mRNA_Ecto-sp9_F_contig10381.376.1 (mRNA) Ectocarpus species9 EcSCA_722f female

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_Ecto-sp9_F_contig10381.376.1
Unique NamemRNA_Ecto-sp9_F_contig10381.376.1
TypemRNA
OrganismEctocarpus species9 EcSCA_722f female (Ectocarpus species9 EcSCA_722f female)
Homology
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BLAST of mRNA_Ecto-sp9_F_contig10381.376.1 vs.
Match: PPR_long (domain-containing protein n=2 Tax=Ectocarpus TaxID=2879 RepID=D7G0M5_ECTSI)

HSP 1 Score: 338 bits (867), Expect = 4.340e-105
Identity = 290/352 (82.39%), Postives = 293/352 (83.24%), Query Frame = 1 
Query:    1 DVAGTTAAVPVKKNRYCVNAALDACGKAGRVQEALGLMRDARASGIELDVVSYNCAIPACVTGGDWELALSMIREMEAEYGIKPNHXXXXXXXXXXXXXXXXXXXXXXXXXMKDGGVPLKTSVYSAVFNAFRRVDHWQAAASTLREIHDWPVKPNVFNYNLAICTCTDCGEWEEALDLLHFMREVGGVEXXXXXXXXXXXXXXXXXXXXXXXXXFREMSEVGIKXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXTEVVDGSGAANGXXXXXXXXPPRDPVEVADACSYNTALSACEVGEQWERALSLLRDMPKRGVEPVVI 1056
            D+    A +    N YCVNAALDACGKAGRVQEALGLMRDARASGIELDVVS                    IREMEAEYGIKPNH                         MKDGGVPLKTSVYSAVFNAFRRVDHWQAAASTLREIHDWPVKPNVFNYNLAICTCTDCGEWEEALDLLHFMREVGGVEXXXXXXXXXXXXXXXXXXXXXXXXXFREMSEVGIKXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXTEVVDGSGAANGXXXXXXXX   DPVEVADACSYNTALSACEVGEQW RALSLLRDMPKRGV+PVVI
Sbjct:  145 DILARMAELGAMPNSYCVNAALDACGKAGRVQEALGLMRDARASGIELDVVSXXXXXXXXXXXXXXXXXXXXIREMEAEYGIKPNHITYQAAIKVCGRCGKADEAASLLIEMKDGGVPLKTSVYSAVFNAFRRVDHWQAAASTLREIHDWPVKPNVFNYNLAICTCTDCGEWEEALDLLHFMREVGGVEXXXXXXXXXXXXXXXXXXXXXXXXXFREMSEVGIKXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXTEVVDGSGAANGXXXXXXXX---DPVEVADACSYNTALSACEVGEQWGRALSLLRDMPKRGVKPVVI 493
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BLAST of mRNA_Ecto-sp9_F_contig10381.376.1 vs.
Match: Hypothetical (protein (Fragment) n=1 Tax=Alexandrium catenella TaxID=2925 RepID=A0A7S1SAR9_ALECA)

HSP 1 Score: 53.1 bits (126), Expect = 4.610e-5
Identity = 25/52 (48.08%), Postives = 34/52 (65.38%), Query Frame = 1 
Query:  943 DACSYNTALSACEVGEQWERALSLLRDMPKRGVEPVVISYNTVISACGKCGE 1098
            D  +Y TA+  C+VGE WERA+ LLR M      P +++Y+  ISAC KCG+
Sbjct:   69 DPGNYVTAIRGCKVGEDWERAVRLLRLMHFCDTIPDLMAYSATISACEKCGQ 120
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BLAST of mRNA_Ecto-sp9_F_contig10381.376.1 vs.
Match: Hypothetical (protein n=1 Tax=Symbiodinium sp. CCMP2592 TaxID=631055 RepID=A0A812IFC2_9DINO)

HSP 1 Score: 54.7 bits (130), Expect = 5.530e-5
Identity = 25/47 (53.19%), Postives = 30/47 (63.83%), Query Frame = 1 
Query:  943 DACSYNTALSACEVGEQWERALSLLRDMPKRGVEPVVISYNTVISAC 1083
            D   YN+ L+ACE G QWE  L LL  MP   +EP V+SY+  ISAC
Sbjct:    9 DETGYNSILTACEKGRQWEMVLWLLATMPSLRLEPDVVSYSAAISAC 55
The following BLAST results are available for this feature:
BLAST of mRNA_Ecto-sp9_F_contig10381.376.1 vs.
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Ectocarpus species9 EcSCA_722f female vs UniRef90)
Total hits: 3
Match NameE-valueIdentityDescription
PPR_long4.340e-10582.39domain-containing protein n=2 Tax=Ectocarpus TaxID... [more]
Hypothetical4.610e-548.08protein (Fragment) n=1 Tax=Alexandrium catenella T... [more]
Hypothetical5.530e-553.19protein n=1 Tax=Symbiodinium sp. CCMP2592 TaxID=63... [more]
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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
Ecto-sp9_F_contig10381contigEcto-sp9_F_contig10381:1136..4570 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Ectocarpus species9 EcSCA_722f female vs UniRef902022-09-19
OGS1.0 of Ectocarpus species9 EcSCA_722f female2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score341.3
Seed ortholog evalue5.7e-91
Seed eggNOG ortholog2880.D7G0M5
KEGG koko:K17710
EggNOG free text desc.endonuclease activity
EggNOG OGsKOG4197@1,KOG4197@2759
COG Functional cat.S
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko03016,ko03029
Hectar predicted targeting categoryother localisation
Ec32 ortholog descriptionPentatricopeptide repeat
Ec32 orthologEc-07_001540.1
Exons5
Model size1107
Cds size1107
Stop0
Start0
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_Ecto-sp9_F_contig10381.376.1prot_Ecto-sp9_F_contig10381.376.1Ectocarpus species9 EcSCA_722f femalepolypeptideEcto-sp9_F_contig10381 1136..4570 -


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622928246.3279836-CDS-Ecto-sp9_F_contig10381:1135..12611622928246.3279836-CDS-Ecto-sp9_F_contig10381:1135..1261Ectocarpus species9 EcSCA_722f femaleCDSEcto-sp9_F_contig10381 1136..1261 -
1692277807.5603461-CDS-Ecto-sp9_F_contig10381:1135..12611692277807.5603461-CDS-Ecto-sp9_F_contig10381:1135..1261Ectocarpus species9 EcSCA_722f femaleCDSEcto-sp9_F_contig10381 1136..1261 -
1622928246.3402693-CDS-Ecto-sp9_F_contig10381:1817..21381622928246.3402693-CDS-Ecto-sp9_F_contig10381:1817..2138Ectocarpus species9 EcSCA_722f femaleCDSEcto-sp9_F_contig10381 1818..2138 -
1692277807.5712898-CDS-Ecto-sp9_F_contig10381:1817..21381692277807.5712898-CDS-Ecto-sp9_F_contig10381:1817..2138Ectocarpus species9 EcSCA_722f femaleCDSEcto-sp9_F_contig10381 1818..2138 -
1622928246.354096-CDS-Ecto-sp9_F_contig10381:2591..28371622928246.354096-CDS-Ecto-sp9_F_contig10381:2591..2837Ectocarpus species9 EcSCA_722f femaleCDSEcto-sp9_F_contig10381 2592..2837 -
1692277807.5794525-CDS-Ecto-sp9_F_contig10381:2591..28371692277807.5794525-CDS-Ecto-sp9_F_contig10381:2591..2837Ectocarpus species9 EcSCA_722f femaleCDSEcto-sp9_F_contig10381 2592..2837 -
1622928246.3639376-CDS-Ecto-sp9_F_contig10381:3457..35891622928246.3639376-CDS-Ecto-sp9_F_contig10381:3457..3589Ectocarpus species9 EcSCA_722f femaleCDSEcto-sp9_F_contig10381 3458..3589 -
1692277807.5893455-CDS-Ecto-sp9_F_contig10381:3457..35891692277807.5893455-CDS-Ecto-sp9_F_contig10381:3457..3589Ectocarpus species9 EcSCA_722f femaleCDSEcto-sp9_F_contig10381 3458..3589 -
1622928246.3773015-CDS-Ecto-sp9_F_contig10381:4288..45701622928246.3773015-CDS-Ecto-sp9_F_contig10381:4288..4570Ectocarpus species9 EcSCA_722f femaleCDSEcto-sp9_F_contig10381 4289..4570 -
1692277807.5990279-CDS-Ecto-sp9_F_contig10381:4288..45701692277807.5990279-CDS-Ecto-sp9_F_contig10381:4288..4570Ectocarpus species9 EcSCA_722f femaleCDSEcto-sp9_F_contig10381 4289..4570 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_Ecto-sp9_F_contig10381.376.1

>prot_Ecto-sp9_F_contig10381.376.1 ID=prot_Ecto-sp9_F_contig10381.376.1|Name=mRNA_Ecto-sp9_F_contig10381.376.1|organism=Ectocarpus species9 EcSCA_722f female|type=polypeptide|length=369bp
DVAGTTAAVPVKKNRYCVNAALDACGKAGRVQEALGLMRDARASGIELDV
VSYNCAIPACVTGGDWELALSMIREMEAEYGIKPNHITYQAAIKVCGRCG
KADEAASLLREMKDGGVPLKTSVYSAVFNAFRRVDHWQAAASTLREIHDW
PVKPNVFNYNLAICTCTDCGEWEEALDLLHFMREVGGVEPDVVTYNIVVA
ACGKAGQGEKAIEIFREMSEVGIKPDVISFTTAISACGSCGLSEEALSIF
REMERARVRPNIITHNAVMSACIAAGQWEEALDFFTEVVDGSGAANGGGG
GGGGGPPRDPVEVADACSYNTALSACEVGEQWERALSLLRDMPKRGVEPV
VISYNTVISACGKCGEGDQ
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mRNA from alignment at Ecto-sp9_F_contig10381:1136..4570-

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_Ecto-sp9_F_contig10381.376.1 ID=mRNA_Ecto-sp9_F_contig10381.376.1|Name=mRNA_Ecto-sp9_F_contig10381.376.1|organism=Ectocarpus species9 EcSCA_722f female|type=mRNA|length=3435bp|location=Sequence derived from alignment at Ecto-sp9_F_contig10381:1136..4570- (Ectocarpus species9 EcSCA_722f female)
GACGTCGCCGGCACTACCGCCGCCGTTCCCGTTAAAAAAAACAGGTACTG
CGTGAACGCTGCGCTGGACGCGTGCGGCAAGGCCGGGCGGGTCCAGGAAG
CCCTGGGCCTCATGAGGGACGCGCGGGCGTCGGGGATAGAGCTCGACGTT
GTGAGCTACAACTGCGCTATCCCGGCCTGCGTCACGGGGGGGGACTGGGA
GCTGGCCCTGAGCATGATTCGGGAGATGGAAGCCGAGTACGGCATCAAGC
CGAATCACATCACCTACCAGGCGGCGATAAAGGTGTGCGCTCGCACGGCT
GCTGCTGCTGCTATTGCTGCTGCTATTGCTGCTGCTGTTGTTGCTGCTGC
TGCTGCTGCTGCTGCTGCTGCTGCTGCTGCTGCGGCTGCTGCTGCGGCTG
CTGCGGCTGCTGCGGCTGCTGCGGCTGCTGCTGATGTTGTCGTGCTCGAA
AAGTTGTGAAAATGAACAAACGGCTAGGGTAACAGCAGTGTGTGTAATTT
GACTGACGTAACGTTTTATTTTTTCAGTACCCCGAGAGGTGTTGCCGCGG
AACCCCTGCGGTATGGCTTGGCGCCCAGACGCGGCAGGAATGTTCAGTTC
CTGCAACGACGCTGCCCTGCTTTGCACTCTTCGAGGATGTCTTTGTGCTC
TGCTAAGATAATATATTGTTATTTGCGGCTTTTCTTTTGCCCACGAAGAG
CATGTGTGTCGTGCACGAAAGCTGAGAAACGATAGCTTGGATTTGGTACC
GAGTCACATGGCATGCGTACGTGCATACGACGATGAATAAATTAGGGTTC
GGCTTTCCCCAGTGTGACTCGGGAATTAGCCGCTCGTTAGTCCCTTAGCT
TTGCCCGTTGTAACTCGGGAAAGAGCCGTTCGTAGTCCCATGATCCCGCA
CAACAAGGGATGACGTACTATTAGACCGCCTGCAACAGTTGTACTCAACC
GACGGCGCGCGGATTACGTGATGCGGGGTAGGTGTGCGGGAGGTGCGGCA
AGGCTGACGAGGCGGCTAGTCTCCTGAGAGAGATGAAGGACGGCGGCGTG
CCGCTCAAGACGTCGGTGTACTCGGCGGTCTTCAACGCCTTCCGAAGGGT
GGATCACTGGCAGGTGCTTCACTTGTTCCCACGTTTTGTTTTGTTTTGTT
TTGTTTTTTTGTTTTTTTTCATGCTTTTTGGTTTTCTCGTCATTGGCCGG
TGGCAGGGTATGCCAGCGAGTGCCACGGGCATGTTTTTGTGTGTGTGTGT
GTTTTCAAGCATGACACTGAACCAGCTGTCACGAACGGTGTGTACTGGCG
TGTGCCTATCCAGAATGTACATGTGTACAAGTGCTCCCTGCGGAGTGGGC
TGATGGAAGCACAACAGTTTCACCGAGGGGTGATGCTAGATGAAAAAAAA
CAAAGCAGGCAGCAAGACTAACCCACAGCTAGCTGCCAGCTGCAGTTTGA
CGTTTCCCAATATCAACAGCTCACTCAGCTGAAGTGTTTTTGACTCGAAC
AGCCCAGAGAACCTCAACGAGCCAAAAACAAAAATTACAAAGTCTGTAGT
TAGTCTTGCAAGACGTACCAGGCTGCCAGACACACTAAATAGAGTTGGCG
TGGGTTTTCCCTGAAATACCAGCCCTACACGACGCCAGCATCCCCACCAT
GGACAACAACTTTTAACAACCACAACAACAACAACAACAACAACAACATC
AACATCAACATCAACAAAACCCTCTTACCGCAGGCGGCGGCCTCCACGCT
GCGCGAGATCCACGACTGGCCGGTCAAGCCGAACGTCTTCAACTACAACC
TCGCCATCTGCACCTGCACCGACTGCGGGGAGTGGGAGGAGGCCCTCGAC
CTGCTGCACTTCATGCGCGAGGTCGGCGGGGTGGAGCCGGACGTGGTGAC
GTACAACATCGTCGTGGCGGCCTGCGGGAAGGCCGGGCAGGGGGAGAAGG
CGATCGAGATATTCCGTGAGATGTCAGAGGTGGGTGACTGCTGCTGCTGC
TGTGTGTCGTTGAATCAGCATCGACCGCGCTAGTCGATGAAAGGATGTCA
GTCTTTGTAGCGAGATAAGAAACGCGAAATAAGAAACGCAAACACTATTC
GAAGGGTTTCTTTCACCGACAGAGGGGCGCAGGAGTTGGATTTGTGTGTA
TGTGTGTGTGCGTGTGTCTTTATATATTAGCATCGACCGCGCCAACCGGC
TGGCCAATCTTACAGACATCAGTCAGTCTTGCAGCGGTTGAAAAACAACA
CCTAGGTAAGTGTCAGCGAGTACTATGGTTTCGTTTGCTGATTTATCTAG
TAGAGTAGGAGACTTGTCTACTGCGGATGGAGATAGAAGTCGTTGTTCCT
TTATTGTAACGTAAAAAGACAACAAACGGCAGCAGCAGCAGCAGCGTAGC
GTTATCCAACACTGACAGCACCACCACCACAGGTCGGGATCAAGCCGGAC
GTGATCAGCTTCACCACGGCGATCTCCGCCTGCGGCAGCTGCGGTCTGTC
CGAGGAGGCCCTGTCCATCTTCCGCGAGATGGAGAGAGCCCGCGTGCGCC
CCAACATCATCACCCACAACGCGGTCATGTCCGCGTGCATCGCCGCCGGC
CAGTGGGAGGAGGCCCTCGACTTTTTCACCGAGGTGGTGGACGGCAGCGG
TGCCGCCAACGGCGGCGGCGGCGGCGGCGGTGGTGGTCCTCCGCGTGATC
CGGTAGAGGTGGCCGACGCGTGCAGCTACAACACGGCCCTATCGGCGTGC
GAGGTGAGGAAAAGCAGCAGCCGCCGCCGCCGCCGCCTTGATTGATTGGT
TGATTGGACCGCCTTCGCGATTAATAAACTTCTTGAGGAATAGAACGGGG
AGGGGAGGGTAGGGGGTACGCAGACCCTATCCGTAGCCTGAGCTCTCTCC
ATTGCTTTGTCTGTAGCGACGGCGACGCAGGTTGGAACGTGGCGTGTGGT
GTAGCGTATAAGGAGCTGGGCGGGGGGGGGGTGAGGGAAAGTTGCCAGGA
GCAGCGGCGGTGGCGATGATCCCCCAACCCCCCGGGTTTCCTTCTCAAAT
TGTTAACGTAGACCGTTGTCGTATGGTTGATTGCTCGTGTGTTGTAGCCA
AAAAAGAAGAAAGAATCGCGAGATCCTCTCCCAGTTGAACAGCCGGGGGT
GGTCTGTACACAGCGTTGTACGAAGAGCTCGAGAGAAGCGTGCTTGTCTG
CCGTTCGTATCTTCCAATCGATCATTCGTTGTTCATGGCAAGACATGACG
CAGAAAGCGAGAGCCCAGATGGATGTTTCACCCTTCCTCCCACCCCCCAC
CCGACACAGGTGGGCGAGCAGTGGGAGCGGGCGTTGTCTTTGTTGCGCGA
CATGCCCAAACGCGGCGTCGAGCCCGTTGTGATCAGCTACAACACGGTCA
TCTCGGCTTGCGGCAAGTGCGGAGAGGGTGACCAG
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Coding sequence (CDS) from alignment at Ecto-sp9_F_contig10381:1136..4570-

>mRNA_Ecto-sp9_F_contig10381.376.1 ID=mRNA_Ecto-sp9_F_contig10381.376.1|Name=mRNA_Ecto-sp9_F_contig10381.376.1|organism=Ectocarpus species9 EcSCA_722f female|type=CDS|length=2214bp|location=Sequence derived from alignment at Ecto-sp9_F_contig10381:1136..4570- (Ectocarpus species9 EcSCA_722f female)
GACGTCGCCGGCACTACCGCCGCCGTTCCCGTTAAAAAAAACAGGTACTG
CGTGAACGCTGCGCTGGACGCGTGCGGCAAGGCCGGGCGGGTCCAGGAAG
CCCTGGGCCTCATGAGGGACGCGCGGGCGTCGGGGATAGAGCTCGACGTT
GTGAGCTACAACTGCGCTATCCCGGCCTGCGTCACGGGGGGGGACTGGGA
GCTGGCCCTGAGCATGATTCGGGAGATGGAAGCCGAGTACGGCATCAAGC
CGAATCACATCACCTACCAGGCGGCGATAAAGGACGTCGCCGGCACTACC
GCCGCCGTTCCCGTTAAAAAAAACAGGTACTGCGTGAACGCTGCGCTGGA
CGCGTGCGGCAAGGCCGGGCGGGTCCAGGAAGCCCTGGGCCTCATGAGGG
ACGCGCGGGCGTCGGGGATAGAGCTCGACGTTGTGAGCTACAACTGCGCT
ATCCCGGCCTGCGTCACGGGGGGGGACTGGGAGCTGGCCCTGAGCATGAT
TCGGGAGATGGAAGCCGAGTACGGCATCAAGCCGAATCACATCACCTACC
AGGCGGCGATAAAGGTGTGCGGGAGGTGCGGCAAGGCTGACGAGGCGGCT
AGTCTCCTGAGAGAGATGAAGGACGGCGGCGTGCCGCTCAAGACGTCGGT
GTACTCGGCGGTCTTCAACGCCTTCCGAAGGGTGGATCACTGGCAGGTGT
GCGGGAGGTGCGGCAAGGCTGACGAGGCGGCTAGTCTCCTGAGAGAGATG
AAGGACGGCGGCGTGCCGCTCAAGACGTCGGTGTACTCGGCGGTCTTCAA
CGCCTTCCGAAGGGTGGATCACTGGCAGGCGGCGGCCTCCACGCTGCGCG
AGATCCACGACTGGCCGGTCAAGCCGAACGTCTTCAACTACAACCTCGCC
ATCTGCACCTGCACCGACTGCGGGGAGTGGGAGGAGGCCCTCGACCTGCT
GCACTTCATGCGCGAGGTCGGCGGGGTGGAGCCGGACGTGGTGACGTACA
ACATCGTCGTGGCGGCCTGCGGGAAGGCCGGGCAGGGGGAGAAGGCGATC
GAGATATTCCGTGAGATGTCAGAGGCGGCGGCCTCCACGCTGCGCGAGAT
CCACGACTGGCCGGTCAAGCCGAACGTCTTCAACTACAACCTCGCCATCT
GCACCTGCACCGACTGCGGGGAGTGGGAGGAGGCCCTCGACCTGCTGCAC
TTCATGCGCGAGGTCGGCGGGGTGGAGCCGGACGTGGTGACGTACAACAT
CGTCGTGGCGGCCTGCGGGAAGGCCGGGCAGGGGGAGAAGGCGATCGAGA
TATTCCGTGAGATGTCAGAGGTCGGGATCAAGCCGGACGTGATCAGCTTC
ACCACGGCGATCTCCGCCTGCGGCAGCTGCGGTCTGTCCGAGGAGGCCCT
GTCCATCTTCCGCGAGATGGAGAGAGCCCGCGTGCGCCCCAACATCATCA
CCCACAACGCGGTCATGTCCGCGTGCATCGCCGCCGGCCAGTGGGAGGAG
GCCCTCGACTTTTTCACCGAGGTGGTGGACGGCAGCGGTGCCGCCAACGG
CGGCGGCGGCGGCGGCGGTGGTGGTCCTCCGCGTGATCCGGTAGAGGTGG
CCGACGCGTGCAGCTACAACACGGCCCTATCGGCGTGCGAGGTCGGGATC
AAGCCGGACGTGATCAGCTTCACCACGGCGATCTCCGCCTGCGGCAGCTG
CGGTCTGTCCGAGGAGGCCCTGTCCATCTTCCGCGAGATGGAGAGAGCCC
GCGTGCGCCCCAACATCATCACCCACAACGCGGTCATGTCCGCGTGCATC
GCCGCCGGCCAGTGGGAGGAGGCCCTCGACTTTTTCACCGAGGTGGTGGA
CGGCAGCGGTGCCGCCAACGGCGGCGGCGGCGGCGGCGGTGGTGGTCCTC
CGCGTGATCCGGTAGAGGTGGCCGACGCGTGCAGCTACAACACGGCCCTA
TCGGCGTGCGAGGTGGGCGAGCAGTGGGAGCGGGCGTTGTCTTTGTTGCG
CGACATGCCCAAACGCGGCGTCGAGCCCGTTGTGATCAGCTACAACACGG
TCATCTCGGCTTGCGGCAAGTGCGGAGAGGGTGACCAGGTGGGCGAGCAG
TGGGAGCGGGCGTTGTCTTTGTTGCGCGACATGCCCAAACGCGGCGTCGA
GCCCGTTGTGATCAGCTACAACACGGTCATCTCGGCTTGCGGCAAGTGCG
GAGAGGGTGACCAG
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