mRNA_Ecto-sp6_S_contig101.770.1 (mRNA) Ectocarpus species6 EcLAC_371

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_Ecto-sp6_S_contig101.770.1
Unique NamemRNA_Ecto-sp6_S_contig101.770.1
TypemRNA
OrganismEctocarpus species6 EcLAC_371 (Ectocarpus species6 EcLAC_371)
Homology
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BLAST of mRNA_Ecto-sp6_S_contig101.770.1 vs. uniprot
Match: A0A6H5K1Z7_9PHAE (HECT domain-containing protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5K1Z7_9PHAE)

HSP 1 Score: 227 bits (579), Expect = 1.290e-64
Identity = 144/163 (88.34%), Postives = 145/163 (88.96%), Query Frame = 1 
Query:  100 RPAGXXAATATWEKYEWVKVPAPVVQHERLRLLCSVLRLEACSDNSFQRVGTVVSNLSKVPENRMTLLKELVSVAKSLGAVSTHDLQSLEQQLDVAKRDAMARQAAAQXXXXXXXXXXXXXDAPMTSPAVKGKGKAPAHEGSGGGRPGARRNPSCGGQPQLVV 588
            RPAG  AATAT EKYEWVKVPAPVVQHERLRLLCSVLRLEACSDNSFQRVGTVVSNLSKVPENRM LLKELVSVAKSLGAVSTHDLQSLEQQLDVAKRDAMAR AAAQXXXXXXXXXXXXX APMT P VKGKGKAPAHEGSGGGR G R      G P +VV
Sbjct:  296 RPAGAGAATATSEKYEWVKVPAPVVQHERLRLLCSVLRLEACSDNSFQRVGTVVSNLSKVPENRMILLKELVSVAKSLGAVSTHDLQSLEQQLDVAKRDAMAR-AAAQXXXXXXXXXXXXX-APMTLPVVKGKGKAPAHEGSGGGRSGGRA-----GIPPVVV 451
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BLAST of mRNA_Ecto-sp6_S_contig101.770.1 vs. uniprot
Match: D7FHV8_ECTSI (Uncharacterized protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FHV8_ECTSI)

HSP 1 Score: 144 bits (364), Expect = 3.370e-39
Identity = 111/192 (57.81%), Postives = 113/192 (58.85%), Query Frame = 1 
Query:   13 VEEESKMEVDSPXXXXXXXXXXXXXXXXXRPAGXXAATATWEKYEWVKVPAPVVQHERLRLLCSVLRLEACSDNSFQRVGTVVSNLSKVPENRMTLLKELVSVAKSLGAVSTHDLQSLEQQLDVAKRDAMARQAAAQXXXXXXXXXXXXXDAPMTSPAVKGKGKAPAHEGSGGGRPGARRNPSCGGQPQLVV 588
            +EEESKMEVDSPXXXXXXXXXXXXXXXXXRPAG  AATATWEKYEWVKVPAPVVQHERLRLLCSVLRLEACSDNSFQ                                                       QAAAQ             DAPMTSPAVKGKGKAPAHEGSGGGRPG R      G P +VV
Sbjct:   54 IEEESKMEVDSPXXXXXXXXXXXXXXXXXRPAGAGAATATWEKYEWVKVPAPVVQHERLRLLCSVLRLEACSDNSFQ-------------------------------------------------------QAAAQAKQDEGEEAAEEEDAPMTSPAVKGKGKAPAHEGSGGGRPGGRA-----GIPPVVV 185
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BLAST of mRNA_Ecto-sp6_S_contig101.770.1 vs. uniprot
Match: A0A7S1Z2K3_TRICV (Hypothetical protein (Fragment) n=1 Tax=Trieres chinensis TaxID=1514140 RepID=A0A7S1Z2K3_TRICV)

HSP 1 Score: 80.5 bits (197), Expect = 6.950e-14
Identity = 42/81 (51.85%), Postives = 55/81 (67.90%), Query Frame = 1 
Query:  145 EWVKVPAPVVQHERLRLLCSVLRLEACSDNSFQRVGTVVSNLSKVPENRMTLLKELVSVAKSLGAVSTHDLQSLEQQLDVA 387
            E+V VP P+V   RLRLLCS+LRLE+C D SF +V T+   L +V  NR  +L+EL SVA+ LGA +  DL+SL  +L  A
Sbjct:  242 EYVDVPRPLVSPHRLRLLCSLLRLESCRDTSFVKVNTIARRLCRVEANRTCILRELASVAQGLGADAIRDLRSLSIRLSDA 322
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BLAST of mRNA_Ecto-sp6_S_contig101.770.1 vs. uniprot
Match: A0A7S1VJ82_9STRA (Hypothetical protein n=1 Tax=Grammatophora oceanica TaxID=210454 RepID=A0A7S1VJ82_9STRA)

HSP 1 Score: 76.6 bits (187), Expect = 2.960e-12
Identity = 37/79 (46.84%), Postives = 58/79 (73.42%), Query Frame = 1 
Query:  145 EWVKVPAPVVQHERLRLLCSVLRLEACSDNSFQRVGTVVSNLSKVPENRMTLLKELVSVAKSLGAVSTHDLQSLEQQLD 381
            E++ VP  V+  +RLRLLCSVL+LE+C D++F +V T+V  LS+V  NR  +L EL +VA +LGA +  +L++L+ ++D
Sbjct: 2193 EYIGVPRVVISQDRLRLLCSVLKLESCKDSAFTKVNTIVKRLSRVDANRGFILSELGTVAHALGADAIRELRALKIRMD 2271
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BLAST of mRNA_Ecto-sp6_S_contig101.770.1 vs. uniprot
Match: A0A7S2QUU6_9STRA (Hypothetical protein n=2 Tax=Triparma pacifica TaxID=91992 RepID=A0A7S2QUU6_9STRA)

HSP 1 Score: 74.7 bits (182), Expect = 1.250e-11
Identity = 39/74 (52.70%), Postives = 55/74 (74.32%), Query Frame = 1 
Query:  145 EWVKVPAPVVQHERLRLLCSVLRLEACSDNSFQRVGTVVSNLSKVPENRMTLLKELVSVAKSLGAVSTHDLQSL 366
            ++VK P  VV   RLRLLC+VLR E C D+SF +V +++S LSKV  NR+TL++EL SVA++L + +T DL +L
Sbjct:  660 KYVKTPRTVVAARRLRLLCAVLREEVCKDDSFSKVLSIMSALSKVKANRITLVEELGSVAQNLSSDATTDLATL 733
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BLAST of mRNA_Ecto-sp6_S_contig101.770.1 vs. uniprot
Match: B7G082_PHATC (HECT-type E3 ubiquitin transferase (Fragment) n=1 Tax=Phaeodactylum tricornutum (strain CCAP 1055/1) TaxID=556484 RepID=B7G082_PHATC)

HSP 1 Score: 72.4 bits (176), Expect = 8.230e-11
Identity = 36/80 (45.00%), Postives = 51/80 (63.75%), Query Frame = 1 
Query:  148 WVKVPAPVVQHERLRLLCSVLRLEACSDNSFQRVGTVVSNLSKVPENRMTLLKELVSVAKSLGAVSTHDLQSLEQQLDVA 387
            WV+ P   +  ERL+ LCS+LR+E   DN+F +V T+V  L +V  NR  +L EL  VA SL + S HDL++L  ++D A
Sbjct: 3600 WVQAPGIEISPERLKRLCSILRMETFRDNAFSKVNTIVRRLCRVESNRGLVLAELAGVAHSLASDSIHDLRALRIRIDEA 3679
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BLAST of mRNA_Ecto-sp6_S_contig101.770.1 vs. uniprot
Match: A0A7S2W4E8_9STRA (Hypothetical protein n=1 Tax=Eucampia antarctica TaxID=49252 RepID=A0A7S2W4E8_9STRA)

HSP 1 Score: 58.5 bits (140), Expect = 3.580e-6
Identity = 36/77 (46.75%), Postives = 51/77 (66.23%), Query Frame = 2 
Query:  548 AGIPPVVVNLNSSSSELKLLRVLQTLVNLSGKDTEMLEK-------------LLRALDLDGLWDALSSCLTVVSVLE 739
            +G P   V L++SSSELKLLRVLQTL +L G +++  +K             LL+++DL+ LW+ L++CL VVSVLE
Sbjct: 1238 SGTPSSAVTLSTSSSELKLLRVLQTLHSLCGSNSDDNKKSEGGAIVSQELVSLLQSIDLESLWEQLNACLRVVSVLE 1314
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BLAST of mRNA_Ecto-sp6_S_contig101.770.1 vs. uniprot
Match: A0A7S4JHL0_9STRA (Hypothetical protein n=1 Tax=Odontella aurita TaxID=265563 RepID=A0A7S4JHL0_9STRA)

HSP 1 Score: 55.1 bits (131), Expect = 4.740e-5
Identity = 41/98 (41.84%), Postives = 53/98 (54.08%), Query Frame = 2 
Query:  506 RPHMKVAAVAGRGRAGIPP-----VVVNLNSSSSELKLLRVLQTLVNLSGK---------------DTEMLEKLLRALDLDGLWDALSSCLTVVSVLE 739
            R  +  A  +G G+A  P        V L++SSSE+KLLRVLQTL  L G+                T+ L  LL ++DL  LWD L+SCL +VSVLE
Sbjct:  393 REKLTAAEASGDGKARSPEEGRASTAVTLSTSSSEIKLLRVLQTLHVLCGELHDDTAKKHEGNHIFITQELVDLLHSIDLQSLWDQLTSCLRLVSVLE 490
The following BLAST results are available for this feature:
BLAST of mRNA_Ecto-sp6_S_contig101.770.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 8
Match NameE-valueIdentityDescription
A0A6H5K1Z7_9PHAE1.290e-6488.34HECT domain-containing protein n=1 Tax=Ectocarpus ... [more]
D7FHV8_ECTSI3.370e-3957.81Uncharacterized protein n=1 Tax=Ectocarpus silicul... [more]
A0A7S1Z2K3_TRICV6.950e-1451.85Hypothetical protein (Fragment) n=1 Tax=Trieres ch... [more]
A0A7S1VJ82_9STRA2.960e-1246.84Hypothetical protein n=1 Tax=Grammatophora oceanic... [more]
A0A7S2QUU6_9STRA1.250e-1152.70Hypothetical protein n=2 Tax=Triparma pacifica Tax... [more]
B7G082_PHATC8.230e-1145.00HECT-type E3 ubiquitin transferase (Fragment) n=1 ... [more]
A0A7S2W4E8_9STRA3.580e-646.75Hypothetical protein n=1 Tax=Eucampia antarctica T... [more]
A0A7S4JHL0_9STRA4.740e-541.84Hypothetical protein n=1 Tax=Odontella aurita TaxI... [more]
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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
Ecto-sp6_S_contig101contigEcto-sp6_S_contig101:677..3708 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Ectocarpus species6 EcLAC_371 OGS1.02022-07-08
Properties
Property NameValue
Taxonomic scopeEukaryota
Stop1
Start1
Seed ortholog score73.9
Seed ortholog evalue9.3e-11
Seed eggNOG ortholog2850.Phatr36043
Model size740
KEGG koko:K10592,ko:K14772,ko:K17964,ko:K22370
KEGG Pathwayko04120,map04120
Hectar predicted targeting categoryother localisation
Exons5
EggNOG free text desc.ubiquitin protein ligase activity
EggNOG OGsCOG5021@1,KOG0939@2759
Ec32 ortholog descriptionhypothetical protein
Ec32 orthologEc-00_000600.1
EC2.3.2.26
Cds size600
COG Functional cat.O
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko01000,ko03009,ko03029,ko04121
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_Ecto-sp6_S_contig101.770.1prot_Ecto-sp6_S_contig101.770.1Ectocarpus species6 EcLAC_371polypeptideEcto-sp6_S_contig101 1378..3678 -


The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1682348728.1110866-UTR-Ecto-sp6_S_contig101:676..7661682348728.1110866-UTR-Ecto-sp6_S_contig101:676..766Ectocarpus species6 EcLAC_371UTREcto-sp6_S_contig101 677..766 -
1682348728.1225142-UTR-Ecto-sp6_S_contig101:1357..13771682348728.1225142-UTR-Ecto-sp6_S_contig101:1357..1377Ectocarpus species6 EcLAC_371UTREcto-sp6_S_contig101 1358..1377 -
1682348728.1691508-UTR-Ecto-sp6_S_contig101:3678..37081682348728.1691508-UTR-Ecto-sp6_S_contig101:3678..3708Ectocarpus species6 EcLAC_371UTREcto-sp6_S_contig101 3679..3708 -


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1682348728.131147-CDS-Ecto-sp6_S_contig101:1377..15091682348728.131147-CDS-Ecto-sp6_S_contig101:1377..1509Ectocarpus species6 EcLAC_371CDSEcto-sp6_S_contig101 1378..1509 -
1682348728.1399932-CDS-Ecto-sp6_S_contig101:2025..21151682348728.1399932-CDS-Ecto-sp6_S_contig101:2025..2115Ectocarpus species6 EcLAC_371CDSEcto-sp6_S_contig101 2026..2115 -
1682348728.1490886-CDS-Ecto-sp6_S_contig101:2796..29611682348728.1490886-CDS-Ecto-sp6_S_contig101:2796..2961Ectocarpus species6 EcLAC_371CDSEcto-sp6_S_contig101 2797..2961 -
1682348728.1579325-CDS-Ecto-sp6_S_contig101:3465..36781682348728.1579325-CDS-Ecto-sp6_S_contig101:3465..3678Ectocarpus species6 EcLAC_371CDSEcto-sp6_S_contig101 3466..3678 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_Ecto-sp6_S_contig101.770.1

>prot_Ecto-sp6_S_contig101.770.1 ID=prot_Ecto-sp6_S_contig101.770.1|Name=mRNA_Ecto-sp6_S_contig101.770.1|organism=Ectocarpus species6 EcLAC_371|type=polypeptide|length=200bp
MEVDSPPPPAAAATAAATAAAAGRPAGAGAATATWEKYEWVKVPAPVVQH
ERLRLLCSVLRLEACSDNSFQRVGTVVSNLSKVPENRMTLLKELVSVAKS
LGAVSTHDLQSLEQQLDVAKRDAMARQAAAQAKQEEGEEEAEEEDAPMTS
PAVKGKGKAPAHEGSGGGRPGARRNPSCGGQPQLVVERAEAPAGVADPG*
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mRNA from alignment at Ecto-sp6_S_contig101:677..3708-

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_Ecto-sp6_S_contig101.770.1 ID=mRNA_Ecto-sp6_S_contig101.770.1|Name=mRNA_Ecto-sp6_S_contig101.770.1|organism=Ectocarpus species6 EcLAC_371|type=mRNA|length=3032bp|location=Sequence derived from alignment at Ecto-sp6_S_contig101:677..3708- (Ectocarpus species6 EcLAC_371)
GGGTCCCCCGCAGTGGAGGAGGAGTCGAAGATGGAGGTGGATTCCCCTCC
GCCACCTGCGGCGGCGGCTACGGCGGCGGCTACGGCGGCGGCGGCGGGGC
GCCCGGCGGGAGCGGGAGCGGCGACGGCCACTTGGGAGAAGTACGAGTGG
GTGAAGGTGCCGGCCCCCGTGGTTCAGCACGAGAGGCTGCGGCTGCTGTG
CTCGGTGCTACGGCTGGAGGCCTGCAGCGACAATTCCTTCCAGGTGGGTA
GAGGGAGTGTGGTGTGTTGCATGGTGGGCCTTGAGTAGCCCCTCCCCGGC
TACCCCTCGAACCCACGTCCGTCACGACTGTCACCCTGCTGCGCCTGTGT
TCCGCGGGGGAACTCTCCTCTAACGTCCACACGCCTTTCAAGCACGGACG
CCCCGCGCCTCCGCGCGCCCCTTTTGTGACGTTTCTCTGTCCAGGAAGGG
CTGCATGGTCGTGCGCTCAAATGGTTCCTTGTAGGCAAAAGTAGCCAGAA
GCGCGTGTGGTCAGCGCAACACCAACAGACAAGAGGAGTGGGTAGTAATA
CATCTGAGCCCCCCCCCCCCTTCCCCCCCCCCCGCGCGCTCTATCGAAAT
TCCTTGAGTGAGCGGGAACGGTTGTGAGCAACCTTTCAAAGGTCCCGAGC
ATCGCATGCCAATGGTCCAACTACTAGGGCATGCAAAACATATAAGTACG
CCCCCTCCCCGTCCCCCGTCCCCTCGCCCCCTTACAATACCCTTCAGCGA
GTGGGAACTGTGGTGAGCAACCTTTCGAAGGTGCCCGAGAACCGGATGAC
TCTCTTGAAAGAGCTGGTGTCGGTGGCCAAGTCTCTCGGCGCCGTCAGTA
CGCACGACCTGCAGTCGCTGGAGCAACAGCTCGATGTGGCGAAGCGCGAC
GCCATGGCGAGGGTGAGTTTGAGACGTTGGGATGATGGATGGAAGGGCAG
CTTGTAATTCGGTGATTGATAGTCGAGAGAGTGGGATGGGATGAGGTGGC
AGCGGCCAAGGTGTAGGTCTGTGGATAGATTGGACGAGCTCGGCGGTAAT
GATTTGTACCTCAATAATGGGGGGGGGGTTTCACGTTTTGCATGTCTTTG
TTACGACCACGCGTCTTTCCTCGAGAGGACAGAGCGGGATAGGGGGTATT
CACGTTTTGCATGTCTTTGTTACGACCACGCGTCTTCCTCGAGAGGACAG
AGCGGGATGGGGGGGGGGGGGTTACGCAATGTTGTGTTTGCGCGTGCTCG
GTGGCTTGGTTGGGGGTTGAGCCGGGTTACAAACCGCAAACCGGTTTGGG
ATCAGCGCAAACGCCCGCATGAATCCAATGGTGATTTTCCAGCCCCTCGA
CACGCCGCGCACTCGAGAAGGGCCGCCATCGTCGTCGTCGTCGTCGTCCA
GCCTTTTTTTTTTTGGCAAGAAAAGTTGTTGTTCATGACGGGGAAATAAG
CGAGAGTGCGAGAGGGGAAATGTTTGGGCGAGTTGCTATGTTGGGTAGAC
TGAGGGCAATAACTTGCTCACGCTCGCTCGTCATCTCACCCTTGCGCTCT
TTCTGGATCGTCGTCGTCGTTCCAAACTATTGGAACGAAGCAGCAGGCAG
CCGCGCAGGCTAAGCAGGAGGAGGGGGAGGAGGAGGCGGAGGAGGAAGAC
GCACCTATGACGTCGCCGGCAGTGAAGGGCAAGGTGGGTTTGTATTTTGT
TTGTGTGTGTTGACGTAAAGCTTTCAGACTTGATTTTTTGCCCACTGTTT
TGTTGGTGCCTGGCCGCGAACACCAACGGTTGCTCCGCGATGACGAGTTC
GAGATGCTTTTGTTCGCTGTTCTGGCGCAACTGCCGTAGCGTGAAGAACA
CCTGGCTTTCCGAACTACGTTATTCGTGGTGGTTATTATTGTCGTTGTCG
TTGTCGTTGTTGTTGTTGTTGTTGGTATTACCTCACTTGCTTCGATAATT
GTTTCTTGTTAGTCGTTGTTGTTTGATGTCGTTGTCGTTGTCGGCGTCAG
AGTCTCTGGTATAACAGCGCGATGACCGAGGCGGTGGTGTCCTCTGATGC
CCGTTTACGGGGCAAACACACGCTAGTTTTTCGTTTGCAGGGAAAACCCC
GTCGTGTGTGTCCGCCCGCCTCGCTTCTACCCTACCGCGGTCGAACAACG
CGTCACTCCCCTCCCGTCCCTCCCCTCCCTTCGCCTTCCCGCAACTCAGG
GAAAGGCGCCCGCACATGAAGGTAGCGGCGGTGGCAGGCCGGGGGCGCGC
AGGAATCCCTCCTGTGGTGGTCAACCTCAACTCGTCGTCGAGCGAGCTGA
AGCTCCTGCGGGTGTTGCAGACCCTGGTTAACCTTTCGGGGAAGGATACC
GGTGAGGGGTGGTGTGTGATGCCGTGGGGAGTCAGATGGTGGAGATTTGA
TTGTGGCTTGTGACGTTTTTTTGGCCGTTTGCGGGGATGAGGTTTTGGCC
TGTTTGCTGGGATGTTGGGGGCGGGTAGTACGGCGTGGGGATTGATTGGC
CCTTGCCTGTTAGATGGGCCGTGTGCGGGGTTGAGTTTTTTTCCCTGTTT
GCTGAGATGTCGGGGGCGGGTACGATGGCACGGGATTGACCTTTGCTTGT
TAGCTGGGATGTATCTTGGGGGGGTGGGTACACATGGTTGACACCGATGC
GCCAGCCAGTGGAACCGAAGAGATAAAACAAGACGGACAACATAATTTGT
CTGTCAGCAGCAGGTGACCCGTGTGTGTGTACCCTCACGGCCTCCCAGAT
CATGGTAGCCTCTAGAAGCATCTTTGTTTCAGCCGCTTAGCGCTGTCGGT
TCTAATATCAGCGACGCCCGCACCCTCACTCAACCTCACGCACACGACCT
CCCATTTTGTTTCCATGTACGTCTGCCCCCATCCTTCGTCTCACCCGTTT
GTTGGTTCGCTCGGTGGCGGATGGATCGTCGGTCGAGAGCAGAGATGCTT
GAGAAGCTCCTCCGAGCTCTGGACCTGGACGGGCTGTGGGACGCGCTGTC
GTCGTGCCTCACCGTCGTATCCGTGTTGGAGG
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Coding sequence (CDS) from alignment at Ecto-sp6_S_contig101:677..3708-

>mRNA_Ecto-sp6_S_contig101.770.1 ID=mRNA_Ecto-sp6_S_contig101.770.1|Name=mRNA_Ecto-sp6_S_contig101.770.1|organism=Ectocarpus species6 EcLAC_371|type=CDS|length=600bp|location=Sequence derived from alignment at Ecto-sp6_S_contig101:677..3708- (Ectocarpus species6 EcLAC_371)
ATGGAGGTGGATTCCCCTCCGCCACCTGCGGCGGCGGCTACGGCGGCGGC
TACGGCGGCGGCGGCGGGGCGCCCGGCGGGAGCGGGAGCGGCGACGGCCA
CTTGGGAGAAGTACGAGTGGGTGAAGGTGCCGGCCCCCGTGGTTCAGCAC
GAGAGGCTGCGGCTGCTGTGCTCGGTGCTACGGCTGGAGGCCTGCAGCGA
CAATTCCTTCCAGCGAGTGGGAACTGTGGTGAGCAACCTTTCGAAGGTGC
CCGAGAACCGGATGACTCTCTTGAAAGAGCTGGTGTCGGTGGCCAAGTCT
CTCGGCGCCGTCAGTACGCACGACCTGCAGTCGCTGGAGCAACAGCTCGA
TGTGGCGAAGCGCGACGCCATGGCGAGGCAGGCAGCCGCGCAGGCTAAGC
AGGAGGAGGGGGAGGAGGAGGCGGAGGAGGAAGACGCACCTATGACGTCG
CCGGCAGTGAAGGGCAAGGGAAAGGCGCCCGCACATGAAGGTAGCGGCGG
TGGCAGGCCGGGGGCGCGCAGGAATCCCTCCTGTGGTGGTCAACCTCAAC
TCGTCGTCGAGCGAGCTGAAGCTCCTGCGGGTGTTGCAGACCCTGGTTAA
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