mRNA_Ecto-sp8_S_contig9740.31478.1 (mRNA) Ectocarpus species8 EcLAC_412

BLAST of mRNA_Ecto-sp8_S_contig9740.31478.1 vs. uniprot
Match: A0A6H5JKA8_9PHAE (Subtilisin n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JKA8_9PHAE)

HSP 1 Score: 831 bits (2146), Expect = 1.090e-288
Identity = 426/490 (86.94%), Postives = 439/490 (89.59%), Query Frame = 3

Query:  651 DNISGGPSGVTGLTVELAPGVLAATNRRGSDGGGWYASSGELTDEWREELMSESLDVHGKSFWSSGWSAKLHDEAAAEGAGDSSGGGPGEVLAREWTRAADVLRGLGGTEGGPTIGDVCSWDGLVVVHTDDDLLTMKGNSALLVQ---LGNLFYPCADELQVACFMALLSFLASRPEVLRVSPARAIKLLNAAAYHNVQSATLTESPMTDAGLDGTGEVIQIVDTGLDVNSCFFVDEDGGEGVAHGHYFEEIGVEASALSSWFTSWDFNLQIARVFTGGDFNFDLGRRK---------------IVQYINL--IRQDSETNS--TANNSTNYGESWATSAGEQYPWISAAEFAEDYAGGHGTHTAGSAAGAVVSSPSYSQTGDFTEVCEEGRELSCVGGCIDVDDTFGGDDLITTWDYQYFWAGYEAIDLDRLCPRLDCDDLPEEYCLGDDEAETLQQHGGVARGAQLAIFDILDDYTGIGIEMAGNGMW 2054
            DNI GGPSGVTGLTVELAPGVLAATNRRGS GGGWYASSGELTDEWREELMS+SLDVH KSFWSSGWSAKL DEAAAEGAGDSSGGGPGEVLAREWTRAADVLRGLGGTEGGPTIGDVCSWDGLVVVHTDDDLLTMKG   LL +    G    P ADEL+VACFMALLSFLASRPEVLRVSPARAIKLLNAAAYHNVQSA+LTESPMTDAGLDGTGEVIQIVDTGLDVNSCFFVDEDGGEGVAHG+YFEEIGVEAS+  SWFTSWDFN+QIARVFTGGDFNFDL RRK               I +   L  +R  S  N   TANNSTNYGESWATSAGEQYPWISAAEFAED AGGHGTHTAGSAAGAVVSSPSYSQTGDFTEVCEEGRELSCVGGCIDVDD FG DDLITTWDYQYFWAGYEAIDLDRLCPRLDCDDLPEEYCLGDDEAETLQQHGGVARGAQLAIFDI++DYTG+GI+MAGNGMW
Sbjct:  215 DNIGGGPSGVTGLTVELAPGVLAATNRRGSSGGGWYASSGELTDEWREELMSQSLDVHDKSFWSSGWSAKLPDEAAAEGAGDSSGGGPGEVLAREWTRAADVLRGLGGTEGGPTIGDVCSWDGLVVVHTDDDLLTMKGMDHLLPEGRGAGGNEGPEADELKVACFMALLSFLASRPEVLRVSPARAIKLLNAAAYHNVQSASLTESPMTDAGLDGTGEVIQIVDTGLDVNSCFFVDEDGGEGVAHGYYFEEIGVEASSFGSWFTSWDFNVQIARVFTGGDFNFDLSRRKGLVVDAAHPSCDSPNISRRFALGDVRAWSGENPACTANNSTNYGESWATSAGEQYPWISAAEFAEDDAGGHGTHTAGSAAGAVVSSPSYSQTGDFTEVCEEGRELSCVGGCIDVDDPFGEDDLITTWDYQYFWAGYEAIDLDRLCPRLDCDDLPEEYCLGDDEAETLQQHGGVARGAQLAIFDIINDYTGVGIDMAGNGMW 704          

BLAST of mRNA_Ecto-sp8_S_contig9740.31478.1 vs. uniprot
Match: D8LBZ2_ECTSI (Subtilisin (Fragment) n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LBZ2_ECTSI)

HSP 1 Score: 793 bits (2048), Expect = 1.780e-281
Identity = 398/421 (94.54%), Postives = 401/421 (95.25%), Query Frame = 3

Query:  801 MSESLDVHGKSFWSSGWSAKLHDEAAAEGAGDSSGGGPGEVLAREWTRAADVLRGLGGTEGGPTIGDVCSWDGLVVVHTDDDLLTMKGNSALLVQ---LGNLFYPCADELQVACFMALLSFLASRPEVLRVSPARAIKLLNAAAYHNVQSATLTESPMTDAGLDGTGEVIQIVDTGLDVNSCFFVDEDGGEGVAHGHYFEEIGVEASALSSWFTSWDFNLQIARVFTGGDFNFDLGRRKIVQYINLIRQDSETNSTANNSTNYGESWATSAGEQYPWISAAEFAEDYAGGHGTHTAGSAAGAVVSSPSYSQTGDFTEVCEEGRELSCVGGCIDVDDTFGGDDLITTWDYQYFWAGYEAIDLDRLCPRLDCDDLPEEYCLGDDEAETLQQHGGVARGAQLAIFDILDDYTGIGIEMAGNGMW 2054
            MSESLDVHGKSFWSSGWSAKL DEAAAEGAGDS GGGPGEVLAREWTRAADVLRGLGG EGGPTIGDVCSWDGLVVVHTDDDLLTMKG   LL +    G    P ADELQVACFMALLSFLASRPEVLRVSPARAIKLLNAAAYHNVQSA LTESPMTDAGLDGTGEVIQIVDTGLDVNSCFFVDEDGGEGVAHGHYFEEIGVEASALSSWFTSWDFNLQIARVFTGGDFNFDL RRKIVQYINLIRQDSETNSTANNSTNYGESWATSAG+QYPWISA EFAE+YAGGHGTHTAGSAAGAVVSSPSYSQTGDFTEVCEEGRELSCVGGCIDVDDTFGGDDLITTWDYQYFWAGYEAIDLDRLCPRLDCDD PEEYCLGDDEAETLQQHGGVARGAQLAIFDILDDYTGIGIEMAGNGMW
Sbjct:    1 MSESLDVHGKSFWSSGWSAKLLDEAAAEGAGDSLGGGPGEVLAREWTRAADVLRGLGGAEGGPTIGDVCSWDGLVVVHTDDDLLTMKGMDHLLPEGRGAGGNEGPEADELQVACFMALLSFLASRPEVLRVSPARAIKLLNAAAYHNVQSANLTESPMTDAGLDGTGEVIQIVDTGLDVNSCFFVDEDGGEGVAHGHYFEEIGVEASALSSWFTSWDFNLQIARVFTGGDFNFDLSRRKIVQYINLIRQDSETNSTANNSTNYGESWATSAGDQYPWISAPEFAENYAGGHGTHTAGSAAGAVVSSPSYSQTGDFTEVCEEGRELSCVGGCIDVDDTFGGDDLITTWDYQYFWAGYEAIDLDRLCPRLDCDDRPEEYCLGDDEAETLQQHGGVARGAQLAIFDILDDYTGIGIEMAGNGMW 421          

BLAST of mRNA_Ecto-sp8_S_contig9740.31478.1 vs. uniprot
Match: D7G6L6_ECTSI (Subtilisin n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7G6L6_ECTSI)

HSP 1 Score: 506 bits (1303), Expect = 1.310e-173
Identity = 279/410 (68.05%), Postives = 280/410 (68.29%), Query Frame = 3

Query:  801 MSESLDVHGKSFWSSGWSAKLHDEAAAEGAGDSSGGGPGEVLAREWTRAADVLRGLGGTEGGPTIGDVCSWDGLVVVHTDDDLLTMKGNSALLVQ---LGNLFYPCADELQVACFMALLSFLASRPEVLRVSPARAIKLLNAAAYHNVQSATLTESPMTDAGLDGTGEVIQIVDTGLDVNSCFFVDEDGGEGVAHGHYFEEIGVEASALSSWFTSWDFNLQIARVFTGGDFNFDLGRRKIVQYINLIRQDSETNSTANNSTNYGESWATSAGEQYPWISAAEFAEDYAGGHGTHTAGSAAGAVVSSPSYSQTGDFTEVCEEGRELSCVGGCIDVDDTFGGDDLITTWDYQYFWAGYEAIDLDRLCPRLDCDDLPEEYCLGDDEAETLQQHGGVARGAQLAIFDILDDYTG 2021
            MSESLDVHGKSFWSSGWSAKL DEAAAEGAGDS GGGPGEVLAREWTRAADVLRGLGG EGGPTIGDVCSWDGLVVVHTDDDLLTMKG   LL +    G    P ADELQVACFMALLSFLASRPEVLRVSPARAIKLLNAAAYHNVQSA LTESPMTDAGLDGTGEVIQIVDTGLDVNSCFFVDEDGGEGVAHGHYFEEIGVEASALSSWFTSWDFNLQIARVFTGGDFNFDL RRK                                                                                                               YFWAGYEAIDLDRLCPRLDCDD PEEYCLGDDEAETLQQHGGVARGAQLAIFDILDDYTG
Sbjct:    1 MSESLDVHGKSFWSSGWSAKLLDEAAAEGAGDSLGGGPGEVLAREWTRAADVLRGLGGAEGGPTIGDVCSWDGLVVVHTDDDLLTMKGMDHLLPEGRGAGGNEGPEADELQVACFMALLSFLASRPEVLRVSPARAIKLLNAAAYHNVQSANLTESPMTDAGLDGTGEVIQIVDTGLDVNSCFFVDEDGGEGVAHGHYFEEIGVEASALSSWFTSWDFNLQIARVFTGGDFNFDLSRRK---------------------------------------------------------------------------------------------------------------YFWAGYEAIDLDRLCPRLDCDDRPEEYCLGDDEAETLQQHGGVARGAQLAIFDILDDYTG 299          

BLAST of mRNA_Ecto-sp8_S_contig9740.31478.1 vs. uniprot
Match: A0A6H5JQT4_9PHAE (Subtilisin n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JQT4_9PHAE)

HSP 1 Score: 323 bits (828), Expect = 5.940e-94
Identity = 203/488 (41.60%), Postives = 275/488 (56.35%), Query Frame = 3

Query:  672 SGVTGLTVELAPGVLAATNRRGSDGGGWYASSGELTDEWREELMSESLDVHGKSFWSSGWSAKLHDEAAAEGAGDSSGGGPGEVLAREWTRAADVLRGLGGTEGGPTIGDVCSWDGLVVVHTDDDLLTMKGNSALLVQLGNLFYPCAD-----ELQVACFMALLSFLASRPEVLRVSPARAIKLLNAAAYHNVQSATLTESPMTDAGLDGTGEVIQIVDTGLDVNSCFFVDEDGGEGVAHGHYFEEIGVEASALSSWFTS----------WD---------FNLQIARVFTGGDFNFDLGRRKIVQYINLIRQDSETNSTANNSTNYGESWATSAGEQYPWISAAEFAEDYAGGHGTHTAGSAAGAVVSSPSYSQTGDFTEVCEEGRELSCVGGCIDVDDTFGGDDLITTWDYQYFWAGYEAIDLDRLCPRL---DCDDLPEEYCLGDDEAETLQQHGGVARGAQLAIFDILDDYTGIGIEMAGNGMW 2054
            S V GL++EL PG LA+ +            +     +   +LM  S+D+H  +FWS        D+    G G+      G V AR+W++AA V+  L     G + GD+CSW+ + V H + D+L + G   LL   G      +D     EL +ACFM L+SFLAS+PEVLRV+P ++  LLNA+A  N+QSAT+T++P+TDAGLDGTG+V+Q++D+G+D +SCFF  ++ G+ V HGHYFEE G+     SS F+S          W             QIA  F GGDF     RRKI+QYINLI+ D    S       +G+S+ TS G+QYPW+ A  F  DY  GHGTH AGS AGA +++P+   +      C++   +SCVG C+D D+    DDL T           E +DLDRLCP     DCD L  E CL DD AETL  +GG+A+GA+LAIFD   D  G+     GNG+W
Sbjct:  174 SNVEGLSIELTPGTLASRS----------VEAEAFISDTLGDLMLASVDLHSTNFWS--------DQNM--GGGEQLSFPGGAVRARDWSKAASVVHELS-EAAGTSPGDICSWNRVSVHHAEGDVLLVSGLDHLLHS-GRGAGGRSDGGESAELHIACFMGLVSFLASKPEVLRVAPRQSQALLNASARANIQSATVTDTPLTDAGLDGTGQVVQVIDSGVDESSCFFAHDESGDHVPHGHYFEEWGINPPLTSSSFSSPTSDDDDSLGWPRATDDHRTGSYYQIAEEFDGGDFTIFPDRRKIIQYINLIKSDDTEGS-------HGDSFTTSLGQQYPWLPADRFEYDYEAGHGTHVAGSVAGATLNTPAELIS------CKDTETMSCVGACVDDDEANDVDDLAT--------PNVEIVDLDRLCPLFSDYDCDVLETEKCLSDDVAETLTNNGGMAQGAKLAIFDAFYDNFGLS-PFVGNGLW 617          

BLAST of mRNA_Ecto-sp8_S_contig9740.31478.1 vs. uniprot
Match: A0A6H5K229_9PHAE (Subtilisin n=2 Tax=Ectocarpus TaxID=2879 RepID=A0A6H5K229_9PHAE)

HSP 1 Score: 296 bits (759), Expect = 1.150e-84
Identity = 196/465 (42.15%), Postives = 265/465 (56.99%), Query Frame = 3

Query:  672 SGVTGLTVELAPGVLAATNRRGSDGGGWYASSGELTDEWREELMSESLDVHGKSFWSSGWSAKLHDEAAAEGAGDSSGGGPGEVLAREWTRAADVLRGLGGTEGGPTIGDVCSWDGLVVVHTDDDLLTMKGNSALLVQ---LGNLFYPCADELQVACFMALLSFLASRPEVLRVSPARAIKLLNAAAYHNVQSATLTESPMTDAGLDGTGEVIQIVDTGLDVNSCFFVDEDGGEGVAHGHYFEEIGVEASALSSWFTSWDFNLQIARVFTGGDFNFDLGRRKIVQYINLIRQDSETNSTANNSTNYGESWATSAGEQYPWISAAEFAEDYAGGHGTHTAGSAAGAVVSSPSYSQTGDFTEVCEEGRELSCVGGCIDVDDTFGGDDLITTWDYQYFWAGYEAI-DLDRLCPRLDCDDLPEEYCLGDDEAETLQQHGGVARGAQLAIFDILDDYTGIGIEMAGNGMW 2054
            S V GL V+L P  L A +          A +     +   +LMS S+D+H  +FWS        D   A G   SS GG   V AR+W++AA V+  L     G + GD+CSWD + ++   DD L + G   LL      G      + EL VACFM L+SF A RPEVLRVSP    ++LN AA   +QSA+ T++P+TDAGLDGTGEVIQ+VDTGLD  SCFF+D DGGE V HG++FEE+ V ++  S         L    VF  GDF +   RRK++QYI L+  DS+             +++T+ G  YP++   EF +D + GHGTHTAGSAAGA ++ P+ + T      C     L CVGGCID  +T   DDL++       +A   A+ DLDRLCP   CD   +  CL D+  ETL ++GG+A+GA+L+IFD+  +  G+ IE+ GNG+W
Sbjct:  184 SNVEGLNVKLTPDTLPARS----------AGAESFIIDLLGDLMSASVDLHSTNFWS--------DPVMAGGEHLSSHGGA--VRARDWSKAASVVHELS-EAAGTSPGDICSWDEVALIQAGDDSLLVTGLDHLLFSGRGAGGSHEEESTELHVACFMGLVSFFAGRPEVLRVSPRHTKRVLNEAARGIIQSASATDTPLTDAGLDGTGEVIQVVDTGLDETSCFFID-DGGEEVDHGYFFEELFVGSTGSSQ--------LIYYPVFEAGDFTYTEERRKVIQYIELVNPDSDY-----------VAFSTTQGGVYPFLPPDEFGQDDSAGHGTHTAGSAAGATLNDPAETLT------CSGDETLGCVGGCIDESETT--DDLVS-------YAIQIALPDLDRLCPMYGCDGSTDP-CLSDEVDETLTKNGGMAQGAKLSIFDVFFEVFGL-IEVLGNGVW 590          

BLAST of mRNA_Ecto-sp8_S_contig9740.31478.1 vs. uniprot
Match: D8LNG4_ECTSI (Subtilisin n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LNG4_ECTSI)

HSP 1 Score: 262 bits (670), Expect = 1.040e-73
Identity = 154/329 (46.81%), Postives = 201/329 (61.09%), Query Frame = 3

Query: 1137 MALLSFLASRPEVLRVSPARAIKLLNAAAYHNVQSATLTESPMTDAGLDGTGEVIQIVDTGLDVNSCFFVDEDGGEGVAHGHYFEEIGVEASALSSWFTS-------------W---DFNL----QIARVFTGGDFNFDLGRRKIVQYINLIRQDSETNSTANNSTNYGESWATSAGEQYPWISAAEFAEDYAGGHGTHTAGSAAGAVVSSPSYSQTGDFTEVCEEGRELSCVGGCIDVDDTFGGDDLITTWDYQYFWAGYEAIDLDRLCPRL---DCDDLPEEYCLGDDEAETLQQHGGVARGAQLAIFDILDDYTGIGIEMAGNGMW 2054
            M L+SFLAS+PEVLRV+P ++  LLNAAA  N+QSAT+T++P+TDAGLDGTG+V+Q++D+G+D +SCFF  ++ G+ V HGHYFEE G+  S  SS F+S             W   D +     QIA  F GGDF     RRKI+QYINLI+ D    S       +G+S+ TS G+QYPW+SA  F  D   GHGTH AGS AGA +++P+   +      C +   +SCVG C+D D+    DDL T +         E  DLDRLCP     DCD L  + CL DD AETL  +GG+A+GA+LAIFD   D  G+     G G+W
Sbjct:    1 MGLVSFLASKPEVLRVAPRQSQALLNAAARANIQSATVTDTPLTDAGLDGTGQVVQVIDSGVDESSCFFAHDESGDHVPHGHYFEEWGINPSLTSSSFSSPXXXXXXXXXXXPWATADHHTGSYYQIAEEFDGGDFTIYPDRRKIIQYINLIKSDDTEGS-------HGDSFTTSLGQQYPWLSADHFEYDDEAGHGTHVAGSVAGATLNTPAELIS------CNDTETMSCVGACVDDDEANDVDDLATPY--------VEIADLDRLCPLFSDYDCDGLETDKCLSDDVAETLTNNGGMAQGAKLAIFDAFYDTFGLS-PFVGTGLW 307          

BLAST of mRNA_Ecto-sp8_S_contig9740.31478.1 vs. uniprot
Match: D8LNG6_ECTSI (Subtilisin n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LNG6_ECTSI)

HSP 1 Score: 266 bits (679), Expect = 1.330e-73
Identity = 180/464 (38.79%), Postives = 242/464 (52.16%), Query Frame = 3

Query:  678 VTGLTVELAPGVLAATNRRGSDGGGWYASSGELTDEWREELMSESLDVHGKSFWSSGWSAKLHDEAAAEGAGDSSGGGPGEVLAREWTRAADVLRGLG-GTEGGPTIGDVCSWDGLVVVHTDDDLLTMKGNSALLVQ---LGNLFYPCADELQVACFMALLSFLASRPEVLRVSPARAIKLLNAAAYHNVQSATLTESPMTDAGLDGTGEVIQIVDTGLDVNSCFFVDEDGGEGVAHGHYFEEIGVEASALSSWFTSWDFNLQIARVFTGGDFNFDLGRRKIVQYINLIRQDSETNSTANNSTNYGESWATSAGEQYPWISAAEFAEDYAGGHGTHTAGSAAGAVVSSPSYSQTGDFTEVCEEGRELSCVGGCIDVDDTFGGDDLITTWDYQYFWAGYEAIDLDRLCPRLDCDDLPEEYCLGDDEAETLQQHGGVARGAQLAIFDILDDYTGIGIEMA-GNGMW 2054
            V GL+VEL PG LAA +          + +    +    +LMS S+D+H  +FWS         +   +GAG+      G V AR+W +AA V+  L      GP  GD+CSW+ + V H  DD L + G   LL      G      + EL VACFM L+SFLA RPEVLRVSP    ++LNAAA   +QSA+ T++P+TDAGLDGTGE+IQ++D+GLD  SC+F+D D GE V HG+YF+E+G  A   SS+ +      + A +F GGDF++   RRK                                                  GHGTHTAGSAAGA ++ P+ + T + T+       LSCVG CID+ D    DDL++           +  DLDRLCP   CD+  +  CL DD  ETL  +GG+A+GA+LAIFD+   Y G     + GN +W
Sbjct:  183 VEGLSVELTPGTLAARS----------SEAKSFINHMLGDLMSASVDLHSTNFWS---------DPGMDGAGEHLSSPAGAVRARDWRKAASVVHELSEAASTGP--GDICSWNRIFVHHAGDDSLLVSGLDHLLFSGRGAGGSHEEESTELHVACFMGLVSFLAGRPEVLRVSPRPTKRVLNAAARGVIQSASATDTPLTDAGLDGTGEIIQVIDSGLDETSCYFID-DSGEEVDHGYYFDELGRAAVYSSSYSSD-----ETATIFNGGDFSYYPERRKA-------------------------------------------------GHGTHTAGSAAGATLNDPAETITCNVTDT------LSCVGACIDLLDAT--DDLVSISSQT------DGTDLDRLCPMYGCDNSTDTTCLSDDVPETLSNNGGMAQGAKLAIFDVF--YGGYDASSSIGNNLW 554          

BLAST of mRNA_Ecto-sp8_S_contig9740.31478.1 vs. uniprot
Match: A0A2I6U1R2_SACJA (Subtilisin (Fragment) n=1 Tax=Saccharina japonica TaxID=88149 RepID=A0A2I6U1R2_SACJA)

HSP 1 Score: 260 bits (665), Expect = 3.670e-72
Identity = 176/461 (38.18%), Postives = 235/461 (50.98%), Query Frame = 3

Query:  678 VTGLTVELAPGVLAATNRRGSDGGGWYASSGELTDEWREELMSESLDVHGKSFWSSGWSAKLHDEAAAEGAGDSSGGGPGEVLAREWTRAADVLRGLGGTEGGPTIGDVCSWDGLVVVHTDDDLLTMKGNSALLVQLGNLFYPC--ADELQVACFMALLSFLASRPEVLRVSPARAIKLLNAAAYHNVQSATLTESPMTDAGLDGTGEVIQIVDTGLDVNSCFFVDEDGGEGVAHGHYFEEIGVEASALSSWFTSWDFNLQIARVFTGGDFNFDLGRRKIVQYINLIRQDSETNSTANNSTNYGESWATSAGEQYPWISAAEFAEDYAGGHGTHTAGSAAGAVVSSPSYSQTGDFTEVCEEGRELSCVGGCIDVDDTFGGDDLITTWDYQYFWAGYEAIDLDRLCPRLDCDDLPEEYCLGDDEAETLQQHGGVARGAQLAIFDILDDYTGIGIEMAGNGMW 2054
            V GL +EL+PG L A +            +G    E  ++LMS+S+D+HG + WS        D  AA G   ++  G   V  REW+ AA ++  L    G  + GD+CSWD + + +  +DLL + G   LL +          A EL+VACFM L+SFL++R EVLRVSP  A +LLNA A   +QSAT+TE+P+TDAGLDGTG+VIQ++DTGLD  SCFF+D+DG E V HGHY+E +G                                   +I+   +  R D E                                     GHGTH AGSA GA + +P+ + T      C       CVGGCID D T  GDDL T       ++ Y+ ID+DRLCP +DCDD  +  CL DD  +TL  HGG+A+GA+LA FD+  D +  G    GNG+W
Sbjct:  183 VEGLNMELSPGTLPAHS----------PEAGMFIAELLDDLMSDSIDLHGGNVWS--------DPVAANGEHLTTPAGA--VRKREWSMAATLVHQLA-VAGRTSPGDICSWDSISMHYAGNDLLLVSGLDHLLHKENGAIGSDEEATELRVACFMGLVSFLSNRLEVLRVSPRHADRLLNAVARTIIQSATITEAPLTDAGLDGTGQVIQVIDTGLDETSCFFIDDDGEE-VPHGHYYEALG-----------------------------------EIIPSSSSARDDVE-------------------------------------GHGTHCAGSALGATIRTPAETPT-----TCSGTEVPGCVGGCIDADRTSWGDDLFTLMPE---YSPYD-IDIDRLCPMIDCDDATDPRCLSDDVGQTLADHGGMAQGAKLAFFDVFADGSS-GTHFVGNGLW 539          

BLAST of mRNA_Ecto-sp8_S_contig9740.31478.1 vs. uniprot
Match: D8LSW0_ECTSI (Subtilisin n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LSW0_ECTSI)

HSP 1 Score: 235 bits (599), Expect = 1.890e-62
Identity = 184/513 (35.87%), Postives = 243/513 (47.37%), Query Frame = 3

Query:  678 VTGLTVELAPGVLAATNRRGSDGGGWYASSGELTDEWREELMSESLDVHGKSFWSSGWSAKLHDEAAAEGAGDSSGGGPGEVLAREWTRAADVLRGLGGTEGGPTIGDVCSWDGLVVVHTDDDLLTMKGNSALLVQLGNLFYPCADELQVACFMALLSFLASRPEVLRVSPARAIKLLNAAAYHNVQSATLTESPMTDAGLDGTGEVIQIVDTGLDVNSCFFVDEDGGEGVAHGHYFEEIGVEASALSSWFTSWDFNLQIARVFTGGDFNFDLGRRKIVQYINLIRQ-DSETNSTANNSTNY-----GE----------SWATSAGEQYP--WIS---------------------------------AAEFAEDYAGGHGTHTAGSAAGAVVSSPSYSQTGDFTEVCEEGRELSCVGGCIDVDDTFGGDDLITTWDYQYFWAGYEAIDLDRLCPRLDCD---DLPEEYCLGDDEAETLQQHGGVARGAQLAIFDILDDYTGIGIEMAGNGMW 2054
            V GL+V L+PGVL A        GG  + +     +W  +L SES+++   SFWS        D  A     D           REW+RAA V+ GL  ++   T+G++C +  L + H  DDL+ ++G + LL   G  F     E + AC+M LLS LA++PEVLRVSP ++ +L NA A   VQSAT TE+P+ DAGLDGTGEVIQ+VD+GLD  SCFF D+DG + V HG  F+ + VE                ++ V     F FD+ RRKIVQYI L +  D  + S+  +S  Y     G+          S+   +GE Y   W                                      F +D  GGHGT TAGSAAGA+ +     +     E C       C GGCI   D    D ++               DLD  CP   CD   D+P   CL DD  E L QHGGVA GAQ+++FD+    T I    AGN +W
Sbjct:  173 VHGLSVRLSPGVLTA--------GGRESFAEAFLRDWHADLTSESVNMRRASFWS--------DSDADRSEYDV-------TRVREWSRAAAVVDGLA-SKSARTVGEICGFGKLKLRHVADDLVLVEGMNHLLP--GKRF---PSETRTACYMGLLSQLAAKPEVLRVSPLQSARLHNAVANALVQSATTTETPLLDAGLDGTGEVIQVVDSGLDETSCFFADDDGLQ-VEHGFLFDGVRVEDDG------------NVSTVLGNYSFPFDMSRRKIVQYIQLFKTPDQGSTSSGQDSFTYVSDYLGDPYDYDDSEYISYDDYSGEYYSDTWSEDSFCAGVLDDLSYHFSYLFDDTSYSSSSTSPSFTGGFHKDTVGGHGTWTAGSAAGAISAQSILEE-----EACYGDELPGCAGGCISASDV---DAMLDD----------STFDLDVFCPMYTCDGNTDIPSSECLSDDPVENLHQHGGVAPGAQISVFDVSYVGTEIYASFAGNDLW 625          

BLAST of mRNA_Ecto-sp8_S_contig9740.31478.1 vs. uniprot
Match: D8LQI1_ECTSI (Subtilisin n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LQI1_ECTSI)

HSP 1 Score: 234 bits (598), Expect = 2.330e-62
Identity = 168/461 (36.44%), Postives = 243/461 (52.71%), Query Frame = 3

Query:  684 GLTVELAPGVLAATNRRGSDGGGWYASSGELTDEWREELMSESLDVHGKSFWSSGWSAKLHDEAAAEGAGDSSGGGPGEVLAREWTRAADVLRGLGGTEGGPTIGDVCSWDGLVVVHTDDDLLTMKGNSALLV---QLGNLFYPCADELQVACFMALLSFLASRPEVLRVSPARAIKLLNAAAYHNVQSATLTESPMTDAGLDGTGEVIQIVDTGLDVNSCFFVDEDGGEGVAHGHYFEEIGVEASALSSWFTSWDFNLQIARVFTGGDFNFDLGRRKIVQYINLIRQDSETNSTANNSTNYGESWATSAGEQYPWISAAEFAEDYAGGHGTHTAGSAAGAVVSSPSYSQTGDFTEVCEEGRE-LSCVGGCIDVDDTFGGDDLITTWDYQYFWAGYEAIDLDRLCPRLDCDDLPEEYCLGDDEAETLQQHGGVARGAQLAIFDILDDYTGIGIEMAGNGMW 2054
            GL VEL+PG L + +       G  A + E   E  + L S SLD+H  + WS         +   +G GD      G +  REW+RAA ++     +E   T   VC W+ +VV H  +D+L + G   LL    ++       A EL++AC+M L+SFLASR EVL+V+       LNAAA   +QSA++  +P+TDAGLDG+GEVIQI+D G++  +CF    DG E V HG+YF+ +   AS+   + +S +        FTGGDF++D  RRK++QYI+LI  D+   S++                  P++SA  F  D   GHGTH A + AG+ +SSP+   +      C +G E + C GGCI    T   +            A Y   D++RLCP   C     + CL DD A+TL +HGG+A+GA++A  D+       G ++AGNG+W
Sbjct:  180 GLMVELSPGTLPSHS-------GGDAKAHEFIQELLKSLSSPSLDLHAITCWS---------DLVMKG-GDHLFSAAGALRGREWSRAATLVNERSESEQ-ETPSQVCGWEQVVVHHAANDVLLVAGLDHLLYDGREVSGASEEEAAELRMACYMGLVSFLASRHEVLKVAGWNRKVNLNAAARATIQSASVDSTPLTDAGLDGSGEVIQIIDAGVNETTCFLEHGDGTE-VEHGYYFDFV---ASSNPLFTSSSESRSMFKAEFTGGDFSYDSDRRKVIQYIDLINPDTGDGSSSG----------------MPFVSADSFVSDDDLGHGTHAATTVAGSTISSPAELVS------CADGSEVIGCAGGCITESSTGSSE------------ADY-GEDINRLCPAFGCSG---DQCLPDDTADTLSEHGGMAQGAKIAAIDVFYGDYSYG-DLAGNGLW 579