.86%), Postives = 168/308 (54.55%), Query Frame = 1
Query:  616 GFHKDTIGGHGTSTAGSAAGAISSQSSLEEEACYGDELPACAGGCISASDVE-------AMLDDSTF--DLDVFCPMYTCDGNTDIPSSECLSDDPVENLHQHGGAAPGAQISVFDVSYVGTEVYASWAGNDLWYSAMETGAKIHTNSWGGATLCQLTEKEYMYDMFMYENPEHLLIFSAGNDGGYEDIPNREACTVGSPALGKNSLAVGATSSGPFPGTRTGDDGNLFYEEVGLTDYSPEGYPWICLMPYLGTPSASTEPAGVDTVAYFSSQGPTLDGRIKPDVVAPGDQVASASSDGTDEHSCKLA 1512
            GF  D +  HGT TAGSA GA     +     C G E+P C GGCI A            M ++S++  D+D  CPM  CD   +     CLSD   + L  HGG A GA++S FD+ Y G  ++  + GN LW   +E G KIH+ S+G    C + + + +YD FMY NPE+L++ +AGN G  ED   R  CT+ SPA+GKN+LAVGATSSG      T  DG+      G +D                          VDTVA FSS GPT DGRIKP+VVAPGD V SA SDG+D HSC+L+
Sbjct:  406 GFQGDDVDDHGTHTAGSALGATIRTPAETPTTCSGTEVPGCVGGCIDADRTSWGDDLLTLMPENSSYVADIDRICPMLGCDEENE---PWCLSDGVEQTLADHGGMAQGAKLSFFDI-YDGNMLFEQFIGNGLWEPCLEAGCKIHSLSFGADLECAVRDTDLLYDDFMYNNPENLVVVAAGNAGEIED--GRTVCTMYSPAIGKNALAVGATSSGETRVGVTSADGDRADGTNGYSD--------------------------VDTVAVFSSYGPTQDGRIKPEVVAPGDAVYSARSDGSDVHSCRLS 681          
BLAST of EsuBft3723_3a-0001 vs. uniprot
Match: D8LBZ2_ECTSI (Subtilisin (Fragment) n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LBZ2_ECTSI)

HSP 1 Score: 208 bits (530), Expect = 8.410e-56
Identity = 127/315 (40.32%), Postives = 164/315 (52.06%), Query Frame = 1
Query:  619 FHKDTIGGHGTSTAGSAAGAISSQSSLEE-----EACYGDELPACAGGCISASDVEAMLDDST-------------FDLDVFCPMYTCDGNTDIPSSECLSDDPVENLHQHGGAAPGAQISVFDVSYVGTEVYASWAGNDLWYSAMETGAKIHTNSWGGATLCQLTEKEYMYDMFMYENPEHLLIFSAGNDGGYEDIPNREACTVGSPALGKNSLAVGATSSGPFPGTRTGDDGNLFYEEVGLTDYSPEGYPWICLMPYLGTPSASTEPAGVDTVAYFSSQGPTLDGRIKPDVVAPGDQVASASSDGTDEHSCKL 1509
            F ++  GGHGT TAGSAAGA+ S  S  +     E C      +C GGCI   D     D  T              DLD  CP   CD   D P   CL DD  E L QHGG A GAQ+++FD+    T +    AGN +W  AME G+K+ +NSWG    C+    + +YD FMY N + LL+F+AGNDG          CT+GSPA+ KN LAVGA++SGP   + T +DG+    +  + D                          +D +AYFSS GPTLDGRIKP+VVAPGD + SA+  G   ++C+L
Sbjct:  283 FAENYAGGHGTHTAGSAAGAVVSSPSYSQTGDFTEVCEEGRELSCVGGCIDVDDTFGGDDLITTWDYQYFWAGYEAIDLDRLCPRLDCD---DRPEEYCLGDDEAETLQQHGGVARGAQLAIFDILDDYTGIGIEMAGNGMWEPAMEAGSKLSSNSWGIYAYCEYLSYDVLYDDFMYRNQDQLLLFAAGNDG-----EEWSGCTMGSPAVAKNVLAVGASTSGPGRLSFTDEDGDPTDGDSRVQD--------------------------IDAMAYFSSFGPTLDGRIKPEVVAPGDMIYSAAGGGDGNYTCRL 563          
BLAST of EsuBft3723_3a-0001 vs. uniprot
Match: D8LNG6_ECTSI (Subtilisin n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LNG6_ECTSI)

HSP 1 Score: 210 bits (534), Expect = 1.090e-54
Identity = 139/322 (43.17%), Postives = 176/322 (54.66%), Query Frame = 1
Query:  574 TSYASSSTSPSFTGG----FHKDTIGGHGTSTAGSAAGAISSQSSLEEEACYGDELPACAGGCISASDVEAML------DDSTFDLDVFCPMYTCDGNTDIPSSECLSDDPVENLHQHGGAAPGAQISVFDVSYVGTEVYASWAGNDLWYSAMETGAKIHTNSWGGATLCQLTEKEYMYDMFMYENPEHLLIFSAGNDGGYEDIPNREACTVGSPALGKNSLAVGATSSGPFPGTRTGDDGNLFYEEVGLTDYSPEGYPWICLMPYLGTPSASTEPAGVDTVAYFSSQGPTLDGRIKPDVVAPGDQVASASSDGTDEHSCKL 1509
            +SY+S  T+  F GG    + +    GHGT TAGSAAGA  +  + E   C   +  +C G CI   D    L       D T DLD  CPMY CD +TD   + CLSDD  E L  +GG A GA++++FDV Y G +  +S  GN+LW    E G K+H+NS G    C +   + + D FMYEN E+LLIF+AGN+GG E    R  CT+ +P +GKNSLA+GA++SG    TR           VG T                          G+DTVA FSS G T D RIKP+VVAPGD V SA+SDGTD HSC+L
Sbjct:  412 SSYSSDETATIFNGGDFSYYPERRKAGHGTHTAGSAAGATLNDPA-ETITCNVTDTLSCVGACIDLLDATDDLVSISSQTDGT-DLDRLCPMYGCDNSTD---TTCLSDDVPETLSNNGGMAQGAKLAIFDVFYGGYDASSS-IGNNLWEVCTEAGCKLHSNSLGADFECSIASWDILNDQFMYENAENLLIFAAGNEGGIE----RSTCTINTPGIGKNSLAIGASTSGD---TRV-------ISTVGAT--------------------------GIDTVAEFSSWGFTTDDRIKPEVVAPGDSVYSAASDGTDTHSCRL 687          
BLAST of EsuBft3723_3a-0001 vs. uniprot
Match: A0A6H5JKA8_9PHAE (Subtilisin n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JKA8_9PHAE)

HSP 1 Score: 209 bits (532), Expect = 3.640e-54
Identity = 127/315 (40.32%), Postives = 164/315 (52.06%), Query Frame = 1
Query:  619 FHKDTIGGHGTSTAGSAAGAISSQSSLEE-----EACYGDELPACAGGCISASDVEAMLDDST-------------FDLDVFCPMYTCDGNTDIPSSECLSDDPVENLHQHGGAAPGAQISVFDVSYVGTEVYASWAGNDLWYSAMETGAKIHTNSWGGATLCQLTEKEYMYDMFMYENPEHLLIFSAGNDGGYEDIPNREACTVGSPALGKNSLAVGATSSGPFPGTRTGDDGNLFYEEVGLTDYSPEGYPWICLMPYLGTPSASTEPAGVDTVAYFSSQGPTLDGRIKPDVVAPGDQVASASSDGTDEHSCKL 1509
            F +D  GGHGT TAGSAAGA+ S  S  +     E C      +C GGCI   D     D  T              DLD  CP   CD   D+P   CL DD  E L QHGG A GAQ+++FD+    T V    AGN +W  AME G+K+ +NSWG    C     + ++D FMY N + LL+F+AGNDG      +   CT+G+PAL KN LAVGA++SGP   + T +DG+    +  + D                          +D +AYFSS GPTLD RIKP+VVAPGD + SA+  G   ++C+L
Sbjct:  566 FAEDDAGGHGTHTAGSAAGAVVSSPSYSQTGDFTEVCEEGRELSCVGGCIDVDDPFGEDDLITTWDYQYFWAGYEAIDLDRLCPRLDCD---DLPEEYCLGDDEAETLQQHGGVARGAQLAIFDIINDYTGVGIDMAGNGMWEPAMEAGSKLSSNSWGIYAYCDYLSLDVLFDDFMYRNQDQLLLFAAGNDGD-----DWSGCTMGTPALAKNVLAVGASTSGPGRLSFTDEDGDPTDGDSRVQD--------------------------IDAMAYFSSFGPTLDNRIKPEVVAPGDMIYSAAGSGDGNYTCRL 846          
The following BLAST results are available for this feature:
BLAST of EsuBft3723_3a-0001 vs. uniprot
Analysis Date: 2022-09-16 (Diamond blastp: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A6H5KDF8_9PHAE0.000e+0100.00Subtilisin n=1 Tax=Ectocarpus sp. CCAP 1310/34 Tax... [more]
A0A6H5JQ52_9PHAE6.860e-24473.97Subtilisin n=1 Tax=Ectocarpus sp. CCAP 1310/34 Tax... [more]
D8LSW0_ECTSI4.960e-23672.60Subtilisin n=1 Tax=Ectocarpus siliculosus TaxID=28... [more]
D8LU20_ECTSI8.370e-19462.83Subtilisin n=1 Tax=Ectocarpus siliculosus TaxID=28... [more]
A0A6H5K229_9PHAE1.390e-6046.71Subtilisin n=2 Tax=Ectocarpus TaxID=2879 RepID=A0A... [more]
A0A2I6U1R2_SACJA2.650e-6044.26Subtilisin (Fragment) n=1 Tax=Saccharina japonica ... [more]
A0A2I6U1S5_SACJA2.480e-5742.86Subtilisin n=1 Tax=Saccharina japonica TaxID=88149... [more]
D8LBZ2_ECTSI2.590e-5640.32Subtilisin (Fragment) n=1 Tax=Ectocarpus siliculos... [more]
D8LNG6_ECTSI3.230e-5543.17Subtilisin n=1 Tax=Ectocarpus siliculosus TaxID=28... [more]
A0A6H5JKA8_9PHAE1.060e-5440.32Subtilisin n=1 Tax=Ectocarpus sp. CCAP 1310/34 Tax... [more]

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BLAST of EsuBft3723_3a-0001 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A6H5KDF8_9PHAE0.000e+0100.00Subtilisin n=1 Tax=Ectocarpus sp. CCAP 1310/34 Tax... [more]
A0A6H5JQ52_9PHAE2.310e-24373.97Subtilisin n=1 Tax=Ectocarpus sp. CCAP 1310/34 Tax... [more]
D8LSW0_ECTSI4.730e-23572.60Subtilisin n=1 Tax=Ectocarpus siliculosus TaxID=28... [more]
D8LU20_ECTSI7.290e-19362.83Subtilisin n=1 Tax=Ectocarpus siliculosus TaxID=28... [more]
A0A6H5K229_9PHAE5.170e-6046.71Subtilisin n=2 Tax=Ectocarpus TaxID=2879 RepID=A0A... [more]
A0A2I6U1R2_SACJA9.650e-6044.26Subtilisin (Fragment) n=1 Tax=Saccharina japonica ... [more]
A0A2I6U1S5_SACJA8.680e-5742.86Subtilisin n=1 Tax=Saccharina japonica TaxID=88149... [more]
D8LBZ2_ECTSI8.410e-5640.32Subtilisin (Fragment) n=1 Tax=Ectocarpus siliculos... [more]
D8LNG6_ECTSI1.090e-5443.17Subtilisin n=1 Tax=Ectocarpus siliculosus TaxID=28... [more]
A0A6H5JKA8_9PHAE3.640e-5440.32Subtilisin n=1 Tax=Ectocarpus sp. CCAP 1310/34 Tax... [more]

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InterPro
Analysis Name: InterProScan on OGS1.0
Date Performed: 2022-09-29
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036852Peptidase S8/S53 domain superfamilyGENE3D3.40.50.200Peptidase S8/S53 domaincoord: 160..506
e-value: 3.5E-28
score: 100.6
IPR036852Peptidase S8/S53 domain superfamilySUPERFAMILY52743Subtilisin-likecoord: 175..498
IPR000209Peptidase S8/S53 domainPFAMPF00082Peptidase_S8coord: 297..492
e-value: 6.2E-21
score: 74.9
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 485..505
NoneNo IPR availablePANTHERPTHR43399SUBTILISIN-RELATEDcoord: 450..499
NoneNo IPR availablePANTHERPTHR43399:SF1PROPROTEIN CONVERTASE, PUTATIVE-RELATEDcoord: 450..499
NoneNo IPR availablePANTHERPTHR43399:SF1PROPROTEIN CONVERTASE, PUTATIVE-RELATEDcoord: 200..410
NoneNo IPR availablePANTHERPTHR43399SUBTILISIN-RELATEDcoord: 200..410

Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
Scaffold_647contigScaffold_647:198023..202154 +
Analyses
This polypeptide is derived from or has results from the following analyses
Analysis NameDate Performed
InterProScan on OGS1.02022-09-29
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Diamond blastp: OGS1.0 vs UniRef902022-09-16
OGS1.0 of Ectocarpus subulatus male Bft15b2020-06-19
Relationships

This polypeptide derives from the following mRNA feature(s):

Feature NameUnique NameSpeciesTypePosition
EsuBft3723_3a-0001EsuBft3723_3a-0001Ectocarpus subulatus male Bft15bmRNAScaffold_647 198023..202358 +


Sequences
The following sequences are available for this feature:

polypeptide sequence

>EsuBft3723_3 ID=EsuBft3723_3|Name=EsuBft3723_3a-0001|organism=Ectocarpus subulatus male Bft15b|type=polypeptide|length=519bp
MSQVNSKPADYPVQFSTPYKCNGCNVVTYRAVHPAVKTPDQGSTSSGQDS
FTYFSDYFGDPYDYDDSEYISHDDYSGEYFSDTVVPALKTLILSQLPAAT
DSRSTARVLTDNLFVEKASGLTDAEVRQAVKTWLSVEDDPEVLEEEIAME
LQAVDEQLAGVTLDDDVPSEEEDDDGGLDDLAYHLSYLYDDTSYASSSTS
PSFTGGFHKDTIGGHGTSTAGSAAGAISSQSSLEEEACYGDELPACAGGC
ISASDVEAMLDDSTFDLDVFCPMYTCDGNTDIPSSECLSDDPVENLHQHG
GAAPGAQISVFDVSYVGTEVYASWAGNDLWYSAMETGAKIHTNSWGGATL
CQLTEKEYMYDMFMYENPEHLLIFSAGNDGGYEDIPNREACTVGSPALGK
NSLAVGATSSGPFPGTRTGDDGNLFYEEVGLTDYSPEGYPWICLMPYLGT
PSASTEPAGVDTVAYFSSQGPTLDGRIKPDVVAPGDQVASASSDGTDEHS
CKLAGPPLPAPWLPVPLPW
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Annotated Terms
The following terms have been associated with this polypeptide:
Vocabulary: INTERPRO
TermDefinition
IPR036852Peptidase_S8/S53_dom_sf
IPR000209Peptidase_S8/S53_dom