mRNA_Ecto-sp13_S_contig1647.4664.1 (mRNA) Ectocarpus species13 EcNAP12_S_4_19m

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_Ecto-sp13_S_contig1647.4664.1
Unique NamemRNA_Ecto-sp13_S_contig1647.4664.1
TypemRNA
OrganismEctocarpus species13 EcNAP12_S_4_19m (Ectocarpus species13 EcNAP12_S_4_19m)
Homology
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BLAST of mRNA_Ecto-sp13_S_contig1647.4664.1 vs. uniprot
Match: D7FSV5_ECTSI (Fatty acid desaturase n=2 Tax=Ectocarpus TaxID=2879 RepID=D7FSV5_ECTSI)

HSP 1 Score: 253 bits (645), Expect = 2.090e-80
Identity = 123/133 (92.48%), Postives = 129/133 (96.99%), Query Frame = 1 
Query:    4 LLALHYCLLAALVPLPVAIGHIFLGGWLTATLVTVTHTAEEYAAEDDSSYVETQFRTTRDAITDGPVAEHVWGGMQYQLEHHLFPTIPRYKYRHLVPLVRKFAEDNGIEYRATPQWEIIKDNLALVRSLAHTP 402
            LLALHYCLLAALVPLPVAIGHIFLGGWLTATLVTVTHTAEEYAAEDDSSYVETQFRTTRDAITDGPVAE+VWGGMQYQLEHHLFPTIPRYKYR LVPLVRKFAE+NGIEYRATPQWEIIKDNLAL++ +  +P
Sbjct:  313 LLALHYCLLAALVPLPVAIGHIFLGGWLTATLVTVTHTAEEYAAEDDSSYVETQFRTTRDAITDGPVAEYVWGGMQYQLEHHLFPTIPRYKYRQLVPLVRKFAEENGIEYRATPQWEIIKDNLALLKRVGSSP 445
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BLAST of mRNA_Ecto-sp13_S_contig1647.4664.1 vs. uniprot
Match: D7G2Y4_ECTSI (Fatty acid desaturase n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7G2Y4_ECTSI)

HSP 1 Score: 201 bits (512), Expect = 6.600e-61
Identity = 95/148 (64.19%), Postives = 119/148 (80.41%), Query Frame = 1 
Query:    1 ALLALHYCLLAALVPLPVAIGHIFLGGWLTATLVTVTHTAEEYAAED-------------DSSYVETQFRTTRDAITDGPVAEHVWGGMQYQLEHHLFPTIPRYKYRHLVPLVRKFAEDNGIEYRATPQWEIIKDNLALVRSLAHTPS 405
            ALLALHYC L ALVPLPVAIGH+F+GGWLTAT+VTVTHTAEE++ +D             D+S+VETQF+TTRDA+   P +EH+WGGMQYQLEHHLFPT+PRYKYR LVPLV+KFAE+NG+EY++T  WEII+DN+  ++ +   P+
Sbjct:  257 ALLALHYCWLLALVPLPVAIGHVFVGGWLTATIVTVTHTAEEFSVKDEPAHPSSPFEEEGDASFVETQFKTTRDAVCGDPFSEHLWGGMQYQLEHHLFPTLPRYKYRDLVPLVQKFAEENGLEYKSTGSWEIIRDNIMQLKRVGEAPT 404
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BLAST of mRNA_Ecto-sp13_S_contig1647.4664.1 vs. uniprot
Match: A0A6H5JY43_9PHAE (FA_desaturase domain-containing protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JY43_9PHAE)

HSP 1 Score: 198 bits (503), Expect = 1.300e-60
Identity = 94/148 (63.51%), Postives = 117/148 (79.05%), Query Frame = 1 
Query:    1 ALLALHYCLLAALVPLPVAIGHIFLGGWLTATLVTVTHTAEEYAAED-------------DSSYVETQFRTTRDAITDGPVAEHVWGGMQYQLEHHLFPTIPRYKYRHLVPLVRKFAEDNGIEYRATPQWEIIKDNLALVRSLAHTPS 405
            A LALHYC L ALVPLPVAIGH+F+GGWLTA +VTVTHTAEE++ +D             D+S+VETQF+TTRDA+   P++EH+WGGMQYQLEHHLFPTIPRYKY  LVPLV+KFAE+NG+EYR+T  WEII+DN+  ++ +   P+
Sbjct:  164 AFLALHYCWLLALVPLPVAIGHVFVGGWLTAIIVTVTHTAEEFSVKDEPAHPSSPFEEEGDASFVETQFKTTRDAVCGDPLSEHLWGGMQYQLEHHLFPTIPRYKYPDLVPLVKKFAEENGLEYRSTGSWEIIRDNIKQLKRVGEAPT 311
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BLAST of mRNA_Ecto-sp13_S_contig1647.4664.1 vs. uniprot
Match: A0A836CAU7_9STRA (Fatty acid desaturase n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A836CAU7_9STRA)

HSP 1 Score: 168 bits (425), Expect = 8.670e-48
Identity = 81/133 (60.90%), Postives = 104/133 (78.20%), Query Frame = 1 
Query:    7 LALHYCLLAALVPLPVAIGHIFLGGWLTATLVTVTHTAEEYAAEDDSSYVETQFRTTRDAITDGPVAEHVWGGMQYQLEHHLFPTIPRYKYRHLVPLVRKFAEDNGIEYRATPQWEIIKDNLALVRSLAHTPS 405
            L+LHY  L ALV   VA+GHI LGG LTAT+VTVTHTAEE   +D+ S+VE QFRTTRDA    P++E+VWGGMQYQLEHHLFPT+PRYKY  LVP+++++A +NG+EYR T  W+II DN+  ++ +A  P+
Sbjct:  295 LSLHYVALLALVGPGVALGHIMLGGLLTATIVTVTHTAEEMLMDDEMSFVEAQFRTTRDAKCTDPLSEYVWGGMQYQLEHHLFPTLPRYKYARLVPVIKQWAAENGLEYRVTGAWKIIADNVNQLKRVAAQPT 427
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BLAST of mRNA_Ecto-sp13_S_contig1647.4664.1 vs. uniprot
Match: A0A7S4PM16_GUITH (Hypothetical protein n=1 Tax=Guillardia theta TaxID=55529 RepID=A0A7S4PM16_GUITH)

HSP 1 Score: 145 bits (366), Expect = 1.510e-41
Identity = 68/133 (51.13%), Postives = 95/133 (71.43%), Query Frame = 1 
Query:    7 LALHYCLLAALVPLPVAIGHIFLGGWLTATLVTVTHTAEEYAAEDDSSYVETQFRTTRDAITDGPVAEHVWGGMQYQLEHHLFPTIPRYKYRHLVPLVRKFAEDNGIEYRATPQWEIIKDNLALVRSLAHTPS 405
            LA HY  +   VP    +G +FL G++TA + TVTH +EE   +++  +VE QFR+TRDA+   P +E +WGGMQYQLEHHLFPT+PRY+Y  LVP V+KFAE+NG+E+R TP++E++K N  L   +A  P+
Sbjct:   26 LACHYAFMLYWVPAGTFVGALFLSGFMTAIITTVTHQSEELFFDENPEFVEGQFRSTRDAVCSNPFSEWLWGGMQYQLEHHLFPTMPRYRYPALVPHVKKFAEENGLEFRITPEFELLKMNWKLYSDIAKLPA 158
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BLAST of mRNA_Ecto-sp13_S_contig1647.4664.1 vs. uniprot
Match: A0A7S0LKA8_9EUKA (Hypothetical protein n=1 Tax=Coccolithus braarudii TaxID=221442 RepID=A0A7S0LKA8_9EUKA)

HSP 1 Score: 147 bits (370), Expect = 9.520e-41
Identity = 68/133 (51.13%), Postives = 100/133 (75.19%), Query Frame = 1 
Query:    7 LALHYCLLAALVPLPVAIGHIFLGGWLTATLVTVTHTAEEYAAEDDSSYVETQFRTTRDAITDGPVAEHVWGGMQYQLEHHLFPTIPRYKYRHLVPLVRKFAEDNGIEYRATPQWEIIKDNLALVRSLAHTPS 405
            +AL+Y  +A L+P  VA+GH+FL G ++AT+VTVTH +E+        +V+ Q R+TR+A+T  P++E +WGGMQYQLEHHLFPT+PRYKYR L P+VR++A + G EYR   +W+I+  NLA+++ +AH P+
Sbjct:  156 IALNYLWMALLLPPAVAVGHVFLAGLISATIVTVTHQSEDLFDGLQHDWVDAQLRSTRNAVTSNPLSEWLWGGMQYQLEHHLFPTMPRYKYRALSPIVRRWAAEAGAEYREDGEWQILCRNLAMLQHVAHQPA 288
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BLAST of mRNA_Ecto-sp13_S_contig1647.4664.1 vs. uniprot
Match: L1IL22_GUITC (Uncharacterized protein (Fragment) n=1 Tax=Guillardia theta (strain CCMP2712) TaxID=905079 RepID=L1IL22_GUITC)

HSP 1 Score: 145 bits (366), Expect = 1.620e-39
Identity = 68/133 (51.13%), Postives = 95/133 (71.43%), Query Frame = 1 
Query:    7 LALHYCLLAALVPLPVAIGHIFLGGWLTATLVTVTHTAEEYAAEDDSSYVETQFRTTRDAITDGPVAEHVWGGMQYQLEHHLFPTIPRYKYRHLVPLVRKFAEDNGIEYRATPQWEIIKDNLALVRSLAHTPS 405
            LA HY  +   VP    +G +FL G++TA + TVTH +EE   +++  +VE QFR+TRDA+   P +E +WGGMQYQLEHHLFPT+PRY+Y  LVP V+KFAE+NG+E+R TP++E++K N  L   +A  P+
Sbjct:  246 LACHYAFMLYWVPAGTFVGALFLSGFMTAIITTVTHQSEELFFDENPEFVEGQFRSTRDAVCSNPFSEWLWGGMQYQLEHHLFPTMPRYRYPALVPHVKKFAEENGLEFRITPEFELLKMNWKLYSDIAKLPA 378
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BLAST of mRNA_Ecto-sp13_S_contig1647.4664.1 vs. uniprot
Match: A0A7S3U1W3_9SPIT (Hypothetical protein (Fragment) n=1 Tax=Strombidinopsis acuminata TaxID=141414 RepID=A0A7S3U1W3_9SPIT)

HSP 1 Score: 137 bits (346), Expect = 7.130e-39
Identity = 68/133 (51.13%), Postives = 95/133 (71.43%), Query Frame = 1 
Query:    7 LALHYCLLAALVPLPVAIGHIFLGGWLTATLVTVTHTAEEYAAEDDSSYVETQFRTTRDAITDGPVAEHVWGGMQYQLEHHLFPTIPRYKYRHLVPLVRKFAEDNGIEYRATPQWEIIKDNLALVRSLAHTPS 405
            LAL+Y  LA L P+PVAIGHIFL G ++A +VTVTH +E+        +V  QFR+TR+A T  P +E +WGGMQYQLEHHLFPT+PRY+YR L P ++++A + G+EYR   + E++  N  ++R +AH P+
Sbjct:   28 LALNYLWLACL-PVPVAIGHIFLAGLISAIIVTVTHQSEDMFDGLQHDWVLAQFRSTRNARTSNPFSEWLWGGMQYQLEHHLFPTMPRYRYRRLAPRLKQWAAEQGVEYREDSEAEVLLRNFRMLRDVAHEPA 159
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BLAST of mRNA_Ecto-sp13_S_contig1647.4664.1 vs. uniprot
Match: A0A6U1MJH9_9STRA (Hypothetical protein n=1 Tax=Fibrocapsa japonica TaxID=94617 RepID=A0A6U1MJH9_9STRA)

HSP 1 Score: 142 bits (357), Expect = 1.100e-37
Identity = 72/145 (49.66%), Postives = 96/145 (66.21%), Query Frame = 1 
Query:    7 LALHYCLLAALVPLPVAIGHIFLGGWLTATLVTVTHTAEEYA------------AEDDSSYVETQFRTTRDAITDGPVAEHVWGGMQYQLEHHLFPTIPRYKYRHLVPLVRKFAEDNGIEYRATPQWEIIKDNLALVRSLAHTPS 405
            LA +Y     L+   +  G + LGG L AT+VTVTH AEEY             + DD S+V+ QFRTTRDAI   PV+E++WGGMQYQLEHHLFP +PRYKY  L P+V+++AE  G++YRAT QW+II DN+  ++ +   P+
Sbjct:  300 LAANYAFFLYLLGPAMFAGMVTLGGLLIATIVTVTHQAEEYLPNNDGIGLDGQESSDDPSFVDMQFRTTRDAICRDPVSEYIWGGMQYQLEHHLFPIMPRYKYSKLAPVVKEWAEKVGLDYRATDQWQIISDNINYLKKVGEAPA 444
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BLAST of mRNA_Ecto-sp13_S_contig1647.4664.1 vs. uniprot
Match: A0A7S4DB60_HETAK (Hypothetical protein n=1 Tax=Heterosigma akashiwo TaxID=2829 RepID=A0A7S4DB60_HETAK)

HSP 1 Score: 133 bits (334), Expect = 1.310e-36
Identity = 63/131 (48.09%), Postives = 90/131 (68.70%), Query Frame = 1 
Query:    7 LALHYCLLAALVPLPVAIGHIFLGGWLTATLVTVTHTAEEYAAEDDSSYVETQFRTTRDAITDGPVAEHVWGGMQYQLEHHLFPTIPRYKYRHLVPLVRKFAEDNGIEYRATPQWEIIKDNLALVRSLAHT 399
            +A HY +  AL         + +GG + AT+VT TH +EEY  E + S+V+ QF T+RDA+   P++E+VWGGMQYQLEHHLFPT+PRYKY  LVP+V+++A + GIEYR   ++EI+K N+   + +  T
Sbjct:   52 IAAHYAIFLALFGPGWLFAQVAIGGAMLATIVTCTHQSEEYYEEYEDSFVDNQFSTSRDAVCSNPISEYVWGGMQYQLEHHLFPTMPRYKYPALVPVVQQWAAEQGIEYRTAGEFEIVKRNIDTYKRVGAT 182
The following BLAST results are available for this feature:
BLAST of mRNA_Ecto-sp13_S_contig1647.4664.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
D7FSV5_ECTSI2.090e-8092.48Fatty acid desaturase n=2 Tax=Ectocarpus TaxID=287... [more]
D7G2Y4_ECTSI6.600e-6164.19Fatty acid desaturase n=1 Tax=Ectocarpus siliculos... [more]
A0A6H5JY43_9PHAE1.300e-6063.51FA_desaturase domain-containing protein n=1 Tax=Ec... [more]
A0A836CAU7_9STRA8.670e-4860.90Fatty acid desaturase n=1 Tax=Tribonema minus TaxI... [more]
A0A7S4PM16_GUITH1.510e-4151.13Hypothetical protein n=1 Tax=Guillardia theta TaxI... [more]
A0A7S0LKA8_9EUKA9.520e-4151.13Hypothetical protein n=1 Tax=Coccolithus braarudii... [more]
L1IL22_GUITC1.620e-3951.13Uncharacterized protein (Fragment) n=1 Tax=Guillar... [more]
A0A7S3U1W3_9SPIT7.130e-3951.13Hypothetical protein (Fragment) n=1 Tax=Strombidin... [more]
A0A6U1MJH9_9STRA1.100e-3749.66Hypothetical protein n=1 Tax=Fibrocapsa japonica T... [more]
A0A7S4DB60_HETAK1.310e-3648.09Hypothetical protein n=1 Tax=Heterosigma akashiwo ... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
Ecto-sp13_S_contig1647contigEcto-sp13_S_contig1647:15122..16101 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Ectocarpus species13 EcNAP12_S_4_19m OGS1.02022-07-08
Properties
Property NameValue
Taxonomic scopeEukaryota
Stop0
Start0
Seed ortholog score255.0
Seed ortholog evalue2e-65
Seed eggNOG ortholog2880.D7FSV5
Model size405
KEGG koko:K21737
Hectar predicted targeting categorysignal peptide
GOsGO:0000003,GO:0001505,GO:0001820,GO:0002682,GO:0003008,GO:0003012,GO:0003674,GO:0003824,GO:0004768,GO:0005575,GO:0006082,GO:0006629,GO:0006631,GO:0006633,GO:0006636,GO:0006810,GO:0006811,GO:0006812,GO:0006836,GO:0006837,GO:0006936,GO:0007154,GO:0007267,GO:0007268,GO:0007269,GO:0007271,GO:0007275,GO:0007588,GO:0007610,GO:0007631,GO:0008104,GO:0008150,GO:0008152,GO:0008610,GO:0009058,GO:0009914,GO:0009987,GO:0010817,GO:0014055,GO:0015695,GO:0015696,GO:0015844,GO:0015850,GO:0015870,GO:0015893,GO:0016020,GO:0016021,GO:0016053,GO:0016192,GO:0016197,GO:0016215,GO:0016491,GO:0016705,GO:0016717,GO:0018991,GO:0019098,GO:0019752,GO:0022414,GO:0023052,GO:0023061,GO:0030421,GO:0031224,GO:0031347,GO:0032456,GO:0032501,GO:0032502,GO:0032504,GO:0032787,GO:0032940,GO:0033036,GO:0033559,GO:0035264,GO:0040002,GO:0040007,GO:0040011,GO:0042221,GO:0042335,GO:0042493,GO:0042755,GO:0043050,GO:0043436,GO:0044237,GO:0044238,GO:0044249,GO:0044255,GO:0044281,GO:0044283,GO:0044425,GO:0045088,GO:0046394,GO:0046879,GO:0046903,GO:0046907,GO:0048583,GO:0048589,GO:0048609,GO:0048856,GO:0050776,GO:0050789,GO:0050896,GO:0051179,GO:0051234,GO:0051641,GO:0051649,GO:0055114,GO:0061526,GO:0065007,GO:0065008,GO:0071702,GO:0071704,GO:0071705,GO:0072330,GO:0080134,GO:0098916,GO:0099536,GO:0099537,GO:0099643,GO:1901374,GO:1901576
Exons2
EggNOG free text desc.unsaturated fatty acid biosynthetic process
EggNOG OGsCOG3239@1,KOG4232@2759
EC1.14.19.38,1.14.19.47
Cds size405
COG Functional cat.I
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko01000
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1681462866.0422623-CDS-Ecto-sp13_S_contig1647:15121..153171681462866.0422623-CDS-Ecto-sp13_S_contig1647:15121..15317Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig1647 15122..15317 -
1681462866.0531855-CDS-Ecto-sp13_S_contig1647:15892..161011681462866.0531855-CDS-Ecto-sp13_S_contig1647:15892..16101Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig1647 15893..16101 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_Ecto-sp13_S_contig1647.4664.1prot_Ecto-sp13_S_contig1647.4664.1Ectocarpus species13 EcNAP12_S_4_19mpolypeptideEcto-sp13_S_contig1647 15122..16101 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_Ecto-sp13_S_contig1647.4664.1

>prot_Ecto-sp13_S_contig1647.4664.1 ID=prot_Ecto-sp13_S_contig1647.4664.1|Name=mRNA_Ecto-sp13_S_contig1647.4664.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=polypeptide|length=135bp
ALLALHYCLLAALVPLPVAIGHIFLGGWLTATLVTVTHTAEEYAAEDDSS
YVETQFRTTRDAITDGPVAEHVWGGMQYQLEHHLFPTIPRYKYRHLVPLV
RKFAEDNGIEYRATPQWEIIKDNLALVRSLAHTPS
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mRNA from alignment at Ecto-sp13_S_contig1647:15122..16101-

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_Ecto-sp13_S_contig1647.4664.1 ID=mRNA_Ecto-sp13_S_contig1647.4664.1|Name=mRNA_Ecto-sp13_S_contig1647.4664.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=mRNA|length=980bp|location=Sequence derived from alignment at Ecto-sp13_S_contig1647:15122..16101- (Ectocarpus species13 EcNAP12_S_4_19m)
GCTCTCCTAGCGCTGCACTACTGCTTGCTCGCGGCCCTGGTGCCCCTTCC
GGTGGCGATCGGCCACATTTTCCTCGGGGGGTGGCTTACGGCTACCCTCG
TGACGGTGACACACACGGCGGAGGAGTACGCCGCCGAGGACGACTCGTCG
TACGTGGAGACGCAGTTCAGGACCACTAGGGACGCCATCACCGATGGACC
CGTCGCGGAGTGAGTTTCGCCTTTTTCTTTATTTTCCCTGCTCTCGTCGT
CTCGTTCTTAATTTTTGTTCCTGGTTCCGTTCTCGAGGCATTACTTTTAA
TCTGCCTCCTTTGCCGTGATTTTTGTTGCAGGGTCGGTTCTTGATTTTTT
TTTTTTTCAAAGTCAAGGAAACGGGCTCCCTCTCTTGCGCCCATCTCTGT
GCTGGTTTTCTTTCAAATGTTGTCTGATTTGATTAGTTATGCGGGACGCC
TTTTCACCCGAAAGACAAGTATGGATGACGGAAGCAATCGCCATATTTTT
TATCGTGATCTCTCTGCCAGCAGTATGTGCAGCCGACAGCCCCGTCTACG
TCTTTCGTGCCTTCCATATATTGTTATGGTCATGTTCCTAACCTCTATCC
TTCGATTGCCCCTCTTTCAGTCCCCCAGTTGGTTGTGACAAACGGGAAAT
CACCTCATCTAAAATGGTACGGTTCTGGGTGCCTATCCCTTGCACTGACG
TCATCATCTCTGCCCCCCCTTCTCCGCCTTTTGACTTGTATGGGTGACTA
CAACGACAACAATCGTACCATGTTTCTCTACCAGACACGTGTGGGGCGGC
ATGCAGTACCAGCTGGAGCACCACCTGTTCCCTACCATCCCCCGGTACAA
ATACCGCCACCTCGTGCCCCTCGTGAGGAAGTTCGCCGAGGATAACGGTA
TCGAGTACAGGGCCACCCCCCAGTGGGAGATCATCAAGGACAACTTGGCT
CTGGTAAGGTCGTTGGCTCACACGCCTTCT
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Coding sequence (CDS) from alignment at Ecto-sp13_S_contig1647:15122..16101-

>mRNA_Ecto-sp13_S_contig1647.4664.1 ID=mRNA_Ecto-sp13_S_contig1647.4664.1|Name=mRNA_Ecto-sp13_S_contig1647.4664.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=CDS|length=405bp|location=Sequence derived from alignment at Ecto-sp13_S_contig1647:15122..16101- (Ectocarpus species13 EcNAP12_S_4_19m)
GCTCTCCTAGCGCTGCACTACTGCTTGCTCGCGGCCCTGGTGCCCCTTCC
GGTGGCGATCGGCCACATTTTCCTCGGGGGGTGGCTTACGGCTACCCTCG
TGACGGTGACACACACGGCGGAGGAGTACGCCGCCGAGGACGACTCGTCG
TACGTGGAGACGCAGTTCAGGACCACTAGGGACGCCATCACCGATGGACC
CGTCGCGGAACACGTGTGGGGCGGCATGCAGTACCAGCTGGAGCACCACC
TGTTCCCTACCATCCCCCGGTACAAATACCGCCACCTCGTGCCCCTCGTG
AGGAAGTTCGCCGAGGATAACGGTATCGAGTACAGGGCCACCCCCCAGTG
GGAGATCATCAAGGACAACTTGGCTCTGGTAAGGTCGTTGGCTCACACGC
CTTCT
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