Ec-28_003220.2 (polypeptide) Ectocarpus species7 Ec32 male_plus_femaleSDR

You are viewing a polypeptide, more information available on the corresponding mRNA page

Overview
NameEc-28_003220.2
Unique NameEc-28_003220.2
Typepolypeptide
OrganismEctocarpus species7 Ec32 male_plus_femaleSDR (Ectocarpus species7 Ec32 male_plus_femaleSDR)
Sequence length250
Homology
BLAST of Ec-28_003220.2 vs. uniprot
Match: A0A6H5KR44_9PHAE (Lipoyl-binding domain-containing protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5KR44_9PHAE)

HSP 1 Score: 347 bits (891), Expect = 1.240e-118
Identity = 216/249 (86.75%), Postives = 221/249 (88.76%), Query Frame = 0
Query:    1 MIACRRTSTFAARVLATQCQGTRQAASRRSAELVVKRRSLSSLPDHIVVGMPALSPTMTHGNIGSWGKQEGDEIAAGDVVCEVETDKATVDFDAQDDSFMAKHLVEAGTQDIAVGTPIFVTVDDADSVAAFKDFEAEAVQGEPEAAAXXXXXXXXXXXXXXXXXXXXXXXPAPEAAPAPXXXXXXXXXXXXTTAAPVERPPAPVSSPELVFKRDWGSGVRGSALAHRMSAEQKKYVERFGSTLQQPLDP 249
            M+ACRRTST AA VLATQC+GTRQ ASRRSAELVV  RSLSSLPDHIVVGMPALSPTMTHGNIGSWGKQEGDEIAAGDVVCEVETDKATVDFDAQDDSFMAKHLVEAGTQDIAVGTPIFVTVDDADSVAAFKDFEAEAVQGEPEAAA         XXXXXXX   XXXX  PEA    XXXXXXXXXXXX  +  VE+PPAPVSS ELVFKRDWGSGVRGSALAHRMSAEQKKYV+RFGSTLQQPLDP
Sbjct:    1 MMACRRTSTLAASVLATQCRGTRQVASRRSAELVVNTRSLSSLPDHIVVGMPALSPTMTHGNIGSWGKQEGDEIAAGDVVCEVETDKATVDFDAQDDSFMAKHLVEAGTQDIAVGTPIFVTVDDADSVAAFKDFEAEAVQGEPEAAAPAAEAAPTPXXXXXXXAAKXXXXXXPEAXXXXXXXXXXXXXXXXXXS--VEQPPAPVSSQELVFKRDWGSGVRGSALAHRMSAEQKKYVKRFGSTLQQPLDP 247          
BLAST of Ec-28_003220.2 vs. uniprot
Match: D8LTQ3_ECTSI (Lipoyl-binding domain-containing protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LTQ3_ECTSI)

HSP 1 Score: 305 bits (780), Expect = 3.860e-102
Identity = 197/197 (100.00%), Postives = 197/197 (100.00%), Query Frame = 0
Query:   53 ALSPTMTHGNIGSWGKQEGDEIAAGDVVCEVETDKATVDFDAQDDSFMAKHLVEAGTQDIAVGTPIFVTVDDADSVAAFKDFEAEAVQGEPEAAAXXXXXXXXXXXXXXXXXXXXXXXPAPEAAPAPXXXXXXXXXXXXTTAAPVERPPAPVSSPELVFKRDWGSGVRGSALAHRMSAEQKKYVERFGSTLQQPLDP 249
            ALSPTMTHGNIGSWGKQEGDEIAAGDVVCEVETDKATVDFDAQDDSFMAKHLVEAGTQDIAVGTPIFVTVDDADSVAAFKDFEAEAVQGEPEAAAXXXXXXXXXXXXXXXXXXXXXXXPAPEAAPAPXXXXXXXXXXXXTTAAPVERPPAPVSSPELVFKRDWGSGVRGSALAHRMSAEQKKYVERFGSTLQQPLDP
Sbjct:   25 ALSPTMTHGNIGSWGKQEGDEIAAGDVVCEVETDKATVDFDAQDDSFMAKHLVEAGTQDIAVGTPIFVTVDDADSVAAFKDFEAEAVQGEPEAAAXXXXXXXXXXXXXXXXXXXXXXXPAPEAAPAPXXXXXXXXXXXXTTAAPVERPPAPVSSPELVFKRDWGSGVRGSALAHRMSAEQKKYVERFGSTLQQPLDP 221          
BLAST of Ec-28_003220.2 vs. uniprot
Match: A0A836CM78_9STRA (Putative dihydrolipoyllysineresidue acetyltransferase component of pyruvate dehydrogenase complex n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A836CM78_9STRA)

HSP 1 Score: 152 bits (385), Expect = 4.540e-42
Identity = 108/219 (49.32%), Postives = 133/219 (60.73%), Query Frame = 0
Query:   38 RSLSSLPDHIVVGMPALSPTMTHGNIGSWGKQEGDEIAAGDVVCEVETDKATVDFDAQDDSFMAKHLVEAGTQDIAVGTPIFVTVDDADSVAAFKDFEAEAVQGEPE--------AAAXXXXXXXXXXXXXXXXXXXXXXXPAPEAAPAPXXXXXXXXXXXXTTAAPV---ERPPAPVSSPELVFKRDWGSGVRGSALAHRMSAEQKKYVERFGSTLQQ 245
            R++++LP H +VGMPALSPTM HGN+  W K  GDEIAAGD++C+VETDKATVDF+AQD+ F+AK LV  G Q I VG PI +TV+D DSVAAF+ +EA A +  P         AAA       XXX               P+  P PXXXXXXXXXX   T A     + P AP  +P      +WG  V  S L +R+S EQK Y  RFG+TLQ+
Sbjct:   32 RAMATLPSHTLVGMPALSPTMEHGNLAKWLKAAGDEIAAGDIICQVETDKATVDFEAQDEGFVAKLLVPEGAQSITVGDPILITVEDKDSVAAFESYEAPATKEAPXXXXXXXXVAAATPPAVSPXXXAEAPKVEAVK-----PKTMPPPXXXXXXXXXXAKATEAAKSAQKAPDAPAGAPTASV--NWGQRVSRSPLYYRLSQEQKLYSSRFGATLQE 243          
BLAST of Ec-28_003220.2 vs. uniprot
Match: A0A485LRT4_9STRA (Aste57867_24277 protein n=1 Tax=Aphanomyces stellatus TaxID=120398 RepID=A0A485LRT4_9STRA)

HSP 1 Score: 142 bits (357), Expect = 7.280e-38
Identity = 97/245 (39.59%), Postives = 124/245 (50.61%), Query Frame = 0
Query:   24 QAASRRSAELVVKRRSLSSLPDHIVVGMPALSPTMTHGNIGSWGKQEGDEIAAGDVVCEVETDKATVDFDAQDDSFMAKHLVEAGTQDIAVGTPIFVTVDDADSVAAFKDFEAEAVQGEPEAAAXXXXXXXXXXXXXXXXXXXXXXXPAPEAAPAPXXXXXXXXXXXXTTAAPVERPPAPVSSP--------------------ELVFKRDWGSGVRGSALAHRMSAEQKKYVERFGSTLQQPLD 248
            +AAS RS+   +  R  +SLP H VVG+PALSPTM  GN+  W  +EGD I+AGDV+C++ETDKA VD++AQDD F+A+ LV  G + I VG PI VTVD AD VAAFKDF A+ V   P  A                        PA +A   P              A P    PAPV++P                     L     WG GV  SA++H ++  QK YV  +GST   P++
Sbjct:   11 RAASMRSSSFAL--RHFASLPAHEVVGLPALSPTMEQGNLAKWNLKEGDAISAGDVICQIETDKAIVDYEAQDDMFLARILVPEGAEGIVVGQPIMVTVDSADDVAAFKDFVAD-VAATPAPAPKSPEEIPPKKHEVKVD-------PATQAPVVPMDFLVTSQTPQKIVAGPAI--PAPVATPATXXXXXXXXXXXXXXXXXANLASFSKWGLGVNKSAISHSLAQRQKAYVALYGSTGTFPIE 243          
BLAST of Ec-28_003220.2 vs. uniprot
Match: A0A0P1AE60_PLAHL (Pyruvate dehydrogenase complex dihydrolipoamide acetyltransferase n=1 Tax=Plasmopara halstedii TaxID=4781 RepID=A0A0P1AE60_PLAHL)

HSP 1 Score: 141 bits (355), Expect = 1.020e-37
Identity = 78/210 (37.14%), Postives = 116/210 (55.24%), Query Frame = 0
Query:   38 RSLSSLPDHIVVGMPALSPTMTHGNIGSWGKQEGDEIAAGDVVCEVETDKATVDFDAQDDSFMAKHLVEAGTQDIAVGTPIFVTVDDADSVAAFKDFEAEAVQGEPEAAAXXXXXXXXXXXXXXXXXXXXXXXPAPEAAPAPXXXXXXXXXXXXTTAAPVERPPAPVSSPELVFKRDWGSGVRGSALAHRMSAEQKKYVERFGSTLQQPL 247
            R  SS P H +VG+P+LSPTM  GN+  W  +EGD I AGD+VCE+ETDKA VD++A DD F+AK L+  GT++I VG P+ V V+D +SVAAFKDF+ +      EA+                        P+ +A+P              +T     +  +PVS+   VF+  WG G++ SA++  +  +Q+ Y+ ++G T   P+
Sbjct:   24 RGFSSYPPHDIVGLPSLSPTMETGNMSKWNTKEGDAITAGDIVCEIETDKAVVDYEATDDMFLAKILIPEGTENIPVGQPMMVVVEDQESVAAFKDFKLD------EASVAPATPVSSKKPSNKSRSEPAMPQPSQKASPPMEVSPPTKTIEHTSTVLTNAKASSPVSATTTVFEEKWGFGIKKSAISTSLLKKQQLYIAQYGVTGMTPV 227          
BLAST of Ec-28_003220.2 vs. uniprot
Match: V9ET80_PHYPR (Acetyltransferase component of pyruvate dehydrogenase complex n=12 Tax=Phytophthora TaxID=4783 RepID=V9ET80_PHYPR)

HSP 1 Score: 146 bits (369), Expect = 1.610e-37
Identity = 76/114 (66.67%), Postives = 92/114 (80.70%), Query Frame = 0
Query:   25 AASRRSAELVVKRRSLSSLPDHIVVGMPALSPTMTHGNIGSWGKQEGDEIAAGDVVCEVETDKATVDFDAQDDSFMAKHLVEAGTQDIAVGTPIFVTVDDADSVAAFKDFEAEA 138
            AA+RR A + +  RS SSLPDH VVG+PALSPTM  G I  W KQEGD+I+AGDVVCEVETDKA VD++A DDS++AK LV+AG+ +IAVG PIFVTV + + VAAFKDF A+A
Sbjct:   27 AAARREASMAMSARSFSSLPDHEVVGLPALSPTMEVGTIAKWNKQEGDQISAGDVVCEVETDKAVVDYEATDDSYLAKILVQAGSGEIAVGQPIFVTVMEEEDVAAFKDFSADA 140          
BLAST of Ec-28_003220.2 vs. uniprot
Match: A0A8K1FAL3_PYTOL (Uncharacterized protein n=1 Tax=Pythium oligandrum TaxID=41045 RepID=A0A8K1FAL3_PYTOL)

HSP 1 Score: 139 bits (350), Expect = 4.460e-37
Identity = 95/225 (42.22%), Postives = 127/225 (56.44%), Query Frame = 0
Query:   23 RQAASRRSAELVVKRRSLSSLPDHIVVGMPALSPTMTHGNIGSWGKQEGDEIAAGDVVCEVETDKATVDFDAQDDSFMAKHLVEAGTQDIAVGTPIFVTVDDADSVAAFKDFEAEAVQGEPEAAAXXXXXXXXXXXXXXXXXXXXXXXPAPEAAPAPXXXXXXXXXXXXTTAAPVERPPAPVSSPELVFKRDWGSGVRGSALAHRMSAEQKKYVERFGSTLQQPL 247
            R A+S R + L++  R  +S P H VVG+PALSPTM  GN+ SW  +EGD+I+AGD++C++ETDKA VDF+AQDD F+AK LV  GT++I+VG PI VT ++ D VAAFKDF+ EA +    AA                        PAP A PAP    XXXXXXXX                   F   WG G + S L+  +  +Q+ Y++ +G T   P+
Sbjct:    8 RSASSARRSALLL--RGFASYPAHEVVGLPALSPTMEQGNLASWRLKEGDKISAGDIICQIETDKAVVDFEAQDDMFLAKILVPEGTENISVGQPIMVTCEEEDDVAAFKDFKVEASEVAAPAAKAPEEQQPKKEEAKA---------PAPAATPAPAAAKXXXXXXXXXXXXXXXXXXXXXXXXTAKFAEKWGHGSKKSPLSFSLVKKQQAYLDLYGLTGTTPV 221          
BLAST of Ec-28_003220.2 vs. uniprot
Match: A0A425C5Z6_9STRA (Acetyltransferase component of pyruvate dehydrogenase complex n=2 Tax=Peronospora effusa TaxID=542832 RepID=A0A425C5Z6_9STRA)

HSP 1 Score: 142 bits (359), Expect = 4.330e-36
Identity = 74/120 (61.67%), Postives = 91/120 (75.83%), Query Frame = 0
Query:   28 RRSAELVVKRRSLSSLPDHIVVGMPALSPTMTHGNIGSWGKQEGDEIAAGDVVCEVETDKATVDFDAQDDSFMAKHLVEAGTQDIAVGTPIFVTVDDADSVAAFKDFEAEAVQGEPEAAA 147
            R  A +V+  R+ SSLPDH VVG+PALSPTM  G I  W KQEGD I+AGDVVCEVETDKA VDF+A DDS++AK LV++G+ ++AVG PIFVTV + + +AAFKDF A+A   E  A A
Sbjct:   30 RHEASVVLSARTFSSLPDHEVVGLPALSPTMEVGTIAKWNKQEGDPISAGDVVCEVETDKAVVDFEATDDSYLAKILVQSGSGEVAVGKPIFVTVMEEEDIAAFKDFSADATPDENAAPA 149          
BLAST of Ec-28_003220.2 vs. uniprot
Match: A0A0W8DVI9_PHYNI (Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex n=14 Tax=Phytophthora TaxID=4783 RepID=A0A0W8DVI9_PHYNI)

HSP 1 Score: 135 bits (341), Expect = 7.890e-36
Identity = 80/205 (39.02%), Postives = 110/205 (53.66%), Query Frame = 0
Query:   38 RSLSSLPDHIVVGMPALSPTMTHGNIGSWGKQEGDEIAAGDVVCEVETDKATVDFDAQDDSFMAKHLVEAGTQDIAVGTPIFVTVDDADSVAAFKDFEAEAVQGEPEAAAXXXXXXXXXXXXXXXXXXXXXXXPAPEAAPAPXXXXXXXXXXXXTTAAPVERPPAPVSSPELVFKRDWGSGVRGSALAHRMSAEQKKYVERFGST 242
            RS SS PDH V+G+P+LSPTM  GN+  W  +EGD I+AGD+VCE+ETDKA VD++A DD ++AK L+  G ++I VG P+ V VD+ +SVAAFKDF+ E     P A A                       P  E  P                A+      AP SS   VF+  WG G++ SA++  +  +Q+ Y+  +G T
Sbjct:   24 RSFSSYPDHEVIGLPSLSPTMETGNMSKWNMKEGDAISAGDIVCEIETDKAVVDYEATDDMYLAKILIPEGAENIPVGQPMMVVVDEEESVAAFKDFKLEDAPSAPAAPALSAEEKP----------------PKKEDVPVDAKTHEPVVPQDFLAAS------APTSS--AVFEEKWGLGIKKSAISTSLIKKQQAYIALYGIT 204          
BLAST of Ec-28_003220.2 vs. uniprot
Match: A0A1V9YB35_9STRA (Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex n=1 Tax=Achlya hypogyna TaxID=1202772 RepID=A0A1V9YB35_9STRA)

HSP 1 Score: 136 bits (343), Expect = 7.980e-36
Identity = 89/247 (36.03%), Postives = 126/247 (51.01%), Query Frame = 0
Query:   28 RRSAELV---VKRRSLSSLPDHIVVGMPALSPTMTHGNIGSWGKQEGDEIAAGDVVCEVETDKATVDFDAQDDSFMAKHLVEAGTQDIAVGTPIFVTVDDADSVAAFKDFEAEA--------------------VQGEPEAAAXXXXXXXXXXXXXXXXXXXXXXXPAPEAAPAPXXXXXXXXXXXXTTAAP--VERPPAPVSSPELVFKRDWGSGVRGSALAHRMSAEQKKYVERFGSTLQQPLDP 249
            RR+A L    ++RR  ++ P H +VG+PALSPTM  GN+  W  +EGD IAAGD++C++ETDKA VD++AQDD F+A+ LV  GT+ I VG PI +TVDDA +V AF DF  +A                    V+ +P   A                       PAP AAP               T AP      PA  S+    F++ WG G++ S +++ +   QK Y+  +G+T   P++P
Sbjct:    4 RRAARLTTRALRRRGFATYPSHELVGLPALSPTMEQGNLAKWNMKEGDAIAAGDIICQIETDKAIVDYEAQDDMFLARILVPEGTEGITVGQPIMITVDDAANVGAFADFVVDAAAVPTPAPKSPEEIPPKKEEVKVDPATNAPVAPADFLVTSQVPQKTVPGPTIPAPVAAPV------------AATXAPKAAXXXPATTSATLASFEK-WGRGIQKSPISNSLLLRQKAYLALYGTTGTFPVEP 237          
The following BLAST results are available for this feature:
BLAST of Ec-28_003220.2 vs. uniprot
Analysis Date: 2022-09-16 (Diamond blastp: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A6H5KR44_9PHAE1.240e-11886.75Lipoyl-binding domain-containing protein n=1 Tax=E... [more]
D8LTQ3_ECTSI3.860e-102100.00Lipoyl-binding domain-containing protein n=1 Tax=E... [more]
A0A836CM78_9STRA4.540e-4249.32Putative dihydrolipoyllysineresidue acetyltransfer... [more]
A0A485LRT4_9STRA7.280e-3839.59Aste57867_24277 protein n=1 Tax=Aphanomyces stella... [more]
A0A0P1AE60_PLAHL1.020e-3737.14Pyruvate dehydrogenase complex dihydrolipoamide ac... [more]
V9ET80_PHYPR1.610e-3766.67Acetyltransferase component of pyruvate dehydrogen... [more]
A0A8K1FAL3_PYTOL4.460e-3742.22Uncharacterized protein n=1 Tax=Pythium oligandrum... [more]
A0A425C5Z6_9STRA4.330e-3661.67Acetyltransferase component of pyruvate dehydrogen... [more]
A0A0W8DVI9_PHYNI7.890e-3639.02Dihydrolipoyllysine-residue acetyltransferase comp... [more]
A0A1V9YB35_9STRA7.980e-3636.03Dihydrolipoyllysine-residue acetyltransferase comp... [more]

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InterPro
Analysis Name: InterProScan on OGS1.0
Date Performed: 2022-09-29
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D2.40.50.100coord: 41..146
e-value: 6.4E-32
score: 111.4
NoneNo IPR availablePANTHERPTHR43178:SF1DIHYDROLIPOYLLYSINE-RESIDUE ACETYLTRANSFERASE COMPONENT 3 OF PYRUVATE DEHYDROGENASE COMPLEX, MITOCHONDRIALcoord: 22..173
NoneNo IPR availablePANTHERPTHR43178FAMILY NOT NAMEDcoord: 22..173
IPR000089Biotin/lipoyl attachmentPFAMPF00364Biotin_lipoylcoord: 49..120
e-value: 5.0E-13
score: 48.7
IPR000089Biotin/lipoyl attachmentPROSITEPS50968BIOTINYL_LIPOYLcoord: 46..122
score: 21.914
IPR011053Single hybrid motifSUPERFAMILY51230Single hybrid motifcoord: 45..139

Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
chr_28contigchr_28:3055765..3058624 +
Analyses
This polypeptide is derived from or has results from the following analyses
Analysis NameDate Performed
InterProScan on OGS1.02022-09-29
Diamond blastp: OGS1.0 vs UniRef902022-09-16
OGS1.0 of Ectocarpus species7 Ec32 male_plus_femaleSDR2016-10-08
Relationships

This polypeptide derives from the following mRNA feature(s):

Feature NameUnique NameSpeciesTypePosition
Ec-28_003220.2Ec-28_003220.2Ectocarpus species7 Ec32 male_plus_femaleSDRmRNAchr_28 3055437..3059899 +


Sequences
The following sequences are available for this feature:

polypeptide sequence

>Ec-28_003220.2 ID=Ec-28_003220.2|Name=Ec-28_003220.2|organism=Ectocarpus species7 Ec32 male_plus_femaleSDR|type=polypeptide|length=250bp
MIACRRTSTFAARVLATQCQGTRQAASRRSAELVVKRRSLSSLPDHIVVG
MPALSPTMTHGNIGSWGKQEGDEIAAGDVVCEVETDKATVDFDAQDDSFM
AKHLVEAGTQDIAVGTPIFVTVDDADSVAAFKDFEAEAVQGEPEAAAPAA
EAAPNPEEAAPAPAAKKEAPPAPEAAPAPAPAPAPAPSPAPTTAAPVERP
PAPVSSPELVFKRDWGSGVRGSALAHRMSAEQKKYVERFGSTLQQPLDP*
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Annotated Terms
The following terms have been associated with this polypeptide:
Vocabulary: INTERPRO
TermDefinition
IPR000089Biotin_lipoyl
IPR011053Single_hybrid_motif