mRNA_Ecto-sp13_S_contig15723.4222.1 (mRNA) Ectocarpus species13 EcNAP12_S_4_19m

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_Ecto-sp13_S_contig15723.4222.1
Unique NamemRNA_Ecto-sp13_S_contig15723.4222.1
TypemRNA
OrganismEctocarpus species13 EcNAP12_S_4_19m (Ectocarpus species13 EcNAP12_S_4_19m)
Homology
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BLAST of mRNA_Ecto-sp13_S_contig15723.4222.1 vs. uniprot
Match: A0A6H5KTV2_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5KTV2_9PHAE)

HSP 1 Score: 235 bits (600), Expect = 2.570e-75
Identity = 115/117 (98.29%), Postives = 116/117 (99.15%), Query Frame = 1 
Query:    1 EGWQDALAEIVRSVTKTETKILSVCGKELSLAGFDDLAKEAYMKMDDFTNVMALYVKNQNWSEAVKLSEEHGRKYDDAVFLPYALWLRDKGRFDEALEAFQRAGRRDLSGAMTEQLM 351
            EGWQDALAEIVRSVTKTETKILSVCGKELSLAGFDDLAKEAYMKMDDFTNVMALYVKNQNWSEAVKLSEEHGRKYDDA+FLPYALWLRD GRFDEALEAFQRAGRRDLSGAMTEQLM
Sbjct:  231 EGWQDALAEIVRSVTKTETKILSVCGKELSLAGFDDLAKEAYMKMDDFTNVMALYVKNQNWSEAVKLSEEHGRKYDDAIFLPYALWLRDNGRFDEALEAFQRAGRRDLSGAMTEQLM 347
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BLAST of mRNA_Ecto-sp13_S_contig15723.4222.1 vs. uniprot
Match: D7FXW9_ECTSI (Intraflagellar transport protein 122 homolog n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FXW9_ECTSI)

HSP 1 Score: 234 bits (597), Expect = 3.550e-69
Identity = 115/117 (98.29%), Postives = 117/117 (100.00%), Query Frame = 1 
Query:    1 EGWQDALAEIVRSVTKTETKILSVCGKELSLAGFDDLAKEAYMKMDDFTNVMALYVKNQNWSEAVKLSEEHGRKYDDAVFLPYALWLRDKGRFDEALEAFQRAGRRDLSGAMTEQLM 351
            EGWQDALAEIVRSVTKTETKILSVCGKELSLAGFDDLAKEAYMKMDDFTNVMALYVK+QNWSEAVKLSEEHGRKYDDAVFLPYALWLRDKGRFDEALEAFQRAGRRDLSGAMTEQL+
Sbjct:  815 EGWQDALAEIVRSVTKTETKILSVCGKELSLAGFDDLAKEAYMKMDDFTNVMALYVKHQNWSEAVKLSEEHGRKYDDAVFLPYALWLRDKGRFDEALEAFQRAGRRDLSGAMTEQLI 931
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BLAST of mRNA_Ecto-sp13_S_contig15723.4222.1 vs. uniprot
Match: A0A812QAW2_SYMMI (Ift122 protein n=1 Tax=Symbiodinium microadriaticum TaxID=2951 RepID=A0A812QAW2_SYMMI)

HSP 1 Score: 107 bits (266), Expect = 9.490e-25
Identity = 51/115 (44.35%), Postives = 78/115 (67.83%), Query Frame = 1 
Query:    4 GWQDALAEIVRSVTKTETKILSVCGKELSLAGFDDLAKEAYMKMDDFTNVMALYVKNQNWSEAVKLSEEHGRKYDDAVFLPYALWLRDKGRFDEALEAFQRAGRRDLSGAMTEQL 348
            GWQ AL ++VR+       +L+ CG+ L+    D LA+EAY K+ D   +MAL+VK Q W EA KL++E+  K+D +VFLPYA WL  + R ++A++A+ +AGR+DL+  + E+L
Sbjct:   19 GWQLALIDVVRATPTDVRDVLAFCGEILTQHNEDALAREAYTKLGDVNKLMALFVKRQMWVEAAKLADENEGKFDSSVFLPYAEWLIIQDRCEDAIQAYNKAGRQDLARKILEEL 133
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BLAST of mRNA_Ecto-sp13_S_contig15723.4222.1 vs. uniprot
Match: A0A7S1UEE7_9STRA (Hypothetical protein (Fragment) n=1 Tax=Phaeomonas parva TaxID=124430 RepID=A0A7S1UEE7_9STRA)

HSP 1 Score: 104 bits (259), Expect = 3.740e-24
Identity = 50/116 (43.10%), Postives = 76/116 (65.52%), Query Frame = 1 
Query:    1 EGWQDALAEIVRSVTKTETKILSVCGKELSLAGFDDLAKEAYMKMDDFTNVMALYVKNQNWSEAVKLSEEHGRKYDDAVFLPYALWLRDKGRFDEALEAFQRAGRRDLSGAMTEQL 348
            EGW+ ALAE+  +  + ET+ L++CG+  +  G  D AK+ ++K+ D++ +M           AVKLSEEH  ++D ++FLPYA+WL  + RFD+AL A+ +AGR DLS  + EQL
Sbjct:  228 EGWEAALAEVAEACPRAETEALTICGRTFAEQGEFDHAKDVFVKLGDWSALMXXXXXXXXXXXAVKLSEEHEGEFDSSMFLPYAVWLALQDRFDDALRAYGKAGRPDLSFKLMEQL 343
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BLAST of mRNA_Ecto-sp13_S_contig15723.4222.1 vs. uniprot
Match: A0A835ZL39_9STRA (Intraflagellar transport protein 122 homolog n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835ZL39_9STRA)

HSP 1 Score: 103 bits (258), Expect = 1.800e-23
Identity = 54/117 (46.15%), Postives = 71/117 (60.68%), Query Frame = 1 
Query:    1 EGWQDALAEIVRSVTKTETKILSVCGKELSLAGFDDLAKEAYMKMDDFTNVMALYVKNQNWSEAVKLSEEHGRKYDDAVFLPYALWLRDKGRFDEALEAFQRAGRRDLSGAMTEQLM 351
            EGWQ  L E+              CG     AG  +LA E   K++D++ +MAL+V+ Q+W  AVKLSEEH  K+D  VFLPYA WL   GR+D+AL+A++RAGR DLS     QL+
Sbjct:  785 EGWQTVLIEL--------------CGDTFVAAGEHELALEVLSKLEDYSALMALHVQRQDWVAAVKLSEEHAGKFDQKVFLPYAEWLAATGRYDDALDAYRRAGRADLSLRTLRQLI 887
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BLAST of mRNA_Ecto-sp13_S_contig15723.4222.1 vs. uniprot
Match: F0XY87_AURAN (Intraflagellar transport protein 122 homolog n=1 Tax=Aureococcus anophagefferens TaxID=44056 RepID=F0XY87_AURAN)

HSP 1 Score: 102 bits (255), Expect = 4.570e-23
Identity = 53/123 (43.09%), Postives = 76/123 (61.79%), Query Frame = 1 
Query:    4 GWQDALAEIVRSVTKTETKILSVCGKELSLAGFDDLAKEAYMKMDDFTNVMALYVKNQNWSEAVKLSEE--------HGRKYDDAVFLPYALWLRDKGRFDEALEAFQRAGRRDLSGAMTEQL 348
             W + +A I RSVT  +  +L  C +    AG D LA+E Y K+DD   ++ LYV+ Q W+EA+KL+E+        H  K+D  ++LPYA WL    RFDEALEA++++GR D++  M EQL
Sbjct:  776 NWTEQMAAIARSVTGED--VLRQCARCFLAAGEDALAREVYAKLDDVEALLQLYVRKQQWAEAIKLAEDASAKFSNAHRAKFDQTLYLPYAEWLALHDRFDEALEAYRKSGRPDMAQHMMEQL 896
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BLAST of mRNA_Ecto-sp13_S_contig15723.4222.1 vs. uniprot
Match: A0A7S2W302_9STRA (Intraflagellar transport protein 122 homolog n=2 Tax=labyrinthulid quahog parasite QPX TaxID=96639 RepID=A0A7S2W302_9STRA)

HSP 1 Score: 96.3 bits (238), Expect = 8.900e-21
Identity = 49/115 (42.61%), Postives = 68/115 (59.13%), Query Frame = 1 
Query:    4 GWQDALAEIVRSVTKTETKILSVCGKELSLAGFDDLAKEAYMKMDDFTNVMALYVKNQNWSEAVKLSEEHGRKYDDAVFLPYALWLRDKGRFDEALEAFQRAGRRDLSGAMTEQL 348
            G  D L E+ R V K +T  L  C   L   G    AKE Y+KMDD  ++M L+V+   W +A  LSE+H  KY + V+LPYA WL ++ RFDEA  AF++A + + S  M ++L
Sbjct:  596 GCLDELIEVARIVDKKDTSALKKCAMYLREYGHHQYAKEVYLKMDDIQSLMKLHVELNKWEDAFLLSEQHKNKYAEDVYLPYARWLAEEDRFDEAQVAFKKAKQPEKSMMMLKEL 710
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BLAST of mRNA_Ecto-sp13_S_contig15723.4222.1 vs. uniprot
Match: UPI0010FC96A8 (intraflagellar transport protein 122 homolog isoform X1 n=4 Tax=Scleractinia TaxID=6125 RepID=UPI0010FC96A8)

HSP 1 Score: 93.2 bits (230), Expect = 1.080e-19
Identity = 50/118 (42.37%), Postives = 75/118 (63.56%), Query Frame = 1 
Query:    4 GWQDALAEIVRSVTKTETKILSVCG---KELSLAGFDDLAKEAYMKMDDFTNVMALYVKNQNWSEAVKLSEEHGRKYDDAVFLPYALWLRDKGRFDEALEAFQRAGRRDLSGAMTEQL 348
            GW + L E+ R++ K +T+ L  C    K+L   G+   A E Y+K+ D   ++ L+V++++W EA +L+E+H    DD VF+PYA WL +  RFDEA EAF +AGRRD +  + EQL
Sbjct:  737 GWVEKLLEVGRNLNKADTEGLKQCAFYFKQLDQHGY---ASEMYIKLGDMKALLELHVESKHWDEAFELAEKHPEFKDD-VFVPYANWLAENDRFDEAQEAFHKAGRRDEAVKVLEQL 850
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BLAST of mRNA_Ecto-sp13_S_contig15723.4222.1 vs. uniprot
Match: UPI0003C18954 (intraflagellar transport protein 122 homolog n=1 Tax=Latimeria chalumnae TaxID=7897 RepID=UPI0003C18954)

HSP 1 Score: 92.0 bits (227), Expect = 2.720e-19
Identity = 48/115 (41.74%), Postives = 65/115 (56.52%), Query Frame = 1 
Query:    4 GWQDALAEIVRSVTKTETKILSVCGKELSLAGFDDLAKEAYMKMDDFTNVMALYVKNQNWSEAVKLSEEHGRKYDDAVFLPYALWLRDKGRFDEALEAFQRAGRRDLSGAMTEQL 348
            GW D L +I R + K E + LS C            A E Y KM D   ++ LYV+ Q W EA  L E+H  ++ D VF+PYA WL +  RF+EA +AF +AGR+D +  + EQL
Sbjct:  634 GWADMLIDIARKLDKAEREPLSKCALYFKKLEHHGYAAETYTKMGDLRALVRLYVETQQWDEAFALVEKH-LEFKDEVFVPYAQWLAENDRFEEAQKAFHKAGRQDEAVKVLEQL 747
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BLAST of mRNA_Ecto-sp13_S_contig15723.4222.1 vs. uniprot
Match: H3AZ66_LATCH (Intraflagellar transport protein 122 homolog n=1 Tax=Latimeria chalumnae TaxID=7897 RepID=H3AZ66_LATCH)

HSP 1 Score: 92.0 bits (227), Expect = 2.720e-19
Identity = 48/115 (41.74%), Postives = 65/115 (56.52%), Query Frame = 1 
Query:    4 GWQDALAEIVRSVTKTETKILSVCGKELSLAGFDDLAKEAYMKMDDFTNVMALYVKNQNWSEAVKLSEEHGRKYDDAVFLPYALWLRDKGRFDEALEAFQRAGRRDLSGAMTEQL 348
            GW D L +I R + K E + LS C            A E Y KM D   ++ LYV+ Q W EA  L E+H  ++ D VF+PYA WL +  RF+EA +AF +AGR+D +  + EQL
Sbjct:  662 GWADMLIDIARKLDKAEREPLSKCALYFKKLEHHGYAAETYTKMGDLRALVRLYVETQQWDEAFALVEKH-LEFKDEVFVPYAQWLAENDRFEEAQKAFHKAGRQDEAVKVLEQL 775
The following BLAST results are available for this feature:
BLAST of mRNA_Ecto-sp13_S_contig15723.4222.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A6H5KTV2_9PHAE2.570e-7598.29Uncharacterized protein n=1 Tax=Ectocarpus sp. CCA... [more]
D7FXW9_ECTSI3.550e-6998.29Intraflagellar transport protein 122 homolog n=1 T... [more]
A0A812QAW2_SYMMI9.490e-2544.35Ift122 protein n=1 Tax=Symbiodinium microadriaticu... [more]
A0A7S1UEE7_9STRA3.740e-2443.10Hypothetical protein (Fragment) n=1 Tax=Phaeomonas... [more]
A0A835ZL39_9STRA1.800e-2346.15Intraflagellar transport protein 122 homolog n=1 T... [more]
F0XY87_AURAN4.570e-2343.09Intraflagellar transport protein 122 homolog n=1 T... [more]
A0A7S2W302_9STRA8.900e-2142.61Intraflagellar transport protein 122 homolog n=2 T... [more]
UPI0010FC96A81.080e-1942.37intraflagellar transport protein 122 homolog isofo... [more]
UPI0003C189542.720e-1941.74intraflagellar transport protein 122 homolog n=1 T... [more]
H3AZ66_LATCH2.720e-1941.74Intraflagellar transport protein 122 homolog n=1 T... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
Ecto-sp13_S_contig15723contigEcto-sp13_S_contig15723:1281..2140 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Ectocarpus species13 EcNAP12_S_4_19m OGS1.02022-07-08
Properties
Property NameValue
Taxonomic scopeEukaryota
Stop1
Start0
Seed ortholog score235.3
Seed ortholog evalue1.4e-59
Seed eggNOG ortholog2880.D7FXW9
Preferred nameIFT122
Model size354
KEGG koko:K16547,ko:K19656
Hectar predicted targeting categoryother localisation
GOsGO:0001654,GO:0001838,GO:0001841,GO:0001843,GO:0002009,GO:0003002,GO:0005575,GO:0005622,GO:0005623,GO:0005634,GO:0005654,GO:0005737,GO:0005815,GO:0005829,GO:0005856,GO:0005929,GO:0006810,GO:0006928,GO:0006996,GO:0007017,GO:0007018,GO:0007154,GO:0007165,GO:0007166,GO:0007224,GO:0007227,GO:0007275,GO:0007389,GO:0007399,GO:0007417,GO:0007423,GO:0007507,GO:0008104,GO:0008150,GO:0008285,GO:0008589,GO:0009653,GO:0009790,GO:0009792,GO:0009887,GO:0009888,GO:0009953,GO:0009966,GO:0009968,GO:0009987,GO:0010171,GO:0010172,GO:0010646,GO:0010648,GO:0010970,GO:0014020,GO:0015630,GO:0016043,GO:0016331,GO:0021510,GO:0021511,GO:0021513,GO:0021532,GO:0021904,GO:0021914,GO:0021915,GO:0022607,GO:0023051,GO:0023052,GO:0023057,GO:0030030,GO:0030031,GO:0030326,GO:0030705,GO:0030990,GO:0030991,GO:0031503,GO:0031974,GO:0031981,GO:0032391,GO:0032501,GO:0032502,GO:0032991,GO:0033036,GO:0033365,GO:0034613,GO:0035050,GO:0035107,GO:0035108,GO:0035113,GO:0035115,GO:0035136,GO:0035148,GO:0035239,GO:0035295,GO:0035556,GO:0035720,GO:0035721,GO:0035735,GO:0035869,GO:0036064,GO:0042073,GO:0042127,GO:0042733,GO:0042995,GO:0043005,GO:0043009,GO:0043010,GO:0043113,GO:0043226,GO:0043227,GO:0043228,GO:0043229,GO:0043231,GO:0043232,GO:0043233,GO:0044085,GO:0044422,GO:0044424,GO:0044428,GO:0044430,GO:0044441,GO:0044444,GO:0044446,GO:0044463,GO:0044464,GO:0044782,GO:0045184,GO:0045879,GO:0046626,GO:0046907,GO:0048513,GO:0048518,GO:0048519,GO:0048523,GO:0048568,GO:0048580,GO:0048582,GO:0048583,GO:0048585,GO:0048592,GO:0048593,GO:0048598,GO:0048646,GO:0048729,GO:0048731,GO:0048736,GO:0048856,GO:0050678,GO:0050680,GO:0050789,GO:0050793,GO:0050794,GO:0050896,GO:0051093,GO:0051094,GO:0051179,GO:0051234,GO:0051239,GO:0051240,GO:0051241,GO:0051641,GO:0051649,GO:0051668,GO:0051716,GO:0051960,GO:0051961,GO:0060173,GO:0060271,GO:0060429,GO:0060562,GO:0060606,GO:0060830,GO:0060831,GO:0060971,GO:0060972,GO:0061062,GO:0061063,GO:0061065,GO:0061066,GO:0061512,GO:0065007,GO:0070013,GO:0070727,GO:0070925,GO:0071840,GO:0072175,GO:0072359,GO:0072594,GO:0072657,GO:0090596,GO:0097458,GO:0097499,GO:0097500,GO:0097542,GO:0097546,GO:0097730,GO:0097731,GO:0097733,GO:0099111,GO:0120025,GO:0120031,GO:0120036,GO:0120038,GO:1900076,GO:1903441,GO:1905515,GO:1990778,GO:2000026
Exons3
EggNOG free text desc.ciliary receptor clustering involved in smoothened signaling pathway
EggNOG OGsCOG2319@1,KOG1538@2759
Cds size354
COG Functional cat.L
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko03036,ko04812
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1681462831.5693815-CDS-Ecto-sp13_S_contig15723:1280..13151681462831.5693815-CDS-Ecto-sp13_S_contig15723:1280..1315Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig15723 1281..1315 +
1681462831.5842679-CDS-Ecto-sp13_S_contig15723:1432..15741681462831.5842679-CDS-Ecto-sp13_S_contig15723:1432..1574Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig15723 1433..1574 +
1681462831.593005-CDS-Ecto-sp13_S_contig15723:1963..21401681462831.593005-CDS-Ecto-sp13_S_contig15723:1963..2140Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig15723 1964..2140 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_Ecto-sp13_S_contig15723.4222.1prot_Ecto-sp13_S_contig15723.4222.1Ectocarpus species13 EcNAP12_S_4_19mpolypeptideEcto-sp13_S_contig15723 1281..2140 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_Ecto-sp13_S_contig15723.4222.1

>prot_Ecto-sp13_S_contig15723.4222.1 ID=prot_Ecto-sp13_S_contig15723.4222.1|Name=mRNA_Ecto-sp13_S_contig15723.4222.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=polypeptide|length=118bp
EGWQDALAEIVRSVTKTETKILSVCGKELSLAGFDDLAKEAYMKMDDFTN
VMALYVKNQNWSEAVKLSEEHGRKYDDAVFLPYALWLRDKGRFDEALEAF
QRAGRRDLSGAMTEQLM*
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mRNA from alignment at Ecto-sp13_S_contig15723:1281..2140+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_Ecto-sp13_S_contig15723.4222.1 ID=mRNA_Ecto-sp13_S_contig15723.4222.1|Name=mRNA_Ecto-sp13_S_contig15723.4222.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=mRNA|length=860bp|location=Sequence derived from alignment at Ecto-sp13_S_contig15723:1281..2140+ (Ectocarpus species13 EcNAP12_S_4_19m)
GAAGGTTGGCAGGATGCTCTGGCAGAGATAGTCAGGTGAGAAAACGACAG
TGAAGACAGGTACAACTGCTACTCTCCCCTTCGTCAGCACTCCCCTGAGC
ACGCAGGACACGAAACTCATGATCCTGATGAATTGACACGTCGGTGTGGC
AGGTCGGTCACGAAGACGGAGACGAAAATCCTCAGTGTTTGCGGCAAGGA
GTTGTCCTTGGCGGGTTTCGACGACCTTGCGAAGGAGGCGTACATGAAGA
TGGACGACTTCACGAACGTCATGGCACTTTACGTCAAAAACCAGGTCGGT
TTTTCTTCTCTCTTGTGCTATGTATGTCACACGTTCTGTTGGGGTCAAAG
TGGATATTAACTAAAACGCTCGCGAAACAAGCATGGAATTCCGGTGGAAT
AATGAGAGTCACCCAATGGAAATAACCATGGTAGTCCCACGAAGCCATTG
TACGTTCCAAGGGAAGGCACAAGGTGACTGTTCTTTCAGTCTTATTCAAC
AGGTTGGAAGCGCGCAGTACACGTGCAGCATGTGATGGAGTATATATCGA
AGGGGGCTTTCACAGACGAGGTGAGAAGGAGATCAGCGAACAAGTACAAA
GGCTACACCTGTTTGGCTGTGTCGGTATCTCATGTTCTTCTATCCATTTC
TCGCTCATGTGGCGTTGGTACTGCGGCGATTAGAACTGGTCGGAAGCGGT
GAAGCTTTCCGAGGAGCACGGCCGCAAGTACGACGACGCCGTTTTCCTAC
CCTACGCGCTGTGGCTCCGAGACAAGGGGAGGTTTGACGAAGCCCTCGAG
GCGTTCCAGAGGGCCGGAAGGCGGGATTTGAGCGGGGCCATGACTGAGCA
ACTCATGTGA
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Coding sequence (CDS) from alignment at Ecto-sp13_S_contig15723:1281..2140+

>mRNA_Ecto-sp13_S_contig15723.4222.1 ID=mRNA_Ecto-sp13_S_contig15723.4222.1|Name=mRNA_Ecto-sp13_S_contig15723.4222.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=CDS|length=354bp|location=Sequence derived from alignment at Ecto-sp13_S_contig15723:1281..2140+ (Ectocarpus species13 EcNAP12_S_4_19m)
GAAGGTTGGCAGGATGCTCTGGCAGAGATAGTCAGGTCGGTCACGAAGAC
GGAGACGAAAATCCTCAGTGTTTGCGGCAAGGAGTTGTCCTTGGCGGGTT
TCGACGACCTTGCGAAGGAGGCGTACATGAAGATGGACGACTTCACGAAC
GTCATGGCACTTTACGTCAAAAACCAGAACTGGTCGGAAGCGGTGAAGCT
TTCCGAGGAGCACGGCCGCAAGTACGACGACGCCGTTTTCCTACCCTACG
CGCTGTGGCTCCGAGACAAGGGGAGGTTTGACGAAGCCCTCGAGGCGTTC
CAGAGGGCCGGAAGGCGGGATTTGAGCGGGGCCATGACTGAGCAACTCAT
GTGA
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