mRNA_Ecto-sp13_S_contig16045.4423.1 (mRNA) Ectocarpus species13 EcNAP12_S_4_19m

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_Ecto-sp13_S_contig16045.4423.1
Unique NamemRNA_Ecto-sp13_S_contig16045.4423.1
TypemRNA
OrganismEctocarpus species13 EcNAP12_S_4_19m (Ectocarpus species13 EcNAP12_S_4_19m)
Homology
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BLAST of mRNA_Ecto-sp13_S_contig16045.4423.1 vs. uniprot
Match: D8LSR0_ECTSI (Uncharacterized protein n=2 Tax=Ectocarpus TaxID=2879 RepID=D8LSR0_ECTSI)

HSP 1 Score: 162 bits (410), Expect = 5.560e-44
Identity = 78/80 (97.50%), Postives = 79/80 (98.75%), Query Frame = 2 
Query:    2 QMVVGDETAHAARDSAPISILRPFDRGYITNWEPEFEVWRRMLGRNFLNADPRDSRLLATEAPFGPLVLQEAANEVVFEE 241
            QMVVGDETAHAARDSAPISILRPFDRGYITNWEPEFEVWRRMLGRNFLNADPRDSRLLATEAPFGPLVLQEAANEV+ EE
Sbjct:   40 QMVVGDETAHAARDSAPISILRPFDRGYITNWEPEFEVWRRMLGRNFLNADPRDSRLLATEAPFGPLVLQEAANEVLEEE 119
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BLAST of mRNA_Ecto-sp13_S_contig16045.4423.1 vs. uniprot
Match: A0A836CBT8_9STRA (Actin family n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A836CBT8_9STRA)

HSP 1 Score: 124 bits (310), Expect = 2.430e-30
Identity = 59/101 (58.42%), Postives = 70/101 (69.31%), Query Frame = 2 
Query:    5 MVVGDETAHAARDSAPISILRPFDRGYITNWEPEFEVWRRMLGRNFLNA-DPRDSRLLATEAPFGPLVLQEAANEVVFEEFGFAACYRCPSAALSCYHEQV 304
            ++ GDET  A RD + +   RP DRGY+ NWE E EVW+RM   + LN  +P+D  LL TE PF P++L E ANEVVFEE GF ACYRCP AALS Y EQV
Sbjct:   40 VLAGDETVDAVRDKSQLVYQRPHDRGYLINWENELEVWKRMFDASHLNVQNPQDHALLFTEPPFAPIMLAETANEVVFEELGFTACYRCPPAALSVYLEQV 140
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BLAST of mRNA_Ecto-sp13_S_contig16045.4423.1 vs. uniprot
Match: W7TWI5_9STRA (Actin-related protein 6 n=2 Tax=Monodopsidaceae TaxID=425072 RepID=W7TWI5_9STRA)

HSP 1 Score: 99.4 bits (246), Expect = 4.050e-21
Identity = 44/107 (41.12%), Postives = 69/107 (64.49%), Query Frame = 2 
Query:    2 QMVVGDETAHAARDSAPISILRPFDRGYITNWEPEFEVWRRMLGRNFLNADPRDSRLLATEAPFGPLVLQEAANEVVFEEFGFAACYRCPSAALSCYHEQVLEEERE 322
            Q ++G++   +  ++A +   RPFDRGY+T+WE E ++W R  G N    DP  + L+ TEAPF P  L   + +VVFEEFGFAAC + P+A+ S Y   ++E++++
Sbjct:   73 QWLIGEDM-DSVNNTANLQYTRPFDRGYLTDWEAEHQIWGRAFGANKAKVDPAQTHLVLTEAPFTPSPLATFSTQVVFEEFGFAACAKMPAASFSGYRYSLMEKQQQ 178
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BLAST of mRNA_Ecto-sp13_S_contig16045.4423.1 vs. uniprot
Match: A0A7C8M2E1_9PLEO (Actin family n=1 Tax=Massariosphaeria phaeospora TaxID=100035 RepID=A0A7C8M2E1_9PLEO)

HSP 1 Score: 80.1 bits (196), Expect = 2.720e-14
Identity = 38/88 (43.18%), Postives = 52/88 (59.09%), Query Frame = 2 
Query:   38 RDSAPISILRPFDRGYITNWEPEFEVWRRML--GRNFLNADPRDSRLLATEAPFGPLVLQEAANEVVFEEFGFAACYRCPSAALSCYH 295
            +D   ++  RP ++G+I NWE E  +W       R  L  DPR++ LL TE P  P  LQ+  +E+VFE+F FAA YRC  A L+ YH
Sbjct:   70 KDFGELAFRRPVEKGFIVNWEAEKTIWEHEFVEARAGLRCDPRETNLLLTEKPNCPRELQKNCDEIVFEQFEFAAYYRCVGATLNAYH 157
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BLAST of mRNA_Ecto-sp13_S_contig16045.4423.1 vs. uniprot
Match: A0A8H7VJD6_9FUNG (Uncharacterized protein n=1 Tax=Mucor circinatus TaxID=2054153 RepID=A0A8H7VJD6_9FUNG)

HSP 1 Score: 79.7 bits (195), Expect = 3.430e-14
Identity = 39/99 (39.39%), Postives = 60/99 (60.61%), Query Frame = 2 
Query:   11 VGDETAHAARDSAPISILRPFDRGYITNWEPEFEVWRRMLGRNFLNADPRDSRLLATEAPFGPLVLQEAANEVVFEEFGFAACYRCPSAALSCYHEQVL 307
            +GD+      D + +    PF+RG++TNW+ E  VW R+   N L  DP+DSRLL TE  F    +QEA ++++FEE+ FA+CYR  +  L  +++  L
Sbjct:   43 IGDQIDQCT-DFSSLYYRLPFERGFLTNWDVERPVWNRLFS-NVLQIDPKDSRLLITEPCFNLKNIQEAYDQIIFEEYEFASCYRTIAPELCTFNDLAL 139
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BLAST of mRNA_Ecto-sp13_S_contig16045.4423.1 vs. uniprot
Match: L8GW12_ACACA (Actinrelated protein Arp6, putative n=1 Tax=Acanthamoeba castellanii str. Neff TaxID=1257118 RepID=L8GW12_ACACA)

HSP 1 Score: 79.7 bits (195), Expect = 3.540e-14
Identity = 36/92 (39.13%), Postives = 61/92 (66.30%), Query Frame = 2 
Query:   38 RDSAPISILRPFDRGYITNWEPEFEVWRRMLGRNFLNADPRDSRLLATEAPFGPLVLQEAANEVVFEEFGFAACYRC-PSAALSCYHEQVLE 310
            RD + + + RPF++GY+ ++E + E+W R  G+++L   P+D  L+ T+  F PL LQEA  E+VFEE+GF + + C P+ A+  Y+++  E
Sbjct:   49 RDISGLYLRRPFEKGYLVDFELQKEIWDRAFGKHYLKIQPKDHDLVLTQPLFTPLALQEATLELVFEEYGFKSLFCCSPATAVMHYYQKQPE 140
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BLAST of mRNA_Ecto-sp13_S_contig16045.4423.1 vs. uniprot
Match: A0A6A6SC62_9PLEO (Actin/actin-like protein n=1 Tax=Massarina eburnea CBS 473.64 TaxID=1395130 RepID=A0A6A6SC62_9PLEO)

HSP 1 Score: 79.0 bits (193), Expect = 6.870e-14
Identity = 41/97 (42.27%), Postives = 54/97 (55.67%), Query Frame = 2 
Query:   11 VGDETAHAARDSAPISILRPFDRGYITNWEPEFEVWRR--MLGRNFLNADPRDSRLLATEAPFGPLVLQEAANEVVFEEFGFAACYRCPSAALSCYH 295
            V  E  H  +D   ++  RP ++G+I NWE E  +W    M     L  DP D+ LL TE P  P  LQ+  +E+VFE+F FAA YRC   AL+ YH
Sbjct:   62 VASELEHC-KDFGELAFRRPVEKGFIVNWEAEKAIWEHEFMGAGEGLRCDPSDTNLLLTEKPNCPKELQKNCDEIVFEQFEFAAYYRCIGPALNAYH 157
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BLAST of mRNA_Ecto-sp13_S_contig16045.4423.1 vs. uniprot
Match: A0A6G1KYN0_9PEZI (Actin-related protein, ARP6 class n=1 Tax=Teratosphaeria nubilosa TaxID=161662 RepID=A0A6G1KYN0_9PEZI)

HSP 1 Score: 79.0 bits (193), Expect = 7.280e-14
Identity = 40/107 (37.38%), Postives = 54/107 (50.47%), Query Frame = 2 
Query:   11 VGDETAHAARDSAPISILRPFDRGYITNWEPEFEVWRRMLGRNF-------------LNADPRDSRLLATEAPFGPLVLQEAANEVVFEEFGFAACYRCPSAALSCY 292
            +G E +    D   +   RP ++GYI NWE E  +W +     +             L  DP ++ LL TEAP  P  LQ   +E+VFEEFGF A +RC + ALS Y
Sbjct:   71 IGAELSEECEDYGELKYRRPVEKGYIVNWESEKAIWEKTFLSGYGQKSADRGGVGDGLVCDPAETNLLLTEAPNAPAALQRNCDEMVFEEFGFGAYFRCLAPALSAY 177
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BLAST of mRNA_Ecto-sp13_S_contig16045.4423.1 vs. uniprot
Match: A0A163EZV0_DIDRA (Uncharacterized protein n=2 Tax=Ascochyta TaxID=5453 RepID=A0A163EZV0_DIDRA)

HSP 1 Score: 78.6 bits (192), Expect = 9.380e-14
Identity = 36/90 (40.00%), Postives = 54/90 (60.00%), Query Frame = 2 
Query:   32 AARDSAPISILRPFDRGYITNWEPEFEVWRR--MLGRNFLNADPRDSRLLATEAPFGPLVLQEAANEVVFEEFGFAACYRCPSAALSCYH 295
            + +D   ++  RP ++G+I NWE E  +W    M  R  L  +P+++ L+ TE P  P  LQ+  +E+VFE+F FAA YRC  A L+ YH
Sbjct:   68 SCKDFGELAFRRPVEKGFIVNWEAEKAIWEHEFMGAREGLRCEPKETNLVLTEKPNCPRELQKNCDEIVFEQFEFAAYYRCVGATLAAYH 157
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BLAST of mRNA_Ecto-sp13_S_contig16045.4423.1 vs. uniprot
Match: A0A068RRA9_9FUNG (Arp6 actin-related protein 6 homolog n=2 Tax=Lichtheimia TaxID=688353 RepID=A0A068RRA9_9FUNG)

HSP 1 Score: 77.8 bits (190), Expect = 1.590e-13
Identity = 37/96 (38.54%), Postives = 58/96 (60.42%), Query Frame = 2 
Query:   11 VGDETAHAARDSAPISILRPFDRGYITNWEPEFEVWRRMLGRNFLNADPRDSRLLATEAPFGPLVLQEAANEVVFEEFGFAACYRCPSAALSCYHE 298
            +GD+      D + +    PF+RGY+TNW  E  +W R+ G + LN DP+DS +L TE  F    +QE  ++V+FE++ FA+CYR  +  L  Y++
Sbjct:   42 IGDQIEQCT-DFSSLYYRLPFERGYLTNWGVERPIWNRLFG-SVLNIDPKDSGILITEPCFNLSNIQEVYDQVIFEDYEFASCYRTTAPQLCIYND 135
The following BLAST results are available for this feature:
BLAST of mRNA_Ecto-sp13_S_contig16045.4423.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
D8LSR0_ECTSI5.560e-4497.50Uncharacterized protein n=2 Tax=Ectocarpus TaxID=2... [more]
A0A836CBT8_9STRA2.430e-3058.42Actin family n=1 Tax=Tribonema minus TaxID=303371 ... [more]
W7TWI5_9STRA4.050e-2141.12Actin-related protein 6 n=2 Tax=Monodopsidaceae Ta... [more]
A0A7C8M2E1_9PLEO2.720e-1443.18Actin family n=1 Tax=Massariosphaeria phaeospora T... [more]
A0A8H7VJD6_9FUNG3.430e-1439.39Uncharacterized protein n=1 Tax=Mucor circinatus T... [more]
L8GW12_ACACA3.540e-1439.13Actinrelated protein Arp6, putative n=1 Tax=Acanth... [more]
A0A6A6SC62_9PLEO6.870e-1442.27Actin/actin-like protein n=1 Tax=Massarina eburnea... [more]
A0A6G1KYN0_9PEZI7.280e-1437.38Actin-related protein, ARP6 class n=1 Tax=Teratosp... [more]
A0A163EZV0_DIDRA9.380e-1440.00Uncharacterized protein n=2 Tax=Ascochyta TaxID=54... [more]
A0A068RRA9_9FUNG1.590e-1338.54Arp6 actin-related protein 6 homolog n=2 Tax=Licht... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
Ecto-sp13_S_contig16045contigEcto-sp13_S_contig16045:1620..3651 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Ectocarpus species13 EcNAP12_S_4_19m OGS1.02022-07-08
Properties
Property NameValue
Taxonomic scopeEukaryota
Stop1
Start1
Seed ortholog score160.2
Seed ortholog evalue5.4e-37
Seed eggNOG ortholog2880.D8LSR0
Preferred nameACTR6
Model size553
KEGG koko:K11662
Hectar predicted targeting categoryother localisation
GOsGO:0000228,GO:0000781,GO:0000784,GO:0000785,GO:0000790,GO:0000812,GO:0003674,GO:0003682,GO:0005488,GO:0005575,GO:0005622,GO:0005623,GO:0005634,GO:0005694,GO:0005737,GO:0006325,GO:0006338,GO:0006342,GO:0006348,GO:0006355,GO:0006996,GO:0008150,GO:0009889,GO:0009890,GO:0009892,GO:0009987,GO:0010468,GO:0010556,GO:0010558,GO:0010605,GO:0010629,GO:0016043,GO:0016458,GO:0019219,GO:0019222,GO:0031323,GO:0031324,GO:0031326,GO:0031327,GO:0031491,GO:0031974,GO:0031981,GO:0032991,GO:0034399,GO:0034728,GO:0040029,GO:0043044,GO:0043226,GO:0043227,GO:0043228,GO:0043229,GO:0043231,GO:0043232,GO:0043233,GO:0043486,GO:0043933,GO:0044422,GO:0044424,GO:0044427,GO:0044428,GO:0044446,GO:0044454,GO:0044464,GO:0044877,GO:0045814,GO:0045892,GO:0045934,GO:0048519,GO:0048523,GO:0050789,GO:0050794,GO:0051171,GO:0051172,GO:0051252,GO:0051253,GO:0051276,GO:0060255,GO:0065007,GO:0070013,GO:0070603,GO:0070828,GO:0071824,GO:0071840,GO:0080090,GO:0097346,GO:0098687,GO:1902494,GO:1902679,GO:1903506,GO:1903507,GO:1904949,GO:2000112,GO:2000113,GO:2001141
Exons3
EggNOG free text desc.chromatin remodeling
EggNOG OGsKOG0680@1,KOG0680@2759
Cds size327
COG Functional cat.S
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko03036,ko04812
Relationships

The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1681462847.4411073-UTR-Ecto-sp13_S_contig16045:1619..18411681462847.4411073-UTR-Ecto-sp13_S_contig16045:1619..1841Ectocarpus species13 EcNAP12_S_4_19mUTREcto-sp13_S_contig16045 1620..1841 -
1681462847.4822712-UTR-Ecto-sp13_S_contig16045:3647..36511681462847.4822712-UTR-Ecto-sp13_S_contig16045:3647..3651Ectocarpus species13 EcNAP12_S_4_19mUTREcto-sp13_S_contig16045 3648..3651 -


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1681462847.4552863-CDS-Ecto-sp13_S_contig16045:1841..19461681462847.4552863-CDS-Ecto-sp13_S_contig16045:1841..1946Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig16045 1842..1946 -
1681462847.4642146-CDS-Ecto-sp13_S_contig16045:2797..29111681462847.4642146-CDS-Ecto-sp13_S_contig16045:2797..2911Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig16045 2798..2911 -
1681462847.4727702-CDS-Ecto-sp13_S_contig16045:3539..36471681462847.4727702-CDS-Ecto-sp13_S_contig16045:3539..3647Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig16045 3540..3647 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_Ecto-sp13_S_contig16045.4423.1prot_Ecto-sp13_S_contig16045.4423.1Ectocarpus species13 EcNAP12_S_4_19mpolypeptideEcto-sp13_S_contig16045 1842..3647 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_Ecto-sp13_S_contig16045.4423.1

>prot_Ecto-sp13_S_contig16045.4423.1 ID=prot_Ecto-sp13_S_contig16045.4423.1|Name=mRNA_Ecto-sp13_S_contig16045.4423.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=polypeptide|length=109bp
MVVGDETAHAARDSAPISILRPFDRGYITNWEPEFEVWRRMLGRNFLNAD
PRDSRLLATEAPFGPLVLQEAANEVVFEEFGFAACYRCPSAALSCYHEQV
LEEERESM*
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mRNA from alignment at Ecto-sp13_S_contig16045:1620..3651-

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_Ecto-sp13_S_contig16045.4423.1 ID=mRNA_Ecto-sp13_S_contig16045.4423.1|Name=mRNA_Ecto-sp13_S_contig16045.4423.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=mRNA|length=2032bp|location=Sequence derived from alignment at Ecto-sp13_S_contig16045:1620..3651- (Ectocarpus species13 EcNAP12_S_4_19m)
TCAGATGGTGGTCGGAGACGAGACGGCGCACGCGGCGAGAGATTCGGCGC
CGATATCGATCCTGAGACCGTTCGACCGCGGGTACATCACCAACTGGGAG
CCCGAGTTCGAGGTGTGCGTTCGACCAAAAGATAATCTGTTGCTTTTGCC
GAGAGGGAAGAATGTGATCGAACCACTGGGATAAGGAGGGTCTTGACGCG
TGTGCGGAATGTTGACAGAAGGAAGAGCGTTTAAGGCGTGCCTTTATGGA
CCAAGTGGGTGCCGGGACAAGCCCATGACAAGGTCAAAATGTGGACTGGT
AACGTTGCTTGTGTCAAGCCCCCTGATTTCGCCAGGGTGAGTGGGCGGCA
CACACAACCTGTAGATATCGGGAAACCTGTGTAGTTTGCAGTGGCGTTTT
GATCTGTGGTGGCGGCAGCTGCTGCTGCTGCTGCTCCTGTTTTCTGCTTT
GCCCTCTTGTTGCACAGGGTTCCTTAACTCCTGCTGCTGCTGCTGTTATC
TTCTTTGTGCTGTTGGTTGGAAGCCATTAACAGGTTTATCTTGTAGCCCG
TGACCCCGGTCTTGTTTTTTCTTTCGTTCGGTCAGAAGCAGTCCACAGAT
ATGGCAAAACCTCCGGGATGGGGAATCACGCCGGTGTTTTTATGTGAAAA
CACCTCCACCACCACCAGCAGCCACAACAGCAAACGCAACGACAACCATA
CGTACCAACAACATCATTCAATTGCCTACAACCTCGTCAGGTGTGGCGAC
GCATGCTTGGACGAAATTTCCTCAACGCGGACCCTCGCGACAGCCGCCTC
CTTGCCACCGAGGCCCCCTTCGGTCCCCTCGTTCTCCAGGAAGCGGCCAA
CGAGGTGCGACCTAGACTTCGCGGCTTTAACGAGCAGAGGGCGGGAGGAG
TTAATATAGAGATGGAAAGGATCTTAGAACAGTCACATGCAACCACAGCA
AGACGATCCGTTCGAGATATAACCACGGTCGCAACGATAATTGTTGCAAA
AAGGCAGGGGAAAAGGACGCGCTTGCATCTAGTAAGAAGATTGGTGGCGC
CTTTTTTTTTTCATGTTTGCGTTGTTTACAGAGAGAAGACGTTGTATCGT
GTATGGAAGAAGTTGTATTCGCGCTAAGACACAGCAGAGGTGTGTGTGCA
TCCAGCTACTGTGCTGGTGGCTGTTCGTTGGATCGGACGCCGTTACGGCA
GCAGAAATGAAATATCCAACTTCGGTCATGGCATCGGAGTGTTGAGAAAT
ATGAAGACTGGCAGTACGGGCAATGCGGTGTTCTGCATCGGGTGTGGCAC
ACTGTGTCACGCAATCCTTCCTCTCACCTTTGAGTCCCTTGTGTTGGTCG
ACGTTCGAGCTGTTACTGCTTTCACCCCGTTTCATATTTTGCATTCACGG
GTGGCATCCCCTGTGAGGGCGGTACCCTTGTGATATCTGTATACTTTTTA
ACTTTTTCCACCATTTTAGAAGTTGAGCGACTACGCTGCTCACGCCCGCT
TGGCATCCGAGCAAGACCAACACGTGAATAGCGGGTGTCCTTGATTGTGG
TCTTGACTATCGCACCCCTCGTCGACGGCCTGTTTGAAACCATGATCGAC
ACTATTTACCGTGTTTCGGGACTCCGCCGGGCGTGGCTGGCCTGTGCTTA
ACTGGCGATCTGTCACCTGCTGCTGCTGCTGCTGCTGCTGCTGCTGCTGC
TGCAGGTAGTGTTTGAAGAGTTTGGGTTCGCGGCGTGCTACCGGTGTCCC
TCAGCGGCACTGAGCTGCTACCACGAGCAGGTACTGGAGGAGGAGAGGGA
GAGCATGTGAGTTGCTGCTGCTGATCCTGCTAAAACTGCTTCCGGGGCCG
GGAGGTTGTTGTTTTGGGTGACTCGCCGTAGCAGTGCACCAAGTAGGTTT
CCGTTCTCTCTCTCTATGTATATGTTCCTATCTCTTTCTCTAACTATCTT
TCTCCCTCTTCTCCCTCTTCTCCCTCTTCTCCCTCTGTCTCTCTCTCTCG
TGGCCCTCTCGTCCCTGCAGCAGCGCCCGAAA
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Coding sequence (CDS) from alignment at Ecto-sp13_S_contig16045:1620..3651-

>mRNA_Ecto-sp13_S_contig16045.4423.1 ID=mRNA_Ecto-sp13_S_contig16045.4423.1|Name=mRNA_Ecto-sp13_S_contig16045.4423.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=CDS|length=327bp|location=Sequence derived from alignment at Ecto-sp13_S_contig16045:1620..3651- (Ectocarpus species13 EcNAP12_S_4_19m)
ATGGTGGTCGGAGACGAGACGGCGCACGCGGCGAGAGATTCGGCGCCGAT
ATCGATCCTGAGACCGTTCGACCGCGGGTACATCACCAACTGGGAGCCCG
AGTTCGAGGTGTGGCGACGCATGCTTGGACGAAATTTCCTCAACGCGGAC
CCTCGCGACAGCCGCCTCCTTGCCACCGAGGCCCCCTTCGGTCCCCTCGT
TCTCCAGGAAGCGGCCAACGAGGTAGTGTTTGAAGAGTTTGGGTTCGCGG
CGTGCTACCGGTGTCCCTCAGCGGCACTGAGCTGCTACCACGAGCAGGTA
CTGGAGGAGGAGAGGGAGAGCATGTGA
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