mRNA_Ecto-sp9_F_contig1045.430.1 (mRNA) Ectocarpus species9 EcSCA_722f female

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_Ecto-sp9_F_contig1045.430.1
Unique NamemRNA_Ecto-sp9_F_contig1045.430.1
TypemRNA
OrganismEctocarpus species9 EcSCA_722f female (Ectocarpus species9 EcSCA_722f female)
Homology
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BLAST of mRNA_Ecto-sp9_F_contig1045.430.1 vs.
Match: Ankyrin (repeat protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LRS0_ECTSI)

HSP 1 Score: 136 bits (343), Expect = 5.110e-36
Identity = 66/103 (64.08%), Postives = 80/103 (77.67%), Query Frame = 1 
Query:   28 MHLFPKRVPLQGLETDIFKLVSYGATPDQWANWLRVPLEHAAARGNLDLVNILIEAGANGSAGWRGCRGRTLLDTAALGXXXXXXXXXXXXGAKPDVTVVSMA 336
            M+++PKR  LQG+E++I+KLVSYGATP QW  WLRVPLEHAAARGNL+LVN L+EAGA+GSAGWRGC  RTL+D AALG            GA+PDV  VS++
Sbjct:    1 MYVYPKRRLLQGMESEIWKLVSYGATPQQWKEWLRVPLEHAAARGNLNLVNSLLEAGADGSAGWRGCLDRTLIDAAALGGSEPVVSALLRAGAQPDVNTVSIS 103
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BLAST of mRNA_Ecto-sp9_F_contig1045.430.1 vs.
Match: Uncharacterized (protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JW91_9PHAE)

HSP 1 Score: 129 bits (324), Expect = 1.730e-35
Identity = 64/107 (59.81%), Postives = 77/107 (71.96%), Query Frame = 1 
Query:   10 DYLISKMHLFPKRVPLQGLETDIFKLVSYGATPDQWANWLRVPLEHAAARGNLDLVNILIEAGANGSAGWRGCRGRTLLDTAALGXXXXXXXXXXXXGAKPDVTVVS 330
            D+  +K H++PKRV L+GLE +IFKLVSY ATP+QW  WL+VPLE AAA GN DL   L+EAGA GSAGWRGCR R+L+D AA+G            GA+PDV VVS
Sbjct:   38 DHTDTKTHIYPKRVLLEGLEIEIFKLVSYSATPEQWKEWLQVPLERAAALGNRDLFYKLLEAGAYGSAGWRGCRDRSLIDAAAVGGNVDVVSGFLEAGAEPDVQVVS 144
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BLAST of mRNA_Ecto-sp9_F_contig1045.430.1 vs.
Match: Ankyrin (repeat protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LRZ8_ECTSI)

HSP 1 Score: 134 bits (338), Expect = 2.760e-35
Identity = 64/103 (62.14%), Postives = 80/103 (77.67%), Query Frame = 1 
Query:   28 MHLFPKRVPLQGLETDIFKLVSYGATPDQWANWLRVPLEHAAARGNLDLVNILIEAGANGSAGWRGCRGRTLLDTAALGXXXXXXXXXXXXGAKPDVTVVSMA 336
            MH++P+R   QG+ ++I+KLVS+ ATP QW  WLRVPLEHAAARGNL+LVN L+EAGA+GSAGW+GCRGRTL+D AALG            GA+PDV VVS++
Sbjct:    1 MHVYPERRLFQGMGSEIWKLVSHSATPQQWKEWLRVPLEHAAARGNLNLVNSLLEAGADGSAGWKGCRGRTLIDAAALGGSEPVVSALLRAGAQPDVNVVSVS 103
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BLAST of mRNA_Ecto-sp9_F_contig1045.430.1 vs.
Match: Uncharacterized (protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5K1F4_9PHAE)

HSP 1 Score: 133 bits (334), Expect = 2.900e-34
Identity = 64/103 (62.14%), Postives = 74/103 (71.84%), Query Frame = 1 
Query:   28 MHLFPKRVPLQGLETDIFKLVSYGATPDQWANWLRVPLEHAAARGNLDLVNILIEAGANGSAGWRGCRGRTLLDTAALGXXXXXXXXXXXXGAKPDVTVVSMA 336
            MHL+PKR  L+G E +IFKL+ +GATP+QWA WLR PLEHAAARGNL LV  L+ AGANG AGWRGCRGRT+LD AALG            G   DV VVS++
Sbjct:    1 MHLYPKRALLEGAELEIFKLLYFGATPEQWAEWLRAPLEHAAARGNLGLVERLLNAGANGGAGWRGCRGRTMLDAAALGGNPDVVATLLASGCASDVNVVSLS 103
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BLAST of mRNA_Ecto-sp9_F_contig1045.430.1 vs.
Match: Ankyrin (repeat protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LS36_ECTSI)

HSP 1 Score: 132 bits (331), Expect = 3.870e-34
Identity = 64/103 (62.14%), Postives = 80/103 (77.67%), Query Frame = 1 
Query:   28 MHLFPKRVPLQGLETDIFKLVSYGATPDQWANWLRVPLEHAAARGNLDLVNILIEAGANGSAGWRGCRGRTLLDTAALGXXXXXXXXXXXXGAKPDVTVVSMA 336
            MH++P+R  LQG+E++I+KLVSY ATP QW  WLR+PLEHAAARGNL+LV+ L+ AGA+GSAGWRGCRGRTL+D AALG            GA+ DV VVS++
Sbjct:    1 MHVYPERRLLQGMESEIWKLVSYSATPQQWKEWLRMPLEHAAARGNLNLVSSLLGAGADGSAGWRGCRGRTLIDAAALGGSEPVVSTLLRAGAQWDVNVVSVS 103
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BLAST of mRNA_Ecto-sp9_F_contig1045.430.1 vs.
Match: Similar (to ankyrin 2,3/unc44, partial n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LE08_ECTSI)

HSP 1 Score: 127 bits (320), Expect = 9.840e-33
Identity = 72/103 (69.90%), Postives = 85/103 (82.52%), Query Frame = 1 
Query:   28 MHLFPKRVPLQGLETDIFKLVSYGATPDQWANWLRVPLEHAAARGNLDLVNILIEAGANGSAGWRGCRGRTLLDTAALGXXXXXXXXXXXXGAKPDVTVVSMA 336
            MHL+PKR  L+G E +IFKL+S+ ATP+QWA+WLR PLEHAAARGN+ LV  L+EAGA+G AGWRGCRGRT+LD A   XXXXXXXXXXX G  PDV VVS++
Sbjct:    1 MHLYPKRALLEGAEVEIFKLLSFCATPEQWADWLRAPLEHAAARGNIGLVERLLEAGADGRAGWRGCRGRTMLDAAXXXXXXXXXXXXXXSGCAPDVNVVSVS 103
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BLAST of mRNA_Ecto-sp9_F_contig1045.430.1 vs.
Match: Uncharacterized (protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5KNQ1_9PHAE)

HSP 1 Score: 124 bits (312), Expect = 1.590e-32
Identity = 60/103 (58.25%), Postives = 75/103 (72.82%), Query Frame = 1 
Query:   28 MHLFPKRVPLQGLETDIFKLVSYGATPDQWANWLRVPLEHAAARGNLDLVNILIEAGANGSAGWRGCRGRTLLDTAALGXXXXXXXXXXXXGAKPDVTVVSMA 336
            MH+FP+R  L GL +++FKLVSYGA+ +QWA WLRVPLEHAA+RGN +LV  L++AGA+  AGWRGCRGRTLLD AALG            GA  DV  V+++
Sbjct:    1 MHIFPERTSLDGLGSEVFKLVSYGASREQWAEWLRVPLEHAASRGNPNLVERLLQAGADAGAGWRGCRGRTLLDAAALGGNADVMSALIRGGAGADVNKVTVS 103
[Close]
BLAST of mRNA_Ecto-sp9_F_contig1045.430.1 vs.
Match: Uncharacterized (protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JY41_9PHAE)

HSP 1 Score: 122 bits (306), Expect = 1.950e-30
Identity = 60/103 (58.25%), Postives = 72/103 (69.90%), Query Frame = 1 
Query:   28 MHLFPKRVPLQGLETDIFKLVSYGATPDQWANWLRVPLEHAAARGNLDLVNILIEAGANGSAGWRGCRGRTLLDTAALGXXXXXXXXXXXXGAKPDVTVVSMA 336
            MHL PKR  L+  E +IFKL+S+GATP++WA WLR P EHAAARGN+ LV  L+EAGA+G AGWRGCR RT+LD AALG               PDV VVS++
Sbjct:    1 MHLDPKRALLEEAELEIFKLLSFGATPERWAEWLRAPQEHAAARGNIGLVERLLEAGADGGAGWRGCRSRTMLDAAALGGNADVVTKLLRSECVPDVNVVSVS 103
[Close]
BLAST of mRNA_Ecto-sp9_F_contig1045.430.1 vs.
Match: Similar (to ankyrin 2,3/unc44 n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LBP9_ECTSI)

HSP 1 Score: 119 bits (297), Expect = 3.790e-29
Identity = 58/101 (57.43%), Postives = 68/101 (67.33%), Query Frame = 1 
Query:   34 LFPKRVPLQGLETDIFKLVSYGATPDQWANWLRVPLEHAAARGNLDLVNILIEAGANGSAGWRGCRGRTLLDTAALGXXXXXXXXXXXXGAKPDVTVVSMA 336
            ++P+R  L+G E  IF L+SYGATP+QW  WLR PLEHAAARG   LV  L+ AGA+G AGW GCRGRTLLD AALG            G  PDV VVS++
Sbjct:    1 MYPRRPVLEGAEVGIFTLLSYGATPEQWREWLRAPLEHAAARGEFVLVEKLLNAGADGGAGWSGCRGRTLLDAAALGGNPDVVTTLLRAGCLPDVNVVSVS 101
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BLAST of mRNA_Ecto-sp9_F_contig1045.430.1 vs.
Match: Uncharacterized (protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FXA8_ECTSI)

HSP 1 Score: 113 bits (283), Expect = 3.150e-27
Identity = 55/90 (61.11%), Postives = 65/90 (72.22%), Query Frame = 1 
Query:   61 GLETDIFKLVSYGATPDQWANWLRVPLEHAAARGNLDLVNILIEAGANGSAGWRGCRGRTLLDTAALGXXXXXXXXXXXXGAKPDVTVVS 330
            G E +IFK+VS GATP+QWA WLR PLEHAAA GNL+LV+ L++AG +G AGWRGCRGR+LLD AALG            G  PDV VV+
Sbjct:   10 GEEVEIFKIVSEGATPEQWARWLRAPLEHAAASGNLNLVDRLLKAGVDGKAGWRGCRGRSLLDAAALGGNAGVMSALLQAGCGPDVNVVA 99
The following BLAST results are available for this feature:
BLAST of mRNA_Ecto-sp9_F_contig1045.430.1 vs.
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Ectocarpus species9 EcSCA_722f female vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
Ankyrin5.110e-3664.08repeat protein n=1 Tax=Ectocarpus siliculosus TaxI... [more]
Uncharacterized1.730e-3559.81protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=... [more]
Ankyrin2.760e-3562.14repeat protein n=1 Tax=Ectocarpus siliculosus TaxI... [more]
Uncharacterized2.900e-3462.14protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=... [more]
Ankyrin3.870e-3462.14repeat protein n=1 Tax=Ectocarpus siliculosus TaxI... [more]
Similar9.840e-3369.90to ankyrin 2,3/unc44, partial n=1 Tax=Ectocarpus s... [more]
Uncharacterized1.590e-3258.25protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=... [more]
Uncharacterized1.950e-3058.25protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=... [more]
Similar3.790e-2957.43to ankyrin 2,3/unc44 n=1 Tax=Ectocarpus siliculosu... [more]
Uncharacterized3.150e-2761.11protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 ... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
Ecto-sp9_F_contig1045contigEcto-sp9_F_contig1045:25385..25723 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Ectocarpus species9 EcSCA_722f female vs UniRef902022-09-19
OGS1.0 of Ectocarpus species9 EcSCA_722f female2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score137.9
Seed ortholog evalue2.7e-30
Seed eggNOG ortholog2880.D8LRS0
KEGG koko:K21440
GOsGO:0001508,GO:0002027,GO:0002262,GO:0002376,GO:0002520,GO:0003008,GO:0003012,GO:0003013,GO:0003015,GO:0003205,GO:0003230,GO:0003279,GO:0003283,GO:0003674,GO:0005198,GO:0005200,GO:0005488,GO:0005515,GO:0005575,GO:0005622,GO:0005623,GO:0005634,GO:0005737,GO:0005783,GO:0005829,GO:0005856,GO:0005886,GO:0005911,GO:0005938,GO:0006725,GO:0006778,GO:0006779,GO:0006807,GO:0006810,GO:0006811,GO:0006812,GO:0006816,GO:0006873,GO:0006874,GO:0006875,GO:0006887,GO:0006888,GO:0006928,GO:0006936,GO:0006937,GO:0006941,GO:0006942,GO:0006996,GO:0007009,GO:0007010,GO:0007154,GO:0007163,GO:0007165,GO:0007267,GO:0007275,GO:0007507,GO:0008015,GO:0008016,GO:0008092,GO:0008093,GO:0008104,GO:0008150,GO:0008152,GO:0009058,GO:0009893,GO:0009987,GO:0010256,GO:0010468,GO:0010522,GO:0010604,GO:0010628,GO:0010638,GO:0010646,GO:0010880,GO:0010881,GO:0010882,GO:0010959,GO:0012505,GO:0014704,GO:0014731,GO:0015672,GO:0016020,GO:0016043,GO:0016192,GO:0016323,GO:0016528,GO:0016529,GO:0018130,GO:0019222,GO:0019438,GO:0019722,GO:0019725,GO:0019899,GO:0019900,GO:0019901,GO:0019932,GO:0022898,GO:0023051,GO:0023052,GO:0030001,GO:0030003,GO:0030011,GO:0030016,GO:0030017,GO:0030018,GO:0030029,GO:0030048,GO:0030054,GO:0030097,GO:0030099,GO:0030154,GO:0030218,GO:0030315,GO:0030424,GO:0030507,GO:0030673,GO:0030674,GO:0030863,GO:0031252,GO:0031253,GO:0031256,GO:0031430,GO:0031647,GO:0031672,GO:0031674,GO:0032409,GO:0032411,GO:0032412,GO:0032414,GO:0032501,GO:0032502,GO:0032589,GO:0032879,GO:0032940,GO:0032970,GO:0033013,GO:0033014,GO:0033036,GO:0033043,GO:0033267,GO:0033292,GO:0033365,GO:0034101,GO:0034394,GO:0034613,GO:0034641,GO:0034762,GO:0034764,GO:0034765,GO:0034767,GO:0035088,GO:0035090,GO:0035556,GO:0035637,GO:0036309,GO:0036371,GO:0042383,GO:0042391,GO:0042592,GO:0042692,GO:0042995,GO:0043005,GO:0043034,GO:0043226,GO:0043227,GO:0043228,GO:0043229,GO:0043231,GO:0043232,GO:0043266,GO:0043268,GO:0043269,GO:0043270,GO:0043292,GO:0044057,GO:0044093,GO:0044237,GO:0044249,GO:0044271,GO:0044291,GO:0044304,GO:0044325,GO:0044422,GO:0044424,GO:0044425,GO:0044444,GO:0044448,GO:0044449,GO:0044456,GO:0044459,GO:0044463,GO:0044464,GO:0045197,GO:0045199,GO:0045202,GO:0045211,GO:0046483,GO:0046903,GO:0046907,GO:0048193,GO:0048468,GO:0048513,GO:0048518,GO:0048522,GO:0048534,GO:0048731,GO:0048821,GO:0048856,GO:0048869,GO:0048872,GO:0048878,GO:0050789,GO:0050794,GO:0050801,GO:0050821,GO:0050896,GO:0051049,GO:0051050,GO:0051117,GO:0051128,GO:0051130,GO:0051179,GO:0051186,GO:0051188,GO:0051234,GO:0051239,GO:0051270,GO:0051279,GO:0051282,GO:0051641,GO:0051649,GO:0051716,GO:0051899,GO:0051924,GO:0051928,GO:0055001,GO:0055065,GO:0055072,GO:0055074,GO:0055076,GO:0055080,GO:0055082,GO:0055117,GO:0060047,GO:0060048,GO:0060090,GO:0060255,GO:0060306,GO:0060307,GO:0060341,GO:0061024,GO:0061061,GO:0061245,GO:0061337,GO:0061515,GO:0065007,GO:0065008,GO:0065009,GO:0070252,GO:0070296,GO:0070727,GO:0070838,GO:0070972,GO:0071704,GO:0071840,GO:0071944,GO:0072359,GO:0072503,GO:0072507,GO:0072511,GO:0072657,GO:0072659,GO:0086001,GO:0086002,GO:0086003,GO:0086004,GO:0086005,GO:0086010,GO:0086012,GO:0086014,GO:0086015,GO:0086018,GO:0086019,GO:0086026,GO:0086046,GO:0086065,GO:0086066,GO:0086070,GO:0086091,GO:0090257,GO:0097060,GO:0097458,GO:0098590,GO:0098771,GO:0098794,GO:0098900,GO:0098901,GO:0098907,GO:0098910,GO:0099080,GO:0099081,GO:0099512,GO:0099568,GO:0099623,GO:0120025,GO:0120038,GO:1901016,GO:1901018,GO:1901019,GO:1901021,GO:1901360,GO:1901362,GO:1901379,GO:1901381,GO:1901564,GO:1901566,GO:1901576,GO:1903115,GO:1903169,GO:1903522,GO:1903779,GO:1904062,GO:1904064,GO:1904427,GO:1990778,GO:2001257,GO:2001259
EggNOG free text desc.response to abiotic stimulus
EggNOG OGsCOG0666@1,KOG4177@2759
COG Functional cat.G
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko04131
Hectar predicted targeting categoryother localisation
Exons1
Model size339
Cds size312
Stop0
Start1
Relationships

The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622928254.7611396-UTR-Ecto-sp9_F_contig1045:25384..254111622928254.7611396-UTR-Ecto-sp9_F_contig1045:25384..25411Ectocarpus species9 EcSCA_722f femaleUTREcto-sp9_F_contig1045 25385..25411 +
1692277810.6936505-UTR-Ecto-sp9_F_contig1045:25384..254111692277810.6936505-UTR-Ecto-sp9_F_contig1045:25384..25411Ectocarpus species9 EcSCA_722f femaleUTREcto-sp9_F_contig1045 25385..25411 +


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622928254.7738364-CDS-Ecto-sp9_F_contig1045:25411..257231622928254.7738364-CDS-Ecto-sp9_F_contig1045:25411..25723Ectocarpus species9 EcSCA_722f femaleCDSEcto-sp9_F_contig1045 25412..25723 +
1692277810.705127-CDS-Ecto-sp9_F_contig1045:25411..257231692277810.705127-CDS-Ecto-sp9_F_contig1045:25411..25723Ectocarpus species9 EcSCA_722f femaleCDSEcto-sp9_F_contig1045 25412..25723 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_Ecto-sp9_F_contig1045.430.1prot_Ecto-sp9_F_contig1045.430.1Ectocarpus species9 EcSCA_722f femalepolypeptideEcto-sp9_F_contig1045 25412..25723 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_Ecto-sp9_F_contig1045.430.1

>prot_Ecto-sp9_F_contig1045.430.1 ID=prot_Ecto-sp9_F_contig1045.430.1|Name=mRNA_Ecto-sp9_F_contig1045.430.1|organism=Ectocarpus species9 EcSCA_722f female|type=polypeptide|length=104bp
MHLFPKRVPLQGLETDIFKLVSYGATPDQWANWLRVPLEHAAARGNLDLV
NILIEAGANGSAGWRGCRGRTLLDTAALGGSADVVTALLRAGAKPDVTVV
SMAP
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mRNA from alignment at Ecto-sp9_F_contig1045:25385..25723+

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_Ecto-sp9_F_contig1045.430.1 ID=mRNA_Ecto-sp9_F_contig1045.430.1|Name=mRNA_Ecto-sp9_F_contig1045.430.1|organism=Ectocarpus species9 EcSCA_722f female|type=mRNA|length=339bp|location=Sequence derived from alignment at Ecto-sp9_F_contig1045:25385..25723+ (Ectocarpus species9 EcSCA_722f female)
CACCACGACGACTACCTCATTAGCAAGATGCACCTCTTTCCCAAGCGAGT
ACCCCTGCAAGGCCTGGAGACCGATATCTTCAAGCTCGTTTCGTACGGCG
CCACGCCCGACCAGTGGGCAAACTGGCTTCGGGTGCCTCTCGAACACGCC
GCTGCCCGCGGCAACCTGGATCTGGTGAACATCCTGATCGAAGCTGGGGC
GAACGGGAGTGCGGGATGGAGAGGGTGTCGAGGTCGCACTCTGTTGGACA
CGGCCGCGCTCGGCGGCAGCGCAGACGTGGTCACCGCCTTGCTTCGGGCG
GGGGCGAAACCGGACGTGACTGTTGTGTCCATGGCCCCG
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Coding sequence (CDS) from alignment at Ecto-sp9_F_contig1045:25385..25723+

>mRNA_Ecto-sp9_F_contig1045.430.1 ID=mRNA_Ecto-sp9_F_contig1045.430.1|Name=mRNA_Ecto-sp9_F_contig1045.430.1|organism=Ectocarpus species9 EcSCA_722f female|type=CDS|length=624bp|location=Sequence derived from alignment at Ecto-sp9_F_contig1045:25385..25723+ (Ectocarpus species9 EcSCA_722f female)
ATGCACCTCTTTCCCAAGCGAGTACCCCTGCAAGGCCTGGAGACCGATAT
CTTCAAGCTCGTTTCGTACGGCGCCACGCCCGACCAGTGGGCAAACTGGC
TTCGGGTGCCTCTCGAACACGCCGCTGCCCGCGGCAACCTGGATCTGGTG
AACATCCTGATCGAAGCTGGGGCGAACGGGAGTGCGGGATGGAGAGGGTG
TCGAGGTCGCACTCTGTTGGACACGGCCGCGCTCGGCGGCAGCGCAGACG
TGGTCACCGCCTTGCTTCGGGCGGGGGCGAAACCGGACGTGACTGTTGTG
TCCATGGCCCCGATGCACCTCTTTCCCAAGCGAGTACCCCTGCAAGGCCT
GGAGACCGATATCTTCAAGCTCGTTTCGTACGGCGCCACGCCCGACCAGT
GGGCAAACTGGCTTCGGGTGCCTCTCGAACACGCCGCTGCCCGCGGCAAC
CTGGATCTGGTGAACATCCTGATCGAAGCTGGGGCGAACGGGAGTGCGGG
ATGGAGAGGGTGTCGAGGTCGCACTCTGTTGGACACGGCCGCGCTCGGCG
GCAGCGCAGACGTGGTCACCGCCTTGCTTCGGGCGGGGGCGAAACCGGAC
GTGACTGTTGTGTCCATGGCCCCG
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