mRNA_Ecto-sp9_F_contig1068.606.1 (mRNA) Ectocarpus species9 EcSCA_722f female

BLAST of mRNA_Ecto-sp9_F_contig1068.606.1 vs.
Match: Small (Conductance Mechanosensitive Ion channel n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FX37_ECTSI)

HSP 1 Score: 1758 bits (4552), Expect = 0.000e+0
Identity = 966/1050 (92.00%), Postives = 976/1050 (92.95%), Query Frame = 1

Query:    1 RQMSRMDMFSGTKKSGGAGTSSRPGHRKTSSGMGFLLSSAQPHSRVPSRTDDRSPWEAASIAAAAPPPLPRPTGAGVAQTPVAVTSTTAGQQQRGSQVQPDSNVSPGRLERATGGGGGESGVDGCRIASKTPAGVAVAHGVGHSGGAGASRPPPQDDVNYPDTPPPLRRMQRRGSLVNREVPQEDEPKEPIEPELMIGEIQSIRLHEEIALVRRAIVLIMLSLVAAVLVRAFAGELDKDVNIREVVFFVSLLLLSFPVIRFTARLFAFVITTRASGLLYRGNRYYFVVALHYDIAVTIWSVVAMYIWKELFHEWVYTDDSKLARAIFRHVSPVLECHLALRVGILLKNYLVLLVATSYLWRPYLQRVQSSILAQYILLLLTDYCTKGYCNPGDQNFAIEMGRQGIKEGKNISLYAVSKAMGFIPRNKLGHTFFRQLAGSNTLDSSKDATTLGNFLFDQLIVRSRPPPARPXXXXXXXXXXXXXXXXXXXXGSIRMSRGSHGGAGTYRALPRFGGQMQKRNSEPMAVPASPRTPVASPKRFSMFEAGRPQVEGMPLANGGGGGAAAGKVPVAKAVSSKASGTLSKLVPVAGRAALVSSGRGGAGGGTESSDVRGDDAGTTSSANTSPGRSRVRRRKAGTCVDGVDLSIEVASGGETEATGDSGTEWESPEQPVPSMSPSGRDTRIDRARILAQNRSVFTSCSSVASSSNYGDKECGDGVDGEGRQKDQEDRVKGQAARSGETKAEKKEKPKQEEVLVRKTFCPALEPELLDVAFKIFDLDNSGTVTKEEMVLGVVGTFKDHRSLAHTLQDSEHIAQKLGLIIMCVILFILFFVWLSIWGADVVSLSVTFASFLIAFSFMIGTAASNLMSAVLFIFVSRLYDVGDRVHIYSGSNTQGEEPTNVTVVKVDLMTTVFKRWDEQVFYMPNHLLATKTIVNIQRTAHQWHEFMIQVAATTTPEKLTALQTSLQEFSKSKDKPEGLYTRMGFSLVRIEDSTKLTIRITFRQRGNWQNMEKKWACQSMCTWAIKSACDSLNISYFLPEVPVQMKKKSA 3150
            +QMSRMDMFSGTKKSGGAGT+SRPGH+K SSGMGFLLSSAQPHSRVPSRTDDRSPWE ASIA AAPPPLPRPTGAGVAQTPVAVTSTTAG+QQRG+QVQPD NVSPGRLERATGGGGGE GVDGCR ASKTPAGVAV HGVGHSGGAGASRPPPQDDVNYPDTPPPLRRMQRRGSLVNREVPQEDEPKEPIEPELMIGEIQSIRLHEEIALVRRAIVLIMLSLVAAVLVRAFAGE                                                YYFVVALHYDIAVTIWSVVAMYIW+ELFH+WVYTDDSKLARAIFRHV+P LECHLALRVGILLKNYLVLLVATSYLWRPYLQRVQSSILAQYILLLLTDYCTKGYCNPGDQNFAIEMGRQGIKEGKNISLYAVSKAMGFIPRNKLGHTFFRQLAGSNTLDSSKDATTLGNFLFDQLI+RSRPPPA  XXXXXXXXXXXXXXXXXXXXGSIRMSRGSH GAGTYRALPRFGGQMQKRNSEPMAVPASPRT VASPKRFSMFEAGRPQVEGMPLANGG     AGKVPVAKAVSSKAS TLSKLVPVAGRAALVSSGRGGAGGGTESSD R DD GTTSSANTSPGRSRVRRRKAGTCVDGVDLSIEVASGGETEATGD+GTE ESPEQPV S SPSGRDTRIDRARILAQNRSVFTSCSSVASSSNYGDKECGDGVDGEGRQKDQED VKGQAARSGETKAEKKEKPKQEEVLVR TFCPALEPELLDVAFKIFDLDNSGTVTKEEMVLGVVGTFKDHRSLAHTLQDSEHIAQKLGLIIMCVILFILFFVWLSIWGADVVSLSVTFASFLIAFSFMIGTAASNLMSAVLFIFVSRLYDVGDRVHIYSGSNTQGEEPTNVTVVKVDLMTTVFKRWDEQVFYMPNHLLATKTIVNIQRTAHQWHEFMIQVAATTTPEKLTALQTSLQEFSKSKDKPEGLYTRMGFSLVRIEDSTKLTIRITFRQRGNWQNMEKKWACQSMCTWAIKSACDSLNISYFLPEVPVQMKKKSA
Sbjct:  131 KQMSRMDMFSGTKKSGGAGTNSRPGHKKNSSGMGFLLSSAQPHSRVPSRTDDRSPWEVASIATAAPPPLPRPTGAGVAQTPVAVTSTTAGRQQRGNQVQPDGNVSPGRLERATGGGGGEYGVDGCRNASKTPAGVAVTHGVGHSGGAGASRPPPQDDVNYPDTPPPLRRMQRRGSLVNREVPQEDEPKEPIEPELMIGEIQSIRLHEEIALVRRAIVLIMLSLVAAVLVRAFAGEF-----------------------------------------------YYFVVALHYDIAVTIWSVVAMYIWRELFHQWVYTDDSKLARAIFRHVNPALECHLALRVGILLKNYLVLLVATSYLWRPYLQRVQSSILAQYILLLLTDYCTKGYCNPGDQNFAIEMGRQGIKEGKNISLYAVSKAMGFIPRNKLGHTFFRQLAGSNTLDSSKDATTLGNFLFDQLIIRSRPPPAXXXXXXXXXXXXXXXXXXXXXXGSIRMSRGSHAGAGTYRALPRFGGQMQKRNSEPMAVPASPRTTVASPKRFSMFEAGRPQVEGMPLANGGXXXXXAGKVPVAKAVSSKASSTLSKLVPVAGRAALVSSGRGGAGGGTESSDARCDDVGTTSSANTSPGRSRVRRRKAGTCVDGVDLSIEVASGGETEATGDTGTEGESPEQPVASKSPSGRDTRIDRARILAQNRSVFTSCSSVASSSNYGDKECGDGVDGEGRQKDQEDTVKGQAARSGETKAEKKEKPKQEEVLVRTTFCPALEPELLDVAFKIFDLDNSGTVTKEEMVLGVVGTFKDHRSLAHTLQDSEHIAQKLGLIIMCVILFILFFVWLSIWGADVVSLSVTFASFLIAFSFMIGTAASNLMSAVLFIFVSRLYDVGDRVHIYSGSNTQGEEPTNVTVVKVDLMTTVFKRWDEQVFYMPNHLLATKTIVNIQRTAHQWHEFMIQVAATTTPEKLTALQTSLQEFSKSKDKPEGLYTRMGFSLVRIEDSTKLTIRITFRQRGNWQNMEKKWACQSMCTWAIKSACDSLNISYFLPEVPVQMKKKSA 1133          

BLAST of mRNA_Ecto-sp9_F_contig1068.606.1 vs.
Match: EF-hand (domain-containing protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JDS1_9PHAE)

HSP 1 Score: 1666 bits (4315), Expect = 0.000e+0
Identity = 910/1059 (85.93%), Postives = 936/1059 (88.39%), Query Frame = 1

Query:    1 RQMSRMDMFSGTKKSGGAGTSSRPGHRKTSSGMGFLLSSAQPHSRVPSRTDDRSPWEAASIAAAAPPPLPRPTGAGVAQTPVAVTSTTAGQQQRGSQVQPDSNVSPGRLERATGGGGGESGVDGCRIASKTPAGVAVAHGVGHSGGAGASRPPPQDDVNYPDTPPPLRRMQRRGSLVNREVPQEDEPKEPIEPELMIGEIQSIRLHEEIALVRRAIVLIMLSLVAAVLVRAFAGELDKDVNIREVVFFVSLLLLSFPVIRFTARLFAFVITTRASGLLYRGNRYYFVVALHYDIAVTIWSVVAMYIWKELFHEWVYTDDSKLARAIFRHVSPVLECHLALRVGILLKNYLVLLVATSYLWRPYLQRVQSSILAQYILLLLTDYCTKGYCNPGDQNFAIEMGRQGIKEGKNISLYAVSKAMGFIPRNKLGHTFFRQLAGSNTLDSSKDATTLGNFLFDQLIVRSRPPPARPXXXXXXXXXXXXXXXXXXXXGSIRMSRGSHGGAGTYRALPRFGGQMQKRNSEPMAVPASPRTPVASPKRFSMFEAGRPQVEGMPLANGGGGGAAAGKVPVAKAVSSKASGTLSKLVPVAGRAALVSSGRGGAGGGTESSDVRGDDAGTTSSANTSPGRSRVRRRKAGTCVDGVDLSIEVASGGETEATGDSGTEWESPEQPVPSMSPSGRDTRIDRARILAQNRSVFTSCSSVASSSNYGDKECGDGVDGEGRQKDQEDRVKGQAARSGETKAEKKEKPKQEEVLVRKTFCPALEPELLDVAFKIFDLDNSGTVTKEEMVLGVVGTFKDHRSLAHTLQDSEHIAQKLGLIIMCVILFILFFVWLSIWGADVVSLSVTFASFLIAFSFMIGTAASNLMSAVLFIFVSRLYDVGDRVHIYSGSNTQGEEPTNVTVVKVDLMTTVFKRWDEQVFYMPNHLLATKTIVNIQRTAHQWHEFMIQVAATTTPEKLTALQTSLQEFSKSKDKPEGLYTRMGFSLVRIEDSTKLTIRITFRQRGNWQ---------NMEKKWACQSMCTWAIKSACDSLNISYFLPEVPVQMKKKSA 3150
            +QMSRMDMFSGTKKSGGAGT+S PGHRKTSSGMGFLLSSAQPHSRVPSRTDDRSPWEAAS+AA APPPLPRPTGAGVAQTPVA TST AGQQ+RGSQ QP SN+S GRLERATGGGGG SGVDGCRIASKTPAGVAVAHGVGHS GAG S+PPPQ DVNYPDTPPPLRRMQRRGSLVNREVP ED+ KEPIEPELMIGEIQSIRLHEEIALVRRAIVLIMLSLVAAVLVR FAGELDKDVNIREVVFFVSLLLLSFPVIRFTARLFAFVITTRASGLLYRGNRYYFVVALHYDIAVTIWSVVA YIWKELFHEWVYTDDSK+AR IFRHVSPVLECHLALRVGILLKNYLVLLVATSYLWRPYLQRVQSSILAQYILLLLTDYCTKGYCNPGDQNFAIEMGRQGIKEGKNISLYAVSKAMGFIPRNKLGHTFFRQLAGSNTLDSSKDATTLGNFLFDQLI+RSRPPPARP                    GSIRMSRGSHG  GTYRALPRFGGQMQKRNSE MAVPASPRTPVASPKRFS F+AGRPQVEGMPLA GGGGGAAAGKVPVAKAVSSKAS TLSKLVPVAGRA+L+SSGRG A GGTESSDV G DAGTTSSANTSPGRSR RRR+A TCV+GV LSI   SGGE EATG++GTE ESPEQPV S SPSGRDTRIDRAR+LAQNR+VF+SCSSVA+SSNYGDKECGDGVDGEGRQ+DQE            T+AEKKEKPKQEEVLVRKTFCPALEPELLDVAFKIFDLDNSGT+TKEEMVLGVVGTFKDHRSLAHTLQDSEHIAQKLGLIIMCVILFILFFVWLSIWGADVVSLSVTFASFLIAFSFMIGTAASNLMSAVLFIFVSRLYDVGDRVHIYSGSNT+GEEPTNVTVVKVDLMTTVFKRWDEQ                                             SLQEFSKSKDKPEGLY RMGFSLVRIEDSTKLTIRITFRQRGNWQ         NMEKKWACQSMCTWAIKSACDSLNISYFLPE+PVQMK+KSA
Sbjct:  132 KQMSRMDMFSGTKKSGGAGTNSWPGHRKTSSGMGFLLSSAQPHSRVPSRTDDRSPWEAASMAAPAPPPLPRPTGAGVAQTPVAATSTRAGQQRRGSQGQPASNISAGRLERATGGGGGNSGVDGCRIASKTPAGVAVAHGVGHSVGAGDSQPPPQGDVNYPDTPPPLRRMQRRGSLVNREVPPEDDLKEPIEPELMIGEIQSIRLHEEIALVRRAIVLIMLSLVAAVLVREFAGELDKDVNIREVVFFVSLLLLSFPVIRFTARLFAFVITTRASGLLYRGNRYYFVVALHYDIAVTIWSVVARYIWKELFHEWVYTDDSKMARTIFRHVSPVLECHLALRVGILLKNYLVLLVATSYLWRPYLQRVQSSILAQYILLLLTDYCTKGYCNPGDQNFAIEMGRQGIKEGKNISLYAVSKAMGFIPRNKLGHTFFRQLAGSNTLDSSKDATTLGNFLFDQLIIRSRPPPARPTTKTSPPRKSLAAAFTGAGGGSIRMSRGSHGVGGTYRALPRFGGQMQKRNSESMAVPASPRTPVASPKRFSTFDAGRPQVEGMPLAIGGGGGAAAGKVPVAKAVSSKASSTLSKLVPVAGRASLMSSGRGSASGGTESSDVPGGDAGTTSSANTSPGRSRGRRREARTCVNGVGLSIADGSGGEMEATGETGTEGESPEQPVVSTSPSGRDTRIDRARVLAQNRNVFSSCSSVANSSNYGDKECGDGVDGEGRQQDQEXXXXXXXXXXXXTEAEKKEKPKQEEVLVRKTFCPALEPELLDVAFKIFDLDNSGTITKEEMVLGVVGTFKDHRSLAHTLQDSEHIAQKLGLIIMCVILFILFFVWLSIWGADVVSLSVTFASFLIAFSFMIGTAASNLMSAVLFIFVSRLYDVGDRVHIYSGSNTRGEEPTNVTVVKVDLMTTVFKRWDEQ--------------------------------------------ASLQEFSKSKDKPEGLYPRMGFSLVRIEDSTKLTIRITFRQRGNWQVGQEAIVVVNMEKKWACQSMCTWAIKSACDSLNISYFLPEIPVQMKEKSA 1146          

BLAST of mRNA_Ecto-sp9_F_contig1068.606.1 vs.
Match: EF-hand (domain-containing protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JBC1_9PHAE)

HSP 1 Score: 818 bits (2113), Expect = 5.090e-270
Identity = 541/1086 (49.82%), Postives = 672/1086 (61.88%), Query Frame = 1

Query:    1 RQMSRMDMFSGTKKSGGAGTSSR---------PGHRKT-SSGMGFLLSSAQPHSRVPSRTDDRSPWEAASIAAAAPPPLPRPTGAGVAQTPVAVTSTTAGQQQRGSQVQPDSNVSPGRLERATGGGGGESGVDGCRIASKTPAGVAVAHGVGHSGGAGASRPPPQDDVNYPDTPPPLRRMQRRGSLVNREVPQEDEPKEPIEPELMIGEIQSIRLHEEIALVRRAIVLIMLSLVAAVLVRAFAGELDKDVNIREVVFFVSLLLLSFPVIRFTARLFAFVITTRASGLLYRGNRYYFVVALHYDIAVTIWSVVAMYIWKELFHEWVYTDDSKLARAIFRHVSPVLECHLALRVGILLKNYLVLLVATSYLWRPYLQRVQSSILAQYILLLLTDYCTKGYCNPGDQNFAIEMGRQGIKEGKNISLYAVSKAMGFIPRNKLGHTFFRQLAGSNTLDSSKDATTLGNFLFDQLIVRSRP------PPARPXXXXXXXXXXXXXXXXXXXXG-----SIRMSRGSHGGAGTYRALPRFGGQMQKRNSEPMAVPASPRTPVASPKRFSMFEAGRPQVEGMPLANGGGGGAAAGKVPVAKAVSSKASGTLSKLVPVAGRAALVSSGRGGAGGGTESSDVRGDDAGTTSSANTSP-------GRSRVRR-----RKAGTCVDGVD--LSIEVASGGETEATGD-SGTEWESPE-QPVPSMSPSGRDTRIDRARILAQNRSVFTSCSSVASSSNYGDKECG-DGVDGEGRQKDQEDRVKGQAARSGETKAEKKEKPKQEEVLVRKTFCPALEPELLDVAFKIFDLDNSGTVTKEEMVLGVVGTFKDHRSLAHTLQDSEHIAQKLGLIIMCVILFILFFVWLSIWGADVVSLSVTFASFLIAFSFMIGTAASNLMSAVLFIFVSRLYDVGDRVHIYSGSNTQGEEPTNVTVVKVDLMTTVFKRWDEQVFYMPNHLLATKTIVNIQRTAHQWHEFMIQVAATTTPEKLTALQTSLQEFSKSKDKPEGLYTRMGFSLVRIEDSTKLTIRITFRQRGNWQNMEKKWACQSMCTWAIKSACDSLNISYFLPEVPVQMKKK 3144
            R +S  D+FS     G  GTS R         P H+K  SS MGFL  S       PS T+ R P    S++AA           G +    A     A    R   +   ++V+P  LE     GG   GVD  R   + PA    A  +GH      S P   D V  P TPP L   ++R +LV+    +E+      E +LMIGEIQSI++ +EI L R+A++LI+ SLV A L R FAG LDKDVNI  VV F S + LS+P+IRFT RLFA+ +TTRAS LLYRGN +Y+VVALH DI++TIWSV +M++W++LF  WVY  DS+L+   +R+   + +C++A RVG+LLKN+LVLLVATSYLWRPYLQRVQSSILAQYILLLLTDY   G CNP DQ FAIEMGR+GIKE + ISLYAVSKAMGFIPR+KLGH FFR+L GSNTL+SS+DA TLG ++FDQL++RSR       PPA P                          S R  RG   G     +LP         +  P    +S  T    P+  S   A        P   GGG        P+    SSK S ++ K+       A   S  G A    +++D    DAG++              G +R R      ++A + +  VD     E+   GE++A G  SG E E P  + V  M+P       + A+ +A   S     S   + ++ GD   G D  DG  +++ +E  V+G  +      +++ +KP++EE+L R+T CP LE ++L++AF IFD+DN+G++TK EMVLGVV TFKDHRSLAHTL DSEHIA+KLGLII  +ILFIL FVWLSI G DV+SLSVTFASFLIAFSFMIGTAASNLM+AVLFIFVSRLYDVGDRVHIY    T G EP NV V KVDL TT F+RWDEQ+FY+PNHLLA KTIVNIQRTAHQWHEF I VAATT+  KL  L  +LQ+F+K KDKPEGLY RMGFSL  IEDST+L+IRI FRQRGNWQ+MEKKWACQSMCTWAIK+ACD++ I+Y+LPEVPV MK K
Sbjct:  122 RALSNGDLFS----HGAKGTSGREPSKTTSVGPSHKKNVSSLMGFLWPS-------PSSTEKRPPSGQTSLSAA--------DVGGASPFSGATKDYPASASGREQTLNYQASVNPHHLEE----GGVGGGVDETR---RHPA----AGSMGHVSDR-VSLPNALDGVRIPATPPQLDATEQRPNLVHVRETKEERIDTRSETDLMIGEIQSIQIADEIVLARKALMLILGSLVVATLFREFAGRLDKDVNIVNVVGFASTIFLSYPMIRFTIRLFAYFLTTRASALLYRGNSFYYVVALHQDISMTIWSVASMFVWRQLFAGWVYPTDSELSGKTYRYGDELFQCYVAYRVGMLLKNFLVLLVATSYLWRPYLQRVQSSILAQYILLLLTDYAATGKCNPDDQKFAIEMGRRGIKEQEKISLYAVSKAMGFIPRHKLGHAFFRELGGSNTLNSSRDAKTLGEYMFDQLLLRSRTYSPGCTPPASPSTKRWAYLANVGQTVLGGGSNANTLMSARSFRGGRLGPSNRASLPSSWTASDVGSLSPAKGTSSDATLKPPPRTRSRSGAEERAAGSAPREAGGGS-------PIVTRGSSKLSSSVDKMRMGPNARAASDSAAGAAAVTVDTADA--SDAGSSERPRNEVLPSEGGGGMARFREFHGGGKRALSVIQDVDSFADSELFVAGESKAEGTASGGEMEFPSLRVVDGMAPRHGHDVAELAKEIANGGSFS---SQDRNDNDAGDASRGKDSADGTVQKEHEESIVQGNGSTQKGGSSDEVDKPQEEELLTRETLCPMLEEQVLEMAFTIFDMDNNGSITKAEMVLGVVDTFKDHRSLAHTLHDSEHIARKLGLIIFWLILFILAFVWLSILGFDVLSLSVTFASFLIAFSFMIGTAASNLMTAVLFIFVSRLYDVGDRVHIYDDVQTAGVEPMNVVVTKVDLRTTSFRRWDEQIFYIPNHLLAEKTIVNIQRTAHQWHEFYIHVAATTSSRKLETLHNALQQFAKKKDKPEGLYPRMGFSLTGIEDSTRLSIRIVFRQRGNWQSMEKKWACQSMCTWAIKNACDTIGITYYLPEVPVVMKNK 1164          

BLAST of mRNA_Ecto-sp9_F_contig1068.606.1 vs.
Match: Small (Conductance Mechanosensitive Ion channel n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FX36_ECTSI)

HSP 1 Score: 574 bits (1480), Expect = 6.030e-179
Identity = 418/985 (42.44%), Postives = 536/985 (54.42%), Query Frame = 1

Query:  310 VSPGRLERATGGGGGESGVDGCRIASKTPAGVAVAHGVGHSGGAGASRPPPQDDVNYPDTPPPLRRMQRRGSLVNREVPQEDEPKEPIEPELMIGEIQSIRLHEEIALVRRAIVLIMLSLVAAVLVRAFAGELDKDVNIREVVFFVSLLLLSFPVIRFTARLFAFVITTRASGLLYRGNRYYFVVALHYDIAVTIWSVVAMYIWKELFHEWVYTDDSKLARAIFRHVSPVLECHLALRVGILLKNYLVLLVATSYLWRPYLQRVQSSILAQYILLLLTDYCTKGYCNPGDQNFAIEMGRQGIKEGKNISLYAVSKAMGFIPRNKLGHTFFRQLAGSNTLDSSKDATTLGNFLFDQLIVRSR------PPPARPXXXXXXXXXXXXXXXXXXXXGSIRMSRGSHGGAGTYRALPRFGGQM--QKRNSEPMAVPASPRTPVASPKRFSMFEAGRPQVEGMPLANGGGGGAAA-------GKVPVAKAVSSKASGTLSKLVPVAGRAALVSSGRGGAGGGTESSDVRGDDAGTTS-------SANTSPGRSRVRR-----RKAGTCVDGVD--LSIEVASGGETEA-TGDSGTEWE-SPEQPVPSMSPSGRDTRIDRARILAQNRSVFTSCSSVASSSNYGDKECGDGVDGEGR-----QKDQEDRVKGQAARSGETK----AEKKEKPKQEEVLVRKTFCPALEPELLDVAFKIFDLDNSGTVTKEEMVLGVVGTFKDHRSLAHTLQDSEHIAQKLGLIIMCVILFILFFVWLSIWGADVVSLSVTFASFLIAFSFMIGTAASNLMSAVLFIFVSRLYDVGDRVHIYSGSNTQGEEPTNVTVVKVDLMTTVFKRWDEQVFYMPNHLLATKTIVNIQRTAHQWHEFMIQVAATTTPEKLTALQTSLQEFSKSKDKPEGLYTRMGFSLVRIEDSTKLTIRITFRQRGNWQNMEKKWACQSMCTWAIKSACDSLNISYFLPEVPVQMKKK 3144
            V+P  LE    GGG +        AS+ PA  ++ H  G       S P   D V  P TPP L   + R +LV+    +E+      E ELMIGEI+SI++ +EI L R+A+VLI +SLV A L R FAG                                                 +YFVVALH DI++TIWS+ +M++W+ LF EWVY  DS+L+   +R+                                      QSSILAQYILLLLTDY T G CNP DQ FAIEMGR+GIKE + ISLYAVSKAMGFIPR+KLGH FFR+L G+NTL+SS+DA TLG ++FDQL++RSR        PA P                    G   +  G    A T  A    GG++   KR S P +  A     ++  KR S     +P    +   +G GG AA        G+ P+   VSSK S +++K+       A   S  G A    +++D    DAG++         +    G +  R      ++A + ++  D     +   GGE++A    S  E E S  + V +M+P       + A+  A   S         SS +  DK+ G    GEG      QK+ E+    Q+  +G T+    +++ +KP++EE+L R+T CP LE ++L++AFKIFDL+++G++TK                                            FVWLSI G DV+SLSVTFASFLIAFSFMIGTAASNLMSAVLFIFVSRLYDVGDRVHIY    T G EP NV VVKVDL TT F+RWDEQ+FY+PNHLLA KTIVNIQRTAHQWHEF I VAATT+ +KL  L  +LQ+F+K KDKPEGL+ RMGFSL  IEDST+L+IRI FRQRGNWQNM+KKWACQSMCTWAIK+ACD++ I+Y+LPEVP+ MK K
Sbjct:  196 VNPHHLEEGGIGGGVDK-------ASRHPATGSMGHVSGRM-----SLPNALDGVRIPATPPQLNATENRPNLVHVHDTKEERMDTRSETELMIGEIKSIQITDEIVLARKALVLIFVSLVGATLFREFAGPF-----------------------------------------------FYFVVALHQDISMTIWSIASMFVWRRLFAEWVYPTDSELSGNAYRYGDEFF---------------------------------QSSILAQYILLLLTDYATTGECNPDDQKFAIEMGRRGIKEQEKISLYAVSKAMGFIPRHKLGHAFFRELGGTNTLNSSRDAKTLGEYMFDQLLLRSRMYSPGCTAPASPATRRWAYLANV---------GQTVLGGGGGSNANTLNAKSFRGGRLGASKRASLPSSWTAGDVGSLSPSKRTSSDATLKP-APWLRSRSGAGGRAAGSAPREAGGRSPIVTRVSSKLSSSVNKMRMGPNARAASDSAAGAATATVDTADA--SDAGSSEIPMDEVLPSQGGGGMAGFREFHGGGKRALSVIEDADSFADSDPFVGGESKAEVTASCAEMEFSSLRVVDAMAPRYGHDVSELAKETANGGSF--------SSEDRNDKDAGGASRGEGSAGGTVQKEHEESTNVQS--NGSTQKGGDSDEADKPQEEELLTRETLCPMLEHQVLEMAFKIFDLNSNGSITKA-------------------------------------------FVWLSILGFDVLSLSVTFASFLIAFSFMIGTAASNLMSAVLFIFVSRLYDVGDRVHIYDDVQTAGVEPMNVVVVKVDLRTTSFRRWDEQIFYIPNHLLADKTIVNIQRTAHQWHEFYIHVAATTSSQKLETLHDALQKFAKKKDKPEGLHPRMGFSLTGIEDSTRLSIRIIFRQRGNWQNMDKKWACQSMCTWAIKNACDTIGITYYLPEVPIVMKNK 1023          

BLAST of mRNA_Ecto-sp9_F_contig1068.606.1 vs.
Match: Mechanosensitive (ion channel-domain-containing protein n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835YNP7_9STRA)

HSP 1 Score: 267 bits (682), Expect = 4.620e-77
Identity = 130/280 (46.43%), Postives = 194/280 (69.29%), Query Frame = 1

Query: 2305 LDVAFKIFDLDNSGTVTKEEMVLGVVGTFKDHRSLAHTLQDSEHIAQKLGLIIMCVILFILFFVWLSIWGADVVSLSVTFASFLIAFSFMIGTAASNLMSAVLFIFVSRLYDVGDRVHIYSGSNTQGEEPTNVTVVKVDLMTTVFKRWDEQVFYMPNHLLATKTIVNIQRTAHQWHEFMIQVAATTTPEKLTALQTSLQEFSKSKDKPEGLYTRMGFSLVRIEDSTKLTIRITFRQRGNWQNMEKKWACQSMCTWAIKSACDSLNISYFLPEVPVQMKKK 3144
            ++  F +FD D+S  +T EE+V GVV  +KDH+SLA TLQDS+ +A KLG ++   IL  L F+WL I+G D+ +L++TFAS L+  S +IG A++NL ++++FIFV R +DVGDRV  +        E  N  V ++DL+ T F+ WDE+V+Y+PNH+LAT+ I+NIQR+  QWHEF IQV A+T  +KL  L+    EF++  +KP G++  + F+L  +E++ +LTIR+ FRQ+GNWQ + +KWA QSMCT  I ++C  L I+Y  P++P+   K+
Sbjct:    1 MEQVFALFDRDDSNDITHEEVVNGVVNIYKDHQSLALTLQDSQRMAHKLGEVLGAGILIALIFIWLRIFGRDIFALTLTFASVLVGVSVLIGPASANLFASIVFIFVQRPFDVGDRVFHWFPDGRG--EMQNAVVTRIDLLITCFRAWDEKVYYLPNHVLATRPIINIQRSGDQWHEFFIQVLASTPSQKLWELKAKCVEFAEKHNKPSGIHPHIQFALTGVENTNRLTIRVCFRQKGNWQILSRKWAVQSMCTNCIVNSCKELGITYTQPQLPLDFNKQ 278          

BLAST of mRNA_Ecto-sp9_F_contig1068.606.1 vs.
Match: Mechanosensitive (ion channel-domain-containing protein n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835Z0M4_9STRA)

HSP 1 Score: 264 bits (675), Expect = 3.480e-69
Identity = 238/816 (29.17%), Postives = 370/816 (45.34%), Query Frame = 1

Query:  769 PVIRFTARLFAFVITTRASGLLYRGNRYYFVVALHYDIAVTIWSVVAMYIWKELFHEWVYTDDSKLARAIFRHVSPVLECHLALRVGILLKNYLVLLVATSYLWRPYLQRVQSSILAQYILLLLTDYCTKGYCNPGDQNFAIEMGRQGIKEGKNISLYAVSKAMGFIPRNKLGHTFFRQLA-------GSNTLDSSKDATTLGNFLFDQLIVRSRPPPARPXXXXXXXXXXXXXXXXXXXXGSIRMSRGSHGGAGTYRALPRFGGQMQKRNSEPMAVPASPRTPVASPKRFSMFEAGRPQVEGMPLANGGGGGAAAGKVPVAKAVSSKASGTLSKLVPVAGRAALVSSGRGGAGGGTESSDVRGDDAGTTSSANTSPG--RSRVRRRKAGTCVDGVDLSIEVASGGETEATGDSGTEWESPEQP----------------VPSMSPSGRDTRIDRARILAQNRSVFTSCSSVASSSNYGDKECGDGVDGEGRQKDQEDRVKGQAARSGETKAEKKEKPKQEEVLVRKTFCPALEPELLDVAFKIFDLDNSGTVTKEEMVLGVVGTFKDHRSLAHTLQDSEHIAQKLGLIIMCVILFILFFVWLSIWGADVVSLSVTFASFLIAFSFMIGTAASNLMSAVLFIFVSRLYDVGDRVHIYSGSNTQGEEPTNVTVVKVDLMTTVFKRWDEQVFYMPNHLLATKTIVNIQRTAHQWHEFMIQVAATTTPEKLTALQTSLQEFSKSKDKPEGLYTRMGFSLVRIEDSTKLTIRITFRQRGNWQNMEKKWACQSMCTWAIKSACDSLNISYFLPEVPVQMKK 3141
            P+  F ARLFA++  T  S +L+R N  YFVVALH ++   +       ++  L  EW       +    +  V   + C LA  V + L+N+ +LL+A+++LW PYL+R QSSI AQY+LL+LT+YC+ G  N   +  A ++  +  +  K +SLYAV K++ F+  +K+G + F++L        G +T+D+S+ A  LG +L+ +L   +R    +P    XXXXXXXX         S+      HG  G+          +Q                                    PL                +  +S+AS  LS                                         SPG  RSR   +  G C+    L  +        +   +  + ++P  P                VP+  P+             Q  +  TS                                           AE  E P  E  L +  FCPA+   L   AF +FD +  G VTK++++ GVV  +KDH++LA TL DS+ +  KLG II  ++ F++ F+WLSIWG   +S+S+TF+SFL++F+FM G+ A+ L+ +++F+FV+  YDVGDR+ I     T         VVK++L+TTVF+R D Q   MPN LL+   ++N+QR+ + W EF++ V+ATT   KL +L+  +Q+F+       G+Y ++GF+   I+DS KL +RI  + R NW +++++W   +    AI   C  L I+ F P V   M K
Sbjct:  254 PLFNFLARLFAYLSITSTSRILFRFNALYFVVALHQELTNIL-------VFGLLLAEWEQWIQPLVIDKAYSAVHNTILCLLAAAVAVFLRNFTLLLIASTHLWLPYLERTQSSIFAQYVLLILTEYCSTGIINAEFEALASQIAAKTARWSK-LSLYAVYKSVSFVQHHKVGGSVFKELGLNAEGDGGEDTIDNSRQARALGEYLYKRLGDAARAGTWQPPYKPXXXXXXXXPRRAYLPIQSLL-----HGHLGSKSTTACHHTSLQXXXXXXXXXXXXXXXXXXXXXXXXXXXX---XXXXXPL----------------RRTASEASLALS-----------------------------------------SPGSPRSRAGTKDDGPCLSPPPLLAQPLPLPLXPSVTAAPLQPQAPPPPSPILHGLLASLRRNSNVPAPQPAXXXXXXXXXXXXKQEGTPPTSPXXXXXXXXXXXXXXXXXX-------------------XXXMAAENAELPNIE--LAK--FCPAMSTTLRPHAFALFDKEQHGVVTKDDLLQGVVSIYKDHKNLARTLADSQQMVNKLGQIIGALMSFVMIFIWLSIWGYQFLSISITFSSFLLSFTFMFGSTATALVESIIFVFVTSAYDVGDRLMIPDIMQTSRYH--GCKVVKINLLTTVFERGDGQSLIMPNKLLSQMALINLQRSGNMWFEFVVGVSATTPLHKLHSLKGRMQKFANEDS--SGIYHKIGFTFTEIQDSAKLVVRINVQGRFNWGDVKRRWEVHNATCQAIAHHCAELGIT-FTPPVQHHMVK 968          

BLAST of mRNA_Ecto-sp9_F_contig1068.606.1 vs.
Match: Mechanosensitive (ion channel-domain-containing protein n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835YNU8_9STRA)

HSP 1 Score: 203 bits (517), Expect = 4.510e-49
Identity = 255/925 (27.57%), Postives = 396/925 (42.81%), Query Frame = 1

Query:  520 GSLVNREVPQEDEPKEPIEPELMI----GEIQSIRLHEEIALVRRAIVLIMLSLVAAVLVRAFAGELDKDVNIREVVFFV-----SLLLLSFPVIRFTARLFAFVITTRASGLLYRGNRYYFVVALHYDIAVTIWSVVAMYIWKELFHEWVYTDDSKLARAIFRHVSPVLECHLALRVGILLKNYLVLLVATSYLWRPYLQRVQSSILAQYILLLLTDYCTKGYCNPGDQNFAIEMGRQGIKEGKNISLYAVSKAMGFIPRNKLGHTFFRQLAGSNTLDSSKDATTLGNFLFDQLI-----VRSRPPPARPXXXXXXXXXXXXXXXXXXXXGSIRMSRGSHGGAGTYRALP----RFGGQMQKRNSEPMAVPASPRTPVASPKRFSMFEAGRPQVEGMPLANGGGGGAAAGKVPVAKAVSSKAS------------------------GTLSKLVPVAGRAALVSSGRGGAGGGTESSDVRGDDAGTTSSANTSPGRSRVRRRKAGTCVDGVDLSIEVASGGETEATGDSGTEWESPEQPVPSMSPSGRDT--RIDRARILAQNRSVFTSCSSVASSSNYGDKECGDGVDGEGRQKDQEDRVKGQAARSGETKAEKKEKPKQEEVLVRKTFCPALEPELLDVAFKIFDLDNS----GTVTKEEMVLGVVGTFKDHRSLAHTLQDSEHIAQKLGLIIMCVILFILFFVWLSIWGADVVSLSVTFASFLIAFSFMIGTAASNLMSAVLFIFVSRLYDVGDRVHIYSGSNTQGEEPTNVTVVKVDLMTTVFKRWDEQVFYMPNHLLATKTIVNIQRTAHQWHEFMIQVAATTTPEKLTALQTSLQEFSKSKDKPEGLYT----RMGFSLVRIEDSTKLTIRITFRQRGNWQNMEKKWACQSMCTWAIKSACDSLNISYFLPEVPVQMK 3138
            G  +  E   EDEP++  + E  +    GE+  + L +E     R +V  +L  +A     A   ++   + +R    FV      + + S+P   FTA     ++    +      N  YF V LHY  A  +W+V     WK    E+       +  +  R V  VL C++  R+  LL    +L +A  Y+WRPYL+RVQ++I AQY++L+LTDY      N  DQ  A++         K +S+YA+S+AMGFIP+NKL H  F   AGS  +DS + A      LFD+L+     +     PA P  XXXXXXXXXX        GS   +R +    G  R  P    R     Q R    + V A+     A+P   S+ EA R +                G    A A  + +S                                   + +L +S    A G T S D RG  A T        G   VR   A    +   L+ E           D G E    +        S RDT  R++ A +  Q R          +     D +  + V           R    A      +++ ++ P   + L      P +  +L      + D D +      +T+ +    +V  ++  RSLA TL+D E I  KLG +I  V  FI  FV+L I+  +++ L VT+ S ++AFSF+ G++A+N   + +FIFV+R YDVGDRV+I    N +     N  V +++++TTVF+RWD+    +PN  LA + I N++R+        + V+  T   KL AL+  +QE++++   P  LY+     + +   R+   T + +   + Q+G+WQ+   K     + +  +   C  L + Y LP   V +K
Sbjct:  274 GEAIGLEPDAEDEPEDDEDSENAVFEHLGELPMMTLRKE----ARFLVKYLLIALAIFAPGAIIEKVHGCILVRGQCLFVWASLAGMAVASYP---FTAMAIKTLVMVLCNSRFIHSNHVYFFVRLHYASAQLMWAVFIYLFWKYGLAEY---KSPNMQESTDRAVGRVLACYMVSRIVYLLNKMSLLELANRYMWRPYLERVQANIFAQYVILILTDYSLGYVRNDQDQYIALQFSENQGNWDK-MSMYAISRAMGFIPKNKLRHPVFAGSAGSVAMDSKRSARMFAGLLFDRLVNGPLDLARNDGPA-PATXXXXXXXXXXPKSPPAELGSPPRARDADTTGGVRRRSPSPARRVSSPPQSRGVVEVGVDAA--VLRAAPSLHSVQEAPRQR----------------GNSTTATAAVNASSHXXXXXXXXLXXXXXXXVDRXXXXXXXXXXXXXXXKESLSNSDAKYARGST-SGDRRGSKASTQ-------GTGGVRHWNALKPPERKSLAAEPFQ--------DRGAEIR--DAFTDDAEESHRDTLKRLEAAHLREQMRKRALGLRLETAIEENEDDQAEEAV-----------RPYSVAELKSPDESDDEDDPSPPKTLTLWMLRPVMIKQLAVKTMALLDGDTAVACDAEITRHKFQNAMVTIYQQRRSLALTLKDYESILIKLGYVISLVWSFIALFVFLWIFDQNILGLGVTWVSLVVAFSFLFGSSAANFFESCVFIFVTRAYDVGDRVYIKDELNDEA----NYVVQRINMLTTVFRRWDDHYTILPNQQLAKREIRNMRRSGDGVMLMTLLVSIATPRAKLEALKERMQEYARAVTGP--LYSCDPNSVDYCCRRVISCTNMEVFFVYSQKGSWQDNYLKVKNFHLMSLRLAEVCHELGLEYALPTQGVTLK 1133          

BLAST of mRNA_Ecto-sp9_F_contig1068.606.1 vs.
Match: EF-hand (domain-containing protein n=1 Tax=Fonticula alba TaxID=691883 RepID=A0A058ZGC3_FONAL)

HSP 1 Score: 149 bits (377), Expect = 2.770e-32
Identity = 105/360 (29.17%), Postives = 182/360 (50.56%), Query Frame = 1

Query: 2263 LVRKTFCPALEPEL-LDVAFKIFDLDNSGTVTKEEMVLGVVGTFKDHRSLAHTLQDSEHIAQKLGLIIMCVILFILFFVWLSIWGADVVSLSVTFASFLIAFSFMIGTAASNLMSAVLFIFVSRLYDVGDRVHIYSGSNTQGEEPTNVTVVKVDLMTTVFKRWDEQVFYMPNHLLATKTIVNIQRTAHQWHEFMIQVAATTTPEKLTALQTSLQEFSKSKDKPEGLYTRMGFSLVRIEDSTKLTIRITFRQRGNWQNMEKKWACQSMCTWAIKSACDSLNISYFLPEVPVQMKKKSA*FHLFAIWCCRVLAPVMTHSPVCVSMLLGMSALPYGGTSFTCCIGERRSKSLPGLFLDKSRWL 3339
            L R+ F   LEP +  D AF +FD+ +SG + + +M   +   +++ ++L+++L+DS  +   L   +  ++L  L F +L I+G ++ S  V  AS +++ SF+ G +A  +   V+++FV   YDVGDRV I         +  N  V ++++  TVF+RWDEQ F++PNH+L  K ++NI+R+ +Q H F + + A TT E+L  L+  +Q + +S+  PE     + F L  ++   ++ + IT  Q  NWQ+   +W  ++        +C  L I        V +    A   +          P   H P  +S L  M+   Y        +GE  S +LPG+ +  SR +
Sbjct: 1996 LTREDFSLFLEPSVNTDEAFALFDIHSSGQIRRRDMKEALQNIYRERKNLSNSLRDSGTMVNTLDRFVSLMLLVCLLFAFLLIFGTNIESTVVPVASLIVSMSFIFGHSAQRMFDNVIYLFVEHPYDVGDRVFI---------DDHNFIVARMNITATVFRRWDEQYFWIPNHILTDKVVINIRRSGNQRHTFYMFLDADTTSEQLGTLRGMMQGWVESQ--PERFARDVDFILFDVDTHGRMQMLITIGQLSNWQDGAVRWKNRTDSWLFFLQSCRELGIKIIPHGQRVDLTGSVAGLGM---------GPSGGH-PSEMSPLNSMTGSTYASPQ-VADMGEVFSSTLPGVSVFGSRQI 2333          

BLAST of mRNA_Ecto-sp9_F_contig1068.606.1 vs.
Match: EF-hand (domain-containing protein n=2 Tax=Spizellomyces TaxID=4815 RepID=A0A0L0H4I0_SPIPD)

HSP 1 Score: 144 bits (362), Expect = 6.820e-31
Identity = 91/279 (32.62%), Postives = 147/279 (52.69%), Query Frame = 1

Query: 2281 CPALEPELLDVAFKIFDLDNSGTVTKEEMVLGVVGTFKDHRSLAHTLQDSEHIAQKLGLIIMCVILFILFFVWLSIWGADVVSLSVTFASFLIAFSFMIGTAASNLMSAVLFIFVSRLYDVGDRVHIYSGSNTQGEEPTNVTVVKVDLMTTVFKRWDEQVFYMPNHLLATKTIVNIQRTAHQWHEFMIQVAATTTPEKLTALQTSLQEFSKSKDKPEGLYTRMGFSLVRIEDSTKLTIRITFRQRGNWQNMEKKWACQSMCTWAIKSACDSLNISYFLP 3117
            C    P     AF   D D +G++T++E+   V+G +++ R L   ++D      KL      V  F+ F + L I+G  + ++ + F SF++A SF+ GTAA     ++LF+F++  YD GDRV I         +  N+ V +V ++TTVFKR D Q+ Y PN L+A+K I N++R+  Q     +Q+   T  EKL AL+  + +F KS+  P          +  IE+  KL + +  + +GNWQ+  K+W  ++M  +A+K     L I Y  P
Sbjct:  635 CTFSSPTEAADAFAALDQDGNGSITRKEVKEVVIGMYRERRRLFRAMRDLSQALGKLNTFFYFVTSFVTFCIALPIFGISLTAM-LPFTSFILALSFVFGTAARTAFESLLFLFLTHPYDAGDRVVI---------DNQNLLVEEVGVLTTVFKRLDGQLIYAPNTLVASKLIHNLRRSGDQSESIELQLNFDTPEEKLRALKERMIDFVKSE--PREFQGVCDIHISEIENLNKLKVNVILKHKGNWQDGSKRWNRRTMFMFALKKNLLELGIRYATP 901          

BLAST of mRNA_Ecto-sp9_F_contig1068.606.1 vs.
Match: EF-hand (domain-containing protein n=1 Tax=Powellomyces hirtus TaxID=109895 RepID=A0A507E7B5_9FUNG)

HSP 1 Score: 143 bits (361), Expect = 1.170e-30
Identity = 94/276 (34.06%), Postives = 145/276 (52.54%), Query Frame = 1

Query: 2314 AFKIFDLDNSGTVTKEEMVLGVVGTFKDHRSLAHTLQDSEHIAQKLGLIIMCVILFILFFVWL----SIWGADVVSLSVT----FASFLIAFSFMIGTAASNLMSAVLFIFVSRLYDVGDRVHIYSGSNTQGEEPTNVTVVKVDLMTTVFKRWDEQVFYMPNHLLATKTIVNIQRTAHQWHEFMIQVAATTTPEKLTALQTSLQEFSKSKDKPEGLYTRMGFSLVRIEDSTKLTIRITFRQRGNWQNMEKKWACQSMCTWAIKSACDSLNISYFLP 3117
            AF + D D +G++T+ E+   V+G +++ R L   ++D      KL      V  F+ F + L    +I    +  +S+T    F SF++A SF+ GTAA     ++LF+FV+  YD GDRV I S          N+ V +V ++TTVFKR D Q+ Y PN L+ATK I N++R+  Q     IQ+  TT  EKL  L+  L ++ KS+  P          +  IE+  KL + +  + +GNWQ+  K+W  ++M  +A+K     L I Y  P
Sbjct:  766 AFSVLDQDGNGSITRREVKECVIGMYRERRGLFRAMRDLSQALGKLNTFFFFVDGFVTFCIALPAHHAIISIKIFGISLTAMLPFTSFILALSFVFGTAARTAFESLLFLFVTHPYDAGDRVVIDS---------QNLLVEEVGVLTTVFKRMDGQLIYAPNTLVATKLIHNLRRSGDQSECIEIQLDFTTPEEKLRQLRERLVDYVKSE--PREFVGACDVHIADIENVNKLKLNVILKHKGNWQDGSKRWGRRTMFMYALKHHLTELEIRYLTP 1030