mRNA_Ecto-sp13_S_contig1595.4366.1 (mRNA) Ectocarpus species13 EcNAP12_S_4_19m

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_Ecto-sp13_S_contig1595.4366.1
Unique NamemRNA_Ecto-sp13_S_contig1595.4366.1
TypemRNA
OrganismEctocarpus species13 EcNAP12_S_4_19m (Ectocarpus species13 EcNAP12_S_4_19m)
Homology
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BLAST of mRNA_Ecto-sp13_S_contig1595.4366.1 vs. uniprot
Match: A0A6H5JKS8_9PHAE (RING-type domain-containing protein n=2 Tax=Ectocarpus TaxID=2879 RepID=A0A6H5JKS8_9PHAE)

HSP 1 Score: 191 bits (485), Expect = 4.170e-57
Identity = 97/116 (83.62%), Postives = 97/116 (83.62%), Query Frame = 1 
Query:    1 INWGSVRRAVSGGAGRLRREARALGIFGGXXXXXXXXXXXX--ADAAPAAEAA-AEVVLTACAECGVNPAKTPYVTGCGHVFCYYCARVACTVDPRYACPRCGEYFATSRRWSQPR 339
            INWG VRRAVSGGAGRLRREARA GIFGG              AD APAAEAA AEVVLTACAECG NPAKTPYVTGCGHVFCYYCARVACTVDPRYACPRCGEYFATSRRWSQPR
Sbjct:  318 INWGFVRRAVSGGAGRLRREARAFGIFGGSSGGAGRVSARGLGADTAPAAEAAVAEVVLTACAECGANPAKTPYVTGCGHVFCYYCARVACTVDPRYACPRCGEYFATSRRWSQPR 433
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BLAST of mRNA_Ecto-sp13_S_contig1595.4366.1 vs. uniprot
Match: W7TYI4_9STRA (Peroxin-2 n=1 Tax=Nannochloropsis gaditana TaxID=72520 RepID=W7TYI4_9STRA)

HSP 1 Score: 83.6 bits (205), Expect = 1.710e-16
Identity = 46/109 (42.20%), Postives = 57/109 (52.29%), Query Frame = 1 
Query:    1 INWGSVRRAVSGGAGRLRREARALGIFGGXXXXXXXXXXXXADAAPAAEAAAEVVLTACAECGVNPAKTPYVTGCGHVFCYYCARVACTVDPRYACPRCGEYFATSRRW 327
            ++W  +RR VSG    L R+ RA     G  XX          A    E         C  CG   A+TPYVT CGH++CYYC + AC  D  ++CPRCG +FATSRRW
Sbjct:  336 VDWDGLRRQVSG----LIRQRRAGAWAAGAGXXGGSEFRGAGGADGDME---------CVVCGEESAQTPYVTDCGHIYCYYCLKTACLQDDSFSCPRCGTFFATSRRW 431
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BLAST of mRNA_Ecto-sp13_S_contig1595.4366.1 vs. uniprot
Match: A0A1W0A7A5_9STRA (Peroxin-2 n=1 Tax=Thraustotheca clavata TaxID=74557 RepID=A0A1W0A7A5_9STRA)

HSP 1 Score: 78.2 bits (191), Expect = 8.050e-15
Identity = 31/53 (58.49%), Postives = 37/53 (69.81%), Query Frame = 1 
Query:  166 LTACAECGVNPAKTPYVTGCGHVFCYYCARVACTVDPRYACPRCGEYFATSRR 324
            LTACA C V PAKTPY+T CGH +CY+C + A  +DP Y C  CGE F +S R
Sbjct:  260 LTACALCSVTPAKTPYITSCGHQYCYFCLQTAVAMDPNYTCAMCGEVFESSER 312
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BLAST of mRNA_Ecto-sp13_S_contig1595.4366.1 vs. uniprot
Match: A0A1V9ZKW2_9STRA (Peroxin-2 n=1 Tax=Achlya hypogyna TaxID=1202772 RepID=A0A1V9ZKW2_9STRA)

HSP 1 Score: 74.3 bits (181), Expect = 2.090e-13
Identity = 28/53 (52.83%), Postives = 36/53 (67.92%), Query Frame = 1 
Query:  166 LTACAECGVNPAKTPYVTGCGHVFCYYCARVACTVDPRYACPRCGEYFATSRR 324
            L  C  CG++PAKTPY+T CGH +CY+C + A   DP YAC  CG+ F +S R
Sbjct:  260 LAPCTICGISPAKTPYITSCGHQYCYFCLQTAVATDPTYACATCGDTFESSER 312
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BLAST of mRNA_Ecto-sp13_S_contig1595.4366.1 vs. uniprot
Match: UPI00064503DB (hypothetical protein n=1 Tax=Acytostelium subglobosum LB1 TaxID=1410327 RepID=UPI00064503DB)

HSP 1 Score: 70.9 bits (172), Expect = 4.650e-12
Identity = 29/68 (42.65%), Postives = 41/68 (60.29%), Query Frame = 1 
Query:  127 DAAPAAEAAAEVVLTACAECGVNPAKTPYVTGCGHVFCYYCARVACTVDPRYACPRCGEYFATSRRWS 330
            DAA A +  A +VL  C  C  +P   PY+T CGH+FCYYC +  C +DP + CPRC    +  +R++
Sbjct:  269 DAALAPQLLAPLVLEKCPICFNDPISVPYITDCGHLFCYYCIKTNCMIDPTFTCPRCNSIVSNMKRFT 336
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BLAST of mRNA_Ecto-sp13_S_contig1595.4366.1 vs. uniprot
Match: A0A067C940_SAPPC (Peroxin-2 n=2 Tax=Saprolegnia TaxID=4769 RepID=A0A067C940_SAPPC)

HSP 1 Score: 68.9 bits (167), Expect = 1.970e-11
Identity = 27/52 (51.92%), Postives = 32/52 (61.54%), Query Frame = 1 
Query:  175 CAECGVNPAKTPYVTGCGHVFCYYCARVACTVDPRYACPRCGEYFATSRRWS 330
            CA C   PAKTPY+T CGH +CYYC + A   DP + C  CG+ F  S R S
Sbjct:  263 CALCNATPAKTPYITTCGHQYCYYCLQTAVATDPTFVCATCGDVFDASERLS 314
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BLAST of mRNA_Ecto-sp13_S_contig1595.4366.1 vs. uniprot
Match: A0A485KU83_9STRA (Peroxin-2 n=1 Tax=Aphanomyces stellatus TaxID=120398 RepID=A0A485KU83_9STRA)

HSP 1 Score: 60.8 bits (146), Expect = 1.590e-8
Identity = 26/62 (41.94%), Postives = 36/62 (58.06%), Query Frame = 1 
Query:  154 AEVVLTACAECGVNPAKTPYVTGCGHVFCYYCARVACTVDPRYACPRCGEYFATSRRWSQPR 339
            A  +   C  C  +PAKTPY T CGHV+CYYC + A + +  + C  CG+ F +S+R    R
Sbjct:  256 ARALQNGCPICLTSPAKTPYKTSCGHVYCYYCLQSAVSSNADFVCVVCGDLFESSQRLGNDR 317
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BLAST of mRNA_Ecto-sp13_S_contig1595.4366.1 vs. uniprot
Match: A0A507DDU9_9FUNG (Peroxin-2 n=1 Tax=Synchytrium endobioticum TaxID=286115 RepID=A0A507DDU9_9FUNG)

HSP 1 Score: 60.8 bits (146), Expect = 1.730e-8
Identity = 22/37 (59.46%), Postives = 27/37 (72.97%), Query Frame = 1 
Query:  205 TPYVTGCGHVFCYYCARVACTVDPRYACPRCGEYFAT 315
            TPY +GCGHV+CYYC + A   DPR+ CPRCGE   +
Sbjct:  325 TPYKSGCGHVYCYYCVKSAMMADPRFPCPRCGEVITS 361
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BLAST of mRNA_Ecto-sp13_S_contig1595.4366.1 vs. uniprot
Match: W4GX78_9STRA (Peroxin-2 n=1 Tax=Aphanomyces astaci TaxID=112090 RepID=W4GX78_9STRA)

HSP 1 Score: 59.7 bits (143), Expect = 4.050e-8
Identity = 24/50 (48.00%), Postives = 31/50 (62.00%), Query Frame = 1 
Query:  175 CAECGVNPAKTPYVTGCGHVFCYYCARVACTVDPRYACPRCGEYFATSRR 324
            C  C   PAKTPY T CGH++CY+C + +    P +AC  CG+ F TS R
Sbjct:  265 CPICLAVPAKTPYQTSCGHIYCYFCLQSSVAAHPDFACVVCGDRFDTSHR 314
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BLAST of mRNA_Ecto-sp13_S_contig1595.4366.1 vs. uniprot
Match: L8HGF4_ACACA (Pex2 / Pex12 amino terminal regioncontaining protein n=1 Tax=Acanthamoeba castellanii str. Neff TaxID=1257118 RepID=L8HGF4_ACACA)

HSP 1 Score: 59.3 bits (142), Expect = 5.730e-8
Identity = 22/43 (51.16%), Postives = 28/43 (65.12%), Query Frame = 1 
Query:  175 CAECGVNPAKTPYVTGCGHVFCYYCARVACTVDPRYACPRCGE 303
            C  C  +PA +PY++ CGH+FCYYC + A  VD  YACP C  
Sbjct:  272 CGVCQADPAHSPYLSDCGHLFCYYCIKTAMMVDRSYACPSCNH 314
The following BLAST results are available for this feature:
BLAST of mRNA_Ecto-sp13_S_contig1595.4366.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 11
Match NameE-valueIdentityDescription
A0A6H5JKS8_9PHAE4.170e-5783.62RING-type domain-containing protein n=2 Tax=Ectoca... [more]
W7TYI4_9STRA1.710e-1642.20Peroxin-2 n=1 Tax=Nannochloropsis gaditana TaxID=7... [more]
A0A1W0A7A5_9STRA8.050e-1558.49Peroxin-2 n=1 Tax=Thraustotheca clavata TaxID=7455... [more]
A0A1V9ZKW2_9STRA2.090e-1352.83Peroxin-2 n=1 Tax=Achlya hypogyna TaxID=1202772 Re... [more]
UPI00064503DB4.650e-1242.65hypothetical protein n=1 Tax=Acytostelium subglobo... [more]
A0A067C940_SAPPC1.970e-1151.92Peroxin-2 n=2 Tax=Saprolegnia TaxID=4769 RepID=A0A... [more]
A0A485KU83_9STRA1.590e-841.94Peroxin-2 n=1 Tax=Aphanomyces stellatus TaxID=1203... [more]
A0A507DDU9_9FUNG1.730e-859.46Peroxin-2 n=1 Tax=Synchytrium endobioticum TaxID=2... [more]
W4GX78_9STRA4.050e-848.00Peroxin-2 n=1 Tax=Aphanomyces astaci TaxID=112090 ... [more]
L8HGF4_ACACA5.730e-851.16Pex2 / Pex12 amino terminal regioncontaining prote... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
Ecto-sp13_S_contig1595contigEcto-sp13_S_contig1595:14788..16480 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Ectocarpus species13 EcNAP12_S_4_19m OGS1.02022-07-08
Properties
Property NameValue
Taxonomic scopeEukaryota
Stop1
Start0
Seed ortholog score192.6
Seed ortholog evalue1e-46
Seed eggNOG ortholog2880.D8LL84
Preferred namePEX2
Model size342
KEGG koko:K06664
KEGG TC3.A.20.1
KEGG Pathwayko04146,map04146
Hectar predicted targeting categorymitochondrion
GOsGO:0000003,GO:0000038,GO:0000122,GO:0000151,GO:0000152,GO:0000209,GO:0000280,GO:0000428,GO:0000910,GO:0001764,GO:0003006,GO:0003674,GO:0003824,GO:0004842,GO:0005488,GO:0005575,GO:0005622,GO:0005623,GO:0005634,GO:0005654,GO:0005737,GO:0005777,GO:0005778,GO:0005779,GO:0005829,GO:0005911,GO:0006082,GO:0006355,GO:0006357,GO:0006464,GO:0006508,GO:0006511,GO:0006513,GO:0006605,GO:0006625,GO:0006629,GO:0006631,GO:0006635,GO:0006694,GO:0006699,GO:0006807,GO:0006810,GO:0006886,GO:0006928,GO:0006996,GO:0007031,GO:0007049,GO:0007112,GO:0007140,GO:0007275,GO:0007276,GO:0007281,GO:0007283,GO:0007285,GO:0007286,GO:0007399,GO:0008023,GO:0008104,GO:0008150,GO:0008152,GO:0008202,GO:0008206,GO:0008270,GO:0008285,GO:0008610,GO:0009056,GO:0009057,GO:0009058,GO:0009062,GO:0009314,GO:0009416,GO:0009506,GO:0009628,GO:0009639,GO:0009640,GO:0009791,GO:0009889,GO:0009890,GO:0009892,GO:0009987,GO:0010468,GO:0010498,GO:0010556,GO:0010558,GO:0010605,GO:0010629,GO:0015031,GO:0015833,GO:0015919,GO:0016020,GO:0016021,GO:0016042,GO:0016043,GO:0016049,GO:0016053,GO:0016054,GO:0016477,GO:0016558,GO:0016567,GO:0016591,GO:0016593,GO:0016740,GO:0017038,GO:0019216,GO:0019218,GO:0019219,GO:0019222,GO:0019395,GO:0019538,GO:0019752,GO:0019787,GO:0019941,GO:0019953,GO:0022008,GO:0022402,GO:0022412,GO:0022414,GO:0030054,GO:0030154,GO:0030163,GO:0030258,GO:0030880,GO:0031090,GO:0031224,GO:0031231,GO:0031300,GO:0031301,GO:0031323,GO:0031324,GO:0031326,GO:0031327,GO:0031399,GO:0031400,GO:0031647,GO:0031648,GO:0031903,GO:0031974,GO:0031981,GO:0032268,GO:0032269,GO:0032446,GO:0032501,GO:0032502,GO:0032504,GO:0032787,GO:0032991,GO:0033036,GO:0033206,GO:0033233,GO:0033234,GO:0033365,GO:0033768,GO:0034440,GO:0034613,GO:0036211,GO:0040007,GO:0040011,GO:0042127,GO:0042579,GO:0042592,GO:0042632,GO:0042886,GO:0043161,GO:0043167,GO:0043169,GO:0043170,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0043233,GO:0043412,GO:0043436,GO:0043574,GO:0043632,GO:0044237,GO:0044238,GO:0044242,GO:0044248,GO:0044249,GO:0044255,GO:0044257,GO:0044260,GO:0044265,GO:0044267,GO:0044281,GO:0044282,GO:0044283,GO:0044422,GO:0044424,GO:0044425,GO:0044428,GO:0044438,GO:0044439,GO:0044444,GO:0044446,GO:0044451,GO:0044464,GO:0044703,GO:0044743,GO:0045184,GO:0045540,GO:0045892,GO:0045934,GO:0046394,GO:0046395,GO:0046872,GO:0046890,GO:0046907,GO:0046914,GO:0048137,GO:0048145,GO:0048147,GO:0048232,GO:0048285,GO:0048468,GO:0048515,GO:0048519,GO:0048523,GO:0048588,GO:0048589,GO:0048609,GO:0048699,GO:0048731,GO:0048856,GO:0048869,GO:0048870,GO:0048878,GO:0050678,GO:0050680,GO:0050789,GO:0050794,GO:0050810,GO:0050896,GO:0051171,GO:0051172,GO:0051179,GO:0051234,GO:0051246,GO:0051248,GO:0051252,GO:0051253,GO:0051301,GO:0051321,GO:0051603,GO:0051641,GO:0051649,GO:0051674,GO:0051704,GO:0055029,GO:0055044,GO:0055085,GO:0055088,GO:0055092,GO:0055114,GO:0060255,GO:0061630,GO:0061640,GO:0061659,GO:0061695,GO:0062012,GO:0065002,GO:0065007,GO:0065008,GO:0070013,GO:0070647,GO:0070727,GO:0071702,GO:0071704,GO:0071705,GO:0071806,GO:0071840,GO:0072329,GO:0072330,GO:0072594,GO:0072662,GO:0072663,GO:0080090,GO:0090181,GO:0098588,GO:0098805,GO:0106118,GO:0140013,GO:0140096,GO:1901360,GO:1901362,GO:1901564,GO:1901565,GO:1901575,GO:1901576,GO:1901615,GO:1901617,GO:1902494,GO:1902679,GO:1902930,GO:1903046,GO:1903320,GO:1903321,GO:1903506,GO:1903507,GO:1990234,GO:1990351,GO:1990415,GO:1990429,GO:2000112,GO:2000113,GO:2001141
Exons2
EggNOG free text desc.protein import into peroxisome matrix
EggNOG OGsKOG2879@1,KOG2879@2759
Ec32 ortholog descriptionPex, N-terminal
Ec32 orthologEc-24_002570.2
Cds size342
COG Functional cat.S
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko04121
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1681462843.0316184-CDS-Ecto-sp13_S_contig1595:14787..149251681462843.0316184-CDS-Ecto-sp13_S_contig1595:14787..14925Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig1595 14788..14925 -
1681462843.0425959-CDS-Ecto-sp13_S_contig1595:16276..164801681462843.0425959-CDS-Ecto-sp13_S_contig1595:16276..16480Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig1595 16277..16480 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_Ecto-sp13_S_contig1595.4366.1prot_Ecto-sp13_S_contig1595.4366.1Ectocarpus species13 EcNAP12_S_4_19mpolypeptideEcto-sp13_S_contig1595 14788..16480 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_Ecto-sp13_S_contig1595.4366.1

>prot_Ecto-sp13_S_contig1595.4366.1 ID=prot_Ecto-sp13_S_contig1595.4366.1|Name=mRNA_Ecto-sp13_S_contig1595.4366.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=polypeptide|length=114bp
INWGSVRRAVSGGAGRLRREARALGIFGGSGSGRASARGLGADAAPAAEA
AAEVVLTACAECGVNPAKTPYVTGCGHVFCYYCARVACTVDPRYACPRCG
EYFATSRRWSQPR*
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mRNA from alignment at Ecto-sp13_S_contig1595:14788..16480-

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_Ecto-sp13_S_contig1595.4366.1 ID=mRNA_Ecto-sp13_S_contig1595.4366.1|Name=mRNA_Ecto-sp13_S_contig1595.4366.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=mRNA|length=1693bp|location=Sequence derived from alignment at Ecto-sp13_S_contig1595:14788..16480- (Ectocarpus species13 EcNAP12_S_4_19m)
ATAAACTGGGGCTCCGTGAGGAGAGCAGTGTCGGGAGGGGCGGGACGCTT
GAGAAGAGAAGCTCGCGCACTCGGCATATTTGGGGGCAGCGGCAGCGGCA
GGGCTTCGGCAAGAGGGCTGGGGGCTGATGCTGCTCCTGCTGCGGAAGCA
GCGGCGGAGGTGGTGCTCACGGCGTGCGCGGAATGTGGAGTCAACCCGGC
GAAGGTGACTATGTTCTAGGCTGTGTGTCCCCTCTCGCTCCCTTCGTTTG
GCCACCATTAAAAAAAGCAGCAATCCCTGCGGGTCTCCATCGTTAGTGTT
GTGTGGATTCATGCCGTAGTAGGTCAATCGTGCAATTTTCTGGGTAGTAC
CGCGGCAGCTCGTTCTAATCCCGAGCGAGCCTTCGCGCCTTGCAGCACGT
GAAGGACTAGTGTAATAGGTGGCGTTCTGTGCTCCCTTTTGCACGCATGC
ATCTCGAGAAGGGTAATGCTTCGACTACCTACCGTATACGACACAAGATA
TCCCAACCCCTGGACAAATTTTCACAGCTCGATTTCTCAACAGCACGAAG
AGTCCTAGCACCGAAAACGGCAGCCTTGAAGCCGTCTGGTCGAGACCTCT
GCGAAAACGATCGTTTCGTTCATGCACCCTCTTGGTCGTGGAGAAGTAGA
GCGTTGAAAATCGGTCCAGGAGGGGGGGGTGTTATCTTGTGTCGTACACG
GTATCCCCCGCCGAAGGTGTCTTCACGCGAGAGACGTGAGCAAGATGACG
TACATCACCACCCCCACAGAAGGGTCCACGACCCCCACAATGCACCACAC
AGCGCGATATCCTTCTTTCGGTAGGGTATAGTTGGCGGCGTGTGGTATAT
TGCGTCGTATACGGTGTAGCGTACCTGAGTAGGGCCAGTGTGAGGTCTAG
AACCGATTCCGTCTTTCGAAAGTTGAGCTACAACGAAGGGTCGTTCACAT
CAACGAGATCGTGGGGAGGTGGCTGTTGACGCGGGGCCATGTCAAGGGCC
ATCTTTCCTCCATCTCTTTGATTTGAACGATCCTTTCGTGTTGCTCAACT
TTCGAAAGATGCGCGTCAAAAGTCACCTCCCCTCCATCTCCTTGATTTCG
AAAGACGGAAGATCATCAAAACTGTTTTCGTCAGAATCAGGGTGATCGTT
GTTGGTAGTACTGTAGCTTGCTTTGTTTGACCCTATTCCGCAAAGTCGTG
TTAAGTTTGTCCACCTCACTTCCGGGCCGAAATTGGTTTGGTTTTGCAGC
TCGATGTTGGACCCACACGTGTGGCAACCTCTGCAGCCGAAGCGCTTGAT
CCATGCTGACGTGTACCAAGCGCCCAGATGTCTTGAACCCTCGTCGATCA
ATCGTCGCCTTTTCTCTGTGTCTCTCTCTGTCGCTCTCTTCCGTCCCATT
CGCTCACACGGAGCCCCCTCGCTTGAAGCCGTGCCTGTCATGTCTACCAC
CCCAGCTCGGTTGCCCCTGACCCATCGATACCCCACATTAACCTCCCCGT
CCCCTACTCCCGGCTGTCCATCGCTGCCCGGCGTTAACCTTGGCTTGCAT
CACAGACTCCTTACGTGACGGGGTGCGGCCACGTGTTCTGCTACTACTGC
GCGAGGGTGGCTTGCACGGTCGACCCCCGATACGCCTGTCCGCGCTGCGG
GGAGTACTTCGCCACATCCCGTCGATGGTCCCAGCCGCGGTGA
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Coding sequence (CDS) from alignment at Ecto-sp13_S_contig1595:14788..16480-

>mRNA_Ecto-sp13_S_contig1595.4366.1 ID=mRNA_Ecto-sp13_S_contig1595.4366.1|Name=mRNA_Ecto-sp13_S_contig1595.4366.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=CDS|length=342bp|location=Sequence derived from alignment at Ecto-sp13_S_contig1595:14788..16480- (Ectocarpus species13 EcNAP12_S_4_19m)
ATAAACTGGGGCTCCGTGAGGAGAGCAGTGTCGGGAGGGGCGGGACGCTT
GAGAAGAGAAGCTCGCGCACTCGGCATATTTGGGGGCAGCGGCAGCGGCA
GGGCTTCGGCAAGAGGGCTGGGGGCTGATGCTGCTCCTGCTGCGGAAGCA
GCGGCGGAGGTGGTGCTCACGGCGTGCGCGGAATGTGGAGTCAACCCGGC
GAAGACTCCTTACGTGACGGGGTGCGGCCACGTGTTCTGCTACTACTGCG
CGAGGGTGGCTTGCACGGTCGACCCCCGATACGCCTGTCCGCGCTGCGGG
GAGTACTTCGCCACATCCCGTCGATGGTCCCAGCCGCGGTGA
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