mRNA_Ecto-sp9_F_contig10770.674.1 (mRNA) Ectocarpus species9 EcSCA_722f female

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_Ecto-sp9_F_contig10770.674.1
Unique NamemRNA_Ecto-sp9_F_contig10770.674.1
TypemRNA
OrganismEctocarpus species9 EcSCA_722f female (Ectocarpus species9 EcSCA_722f female)
Homology
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BLAST of mRNA_Ecto-sp9_F_contig10770.674.1 vs.
Match: Alpha-(1,6)-fucosyltransferase, (family GT23 n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7G435_ECTSI)

HSP 1 Score: 548 bits (1411), Expect = 1.740e-193
Identity = 287/312 (91.99%), Postives = 291/312 (93.27%), Query Frame = 1 
Query:    1 MLATMADSSHRNEKSRTVRRGGRPRRGGGTPRAALRASVVLAVLGRCSSYVRGAVAEAEVAEAEDGSKTPALSMELDVGDESRVTTADGSEGGDAAAAATAAA-PAVLFSSEWAAEYARGEEGCDLARGSFMWRGHGLGSNVNNMLNAWVYALSVEKWSDMTVVVDENQMKRVDCGEESGGEAQTGWDCLFKPMPHLCTFPSAESWKEYMVSKDLPEADLVEVARLDQDAVRFHPEEIVASLEGSGVDHIAALAAMAKYLWSNVTPWLQADVDFVTRASPSDVFQESPFLGLHIRRGDKVTQGE-ASMYLCK 930
            MLATMADSSHRNEKSRTVRRGGRPRRGG TPRAAL+ASVVLAVLGRCSSYVR   AEAEVAEAEDGSKTPALSMELDVGDESRVT ADGSEGGDA         PAVLFSSEWA EYARGEEGCDLARGSFMWRG GLGSN+NNMLNAWVYALSVEKWSDMTVVV ENQMK VDCGEESGGEAQTGWDC+FKPMPHLCTFPSAESWKEYMVSKDLPEA+LVEVARLDQDAVRFHPEEIVASLEGSGVDHIAALAAMAKYLWSNVTPWLQADVDFVTRASPSDVFQESPFLGLHIRRGDKVT G  ASMYLCK
Sbjct:    1 MLATMADSSHRNEKSRTVRRGGRPRRGGCTPRAALQASVVLAVLGRCSSYVR---AEAEVAEAEDGSKTPALSMELDVGDESRVTAADGSEGGDAXXXXXXXXXPAVLFSSEWAVEYARGEEGCDLARGSFMWRGQGLGSNINNMLNAWVYALSVEKWSDMTVVVGENQMKWVDCGEESGGEAQTGWDCIFKPMPHLCTFPSAESWKEYMVSKDLPEAELVEVARLDQDAVRFHPEEIVASLEGSGVDHIAALAAMAKYLWSNVTPWLQADVDFVTRASPSDVFQESPFLGLHIRRGDKVTHGRRASMYLCK 309
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BLAST of mRNA_Ecto-sp9_F_contig10770.674.1 vs.
Match: GT23 (protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JTL0_9PHAE)

HSP 1 Score: 528 bits (1360), Expect = 7.500e-186
Identity = 277/310 (89.35%), Postives = 288/310 (92.90%), Query Frame = 1 
Query:    1 MLATMADSSHRNEKSRTVRRGGRPRRGGGTPRAALRASVVLAVLGRCSSYVRGAVAEAEVAEAEDGSKTPALSMELDVGDESRVTTADGSEGGDAAAAATAAAPAVLFSSEWAAEYARGEEGCDLARGSFMWRGHGLGSNVNNMLNAWVYALSVEKWSDMTVVVDENQMKRVDCGEESGGEAQTGWDCLFKPMPHLCTFPSAESWKEYMVSKDLPEADLVEVARLDQDAVRFHPEEIVASLEGSGVDHIAALAAMAKYLWSNVTPWLQADVDFVTRASPSDVFQESPFLGLHIRRGDKVTQGEASMYLCK 930
            MLA MADSS RNEK+RTVRRGGRPRR GGTP AALRASVVL +LGRCSSYVRGAVAEAE     DG +TPALS+ELDVGDESRV TADGSEGG++AAAA A APAVLFSSEWAAEYARGE GCDLARGSFMWRGHGLGSNVNNMLNAWVYALSVEKWSDMTVVVDENQMK+VDC EESGGEAQ GWDCLFKPMPHLCTFPSAE   E+MVSK LP+ADLVE ARLDQDAVRFHPEEIVASLEGSGVDHIAALAAMAKYLWSNVTPWLQADVDFVTRASPSDVFQESPFLGLHIRRGDKV+QGEASMYLC+
Sbjct:    1 MLAMMADSSDRNEKARTVRRGGRPRRVGGTPAAALRASVVLVMLGRCSSYVRGAVAEAE-----DGLETPALSLELDVGDESRVNTADGSEGGESAAAA-ATAPAVLFSSEWAAEYARGEAGCDLARGSFMWRGHGLGSNVNNMLNAWVYALSVEKWSDMTVVVDENQMKQVDCEEESGGEAQAGWDCLFKPMPHLCTFPSAE---EHMVSKGLPKADLVEAARLDQDAVRFHPEEIVASLEGSGVDHIAALAAMAKYLWSNVTPWLQADVDFVTRASPSDVFQESPFLGLHIRRGDKVSQGEASMYLCE 301
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BLAST of mRNA_Ecto-sp9_F_contig10770.674.1 vs.
Match: GT23 (protein n=2 Tax=Ectocarpus TaxID=2879 RepID=A0A6H5LR29_9PHAE)

HSP 1 Score: 66.2 bits (160), Expect = 8.530e-9
Identity = 29/50 (58.00%), Postives = 35/50 (70.00%), Query Frame = 1 
Query:  763 MAKYLWSNVTPWLQADVDFVTRASPSDVFQESPFLGLHIRRGDKVTQGEA 912
            MA+ LW N+TPWLQ DV         DVF+  PF+GLH+RRGDKV +GEA
Sbjct:  105 MARLLWKNLTPWLQEDVRKALERPDLDVFRRKPFVGLHVRRGDKVGEGEA 154
The following BLAST results are available for this feature:
BLAST of mRNA_Ecto-sp9_F_contig10770.674.1 vs.
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Ectocarpus species9 EcSCA_722f female vs UniRef90)
Total hits: 3
Match NameE-valueIdentityDescription
Alpha-(1,6)-fucosyltransferase,1.740e-19391.99family GT23 n=1 Tax=Ectocarpus siliculosus TaxID=2... [more]
GT237.500e-18689.35protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=... [more]
GT238.530e-958.00protein n=2 Tax=Ectocarpus TaxID=2879 RepID=A0A6H5... [more]
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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
Ecto-sp9_F_contig10770contigEcto-sp9_F_contig10770:988..4283 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Ectocarpus species9 EcSCA_722f female vs UniRef902022-09-19
OGS1.0 of Ectocarpus species9 EcSCA_722f female2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score550.8
Seed ortholog evalue4e-154
Seed eggNOG ortholog2880.D7G435
KEGG koko:K00717
KEGG ReactionR05988,R09319
KEGG Pathwayko00510,ko00513,ko00533,ko01100,ko05202,map00510,map00513,map00533,map01100,map05202
KEGG ModuleM00075
EggNOG free text desc.glycoprotein 6-alpha-L-fucosyltransferase activity
EggNOG OGsKOG3705@1,KOG3705@2759
EC2.4.1.68
COG Functional cat.M
CAZyGT23
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko00002,ko01000,ko01003
Hectar predicted targeting categoryother localisation
Exons4
Model size930
Cds size930
Stop0
Start1
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_Ecto-sp9_F_contig10770.674.1prot_Ecto-sp9_F_contig10770.674.1Ectocarpus species9 EcSCA_722f femalepolypeptideEcto-sp9_F_contig10770 988..4283 +


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622928296.9951026-CDS-Ecto-sp9_F_contig10770:987..14141622928296.9951026-CDS-Ecto-sp9_F_contig10770:987..1414Ectocarpus species9 EcSCA_722f femaleCDSEcto-sp9_F_contig10770 988..1414 +
1692277825.9506273-CDS-Ecto-sp9_F_contig10770:987..14141692277825.9506273-CDS-Ecto-sp9_F_contig10770:987..1414Ectocarpus species9 EcSCA_722f femaleCDSEcto-sp9_F_contig10770 988..1414 +
1622928297.0458293-CDS-Ecto-sp9_F_contig10770:1796..19781622928297.0458293-CDS-Ecto-sp9_F_contig10770:1796..1978Ectocarpus species9 EcSCA_722f femaleCDSEcto-sp9_F_contig10770 1797..1978 +
1692277825.9673133-CDS-Ecto-sp9_F_contig10770:1796..19781692277825.9673133-CDS-Ecto-sp9_F_contig10770:1796..1978Ectocarpus species9 EcSCA_722f femaleCDSEcto-sp9_F_contig10770 1797..1978 +
1622928297.0599806-CDS-Ecto-sp9_F_contig10770:2995..31451622928297.0599806-CDS-Ecto-sp9_F_contig10770:2995..3145Ectocarpus species9 EcSCA_722f femaleCDSEcto-sp9_F_contig10770 2996..3145 +
1692277825.978845-CDS-Ecto-sp9_F_contig10770:2995..31451692277825.978845-CDS-Ecto-sp9_F_contig10770:2995..3145Ectocarpus species9 EcSCA_722f femaleCDSEcto-sp9_F_contig10770 2996..3145 +
1622928297.0767193-CDS-Ecto-sp9_F_contig10770:4112..42831622928297.0767193-CDS-Ecto-sp9_F_contig10770:4112..4283Ectocarpus species9 EcSCA_722f femaleCDSEcto-sp9_F_contig10770 4113..4283 +
1692277825.9884999-CDS-Ecto-sp9_F_contig10770:4112..42831692277825.9884999-CDS-Ecto-sp9_F_contig10770:4112..4283Ectocarpus species9 EcSCA_722f femaleCDSEcto-sp9_F_contig10770 4113..4283 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_Ecto-sp9_F_contig10770.674.1

>prot_Ecto-sp9_F_contig10770.674.1 ID=prot_Ecto-sp9_F_contig10770.674.1|Name=mRNA_Ecto-sp9_F_contig10770.674.1|organism=Ectocarpus species9 EcSCA_722f female|type=polypeptide|length=310bp
MLATMADSSHRNEKSRTVRRGGRPRRGGGTPRAALRASVVLAVLGRCSSY
VRGAVAEAEVAEAEDGSKTPALSMELDVGDESRVTTADGSEGGDAAAAAT
AAAPAVLFSSEWAAEYARGEEGCDLARGSFMWRGHGLGSNVNNMLNAWVY
ALSVEKWSDMTVVVDENQMKRVDCGEESGGEAQTGWDCLFKPMPHLCTFP
SAESWKEYMVSKDLPEADLVEVARLDQDAVRFHPEEIVASLEGSGVDHIA
ALAAMAKYLWSNVTPWLQADVDFVTRASPSDVFQESPFLGLHIRRGDKVT
QGEASMYLCK
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mRNA from alignment at Ecto-sp9_F_contig10770:988..4283+

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_Ecto-sp9_F_contig10770.674.1 ID=mRNA_Ecto-sp9_F_contig10770.674.1|Name=mRNA_Ecto-sp9_F_contig10770.674.1|organism=Ectocarpus species9 EcSCA_722f female|type=mRNA|length=3296bp|location=Sequence derived from alignment at Ecto-sp9_F_contig10770:988..4283+ (Ectocarpus species9 EcSCA_722f female)
ATGCTGGCGACGATGGCGGATTCCTCCCACAGGAACGAAAAAAGCAGAAC
CGTCCGCCGCGGGGGGCGGCCCCGCCGTGGCGGCGGCACACCAAGGGCGG
CGCTGCGGGCTTCGGTGGTGCTGGCCGTGCTCGGGAGATGTAGTAGCTAC
GTCCGTGGCGCGGTCGCAGAGGCGGAGGTCGCAGAGGCGGAGGATGGGTC
GAAGACGCCCGCGCTCTCTATGGAGCTGGACGTTGGTGACGAATCGCGGG
TGACTACCGCTGACGGATCGGAAGGCGGGGATGCAGCAGCGGCGGCGACG
GCGGCCGCGCCGGCAGTGCTGTTTTCCTCGGAGTGGGCGGCGGAGTACGC
GCGGGGGGAGGAAGGTTGCGACCTGGCCAGGGGGAGCTTCATGTGGAGGG
GGCATGGCTTGGGCAGCAACGTGAACAGTGAGTGGGAGAGAGAGCGGGAC
GGACTTGCCCCGGGTTTGAACGCTTGATCGCTCTCTGACAACATCATCTC
GGTCGATTTTGGACACCGCAAAATGGAATGCCATTGCCTGTTTTCATGTC
GTCGGGACGCATCATGCTGTCGGAATACAGAACACACACCGAACATGATC
GGAGGCAAAAACTCGCCACACCTTATTTTGCTGGGTCGGATATTTTCCTC
TCGTATAGCTGTTTCCGTTGCTGAGTCATTATGTCATCCTAGCCTGCTTG
TACAGTTGCCGGCCACTCTTCTCTCTGCCGCCGCCGGCCCTCCATCGCAT
CCACACCCCACGTGTTACGCCGCGTCTGCCTTCTGTGTCTGGGGTCGACA
AACTCTCAGACATGCTGAACGCGTGGGTGTACGCCCTCTCAGTGGAGAAG
TGGAGCGACATGACCGTGGTCGTGGACGAAAACCAGATGAAACGGGTGGA
CTGCGGGGAGGAGAGCGGTGGAGAGGCTCAGACGGGCTGGGACTGCCTTT
TCAAGCCTATGCCGCATCTTTGCACCTTCCCCTCGGCGGAGGTGAGTGGG
GGCGACCAGGGGCACCGGGGCCCGGGGACAAGAAACGACGAAATCCGGCC
GAGGCTCAACTTCTTATTATTTCATCGCCATAGTATGGTGGAGGATCCAC
CGCTGAGGTGTGATTCTTCGACCTATGTTCTTCCCTGTCGTGAAACATTT
CGAGCCTCTGCTGTTTCTTGAACGAAGCGTTTCTTAAACTGCGGGCCCGG
CTTGGAGAGATGGAGATTCGGTTGAGCGAAAGCCGTTCAGCGGCCGAGCA
ATCTCCTCGTGTGGGACCGAGCCCCGGATCGTACTACAGTAGTGGGCCTT
TAACAACAACGCCGGTTGAATAGCGCCGGTGGCCCTGTGTAGAGCCGGTG
AAGTTGCTGGGCATAGACTGAATCACCAAGTCATGCTGTACTCTCAATAG
CGCCGGTCGATTCTCCGGGAACATGCCGGCGTTTTTGAGGGCCCGCTGGT
GTGGCAAGACTGTAGACATGTCCTCCCGCAGAGAAGGCAAGGGCGGCGAA
ATGATGTGGGTGGATGCTGCGAGCAATGATAGTATTGGTGGCCTGGACGG
TCACATTAATAGAGATCTCAGTCGTTCTTTGGCTTCGCCACCGCTACGTT
TGTACATGGCACCATGACGCACCCCGGAAGTGGCTGATTTGGTTACCCGC
AAGCAGGAAAAATGTGCTCCAACAGGCTGTGTTAGCGGTTTGTCCACCCC
GCGAGTGATATCCTGTCCTCTTTCGTTTCCGCGTTTATGGAGGAACACCA
TTGCCACGCTTTGCCGTGCTGTACACTTGCGGGTGCACTTTTTTTCCTGT
GAGCAGTTATGTCGAGGAATCAAACCTGGCGGACTCGTCCAATCACGTTA
GCAGGAGACGCACCTTATTGCAGGGCCTCGCTAACCCTGATCCAAGAACT
ACCAGGTCCCAATTGGGAGGAACCAACCCCCTTGCATTCGAACGTTTCGG
AGGTATTCATGAATCTGCCAACCATCGCCCAACGACTCCTTTTTTGCTAC
TTTTCAAGTCCTGGAAGGAGTACATGGTGTCGAAAGACCTACCCGAAGCG
GACCTGGTTGAGGTGGCGAGGCTGGACCAGGACGCCGTACGGTTCCACCC
GGAAGAGATAGTGGCGTCGTTGGAAGGGTCGGGGGTCGACCACATCGCTG
CCTTGGCGGTGCGTGTTTGGACGTTGTTGTTGTTTGTTGCATTGTTATTT
CGCGAATAACTCATAACCAGCAATGTACCCAGCAACGCCTGTCTCGGCCC
CACTGGCAAGATGGGAGAAATCGTTTCAGGGGGCGCGATAGGTTTGAGAT
TTTGTTGCTGACTGGACGCGGGCTTTGGGCGAGCAGCAGCAGTGCCACCG
TGGGCGAGATTGGTTTTGAGTCCAAGCACGTATAGTATGTAGTACAGTTT
CAGTGTGGCCTTGTTCGATCGAGGTACATCAAGTGCATTTGACAGCGGCC
GGCGTCACCAAGTCACAGCAGGACCAGGACAACGCGTGTTGTTGTTGGTG
GCCTGCCTGTTCTTGCTGTCAGTCGAAGAATGGGTTCGCTCGGGGTGTTC
CCCCCCCCCCCCCCCCCCCCCCACAGCGGCGCCGGGGGGGAAAGGGGCTG
CCGCGCCCCAAAAGACACACCCCACCCCCCCCCAGGGCGCNNNNNNNNNN
CCAGGACCACGTGGGTGGTTGGTGGGGGCCCGCCTTTTCCTCCGGGCCAG
CCGAGAAAGGGTTCGCGCGGGGGGTTTCCCCCCCCCCCCCCCCCCGCCCC
GGAAAAGGGGAACCAGCGGGTATAGATCTCGCTACCATAGAACAAAGCCA
CGCACACCCCAGAGGGACGAGTCTTTGTGATCCCGGGCTAGAGGTCTCCA
GCCGAGCGAACATTTCAAAGGAAGATGCAGATAATATTGTAGCCTGCGAG
CGCGAAATTACCTAGCGACTACCATTTTTTATCATATTCGGATGGACTTT
GAGATACTCATATGACACGAACAAGAAAGCACGCACCCGATACCTCTCGG
TTTTGTGTTGAACAACAGTTGTAGACGCGCCTTAGATCCCGCCCACTCCC
CACTTGCACGGCATCCCGCCCCCCCACCGAAAGCTGACACTCGCTCGCGA
AAAAAACACACGATGACGGACGCAGGCCATGGCCAAGTACTTGTGGAGCA
ACGTCACCCCGTGGCTCCAGGCCGACGTCGACTTCGTCACGCGCGCCTCG
CCCTCGGACGTGTTCCAGGAATCCCCGTTCCTGGGCCTGCACATACGGCG
GGGTGACAAGGTCACGCAAGGAGAGGCGTCCATGTACCTGTGTAAG
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Coding sequence (CDS) from alignment at Ecto-sp9_F_contig10770:988..4283+

>mRNA_Ecto-sp9_F_contig10770.674.1 ID=mRNA_Ecto-sp9_F_contig10770.674.1|Name=mRNA_Ecto-sp9_F_contig10770.674.1|organism=Ectocarpus species9 EcSCA_722f female|type=CDS|length=1860bp|location=Sequence derived from alignment at Ecto-sp9_F_contig10770:988..4283+ (Ectocarpus species9 EcSCA_722f female)
ATGCTGGCGACGATGGCGGATTCCTCCCACAGGAACGAAAAAAGCAGAAC
CGTCCGCCGCGGGGGGCGGCCCCGCCGTGGCGGCGGCACACCAAGGGCGG
CGCTGCGGGCTTCGGTGGTGCTGGCCGTGCTCGGGAGATGTAGTAGCTAC
GTCCGTGGCGCGGTCGCAGAGGCGGAGGTCGCAGAGGCGGAGGATGGGTC
GAAGACGCCCGCGCTCTCTATGGAGCTGGACGTTGGTGACGAATCGCGGG
TGACTACCGCTGACGGATCGGAAGGCGGGGATGCAGCAGCGGCGGCGACG
GCGGCCGCGCCGGCAGTGCTGTTTTCCTCGGAGTGGGCGGCGGAGTACGC
GCGGGGGGAGGAAGGTTGCGACCTGGCCAGGGGGAGCTTCATGTGGAGGG
GGCATGGCTTGGGCAGCAACGTGAACAATGCTGGCGACGATGGCGGATTC
CTCCCACAGGAACGAAAAAAGCAGAACCGTCCGCCGCGGGGGGCGGCCCC
GCCGTGGCGGCGGCACACCAAGGGCGGCGCTGCGGGCTTCGGTGGTGCTG
GCCGTGCTCGGGAGATGTAGTAGCTACGTCCGTGGCGCGGTCGCAGAGGC
GGAGGTCGCAGAGGCGGAGGATGGGTCGAAGACGCCCGCGCTCTCTATGG
AGCTGGACGTTGGTGACGAATCGCGGGTGACTACCGCTGACGGATCGGAA
GGCGGGGATGCAGCAGCGGCGGCGACGGCGGCCGCGCCGGCAGTGCTGTT
TTCCTCGGAGTGGGCGGCGGAGTACGCGCGGGGGGAGGAAGGTTGCGACC
TGGCCAGGGGGAGCTTCATGTGGAGGGGGCATGGCTTGGGCAGCAACGTG
AACAACATGCTGAACGCGTGGGTGTACGCCCTCTCAGTGGAGAAGTGGAG
CGACATGACCGTGGTCGTGGACGAAAACCAGATGAAACGGGTGGACTGCG
GGGAGGAGAGCGGTGGAGAGGCTCAGACGGGCTGGGACTGCCTTTTCAAG
CCTATGCCGCATCTTTGCACCTTCCCCTCGGCGGAGACATGCTGAACGCG
TGGGTGTACGCCCTCTCAGTGGAGAAGTGGAGCGACATGACCGTGGTCGT
GGACGAAAACCAGATGAAACGGGTGGACTGCGGGGAGGAGAGCGGTGGAG
AGGCTCAGACGGGCTGGGACTGCCTTTTCAAGCCTATGCCGCATCTTTGC
ACCTTCCCCTCGGCGGAGTCCTGGAAGGAGTACATGGTGTCGAAAGACCT
ACCCGAAGCGGACCTGGTTGAGGTGGCGAGGCTGGACCAGGACGCCGTAC
GGTTCCACCCGGAAGAGATAGTGGCGTCGTTGGAAGGGTCGGGGGTCGAC
CACATCGCTGCCTTGGCGTCCTGGAAGGAGTACATGGTGTCGAAAGACCT
ACCCGAAGCGGACCTGGTTGAGGTGGCGAGGCTGGACCAGGACGCCGTAC
GGTTCCACCCGGAAGAGATAGTGGCGTCGTTGGAAGGGTCGGGGGTCGAC
CACATCGCTGCCTTGGCGGCCATGGCCAAGTACTTGTGGAGCAACGTCAC
CCCGTGGCTCCAGGCCGACGTCGACTTCGTCACGCGCGCCTCGCCCTCGG
ACGTGTTCCAGGAATCCCCGTTCCTGGGCCTGCACATACGGCGGGGTGAC
AAGGTCACGCAAGGAGAGGCGTCCATGTACCTGTGTAAGGCCATGGCCAA
GTACTTGTGGAGCAACGTCACCCCGTGGCTCCAGGCCGACGTCGACTTCG
TCACGCGCGCCTCGCCCTCGGACGTGTTCCAGGAATCCCCGTTCCTGGGC
CTGCACATACGGCGGGGTGACAAGGTCACGCAAGGAGAGGCGTCCATGTA
CCTGTGTAAG
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