mRNA_Ecto-sp8_S_contig10978.1343.1 (mRNA) Ectocarpus species8 EcLAC_412

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_Ecto-sp8_S_contig10978.1343.1
Unique NamemRNA_Ecto-sp8_S_contig10978.1343.1
TypemRNA
OrganismEctocarpus species8 EcLAC_412 (Ectocarpus species8 EcLAC_412)
Homology
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BLAST of mRNA_Ecto-sp8_S_contig10978.1343.1 vs. uniprot
Match: D8LBE9_ECTSI (Ferredoxin n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LBE9_ECTSI)

HSP 1 Score: 285 bits (729), Expect = 7.730e-97
Identity = 157/159 (98.74%), Postives = 159/159 (100.00%), Query Frame = 1 
Query:    1 MKSFLFVALILLAAVLQISNGFVVAPSSSLNVRRQSHSGMQMLFGXXXXXXXXXXXIVVKVQQKTGFKETELTLAGKTNLRKALMDNKIDIYPLQGKIYNCGGGGSCGTCAVNVKAGAQNCSPKGPGEKKLLEKNKRPASYRLSCCTMVSGPVTVQTKP 477
            MKSFLFVALI+LAAVLQISNGFVVAPSSSLNVRRQSHSGMQMLFGXXXXXXXXXXXIVVKVQQKTGFKETELTLAGKTNLRKALMDNKIDIYPLQGKIYNCGGGGSCGTCAVNVKAGAQNCSPKGPGEKKLL+KNKRPASYRLSCCTMVSGPVTVQTKP
Sbjct:    1 MKSFLFVALIVLAAVLQISNGFVVAPSSSLNVRRQSHSGMQMLFGXXXXXXXXXXXIVVKVQQKTGFKETELTLAGKTNLRKALMDNKIDIYPLQGKIYNCGGGGSCGTCAVNVKAGAQNCSPKGPGEKKLLDKNKRPASYRLSCCTMVSGPVTVQTKP 159
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BLAST of mRNA_Ecto-sp8_S_contig10978.1343.1 vs. uniprot
Match: A0A836CNT2_9STRA (Ferredoxin n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A836CNT2_9STRA)

HSP 1 Score: 140 bits (353), Expect = 1.090e-39
Identity = 80/146 (54.79%), Postives = 97/146 (66.44%), Query Frame = 1 
Query:   40 AVLQISNGFVVAPSSSLNVRRQSHSGMQMLFGXXXXXXXXXXXIVVKVQQKTGFKETELTLAGKTNLRKALMDNKIDIYPLQGKIYNCGGGGSCGTCAVNVKAGAQNCSPKGPGEKKLLEKNKRPASYRLSCCTMVSGPVTVQTKP 477
            +V+  +  F+VAP+    V+R + +  +MLFG           I V VQQK  FK+T + +    NLRKALM   I+IYPLQGKIYNCGGGGSCGTC VNV AG  NCSPKGP EKKLL    +P +YRLSCCT V+G +TV TKP
Sbjct:   15 SVMLAAEAFLVAPALQRPVQRDTLTP-RMLFGKKAAPAKSKG-IEVTVQQKY-FKDTVVAMEQPGNLRKALMGAGIEIYPLQGKIYNCGGGGSCGTCTVNVVAGGNNCSPKGPAEKKLL--GNKPETYRLSCCTKVNGNITVATKP 155
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BLAST of mRNA_Ecto-sp8_S_contig10978.1343.1 vs. uniprot
Match: A0A7S2UXM9_9STRA (Hypothetical protein n=1 Tax=Fibrocapsa japonica TaxID=94617 RepID=A0A7S2UXM9_9STRA)

HSP 1 Score: 134 bits (338), Expect = 1.920e-37
Identity = 74/154 (48.05%), Postives = 94/154 (61.04%), Query Frame = 1 
Query:   16 FVALILLAAVLQISNGFVVAPSSSLNVRRQSHSGMQMLFGXXXXXXXXXXXIVVKVQQKTGFKETELTLAGKTNLRKALMDNKIDIYPLQGKIYNCGGGGSCGTCAVNVKAGAQNCSPKGPGEKKLLEKNKRPASYRLSCCTMVSGPVTVQTKP 477
            F  +I L   +  ++ F+    +   V+  S + +QMLFG             + V+Q+ GF + +L   G  NLRK LM  KIDIYPLQGK+ NCGGGG CGTCAV V  GA N SPK P EKK+L  +K+P +YRLSCCT VSG  TV+TKP
Sbjct:    3 FTVVIFLVCAISGASAFLGQSLALNRVQASSSTSLQMLFGSKKKAAPVKGKYSITVKQR-GFTDFDLETEGTANLRKTLMAQKIDIYPLQGKLTNCGGGGVCGTCAVEVMDGANNLSPKAPNEKKVL--SKKPENYRLSCCTRVSGACTVRTKP 153
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BLAST of mRNA_Ecto-sp8_S_contig10978.1343.1 vs. uniprot
Match: W7TM71_9STRA (Ferredoxin n=2 Tax=Monodopsidaceae TaxID=425072 RepID=W7TM71_9STRA)

HSP 1 Score: 113 bits (283), Expect = 4.540e-29
Identity = 61/103 (59.22%), Postives = 70/103 (67.96%), Query Frame = 1 
Query:  175 VKVQQKTGFKETELTL--AGKTNLRKALMDNKIDIYPLQGKIYNCGGGGSCGTCAVNVKAGAQNCSPKGPGEKKLLEKNKRPASYRLSCCTMVSGPVTVQTKP 477
            V VQQK  +K  EL +      NLRK LM NKIDIYPLQGKIYNCGGGG CGTCA+ +  GA+N +PK P E K L   K+    RL+CCT  SG VTV+TKP
Sbjct:   58 VTVQQKF-YKNAELEVEAGAPVNLRKELMKNKIDIYPLQGKIYNCGGGGQCGTCAIQIIKGAKNLNPKSPVEVKALG-GKKGDDIRLACCTRASGDVTVKTKP 158
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BLAST of mRNA_Ecto-sp8_S_contig10978.1343.1 vs. uniprot
Match: M1VFK1_CYAM1 (2Fe-2S ferredoxin-type domain-containing protein n=1 Tax=Cyanidioschyzon merolae (strain NIES-3377 / 10D) TaxID=280699 RepID=M1VFK1_CYAM1)

HSP 1 Score: 108 bits (270), Expect = 9.920e-27
Identity = 45/82 (54.88%), Postives = 62/82 (75.61%), Query Frame = 1 
Query:  232 TNLRKALMDNKIDIYPLQGKIYNCGGGGSCGTCAVNVKAGAQNCSPKGPGEKKLLEKNKRPASYRLSCCTMVSGPVTVQTKP 477
            +NLRK L++NKID+Y L+GK+ NCGGGG CGTC V++  G  N +P+G  E ++ E  + P S+RLSCCT + GP+TV+TKP
Sbjct:  109 SNLRKVLLENKIDVYTLRGKLTNCGGGGQCGTCIVDITEGQYNTNPRGWREARIFENKQAPDSWRLSCCTKIEGPITVRTKP 190
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BLAST of mRNA_Ecto-sp8_S_contig10978.1343.1 vs. uniprot
Match: UPI00055BF685 ((2Fe-2S)-binding protein n=1 Tax=Myxosarcina sp. GI1 TaxID=1541065 RepID=UPI00055BF685)

HSP 1 Score: 96.7 bits (239), Expect = 4.300e-23
Identity = 46/90 (51.11%), Postives = 62/90 (68.89%), Query Frame = 1 
Query:  208 TELTLAGKTNLRKALMDNKIDIYPLQGKIYNCGGGGSCGTCAVNVKAGAQNCSPKGPGEKKLLEKNKRPASYRLSCCTMVSGPVTVQTKP 477
            TE+  A  +NLR+  + N +DIY L+GK+ NCGG G C TC V++  G +N SP+   E+K L   K+P SYRLSC T+V GP+TV+TKP
Sbjct:   17 TEVVAANGSNLREKALQNNVDIYKLKGKLTNCGGVGQCSTCIVDIVEGMENLSPRTDFEQKRL--GKKPDSYRLSCQTVVKGPITVRTKP 104
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BLAST of mRNA_Ecto-sp8_S_contig10978.1343.1 vs. uniprot
Match: A0A835YVE2_9STRA (2Fe-2S ferredoxin-type domain-containing protein n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835YVE2_9STRA)

HSP 1 Score: 95.5 bits (236), Expect = 1.800e-22
Identity = 45/81 (55.56%), Postives = 57/81 (70.37%), Query Frame = 1 
Query:  235 NLRKALMDNKIDIYPLQGKIYNCGGGGSCGTCAVNVKAGAQNCSPKGPGEKKLLEKNKRPASYRLSCCTMVSGPVTVQTKP 477
            NLRK L+DNK+D+Y    K+  CGGGG+CGTC V V  G +NCS KG GE+K+L+     A  RL+CCT V+GPV+V  KP
Sbjct:   40 NLRKLLLDNKVDLYKPLDKLKQCGGGGTCGTCRVRVTEGMENCSKKGLGEEKMLKAVG--ADIRLACCTRVTGPVSVTVKP 118
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BLAST of mRNA_Ecto-sp8_S_contig10978.1343.1 vs. uniprot
Match: A0A218QTJ0_9CYAN (Ferredoxin n=3 Tax=Cyanobacteria TaxID=1117 RepID=A0A218QTJ0_9CYAN)

HSP 1 Score: 91.3 bits (225), Expect = 4.700e-21
Identity = 44/89 (49.44%), Postives = 59/89 (66.29%), Query Frame = 1 
Query:  211 ELTLAGKTNLRKALMDNKIDIYPLQGKIYNCGGGGSCGTCAVNVKAGAQNCSPKGPGEKKLLEKNKRPASYRLSCCTMVSGPVTVQTKP 477
            E+  A   NLR   M N IDIY   GK+ NCGGGG+CGTC V +  G +N SP+   E ++L+  K+P +YRL+C T+V+G V+V TKP
Sbjct:   12 EVIAANGANLRLKAMQNNIDIYKFIGKMTNCGGGGNCGTCIVEIVEGIENLSPRTDAENRILK--KKPENYRLACQTVVNGAVSVVTKP 98
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BLAST of mRNA_Ecto-sp8_S_contig10978.1343.1 vs. uniprot
Match: A0A6P0JWH0_9CYAN ((2Fe-2S)-binding protein n=1 Tax=Kamptonema sp. SIO4C4 TaxID=2607773 RepID=A0A6P0JWH0_9CYAN)

HSP 1 Score: 90.1 bits (222), Expect = 1.490e-20
Identity = 43/89 (48.31%), Postives = 61/89 (68.54%), Query Frame = 1 
Query:  211 ELTLAGKTNLRKALMDNKIDIYPLQGKIYNCGGGGSCGTCAVNVKAGAQNCSPKGPGEKKLLEKNKRPASYRLSCCTMVSGPVTVQTKP 477
            ++  A   N+R+  + N +D+Y L+GK+ NCGG G CGTC V V  G +N S +   E++ L+  K+PASYRLSC T+V+GPV V+TKP
Sbjct:   15 DVVAADGANMREKALQNGVDLYTLRGKLTNCGGYGQCGTCVVEVIEGMENLSERTQFEERKLK--KKPASYRLSCQTLVNGPVKVRTKP 101
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BLAST of mRNA_Ecto-sp8_S_contig10978.1343.1 vs. uniprot
Match: A0A1Z4LLR8_9CYAN (Ferredoxin n=1 Tax=Calothrix parasitica NIES-267 TaxID=1973488 RepID=A0A1Z4LLR8_9CYAN)

HSP 1 Score: 89.7 bits (221), Expect = 1.880e-20
Identity = 44/89 (49.44%), Postives = 57/89 (64.04%), Query Frame = 1 
Query:  211 ELTLAGKTNLRKALMDNKIDIYPLQGKIYNCGGGGSCGTCAVNVKAGAQNCSPKGPGEKKLLEKNKRPASYRLSCCTMVSGPVTVQTKP 477
            E+  A   NLR   M N IDIY + GK+ NCGG G CGTC V +  G +N SP+   E K+L+  K+P +YRL+C  +VSG V+V TKP
Sbjct:   12 EVVAADGANLRLKAMQNNIDIYKVWGKMMNCGGAGQCGTCIVEITEGIENLSPRTDVENKILK--KKPENYRLACQALVSGAVSVVTKP 98
The following BLAST results are available for this feature:
BLAST of mRNA_Ecto-sp8_S_contig10978.1343.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
D8LBE9_ECTSI7.730e-9798.74Ferredoxin n=1 Tax=Ectocarpus siliculosus TaxID=28... [more]
A0A836CNT2_9STRA1.090e-3954.79Ferredoxin n=1 Tax=Tribonema minus TaxID=303371 Re... [more]
A0A7S2UXM9_9STRA1.920e-3748.05Hypothetical protein n=1 Tax=Fibrocapsa japonica T... [more]
W7TM71_9STRA4.540e-2959.22Ferredoxin n=2 Tax=Monodopsidaceae TaxID=425072 Re... [more]
M1VFK1_CYAM19.920e-2754.882Fe-2S ferredoxin-type domain-containing protein n... [more]
UPI00055BF6854.300e-2351.11(2Fe-2S)-binding protein n=1 Tax=Myxosarcina sp. G... [more]
A0A835YVE2_9STRA1.800e-2255.562Fe-2S ferredoxin-type domain-containing protein n... [more]
A0A218QTJ0_9CYAN4.700e-2149.44Ferredoxin n=3 Tax=Cyanobacteria TaxID=1117 RepID=... [more]
A0A6P0JWH0_9CYAN1.490e-2048.31(2Fe-2S)-binding protein n=1 Tax=Kamptonema sp. SI... [more]
A0A1Z4LLR8_9CYAN1.880e-2049.44Ferredoxin n=1 Tax=Calothrix parasitica NIES-267 T... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
Ecto-sp8_S_contig10978contigEcto-sp8_S_contig10978:1187..3879 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Ectocarpus species8 EcLAC_412 OGS1.02022-07-08
Properties
Property NameValue
Taxonomic scopeEukaryota
Stop1
Start1
Seed ortholog score109.8
Seed ortholog evalue1.2e-21
Seed eggNOG ortholog45157.CME070CT
Model size480
Hectar predicted targeting categorysignal peptide
Exons2
EggNOG free text desc.2Fe-2S iron-sulfur cluster binding domain
EggNOG OGs2RXME@2759,COG0633@1
Cds size480
COG Functional cat.C
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1681143184.7950895-CDS-Ecto-sp8_S_contig10978:1186..13811681143184.7950895-CDS-Ecto-sp8_S_contig10978:1186..1381Ectocarpus species8 EcLAC_412CDSEcto-sp8_S_contig10978 1187..1381 -
1681143184.806793-CDS-Ecto-sp8_S_contig10978:3594..38791681143184.806793-CDS-Ecto-sp8_S_contig10978:3594..3879Ectocarpus species8 EcLAC_412CDSEcto-sp8_S_contig10978 3595..3879 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_Ecto-sp8_S_contig10978.1343.1prot_Ecto-sp8_S_contig10978.1343.1Ectocarpus species8 EcLAC_412polypeptideEcto-sp8_S_contig10978 1187..3879 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_Ecto-sp8_S_contig10978.1343.1

>prot_Ecto-sp8_S_contig10978.1343.1 ID=prot_Ecto-sp8_S_contig10978.1343.1|Name=mRNA_Ecto-sp8_S_contig10978.1343.1|organism=Ectocarpus species8 EcLAC_412|type=polypeptide|length=160bp
MKSFLFVALILLAAVLQISNGFVVAPSSSLNVRRQSHSGMQMLFGGGGAK
KKGAGGIVVKVQQKTGFKETELTLAGKTNLRKALMDNKIDIYPLQGKIYN
CGGGGSCGTCAVNVKAGAQNCSPKGPGEKKLLEKNKRPASYRLSCCTMVS
GPVTVQTKP*
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mRNA from alignment at Ecto-sp8_S_contig10978:1187..3879-

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_Ecto-sp8_S_contig10978.1343.1 ID=mRNA_Ecto-sp8_S_contig10978.1343.1|Name=mRNA_Ecto-sp8_S_contig10978.1343.1|organism=Ectocarpus species8 EcLAC_412|type=mRNA|length=2693bp|location=Sequence derived from alignment at Ecto-sp8_S_contig10978:1187..3879- (Ectocarpus species8 EcLAC_412)
ATGAAGTCCTTTTTGTTCGTGGCGCTCATCCTTCTTGCAGCTGTGCTGCA
GATATCAAATGGGTTTGTAGTGGCTCCATCGTCATCGCTCAATGTGCGAC
GGCAGTCACACTCTGGTATGCAGATGCTCTTCGGCGGCGGAGGTGCCAAG
AAAAAAGGTGCTGGAGGGATCGTGGTGAAGGTGCAACAGAAGACAGGGTT
CAAGGAAACGGAGCTCACGCTGGCGGGCAAGACGAACCTGAGGAAGGCTC
TGATGGACAACAAAATTGACATCTACCCGCTGCAGGTGAGCGAAAGTAAG
GTCCTTGAAATAGAGTACAAGTAGGTGCTTTTCACGTAAATCCTGCAAAC
ACCCATACGTACGTGTCAGTCAGTACGTGTTCGTTCCTGATCATGTTTTA
TTGTCCTACGACGTCATACCAGATACATACGTTTTAGACTAAAACAGCAT
AGACCTGAAATAGTTTGCACTCTGCTATTGCCTTGCGTCGATCTCTGGTG
CATGGAAAGGGACCCAACTTGTTGGTGTGATGTGCTACACTGCCCCCACG
CCGTAGCAAACATCTGGTACACAGGAAGACCGCGCAACCCACGGTAACAC
TCAAAACATGTACTTCTACCATACTTCGAGAGGTGCTTAGACTGGCGCCT
GAACGTATGGAAAGATCCCAGACACTCGCGCCCTCGAACATTGATTTCCA
CCATATTGCACGCTCAGCATGGTGTCGGTCTGAATCTTTGATTCTCTGCA
AGGCCGCCGAACCTGAGGTACGTTGTCACATTTACGCTGGAGAGTAAATG
TCCCGTGATTGTGGTACACCTTTGTTTTACATTTGTACTTTTAAAGTAAA
TGTCATGTCATGTCCTTCGTCCATTACGAAAGTTAGGTTGTCCACTTCAA
ATAAATACAATATAGTTTTATGTGTCTTGTCAACATGTTATTATTTCTGT
TGGCCCAAAACTCACGTAAGTATATGGTAGGTAGTTTTCGTGGAGTGCGA
GCTCTATCGCCACTAATAAACTGCAGGGGTGATGTGGGACTTGTGTCTCC
TGAACCAACAAACCTTTAACAAACCTTTCTGTGCCTGTAACGTCCAGCGT
CATCGGAAATCCCTCCGACTTGACGCATCTATGCCTTCTCTGTCATCAGT
GAGGAAGAACTTGCGTTCATGATCTTGATGGCCGATGGCTGTCCGGCCTC
GCCCCTCGCAGCTCACTTCCTTTCTACCTCTCTCTTTGGTTCCACCAGAC
CTGTCCTACTGCTGTCGACGTGGTCCTACACGTGTTCCCACTTCCATTGT
CGTTGCTGTACGTGAGGACAACTTGCTCGTCGCAAGAGTAGAGAGTTCGT
AACTCTCCATCGACTGTAGCGCTCTAGACATAAACCTCATGATGCTTTGA
AAATTCTAGATACATAGCACTCACGAGGTGTGCTTTGTCGGACCCGCGCG
CATATCCGAGGAGTATGGTACGGTTGTTTCGGAGGGAGCCGAGAACCCAG
TGGTGCTGGCCGCATAGCATGTCAAAAATGGCCACTGTCCGGGTATTTTG
TTTGCCTGAAGCGTGGGCCAACTTTTATTGTGCCGGGACCGAGGTTTTCG
AGTTTATTTACGCGGTCGAAGTCTGCAAATACATGTGTGAAACCCATTTA
TCTGCGGGCCGCACGCTTGTACAATGTTGCACCTCTGAATACATGAAGGA
AAGGAGAAACCAGAGCAGAGGTGTCGTCACCGCGGCAGCAAAGAAAGAAA
TTACCGTGCAGATCTGCTGCCGTGCGGGTTGCTCAACACACCAACGAGAT
TATTGATGATGGTTGCTTTGGGCTTTCATTCCAAAAAACAGCAAATGGAG
TCAAGAGAGCGAAGACGAAAGCAGATACCAATCAACAGATGCAAAGATGC
CAGGGCATCATCATCATGACATTACTTGAAGTGAATCTGTGGCGAGAGGG
CGGGGAGAAGAAGATGTAGAAACATACACGCATCCCAGGGTGTGGGTACT
GCTGCGGTAGCTCGACATGAAAATGCGTGTGTGTGTGTGTGTGTGTGTGT
GATCTCCCTCCTCGCGGTTTCTAAGCTCTCCTTGTCGTCTCGGTGGTGCT
ACTGCATGCTCGTGATTGCCACTAAGCGTTGGTTAAAACGTTGCGAAACT
TCGTACACTTGGAAATCGGAGTGTTCTCTGCTGGTCCTATCCTGCTGTGG
GCGTGTGGTATGCGCCAGTTGCAGCTCCGAGGCTCCCGGCTCATGATGTG
TATTGGTCTGTGAGCTGGTCGGTTCGACTGCGGTGCCCAGAAGAAAGCGG
CGTAATTCCAAATTTACTCGATTATAGACAAGATGCCAAGTAGGCGCCGA
GTTAAATGGAGACAGCTGATTGGCAGACGCCAAGTTAAATCTGTTCCGGC
TTTTTCTGCTGCCAAAACGCATGTCTGGACGGACACGCGTTTCCTCTTGG
AAGGTTGCACTAACGTTGGCTCTACCGCTTGCAACATGACCATTGCAGGG
GAAGATCTACAACTGTGGAGGTGGTGGCTCGTGTGGCACGTGCGCCGTGA
ACGTGAAAGCTGGCGCGCAAAATTGTAGTCCGAAGGGACCCGGCGAGAAG
AAACTTCTCGAGAAGAACAAGAGGCCGGCTTCGTATAGGCTTTCTTGTTG
CACGATGGTTTCGGGACCTGTAACAGTCCAGACGAAGCCGTAA
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Coding sequence (CDS) from alignment at Ecto-sp8_S_contig10978:1187..3879-

>mRNA_Ecto-sp8_S_contig10978.1343.1 ID=mRNA_Ecto-sp8_S_contig10978.1343.1|Name=mRNA_Ecto-sp8_S_contig10978.1343.1|organism=Ectocarpus species8 EcLAC_412|type=CDS|length=480bp|location=Sequence derived from alignment at Ecto-sp8_S_contig10978:1187..3879- (Ectocarpus species8 EcLAC_412)
ATGAAGTCCTTTTTGTTCGTGGCGCTCATCCTTCTTGCAGCTGTGCTGCA
GATATCAAATGGGTTTGTAGTGGCTCCATCGTCATCGCTCAATGTGCGAC
GGCAGTCACACTCTGGTATGCAGATGCTCTTCGGCGGCGGAGGTGCCAAG
AAAAAAGGTGCTGGAGGGATCGTGGTGAAGGTGCAACAGAAGACAGGGTT
CAAGGAAACGGAGCTCACGCTGGCGGGCAAGACGAACCTGAGGAAGGCTC
TGATGGACAACAAAATTGACATCTACCCGCTGCAGGGGAAGATCTACAAC
TGTGGAGGTGGTGGCTCGTGTGGCACGTGCGCCGTGAACGTGAAAGCTGG
CGCGCAAAATTGTAGTCCGAAGGGACCCGGCGAGAAGAAACTTCTCGAGA
AGAACAAGAGGCCGGCTTCGTATAGGCTTTCTTGTTGCACGATGGTTTCG
GGACCTGTAACAGTCCAGACGAAGCCGTAA
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