mRNA_Ecto-sp8_S_contig104282.684.1 (mRNA) Ectocarpus species8 EcLAC_412

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_Ecto-sp8_S_contig104282.684.1
Unique NamemRNA_Ecto-sp8_S_contig104282.684.1
TypemRNA
OrganismEctocarpus species8 EcLAC_412 (Ectocarpus species8 EcLAC_412)
Homology
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BLAST of mRNA_Ecto-sp8_S_contig104282.684.1 vs. uniprot
Match: A0A0U2LGG4_9MAXI (60S ribosomal protein L32 n=5 Tax=Calanoida TaxID=6833 RepID=A0A0U2LGG4_9MAXI)

HSP 1 Score: 222 bits (566), Expect = 8.550e-73
Identity = 109/134 (81.34%), Postives = 123/134 (91.79%), Query Frame = 1 
Query:    1 MALTPASRPKIVKKRVKKFIRHQSDRYDKVKCNWRRPKGIDNRVRRKFKGMYKMPNIGYGSNSTTRHMMPTGFRKVLIHNIKELEVLMMSNKTYAAEIARGVSAKNRKSLIERAAQLAIRVTNPNARIRSEENE 402
            MALTPASRPKIVKKR KKFIRHQSDRYDK+K NWRRPKGIDNRVRRKF+GMYK+P IGYGS   T+HMMP+GFRKVL+HN+KELEVLMM NK + AEIA GVS+KNRK+L+ERA QLAIRVTNP+A++RSEENE
Sbjct:    1 MALTPASRPKIVKKRTKKFIRHQSDRYDKLKQNWRRPKGIDNRVRRKFRGMYKLPGIGYGSAKATKHMMPSGFRKVLVHNVKELEVLMMQNKKFCAEIAHGVSSKNRKNLVERAQQLAIRVTNPSAKVRSEENE 134
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BLAST of mRNA_Ecto-sp8_S_contig104282.684.1 vs. uniprot
Match: A0A0U2US32_ACAPC (60S ribosomal protein L32 n=2 Tax=Acartia pacifica TaxID=335913 RepID=A0A0U2US32_ACAPC)

HSP 1 Score: 218 bits (554), Expect = 5.770e-71
Identity = 107/134 (79.85%), Postives = 122/134 (91.04%), Query Frame = 1 
Query:    1 MALTPASRPKIVKKRVKKFIRHQSDRYDKVKCNWRRPKGIDNRVRRKFKGMYKMPNIGYGSNSTTRHMMPTGFRKVLIHNIKELEVLMMSNKTYAAEIARGVSAKNRKSLIERAAQLAIRVTNPNARIRSEENE 402
            MALTPA+RPKI+KKR K+FIRHQSDRY K+K NWR+PKGIDNRVRRKF+GMYKMP IGYGS   T+HM+PTGFRKVL+HN+KELEVLMM NK + AEIA GVSAKNRK+L+ERA QLAIRVTNP+AR+RSEENE
Sbjct:    1 MALTPANRPKILKKRTKRFIRHQSDRYIKLKPNWRKPKGIDNRVRRKFRGMYKMPGIGYGSAKATKHMLPTGFRKVLVHNVKELEVLMMQNKKFCAEIAHGVSAKNRKTLVERAQQLAIRVTNPHARLRSEENE 134
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BLAST of mRNA_Ecto-sp8_S_contig104282.684.1 vs. uniprot
Match: A0A0U2V7Q5_9MAXI (60S ribosomal protein L32 n=1 Tax=Centropages tenuiremis TaxID=544689 RepID=A0A0U2V7Q5_9MAXI)

HSP 1 Score: 216 bits (549), Expect = 3.340e-70
Identity = 107/134 (79.85%), Postives = 121/134 (90.30%), Query Frame = 1 
Query:    1 MALTPASRPKIVKKRVKKFIRHQSDRYDKVKCNWRRPKGIDNRVRRKFKGMYKMPNIGYGSNSTTRHMMPTGFRKVLIHNIKELEVLMMSNKTYAAEIARGVSAKNRKSLIERAAQLAIRVTNPNARIRSEENE 402
            MALTPASRPKIVKKR KKFIRHQSD Y K+K NWRRPKGIDNRVRRKF+GMYK+P IGYGSN  TRHM+PTGFRKVL++N+KELEVLMM NK Y AEIA GVS+KNRK+++ERA QLAIRVTN +A++RSEENE
Sbjct:    1 MALTPASRPKIVKKRTKKFIRHQSDWYHKLKTNWRRPKGIDNRVRRKFRGMYKLPGIGYGSNKATRHMLPTGFRKVLVNNLKELEVLMMQNKKYCAEIAHGVSSKNRKAIVERAQQLAIRVTNGSAKLRSEENE 134
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BLAST of mRNA_Ecto-sp8_S_contig104282.684.1 vs. uniprot
Match: A0A553NCB5_TIGCA (60S ribosomal protein L32 n=1 Tax=Tigriopus californicus TaxID=6832 RepID=A0A553NCB5_TIGCA)

HSP 1 Score: 216 bits (549), Expect = 3.340e-70
Identity = 107/134 (79.85%), Postives = 121/134 (90.30%), Query Frame = 1 
Query:    1 MALTPASRPKIVKKRVKKFIRHQSDRYDKVKCNWRRPKGIDNRVRRKFKGMYKMPNIGYGSNSTTRHMMPTGFRKVLIHNIKELEVLMMSNKTYAAEIARGVSAKNRKSLIERAAQLAIRVTNPNARIRSEENE 402
            MA+TPA+ PKIVKKR K+F+RHQSDRY KV+ +WR+PKGIDNRVRRKFKGMY MPNIGYGSN  TRHM+PTGFRKVLI+N+KELEVLMM NK + AEIA GVSAKNRK+L+ERA QL IRVTNPNAR+RSEENE
Sbjct:    1 MAITPAAHPKIVKKRTKRFLRHQSDRYVKVRQSWRKPKGIDNRVRRKFKGMYLMPNIGYGSNKNTRHMLPTGFRKVLINNMKELEVLMMQNKKFCAEIAHGVSAKNRKALVERAQQLCIRVTNPNARLRSEENE 134
[Close]
BLAST of mRNA_Ecto-sp8_S_contig104282.684.1 vs. uniprot
Match: A0A0U2T7P6_9MAXI (60S ribosomal protein L32 n=2 Tax=Pseudodiaptomus poplesia TaxID=213370 RepID=A0A0U2T7P6_9MAXI)

HSP 1 Score: 212 bits (540), Expect = 7.850e-69
Identity = 105/134 (78.36%), Postives = 120/134 (89.55%), Query Frame = 1 
Query:    1 MALTPASRPKIVKKRVKKFIRHQSDRYDKVKCNWRRPKGIDNRVRRKFKGMYKMPNIGYGSNSTTRHMMPTGFRKVLIHNIKELEVLMMSNKTYAAEIARGVSAKNRKSLIERAAQLAIRVTNPNARIRSEENE 402
            MALTPA RPKIVKKR K+F RHQSDRY K+K NWR+PKGIDNRVRRKFKGMY MPNIGYGS   TRHM+P+GFRKVLIHN+KELEVLMM NK + AEIA GVSAKNRK+++ERA QLAIR+TNP+A++R+EENE
Sbjct:    1 MALTPAERPKIVKKRTKQFTRHQSDRYVKLKRNWRKPKGIDNRVRRKFKGMYLMPNIGYGSAKATRHMLPSGFRKVLIHNMKELEVLMMQNKKFCAEIAHGVSAKNRKTIVERAQQLAIRITNPSAKMRTEENE 134
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BLAST of mRNA_Ecto-sp8_S_contig104282.684.1 vs. uniprot
Match: A0A6Q2XWB9_ESOLU (60S ribosomal protein L32 n=4 Tax=Protacanthopterygii TaxID=41705 RepID=A0A6Q2XWB9_ESOLU)

HSP 1 Score: 211 bits (536), Expect = 3.300e-68
Identity = 104/133 (78.20%), Postives = 120/133 (90.23%), Query Frame = 1 
Query:    4 ALTPASRPKIVKKRVKKFIRHQSDRYDKVKCNWRRPKGIDNRVRRKFKGMYKMPNIGYGSNSTTRHMMPTGFRKVLIHNIKELEVLMMSNKTYAAEIARGVSAKNRKSLIERAAQLAIRVTNPNARIRSEENE 402
            AL P  RPKIVKKRVKKFIRHQSDRY KVK NWR+P+GIDNRVRR+FKG   MPNIGYGSN+ T+HM+P+GFRK L+HNI+ELEVLMMSNKT+AAEIA  VS+KNRK ++ERAAQLAI++TNPNAR+RSEENE
Sbjct:    3 ALRPLQRPKIVKKRVKKFIRHQSDRYVKVKQNWRKPRGIDNRVRRRFKGQMLMPNIGYGSNNNTKHMLPSGFRKFLVHNIRELEVLMMSNKTHAAEIAHNVSSKNRKLIVERAAQLAIKITNPNARLRSEENE 135
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BLAST of mRNA_Ecto-sp8_S_contig104282.684.1 vs. uniprot
Match: A0A8C4XF28_ERPCA (Ribosomal protein L32 n=3 Tax=Euteleostomi TaxID=117571 RepID=A0A8C4XF28_ERPCA)

HSP 1 Score: 207 bits (528), Expect = 5.470e-67
Identity = 101/133 (75.94%), Postives = 119/133 (89.47%), Query Frame = 1 
Query:    4 ALTPASRPKIVKKRVKKFIRHQSDRYDKVKCNWRRPKGIDNRVRRKFKGMYKMPNIGYGSNSTTRHMMPTGFRKVLIHNIKELEVLMMSNKTYAAEIARGVSAKNRKSLIERAAQLAIRVTNPNARIRSEENE 402
            AL P ++PKIVKKR KKFIRHQSDRY K+K NWR+P+GIDNRVRR+FKG   MPNIGYGSN  T+HM+P+GFRK L+HNIKELEVL+MSNK+Y AEIA  VS+KNRK+++ERAAQLAI+VTNPNAR+RSEENE
Sbjct:    3 ALRPLTKPKIVKKRTKKFIRHQSDRYVKIKKNWRKPRGIDNRVRRRFKGQILMPNIGYGSNKKTKHMLPSGFRKFLVHNIKELEVLLMSNKSYCAEIAHNVSSKNRKAIVERAAQLAIKVTNPNARLRSEENE 135
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BLAST of mRNA_Ecto-sp8_S_contig104282.684.1 vs. uniprot
Match: A0A1S3HVD9_LINUN (60S ribosomal protein L32 n=1 Tax=Lingula unguis TaxID=7574 RepID=A0A1S3HVD9_LINUN)

HSP 1 Score: 207 bits (527), Expect = 7.520e-67
Identity = 103/134 (76.87%), Postives = 116/134 (86.57%), Query Frame = 1 
Query:    1 MALTPASRPKIVKKRVKKFIRHQSDRYDKVKCNWRRPKGIDNRVRRKFKGMYKMPNIGYGSNSTTRHMMPTGFRKVLIHNIKELEVLMMSNKTYAAEIARGVSAKNRKSLIERAAQLAIRVTNPNARIRSEENE 402
            MAL P  +PKIVKKR KKFIRHQSDRY K+K NWR+PKGIDNRVRR+FKG Y MPNIGYGSN  T+HMMP GF+K L+HN+ ELEVLMM NKT+AAEIA  VSAK RKS++ERA QLAI+VTNPNAR+RSEENE
Sbjct:    1 MALRPLCKPKIVKKRTKKFIRHQSDRYLKLKPNWRKPKGIDNRVRRRFKGQYLMPNIGYGSNKKTKHMMPDGFKKFLVHNVAELEVLMMQNKTFAAEIASSVSAKKRKSIVERAQQLAIKVTNPNARLRSEENE 134
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BLAST of mRNA_Ecto-sp8_S_contig104282.684.1 vs. uniprot
Match: A0A6M2DKK4_9NEOP (60S ribosomal protein L32 n=3 Tax=Pulicidae TaxID=7511 RepID=A0A6M2DKK4_9NEOP)

HSP 1 Score: 207 bits (527), Expect = 7.520e-67
Identity = 101/134 (75.37%), Postives = 116/134 (86.57%), Query Frame = 1 
Query:    1 MALTPASRPKIVKKRVKKFIRHQSDRYDKVKCNWRRPKGIDNRVRRKFKGMYKMPNIGYGSNSTTRHMMPTGFRKVLIHNIKELEVLMMSNKTYAAEIARGVSAKNRKSLIERAAQLAIRVTNPNARIRSEENE 402
            M++ P  RPKIVKKR KKFIRHQSDRYDK+K NWR+PKGIDNRVRR+FKG Y MPNIGYGSNS TRHM+PTGF KVL+HN +ELE+LMM N+TY AEIA GVS+K RK + ERA QL+IRVTN NAR+RS+ENE
Sbjct:    1 MSIRPVYRPKIVKKRTKKFIRHQSDRYDKLKRNWRKPKGIDNRVRRRFKGQYLMPNIGYGSNSKTRHMLPTGFHKVLVHNTRELEILMMQNRTYCAEIAHGVSSKKRKEITERAKQLSIRVTNGNARLRSQENE 134
[Close]
BLAST of mRNA_Ecto-sp8_S_contig104282.684.1 vs. uniprot
Match: A0A091IU71_EGRGA (60S ribosomal protein L32 n=65 Tax=Aves TaxID=8782 RepID=A0A091IU71_EGRGA)

HSP 1 Score: 207 bits (527), Expect = 7.770e-67
Identity = 101/133 (75.94%), Postives = 117/133 (87.97%), Query Frame = 1 
Query:    4 ALTPASRPKIVKKRVKKFIRHQSDRYDKVKCNWRRPKGIDNRVRRKFKGMYKMPNIGYGSNSTTRHMMPTGFRKVLIHNIKELEVLMMSNKTYAAEIARGVSAKNRKSLIERAAQLAIRVTNPNARIRSEENE 402
            AL P  +PKIVKKR KKFIRHQSDRY K+K NWR+P+GIDNRVRR+FKG   MPNIGYGSN  T+HM+PTGFRK L+HN+KELEVLMMSNK+Y AEIA  VS+KNRK ++ERAAQLAI++TNPNAR+RSEENE
Sbjct:    3 ALRPLVKPKIVKKRTKKFIRHQSDRYVKIKRNWRKPRGIDNRVRRRFKGQILMPNIGYGSNKKTKHMLPTGFRKFLVHNVKELEVLMMSNKSYCAEIAHNVSSKNRKIIVERAAQLAIKITNPNARLRSEENE 135
The following BLAST results are available for this feature:
BLAST of mRNA_Ecto-sp8_S_contig104282.684.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A0U2LGG4_9MAXI8.550e-7381.3460S ribosomal protein L32 n=5 Tax=Calanoida TaxID=... [more]
A0A0U2US32_ACAPC5.770e-7179.8560S ribosomal protein L32 n=2 Tax=Acartia pacifica... [more]
A0A0U2V7Q5_9MAXI3.340e-7079.8560S ribosomal protein L32 n=1 Tax=Centropages tenu... [more]
A0A553NCB5_TIGCA3.340e-7079.8560S ribosomal protein L32 n=1 Tax=Tigriopus califo... [more]
A0A0U2T7P6_9MAXI7.850e-6978.3660S ribosomal protein L32 n=2 Tax=Pseudodiaptomus ... [more]
A0A6Q2XWB9_ESOLU3.300e-6878.2060S ribosomal protein L32 n=4 Tax=Protacanthoptery... [more]
A0A8C4XF28_ERPCA5.470e-6775.94Ribosomal protein L32 n=3 Tax=Euteleostomi TaxID=1... [more]
A0A1S3HVD9_LINUN7.520e-6776.8760S ribosomal protein L32 n=1 Tax=Lingula unguis T... [more]
A0A6M2DKK4_9NEOP7.520e-6775.3760S ribosomal protein L32 n=3 Tax=Pulicidae TaxID=... [more]
A0A091IU71_EGRGA7.770e-6775.9460S ribosomal protein L32 n=65 Tax=Aves TaxID=8782... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
Ecto-sp8_S_contig104282contigEcto-sp8_S_contig104282:55..459 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Ectocarpus species8 EcLAC_412 OGS1.02022-07-08
Properties
Property NameValue
Taxonomic scopeMetazoa
Stop1
Start1
Seed ortholog score212.2
Seed ortholog evalue1.5e-52
Seed eggNOG ortholog8010.XP_010862027.1
Preferred nameRPL32
Model size405
KEGG koko:K02912
KEGG Pathwayko03010,map03010
KEGG ModuleM00177,M00179
Hectar predicted targeting categoryother localisation
GOsGO:0000184,GO:0000956,GO:0001889,GO:0002181,GO:0003674,GO:0003735,GO:0005198,GO:0005575,GO:0005622,GO:0005623,GO:0005634,GO:0005730,GO:0005737,GO:0005783,GO:0005789,GO:0005791,GO:0005829,GO:0005840,GO:0005844,GO:0006139,GO:0006401,GO:0006402,GO:0006412,GO:0006413,GO:0006518,GO:0006605,GO:0006612,GO:0006613,GO:0006614,GO:0006725,GO:0006807,GO:0006810,GO:0006886,GO:0007275,GO:0008104,GO:0008150,GO:0008152,GO:0009056,GO:0009057,GO:0009058,GO:0009059,GO:0009410,GO:0009719,GO:0009725,GO:0009892,GO:0009987,GO:0010033,GO:0010467,GO:0010468,GO:0010605,GO:0010629,GO:0012505,GO:0014070,GO:0015031,GO:0015833,GO:0015934,GO:0016020,GO:0016070,GO:0016071,GO:0019222,GO:0019439,GO:0019538,GO:0022625,GO:0022626,GO:0030867,GO:0031090,GO:0031099,GO:0031100,GO:0031960,GO:0031974,GO:0031981,GO:0031984,GO:0032501,GO:0032502,GO:0032870,GO:0032991,GO:0033036,GO:0033365,GO:0033993,GO:0034613,GO:0034641,GO:0034645,GO:0034655,GO:0035690,GO:0042175,GO:0042221,GO:0042493,GO:0042788,GO:0042886,GO:0043043,GO:0043170,GO:0043226,GO:0043227,GO:0043228,GO:0043229,GO:0043231,GO:0043232,GO:0043233,GO:0043603,GO:0043604,GO:0044237,GO:0044238,GO:0044248,GO:0044249,GO:0044260,GO:0044265,GO:0044267,GO:0044270,GO:0044271,GO:0044391,GO:0044422,GO:0044424,GO:0044425,GO:0044428,GO:0044432,GO:0044444,GO:0044445,GO:0044446,GO:0044464,GO:0045047,GO:0045184,GO:0046483,GO:0046700,GO:0046907,GO:0048513,GO:0048519,GO:0048545,GO:0048731,GO:0048732,GO:0048856,GO:0050789,GO:0050896,GO:0051179,GO:0051234,GO:0051384,GO:0051641,GO:0051649,GO:0051716,GO:0060255,GO:0061008,GO:0065007,GO:0070013,GO:0070727,GO:0070887,GO:0070972,GO:0071310,GO:0071383,GO:0071384,GO:0071385,GO:0071396,GO:0071407,GO:0071466,GO:0071495,GO:0071548,GO:0071549,GO:0071702,GO:0071704,GO:0071705,GO:0072594,GO:0072599,GO:0072657,GO:0090150,GO:0090304,GO:0097327,GO:0097421,GO:0098552,GO:0098554,GO:0098556,GO:0098562,GO:0098588,GO:0098827,GO:1901360,GO:1901361,GO:1901564,GO:1901566,GO:1901575,GO:1901576,GO:1901654,GO:1901655,GO:1901700,GO:1901701,GO:1990904
Exons1
EggNOG free text desc.Ribosomal protein L32
EggNOG OGs3A0DJ@33154,3BPE5@33208,3D6EA@33213,48E1J@7711,49ASQ@7742,4A2RB@7898,COG1717@1,KOG0878@2759
Cds size405
COG Functional cat.J
Best tax levelActinopterygii
Best eggNOG OGNA|NA|NA
BRITEbr01610,ko00000,ko00001,ko00002,ko03011
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1681143141.3980355-CDS-Ecto-sp8_S_contig104282:54..4591681143141.3980355-CDS-Ecto-sp8_S_contig104282:54..459Ectocarpus species8 EcLAC_412CDSEcto-sp8_S_contig104282 55..459 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_Ecto-sp8_S_contig104282.684.1prot_Ecto-sp8_S_contig104282.684.1Ectocarpus species8 EcLAC_412polypeptideEcto-sp8_S_contig104282 55..459 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_Ecto-sp8_S_contig104282.684.1

>prot_Ecto-sp8_S_contig104282.684.1 ID=prot_Ecto-sp8_S_contig104282.684.1|Name=mRNA_Ecto-sp8_S_contig104282.684.1|organism=Ectocarpus species8 EcLAC_412|type=polypeptide|length=135bp
MALTPASRPKIVKKRVKKFIRHQSDRYDKVKCNWRRPKGIDNRVRRKFKG
MYKMPNIGYGSNSTTRHMMPTGFRKVLIHNIKELEVLMMSNKTYAAEIAR
GVSAKNRKSLIERAAQLAIRVTNPNARIRSEENE*
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mRNA from alignment at Ecto-sp8_S_contig104282:55..459-

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_Ecto-sp8_S_contig104282.684.1 ID=mRNA_Ecto-sp8_S_contig104282.684.1|Name=mRNA_Ecto-sp8_S_contig104282.684.1|organism=Ectocarpus species8 EcLAC_412|type=mRNA|length=405bp|location=Sequence derived from alignment at Ecto-sp8_S_contig104282:55..459- (Ectocarpus species8 EcLAC_412)
ATGGCTCTGACCCCGGCCTCCAGGCCAAAGATTGTCAAGAAAAGGGTGAA
GAAGTTCATCCGCCACCAGTCTGACCGGTATGACAAGGTCAAGTGTAACT
GGAGGAGACCAAAGGGTATTGACAACAGAGTCCGCCGTAAGTTCAAGGGG
ATGTACAAGATGCCCAACATTGGTTACGGAAGCAACAGCACCACCCGCCA
CATGATGCCCACTGGCTTCAGGAAGGTCCTGATCCACAACATCAAGGAGC
TGGAGGTCTTGATGATGTCCAACAAGACATACGCTGCCGAGATCGCCCGT
GGAGTCTCCGCAAAGAACAGGAAGTCCCTGATCGAAAGAGCTGCCCAGTT
GGCTATTCGCGTCACCAATCCTAATGCAAGAATTCGCTCAGAAGAGAATG
AATAA
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Coding sequence (CDS) from alignment at Ecto-sp8_S_contig104282:55..459-

>mRNA_Ecto-sp8_S_contig104282.684.1 ID=mRNA_Ecto-sp8_S_contig104282.684.1|Name=mRNA_Ecto-sp8_S_contig104282.684.1|organism=Ectocarpus species8 EcLAC_412|type=CDS|length=405bp|location=Sequence derived from alignment at Ecto-sp8_S_contig104282:55..459- (Ectocarpus species8 EcLAC_412)
ATGGCTCTGACCCCGGCCTCCAGGCCAAAGATTGTCAAGAAAAGGGTGAA
GAAGTTCATCCGCCACCAGTCTGACCGGTATGACAAGGTCAAGTGTAACT
GGAGGAGACCAAAGGGTATTGACAACAGAGTCCGCCGTAAGTTCAAGGGG
ATGTACAAGATGCCCAACATTGGTTACGGAAGCAACAGCACCACCCGCCA
CATGATGCCCACTGGCTTCAGGAAGGTCCTGATCCACAACATCAAGGAGC
TGGAGGTCTTGATGATGTCCAACAAGACATACGCTGCCGAGATCGCCCGT
GGAGTCTCCGCAAAGAACAGGAAGTCCCTGATCGAAAGAGCTGCCCAGTT
GGCTATTCGCGTCACCAATCCTAATGCAAGAATTCGCTCAGAAGAGAATG
AATAA
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