mRNA_Ecto-sp6_S_contig10.602.1 (mRNA) Ectocarpus species6 EcLAC_371

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_Ecto-sp6_S_contig10.602.1
Unique NamemRNA_Ecto-sp6_S_contig10.602.1
TypemRNA
OrganismEctocarpus species6 EcLAC_371 (Ectocarpus species6 EcLAC_371)
Homology
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BLAST of mRNA_Ecto-sp6_S_contig10.602.1 vs. uniprot
Match: A0A6H5KTK4_9PHAE (Uncharacterized protein n=2 Tax=Ectocarpus TaxID=2879 RepID=A0A6H5KTK4_9PHAE)

HSP 1 Score: 136 bits (343), Expect = 1.830e-36
Identity = 63/63 (100.00%), Postives = 63/63 (100.00%), Query Frame = 1 
Query:   70 MIIPVRCFTCGKVIGNKWETYLSLLQADYNEGDALDELGLKRYCCRRMVLTHVDLIEKLLNYN 258
            MIIPVRCFTCGKVIGNKWETYLSLLQADYNEGDALDELGLKRYCCRRMVLTHVDLIEKLLNYN
Sbjct:    1 MIIPVRCFTCGKVIGNKWETYLSLLQADYNEGDALDELGLKRYCCRRMVLTHVDLIEKLLNYN 63
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BLAST of mRNA_Ecto-sp6_S_contig10.602.1 vs. uniprot
Match: A0A7S1V7Q3_9STRA (Hypothetical protein n=2 Tax=Bacillariophyta TaxID=2836 RepID=A0A7S1V7Q3_9STRA)

HSP 1 Score: 133 bits (335), Expect = 7.100e-35
Identity = 61/63 (96.83%), Postives = 63/63 (100.00%), Query Frame = 1 
Query:   70 MIIPVRCFTCGKVIGNKWETYLSLLQADYNEGDALDELGLKRYCCRRMVLTHVDLIEKLLNYN 258
            MIIPVRCFTCGKVIGNKWETYLSLLQADY+EGDALDELGLKRYCCRRM+LTHVDLIEKLLNYN
Sbjct:   33 MIIPVRCFTCGKVIGNKWETYLSLLQADYSEGDALDELGLKRYCCRRMLLTHVDLIEKLLNYN 95
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BLAST of mRNA_Ecto-sp6_S_contig10.602.1 vs. uniprot
Match: B8C402_THAPS (DNA directed RNA polymerase II subunit, 8.2kDa, rpb10-like protein (Fragment) n=3 Tax=Bacillariophyta TaxID=2836 RepID=B8C402_THAPS)

HSP 1 Score: 130 bits (326), Expect = 4.400e-34
Identity = 59/63 (93.65%), Postives = 62/63 (98.41%), Query Frame = 1 
Query:   70 MIIPVRCFTCGKVIGNKWETYLSLLQADYNEGDALDELGLKRYCCRRMVLTHVDLIEKLLNYN 258
            MIIPVRCFTCGKVIGNKWETYL LLQADY+EGDALDELGL+RYCCRRM+LTHVDLIEKLLNYN
Sbjct:    1 MIIPVRCFTCGKVIGNKWETYLYLLQADYSEGDALDELGLRRYCCRRMMLTHVDLIEKLLNYN 63
[Close]
BLAST of mRNA_Ecto-sp6_S_contig10.602.1 vs. uniprot
Match: F0YLY9_AURAN (Uncharacterized protein n=1 Tax=Aureococcus anophagefferens TaxID=44056 RepID=F0YLY9_AURAN)

HSP 1 Score: 130 bits (326), Expect = 6.330e-34
Identity = 59/63 (93.65%), Postives = 62/63 (98.41%), Query Frame = 1 
Query:   70 MIIPVRCFTCGKVIGNKWETYLSLLQADYNEGDALDELGLKRYCCRRMVLTHVDLIEKLLNYN 258
            MIIPVRCFTCGKVIGNKWE+YL LL+ADY+EGDALDELGLKRYCCRRMVLTHVDLIEKLLNYN
Sbjct:    1 MIIPVRCFTCGKVIGNKWESYLELLKADYSEGDALDELGLKRYCCRRMVLTHVDLIEKLLNYN 63
[Close]
BLAST of mRNA_Ecto-sp6_S_contig10.602.1 vs. uniprot
Match: A0A1E7EXD3_9STRA (DNA-directed RNA polymerases I, II, and III subunit rpabc5 n=1 Tax=Fragilariopsis cylindrus CCMP1102 TaxID=635003 RepID=A0A1E7EXD3_9STRA)

HSP 1 Score: 129 bits (325), Expect = 7.700e-34
Identity = 59/63 (93.65%), Postives = 62/63 (98.41%), Query Frame = 1 
Query:   70 MIIPVRCFTCGKVIGNKWETYLSLLQADYNEGDALDELGLKRYCCRRMVLTHVDLIEKLLNYN 258
            MIIPVRCFTCGKVI NKWETYLSLLQAD++EGDALDELGLKRYCCRRM+LTHVDLIEKLLNYN
Sbjct:    1 MIIPVRCFTCGKVIANKWETYLSLLQADFSEGDALDELGLKRYCCRRMMLTHVDLIEKLLNYN 63
[Close]
BLAST of mRNA_Ecto-sp6_S_contig10.602.1 vs. uniprot
Match: A0A7R9WWK7_9STRA (Hypothetical protein n=1 Tax=Craspedostauros australis TaxID=1486917 RepID=A0A7R9WWK7_9STRA)

HSP 1 Score: 132 bits (331), Expect = 8.900e-34
Identity = 60/63 (95.24%), Postives = 63/63 (100.00%), Query Frame = 1 
Query:   70 MIIPVRCFTCGKVIGNKWETYLSLLQADYNEGDALDELGLKRYCCRRMVLTHVDLIEKLLNYN 258
            MIIPVRCFTCGKVIGNKWETYLSLLQAD++EGDALDELGLKRYCCRRM+LTHVDLIEKLLNYN
Sbjct:   74 MIIPVRCFTCGKVIGNKWETYLSLLQADFSEGDALDELGLKRYCCRRMMLTHVDLIEKLLNYN 136
[Close]
BLAST of mRNA_Ecto-sp6_S_contig10.602.1 vs. uniprot
Match: A0A836CHW2_9STRA (DNA-directed RNA polymerase I, II, and III subunit RPABC5 n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A836CHW2_9STRA)

HSP 1 Score: 129 bits (323), Expect = 1.600e-33
Identity = 59/63 (93.65%), Postives = 61/63 (96.83%), Query Frame = 1 
Query:   70 MIIPVRCFTCGKVIGNKWETYLSLLQADYNEGDALDELGLKRYCCRRMVLTHVDLIEKLLNYN 258
            MIIPVRCFTCGKVIGNKWETYL LLQ+D +EGDALDELGLKRYCCRRMVLTHVDLIEKLLNYN
Sbjct:    1 MIIPVRCFTCGKVIGNKWETYLGLLQSDISEGDALDELGLKRYCCRRMVLTHVDLIEKLLNYN 63
[Close]
BLAST of mRNA_Ecto-sp6_S_contig10.602.1 vs. uniprot
Match: A0A7S4EET2_9STRA (Hypothetical protein n=1 Tax=Pseudo-nitzschia australis TaxID=44445 RepID=A0A7S4EET2_9STRA)

HSP 1 Score: 129 bits (325), Expect = 2.030e-33
Identity = 59/63 (93.65%), Postives = 62/63 (98.41%), Query Frame = 1 
Query:   70 MIIPVRCFTCGKVIGNKWETYLSLLQADYNEGDALDELGLKRYCCRRMVLTHVDLIEKLLNYN 258
            MIIPVRCFTCGKVI NKWETYLSLLQAD++EGDALDELGLKRYCCRRM+LTHVDLIEKLLNYN
Sbjct:   34 MIIPVRCFTCGKVIANKWETYLSLLQADFSEGDALDELGLKRYCCRRMMLTHVDLIEKLLNYN 96
[Close]
BLAST of mRNA_Ecto-sp6_S_contig10.602.1 vs. uniprot
Match: A0A8J2SAT8_9STRA (Hypothetical protein n=1 Tax=Pelagomonas calceolata TaxID=35677 RepID=A0A8J2SAT8_9STRA)

HSP 1 Score: 128 bits (322), Expect = 2.380e-33
Identity = 57/63 (90.48%), Postives = 62/63 (98.41%), Query Frame = 1 
Query:   70 MIIPVRCFTCGKVIGNKWETYLSLLQADYNEGDALDELGLKRYCCRRMVLTHVDLIEKLLNYN 258
            MIIPVRCFTCGKV+GNKW++YL LL+ADY+EGDALDELGLKRYCCRRMVLTHVDLIEKLLNYN
Sbjct:    1 MIIPVRCFTCGKVVGNKWDSYLELLKADYSEGDALDELGLKRYCCRRMVLTHVDLIEKLLNYN 63
[Close]
BLAST of mRNA_Ecto-sp6_S_contig10.602.1 vs. uniprot
Match: A0A091D7D0_FUKDA (DNA-directed RNA polymerases I, II, and III subunit RPABC5 n=1 Tax=Fukomys damarensis TaxID=885580 RepID=A0A091D7D0_FUKDA)

HSP 1 Score: 127 bits (320), Expect = 6.240e-33
Identity = 57/72 (79.17%), Postives = 63/72 (87.50%), Query Frame = 1 
Query:   40 SPHTCTASSTMIIPVRCFTCGKVIGNKWETYLSLLQADYNEGDALDELGLKRYCCRRMVLTHVDLIEKLLNY 255
            +P  C A +TMIIPVRCFTCGK++GNKWE YL LLQA+Y EGDALD LGLKRYCCRRM+L HVDLIEKLLNY
Sbjct:   10 TPQGCRAVATMIIPVRCFTCGKIVGNKWEAYLGLLQAEYTEGDALDALGLKRYCCRRMLLAHVDLIEKLLNY 81
The following BLAST results are available for this feature:
BLAST of mRNA_Ecto-sp6_S_contig10.602.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A6H5KTK4_9PHAE1.830e-36100.00Uncharacterized protein n=2 Tax=Ectocarpus TaxID=2... [more]
A0A7S1V7Q3_9STRA7.100e-3596.83Hypothetical protein n=2 Tax=Bacillariophyta TaxID... [more]
B8C402_THAPS4.400e-3493.65DNA directed RNA polymerase II subunit, 8.2kDa, rp... [more]
F0YLY9_AURAN6.330e-3493.65Uncharacterized protein n=1 Tax=Aureococcus anopha... [more]
A0A1E7EXD3_9STRA7.700e-3493.65DNA-directed RNA polymerases I, II, and III subuni... [more]
A0A7R9WWK7_9STRA8.900e-3495.24Hypothetical protein n=1 Tax=Craspedostauros austr... [more]
A0A836CHW2_9STRA1.600e-3393.65DNA-directed RNA polymerase I, II, and III subunit... [more]
A0A7S4EET2_9STRA2.030e-3393.65Hypothetical protein n=1 Tax=Pseudo-nitzschia aust... [more]
A0A8J2SAT8_9STRA2.380e-3390.48Hypothetical protein n=1 Tax=Pelagomonas calceolat... [more]
A0A091D7D0_FUKDA6.240e-3379.17DNA-directed RNA polymerases I, II, and III subuni... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
Ecto-sp6_S_contig10contigEcto-sp6_S_contig10:1912074..1914228 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Ectocarpus species6 EcLAC_371 OGS1.02022-07-08
Properties
Property NameValue
Taxonomic scopeEukaryota
Stop1
Start1
Seed ortholog score136.0
Seed ortholog evalue8.5e-30
Seed eggNOG ortholog2850.Phatr10821
Preferred namePOLR2L
Model size1156
KEGG rclassRC02795
KEGG koko:K02912,ko:K03007,ko:K11313,ko:K15271
KEGG ReactionR00435,R00441,R00442,R00443
KEGG Pathwayko00230,ko00240,ko01100,ko03010,ko03020,ko04623,ko05016,ko05169,ko05202,map00230,map00240,map01100,map03010,map03020,map04623,map05016,map05169,map05202
KEGG ModuleM00177,M00179,M00180,M00181,M00182
Hectar predicted targeting categoryother localisation
GOsGO:0000375,GO:0000377,GO:0000398,GO:0000418,GO:0000419,GO:0000428,GO:0003674,GO:0003824,GO:0003899,GO:0005488,GO:0005575,GO:0005622,GO:0005623,GO:0005634,GO:0005654,GO:0005665,GO:0005666,GO:0005730,GO:0005736,GO:0005737,GO:0005829,GO:0006139,GO:0006259,GO:0006281,GO:0006283,GO:0006289,GO:0006351,GO:0006352,GO:0006353,GO:0006354,GO:0006355,GO:0006356,GO:0006360,GO:0006361,GO:0006363,GO:0006366,GO:0006367,GO:0006368,GO:0006370,GO:0006383,GO:0006396,GO:0006397,GO:0006399,GO:0006725,GO:0006807,GO:0006950,GO:0006974,GO:0007154,GO:0007165,GO:0007166,GO:0007167,GO:0007169,GO:0008150,GO:0008152,GO:0008270,GO:0008380,GO:0008543,GO:0009058,GO:0009059,GO:0009301,GO:0009304,GO:0009452,GO:0009719,GO:0009889,GO:0009893,GO:0009987,GO:0010033,GO:0010467,GO:0010468,GO:0010556,GO:0010604,GO:0010628,GO:0016070,GO:0016071,GO:0016073,GO:0016591,GO:0016740,GO:0016772,GO:0016779,GO:0018130,GO:0019219,GO:0019222,GO:0019438,GO:0023052,GO:0030880,GO:0031323,GO:0031326,GO:0031974,GO:0031981,GO:0032774,GO:0032991,GO:0033554,GO:0034062,GO:0034641,GO:0034645,GO:0034654,GO:0034660,GO:0036260,GO:0040029,GO:0042221,GO:0042795,GO:0042797,GO:0043167,GO:0043169,GO:0043170,GO:0043226,GO:0043227,GO:0043228,GO:0043229,GO:0043231,GO:0043232,GO:0043233,GO:0044237,GO:0044238,GO:0044249,GO:0044260,GO:0044271,GO:0044344,GO:0044422,GO:0044424,GO:0044428,GO:0044444,GO:0044446,GO:0044451,GO:0044452,GO:0044464,GO:0045815,GO:0046483,GO:0046872,GO:0046914,GO:0048518,GO:0050789,GO:0050794,GO:0050896,GO:0051171,GO:0051252,GO:0051716,GO:0055029,GO:0060147,GO:0060255,GO:0060964,GO:0060966,GO:0060968,GO:0061695,GO:0065007,GO:0070013,GO:0070848,GO:0070887,GO:0071310,GO:0071363,GO:0071495,GO:0071704,GO:0071774,GO:0080090,GO:0090304,GO:0097659,GO:0097747,GO:0098781,GO:0140098,GO:1901360,GO:1901362,GO:1901576,GO:1902494,GO:1903506,GO:1990234,GO:2000112,GO:2001141
Exons3
EggNOG free text desc.RNA polymerases N / 8 kDa subunit
EggNOG OGs2XH5G@2836,COG1644@1,KOG3497@2759
Ec32 ortholog descriptionDNA-directed RNA polymerase, subunit N/Rpb10
Ec32 orthologEc-15_003650.1
EC3.6.4.12
Cds size258
COG Functional cat.K
Best tax levelBacillariophyta
Best eggNOG OGNA|NA|NA
BRITEbr01610,br01611,ko00000,ko00001,ko00002,ko01000,ko03011,ko03021,ko03036,ko03400
Relationships

The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1682348712.3269541-UTR-Ecto-sp6_S_contig10:1912073..19128221682348712.3269541-UTR-Ecto-sp6_S_contig10:1912073..1912822Ectocarpus species6 EcLAC_371UTREcto-sp6_S_contig10 1912074..1912822 -
1682348712.3397179-UTR-Ecto-sp6_S_contig10:1913073..19131531682348712.3397179-UTR-Ecto-sp6_S_contig10:1913073..1913153Ectocarpus species6 EcLAC_371UTREcto-sp6_S_contig10 1913074..1913153 -
1682348712.3654244-UTR-Ecto-sp6_S_contig10:1914159..19142281682348712.3654244-UTR-Ecto-sp6_S_contig10:1914159..1914228Ectocarpus species6 EcLAC_371UTREcto-sp6_S_contig10 1914160..1914228 -


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1682348712.347698-CDS-Ecto-sp6_S_contig10:1913153..19132211682348712.347698-CDS-Ecto-sp6_S_contig10:1913153..1913221Ectocarpus species6 EcLAC_371CDSEcto-sp6_S_contig10 1913154..1913221 -
1682348712.3558223-CDS-Ecto-sp6_S_contig10:1913969..19141591682348712.3558223-CDS-Ecto-sp6_S_contig10:1913969..1914159Ectocarpus species6 EcLAC_371CDSEcto-sp6_S_contig10 1913970..1914159 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_Ecto-sp6_S_contig10.602.1prot_Ecto-sp6_S_contig10.602.1Ectocarpus species6 EcLAC_371polypeptideEcto-sp6_S_contig10 1913154..1914159 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_Ecto-sp6_S_contig10.602.1

>prot_Ecto-sp6_S_contig10.602.1 ID=prot_Ecto-sp6_S_contig10.602.1|Name=mRNA_Ecto-sp6_S_contig10.602.1|organism=Ectocarpus species6 EcLAC_371|type=polypeptide|length=86bp
MIIPVRCFTCGKVIGNKWETYLSLLQADYNEGDALDELGLKRYCCRRMVL
THVDLIEKLLNYNWGVVPYVKWLRSRAPDGQIRNS*
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mRNA from alignment at Ecto-sp6_S_contig10:1912074..1914228-

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_Ecto-sp6_S_contig10.602.1 ID=mRNA_Ecto-sp6_S_contig10.602.1|Name=mRNA_Ecto-sp6_S_contig10.602.1|organism=Ectocarpus species6 EcLAC_371|type=mRNA|length=2155bp|location=Sequence derived from alignment at Ecto-sp6_S_contig10:1912074..1914228- (Ectocarpus species6 EcLAC_371)
GTGACACACACACGTTGCTGCTTTGCTGTTGCCTCGGGCTCACCACACAC
GTGCACCGCCAGTAGCACGATGATCATCCCGGTGCGATGCTTCACATGCG
GTAAGGTAATCGGAAACAAGTGGGAGACGTACTTGAGCCTGCTGCAGGCG
GACTACAACGAGGGAGATGCGCTGGACGAGCTCGGGCTCAAGAGATACTG
CTGCCGACGAATGGTGCTCACTCACGTAGATCTCATCGAGAAGCTGCTCA
ACTACAACTGTGAGTGCCCCTAGGGACAAATCTTCAAACAGTAAGCGCAG
GCGGGAAGTAGATACGCTGGGTGTCTACGCACAAGAACGTGGCGTTTAAA
ATGTGCTGCCCGTTTGCATATCCACCTATGTCCCCACCGCTACCGCTGCT
GTTAGACGACCTTTACACCTACACCCATCCTTCCGGCCTTGGGAGAACAG
GAACAGTATGGCTGCCTAGCTGGTAGGTACAAGCATGCTTCGTTGTGCGC
CGTACGGAGAGCGTATGGGCACATGGAAGAGGTTCCCGACGCCGAGTCCA
GTCTAGCACTTGTGTTGGGTGCGCCGCTCTTTCTGTAGACCCTCACGGAG
TTGATGTTGGACCGCCCGCCGAGTGCAGCTTATGGCGCGCGACCCACAAC
ACCAGAGCATCAGTAATACATGTAAATGCGTCGCTTAGGTGTTGGTGATC
ATTGGGCTGAGGTCAGGGCTGCAGCTAGGTTTCTTTGGATCCTGAATTCC
CAGCACCATACAAATGTTTTCACCCAGATTAAGGGTACCGTGGGTAACAT
GACACAAGATACCACACCCCCTGCACCGATTTTCAAAGCTCGACTACTGC
TTCACTGCTGTAGTCAGGCAAATCACGTACACGTAGAGGATGAAAAAATT
CAAAACGACGCATTTACGAACAGCTCTCGACGAGGTGGTTTCATCGATGC
CGTTTTCGGCACCGGCGCTCTCCCTGCTGCGGAATCGCGCTATGACATTC
GATCCAGGGGGTGTGGTACCTTACGTCAAATGGTTGCGGAGCAGAGCACC
TGACGGACAGATACGCAATTCGTGAGGGTATTCTCTGCACAGGCGAACAG
CTCGTCGAATGCTGCGCGCGCCATGCTTGTCGGGCAAAGAATGCCTCTAA
CAAAGATACAATCACCATGTTGCCGAATTTTTTGCCACAGGTGTTATCGT
TGTTAAGCCTTTGCACCTATGCATAGCTCCTACCCATACAGCAGCAGTGT
AGCATCAGATGCTGTGACGATTGATGCTCCCGCTGGTGGAGTTTACATGA
TGCGCACCCACACATCCACAAAGCCCTGTTCAACAGGGAAATGAGAAGTA
ATTCCCTTACTCCCGTGCCGTTTTCTTCTTCCGCCCTTGCCCTCCTCGAC
AATCAGCACTGGAGCGGAGAACAGGGCCAGAGACAGACTAGGACGCCAGC
TTTTTTCGGGCTGAGAAGAGAGGATGGAGTGGAACAGTAGACCGATCGAA
GCTGAAGGGGGGGGGGGAGTTTCATTGATGGTTGTGATGGGCCTCTACTT
TGCTTCACTTCCCTAGGACGGTCTGTTGGCGTGTGGTTGAAGTGACATGC
ACGTTGTCGGGAGCTTCAGTCATCATGTTGCTTTGGTTCGCTGCGAGGCG
TCCAAGCTCATCTTGGAGTCGATAACTCCGTCGTTAAGAACTTGCGACGC
CGCTAGCGGTGGATGCGTCGAACACAGTAGGGGGTCTACTCATGCCTCTT
CGCACGTCCGAACTTCTGTGACCTACCTGGTGCTGTGCTACAATAGTCTG
GAGAGTGTGGTCCACCCCACGGACGAACCCATGTTGGGGGATGATGTGTG
ATTCGCTGTAAGATATATTTGTGCGTTTTCGGTGCATGTTTCTCGTGCGG
TTTTCTTGCCTGTTTTTTTTTGTGTGTGTCTAGCCAATGCATTTTTTTTT
TGTGCGGTCCTGATTCAACTTGGGGCGCGGGGCGCGCCTTCAACGCCGGG
AATGCATAGGTGCTACACAATTGGAAGAGATGGCTGTTGGCTTCATTTTG
TTTGTCTCTGTTTTCTGTTTGTCAATGTTTTGTTTTGTGCTGAAAAACTA
AATGGAGACCTCAACTATTTGTGAAGACAATGACAACAACACCCTTTCGA
GCAGG
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Coding sequence (CDS) from alignment at Ecto-sp6_S_contig10:1912074..1914228-

>mRNA_Ecto-sp6_S_contig10.602.1 ID=mRNA_Ecto-sp6_S_contig10.602.1|Name=mRNA_Ecto-sp6_S_contig10.602.1|organism=Ectocarpus species6 EcLAC_371|type=CDS|length=258bp|location=Sequence derived from alignment at Ecto-sp6_S_contig10:1912074..1914228- (Ectocarpus species6 EcLAC_371)
ATGATCATCCCGGTGCGATGCTTCACATGCGGTAAGGTAATCGGAAACAA
GTGGGAGACGTACTTGAGCCTGCTGCAGGCGGACTACAACGAGGGAGATG
CGCTGGACGAGCTCGGGCTCAAGAGATACTGCTGCCGACGAATGGTGCTC
ACTCACGTAGATCTCATCGAGAAGCTGCTCAACTACAACTGGGGTGTGGT
ACCTTACGTCAAATGGTTGCGGAGCAGAGCACCTGACGGACAGATACGCA
ATTCGTGA
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