mRNA_Ecto-sp13_S_contig16138.4475.1 (mRNA) Ectocarpus species13 EcNAP12_S_4_19m

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_Ecto-sp13_S_contig16138.4475.1
Unique NamemRNA_Ecto-sp13_S_contig16138.4475.1
TypemRNA
OrganismEctocarpus species13 EcNAP12_S_4_19m (Ectocarpus species13 EcNAP12_S_4_19m)
Homology
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BLAST of mRNA_Ecto-sp13_S_contig16138.4475.1 vs. uniprot
Match: D7FKH8_ECTSI (Peptide n-glycanase, putative n=2 Tax=Ectocarpus TaxID=2879 RepID=D7FKH8_ECTSI)

HSP 1 Score: 828 bits (2138), Expect = 2.160e-288
Identity = 450/469 (95.95%), Postives = 453/469 (96.59%), Query Frame = 1 
Query:   22 TPTAIAVVGLKKGPTGARRWVRRQHRGEDGGGGRCALYAEILLPPPASGRAAAVQVELQDKLSLCPLRSLPAEGDAVAATTTPSAAPVSAPARGTCRRGGEPTVSVGPAVDLVDCPGWTTVLQVPGDMAGVASADDAGKEEVSVVPPVGWVVTTVHPAVGGMHEDTEEFDDGPVGCPAFVMGGSPVALTADGSEPPFLPAGRVREIVGWSGDAVNGVQVVYDVQGDAVRGPKRMGDHGLYRQSNFVLDVEGGEVLTEISVKAGAIVDSLRVRTNKGREKTWGGAGGHLQRTWHVPTGSSFLGFHGGVGGHVHSLGVTLAERGGQAAGASGETSLALDPVVKTNLYAADRVARACAQLLAFNAPXXXXXXXXXXXXXXXXXXXXXXXXXXALEEVVTALETMRKYADNLLASPLDPKVSRIRLANGFFDRKIGRLAGGGGVVRAMGFGLADEGGRMHYVFRRQGAGGGLG 1428
            TPTAIAVVGLKKGPTGARRW RRQHRGEDGGGGRCALY EILLPPPASGRAAAVQVELQDKLSLCPLRSLPAEGDA AATTTPSA  VSA ARGTCRR GEPTVS+GPAVDLVDCPGWTTVLQVPGDMAG  SADDA KEEVS VPPVGWVVTTVHPAVGG HEDTEEFDDGPVGCPAFVMGGSPVALTADGS PPFLPAGRVREIVGWSGDAVNGVQVVYDVQGDAVRGPKRMGDHGLYRQSNFVLDVEGGEVLTEISVKAGAIVDS+RVRTNKGREKTWGGAGGHLQRTWHVPTGSSFLGFHGGVGGHVHSLGVTLAERGGQAAGASGE+SLALDPVVKTNLYAADRVARACAQ LAFNAPXXXXXXXXXXXXXXXXXXXXXXXXXXALEEVVTALETMRKYADNLLASPLDPKVSRIRLANGFFDRKIGRLAGGGGVVRAMGF LADEGGRMHYVFRRQG GGGLG
Sbjct:  846 TPTAIAVVGLKKGPTGARRWARRQHRGEDGGGGRCALYTEILLPPPASGRAAAVQVELQDKLSLCPLRSLPAEGDAAAATTTPSAVAVSASARGTCRRAGEPTVSMGPAVDLVDCPGWTTVLQVPGDMAGAGSADDAEKEEVSAVPPVGWVVTTVHPAVGGTHEDTEEFDDGPVGCPAFVMGGSPVALTADGSAPPFLPAGRVREIVGWSGDAVNGVQVVYDVQGDAVRGPKRMGDHGLYRQSNFVLDVEGGEVLTEISVKAGAIVDSVRVRTNKGREKTWGGAGGHLQRTWHVPTGSSFLGFHGGVGGHVHSLGVTLAERGGQAAGASGESSLALDPVVKTNLYAADRVARACAQFLAFNAPXXXXXXXXXXXXXXXXXXXXXXXXXXALEEVVTALETMRKYADNLLASPLDPKVSRIRLANGFFDRKIGRLAGGGGVVRAMGFELADEGGRMHYVFRRQGGGGGLG 1314
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BLAST of mRNA_Ecto-sp13_S_contig16138.4475.1 vs. uniprot
Match: A0A836CB71_9STRA (Putative peptide n-glycanase n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A836CB71_9STRA)

HSP 1 Score: 158 bits (400), Expect = 6.090e-38
Identity = 127/344 (36.92%), Postives = 163/344 (47.38%), Query Frame = 1 
Query:  358 VDCPGWTTVL---QVPGDMAGVASADDAGKEEVSVVPPVGWVVTTVHPAVGGMHEDTEEFDDGPVGCPAF-VMGGSPVALTADGS-EPPFLPAGRVREIVGWSGDA-VNGVQVVYDVQGDAVRGPKRMGDHGLYRQSNFVLDVEGGEVLTEISVKAGAIVDSLRVRTNKGREKTWGGAGGHLQ-RTWHVPTGSSFLGFHGGVGGHVHSLGV-TLAERGGQAAGASGETSLALDPVVKTNLYAADRVARACAQLLAFNAPXXXXXXXXXXXXXXXXXXXXXXXXXXALEEVVTALETMRKYADNLLASPLDPKVSRIRLANGFFDRKIGRLAGGGGVVRAMGFGL 1365
            V  PGW+T L    V  D A    A   G +         W V   H A G  H+DT  F D     PA    GG+  A+   GS     LP   V EI+GW+ DA VNGVQ+ Y +      G + +GDHG YRQS  +L+   GE +  + V+AG +VD + V T KGR  TWG A      R++ VP G +FLGF GGVGGH+HSLG+ T++E    AA A+ +  +         LY+AD   RA A L+  +A                           A      A ET  KYA N+L      K SRIR  N  F  KIG + GG  ++RA+GF L
Sbjct:  882 VHAPGWSTTLLPESVGVDGAAARGALPQGDDRGV------WRVVETHDATGSAHDDTIAFSDADTVFPAVGADGGAAAAVVLGGSIGGALLPLPHVVEILGWASDAHVNGVQLHYRIGTVRATGVRHLGDHGTYRQSAMMLN--DGEGVASVDVRAGLLVDQVTVTTTKGRSHTWGSALTESPLRSYSVPDGHTFLGFSGGVGGHLHSLGIITVSESETLAAAAAADADMPRFAAAVPLLYSADPARRAVAALMHLSADGGSSDSEA------------------AAAAARIAAETALKYAANVLRDAA--KFSRIRAGNAVFAAKIGSVRGGPLLLRALGFEL 1197
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BLAST of mRNA_Ecto-sp13_S_contig16138.4475.1 vs. uniprot
Match: A0A5J4DWW3_9BACT (Jacalin-type lectin domain-containing protein n=1 Tax=bacterium MnTg02 TaxID=2080452 RepID=A0A5J4DWW3_9BACT)

HSP 1 Score: 86.7 bits (213), Expect = 1.580e-16
Identity = 54/143 (37.76%), Postives = 80/143 (55.94%), Query Frame = 1 
Query:  550 PAFVMGGSPVALTADGSEPPFLPAGRVREIVGWSGDAVNGVQVVYDVQGDAVRGPKRMGDHGLYRQSNFVLDVEGGEVLTEISVKAGAIVDSLRVRTNKGREKTWGGAGGHLQRTWHVPTGSSFLGFHGGVGGHVHSLGVTLA 978
            P+  +GG P        + P+L   R+ EI  WSG A+N VQ+++D+ GDAV   K    HG +  S   L ++ GE LTEI  + G+ V+SL +RTN+G+ + +GG GG  +  +  P G    GF G  G  + S+GV +A
Sbjct:    8 PSGGVGGHPF------DDEPYLEHARLSEIRVWSGYAINAVQLLFDIDGDAVESRK----HGGHSGSIGTLRLDDGEYLTEIYGRYGSYVESLCLRTNRGQIRRFGGQGGSNEFVYLAPQGFQITGFWGCAGEMIDSIGVHIA 140
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BLAST of mRNA_Ecto-sp13_S_contig16138.4475.1 vs. uniprot
Match: UPI001D0592F6 (uncharacterized protein n=1 Tax=Fusarium poae TaxID=36050 RepID=UPI001D0592F6)

HSP 1 Score: 56.6 bits (135), Expect = 3.390e-5
Identity = 42/119 (35.29%), Postives = 63/119 (52.94%), Query Frame = 1 
Query:  625 RVREIVGWSGDAVNGVQVVY--DVQGDAVRGPKRMGDHGLYRQSNFVLDVEGGEVLTEISVKAGAIVDSLRVRTNKGREKTWGGAGGH--LQRTWHVPTGSSFL-GFHGGVGGHVHSLG 966
            RV+ +  W    +  +Q+ +  D  G     P R G     R  NFV+DV+ GE + E++V+ G  VD++ +RT++ RE + GG GG   +QR      G+ +L GF G  G  V SLG
Sbjct:  177 RVKRLDVWYSSVLQAIQLTWEDDSMGKWYGTPGRSGS----RHENFVMDVDKGESIKEMTVRGGQRVDAIWIRTSQNRELSAGGRGGTEVIQRL-----GNGYLTGFEGRAGWEVDSLG 286
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BLAST of mRNA_Ecto-sp13_S_contig16138.4475.1 vs. uniprot
Match: A0A0C3C1V6_HEBCY (Uncharacterized protein n=1 Tax=Hebeloma cylindrosporum h7 TaxID=686832 RepID=A0A0C3C1V6_HEBCY)

HSP 1 Score: 55.5 bits (132), Expect = 8.040e-5
Identity = 46/136 (33.82%), Postives = 64/136 (47.06%), Query Frame = 1 
Query:  559 VMGGSPVALTADGSEPPFLPAGRVREIVGWSGDAVNGVQVVYDVQGDAVRGPKRMGDHGLYRQSNFVLDVEGGEVLTEISVKAGAIVDSLRVRTNKGREKTWGGAGGHLQRTWHVPTGSSFLG-FHGGVGGHVHSL 963
            V GG   A   DGS    +   R+REI  W    +NG+Q+VYD QG            G+  Q +F L    GE +T++  + G  +D LR  TN GR+    G  G +   W  P+ ++ LG F G    +V SL
Sbjct:  155 VGGGGGAAF--DGSH--SVAGARLREIRLWIDFCINGIQLVYDFQGQTFSTGCWGRPWGVGTQKSFSL--RPGEFITKVEGRCGWYIDQLRFITNTGRQSDRFGGQGGIYFEWLPPSPTAGLGWFTGASASYVDSL 284
The following BLAST results are available for this feature:
BLAST of mRNA_Ecto-sp13_S_contig16138.4475.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 5
Match NameE-valueIdentityDescription
D7FKH8_ECTSI2.160e-28895.95Peptide n-glycanase, putative n=2 Tax=Ectocarpus T... [more]
A0A836CB71_9STRA6.090e-3836.92Putative peptide n-glycanase n=1 Tax=Tribonema min... [more]
A0A5J4DWW3_9BACT1.580e-1637.76Jacalin-type lectin domain-containing protein n=1 ... [more]
UPI001D0592F63.390e-535.29uncharacterized protein n=1 Tax=Fusarium poae TaxI... [more]
A0A0C3C1V6_HEBCY8.040e-533.82Uncharacterized protein n=1 Tax=Hebeloma cylindros... [more]
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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
Ecto-sp13_S_contig16138contigEcto-sp13_S_contig16138:1556..3981 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Ectocarpus species13 EcNAP12_S_4_19m OGS1.02022-07-08
Properties
Property NameValue
Taxonomic scopeEukaryota
Stop0
Start0
Seed ortholog score829.3
Seed ortholog evalue9e-238
Seed eggNOG ortholog2880.D7FKH8
Preferred namePNG1
Model size1430
KEGG koko:K01456,ko:K03671
KEGG Pathwayko04141,ko04621,ko05418,map04141,map04621,map05418
Hectar predicted targeting categoryother localisation
GOsGO:0000224,GO:0000302,GO:0001101,GO:0003674,GO:0003824,GO:0005488,GO:0005515,GO:0005575,GO:0005622,GO:0005623,GO:0005634,GO:0005737,GO:0005739,GO:0005829,GO:0006056,GO:0006058,GO:0006464,GO:0006508,GO:0006511,GO:0006515,GO:0006516,GO:0006517,GO:0006807,GO:0006950,GO:0006979,GO:0008150,GO:0008152,GO:0009056,GO:0009057,GO:0009100,GO:0009636,GO:0009719,GO:0009725,GO:0009751,GO:0009987,GO:0010033,GO:0010035,GO:0010188,GO:0010193,GO:0010243,GO:0010498,GO:0014070,GO:0016787,GO:0016810,GO:0016811,GO:0019538,GO:0019941,GO:0030163,GO:0030433,GO:0033554,GO:0034976,GO:0035966,GO:0035967,GO:0035977,GO:0036211,GO:0036503,GO:0042221,GO:0042493,GO:0043161,GO:0043167,GO:0043169,GO:0043170,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0043412,GO:0043632,GO:0044237,GO:0044238,GO:0044248,GO:0044257,GO:0044260,GO:0044265,GO:0044267,GO:0044424,GO:0044444,GO:0044464,GO:0046677,GO:0046872,GO:0050896,GO:0051603,GO:0051716,GO:0051787,GO:0051788,GO:0070887,GO:0071218,GO:0071310,GO:0071704,GO:0071712,GO:0097466,GO:1901135,GO:1901136,GO:1901564,GO:1901565,GO:1901575,GO:1901698,GO:1901700,GO:1904587
Exons3
EggNOG free text desc.Specifically deglycosylates the denatured form of N- linked glycoproteins in the cytoplasm and assists their proteasome-mediated degradation. Cleaves the beta-aspartyl- glucosamine (GlcNAc) of the glycan and the amide side chain of Asn, converting Asn to Asp. Prefers proteins containing high- mannose over those bearing complex type oligosaccharides. Can recognize misfolded proteins in the endoplasmic reticulum that are exported to the cytosol to be destroyed and deglycosylate them, while it has no activity toward native proteins. Deglycosylation is a prerequisite for subsequent proteasome-mediated degradation of some, but not all, misfolded glycoproteins
EggNOG OGsKOG0908@1,KOG0908@2759,KOG0909@1,KOG0909@2759
EC3.5.1.52
Cds size1428
COG Functional cat.T
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko01000,ko03110
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1681462851.5595021-CDS-Ecto-sp13_S_contig16138:1556..22041681462851.5595021-CDS-Ecto-sp13_S_contig16138:1556..2204Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig16138 1557..2204 -
1681462851.572324-CDS-Ecto-sp13_S_contig16138:2552..27871681462851.572324-CDS-Ecto-sp13_S_contig16138:2552..2787Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig16138 2553..2787 -
1681462851.5822105-CDS-Ecto-sp13_S_contig16138:3436..39811681462851.5822105-CDS-Ecto-sp13_S_contig16138:3436..3981Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig16138 3437..3981 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_Ecto-sp13_S_contig16138.4475.1prot_Ecto-sp13_S_contig16138.4475.1Ectocarpus species13 EcNAP12_S_4_19mpolypeptideEcto-sp13_S_contig16138 1557..3981 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_Ecto-sp13_S_contig16138.4475.1

>prot_Ecto-sp13_S_contig16138.4475.1 ID=prot_Ecto-sp13_S_contig16138.4475.1|Name=mRNA_Ecto-sp13_S_contig16138.4475.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=polypeptide|length=476bp
KPPDDESTPTAIAVVGLKKGPTGARRWVRRQHRGEDGGGGRCALYAEILL
PPPASGRAAAVQVELQDKLSLCPLRSLPAEGDAVAATTTPSAAPVSAPAR
GTCRRGGEPTVSVGPAVDLVDCPGWTTVLQVPGDMAGVASADDAGKEEVS
VVPPVGWVVTTVHPAVGGMHEDTEEFDDGPVGCPAFVMGGSPVALTADGS
EPPFLPAGRVREIVGWSGDAVNGVQVVYDVQGDAVRGPKRMGDHGLYRQS
NFVLDVEGGEVLTEISVKAGAIVDSLRVRTNKGREKTWGGAGGHLQRTWH
VPTGSSFLGFHGGVGGHVHSLGVTLAERGGQAAGASGETSLALDPVVKTN
LYAADRVARACAQLLAFNAPPPGGGKAGRSSGASSPARRTPPPPPPALEE
VVTALETMRKYADNLLASPLDPKVSRIRLANGFFDRKIGRLAGGGGVVRA
MGFGLADEGGRMHYVFRRQGAGGGLG
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mRNA from alignment at Ecto-sp13_S_contig16138:1556..3981-

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_Ecto-sp13_S_contig16138.4475.1 ID=mRNA_Ecto-sp13_S_contig16138.4475.1|Name=mRNA_Ecto-sp13_S_contig16138.4475.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=mRNA|length=2426bp|location=Sequence derived from alignment at Ecto-sp13_S_contig16138:1556..3981- (Ectocarpus species13 EcNAP12_S_4_19m)
AAGCCTCCGGACGACGAGAGCACGCCAACGGCGATCGCGGTCGTGGGCTT
GAAGAAGGGACCGACCGGTGCCCGGAGATGGGTTCGCCGTCAGCACCGGG
GCGAAGACGGTGGCGGCGGGCGCTGCGCCCTGTACGCGGAAATCCTGCTG
CCCCCCCCCGCCTCGGGCCGCGCCGCCGCCGTCCAGGTCGAACTGCAGGA
CAAGCTTTCCCTGTGCCCGCTGAGGTCGCTGCCGGCCGAAGGCGATGCGG
TGGCGGCGACGACTACGCCGTCTGCGGCGCCGGTTTCTGCGCCCGCCCGC
GGAACCTGCCGCCGCGGGGGGGAACCGACGGTGTCGGTGGGGCCGGCGGT
AGACCTGGTTGACTGCCCGGGCTGGACGACCGTGCTCCAAGTTCCCGGGG
ACATGGCGGGGGTTGCGTCGGCCGACGACGCGGGGAAGGAGGAGGTATCG
GTGGTGCCCCCGGTTGGCTGGGTGGTGACGACGGTTCACCCTGCGGTCGG
CGGGATGCACGAGGACACGGAGGAGTTCGATGACGGGCCGGTCGGGTGAG
GGTGTTTGTGTGTTTTTGGTCTTGTTGGGGGGTCTGGATGGGGCGTCATG
CAAGCAGCAGCAGCAGCTGCAGCAGCAAGAGTGTGTGTTGTGACGTCAAT
GACACATGTTTGCAGGACCGCGGGTGACCCGGTTGAACCCGTGGCTGGGT
GTCATTTCCCCTATGTCTTTGATCTCTTTCGTGGGCTGGTTGCTCGCCAG
GCACCACGAAACGGGAATGTAGACATTTTTATGCAATGCTTTCTGGCGAT
GTTATAAATGTTTTCCCGCCGCATGCCTCTGCGGTATGCGGACGCGACCT
CTAGAAAGGTCCCCACCTCCCCACCGTCTATATCGAGGACGACTGATCCC
CCCTAATCACGTTGTCGTCGACTAAGAGATACCCAGTTCCGCTTACCCTT
AGAAACCTTTTCTCGTTTAGCCAACAAGACAGAAGCTCCTGAGGAGCTGG
GGGGGTATTGTGGAGGTGACCTTTGAGATGCCCTTAAAAATGGGCACCAC
ATGTCCTTAACGTCCGTTGTGGACGACTTGCTTTTTTCATCTTTAACGGC
CTTTTTGGACGACTTTTTTTTTTCCCATCGAAACCTTTTTTTTTATTCTT
TTTTTTCGGTTTTTTTTTCCCCCCCCCCCCCCCCCCCCTCGAAGGTGCCC
CGCGTTTGTCATGGGCGGCTCTCCCGTCGCGCTTACAGCGGACGGCAGTG
AGCCCCCGTTCCTGCCGGCCGGCCGCGTGCGAGAGATCGTCGGGTGGAGC
GGAGACGCCGTCAACGGCGTCCAGGTGGTCTACGACGTCCAAGGGGATGC
CGTCCGAGGGCCGAAGCGGATGGGCGACCACGGCCTGTACCGCCAATCGA
ATTTCGTGCTGGATGTCGAGGGCGGAGAGGTAAGGACAAGGGCGCAGCCA
CCCTGTGGATGGCCACTCCGCCAGCGGCCTGGCTGCGCGCACTTTTGAAG
TTTGTTCGGGGGGAACGAATCGAAGTATTTTCTGACGGGATTGCCACCCT
GCGGGTCTCGAGGAGTGTTCGTGCCGAACCAAAGGGTTGAAGATTGCCTT
GCCGAGTCACGTCGCTGGTGACCTTTGTGCTTTGCCGTTACAACCAGTGG
GGTGGGTTTGACAAGTAGATGGAAGCGGAAGGGTCGCTCTGTCATGACCC
GCCAGGTCGACCGATCTTTGTCTGCATGCTGCTTTTCTTGCTAATTTGGT
AACGTCATGACCGTCTCTTTTTTCCAGGTCTTGACCGAGATATCCGTCAA
GGCCGGGGCCATCGTCGACAGCTTGCGGGTCAGGACGAACAAGGGCCGGG
AAAAAACGTGGGGGGGCGCCGGCGGGCACCTGCAGCGAACCTGGCACGTA
CCGACGGGGAGCTCATTCCTGGGCTTTCACGGAGGCGTCGGGGGCCACGT
GCACAGCCTCGGGGTAACCCTTGCTGAGCGGGGGGGGCAGGCAGCGGGGG
CGAGCGGCGAGACTTCCCTAGCCCTTGACCCAGTCGTGAAGACCAACCTG
TACGCCGCCGACAGAGTGGCAAGGGCGTGCGCCCAGTTGCTGGCGTTCAA
TGCTCCGCCTCCCGGCGGCGGCAAGGCGGGGCGCTCCTCAGGCGCGTCGT
CGCCAGCACGGCGGACCCCTCCCCCCCCCCCGCCGGCTCTCGAGGAAGTC
GTGACCGCGCTCGAGACCATGCGCAAGTACGCCGACAATCTCCTGGCGTC
TCCCCTGGATCCGAAGGTTTCCCGCATCCGCCTTGCGAACGGATTTTTCG
ATCGTAAGATCGGCAGGCTAGCGGGAGGCGGAGGCGTCGTTCGCGCCATG
GGGTTCGGGCTCGCCGACGAAGGCGGCCGCATGCATTACGTCTTTCGGAG
GCAGGGGGCCGGGGGTGGGCTCGGGG
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Coding sequence (CDS) from alignment at Ecto-sp13_S_contig16138:1556..3981-

>mRNA_Ecto-sp13_S_contig16138.4475.1 ID=mRNA_Ecto-sp13_S_contig16138.4475.1|Name=mRNA_Ecto-sp13_S_contig16138.4475.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=CDS|length=1428bp|location=Sequence derived from alignment at Ecto-sp13_S_contig16138:1556..3981- (Ectocarpus species13 EcNAP12_S_4_19m)
AAGCCTCCGGACGACGAGAGCACGCCAACGGCGATCGCGGTCGTGGGCTT
GAAGAAGGGACCGACCGGTGCCCGGAGATGGGTTCGCCGTCAGCACCGGG
GCGAAGACGGTGGCGGCGGGCGCTGCGCCCTGTACGCGGAAATCCTGCTG
CCCCCCCCCGCCTCGGGCCGCGCCGCCGCCGTCCAGGTCGAACTGCAGGA
CAAGCTTTCCCTGTGCCCGCTGAGGTCGCTGCCGGCCGAAGGCGATGCGG
TGGCGGCGACGACTACGCCGTCTGCGGCGCCGGTTTCTGCGCCCGCCCGC
GGAACCTGCCGCCGCGGGGGGGAACCGACGGTGTCGGTGGGGCCGGCGGT
AGACCTGGTTGACTGCCCGGGCTGGACGACCGTGCTCCAAGTTCCCGGGG
ACATGGCGGGGGTTGCGTCGGCCGACGACGCGGGGAAGGAGGAGGTATCG
GTGGTGCCCCCGGTTGGCTGGGTGGTGACGACGGTTCACCCTGCGGTCGG
CGGGATGCACGAGGACACGGAGGAGTTCGATGACGGGCCGGTCGGGTGCC
CCGCGTTTGTCATGGGCGGCTCTCCCGTCGCGCTTACAGCGGACGGCAGT
GAGCCCCCGTTCCTGCCGGCCGGCCGCGTGCGAGAGATCGTCGGGTGGAG
CGGAGACGCCGTCAACGGCGTCCAGGTGGTCTACGACGTCCAAGGGGATG
CCGTCCGAGGGCCGAAGCGGATGGGCGACCACGGCCTGTACCGCCAATCG
AATTTCGTGCTGGATGTCGAGGGCGGAGAGGTCTTGACCGAGATATCCGT
CAAGGCCGGGGCCATCGTCGACAGCTTGCGGGTCAGGACGAACAAGGGCC
GGGAAAAAACGTGGGGGGGCGCCGGCGGGCACCTGCAGCGAACCTGGCAC
GTACCGACGGGGAGCTCATTCCTGGGCTTTCACGGAGGCGTCGGGGGCCA
CGTGCACAGCCTCGGGGTAACCCTTGCTGAGCGGGGGGGGCAGGCAGCGG
GGGCGAGCGGCGAGACTTCCCTAGCCCTTGACCCAGTCGTGAAGACCAAC
CTGTACGCCGCCGACAGAGTGGCAAGGGCGTGCGCCCAGTTGCTGGCGTT
CAATGCTCCGCCTCCCGGCGGCGGCAAGGCGGGGCGCTCCTCAGGCGCGT
CGTCGCCAGCACGGCGGACCCCTCCCCCCCCCCCGCCGGCTCTCGAGGAA
GTCGTGACCGCGCTCGAGACCATGCGCAAGTACGCCGACAATCTCCTGGC
GTCTCCCCTGGATCCGAAGGTTTCCCGCATCCGCCTTGCGAACGGATTTT
TCGATCGTAAGATCGGCAGGCTAGCGGGAGGCGGAGGCGTCGTTCGCGCC
ATGGGGTTCGGGCTCGCCGACGAAGGCGGCCGCATGCATTACGTCTTTCG
GAGGCAGGGGGCCGGGGGTGGGCTCGGG
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