mRNA_Ecto-sp13_S_contig16676.4780.1 (mRNA) Ectocarpus species13 EcNAP12_S_4_19m

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_Ecto-sp13_S_contig16676.4780.1
Unique NamemRNA_Ecto-sp13_S_contig16676.4780.1
TypemRNA
OrganismEctocarpus species13 EcNAP12_S_4_19m (Ectocarpus species13 EcNAP12_S_4_19m)
Homology
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BLAST of mRNA_Ecto-sp13_S_contig16676.4780.1 vs. uniprot
Match: D8LFY0_ECTSI (Threonine--tRNA ligase n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LFY0_ECTSI)

HSP 1 Score: 193 bits (490), Expect = 5.760e-56
Identity = 101/104 (97.12%), Postives = 103/104 (99.04%), Query Frame = 1 
Query:    1 EEGRGNIGGDFTLQANPKFLKERSAKFDAIAARQKERLAQKPKLEITVVLPDGTEKKGVSYETTPMDIAKGISKNLAKEVVVAEVRYSSRIEDDEQNVSSADGI 312
            EEGRGNIGGDFTLQ NPKFLKERSAKFDAIAARQKERLAQKPKLEI+VVLPDGTEKKGVSYETTPMDIAKGISKNLAKEVVVAEVRYSSRIEDDEQNVSSADG+
Sbjct:   69 EEGRGNIGGDFTLQGNPKFLKERSAKFDAIAARQKERLAQKPKLEISVVLPDGTEKKGVSYETTPMDIAKGISKNLAKEVVVAEVRYSSRIEDDEQNVSSADGM 172
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BLAST of mRNA_Ecto-sp13_S_contig16676.4780.1 vs. uniprot
Match: A0A835YMV8_9STRA (Threonine--tRNA ligase n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835YMV8_9STRA)

HSP 1 Score: 123 bits (308), Expect = 1.800e-30
Identity = 60/104 (57.69%), Postives = 83/104 (79.81%), Query Frame = 1 
Query:    1 EEGRGNIGGDFTLQANPKFLKERSAKFDAIAARQKERLAQKPKLEITVVLPDGTEKKGVSYETTPMDIAKGISKNLAKEVVVAEVRYSSRIEDDEQNVSSADGI 312
            E  +G IG  FT+  +P FLK+R A F+A AA+Q+ER A KP++ IT+ LPDG+ ++G+S++TTPMDIA  ISK LAK+++V+EV YSS+IEDDE NV+SADG+
Sbjct:   55 ESAQGRIGAPFTVTKDPAFLKDRMAVFEAAAAKQQERHAAKPRVPITITLPDGSVREGISWQTTPMDIATAISKQLAKQIIVSEVTYSSKIEDDEANVASADGL 158
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BLAST of mRNA_Ecto-sp13_S_contig16676.4780.1 vs. uniprot
Match: H6WB86_VAULI (Threonine--tRNA ligase n=1 Tax=Vaucheria litorea TaxID=109269 RepID=H6WB86_VAULI)

HSP 1 Score: 106 bits (265), Expect = 1.160e-24
Identity = 54/100 (54.00%), Postives = 72/100 (72.00%), Query Frame = 1 
Query:   13 GNIGGDFTLQANPKFLKERSAKFDAIAARQKERLAQKPKLEITVVLPDGTEKKGVSYETTPMDIAKGISKNLAKEVVVAEVRYSSRIEDDEQNVSSADGI 312
            G IGG F L++NP F++ER   ++    +Q  R   KPK+EIT+ LPDG+ K G S+ TTPMDIA+ ISK +AK+V+VAEV YS +IEDDE +V  ADG+
Sbjct:   59 GRIGGSFNLESNPHFIEERKEVYEQAMKKQISRQQTKPKVEITITLPDGSTKVGTSWLTTPMDIAQSISKQMAKKVIVAEVIYSRKIEDDEVDVVGADGL 158
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BLAST of mRNA_Ecto-sp13_S_contig16676.4780.1 vs. uniprot
Match: A0A067CSB6_SAPPC (Threonine--tRNA ligase n=3 Tax=Saprolegniaceae TaxID=4764 RepID=A0A067CSB6_SAPPC)

HSP 1 Score: 96.3 bits (238), Expect = 5.110e-21
Identity = 52/96 (54.17%), Postives = 70/96 (72.92%), Query Frame = 1 
Query:   19 IGGDFTLQANPKFLKERSAKFDAIAARQKERLAQKPKLEITVVLPDGTEKKGVSYETTPMDIAKGISKNLAKEVVVAEVRYSSRIEDDEQNVSSAD 306
            IGG FTL+ NP F++ R A +D + A QKE LA +P+  I + LPDGT K+GVS+ETTP+DIAKGIS+ LA  VVVA+V+Y+    +D   V++AD
Sbjct:   42 IGGAFTLEPNPAFIQRRIAVYDRVMAAQKELLAAQPRKPIKITLPDGTVKEGVSWETTPLDIAKGISQGLADSVVVAKVKYAGS-PNDPLKVTAAD 136
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BLAST of mRNA_Ecto-sp13_S_contig16676.4780.1 vs. uniprot
Match: A0A7J6PXH8_PEROL (TGS domain-containing protein n=1 Tax=Perkinsus olseni TaxID=32597 RepID=A0A7J6PXH8_PEROL)

HSP 1 Score: 87.8 bits (216), Expect = 5.160e-19
Identity = 51/100 (51.00%), Postives = 66/100 (66.00%), Query Frame = 1 
Query:   13 GNIGGDFTLQAN--PKFLKERSAKFDAIAARQKERLAQKPKLEITVVLPDGTEKKGVSYETTPMDIAKGISKNLAKEVVVAEVRYSSRIEDDEQNVSSAD 306
            G   GDF +  N    FLKERS  FD + A Q+ERLA  PK  IT+ LPDGT+K+G  +ETTP+DIA GISK LA  VV A+V Y+  ++  +Q V++ D
Sbjct:   22 GKFEGDFKIYPNGTAPFLKERSELFDKLYAAQEERLAALPKDPITIELPDGTKKEGHKFETTPLDIALGISKGLANSVVAAKVLYTEPVDSMDQVVAADD 121
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BLAST of mRNA_Ecto-sp13_S_contig16676.4780.1 vs. uniprot
Match: A0A1V9ZZW8_9STRA (Threonine--tRNA ligase n=1 Tax=Thraustotheca clavata TaxID=74557 RepID=A0A1V9ZZW8_9STRA)

HSP 1 Score: 90.1 bits (222), Expect = 7.410e-19
Identity = 44/87 (50.57%), Postives = 63/87 (72.41%), Query Frame = 1 
Query:   19 IGGDFTLQANPKFLKERSAKFDAIAARQKERLAQKPKLEITVVLPDGTEKKGVSYETTPMDIAKGISKNLAKEVVVAEVRYSSRIED 279
            IGG F+++ NP FL+ R A +D + A+QK+ LA +P+  I + LPDG  K+G+S+ETTP+DIAK IS+ LA  VVVA+V+Y+    D
Sbjct:   37 IGGPFSIEPNPAFLQRRIAVYDRVMAQQKQVLASQPRKPIKITLPDGNVKEGISWETTPLDIAKSISQGLADSVVVAKVKYAGNPHD 123
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BLAST of mRNA_Ecto-sp13_S_contig16676.4780.1 vs. uniprot
Match: A0A3R7WMR9_9STRA (Threonine--tRNA ligase n=9 Tax=Aphanomyces TaxID=100860 RepID=A0A3R7WMR9_9STRA)

HSP 1 Score: 89.0 bits (219), Expect = 1.900e-18
Identity = 44/87 (50.57%), Postives = 63/87 (72.41%), Query Frame = 1 
Query:   19 IGGDFTLQANPKFLKERSAKFDAIAARQKERLAQKPKLEITVVLPDGTEKKGVSYETTPMDIAKGISKNLAKEVVVAEVRYSSRIED 279
            IG  FT++ NP FL+ R A +D + A+QKE +A +P+  I + LPDG  K+G+S+ETTP+DIAK IS+ LA+ VVVA+V+Y  +  D
Sbjct:  152 IGLPFTVEPNPAFLQRRIAVYDRVMAKQKEEIAAQPRKPIKITLPDGNVKEGISWETTPLDIAKAISQGLAETVVVAKVKYVGKPHD 238
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BLAST of mRNA_Ecto-sp13_S_contig16676.4780.1 vs. uniprot
Match: A0A7J6MZ35_PERCH (Threonine--tRNA ligase n=1 Tax=Perkinsus chesapeaki TaxID=330153 RepID=A0A7J6MZ35_PERCH)

HSP 1 Score: 87.8 bits (216), Expect = 4.800e-18
Identity = 52/100 (52.00%), Postives = 66/100 (66.00%), Query Frame = 1 
Query:   13 GNIGGDFTLQAN--PKFLKERSAKFDAIAARQKERLAQKPKLEITVVLPDGTEKKGVSYETTPMDIAKGISKNLAKEVVVAEVRYSSRIEDDEQNVSSAD 306
            G   GDF +  N    FLKERS  FD + A Q+ERLA  PK  IT+ LPDGT K+G  +ETTP+DIA GISK LA  V+VA+V Y+  +E  +Q V++ D
Sbjct:   22 GKFEGDFKIYPNGTAPFLKERSELFDKLYAAQEERLAALPKDPITIELPDGTIKEGHKFETTPLDIALGISKGLANSVMVAKVLYTEPVESMDQVVAADD 121
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BLAST of mRNA_Ecto-sp13_S_contig16676.4780.1 vs. uniprot
Match: A0A485LGS8_9STRA (Threonine--tRNA ligase n=2 Tax=Aphanomyces TaxID=100860 RepID=A0A485LGS8_9STRA)

HSP 1 Score: 87.0 bits (214), Expect = 8.900e-18
Identity = 44/87 (50.57%), Postives = 61/87 (70.11%), Query Frame = 1 
Query:   19 IGGDFTLQANPKFLKERSAKFDAIAARQKERLAQKPKLEITVVLPDGTEKKGVSYETTPMDIAKGISKNLAKEVVVAEVRYSSRIED 279
            IG  FT++ NP FL+ R A +D I  +QK  +A +P+  I + LPDG  K+GVS+ETTP+DIAK IS+ LA+ +VVA+V+Y  R  D
Sbjct:   40 IGLPFTVEPNPAFLQRRIAVYDRIMEKQKAEIAAQPRTPIKITLPDGNVKEGVSWETTPLDIAKSISQGLAESIVVAKVKYVGRPHD 126
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BLAST of mRNA_Ecto-sp13_S_contig16676.4780.1 vs. uniprot
Match: A0A8J8P0U6_HALGN (Threonine--tRNA ligase n=1 Tax=Halteria grandinella TaxID=5974 RepID=A0A8J8P0U6_HALGN)

HSP 1 Score: 86.3 bits (212), Expect = 1.670e-17
Identity = 40/85 (47.06%), Postives = 64/85 (75.29%), Query Frame = 1 
Query:   19 IGGDFTLQANPKFLKERSAKFDAIAARQKERLAQKPKLEITVVLPDGTEKKGVSYETTPMDIAKGISKNLAKEVVVAEVRYSSRI 273
            IGGD  +  NP F+ ER A F+ + A Q+ +  + P+ +I ++LPDG EK+G S+ETTP+DIAKGIS +LA+++VV++V++ +R+
Sbjct:   38 IGGDIKMAKNPAFIPERKAIFEELYAVQQAKYKEMPRQKIRILLPDGKEKEGTSFETTPLDIAKGISNSLAEKLVVSKVKFLNRV 122
The following BLAST results are available for this feature:
BLAST of mRNA_Ecto-sp13_S_contig16676.4780.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
D8LFY0_ECTSI5.760e-5697.12Threonine--tRNA ligase n=1 Tax=Ectocarpus siliculo... [more]
A0A835YMV8_9STRA1.800e-3057.69Threonine--tRNA ligase n=1 Tax=Tribonema minus Tax... [more]
H6WB86_VAULI1.160e-2454.00Threonine--tRNA ligase n=1 Tax=Vaucheria litorea T... [more]
A0A067CSB6_SAPPC5.110e-2154.17Threonine--tRNA ligase n=3 Tax=Saprolegniaceae Tax... [more]
A0A7J6PXH8_PEROL5.160e-1951.00TGS domain-containing protein n=1 Tax=Perkinsus ol... [more]
A0A1V9ZZW8_9STRA7.410e-1950.57Threonine--tRNA ligase n=1 Tax=Thraustotheca clava... [more]
A0A3R7WMR9_9STRA1.900e-1850.57Threonine--tRNA ligase n=9 Tax=Aphanomyces TaxID=1... [more]
A0A7J6MZ35_PERCH4.800e-1852.00Threonine--tRNA ligase n=1 Tax=Perkinsus chesapeak... [more]
A0A485LGS8_9STRA8.900e-1850.57Threonine--tRNA ligase n=2 Tax=Aphanomyces TaxID=1... [more]
A0A8J8P0U6_HALGN1.670e-1747.06Threonine--tRNA ligase n=1 Tax=Halteria grandinell... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
Ecto-sp13_S_contig16676contigEcto-sp13_S_contig16676:31..2771 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Ectocarpus species13 EcNAP12_S_4_19m OGS1.02022-07-08
Properties
Property NameValue
Taxonomic scopeEukaryota
Stop0
Start0
Seed ortholog score195.7
Seed ortholog evalue1.1e-47
Seed eggNOG ortholog2880.D8LFY0
Model size314
KEGG rclassRC00055,RC00523
KEGG koko:K01868
KEGG ReactionR03663
KEGG Pathwayko00970,map00970
KEGG ModuleM00359,M00360
Hectar predicted targeting categoryother localisation
Exons4
EggNOG free text desc.threonyl-tRNA aminoacylation
EggNOG OGsCOG0441@1,KOG1637@2759
EC6.1.1.3
Cds size312
COG Functional cat.J
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko00002,ko01000,ko01007,ko03016
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1681462874.1648595-CDS-Ecto-sp13_S_contig16676:32..871681462874.1648595-CDS-Ecto-sp13_S_contig16676:32..87Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig16676 33..87 -
1681462874.1776404-CDS-Ecto-sp13_S_contig16676:495..5421681462874.1776404-CDS-Ecto-sp13_S_contig16676:495..542Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig16676 496..542 -
1681462874.185459-CDS-Ecto-sp13_S_contig16676:1208..13761681462874.185459-CDS-Ecto-sp13_S_contig16676:1208..1376Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig16676 1209..1376 -
1681462874.1949832-CDS-Ecto-sp13_S_contig16676:2729..27711681462874.1949832-CDS-Ecto-sp13_S_contig16676:2729..2771Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig16676 2730..2771 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_Ecto-sp13_S_contig16676.4780.1prot_Ecto-sp13_S_contig16676.4780.1Ectocarpus species13 EcNAP12_S_4_19mpolypeptideEcto-sp13_S_contig16676 33..2771 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_Ecto-sp13_S_contig16676.4780.1

>prot_Ecto-sp13_S_contig16676.4780.1 ID=prot_Ecto-sp13_S_contig16676.4780.1|Name=mRNA_Ecto-sp13_S_contig16676.4780.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=polypeptide|length=104bp
EEGRGNIGGDFTLQANPKFLKERSAKFDAIAARQKERLAQKPKLEITVVL
PDGTEKKGVSYETTPMDIAKGISKNLAKEVVVAEVRYSSRIEDDEQNVSS
ADGI
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mRNA from alignment at Ecto-sp13_S_contig16676:31..2771-

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_Ecto-sp13_S_contig16676.4780.1 ID=mRNA_Ecto-sp13_S_contig16676.4780.1|Name=mRNA_Ecto-sp13_S_contig16676.4780.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=mRNA|length=2741bp|location=Sequence derived from alignment at Ecto-sp13_S_contig16676:31..2771- (Ectocarpus species13 EcNAP12_S_4_19m)
GAAGAAGGGAGGGGCAACATCGGCGGCGACTTCACCCTCCAGGCGAGAGA
GCATGCTCGAAGCTCGTAGAATACATCGACACTTTGGACTTCGCGCGCAT
GGTACATACACTCGGAATTTCGTGCGGAGTTGGTGCACAGCAGCAGTACT
ATCACCATGTTTCACGAAACGGTTTGTTTGTTTGGAGGAGACCCAGCCGC
TAGAGGAGAATGCTCGGAATACACACTTTGCTTTCGCGCGCACGATACAC
TCGGAAATTTCGTGTGTATGGACACCAGTACCACGATGTTTTACCCAAAA
TATGCAGCAAAGCATAGATACGACTACCCGTTCGAGGTAACACCACAACG
GAAAGGGGATATTGTGTTGAAGTCTTTGCAGTGAGTGTGAGAAAAAAACG
GCTGGTGCTGCTGATTGCGACACAGTTTTGTTGTTTGAAGGAGACCCTGT
CGCTGGTGGCGAACTGCTGCTTCTGCTAGTAAGCAGGCGGCAGTTGATGT
TAGCGTTGCTGGCAACGACTGCTGCTATCCGATGGATTTTTGGAACCAGG
CGTGCTACTGTAGCCGGTGTTGTAAGCGGACACAGTCTGAATCACCACGT
AATCAACAGCAGTACGTTGTAGTGCTAGTCCCTCTAAATAACACCGGTCG
GTTTGCCGGGCACACAAAAGCGCTAGCTCGTTTGTACCCGGAGGGAAAGA
GAGTTCTGCACTATTGTCTCGCATTTATGGTCGAAGGAAAAATACTTGAG
GAGGGTTGGGTAGGGGACCTCAGGGAGAGGAACCCTTCTGTGTGACCACA
GGTTGGATGAACACGGCACAAACGTGCTGTGAGCGCTTTTTTGAAGTGAA
GACAAGAAGAGAAAAATGGAATATACCGGCACAGGTGACGGCCCCCACTT
GACTCAACTCCGCGTGTGCTCGGTTTGTGCATCAGGCTTTGAATGGTAGG
TGGCATCAACAATTTCTAATATCTCACCCCCTGGAACGATTTTCGACGCT
TGGTTGCTCTACGACGATCACGTTTAATCTCAAACGACACTTTTTCGGAA
AGCTGTCGACGAGACGAGTTCCAACGCCGCCATTTTCAGAACACCGCCGC
GCTCTCTGCCGTGGAGAAATCGAACATTGAAAATGGATCCAAAAGGGGTG
TGTGATACGTTGTGTACCCCCTACAGAGAGGTAACCGCCCTCGCCTTGGG
GTATTCATGTATCCTATGATGGCCAGGTAGCCGCCTTAATTTTTTGGTTG
TGCCCTCCCCCTTTCGTATATCCCACTGGGCTCCGCTCGCTTCGCTGTAG
TGCTCATGCTTGTTGTTTGTGCTTGCCCTCCATCGTCTTACTGCTGCTGC
TGCTGCCGCTGCTGCTGCTGGGATCGATTCCACGACAACAAACAGGCCAA
CCCTAAGTTCCTCAAGGAGCGGTCCGCCAAGTTCGACGCCATCGCCGCCA
GGCAAAAGGAGCGCCTCGCCCAGAAGCCCAAGCTGGAGATCACCGTGGTT
CTCCCCGACGGGACCGAAAAAAAGGGAGTCTCGTACGAGACCACTCCCAT
GGACATCGCGAAGGTGCGCGCGCGGGGTCATGGCACTTGGGTTGGTTGAT
GGGGCCGCCTCCACGAGAAATGTAGTTTATTGAGGGAATGGAACTGGGAG
GGGGTACGCAGACCCTATCCAACCCACTCCCTCGATTGTATGCTTTATCC
AACGTGTTTAATTAGTTATTAGGAAAACCCTGCGAAAGCTACTGGGTTAG
TGTCTGAGGTTTCATCGAACCTTTTCGTTCCTCAGTGTGCTCATGTTTTC
GCACTCGAACACACGCAACGACTTTTTTTGTTCCAGTAGCCTGTGCAAGG
AATGTAAGCTTGGTTATTATTTTGGTAGACGGTTGGTGTCAGGGATAGGG
ATACGAAGTCCCCTCTCTATTCTATAGATTATTTGTGAATCGTGAAATAC
AAAGACGGTCCCTTAGTTCATCAGAGAGGTGGTATTTCATGCGTGTTGTA
GATTCAACTGTCGATAGTAGAGTGTGTAGGTTCTCAGCGAAACCTTCCTC
CCGCCCAGTCTCCGGTCATCATTGACTCGACCTGCCCCCTCCTTGCGAAA
CCCTCCTTGGTTATTGTTGGCGAACGTTGTTTTTTTTCGCGTTCTGGCTG
CTACTCTTTTTTGTTTTTTGTTTTTGCTCTCGTGGCATCATTCTCCCGTA
CCTGCTTGTTCTTTTTCTTTTCTTGTCAGGGCATCAGCAAGAACCTAGCG
AAAGAGGTGGTCGTGGCGGAAGTTCGGTGAGACTTGGTAGACTTTTGGTC
GAGCTTGGCCTTGCTGCCGTATTTTCGTCGTCATCTTCGTGGTGGCTCTT
GTTGCTGGTTTCTTTACTCTTGTTGGACTACCAACTGCTCTTGACCCGTC
GCGGTGACTTTCTTCGCTCGCGCCGTGTTGAAGGGGCTCCGCGACCTTCG
TTGCTCCTTTGCGGGTTTTCCTCTCTCGAGTTAATGCATAGTCACAGCAA
CAAGAAATATGGTACTAGTCATGCATGTGCTTTGCCGATAATTCCCCCTG
CTGCTGCTGCTGCTGTTGTTACCAAGTCAGGTACAACAAAGCATGGTACC
TGCATGTATGCCGACGATTCACCTGCTACATGTATACATGAAAAATGCTT
CACAATTCTCCTCCTGCTGTTGTCGCCATTTCAGGTACTCGTCTCGGATC
GAGGACGACGAGCAGAACGTGTCGAGCGCGGACGGCATAGA
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Coding sequence (CDS) from alignment at Ecto-sp13_S_contig16676:31..2771-

>mRNA_Ecto-sp13_S_contig16676.4780.1 ID=mRNA_Ecto-sp13_S_contig16676.4780.1|Name=mRNA_Ecto-sp13_S_contig16676.4780.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=CDS|length=312bp|location=Sequence derived from alignment at Ecto-sp13_S_contig16676:31..2771- (Ectocarpus species13 EcNAP12_S_4_19m)
GAAGAAGGGAGGGGCAACATCGGCGGCGACTTCACCCTCCAGGCCAACCC
TAAGTTCCTCAAGGAGCGGTCCGCCAAGTTCGACGCCATCGCCGCCAGGC
AAAAGGAGCGCCTCGCCCAGAAGCCCAAGCTGGAGATCACCGTGGTTCTC
CCCGACGGGACCGAAAAAAAGGGAGTCTCGTACGAGACCACTCCCATGGA
CATCGCGAAGGGCATCAGCAAGAACCTAGCGAAAGAGGTGGTCGTGGCGG
AAGTTCGGTACTCGTCTCGGATCGAGGACGACGAGCAGAACGTGTCGAGC
GCGGACGGCATA
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