mRNA_Ecto-sp13_S_contig16531.4701.1 (mRNA) Ectocarpus species13 EcNAP12_S_4_19m

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_Ecto-sp13_S_contig16531.4701.1
Unique NamemRNA_Ecto-sp13_S_contig16531.4701.1
TypemRNA
OrganismEctocarpus species13 EcNAP12_S_4_19m (Ectocarpus species13 EcNAP12_S_4_19m)
Homology
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BLAST of mRNA_Ecto-sp13_S_contig16531.4701.1 vs. uniprot
Match: D7G0P2_ECTSI (Origin recognition complex subunit 4 n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7G0P2_ECTSI)

HSP 1 Score: 491 bits (1263), Expect = 3.060e-170
Identity = 287/310 (92.58%), Postives = 291/310 (93.87%), Query Frame = 1 
Query:    1 QDKTICAAVVGLSVNLNVLDVAEKRVKSRFSFRQISFPRQDLDTIVSILAGALTLPGGSXXXXXXXXXXXXXXXXXXXXXXXAGVSAAFARDFNAVTRQTLGGAKNGVTSANXXXXXXXXXSGAQRCASSALPDLKELAASGRSVGWFLRLASTAVGLLRDDEMGVTRRALARAGEMLAPDSHALALRSLSVTQALVLVAMVRLERRGRKKDGCSFEAVFREYGSYLREFRTCHLRFSRAMIFKAFSQLVEQGALRSAEPGTGGGREAHPVLHCRFRSHFDIEQVERALEKNVIAAPTNVARWCKRWQRQ 930
            QDKTICAAVVGLSVNLNVLDVAEKRVKSRFSFRQI FPRQ LDTIVSILAGALTLPGG+ XXXXXXXXXXXXXXX        GVSAAFARDFNAVTRQTLGGAKNGVTS  XXXXXXXXX   QR ASSALPDLK+LAASGRSVGW LRLASTAVGLLRDDE GVTRRALARAGEMLAPDSH+LALRSLSVTQALVLVAMVRLERRGRKKDGCSFEAVFREYGSYLREFRTCHLRFSRA+IFKAFSQLVEQGALRSAEPGTGGGREAHPVLHCRFRSHFDIEQVERALEKNVIAAPTNVARWCKRWQRQ
Sbjct:  206 QDKTICAAVVGLSVNLNVLDVAEKRVKSRFSFRQIGFPRQALDTIVSILAGALTLPGGNDXXXXXXXXXXXXXXX--------GVSAAFARDFNAVTRQTLGGAKNGVTSXXXXXXXXXXXXXXQRSASSALPDLKDLAASGRSVGWLLRLASTAVGLLRDDEKGVTRRALARAGEMLAPDSHSLALRSLSVTQALVLVAMVRLERRGRKKDGCSFEAVFREYGSYLREFRTCHLRFSRAVIFKAFSQLVEQGALRSAEPGTGGGREAHPVLHCRFRSHFDIEQVERALEKNVIAAPTNVARWCKRWQRQ 507
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BLAST of mRNA_Ecto-sp13_S_contig16531.4701.1 vs. uniprot
Match: A0A6H5JJU9_9PHAE (Origin recognition complex subunit 4 n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JJU9_9PHAE)

HSP 1 Score: 471 bits (1213), Expect = 4.610e-162
Identity = 288/311 (92.60%), Postives = 289/311 (92.93%), Query Frame = 1 
Query:    1 QDKTICAAVVGLSVNLNVLDVAEKRVKSRFSFRQISFPRQDLDTIVSILAGALTLPGGSXXXXXXXXXXXXXXXXXXXXXXXAGVSAAFARDFNAVTRQTLGGAKNGVTSANXXXXXXXXXSG-AQRCASSALPDLKELAASGRSVGWFLRLASTAVGLLRDDEMGVTRRALARAGEMLAPDSHALALRSLSVTQALVLVAMVRLERRGRKKDGCSFEAVFREYGSYLREFRTCHLRFSRAMIFKAFSQLVEQGALRSAEPGTGGGREAHPVLHCRFRSHFDIEQVERALEKNVIAAPTNVARWCKRWQRQ 930
            QDKTICAAVVGLSVNLNVLDVAEKRVKSRFSFRQI FP QDLDTIVSILAGALTLPGGS XXXXXXXXXXXXXXXXXXXXXX       AR+FNAVTRQTLGGAKNG     XXXXXXXXX   AQR ASSALPDLKELAASGRSVGWFLRLASTAVGLLRDDE GVTRRALARAGEMLAPDSHALALRSLSVTQALVLVAMVRLERRGRKKDGCSFE VFREYGSYLREFRTCHLRFSRAMIFKAFSQLVEQGALRSAEPGTGGGREAHPVLHCRFRSHFDIEQVERALEKNVI APTNVARWCKRWQRQ
Sbjct:  244 QDKTICAAVVGLSVNLNVLDVAEKRVKSRFSFRQIGFPGQDLDTIVSILAGALTLPGGSNXXXXXXXXXXXXXXXXXXXXXXX------AREFNAVTRQTLGGAKNGXXXXXXXXXXXXXXXXXAQRSASSALPDLKELAASGRSVGWFLRLASTAVGLLRDDEKGVTRRALARAGEMLAPDSHALALRSLSVTQALVLVAMVRLERRGRKKDGCSFETVFREYGSYLREFRTCHLRFSRAMIFKAFSQLVEQGALRSAEPGTGGGREAHPVLHCRFRSHFDIEQVERALEKNVINAPTNVARWCKRWQRQ 548
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BLAST of mRNA_Ecto-sp13_S_contig16531.4701.1 vs. uniprot
Match: A0A6H5K6K5_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5K6K5_9PHAE)

HSP 1 Score: 108 bits (269), Expect = 8.580e-25
Identity = 49/55 (89.09%), Postives = 50/55 (90.91%), Query Frame = 1 
Query:  766 RSAEPGTGGGREAHPVLHCRFRSHFDIEQVERALEKNVIAAPTNVARWCKRWQRQ 930
            +S E   GGGREAHPVLHCRFRSHFDI QVERALEKNVIAAPTNVARWCKRWQRQ
Sbjct:  138 QSLETLNGGGREAHPVLHCRFRSHFDIGQVERALEKNVIAAPTNVARWCKRWQRQ 192
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BLAST of mRNA_Ecto-sp13_S_contig16531.4701.1 vs. uniprot
Match: A0A835Z3B3_9STRA (Origin recognition complex subunit 4 C-terminus-domain-containing protein n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835Z3B3_9STRA)

HSP 1 Score: 73.2 bits (178), Expect = 8.850e-11
Identity = 48/142 (33.80%), Postives = 70/142 (49.30%), Query Frame = 1 
Query:  496 VTRRALARAGEMLAPDSHALALRSLSVTQALVLVAMVRLERRGRKKDGCSFEAVFREYGSYLREFRTCHLRFSRAMIFKAFSQLVEQGALRSAEPGTGGGREAHPVLHCRFRSHFDIEQVERALEKNVIAAPTNVARWCKRW 921
            +T  A+  A  MLAPD    A++SL   Q LVLVAM+R+ERR  +K   +F  V+ EY S          R++R  I  AF+QL++ G +     G GGG      L        D + +  AL+   +  P  + +WC +W
Sbjct:  344 LTAAAVRAAAHMLAPDHELEAVQSLPQLQLLVLVAMLRMERR--RKRAYTFAGVYAEYESLGARQGLGAARYTRDTIAHAFAQLLQAGLITPVRSGGGGGGAP---LQAIVSLAVDPDALSEALKDGTVECPMAIVQWCTQW 480
The following BLAST results are available for this feature:
BLAST of mRNA_Ecto-sp13_S_contig16531.4701.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 4
Match NameE-valueIdentityDescription
D7G0P2_ECTSI3.060e-17092.58Origin recognition complex subunit 4 n=1 Tax=Ectoc... [more]
A0A6H5JJU9_9PHAE4.610e-16292.60Origin recognition complex subunit 4 n=1 Tax=Ectoc... [more]
A0A6H5K6K5_9PHAE8.580e-2589.09Uncharacterized protein n=1 Tax=Ectocarpus sp. CCA... [more]
A0A835Z3B3_9STRA8.850e-1133.80Origin recognition complex subunit 4 C-terminus-do... [more]
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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
Ecto-sp13_S_contig16531contigEcto-sp13_S_contig16531:449..2976 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Ectocarpus species13 EcNAP12_S_4_19m OGS1.02022-07-08
Properties
Property NameValue
Taxonomic scopeEukaryota
Stop1
Start0
Seed ortholog score491.5
Seed ortholog evalue2.9e-136
Seed eggNOG ortholog2880.D7G0P2
Preferred nameORC4
Model size933
KEGG koko:K02606
KEGG Pathwayko04110,ko04111,ko04113,map04110,map04111,map04113
KEGG ModuleM00284
Hectar predicted targeting categoryother localisation
GOsGO:0000082,GO:0000166,GO:0000228,GO:0000278,GO:0000785,GO:0000790,GO:0000808,GO:0003674,GO:0003676,GO:0003677,GO:0003688,GO:0003690,GO:0003824,GO:0005488,GO:0005524,GO:0005575,GO:0005622,GO:0005623,GO:0005634,GO:0005654,GO:0005656,GO:0005657,GO:0005664,GO:0005694,GO:0005730,GO:0005737,GO:0005829,GO:0006139,GO:0006259,GO:0006260,GO:0006261,GO:0006265,GO:0006267,GO:0006270,GO:0006325,GO:0006342,GO:0006355,GO:0006725,GO:0006807,GO:0006996,GO:0007049,GO:0008144,GO:0008150,GO:0008152,GO:0009058,GO:0009059,GO:0009743,GO:0009744,GO:0009889,GO:0009890,GO:0009892,GO:0009987,GO:0010033,GO:0010468,GO:0010556,GO:0010558,GO:0010605,GO:0010629,GO:0016043,GO:0016458,GO:0016462,GO:0016787,GO:0016817,GO:0016818,GO:0016887,GO:0017076,GO:0017111,GO:0019219,GO:0019222,GO:0022402,GO:0022607,GO:0030466,GO:0030554,GO:0031261,GO:0031323,GO:0031324,GO:0031326,GO:0031327,GO:0031974,GO:0031981,GO:0032553,GO:0032555,GO:0032559,GO:0032991,GO:0032993,GO:0033260,GO:0034285,GO:0034622,GO:0034641,GO:0034645,GO:0035639,GO:0036094,GO:0036387,GO:0036388,GO:0040029,GO:0042221,GO:0043167,GO:0043168,GO:0043170,GO:0043226,GO:0043227,GO:0043228,GO:0043229,GO:0043231,GO:0043232,GO:0043233,GO:0043388,GO:0043565,GO:0043596,GO:0043933,GO:0044085,GO:0044093,GO:0044237,GO:0044238,GO:0044249,GO:0044260,GO:0044422,GO:0044424,GO:0044427,GO:0044428,GO:0044444,GO:0044446,GO:0044451,GO:0044454,GO:0044464,GO:0044770,GO:0044772,GO:0044786,GO:0044843,GO:0045814,GO:0045892,GO:0045934,GO:0046483,GO:0048519,GO:0048523,GO:0050789,GO:0050794,GO:0050896,GO:0051098,GO:0051099,GO:0051101,GO:0051171,GO:0051172,GO:0051252,GO:0051253,GO:0051276,GO:0060255,GO:0065003,GO:0065004,GO:0065007,GO:0065009,GO:0070013,GO:0071103,GO:0071704,GO:0071824,GO:0071840,GO:0080090,GO:0090304,GO:0097159,GO:0097367,GO:1901265,GO:1901360,GO:1901363,GO:1901576,GO:1901700,GO:1902292,GO:1902299,GO:1902315,GO:1902595,GO:1902597,GO:1902679,GO:1902969,GO:1902975,GO:1903047,GO:1903506,GO:1903507,GO:1990837,GO:2000112,GO:2000113,GO:2001141
Exons5
EggNOG free text desc.DNA replication origin binding
EggNOG OGsKOG2228@1,KOG2228@2759
Ec32 ortholog descriptionP-loop containing nucleoside triphosphate hydrolase
Ec32 orthologEc-14_001730.1
Cds size933
COG Functional cat.K
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko00002,ko03032
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1681462868.977698-CDS-Ecto-sp13_S_contig16531:448..5961681462868.977698-CDS-Ecto-sp13_S_contig16531:448..596Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig16531 449..596 -
1681462868.9887056-CDS-Ecto-sp13_S_contig16531:1143..12631681462868.9887056-CDS-Ecto-sp13_S_contig16531:1143..1263Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig16531 1144..1263 -
1681462869.001431-CDS-Ecto-sp13_S_contig16531:1517..16001681462869.001431-CDS-Ecto-sp13_S_contig16531:1517..1600Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig16531 1518..1600 -
1681462869.0144882-CDS-Ecto-sp13_S_contig16531:2060..25401681462869.0144882-CDS-Ecto-sp13_S_contig16531:2060..2540Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig16531 2061..2540 -
1681462869.0239704-CDS-Ecto-sp13_S_contig16531:2874..29761681462869.0239704-CDS-Ecto-sp13_S_contig16531:2874..2976Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig16531 2875..2976 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_Ecto-sp13_S_contig16531.4701.1prot_Ecto-sp13_S_contig16531.4701.1Ectocarpus species13 EcNAP12_S_4_19mpolypeptideEcto-sp13_S_contig16531 449..2976 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_Ecto-sp13_S_contig16531.4701.1

>prot_Ecto-sp13_S_contig16531.4701.1 ID=prot_Ecto-sp13_S_contig16531.4701.1|Name=mRNA_Ecto-sp13_S_contig16531.4701.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=polypeptide|length=311bp
QDKTICAAVVGLSVNLNVLDVAEKRVKSRFSFRQISFPRQDLDTIVSILA
GALTLPGGSDDDDDDDDDDDDDEEGSESPPPPAGVSAAFARDFNAVTRQT
LGGAKNGVTSANNNSGGGGGGSGAQRCASSALPDLKELAASGRSVGWFLR
LASTAVGLLRDDEMGVTRRALARAGEMLAPDSHALALRSLSVTQALVLVA
MVRLERRGRKKDGCSFEAVFREYGSYLREFRTCHLRFSRAMIFKAFSQLV
EQGALRSAEPGTGGGREAHPVLHCRFRSHFDIEQVERALEKNVIAAPTNV
ARWCKRWQRQ*
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mRNA from alignment at Ecto-sp13_S_contig16531:449..2976-

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_Ecto-sp13_S_contig16531.4701.1 ID=mRNA_Ecto-sp13_S_contig16531.4701.1|Name=mRNA_Ecto-sp13_S_contig16531.4701.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=mRNA|length=2528bp|location=Sequence derived from alignment at Ecto-sp13_S_contig16531:449..2976- (Ectocarpus species13 EcNAP12_S_4_19m)
CAGGATAAGACCATTTGCGCGGCTGTGGTGGGCCTCTCGGTCAACCTCAA
CGTCCTGGACGTGGCAGAGAAGAGAGTCAAGTCCAGATTCTCCTTCCGGC
AGGTCAGAGGCACGACAGATAGGATACGGAGATTGGATCAGGGGGGGGGT
GCCGAAGGGAGAACGGACGGAGGGGGGGGGGAGGAGTGGAAGGTTTGGCA
GAGAGTAAAAAGGAGTGATAATACGAGAGGGATAAACAAGCACGGTGTTT
GTACCGAAAGGACAAGGGAGGGGGAGTGGAAGGTTTTCGAAGGGAGAAAG
GGGGAGAGAAAGAAGTATAGAGGTCAAAAGAGGGTTGGACAGGGTGTAGA
GAGTAAGCCTTAGCACAACCGGGTCATGCTACTGGTCTACCCCAACCCAA
CGTCCACTCCCCAACACCCCACCGCGATACCAACAGATAAGCTTCCCGCG
CCAGGACCTCGACACCATCGTTTCGATCCTTGCGGGCGCTCTCACGTTAC
CCGGAGGAAGCGACGACGACGACGATGACGACGACGACGATGACGACGAC
GAAGAGGGCAGCGAATCTCCCCCGCCACCCGCCGGCGTCTCCGCCGCTTT
CGCCCGAGACTTCAACGCCGTCACCCGGCAGACCCTCGGCGGCGCCAAGA
ACGGCGTCACCTCAGCCAACAACAACAGCGGCGGCGGTGGCGGCGGCAGC
GGCGCGCAGCGATGCGCTTCGTCGGCCCTGCCGGATCTGAAGGAACTCGC
GGCATCGGGGAGGAGCGTGGGGTGGTTCTTGAGGCTGGCGTCCACGGCGG
TGGGGCTGCTTAGGGACGACGAGATGGGGGTGACGCGGAGGGCGCTGGCG
AGGGCGGGGGAGATGCTCGCGCCCGACTCGCACGCTCTGGCGCTCCGGAG
CTTGTCGGTGACCCAGGTGGGTGCGTGGAGTGGGGTTGGAGGGAGGGAGG
GAAGGAGGGATTTGCGGCTTTTTGTCAGGACGGAGGGAGAACGGGAGAGG
AGCGGACGGACGCAGGGACGCAGGTAGAGAGGGGAGGTTGTCAGCTGCCG
CCTGAATGCCCCCCACATATTTTTTTTGTCGCCCTGCTGCTGCCGCTGCT
GCTGTAGTCGTTGCGGTATTGTTGTCGCCATTGTGATGATCGTGTTGTGT
TGTTGTTGTGGCATCTAGACGATTGTTTTCTCGCGTGGTTACGACGGCGT
TCGCGCGCTCTCTCTAACGAGAGCACCTGCTGCTGACAATCCTAAGATGG
CGTCGCCGCAAGCTTCGCTTACACCCGCCCGCGTGTTCGTACACGCAATG
ATTTCGCGCATCGCCCACTCTTTGAGACGCACTTCTCCTTTGATTTCGTC
GAAAAAACTCTACCACCCTACGGCAGGCCCTTGTGCTGGTTGCGATGGTG
AGGTTGGAGCGTAGGGGACGCAAGAAGGACGGCTGTTCGTTTGAGGCCGT
TTTTCGAGAGTGAGTGGTGCCATGTGCTGTCCAAATAACTTTACGTGCTC
GTTGTGTTTTCCATTCTTCTAGTGGGGCGGTGTGCTAGTTGATCCTAGCT
TTTCCCCGTTCATGCCTGTTCCCCCGTCTTGCAACATCAAGTGCACTAGG
TTAACGCTAAGCTGTTGCCTGGGTACACTGCACACTCGACGAATCTGTAG
TACACCATCTGAGACCATATGCCCACCCACCCCCACCCCTTGCCCCCGCC
TCACCCCCAAAAGGTACGGGTCGTACCTGCGGGAGTTTCGAACGTGCCAC
TTACGCTTCTCTCGTGCCATGATCTTTAAAGCCTTCAGCCAGCTGGTGGA
GCAAGGCGCTCTGCGATCGGCAGAGCCTGGAACGTGAGAGAGAGAGAGAG
ACGATCTTCTTCTTGACTTCTTGACGTCGGGGGGGTGGGGGTGGGTGGGC
AAGGATGCGATTGCTGACGAAATAAAATGTAGTACATTGCAAAAAACGTA
CATACTACAGCGTATGACCGAGGGGGCACCGTAGGTGGAGTTCTTCCCCG
GTAGGTAGACACTCCCCCGGCCGCACACGGAAGAAGCCGTACCCCCTTCT
TCCAGACCCCCCCCCACTTTCTCGAGGCCCAGACAATTGTTTCACTCTTC
GCGGCTTATCTTTCCTCGCGGAAATTACAACTTTAGTCAAAGGGTAGGAA
CGGTGCTTCTGGGTAAAGAAACTTACGCCGAGTGGGCACGCCCCAGGCCT
CGGGCATCAAGGATCAGAGACTTTGACTTTGAAGTCCGCCCGCATCGTTA
CTTAACAAATCACGGTGCTATGTACATCAAGTCTCAGTCGACCGGATTTG
AGGATATTTTGTATATTGGTTATGGTCGTGCATGAAAGCCAACCCCCCCC
TCTCCCCGTTTGCCGTTGTTTTTTTGAAAGCGGTGGCGGGCGCGAGGCGC
ACCCCGTGCTCCACTGCCGGTTCCGGAGCCATTTCGACATCGAGCAGGTG
GAGCGCGCGCTGGAGAAGAACGTCATTGCCGCCCCCACCAACGTGGCTCG
GTGGTGCAAGAGGTGGCAGAGGCAGTAG
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Coding sequence (CDS) from alignment at Ecto-sp13_S_contig16531:449..2976-

>mRNA_Ecto-sp13_S_contig16531.4701.1 ID=mRNA_Ecto-sp13_S_contig16531.4701.1|Name=mRNA_Ecto-sp13_S_contig16531.4701.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=CDS|length=933bp|location=Sequence derived from alignment at Ecto-sp13_S_contig16531:449..2976- (Ectocarpus species13 EcNAP12_S_4_19m)
CAGGATAAGACCATTTGCGCGGCTGTGGTGGGCCTCTCGGTCAACCTCAA
CGTCCTGGACGTGGCAGAGAAGAGAGTCAAGTCCAGATTCTCCTTCCGGC
AGATAAGCTTCCCGCGCCAGGACCTCGACACCATCGTTTCGATCCTTGCG
GGCGCTCTCACGTTACCCGGAGGAAGCGACGACGACGACGATGACGACGA
CGACGATGACGACGACGAAGAGGGCAGCGAATCTCCCCCGCCACCCGCCG
GCGTCTCCGCCGCTTTCGCCCGAGACTTCAACGCCGTCACCCGGCAGACC
CTCGGCGGCGCCAAGAACGGCGTCACCTCAGCCAACAACAACAGCGGCGG
CGGTGGCGGCGGCAGCGGCGCGCAGCGATGCGCTTCGTCGGCCCTGCCGG
ATCTGAAGGAACTCGCGGCATCGGGGAGGAGCGTGGGGTGGTTCTTGAGG
CTGGCGTCCACGGCGGTGGGGCTGCTTAGGGACGACGAGATGGGGGTGAC
GCGGAGGGCGCTGGCGAGGGCGGGGGAGATGCTCGCGCCCGACTCGCACG
CTCTGGCGCTCCGGAGCTTGTCGGTGACCCAGGCCCTTGTGCTGGTTGCG
ATGGTGAGGTTGGAGCGTAGGGGACGCAAGAAGGACGGCTGTTCGTTTGA
GGCCGTTTTTCGAGAGTACGGGTCGTACCTGCGGGAGTTTCGAACGTGCC
ACTTACGCTTCTCTCGTGCCATGATCTTTAAAGCCTTCAGCCAGCTGGTG
GAGCAAGGCGCTCTGCGATCGGCAGAGCCTGGAACCGGTGGCGGGCGCGA
GGCGCACCCCGTGCTCCACTGCCGGTTCCGGAGCCATTTCGACATCGAGC
AGGTGGAGCGCGCGCTGGAGAAGAACGTCATTGCCGCCCCCACCAACGTG
GCTCGGTGGTGCAAGAGGTGGCAGAGGCAGTAG
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