mRNA_Ecto-sp8_S_contig95820.31223.1 (mRNA) Ectocarpus species8 EcLAC_412

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_Ecto-sp8_S_contig95820.31223.1
Unique NamemRNA_Ecto-sp8_S_contig95820.31223.1
TypemRNA
OrganismEctocarpus species8 EcLAC_412 (Ectocarpus species8 EcLAC_412)
Homology
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BLAST of mRNA_Ecto-sp8_S_contig95820.31223.1 vs. uniprot
Match: A0A0F9YXK4_9ZZZZ (2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase n=1 Tax=marine sediment metagenome TaxID=412755 RepID=A0A0F9YXK4_9ZZZZ)

HSP 1 Score: 303 bits (777), Expect = 8.730e-104
Identity = 149/163 (91.41%), Postives = 157/163 (96.32%), Query Frame = 1 
Query:    1 SLEMLTHHYGDVYEHSPWVAEAAWQHGLTESHDTPDGLAELMGLMLQQASSDQQINVIRAHPDLAGKAAMAGELTQDSSSEQAGAGLDQCSPDEFARFEYLNETYKEKFGFPFVIAVKGLDRHAILAAFEERLHNDVATERETAIQQIIRIAHFRLVSRSTNS 489
            SLEMLTHHYGDVYEHSPWVAEAAWQHG+TE+HDTPDGLAELMGLMLQQASS+QQI+VIRAHPDLAGKAAMAGELTQDS+SEQAGAGLDQCSPDEFARFEYLNETYKEKFGFPFVIAVKGLDRHAILAAFE RLHND+ATER TAI+QIIRIA FRL+SR   S
Sbjct:   17 SLEMLTHHYGDVYEHSPWVAEAAWQHGITEAHDTPDGLAELMGLMLQQASSEQQISVIRAHPDLAGKAAMAGELTQDSTSEQAGAGLDQCSPDEFARFEYLNETYKEKFGFPFVIAVKGLDRHAILAAFEARLHNDIATERRTAIEQIIRIARFRLISRLAGS 179
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BLAST of mRNA_Ecto-sp8_S_contig95820.31223.1 vs. uniprot
Match: A0A265E1N3_9GAMM (2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase n=21 Tax=Halomonas TaxID=2745 RepID=A0A265E1N3_9GAMM)

HSP 1 Score: 292 bits (747), Expect = 3.620e-99
Identity = 144/163 (88.34%), Postives = 154/163 (94.48%), Query Frame = 1 
Query:    1 SLEMLTHHYGDVYEHSPWVAEAAWQHGLTESHDTPDGLAELMGLMLQQASSDQQINVIRAHPDLAGKAAMAGELTQDSSSEQAGAGLDQCSPDEFARFEYLNETYKEKFGFPFVIAVKGLDRHAILAAFEERLHNDVATERETAIQQIIRIAHFRLVSRSTNS 489
            SLEMLTHHYGDVYEHSPWVAEAAWQ GLTE+HDTPD LAELMGLMLQQASSDQQI VI+AHPDLAGKAAMAGELTQDS++EQAGAGLDQCSP EFARFEYLN  YKEKFGFPFVIAVKGLDRHAILAAFEERL+NDVA ER+TAI+QIIRIA FRL+ R+TN+
Sbjct:   17 SLEMLTHHYGDVYEHSPWVAEAAWQQGLTEAHDTPDALAELMGLMLQQASSDQQIKVIQAHPDLAGKAAMAGELTQDSTTEQAGAGLDQCSPGEFARFEYLNSAYKEKFGFPFVIAVKGLDRHAILAAFEERLNNDVAIERQTAIEQIIRIARFRLIDRATNT 179
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BLAST of mRNA_Ecto-sp8_S_contig95820.31223.1 vs. uniprot
Match: A0A365TQC0_9GAMM (2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase n=10 Tax=Halomonas TaxID=2745 RepID=A0A365TQC0_9GAMM)

HSP 1 Score: 285 bits (728), Expect = 2.480e-96
Identity = 140/160 (87.50%), Postives = 151/160 (94.38%), Query Frame = 1 
Query:    1 SLEMLTHHYGDVYEHSPWVAEAAWQHGLTESHDTPDGLAELMGLMLQQASSDQQINVIRAHPDLAGKAAMAGELTQDSSSEQAGAGLDQCSPDEFARFEYLNETYKEKFGFPFVIAVKGLDRHAILAAFEERLHNDVATERETAIQQIIRIAHFRLVSRS 480
            SLEMLTHHYGD+YEHSPWVAEAAWQHGLTE+HDTPD LAELMGLMLQQASS+QQI+VIRAHPDLAGKAAMAGELTQDS+SEQAGAGLDQCSP+EFARFEYLN  YKEKFGFPFVIAVKGLDRH IL AFE RL+ND ATER+TAI+QIIRIA FRL +R+
Sbjct:   17 SLEMLTHHYGDIYEHSPWVAEAAWQHGLTEAHDTPDALAELMGLMLQQASSEQQISVIRAHPDLAGKAAMAGELTQDSTSEQAGAGLDQCSPEEFARFEYLNVAYKEKFGFPFVIAVKGLDRHGILDAFEARLNNDAATERQTAIEQIIRIARFRLRARA 176
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BLAST of mRNA_Ecto-sp8_S_contig95820.31223.1 vs. uniprot
Match: A0A0D5M0B7_9GAMM (2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase n=1 Tax=Halomonas sp. KO116 TaxID=1504981 RepID=A0A0D5M0B7_9GAMM)

HSP 1 Score: 275 bits (704), Expect = 1.130e-92
Identity = 137/160 (85.62%), Postives = 146/160 (91.25%), Query Frame = 1 
Query:    1 SLEMLTHHYGDVYEHSPWVAEAAWQHGLTESHDTPDGLAELMGLMLQQASSDQQINVIRAHPDLAGKAAMAGELTQDSSSEQAGAGLDQCSPDEFARFEYLNETYKEKFGFPFVIAVKGLDRHAILAAFEERLHNDVATERETAIQQIIRIAHFRLVSRS 480
            SLEMLTHHYGD+YEHSPWVAEAAW HGLTE+HDTPD LAELMGLMLQQASS+QQI+VIRAHPDLAGKAAMAGELTQDS+SEQAGAGLDQCSP EFARFE LN  YKEKFGFPFVIAVKGLDRH IL AFE RLHND A ER+TAI+QII IA FRL +R+
Sbjct:   17 SLEMLTHHYGDIYEHSPWVAEAAWHHGLTEAHDTPDALAELMGLMLQQASSEQQISVIRAHPDLAGKAAMAGELTQDSTSEQAGAGLDQCSPAEFARFEDLNAAYKEKFGFPFVIAVKGLDRHGILDAFEARLHNDAAAERQTAIEQIICIARFRLRARA 176
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BLAST of mRNA_Ecto-sp8_S_contig95820.31223.1 vs. uniprot
Match: A0A651DF48_HALSX (2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase n=1 Tax=Halomonas sp. TaxID=1486246 RepID=A0A651DF48_HALSX)

HSP 1 Score: 273 bits (698), Expect = 5.170e-92
Identity = 133/158 (84.18%), Postives = 147/158 (93.04%), Query Frame = 1 
Query:   10 MLTHHYGDVYEHSPWVAEAAWQHGLTESHDTPDGLAELMGLMLQQASSDQQINVIRAHPDLAGKAAMAGELTQDSSSEQAGAGLDQCSPDEFARFEYLNETYKEKFGFPFVIAVKGLDRHAILAAFEERLHNDVATERETAIQQIIRIAHFRLVSRST 483
            M THHYGDVYEHSPWVAEAAW+ GLTE+HD P+ LAELMGLMLQQASS+QQI VI+AHPDLAGKAA+AGELTQDS+SEQAGAGLDQCSP+E ARFEYLNETYK++FGFPFVIAVKGLDRHAILAAFEERL ND  TER+TAI+QIIRIA FRL+ R+T
Sbjct:    1 MFTHHYGDVYEHSPWVAEAAWRKGLTEAHDAPNALAELMGLMLQQASSEQQIQVIQAHPDLAGKAALAGELTQDSTSEQAGAGLDQCSPEELARFEYLNETYKQQFGFPFVIAVKGLDRHAILAAFEERLGNDAVTERQTAIEQIIRIARFRLIDRAT 158
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BLAST of mRNA_Ecto-sp8_S_contig95820.31223.1 vs. uniprot
Match: A0A511UT06_9GAMM (2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase n=2 Tax=Halovibrio variabilis TaxID=31910 RepID=A0A511UT06_9GAMM)

HSP 1 Score: 271 bits (692), Expect = 4.400e-91
Identity = 135/159 (84.91%), Postives = 146/159 (91.82%), Query Frame = 1 
Query:   10 MLTHHYGDVYEHSPWVAEAAWQHGLTESHDTPDGLAELMGLMLQQASSDQQINVIRAHPDLAGKAAMAGELTQDSSSEQAGAGLDQCSPDEFARFEYLNETYKEKFGFPFVIAVKGLDRHAILAAFEERLHNDVATERETAIQQIIRIAHFRLVSRSTN 486
            ML  HYGDVYEHSPWVAEAAW+HGLTE+HDTPD LAELMGLMLQQASS+QQI  I+AHPDLAGKAAMAGELTQDS+SEQAGAGLDQCSP+EFARFE LN  YKEKFGFPFVIAVKGLDRHAILAAFEERL+ND  TER+TAI+QIIRIA FRL  R+T+
Sbjct:    1 MLALHYGDVYEHSPWVAEAAWRHGLTEAHDTPDALAELMGLMLQQASSEQQIKAIQAHPDLAGKAAMAGELTQDSTSEQAGAGLDQCSPEEFARFETLNAVYKEKFGFPFVIAVKGLDRHAILAAFEERLNNDKPTERQTAIEQIIRIARFRLRDRATS 159
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BLAST of mRNA_Ecto-sp8_S_contig95820.31223.1 vs. uniprot
Match: A0A4U3CA44_9GAMM (2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase n=3 Tax=Halomonas TaxID=2745 RepID=A0A4U3CA44_9GAMM)

HSP 1 Score: 263 bits (671), Expect = 1.580e-87
Identity = 130/160 (81.25%), Postives = 144/160 (90.00%), Query Frame = 1 
Query:    1 SLEMLTHHYGDVYEHSPWVAEAAWQHGLTESHDTPDGLAELMGLMLQQASSDQQINVIRAHPDLAGKAAMAGELTQDSSSEQAGAGLDQCSPDEFARFEYLNETYKEKFGFPFVIAVKGLDRHAILAAFEERLHNDVATERETAIQQIIRIAHFRLVSRS 480
            SLE    HYGDVYEHSPWVAEAAWQHGL+E HDTPD LAELMGLMLQQA+ +QQI VI+AHPDLAGKAA+AGELT+DS+SEQAGAGLDQCSP+EFARFE LN  YKEKFGFPFV+AVKGLDR+ ILAAFEERL ND+A ERETA+QQIIRIA FRL +R+
Sbjct:   24 SLETFVKHYGDVYEHSPWVAEAAWQHGLSEPHDTPDALAELMGLMLQQATPEQQIAVIQAHPDLAGKAALAGELTKDSTSEQAGAGLDQCSPEEFARFERLNAAYKEKFGFPFVVAVKGLDRYDILAAFEERLENDLAQERETAVQQIIRIARFRLRARA 183
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BLAST of mRNA_Ecto-sp8_S_contig95820.31223.1 vs. uniprot
Match: A0A2A3XDZ4_9GAMM (2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase n=3 Tax=Halomonas TaxID=2745 RepID=A0A2A3XDZ4_9GAMM)

HSP 1 Score: 261 bits (668), Expect = 3.560e-87
Identity = 131/163 (80.37%), Postives = 145/163 (88.96%), Query Frame = 1 
Query:    1 SLEMLTHHYGDVYEHSPWVAEAAWQHGLTESHDTPDGLAELMGLMLQQASSDQQINVIRAHPDLAGKAAMAGELTQDSSSEQAGAGLDQCSPDEFARFEYLNETYKEKFGFPFVIAVKGLDRHAILAAFEERLHNDVATERETAIQQIIRIAHFRLVSRSTNS 489
            SLEMLT HYGDVYEHSPWVAEAAW HGL E HD PDGLA+LMGLMLQQASS+QQINVIRAHPDLAGKAA++GELTQDS+SEQAGAGL  CS DEFA+FE LNE YK+KFGFPFVIAVKGLDR AILAAFEERL+N  ATER+TAI+ IIRIA  RL+ R++ +
Sbjct:   17 SLEMLTLHYGDVYEHSPWVAEAAWHHGLNEEHDAPDGLAQLMGLMLQQASSEQQINVIRAHPDLAGKAAISGELTQDSTSEQAGAGLHHCSADEFAQFESLNEAYKKKFGFPFVIAVKGLDRGAILAAFEERLNNAAATERQTAIEHIIRIACLRLIDRASKA 179
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BLAST of mRNA_Ecto-sp8_S_contig95820.31223.1 vs. uniprot
Match: UPI0009F2E349 (2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase n=1 Tax=Halomonas lionensis TaxID=1144478 RepID=UPI0009F2E349)

HSP 1 Score: 261 bits (666), Expect = 7.170e-87
Identity = 130/160 (81.25%), Postives = 143/160 (89.38%), Query Frame = 1 
Query:    1 SLEMLTHHYGDVYEHSPWVAEAAWQHGLTESHDTPDGLAELMGLMLQQASSDQQINVIRAHPDLAGKAAMAGELTQDSSSEQAGAGLDQCSPDEFARFEYLNETYKEKFGFPFVIAVKGLDRHAILAAFEERLHNDVATERETAIQQIIRIAHFRLVSRS 480
            SLE    HYGDVYEHSPWVAEA WQHGL+ESHD PD LAELMGLMLQQA+S+QQI+VI+AHPDLAGKAA+AGELTQDS++EQAGAGLDQCSP EFARFE LN  YKEKFGFPFVIAVKGLDR+ ILAAFEERL N +A ERETAI+QIIRIA FRL +R+
Sbjct:   17 SLEAFVQHYGDVYEHSPWVAEATWQHGLSESHDAPDALAELMGLMLQQATSEQQIDVIQAHPDLAGKAALAGELTQDSTNEQAGAGLDQCSPKEFARFEQLNAAYKEKFGFPFVIAVKGLDRYDILAAFEERLENGLAQERETAIKQIIRIARFRLRARA 176
[Close]
BLAST of mRNA_Ecto-sp8_S_contig95820.31223.1 vs. uniprot
Match: A0A7C9JR45_9GAMM (2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase n=2 Tax=Halomonadaceae TaxID=28256 RepID=A0A7C9JR45_9GAMM)

HSP 1 Score: 259 bits (663), Expect = 3.650e-86
Identity = 130/159 (81.76%), Postives = 142/159 (89.31%), Query Frame = 1 
Query:    1 SLEMLTHHYGDVYEHSPWVAEAAWQHGLTESHDTPDGLAELMGLMLQQASSDQQINVIRAHPDLAGKAAMAGELTQDSSSEQAGAGLDQCSPDEFARFEYLNETYKEKFGFPFVIAVKGLDRHAILAAFEERLHNDVATERETAIQQIIRIAHFRLVSR 477
            SLE    HYGDVYEHSPWVAEAAW+ GLT++H++PD LAELMGLMLQQA  D QI VI+AHPDLAGKAAMAGELTQDS+ EQAGAGLDQC+PDEFARFEYLN  YKEKFGFPFVIAVKGLDRHAILAAFEERL+N +A ERETAI+QIIRIA FRL +R
Sbjct:   34 SLETFVLHYGDVYEHSPWVAEAAWKKGLTDTHNSPDALAELMGLMLQQAEPDLQIAVIQAHPDLAGKAAMAGELTQDSTKEQAGAGLDQCTPDEFARFEYLNAAYKEKFGFPFVIAVKGLDRHAILAAFEERLNNGLAEERETAIEQIIRIARFRLRAR 192
The following BLAST results are available for this feature:
BLAST of mRNA_Ecto-sp8_S_contig95820.31223.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A0F9YXK4_9ZZZZ8.730e-10491.412-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline deca... [more]
A0A265E1N3_9GAMM3.620e-9988.342-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline deca... [more]
A0A365TQC0_9GAMM2.480e-9687.502-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline deca... [more]
A0A0D5M0B7_9GAMM1.130e-9285.632-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline deca... [more]
A0A651DF48_HALSX5.170e-9284.182-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline deca... [more]
A0A511UT06_9GAMM4.400e-9184.912-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline deca... [more]
A0A4U3CA44_9GAMM1.580e-8781.252-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline deca... [more]
A0A2A3XDZ4_9GAMM3.560e-8780.372-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline deca... [more]
UPI0009F2E3497.170e-8781.252-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline deca... [more]
A0A7C9JR45_9GAMM3.650e-8681.762-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline deca... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
Ecto-sp8_S_contig95820contigEcto-sp8_S_contig95820:2..493 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Ectocarpus species8 EcLAC_412 OGS1.02022-07-08
Properties
Property NameValue
Taxonomic scopeBacteria
Stop1
Start1
Seed ortholog score281.2
Seed ortholog evalue3.1e-73
Seed eggNOG ortholog1380358.JADJ01000017_gene1467
Preferred nameuraD
Model size492
KEGG rclassRC00166,RC00300,RC01551,RC02107,RC02551
KEGG koko:K00365,ko:K01452,ko:K13485,ko:K16838
KEGG ReactionR02106,R02333,R06604,R07981
KEGG Pathwayko00230,ko00232,ko00520,ko01100,ko01120,map00230,map00232,map00520,map01100,map01120
KEGG ModuleM00546
Hectar predicted targeting categoryother localisation
Exons1
EggNOG free text desc.protein conserved in bacteria
EggNOG OGs1RH9S@1224,1SAAD@1236,1XS3S@135619,COG3195@1,COG3195@2
EC1.7.3.3,3.5.1.41,4.1.1.97
Cds size483
COG Functional cat.S
Best tax levelOceanospirillales
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko00002,ko01000
Relationships

The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1681145320.3139596-UTR-Ecto-sp8_S_contig95820:1..101681145320.3139596-UTR-Ecto-sp8_S_contig95820:1..10Ectocarpus species8 EcLAC_412UTREcto-sp8_S_contig95820 2..10 +


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1681145320.330513-CDS-Ecto-sp8_S_contig95820:10..4931681145320.330513-CDS-Ecto-sp8_S_contig95820:10..493Ectocarpus species8 EcLAC_412CDSEcto-sp8_S_contig95820 11..493 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_Ecto-sp8_S_contig95820.31223.1prot_Ecto-sp8_S_contig95820.31223.1Ectocarpus species8 EcLAC_412polypeptideEcto-sp8_S_contig95820 11..493 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_Ecto-sp8_S_contig95820.31223.1

>prot_Ecto-sp8_S_contig95820.31223.1 ID=prot_Ecto-sp8_S_contig95820.31223.1|Name=mRNA_Ecto-sp8_S_contig95820.31223.1|organism=Ectocarpus species8 EcLAC_412|type=polypeptide|length=161bp
MLTHHYGDVYEHSPWVAEAAWQHGLTESHDTPDGLAELMGLMLQQASSDQ
QINVIRAHPDLAGKAAMAGELTQDSSSEQAGAGLDQCSPDEFARFEYLNE
TYKEKFGFPFVIAVKGLDRHAILAAFEERLHNDVATERETAIQQIIRIAH
FRLVSRSTNS*
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mRNA from alignment at Ecto-sp8_S_contig95820:2..493+

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_Ecto-sp8_S_contig95820.31223.1 ID=mRNA_Ecto-sp8_S_contig95820.31223.1|Name=mRNA_Ecto-sp8_S_contig95820.31223.1|organism=Ectocarpus species8 EcLAC_412|type=mRNA|length=492bp|location=Sequence derived from alignment at Ecto-sp8_S_contig95820:2..493+ (Ectocarpus species8 EcLAC_412)
AGCCTTGAGATGCTTACTCATCACTACGGTGATGTTTATGAGCACTCACC
ATGGGTCGCTGAAGCGGCTTGGCAGCACGGCCTCACTGAATCGCACGACA
CCCCAGATGGGTTGGCTGAACTCATGGGGCTGATGCTGCAGCAGGCAAGT
AGCGACCAGCAAATCAACGTGATCCGTGCCCACCCAGACCTGGCTGGCAA
AGCCGCGATGGCTGGCGAATTGACTCAAGACTCAAGCAGCGAACAAGCCG
GGGCAGGGTTAGATCAATGCAGCCCTGATGAATTTGCGCGTTTTGAATAC
CTCAATGAGACGTATAAGGAAAAATTTGGTTTTCCTTTTGTCATCGCAGT
GAAAGGGTTAGATCGCCACGCTATTTTGGCCGCCTTTGAAGAGCGCTTGC
ACAATGATGTGGCTACTGAGCGTGAAACAGCCATTCAGCAAATCATCCGT
ATTGCACACTTTCGCTTAGTCAGTCGCTCAACAAATAGCTAA
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Coding sequence (CDS) from alignment at Ecto-sp8_S_contig95820:2..493+

>mRNA_Ecto-sp8_S_contig95820.31223.1 ID=mRNA_Ecto-sp8_S_contig95820.31223.1|Name=mRNA_Ecto-sp8_S_contig95820.31223.1|organism=Ectocarpus species8 EcLAC_412|type=CDS|length=483bp|location=Sequence derived from alignment at Ecto-sp8_S_contig95820:2..493+ (Ectocarpus species8 EcLAC_412)
ATGCTTACTCATCACTACGGTGATGTTTATGAGCACTCACCATGGGTCGC
TGAAGCGGCTTGGCAGCACGGCCTCACTGAATCGCACGACACCCCAGATG
GGTTGGCTGAACTCATGGGGCTGATGCTGCAGCAGGCAAGTAGCGACCAG
CAAATCAACGTGATCCGTGCCCACCCAGACCTGGCTGGCAAAGCCGCGAT
GGCTGGCGAATTGACTCAAGACTCAAGCAGCGAACAAGCCGGGGCAGGGT
TAGATCAATGCAGCCCTGATGAATTTGCGCGTTTTGAATACCTCAATGAG
ACGTATAAGGAAAAATTTGGTTTTCCTTTTGTCATCGCAGTGAAAGGGTT
AGATCGCCACGCTATTTTGGCCGCCTTTGAAGAGCGCTTGCACAATGATG
TGGCTACTGAGCGTGAAACAGCCATTCAGCAAATCATCCGTATTGCACAC
TTTCGCTTAGTCAGTCGCTCAACAAATAGCTAA
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