mRNA_Ecto-sp13_S_contig1548.4091.1 (mRNA) Ectocarpus species13 EcNAP12_S_4_19m

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_Ecto-sp13_S_contig1548.4091.1
Unique NamemRNA_Ecto-sp13_S_contig1548.4091.1
TypemRNA
OrganismEctocarpus species13 EcNAP12_S_4_19m (Ectocarpus species13 EcNAP12_S_4_19m)
Homology
BLAST of mRNA_Ecto-sp13_S_contig1548.4091.1 vs. uniprot
Match: D8LRB7_ECTSI (Similar to calmodulin 2 n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LRB7_ECTSI)

HSP 1 Score: 81.3 bits (199), Expect = 1.500e-16
Identity = 43/70 (61.43%), Postives = 44/70 (62.86%), Query Frame = 1
Query:  160 QNTRAEFEDAFRAIDHNKDGKVDITELNRLMVATGFGLTNQELKEFFGFFDRDGSGKVDMVEFLDYMMDA 369
            +NTRAEF                       MVATGFGLTNQELKE FGFFD DGSGKVDM EFLDYMMDA
Sbjct:  134 KNTRAEFXXXXXXXXXXXXXXXXXXXXXXXMVATGFGLTNQELKEIFGFFDSDGSGKVDMAEFLDYMMDA 203          
BLAST of mRNA_Ecto-sp13_S_contig1548.4091.1 vs. uniprot
Match: A0A6H5JR81_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JR81_9PHAE)

HSP 1 Score: 76.3 bits (186), Expect = 1.180e-14
Identity = 35/39 (89.74%), Postives = 37/39 (94.87%), Query Frame = 1
Query:  253 VATGFGLTNQELKEFFGFFDRDGSGKVDMVEFLDYMMDA 369
            VA+GFGLTN+ELKE FGFFDRDGSGKVDM EFLDYMMDA
Sbjct:  162 VASGFGLTNRELKEIFGFFDRDGSGKVDMAEFLDYMMDA 200          
BLAST of mRNA_Ecto-sp13_S_contig1548.4091.1 vs. uniprot
Match: A0A267FZ02_9PLAT (Uncharacterized protein (Fragment) n=4 Tax=Macrostomum lignano TaxID=282301 RepID=A0A267FZ02_9PLAT)

HSP 1 Score: 59.7 bits (143), Expect = 6.770e-8
Identity = 38/126 (30.16%), Postives = 63/126 (50.00%), Query Frame = 1
Query:    1 GAPGTKRSCRPVSSTTTILLRAGRRHNPRALRR---SHYFRLSPAASPWVPNTCEQQ--NTRAEFEDAFRAIDHNKDGKVDITELNRLMVATGFGLTNQELKEFFGFFDRDGSGKVDMVEFLDYMM 363
            G  G+K   R       ++ + GR  + RALR+    H  R +          C Q+   TR E  DAF A+D N DG + + E  +++ + G  +T  E +E    +D++G GK+D  EF+D+++
Sbjct:   48 GRDGSKGRLRTFDEFAALVQQTGRNPSQRALRQYWDRHNSRRTGVDLQSFLEVCRQEPVTTRDELLDAFHAVDLNSDGYLSLDEFVQMLSSGGEPITKLEARELLQQYDKNGDGKLDYEEFIDFVL 173          
BLAST of mRNA_Ecto-sp13_S_contig1548.4091.1 vs. uniprot
Match: UPI001E1E08FC (calmodulin-2/4-like isoform X3 n=1 Tax=Mercenaria mercenaria TaxID=6596 RepID=UPI001E1E08FC)

HSP 1 Score: 53.9 bits (128), Expect = 2.530e-6
Identity = 25/67 (37.31%), Postives = 42/67 (62.69%), Query Frame = 1
Query:  169 RAEFEDAFRAIDHNKDGKVDITELNRLMVATGFGLTNQELKEFFGFFDRDGSGKVDMVEFLDYMMDA 369
            R E +DAFR  D    G+++ TEL R++   G  LT+++ KE     D++G GKV+M +F+D++ + 
Sbjct:   89 REELKDAFRIFDRTGKGEINKTELERVLKKYGEPLTHRQSKELIELMDKNGDGKVNMDDFVDFLCEV 155          
BLAST of mRNA_Ecto-sp13_S_contig1548.4091.1 vs. uniprot
Match: UPI000DC838C1 (Uncharacterized protein n=1 Tax=Xenopus laevis TaxID=8355 RepID=UPI000DC838C1)

HSP 1 Score: 52.4 bits (124), Expect = 3.210e-6
Identity = 26/72 (36.11%), Postives = 42/72 (58.33%), Query Frame = 1
Query:  151 CEQQNT-RAEFEDAFRAIDHNKDGKVDITELNRLMVATGFGLTNQELKEFFGFFDRDGSGKVDMVEFLDYMM 363
            C+ QN    E +  F  IDH+KDGK+ I++L+      G  L+ QEL+E     D++G   +D+ EF++ M+
Sbjct:   39 CDGQNPPHKELKKGFSMIDHDKDGKITISDLSAASKMAGIHLSRQELEEMMAEADQNGDHAIDISEFIEIML 110          
BLAST of mRNA_Ecto-sp13_S_contig1548.4091.1 vs. uniprot
Match: A0A7S2CDS1_9DINO (Calmodulin (Fragment) n=1 Tax=Alexandrium andersonii TaxID=327968 RepID=A0A7S2CDS1_9DINO)

HSP 1 Score: 53.9 bits (128), Expect = 4.090e-6
Identity = 28/84 (33.33%), Postives = 47/84 (55.95%), Query Frame = 1
Query:  115 LSPAASPWVPNTCEQQNTRAEFEDAFRAIDHNKDGKVDITELNRLMVATGFGLTNQELKEFFGFFDRDGSGKVDMVEFLDYMMD 366
            L PA  P  P       +  + +  F+ +D NKDG +   E+ RL+      ++++ELK  FG  DR+G+G+V+  EF+D++ D
Sbjct:   14 LRPALEPMPPADA----SIGKIKKKFKKLDGNKDGSLSFAEMKRLLQKGRDDISDRELKILFGQVDRNGNGRVEFSEFVDFVFD 93          
BLAST of mRNA_Ecto-sp13_S_contig1548.4091.1 vs. uniprot
Match: A7T798_NEMVE (Predicted protein n=1 Tax=Nematostella vectensis TaxID=45351 RepID=A7T798_NEMVE)

HSP 1 Score: 52.4 bits (124), Expect = 6.820e-6
Identity = 25/62 (40.32%), Postives = 39/62 (62.90%), Query Frame = 1
Query:  175 EFEDAFRAIDHNKDGKVDITELNRLMVATGFGLTNQELKEFFGFFDRDGSGKVDMVEFLDYM 360
            EF++AF + D N DG++D  EL  +M + G    ++ELK      D+DGSG +D+ EF++ M
Sbjct:   16 EFKNAFMSFDKNGDGRIDAEELGIVMRSIGLHPKDEELKAMIKQADKDGSGDIDLPEFIELM 77          
BLAST of mRNA_Ecto-sp13_S_contig1548.4091.1 vs. uniprot
Match: A0A6P4NLX4_GOSAR (probable calcium-binding protein CML45 n=9 Tax=Gossypium TaxID=3633 RepID=A0A6P4NLX4_GOSAR)

HSP 1 Score: 51.6 bits (122), Expect = 7.410e-6
Identity = 27/65 (41.54%), Postives = 37/65 (56.92%), Query Frame = 1
Query:  175 EFEDAFRAIDHNKDGKVDITELNRLMVATGFG---LTNQELKEFFGFFDRDGSGKVDMVEFLDYM 360
            E ++AF   D NKDG +D TEL R+++  G     +T +E K      D DG G+VD  EFL +M
Sbjct:   54 EIKEAFDVFDDNKDGFIDATELQRVLICLGVKGKMVTVEECKRMIRVADEDGDGRVDFNEFLKFM 118          
BLAST of mRNA_Ecto-sp13_S_contig1548.4091.1 vs. uniprot
Match: UPI00138FC30A (calmodulin n=1 Tax=Nematostella vectensis TaxID=45351 RepID=UPI00138FC30A)

HSP 1 Score: 52.4 bits (124), Expect = 7.580e-6
Identity = 25/62 (40.32%), Postives = 39/62 (62.90%), Query Frame = 1
Query:  175 EFEDAFRAIDHNKDGKVDITELNRLMVATGFGLTNQELKEFFGFFDRDGSGKVDMVEFLDYM 360
            EF++AF + D N DG++D  EL  +M + G    ++ELK      D+DGSG +D+ EF++ M
Sbjct:   28 EFKNAFMSFDKNGDGRIDAEELGIVMRSIGLHPKDEELKAMIKQADKDGSGDIDLPEFIELM 89          
BLAST of mRNA_Ecto-sp13_S_contig1548.4091.1 vs. uniprot
Match: A0A1U8M7T1_GOSHI (probable calcium-binding protein CML45 n=14 Tax=Gossypium TaxID=3633 RepID=A0A1U8M7T1_GOSHI)

HSP 1 Score: 51.6 bits (122), Expect = 1.290e-5
Identity = 27/65 (41.54%), Postives = 37/65 (56.92%), Query Frame = 1
Query:  175 EFEDAFRAIDHNKDGKVDITELNRLMVATGFG---LTNQELKEFFGFFDRDGSGKVDMVEFLDYM 360
            E ++AF   D NKDG +D TEL R+++  G     +T +E K      D DG G+VD  EFL +M
Sbjct:   86 EIKEAFDVFDDNKDGFIDATELQRVLICLGVRGKMVTVEECKRMIRVADEDGDGRVDFNEFLKFM 150          
The following BLAST results are available for this feature:
BLAST of mRNA_Ecto-sp13_S_contig1548.4091.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 19
Match NameE-valueIdentityDescription
D8LRB7_ECTSI1.500e-1661.43Similar to calmodulin 2 n=1 Tax=Ectocarpus silicul... [more]
A0A6H5JR81_9PHAE1.180e-1489.74Uncharacterized protein n=1 Tax=Ectocarpus sp. CCA... [more]
A0A267FZ02_9PLAT6.770e-830.16Uncharacterized protein (Fragment) n=4 Tax=Macrost... [more]
UPI001E1E08FC2.530e-637.31calmodulin-2/4-like isoform X3 n=1 Tax=Mercenaria ... [more]
UPI000DC838C13.210e-636.11Uncharacterized protein n=1 Tax=Xenopus laevis Tax... [more]
A0A7S2CDS1_9DINO4.090e-633.33Calmodulin (Fragment) n=1 Tax=Alexandrium anderson... [more]
A7T798_NEMVE6.820e-640.32Predicted protein n=1 Tax=Nematostella vectensis T... [more]
A0A6P4NLX4_GOSAR7.410e-641.54probable calcium-binding protein CML45 n=9 Tax=Gos... [more]
UPI00138FC30A7.580e-640.32calmodulin n=1 Tax=Nematostella vectensis TaxID=45... [more]
A0A1U8M7T1_GOSHI1.290e-541.54probable calcium-binding protein CML45 n=14 Tax=Go... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
Ecto-sp13_S_contig1548contigEcto-sp13_S_contig1548:16015..16386 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Ectocarpus species13 EcNAP12_S_4_19m OGS1.02022-07-08
Properties
Property NameValue
Taxonomic scopeEukaryota
Stop1
Start0
Seed ortholog score83.2
Seed ortholog evalue9.5e-14
Seed eggNOG ortholog2880.D8LRB7
Preferred nameMLC2
Model size372
KEGG koko:K02183
KEGG Pathwayko04014,ko04015,ko04016,ko04020,ko04022,ko04024,ko04070,ko04114,ko04218,ko04261,ko04270,ko04371,ko04626,ko04713,ko04720,ko04722,ko04728,ko04740,ko04744,ko04745,ko04750,ko04910,ko04912,ko04915,ko04916,ko04921,ko04922,ko04924,ko04925,ko04970,ko04971,ko05010,ko05031,ko05034,ko05133,ko05152,ko05167,ko05200,ko05214,ko05418,map04014,map04015,map04016,map04020,map04022,map04024,map04070,map04114,map04218,map04261,map04270,map04371,map04626,map04713,map04720,map04722,map04728,map04740,map04744,map04745,map04750,map04910,map04912,map04915,map04916,map04921,map04922,map04924,map04925,map04970,map04971,map05010,map05031,map05034,map05133,map05152,map05167,map05200,map05214,map05418
Hectar predicted targeting categoryother localisation
GOsGO:0000142,GO:0000910,GO:0000916,GO:0003674,GO:0005488,GO:0005515,GO:0005575,GO:0005622,GO:0005623,GO:0005737,GO:0005826,GO:0005856,GO:0005933,GO:0005935,GO:0005938,GO:0006996,GO:0007010,GO:0007049,GO:0008092,GO:0008150,GO:0009987,GO:0015629,GO:0016043,GO:0016459,GO:0016460,GO:0017022,GO:0022402,GO:0030029,GO:0030036,GO:0030427,GO:0030863,GO:0030864,GO:0030865,GO:0030866,GO:0031032,GO:0032153,GO:0032155,GO:0032506,GO:0032991,GO:0036213,GO:0043226,GO:0043228,GO:0043229,GO:0043232,GO:0044422,GO:0044424,GO:0044430,GO:0044444,GO:0044446,GO:0044448,GO:0044464,GO:0044837,GO:0045159,GO:0051301,GO:0061640,GO:0070938,GO:0071840,GO:0071944,GO:0099568,GO:0110085
Exons1
EggNOG free text desc.Ca2 -binding protein (EF-Hand superfamily
EggNOG OGsCOG5126@1,KOG0027@2759
Ec32 ortholog descriptionEF-hand domain pair
Ec32 orthologEc-06_003120.1
Cds size372
COG Functional cat.DTZ
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko01009,ko03036,ko04131,ko04147
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1681462822.1305184-CDS-Ecto-sp13_S_contig1548:16014..163861681462822.1305184-CDS-Ecto-sp13_S_contig1548:16014..16386Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig1548 16015..16386 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_Ecto-sp13_S_contig1548.4091.1prot_Ecto-sp13_S_contig1548.4091.1Ectocarpus species13 EcNAP12_S_4_19mpolypeptideEcto-sp13_S_contig1548 16015..16386 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_Ecto-sp13_S_contig1548.4091.1

>prot_Ecto-sp13_S_contig1548.4091.1 ID=prot_Ecto-sp13_S_contig1548.4091.1|Name=mRNA_Ecto-sp13_S_contig1548.4091.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=polypeptide|length=124bp
GAPGTKRSCRPVSSTTTILLRAGRRHNPRALRRSHYFRLSPAASPWVPNT
CEQQNTRAEFEDAFRAIDHNKDGKVDITELNRLMVATGFGLTNQELKEFF
GFFDRDGSGKVDMVEFLDYMMDA*
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mRNA from alignment at Ecto-sp13_S_contig1548:16015..16386-

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_Ecto-sp13_S_contig1548.4091.1 ID=mRNA_Ecto-sp13_S_contig1548.4091.1|Name=mRNA_Ecto-sp13_S_contig1548.4091.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=mRNA|length=372bp|location=Sequence derived from alignment at Ecto-sp13_S_contig1548:16015..16386- (Ectocarpus species13 EcNAP12_S_4_19m)
GGGGCACCAGGCACGAAACGTTCGTGCAGGCCCGTATCCTCAACAACCAC TATTTTGCTACGGGCTGGTCGGCGGCACAATCCACGTGCACTTCGTCGTT CCCACTACTTTCGTCTCTCTCCCGCCGCTTCCCCATGGGTACCCAATACT TGCGAACAACAGAACACACGGGCGGAGTTTGAGGATGCTTTCCGAGCGAT CGATCATAACAAGGATGGAAAGGTCGACATAACAGAGCTCAATAGGCTCA TGGTTGCTACAGGGTTTGGACTGACCAACCAAGAGTTGAAAGAATTTTTT GGCTTCTTCGACCGTGACGGCAGTGGGAAAGTGGACATGGTAGAATTTTT GGACTATATGATGGACGCCTAA
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Coding sequence (CDS) from alignment at Ecto-sp13_S_contig1548:16015..16386-

>mRNA_Ecto-sp13_S_contig1548.4091.1 ID=mRNA_Ecto-sp13_S_contig1548.4091.1|Name=mRNA_Ecto-sp13_S_contig1548.4091.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=CDS|length=372bp|location=Sequence derived from alignment at Ecto-sp13_S_contig1548:16015..16386- (Ectocarpus species13 EcNAP12_S_4_19m)
GGGGCACCAGGCACGAAACGTTCGTGCAGGCCCGTATCCTCAACAACCAC
TATTTTGCTACGGGCTGGTCGGCGGCACAATCCACGTGCACTTCGTCGTT
CCCACTACTTTCGTCTCTCTCCCGCCGCTTCCCCATGGGTACCCAATACT
TGCGAACAACAGAACACACGGGCGGAGTTTGAGGATGCTTTCCGAGCGAT
CGATCATAACAAGGATGGAAAGGTCGACATAACAGAGCTCAATAGGCTCA
TGGTTGCTACAGGGTTTGGACTGACCAACCAAGAGTTGAAAGAATTTTTT
GGCTTCTTCGACCGTGACGGCAGTGGGAAAGTGGACATGGTAGAATTTTT
GGACTATATGATGGACGCCTAA
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