mRNA_Ecto-sp8_S_contig99121.31766.1 (mRNA) Ectocarpus species8 EcLAC_412

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_Ecto-sp8_S_contig99121.31766.1
Unique NamemRNA_Ecto-sp8_S_contig99121.31766.1
TypemRNA
OrganismEctocarpus species8 EcLAC_412 (Ectocarpus species8 EcLAC_412)
Homology
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BLAST of mRNA_Ecto-sp8_S_contig99121.31766.1 vs. uniprot
Match: UPI000C786378 (eukaryotic translation initiation factor 2 subunit 2-like isoform X1 n=2 Tax=Eurytemora affinis TaxID=88015 RepID=UPI000C786378)

HSP 1 Score: 247 bits (630), Expect = 6.180e-79
Identity = 121/127 (95.28%), Postives = 123/127 (96.85%), Query Frame = 1 
Query:  175 WKDSDRDYQYDELLQRVFGIMRDKNPEMVAGEKKKFVMRPPQVARVGSKKTAFANFTEIAKMLHRQPKHLLAFLFAELGTSGAIDGSNQLIMKGRFQQKHIENVLRRYIKEYVTCHTCRSPDTILNK 555
            W DSDRDY Y+ELLQRVFGIMRDKNPEMVAGEKKKFVMRPPQV RVG+KKTAFANFTEIAKMLHR PKHLLAFLFAELGTSGAIDGSNQLIMKGRFQQKHIENVLRRYIKEYVTCHTCRSPDTILNK
Sbjct:  163 WVDSDRDYTYEELLQRVFGIMRDKNPEMVAGEKKKFVMRPPQVVRVGTKKTAFANFTEIAKMLHRTPKHLLAFLFAELGTSGAIDGSNQLIMKGRFQQKHIENVLRRYIKEYVTCHTCRSPDTILNK 289
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BLAST of mRNA_Ecto-sp8_S_contig99121.31766.1 vs. uniprot
Match: A0A553N8Y7_TIGCA (eIF2B_5 domain-containing protein n=1 Tax=Tigriopus californicus TaxID=6832 RepID=A0A553N8Y7_TIGCA)

HSP 1 Score: 240 bits (613), Expect = 1.250e-76
Identity = 116/128 (90.62%), Postives = 122/128 (95.31%), Query Frame = 1 
Query:  172 PWKDSDRDYQYDELLQRVFGIMRDKNPEMVAGEKKKFVMRPPQVARVGSKKTAFANFTEIAKMLHRQPKHLLAFLFAELGTSGAIDGSNQLIMKGRFQQKHIENVLRRYIKEYVTCHTCRSPDTILNK 555
            PW DSDRDY YDELLQRVF IMRDKNPE+VAGEKK+FVMRPPQV RVG+KKTAF NFTEIAKMLHRQPKHLLAFLFAELGT GAIDG+NQLI+KGRFQQKHIENVLRRYIKEYVTCHTCRSPDTIL+K
Sbjct:  144 PWVDSDRDYTYDELLQRVFNIMRDKNPELVAGEKKRFVMRPPQVVRVGTKKTAFVNFTEIAKMLHRQPKHLLAFLFAELGTVGAIDGNNQLIIKGRFQQKHIENVLRRYIKEYVTCHTCRSPDTILDK 271
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BLAST of mRNA_Ecto-sp8_S_contig99121.31766.1 vs. uniprot
Match: C1BN42_CALRO (Eukaryotic translation initiation factor 2 subunit 2 n=1 Tax=Caligus rogercresseyi TaxID=217165 RepID=C1BN42_CALRO)

HSP 1 Score: 236 bits (601), Expect = 2.030e-74
Identity = 115/128 (89.84%), Postives = 119/128 (92.97%), Query Frame = 1 
Query:  172 PWKDSDRDYQYDELLQRVFGIMRDKNPEMVAGEKKKFVMRPPQVARVGSKKTAFANFTEIAKMLHRQPKHLLAFLFAELGTSGAIDGSNQLIMKGRFQQKHIENVLRRYIKEYVTCHTCRSPDTILNK 555
            PW DSDRDY YDELLQRVF IMRDKNPE+VAGEKKKF+MRPPQV RVG+KKTAF NFTEIAKMLHRQ KHLL FL AELGT GAIDG+NQLIMKGRFQQKHIENVLRRYIKEYVTCHTCRSPDTILNK
Sbjct:  172 PWVDSDRDYTYDELLQRVFNIMRDKNPEVVAGEKKKFIMRPPQVVRVGTKKTAFVNFTEIAKMLHRQAKHLLDFLSAELGTVGAIDGNNQLIMKGRFQQKHIENVLRRYIKEYVTCHTCRSPDTILNK 299
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BLAST of mRNA_Ecto-sp8_S_contig99121.31766.1 vs. uniprot
Match: C1C345_CALCM (Eukaryotic translation initiation factor 2 subunit 2 n=1 Tax=Caligus clemensi TaxID=344056 RepID=C1C345_CALCM)

HSP 1 Score: 231 bits (590), Expect = 8.790e-73
Identity = 113/127 (88.98%), Postives = 118/127 (92.91%), Query Frame = 1 
Query:  175 WKDSDRDYQYDELLQRVFGIMRDKNPEMVAGEKKKFVMRPPQVARVGSKKTAFANFTEIAKMLHRQPKHLLAFLFAELGTSGAIDGSNQLIMKGRFQQKHIENVLRRYIKEYVTCHTCRSPDTILNK 555
            W DSDRDY YDELLQRVF IMRDKNPE+VAGEKK+F+MRPPQV RVG+KKTAF NFTEIAKMLHRQ KHLL FL AELGT GAIDG+NQLIMKGRFQQKHIENVLRRYIKEYVTCHTCRSPDTILNK
Sbjct:  171 WVDSDRDYTYDELLQRVFNIMRDKNPEVVAGEKKEFIMRPPQVVRVGTKKTAFVNFTEIAKMLHRQAKHLLDFLSAELGTVGAIDGNNQLIMKGRFQQKHIENVLRRYIKEYVTCHTCRSPDTILNK 297
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BLAST of mRNA_Ecto-sp8_S_contig99121.31766.1 vs. uniprot
Match: D3PHJ3_LEPSM (Eukaryotic translation initiation factor 2 subunit 2 n=5 Tax=Lepeophtheirus salmonis TaxID=72036 RepID=D3PHJ3_LEPSM)

HSP 1 Score: 229 bits (584), Expect = 5.760e-72
Identity = 111/128 (86.72%), Postives = 118/128 (92.19%), Query Frame = 1 
Query:  172 PWKDSDRDYQYDELLQRVFGIMRDKNPEMVAGEKKKFVMRPPQVARVGSKKTAFANFTEIAKMLHRQPKHLLAFLFAELGTSGAIDGSNQLIMKGRFQQKHIENVLRRYIKEYVTCHTCRSPDTILNK 555
            PW  SDRDY YDELLQRVF IM+DKNPE+VAGEKKKF+MRPPQV RVG+KKTAF NFTEIA+MLHRQ KHLL FL AELGT GAIDG+NQLIMKGRFQQKHIE+VLRRYIKEYVTCHTCRSPDTILNK
Sbjct:  163 PWMTSDRDYTYDELLQRVFNIMKDKNPEVVAGEKKKFIMRPPQVVRVGTKKTAFVNFTEIARMLHRQAKHLLDFLSAELGTVGAIDGNNQLIMKGRFQQKHIESVLRRYIKEYVTCHTCRSPDTILNK 290
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BLAST of mRNA_Ecto-sp8_S_contig99121.31766.1 vs. uniprot
Match: A0A7N5JV45_AILME (Eukaryotic translation initiation factor 2 subunit 2-like n=1 Tax=Ailuropoda melanoleuca TaxID=9646 RepID=A0A7N5JV45_AILME)

HSP 1 Score: 218 bits (555), Expect = 3.400e-69
Identity = 102/127 (80.31%), Postives = 116/127 (91.34%), Query Frame = 1 
Query:  175 WKDSDRDYQYDELLQRVFGIMRDKNPEMVAGEKKKFVMRPPQVARVGSKKTAFANFTEIAKMLHRQPKHLLAFLFAELGTSGAIDGSNQLIMKGRFQQKHIENVLRRYIKEYVTCHTCRSPDTILNK 555
            W  ++RDY YDELL RVF IMR+KNP+MVAGEK+KFVM+PPQV RVG+KKT+F NFT+I K+LHRQPKHLLAFL AELGTSG+IDG+NQL++KGRFQQK IENVLRRYIKEYVTCHTCRSPDTIL K
Sbjct:   43 WAGTERDYTYDELLNRVFNIMREKNPDMVAGEKRKFVMKPPQVVRVGTKKTSFVNFTDICKLLHRQPKHLLAFLLAELGTSGSIDGNNQLVIKGRFQQKQIENVLRRYIKEYVTCHTCRSPDTILQK 169
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BLAST of mRNA_Ecto-sp8_S_contig99121.31766.1 vs. uniprot
Match: Q4V876_XENLA (LOC780740 protein (Fragment) n=1 Tax=Xenopus laevis TaxID=8355 RepID=Q4V876_XENLA)

HSP 1 Score: 219 bits (558), Expect = 3.550e-69
Identity = 103/127 (81.10%), Postives = 116/127 (91.34%), Query Frame = 1 
Query:  175 WKDSDRDYQYDELLQRVFGIMRDKNPEMVAGEKKKFVMRPPQVARVGSKKTAFANFTEIAKMLHRQPKHLLAFLFAELGTSGAIDGSNQLIMKGRFQQKHIENVLRRYIKEYVTCHTCRSPDTILNK 555
            W  S+RDY YDELL RVF IMR+KNP+MVAGEK+KFVM+PPQV RVG+KKT+F NFT+I K+LHRQPKHLLAFL AELGTSG+IDG+NQL++KGRFQQK IENVLRRYIKEYVTCHTCRSPDTIL K
Sbjct:   77 WAGSERDYTYDELLNRVFNIMREKNPDMVAGEKRKFVMKPPQVVRVGTKKTSFVNFTDICKLLHRQPKHLLAFLLAELGTSGSIDGNNQLVIKGRFQQKQIENVLRRYIKEYVTCHTCRSPDTILQK 203
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BLAST of mRNA_Ecto-sp8_S_contig99121.31766.1 vs. uniprot
Match: A0A5A9NVE3_9TELE (Eukaryotic translation initiation factor 2 subunit 2 n=3 Tax=Cypriniformes TaxID=7952 RepID=A0A5A9NVE3_9TELE)

HSP 1 Score: 222 bits (565), Expect = 4.150e-69
Identity = 104/127 (81.89%), Postives = 117/127 (92.13%), Query Frame = 1 
Query:  175 WKDSDRDYQYDELLQRVFGIMRDKNPEMVAGEKKKFVMRPPQVARVGSKKTAFANFTEIAKMLHRQPKHLLAFLFAELGTSGAIDGSNQLIMKGRFQQKHIENVLRRYIKEYVTCHTCRSPDTILNK 555
            W DS+RDY YDELL RVF IMR+KNP+MVAGEK+KFVM+PPQV RVG+KKT+F NFT+I K+LHRQPKHLLAFL AELGTSG+IDG+NQL++KGRFQQK IENVLRRYIKEYVTCHTCRSPDTIL K
Sbjct:  162 WADSERDYTYDELLSRVFNIMREKNPDMVAGEKRKFVMKPPQVVRVGTKKTSFVNFTDICKLLHRQPKHLLAFLLAELGTSGSIDGNNQLVIKGRFQQKQIENVLRRYIKEYVTCHTCRSPDTILQK 288
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BLAST of mRNA_Ecto-sp8_S_contig99121.31766.1 vs. uniprot
Match: A0A3Q3X480_MOLML (Eukaryotic translation initiation factor 2, subunit 2 beta n=4 Tax=Euteleostomi TaxID=117571 RepID=A0A3Q3X480_MOLML)

HSP 1 Score: 218 bits (554), Expect = 5.500e-69
Identity = 102/127 (80.31%), Postives = 116/127 (91.34%), Query Frame = 1 
Query:  175 WKDSDRDYQYDELLQRVFGIMRDKNPEMVAGEKKKFVMRPPQVARVGSKKTAFANFTEIAKMLHRQPKHLLAFLFAELGTSGAIDGSNQLIMKGRFQQKHIENVLRRYIKEYVTCHTCRSPDTILNK 555
            W  S+RDY YDELL RVF IMR+KNP+MVAGEK+KFVM+PPQV RVG+KKT+F NFT+I K+LHRQPKHLLAFL AELGTSG+IDG+NQL++KGRFQQK IENVLRRYIKEYVTCHTCRSP+TIL K
Sbjct:   47 WAGSERDYTYDELLNRVFNIMREKNPDMVAGEKRKFVMKPPQVVRVGTKKTSFVNFTDICKLLHRQPKHLLAFLLAELGTSGSIDGNNQLVIKGRFQQKQIENVLRRYIKEYVTCHTCRSPETILQK 173
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BLAST of mRNA_Ecto-sp8_S_contig99121.31766.1 vs. uniprot
Match: A0A8C1RBY0_CYPCA (Eukaryotic translation initiation factor 2 subunit 2-like n=2 Tax=Cyprinus carpio TaxID=7962 RepID=A0A8C1RBY0_CYPCA)

HSP 1 Score: 217 bits (553), Expect = 5.640e-69
Identity = 102/127 (80.31%), Postives = 115/127 (90.55%), Query Frame = 1 
Query:  175 WKDSDRDYQYDELLQRVFGIMRDKNPEMVAGEKKKFVMRPPQVARVGSKKTAFANFTEIAKMLHRQPKHLLAFLFAELGTSGAIDGSNQLIMKGRFQQKHIENVLRRYIKEYVTCHTCRSPDTILNK 555
            W  S+RDY YDELL RVF IMR+KNP+MVAGEK+KFVM+PPQV RVG+KKT+F NFT+I K+LHRQPKHLLAFL AELGT G+IDG+NQL++KGRFQQK IENVLRRYIKEYVTCHTCRSPDTIL K
Sbjct:   37 WAGSERDYTYDELLSRVFNIMREKNPDMVAGEKRKFVMKPPQVVRVGTKKTSFVNFTDICKLLHRQPKHLLAFLLAELGTCGSIDGNNQLVIKGRFQQKQIENVLRRYIKEYVTCHTCRSPDTILQK 163
The following BLAST results are available for this feature:
BLAST of mRNA_Ecto-sp8_S_contig99121.31766.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
UPI000C7863786.180e-7995.28eukaryotic translation initiation factor 2 subunit... [more]
A0A553N8Y7_TIGCA1.250e-7690.63eIF2B_5 domain-containing protein n=1 Tax=Tigriopu... [more]
C1BN42_CALRO2.030e-7489.84Eukaryotic translation initiation factor 2 subunit... [more]
C1C345_CALCM8.790e-7388.98Eukaryotic translation initiation factor 2 subunit... [more]
D3PHJ3_LEPSM5.760e-7286.72Eukaryotic translation initiation factor 2 subunit... [more]
A0A7N5JV45_AILME3.400e-6980.31Eukaryotic translation initiation factor 2 subunit... [more]
Q4V876_XENLA3.550e-6981.10LOC780740 protein (Fragment) n=1 Tax=Xenopus laevi... [more]
A0A5A9NVE3_9TELE4.150e-6981.89Eukaryotic translation initiation factor 2 subunit... [more]
A0A3Q3X480_MOLML5.500e-6980.31Eukaryotic translation initiation factor 2, subuni... [more]
A0A8C1RBY0_CYPCA5.640e-6980.31Eukaryotic translation initiation factor 2 subunit... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
Ecto-sp8_S_contig99121contigEcto-sp8_S_contig99121:3..557 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Ectocarpus species8 EcLAC_412 OGS1.02022-07-08
Properties
Property NameValue
Taxonomic scopeMetazoa
Stop0
Start0
Seed ortholog score222.2
Seed ortholog evalue1.9e-55
Seed eggNOG ortholog128390.XP_009471540.1
Preferred nameEIF2S2
Model size555
KEGG koko:K03238
KEGG Pathwayko03013,map03013
Hectar predicted targeting categoryother localisation
GOsGO:0000003,GO:0000122,GO:0000902,GO:0000904,GO:0001701,GO:0001731,GO:0002176,GO:0002181,GO:0002183,GO:0003006,GO:0003674,GO:0003676,GO:0003723,GO:0003743,GO:0005488,GO:0005575,GO:0005622,GO:0005623,GO:0005737,GO:0005829,GO:0005850,GO:0006355,GO:0006357,GO:0006412,GO:0006413,GO:0006518,GO:0006807,GO:0006810,GO:0006928,GO:0006935,GO:0006950,GO:0007275,GO:0007276,GO:0007399,GO:0007409,GO:0007411,GO:0007548,GO:0007568,GO:0008037,GO:0008038,GO:0008135,GO:0008150,GO:0008152,GO:0008283,GO:0008340,GO:0008406,GO:0008584,GO:0009058,GO:0009059,GO:0009605,GO:0009653,GO:0009790,GO:0009792,GO:0009889,GO:0009890,GO:0009892,GO:0009987,GO:0010259,GO:0010467,GO:0010468,GO:0010556,GO:0010558,GO:0010605,GO:0010629,GO:0016043,GO:0016198,GO:0016199,GO:0016282,GO:0019219,GO:0019222,GO:0019538,GO:0019953,GO:0022008,GO:0022414,GO:0022607,GO:0022613,GO:0022618,GO:0030030,GO:0030154,GO:0030182,GO:0031175,GO:0031323,GO:0031324,GO:0031326,GO:0031327,GO:0032501,GO:0032502,GO:0032504,GO:0032989,GO:0032990,GO:0032991,GO:0033554,GO:0034622,GO:0034641,GO:0034645,GO:0034976,GO:0036093,GO:0040008,GO:0040011,GO:0040014,GO:0042221,GO:0042330,GO:0043009,GO:0043043,GO:0043170,GO:0043603,GO:0043604,GO:0043614,GO:0043933,GO:0044085,GO:0044237,GO:0044238,GO:0044249,GO:0044260,GO:0044267,GO:0044271,GO:0044424,GO:0044444,GO:0044464,GO:0044703,GO:0045137,GO:0045892,GO:0045934,GO:0046546,GO:0046661,GO:0048468,GO:0048513,GO:0048519,GO:0048523,GO:0048608,GO:0048609,GO:0048638,GO:0048666,GO:0048667,GO:0048699,GO:0048731,GO:0048812,GO:0048856,GO:0048858,GO:0048869,GO:0050789,GO:0050793,GO:0050794,GO:0050896,GO:0051171,GO:0051172,GO:0051179,GO:0051234,GO:0051239,GO:0051252,GO:0051253,GO:0051704,GO:0051716,GO:0055085,GO:0060255,GO:0061458,GO:0061564,GO:0065003,GO:0065007,GO:0070993,GO:0071704,GO:0071826,GO:0071840,GO:0080090,GO:0097159,GO:0097485,GO:0120036,GO:0120039,GO:1901363,GO:1901564,GO:1901566,GO:1901576,GO:1902679,GO:1903506,GO:1903507,GO:1990904,GO:2000112,GO:2000113,GO:2001141
Exons1
EggNOG free text desc.translation initiation factor activity
EggNOG OGs38MQN@33154,3BD1P@33208,3CUV4@33213,4842Z@7711,48USV@7742,4GJWC@8782,COG1601@1,KOG2768@2759
Cds size555
COG Functional cat.J
Best tax levelAves
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko01009,ko03012
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1681145360.223345-CDS-Ecto-sp8_S_contig99121:2..5571681145360.223345-CDS-Ecto-sp8_S_contig99121:2..557Ectocarpus species8 EcLAC_412CDSEcto-sp8_S_contig99121 3..557 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_Ecto-sp8_S_contig99121.31766.1prot_Ecto-sp8_S_contig99121.31766.1Ectocarpus species8 EcLAC_412polypeptideEcto-sp8_S_contig99121 3..557 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_Ecto-sp8_S_contig99121.31766.1

>prot_Ecto-sp8_S_contig99121.31766.1 ID=prot_Ecto-sp8_S_contig99121.31766.1|Name=mRNA_Ecto-sp8_S_contig99121.31766.1|organism=Ectocarpus species8 EcLAC_412|type=polypeptide|length=185bp
VKDALPEDENKAEEEEDMDLDLDSFGTKKKKKKKKKADLDDFDNEEDKEN
EDDPNTDPWKDSDRDYQYDELLQRVFGIMRDKNPEMVAGEKKKFVMRPPQ
VARVGSKKTAFANFTEIAKMLHRQPKHLLAFLFAELGTSGAIDGSNQLIM
KGRFQQKHIENVLRRYIKEYVTCHTCRSPDTILNK
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mRNA from alignment at Ecto-sp8_S_contig99121:3..557-

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_Ecto-sp8_S_contig99121.31766.1 ID=mRNA_Ecto-sp8_S_contig99121.31766.1|Name=mRNA_Ecto-sp8_S_contig99121.31766.1|organism=Ectocarpus species8 EcLAC_412|type=mRNA|length=555bp|location=Sequence derived from alignment at Ecto-sp8_S_contig99121:3..557- (Ectocarpus species8 EcLAC_412)
GTGAAGGATGCCCTCCCTGAAGATGAGAACAAAGCTGAAGAAGAGGAGGA
TATGGACCTGGATTTGGACAGCTTTGGAACCAAGAAAAAGAAGAAGAAAA
AGAAGAAAGCAGACCTTGATGATTTCGACAATGAAGAGGACAAAGAGAAC
GAAGACGACCCCAACACTGACCCCTGGAAAGATTCTGATCGTGACTACCA
GTACGACGAGTTGCTTCAGAGAGTGTTTGGCATCATGAGGGACAAGAACC
CCGAGATGGTCGCTGGAGAGAAGAAGAAGTTCGTGATGAGACCCCCACAA
GTTGCCAGGGTTGGCAGTAAGAAAACTGCGTTTGCTAACTTCACTGAGAT
TGCCAAGATGCTCCACAGACAGCCCAAGCACTTGTTGGCCTTTCTCTTTG
CTGAGTTGGGAACCAGTGGGGCTATTGATGGCAGCAATCAACTCATCATG
AAAGGTCGTTTCCAACAGAAGCACATAGAGAATGTGTTGAGGAGATATAT
CAAGGAGTATGTCACCTGCCACACATGCAGATCCCCGGACACCATCCTGA
ACAAG
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Coding sequence (CDS) from alignment at Ecto-sp8_S_contig99121:3..557-

>mRNA_Ecto-sp8_S_contig99121.31766.1 ID=mRNA_Ecto-sp8_S_contig99121.31766.1|Name=mRNA_Ecto-sp8_S_contig99121.31766.1|organism=Ectocarpus species8 EcLAC_412|type=CDS|length=555bp|location=Sequence derived from alignment at Ecto-sp8_S_contig99121:3..557- (Ectocarpus species8 EcLAC_412)
GTGAAGGATGCCCTCCCTGAAGATGAGAACAAAGCTGAAGAAGAGGAGGA
TATGGACCTGGATTTGGACAGCTTTGGAACCAAGAAAAAGAAGAAGAAAA
AGAAGAAAGCAGACCTTGATGATTTCGACAATGAAGAGGACAAAGAGAAC
GAAGACGACCCCAACACTGACCCCTGGAAAGATTCTGATCGTGACTACCA
GTACGACGAGTTGCTTCAGAGAGTGTTTGGCATCATGAGGGACAAGAACC
CCGAGATGGTCGCTGGAGAGAAGAAGAAGTTCGTGATGAGACCCCCACAA
GTTGCCAGGGTTGGCAGTAAGAAAACTGCGTTTGCTAACTTCACTGAGAT
TGCCAAGATGCTCCACAGACAGCCCAAGCACTTGTTGGCCTTTCTCTTTG
CTGAGTTGGGAACCAGTGGGGCTATTGATGGCAGCAATCAACTCATCATG
AAAGGTCGTTTCCAACAGAAGCACATAGAGAATGTGTTGAGGAGATATAT
CAAGGAGTATGTCACCTGCCACACATGCAGATCCCCGGACACCATCCTGA
ACAAG
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