mRNA_Ecto-sp13_S_contig16240.4528.1 (mRNA) Ectocarpus species13 EcNAP12_S_4_19m

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_Ecto-sp13_S_contig16240.4528.1
Unique NamemRNA_Ecto-sp13_S_contig16240.4528.1
TypemRNA
OrganismEctocarpus species13 EcNAP12_S_4_19m (Ectocarpus species13 EcNAP12_S_4_19m)
Homology
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BLAST of mRNA_Ecto-sp13_S_contig16240.4528.1 vs. uniprot
Match: A0A6H5JWE1_9PHAE (B30.2/SPRY domain-containing protein n=2 Tax=Ectocarpus TaxID=2879 RepID=A0A6H5JWE1_9PHAE)

HSP 1 Score: 156 bits (394), Expect = 3.370e-41
Identity = 81/95 (85.26%), Postives = 85/95 (89.47%), Query Frame = 1 
Query:    1 QWWMLVSNGECPSAVTENAPGVTRTLANSPGTVLRSNATKEAPPQCKIDGVLSALSDLEEAGLKQEEMDEVLADARFVAPLSSGNSAELVRGGLD 285
            QWWMLVSNGECPSAV EN PGVTRTLA+S  TVL SNATKEAPP+  IDGV+SALS LEEAGLKQEEMDEVLADARFVAPLSSG+S ELVRGG D
Sbjct: 2033 QWWMLVSNGECPSAVPENEPGVTRTLADSARTVLCSNATKEAPPKYTIDGVISALSHLEEAGLKQEEMDEVLADARFVAPLSSGDSTELVRGGED 2127
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BLAST of mRNA_Ecto-sp13_S_contig16240.4528.1 vs. uniprot
Match: A0A484EBI2_BRELC (Uncharacterized protein n=1 Tax=Bremia lactucae TaxID=4779 RepID=A0A484EBI2_BRELC)

HSP 1 Score: 60.1 bits (144), Expect = 1.370e-7
Identity = 48/143 (33.57%), Postives = 65/143 (45.45%), Query Frame = 1 
Query:   34 PSAVTENAPGVTRTLANSPGTVLRSNATKEAPPQCKIDGVLSALSDLEEAGLKQEEMDEVLA--DARFVAPLSSGNSAELVRGGLDRRVTNQNLGEYRARLARLRALEYASQAAAFRAGMEAALPNRVLSLLTWRELEQIVCG 456
            PS V +   G T T A+   T        +    C+ DG+L+           +EE     A  + R+ A   +G   ELV GG+ R+V   N  EY     R R  E ++Q AA   G     P RVL+LLTW+ELE + CG
Sbjct: 4792 PSLVWKQLLGQTLTRADLEDTDAMFIQMLDGIAHCESDGILT-----------EEEFATAFAGLELRYTASSCTGEEIELVPGGITRKVDFLNRLEYCRLAERARLEECSAQVAAMARGFATLFPRRVLTLLTWQELEILACG 4923
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BLAST of mRNA_Ecto-sp13_S_contig16240.4528.1 vs. uniprot
Match: UPI001BB22953 (probable E3 ubiquitin-protein ligase HERC1 isoform X3 n=1 Tax=Lytechinus variegatus TaxID=7654 RepID=UPI001BB22953)

HSP 1 Score: 60.1 bits (144), Expect = 1.370e-7
Identity = 38/101 (37.62%), Postives = 56/101 (55.45%), Query Frame = 1 
Query:  154 LSALSDLEEAGLKQEEMDEVLADARFVAPLSSGNSAELVRGGLDRRVTNQNLGEYRARLARLRALEYASQAAAFRAGMEAALPNRVLSLLTWRELEQIVCG 456
            L ++SD++++G+ ++   EV+   RF    + G    +V GG    +T  N  EY  R    R  E   Q AA R GM   +P  +LSLLT+R+LEQ+VCG
Sbjct: 4941 LRSISDIDKSGVNEDNFHEVIPLERFEGQSADGRFVPVVPGGWSIPLTFSNRKEYVERALAYRLHELDRQVAAVREGMSWIIPIPLLSLLTYRQLEQMVCG 5041
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BLAST of mRNA_Ecto-sp13_S_contig16240.4528.1 vs. uniprot
Match: UPI001BB27410 (probable E3 ubiquitin-protein ligase HERC1 isoform X2 n=1 Tax=Lytechinus variegatus TaxID=7654 RepID=UPI001BB27410)

HSP 1 Score: 60.1 bits (144), Expect = 1.370e-7
Identity = 38/101 (37.62%), Postives = 56/101 (55.45%), Query Frame = 1 
Query:  154 LSALSDLEEAGLKQEEMDEVLADARFVAPLSSGNSAELVRGGLDRRVTNQNLGEYRARLARLRALEYASQAAAFRAGMEAALPNRVLSLLTWRELEQIVCG 456
            L ++SD++++G+ ++   EV+   RF    + G    +V GG    +T  N  EY  R    R  E   Q AA R GM   +P  +LSLLT+R+LEQ+VCG
Sbjct: 4941 LRSISDIDKSGVNEDNFHEVIPLERFEGQSADGRFVPVVPGGWSIPLTFSNRKEYVERALAYRLHELDRQVAAVREGMSWIIPIPLLSLLTYRQLEQMVCG 5041
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BLAST of mRNA_Ecto-sp13_S_contig16240.4528.1 vs. uniprot
Match: UPI001BB143C8 (probable E3 ubiquitin-protein ligase HERC1 isoform X1 n=2 Tax=Lytechinus variegatus TaxID=7654 RepID=UPI001BB143C8)

HSP 1 Score: 60.1 bits (144), Expect = 1.370e-7
Identity = 38/101 (37.62%), Postives = 56/101 (55.45%), Query Frame = 1 
Query:  154 LSALSDLEEAGLKQEEMDEVLADARFVAPLSSGNSAELVRGGLDRRVTNQNLGEYRARLARLRALEYASQAAAFRAGMEAALPNRVLSLLTWRELEQIVCG 456
            L ++SD++++G+ ++   EV+   RF    + G    +V GG    +T  N  EY  R    R  E   Q AA R GM   +P  +LSLLT+R+LEQ+VCG
Sbjct: 4942 LRSISDIDKSGVNEDNFHEVIPLERFEGQSADGRFVPVVPGGWSIPLTFSNRKEYVERALAYRLHELDRQVAAVREGMSWIIPIPLLSLLTYRQLEQMVCG 5042
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BLAST of mRNA_Ecto-sp13_S_contig16240.4528.1 vs. uniprot
Match: UPI001BB2B9BD (LOW QUALITY PROTEIN: probable E3 ubiquitin-protein ligase HERC1 n=1 Tax=Lytechinus variegatus TaxID=7654 RepID=UPI001BB2B9BD)

HSP 1 Score: 60.1 bits (144), Expect = 1.370e-7
Identity = 38/101 (37.62%), Postives = 56/101 (55.45%), Query Frame = 1 
Query:  154 LSALSDLEEAGLKQEEMDEVLADARFVAPLSSGNSAELVRGGLDRRVTNQNLGEYRARLARLRALEYASQAAAFRAGMEAALPNRVLSLLTWRELEQIVCG 456
            L ++SD++++G+ ++   EV+   RF    + G    +V GG    +T  N  EY  R    R  E   Q AA R GM   +P  +LSLLT+R+LEQ+VCG
Sbjct: 4949 LRSISDIDKSGVNEDNFHEVIPLERFEGQSADGRFVPVVPGGWSIPLTFSNRKEYVERALAYRLHELDRQVAAVREGMSWIIPIPLLSLLTYRQLEQMVCG 5049
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BLAST of mRNA_Ecto-sp13_S_contig16240.4528.1 vs. uniprot
Match: A0A7M7SUN9_STRPU (Uncharacterized protein n=3 Tax=Strongylocentrotus purpuratus TaxID=7668 RepID=A0A7M7SUN9_STRPU)

HSP 1 Score: 59.3 bits (142), Expect = 2.530e-7
Identity = 38/101 (37.62%), Postives = 55/101 (54.46%), Query Frame = 1 
Query:  154 LSALSDLEEAGLKQEEMDEVLADARFVAPLSSGNSAELVRGGLDRRVTNQNLGEYRARLARLRALEYASQAAAFRAGMEAALPNRVLSLLTWRELEQIVCG 456
            L  +SD++++G+ ++   EV+   RF    + G    +V GG    +T  N  EY  R    R  E   Q AA R GM   +P  +LSLLT+R+LEQ+VCG
Sbjct: 4943 LRNISDIDKSGVNEDNFHEVIPLERFEGQSADGRFVPIVPGGWSIPLTFSNRKEYVERALAYRLHELDRQVAAVREGMSWIIPIPLLSLLTYRQLEQMVCG 5043
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BLAST of mRNA_Ecto-sp13_S_contig16240.4528.1 vs. uniprot
Match: A0A8K1FMX5_PYTOL (Uncharacterized protein n=1 Tax=Pythium oligandrum TaxID=41045 RepID=A0A8K1FMX5_PYTOL)

HSP 1 Score: 58.5 bits (140), Expect = 4.630e-7
Identity = 40/105 (38.10%), Postives = 52/105 (49.52%), Query Frame = 1 
Query:  151 VLSALSDLEEAGLKQEEMDEVL---ADARFVAPLSSGNSAELVRGGLDRRVTNQNLGEYRARLARLRALEYASQAAAFRAGMEAALPNRVLSLLTWRELEQIVCG 456
            +L  + + E  G+  E+  E      D RF A   +G   ELV GG   RVT +N  EY     + R  E A Q AA   G+    P RVL+LLTW E+E + CG
Sbjct: 3208 MLDGIQNCERDGIMTEDEFETAFEGLDLRFTAFDCNGQEIELVEGGKSTRVTFENRLEYCQLAEQYRLQEGAKQVAAMARGLATIFPVRVLTLLTWHEMEMLTCG 3312
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BLAST of mRNA_Ecto-sp13_S_contig16240.4528.1 vs. uniprot
Match: A0A7S2TPF8_9EUKA (HECT-type E3 ubiquitin transferase n=1 Tax=Lotharella oceanica TaxID=641309 RepID=A0A7S2TPF8_9EUKA)

HSP 1 Score: 57.0 bits (136), Expect = 7.290e-7
Identity = 32/88 (36.36%), Postives = 46/88 (52.27%), Query Frame = 1 
Query:  193 QEEMDEVLADARFVAPLSSGNSAELVRGGLDRRVTNQNLGEYRARLARLRALEYASQAAAFRAGMEAALPNRVLSLLTWRELEQIVCG 456
            +E  D+ + D RF    S GN+ EL+RGG    V  +N   Y       +  E  +Q  A R G+   +P ++LSL TWR+L+  VCG
Sbjct:    4 KEAFDKAVGDVRFAVRSSEGNAVELLRGGSAIHVNIENADTYINLYRDYKLNETRTQCEAMRRGLATVIPYQILSLFTWRDLQDQVCG 91
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BLAST of mRNA_Ecto-sp13_S_contig16240.4528.1 vs. uniprot
Match: A0A6P6KLZ9_CARAU (probable E3 ubiquitin-protein ligase HERC1 isoform X6 n=1 Tax=Carassius auratus TaxID=7957 RepID=A0A6P6KLZ9_CARAU)

HSP 1 Score: 56.2 bits (134), Expect = 2.950e-6
Identity = 37/101 (36.63%), Postives = 54/101 (53.47%), Query Frame = 1 
Query:  154 LSALSDLEEAGLKQEEMDEVLADARFVAPLSSGNSAELVRGGLDRRVTNQNLGEYRARLARLRALEYASQAAAFRAGMEAALPNRVLSLLTWRELEQIVCG 456
            LS++  LE++G+ ++   E++    FV   + G    ++ GG    +T  N  EY  R    R  E   Q AA R GM   +P  +LSLLT R+LEQ+VCG
Sbjct: 4625 LSSILHLEDSGITEQNFHEMIPLDSFVGQSADGKMVPIIPGGNSIPLTFSNRKEYVERAIEYRLHEMERQVAAVREGMSWIVPVPLLSLLTARQLEQMVCG 4725
The following BLAST results are available for this feature:
BLAST of mRNA_Ecto-sp13_S_contig16240.4528.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A6H5JWE1_9PHAE3.370e-4185.26B30.2/SPRY domain-containing protein n=2 Tax=Ectoc... [more]
A0A484EBI2_BRELC1.370e-733.57Uncharacterized protein n=1 Tax=Bremia lactucae Ta... [more]
UPI001BB229531.370e-737.62probable E3 ubiquitin-protein ligase HERC1 isoform... [more]
UPI001BB274101.370e-737.62probable E3 ubiquitin-protein ligase HERC1 isoform... [more]
UPI001BB143C81.370e-737.62probable E3 ubiquitin-protein ligase HERC1 isoform... [more]
UPI001BB2B9BD1.370e-737.62LOW QUALITY PROTEIN: probable E3 ubiquitin-protein... [more]
A0A7M7SUN9_STRPU2.530e-737.62Uncharacterized protein n=3 Tax=Strongylocentrotus... [more]
A0A8K1FMX5_PYTOL4.630e-738.10Uncharacterized protein n=1 Tax=Pythium oligandrum... [more]
A0A7S2TPF8_9EUKA7.290e-736.36HECT-type E3 ubiquitin transferase n=1 Tax=Lothare... [more]
A0A6P6KLZ9_CARAU2.950e-636.63probable E3 ubiquitin-protein ligase HERC1 isoform... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
Ecto-sp13_S_contig16240contigEcto-sp13_S_contig16240:883..1867 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Ectocarpus species13 EcNAP12_S_4_19m OGS1.02022-07-08
Properties
Property NameValue
Taxonomic scopeEukaryota
Stop1
Start1
Seed ortholog score253.1
Seed ortholog evalue8.4e-65
Seed eggNOG ortholog2880.D7G597
Model size459
KEGG koko:K12169
Hectar predicted targeting categoryother localisation
Exons2
EggNOG free text desc.RNA localization to chromatin
EggNOG OGsCOG5184@1,KOG1426@2759,KOG2242@1,KOG2242@2759
EC2.3.2.27
Cds size450
COG Functional cat.L
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko01000,ko04121
Relationships

The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1681462855.60462-UTR-Ecto-sp13_S_contig16240:882..8911681462855.60462-UTR-Ecto-sp13_S_contig16240:882..891Ectocarpus species13 EcNAP12_S_4_19mUTREcto-sp13_S_contig16240 883..891 +


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1681462855.6144874-CDS-Ecto-sp13_S_contig16240:891..11591681462855.6144874-CDS-Ecto-sp13_S_contig16240:891..1159Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig16240 892..1159 +
1681462855.6271696-CDS-Ecto-sp13_S_contig16240:1685..18671681462855.6271696-CDS-Ecto-sp13_S_contig16240:1685..1867Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig16240 1686..1867 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_Ecto-sp13_S_contig16240.4528.1prot_Ecto-sp13_S_contig16240.4528.1Ectocarpus species13 EcNAP12_S_4_19mpolypeptideEcto-sp13_S_contig16240 892..1867 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_Ecto-sp13_S_contig16240.4528.1

>prot_Ecto-sp13_S_contig16240.4528.1 ID=prot_Ecto-sp13_S_contig16240.4528.1|Name=mRNA_Ecto-sp13_S_contig16240.4528.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=polypeptide|length=150bp
MLVSNGECPSAVTENAPGVTRTLANSPGTVLRSNATKEAPPQCKIDGVLS
ALSDLEEAGLKQEEMDEVLADARFVAPLSSGNSAELVRGGLDRRVTNQNL
GEYRARLARLRALEYASQAAAFRAGMEAALPNRVLSLLTWRELEQIVCG*
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mRNA from alignment at Ecto-sp13_S_contig16240:883..1867+

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_Ecto-sp13_S_contig16240.4528.1 ID=mRNA_Ecto-sp13_S_contig16240.4528.1|Name=mRNA_Ecto-sp13_S_contig16240.4528.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=mRNA|length=985bp|location=Sequence derived from alignment at Ecto-sp13_S_contig16240:883..1867+ (Ectocarpus species13 EcNAP12_S_4_19m)
CAGTGGTGGATGCTCGTTTCAAACGGCGAATGTCCGTCGGCGGTCACTGA
AAATGCACCGGGAGTGACCAGGACGCTTGCAAATTCTCCGGGGACGGTGT
TGCGTTCGAACGCCACAAAAGAAGCACCGCCACAATGTAAAATAGACGGG
GTACTTTCGGCCCTCAGCGACCTTGAGGAAGCGGGCTTGAAGCAAGAAGA
AATGGACGAAGTGCTCGCGGATGCAAGATTCGTTGCTCCGCTGTCAAGCG
GGAATTCCGCGGAACTCGTTCGCGGAGGTGAGAACTGGCGGCAAGGGTCG
GTTGAATAGTTTCCACTTTTCATCGACGACACCGGCTCACTCCCTCTCGA
ACCGTCCAAACTTTTCTCAACGCTACCACAAAGACGACTTTACGAGCGTG
CGGGCACATGTTTCGATCTGTTAGCGTATCCACGGTATGTAGTAGCAGTC
AACGAAGACCCACTTGTTGTCTTTTCTTGCGATCGTGGTTCGTCATGTTA
TTTTGGAGCCAAATAGACGGAATGCATGTGATGGGAGTCGTTATTCTAGC
ACCTTTCTCTTGCAGATGGAAATTCGATGGTAGTTGTGTTTTGGGTACTC
GGTGGTGGTGTTTCGCCAGAACGAAACGAAGGCAGACCACTGTATAATGT
TGTAAGCTTCAAATTGTGACTGACTCACCAAGGCGTGTAAAACGCATGCA
TACCCGTATTTCCCAACAATAATTACTTAGCGGTTGCTGCAATTCACACC
CATCCCCCGTCCTCCCTTTTCGTTGCTGCTACATTTTGGATGGTTCTCGC
CAGGTCTTGATCGTCGCGTGACCAATCAAAATCTCGGCGAATATCGCGCC
CGTTTGGCCAGGTTACGAGCCTTGGAATACGCTTCACAGGCAGCCGCCTT
TCGGGCGGGCATGGAGGCCGCGCTTCCCAATCGGGTGCTTTCGTTGTTGA
CGTGGAGGGAATTGGAACAGATTGTTTGTGGGTAG
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Coding sequence (CDS) from alignment at Ecto-sp13_S_contig16240:883..1867+

>mRNA_Ecto-sp13_S_contig16240.4528.1 ID=mRNA_Ecto-sp13_S_contig16240.4528.1|Name=mRNA_Ecto-sp13_S_contig16240.4528.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=CDS|length=450bp|location=Sequence derived from alignment at Ecto-sp13_S_contig16240:883..1867+ (Ectocarpus species13 EcNAP12_S_4_19m)
ATGCTCGTTTCAAACGGCGAATGTCCGTCGGCGGTCACTGAAAATGCACC
GGGAGTGACCAGGACGCTTGCAAATTCTCCGGGGACGGTGTTGCGTTCGA
ACGCCACAAAAGAAGCACCGCCACAATGTAAAATAGACGGGGTACTTTCG
GCCCTCAGCGACCTTGAGGAAGCGGGCTTGAAGCAAGAAGAAATGGACGA
AGTGCTCGCGGATGCAAGATTCGTTGCTCCGCTGTCAAGCGGGAATTCCG
CGGAACTCGTTCGCGGAGGTCTTGATCGTCGCGTGACCAATCAAAATCTC
GGCGAATATCGCGCCCGTTTGGCCAGGTTACGAGCCTTGGAATACGCTTC
ACAGGCAGCCGCCTTTCGGGCGGGCATGGAGGCCGCGCTTCCCAATCGGG
TGCTTTCGTTGTTGACGTGGAGGGAATTGGAACAGATTGTTTGTGGGTAG
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