mRNA_Ecto-sp13_S_contig15574.4143.1 (mRNA) Ectocarpus species13 EcNAP12_S_4_19m

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_Ecto-sp13_S_contig15574.4143.1
Unique NamemRNA_Ecto-sp13_S_contig15574.4143.1
TypemRNA
OrganismEctocarpus species13 EcNAP12_S_4_19m (Ectocarpus species13 EcNAP12_S_4_19m)
Homology
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BLAST of mRNA_Ecto-sp13_S_contig15574.4143.1 vs. uniprot
Match: D8LP07_ECTSI (Possible CDPK n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LP07_ECTSI)

HSP 1 Score: 174 bits (440), Expect = 2.870e-54
Identity = 80/80 (100.00%), Postives = 80/80 (100.00%), Query Frame = 1 
Query:    1 MGGFVSTQCGTPGYVAPEILRAESYGTSVDMWSIGVIVYILLGGYPPFHDENQTRLFRKIKAGNFKFHPEYWQSISNEAK 240
            MGGFVSTQCGTPGYVAPEILRAESYGTSVDMWSIGVIVYILLGGYPPFHDENQTRLFRKIKAGNFKFHPEYWQSISNEAK
Sbjct:    1 MGGFVSTQCGTPGYVAPEILRAESYGTSVDMWSIGVIVYILLGGYPPFHDENQTRLFRKIKAGNFKFHPEYWQSISNEAK 80
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BLAST of mRNA_Ecto-sp13_S_contig15574.4143.1 vs. uniprot
Match: A0A6H5K8A8_9PHAE (Protein kinase domain-containing protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5K8A8_9PHAE)

HSP 1 Score: 170 bits (430), Expect = 3.510e-50
Identity = 78/80 (97.50%), Postives = 79/80 (98.75%), Query Frame = 1 
Query:    1 MGGFVSTQCGTPGYVAPEILRAESYGTSVDMWSIGVIVYILLGGYPPFHDENQTRLFRKIKAGNFKFHPEYWQSISNEAK 240
            MGGFVSTQCGTPGYVAPEILRAESYGTSVDMWSIGVIVYILLGGYPPFHDENQTRLFRKIKAGNFKFHPEYWQS S+EAK
Sbjct:  211 MGGFVSTQCGTPGYVAPEILRAESYGTSVDMWSIGVIVYILLGGYPPFHDENQTRLFRKIKAGNFKFHPEYWQSTSSEAK 290
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BLAST of mRNA_Ecto-sp13_S_contig15574.4143.1 vs. uniprot
Match: A0A835YRH8_9STRA (Kinase-like domain-containing protein n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835YRH8_9STRA)

HSP 1 Score: 143 bits (361), Expect = 2.690e-42
Identity = 63/77 (81.82%), Postives = 70/77 (90.91%), Query Frame = 1 
Query:   10 FVSTQCGTPGYVAPEILRAESYGTSVDMWSIGVIVYILLGGYPPFHDENQTRLFRKIKAGNFKFHPEYWQSISNEAK 240
             V+TQCGTP YVAPEILR + YGTSVDMWSIGVI+YILLGGYPPFHDENQTRLFRKI+AG++KFH EYW  +SNEAK
Sbjct:    1 MVTTQCGTPAYVAPEILRHKPYGTSVDMWSIGVIIYILLGGYPPFHDENQTRLFRKIRAGSYKFHSEYWGGVSNEAK 77
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BLAST of mRNA_Ecto-sp13_S_contig15574.4143.1 vs. uniprot
Match: A0A6H5KCN7_9PHAE (Protein kinase domain-containing protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5KCN7_9PHAE)

HSP 1 Score: 140 bits (353), Expect = 4.680e-40
Identity = 63/80 (78.75%), Postives = 70/80 (87.50%), Query Frame = 1 
Query:    1 MGGFVSTQCGTPGYVAPEILRAESYGTSVDMWSIGVIVYILLGGYPPFHDENQTRLFRKIKAGNFKFHPEYWQSISNEAK 240
            + G VS QCG+PGYVAPEILRA  YGTSVDMWS+GVI+Y LLGGYPPFHDENQTRLFR+IKAG+FKFH EYW + S EAK
Sbjct:   78 LNGPVSEQCGSPGYVAPEILRALPYGTSVDMWSVGVIIYTLLGGYPPFHDENQTRLFRRIKAGSFKFHDEYWSNTSLEAK 157
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BLAST of mRNA_Ecto-sp13_S_contig15574.4143.1 vs. uniprot
Match: A0A835YZ60_9STRA (Kinase-like domain-containing protein n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835YZ60_9STRA)

HSP 1 Score: 143 bits (360), Expect = 6.440e-40
Identity = 64/79 (81.01%), Postives = 70/79 (88.61%), Query Frame = 1 
Query:    4 GGFVSTQCGTPGYVAPEILRAESYGTSVDMWSIGVIVYILLGGYPPFHDENQTRLFRKIKAGNFKFHPEYWQSISNEAK 240
            G  V+TQCGTP YVAPEILR   YGTSVDMWSIGVI+YILLGGYPPFHDENQTRLFRKI+AG F+FH EYW SIS++AK
Sbjct:  194 GSMVTTQCGTPAYVAPEILRHRPYGTSVDMWSIGVIIYILLGGYPPFHDENQTRLFRKIRAGKFEFHNEYWGSISSDAK 272
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BLAST of mRNA_Ecto-sp13_S_contig15574.4143.1 vs. uniprot
Match: D8LK21_ECTSI (Possible Ca2+/calmodulin-dependent protein kinase, EF-Hand protein superfamily n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LK21_ECTSI)

HSP 1 Score: 140 bits (353), Expect = 1.690e-38
Identity = 63/80 (78.75%), Postives = 70/80 (87.50%), Query Frame = 1 
Query:    1 MGGFVSTQCGTPGYVAPEILRAESYGTSVDMWSIGVIVYILLGGYPPFHDENQTRLFRKIKAGNFKFHPEYWQSISNEAK 240
            + G VS QCG+PGYVAPEILRA  YGTSVDMWS+GVI+Y LLGGYPPFHDENQTRLFR+IKAG+FKFH EYW + S EAK
Sbjct:  235 LNGPVSEQCGSPGYVAPEILRALPYGTSVDMWSVGVIIYTLLGGYPPFHDENQTRLFRRIKAGSFKFHDEYWSNTSLEAK 314
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BLAST of mRNA_Ecto-sp13_S_contig15574.4143.1 vs. uniprot
Match: D8LEF0_ECTSI (Myosin light chain kinase (MLCK) n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LEF0_ECTSI)

HSP 1 Score: 138 bits (348), Expect = 2.010e-36
Identity = 58/80 (72.50%), Postives = 71/80 (88.75%), Query Frame = 1 
Query:    1 MGGFVSTQCGTPGYVAPEILRAESYGTSVDMWSIGVIVYILLGGYPPFHDENQTRLFRKIKAGNFKFHPEYWQSISNEAK 240
            + G+V+ QCGTPGYVAPEILR+  YGTSVDMWSIGVI+YI+L GYPPFHDE+Q RL+RKIKAG+++F PEYW  +S+EAK
Sbjct:  531 LDGYVTDQCGTPGYVAPEILRSRPYGTSVDMWSIGVIIYIILAGYPPFHDEDQNRLYRKIKAGHYRFDPEYWNDVSSEAK 610
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BLAST of mRNA_Ecto-sp13_S_contig15574.4143.1 vs. uniprot
Match: A0A7S0SZL7_9STRA (Hypothetical protein n=1 Tax=Chromulina nebulosa TaxID=96789 RepID=A0A7S0SZL7_9STRA)

HSP 1 Score: 129 bits (325), Expect = 1.870e-35
Identity = 53/77 (68.83%), Postives = 66/77 (85.71%), Query Frame = 1 
Query:   10 FVSTQCGTPGYVAPEILRAESYGTSVDMWSIGVIVYILLGGYPPFHDENQTRLFRKIKAGNFKFHPEYWQSISNEAK 240
             +STQCGTPGY+APEIL  + YG +VDMWSIGVI YI+LGGYPPFHD+N   LFRKI+ G+F+FHPEYW ++S++AK
Sbjct:  102 LLSTQCGTPGYIAPEILENKPYGKAVDMWSIGVITYIILGGYPPFHDDNMRNLFRKIRKGDFEFHPEYWNNVSSDAK 178
[Close]
BLAST of mRNA_Ecto-sp13_S_contig15574.4143.1 vs. uniprot
Match: A0A6H5K8I6_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5K8I6_9PHAE)

HSP 1 Score: 133 bits (335), Expect = 1.270e-34
Identity = 57/80 (71.25%), Postives = 69/80 (86.25%), Query Frame = 1 
Query:    1 MGGFVSTQCGTPGYVAPEILRAESYGTSVDMWSIGVIVYILLGGYPPFHDENQTRLFRKIKAGNFKFHPEYWQSISNEAK 240
            + G+V+ QCGTPGYVAPEILR   YGTSVDMWSIGVI+YI+L GYPPF DE+Q RL+RKIKAG+++F PEYW  +S+EAK
Sbjct:  526 LDGYVTDQCGTPGYVAPEILRGHPYGTSVDMWSIGVIIYIILAGYPPFCDEDQHRLYRKIKAGHYRFEPEYWNDVSSEAK 605
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BLAST of mRNA_Ecto-sp13_S_contig15574.4143.1 vs. uniprot
Match: A0A6V1U2B4_HETAK (Hypothetical protein n=1 Tax=Heterosigma akashiwo TaxID=2829 RepID=A0A6V1U2B4_HETAK)

HSP 1 Score: 129 bits (323), Expect = 1.580e-34
Identity = 54/79 (68.35%), Postives = 66/79 (83.54%), Query Frame = 1 
Query:    4 GGFVSTQCGTPGYVAPEILRAESYGTSVDMWSIGVIVYILLGGYPPFHDENQTRLFRKIKAGNFKFHPEYWQSISNEAK 240
            G  ++TQCGTPGYVAPEIL    YG SVDMWS GVI YILLGGYPPFHDE Q+ LF KI++G+++FHPEYWQ++S +A+
Sbjct:  182 GNTLTTQCGTPGYVAPEILNGVPYGKSVDMWSCGVITYILLGGYPPFHDEKQSNLFAKIRSGSYEFHPEYWQNVSKDAQ 260
The following BLAST results are available for this feature:
BLAST of mRNA_Ecto-sp13_S_contig15574.4143.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
D8LP07_ECTSI2.870e-54100.00Possible CDPK n=1 Tax=Ectocarpus siliculosus TaxID... [more]
A0A6H5K8A8_9PHAE3.510e-5097.50Protein kinase domain-containing protein n=1 Tax=E... [more]
A0A835YRH8_9STRA2.690e-4281.82Kinase-like domain-containing protein n=1 Tax=Trib... [more]
A0A6H5KCN7_9PHAE4.680e-4078.75Protein kinase domain-containing protein n=1 Tax=E... [more]
A0A835YZ60_9STRA6.440e-4081.01Kinase-like domain-containing protein n=1 Tax=Trib... [more]
D8LK21_ECTSI1.690e-3878.75Possible Ca2+/calmodulin-dependent protein kinase,... [more]
D8LEF0_ECTSI2.010e-3672.50Myosin light chain kinase (MLCK) n=1 Tax=Ectocarpu... [more]
A0A7S0SZL7_9STRA1.870e-3568.83Hypothetical protein n=1 Tax=Chromulina nebulosa T... [more]
A0A6H5K8I6_9PHAE1.270e-3471.25Uncharacterized protein n=1 Tax=Ectocarpus sp. CCA... [more]
A0A6V1U2B4_HETAK1.580e-3468.35Hypothetical protein n=1 Tax=Heterosigma akashiwo ... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
Ecto-sp13_S_contig15574contigEcto-sp13_S_contig15574:3470..4112 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Ectocarpus species13 EcNAP12_S_4_19m OGS1.02022-07-08
Properties
Property NameValue
Taxonomic scopeEukaryota
Stop0
Start1
Seed ortholog score127.5
Seed ortholog evalue2.8e-27
Seed eggNOG ortholog2880.D8LE50
Model size240
KEGG koko:K04373,ko:K04445
KEGG Pathwayko04010,ko04114,ko04150,ko04261,ko04668,ko04713,ko04714,ko04720,ko04722,ko04914,ko04931,ko05200,ko05206,ko05219,map04010,map04114,map04150,map04261,map04668,map04713,map04714,map04720,map04722,map04914,map04931,map05200,map05206,map05219
Hectar predicted targeting categoryother localisation
Exons2
EggNOG free text desc.protein serine/threonine kinase activity
EggNOG OGsKOG0603@1,KOG0603@2759
Ec32 ortholog descriptionpossible CDPK
Ec32 orthologEc-06_007050.1
EC2.7.11.1
Cds size240
COG Functional cat.G
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko01000,ko01001,ko03019
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1681462825.4827104-CDS-Ecto-sp13_S_contig15574:3469..36281681462825.4827104-CDS-Ecto-sp13_S_contig15574:3469..3628Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig15574 3470..3628 -
1681462825.495095-CDS-Ecto-sp13_S_contig15574:4031..41121681462825.495095-CDS-Ecto-sp13_S_contig15574:4031..4112Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig15574 4032..4112 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_Ecto-sp13_S_contig15574.4143.1prot_Ecto-sp13_S_contig15574.4143.1Ectocarpus species13 EcNAP12_S_4_19mpolypeptideEcto-sp13_S_contig15574 3470..4112 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_Ecto-sp13_S_contig15574.4143.1

>prot_Ecto-sp13_S_contig15574.4143.1 ID=prot_Ecto-sp13_S_contig15574.4143.1|Name=mRNA_Ecto-sp13_S_contig15574.4143.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=polypeptide|length=80bp
MGGFVSTQCGTPGYVAPEILRAESYGTSVDMWSIGVIVYILLGGYPPFHD
ENQTRLFRKIKAGNFKFHPEYWQSISNEAK
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mRNA from alignment at Ecto-sp13_S_contig15574:3470..4112-

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_Ecto-sp13_S_contig15574.4143.1 ID=mRNA_Ecto-sp13_S_contig15574.4143.1|Name=mRNA_Ecto-sp13_S_contig15574.4143.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=mRNA|length=643bp|location=Sequence derived from alignment at Ecto-sp13_S_contig15574:3470..4112- (Ectocarpus species13 EcNAP12_S_4_19m)
ATGGGCGGCTTCGTGTCGACGCAGTGCGGGACCCCTGGCTACGTGGCTCC
CGAGATCCTCCGGGCAGAGTCTTACGGCACTGTGAGTTTTTTTGTTTGTT
TGTTCTTGTGCCAGTTTTTCTCAAAAGTGTTGGAATACCATCTGCTCATG
GTAGTGTTTGGGGTACTTGATCTAGAGATGATGTGCGGGGCCACTGGACG
TTTGGGTGAGGTAACCTTTGACCACCCACACGTGTTTCGCCCGCAAGAAA
CCGATCAACCGACACACCTGTATGTAGTGCTTTTCACCTACCGCTCTTTG
CGGCGGAATGATCGCATTACAGCCGTCTTTTTTTTACACCACAGTCCGGT
TCTTAGCCTCGCCTCTCTTGATGATCGTTTATCGTTTATCTACCTTCGCT
TTTTTTGCTCGGGCCCCACAATCCGTCCAACCATCACCCGGCGGTTCTCA
CGGACAACGAACGAACGAACGAACGTACCACCAGTCCGTGGACATGTGGA
GCATCGGCGTGATCGTCTACATCCTCCTGGGCGGCTACCCTCCCTTCCAC
GACGAGAACCAGACGAGGCTCTTCCGGAAAATCAAGGCCGGCAACTTCAA
GTTCCACCCGGAGTACTGGCAATCCATCTCTAACGAAGCCAAG
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Coding sequence (CDS) from alignment at Ecto-sp13_S_contig15574:3470..4112-

>mRNA_Ecto-sp13_S_contig15574.4143.1 ID=mRNA_Ecto-sp13_S_contig15574.4143.1|Name=mRNA_Ecto-sp13_S_contig15574.4143.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=CDS|length=240bp|location=Sequence derived from alignment at Ecto-sp13_S_contig15574:3470..4112- (Ectocarpus species13 EcNAP12_S_4_19m)
ATGGGCGGCTTCGTGTCGACGCAGTGCGGGACCCCTGGCTACGTGGCTCC
CGAGATCCTCCGGGCAGAGTCTTACGGCACTTCCGTGGACATGTGGAGCA
TCGGCGTGATCGTCTACATCCTCCTGGGCGGCTACCCTCCCTTCCACGAC
GAGAACCAGACGAGGCTCTTCCGGAAAATCAAGGCCGGCAACTTCAAGTT
CCACCCGGAGTACTGGCAATCCATCTCTAACGAAGCCAAG
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