mRNA_Ecto-sp13_S_contig15165.3895.1 (mRNA) Ectocarpus species13 EcNAP12_S_4_19m

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_Ecto-sp13_S_contig15165.3895.1
Unique NamemRNA_Ecto-sp13_S_contig15165.3895.1
TypemRNA
OrganismEctocarpus species13 EcNAP12_S_4_19m (Ectocarpus species13 EcNAP12_S_4_19m)
Homology
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BLAST of mRNA_Ecto-sp13_S_contig15165.3895.1 vs. uniprot
Match: D7FU74_ECTSI (RanBD1 domain-containing protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FU74_ECTSI)

HSP 1 Score: 145 bits (366), Expect = 5.310e-43
Identity = 101/109 (92.66%), Postives = 101/109 (92.66%), Query Frame = 1 
Query:    1 MSDFAQLAAKAKEDGVAAAAAPVDTXXXXXXXXXXXXXXXXXXXXEEESTAQFEPVVQLEEVEVKTHEEDEEVLWKMRAKLFIFGETLLNKGTGQKEWVERGIGEVKFL 327
            MSDFAQLAAKAKEDGVAA       XXXXXXXXXXXXXXXXXXXX EESTAQFEPVVQLEEVEVKTHEEDEEVLWKMRAKLFIFGETLLNKGTGQKEWVERGIGEVKFL
Sbjct:    1 MSDFAQLAAKAKEDGVAAXXXXXXXXXXXXXXXXXXXXXXXXXXXXEESTAQFEPVVQLEEVEVKTHEEDEEVLWKMRAKLFIFGETLLNKGTGQKEWVERGIGEVKFL 109
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BLAST of mRNA_Ecto-sp13_S_contig15165.3895.1 vs. uniprot
Match: A0A6H5KI29_9PHAE (RanBD1 domain-containing protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5KI29_9PHAE)

HSP 1 Score: 137 bits (345), Expect = 3.930e-38
Identity = 97/110 (88.18%), Postives = 97/110 (88.18%), Query Frame = 1 
Query:    1 MSDFAQLAAKAKEDGVAAAAAPVDTXXXXXXXXXXXXXXXXXXXXEEESTAQFEPVVQLEEVEVKTHEEDEEVLWKMRAKLFIFGETLLNKGTGQKEWVERGIGEVKFLQ 330
            MSDFAQLAAKAKEDG          XXXXXXXXXXXXXXXXXXXX   STAQFEPVVQLEEVEVKTHEEDEEVLWKMRAKLFIFGETLLNKGTGQKEWVERGIGEVKFLQ
Sbjct:    1 MSDFAQLAAKAKEDGAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXSTAQFEPVVQLEEVEVKTHEEDEEVLWKMRAKLFIFGETLLNKGTGQKEWVERGIGEVKFLQ 110
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BLAST of mRNA_Ecto-sp13_S_contig15165.3895.1 vs. uniprot
Match: A0A7S2XZR9_9STRA (Hypothetical protein n=1 Tax=Fibrocapsa japonica TaxID=94617 RepID=A0A7S2XZR9_9STRA)

HSP 1 Score: 109 bits (272), Expect = 5.280e-27
Identity = 52/63 (82.54%), Postives = 57/63 (90.48%), Query Frame = 1 
Query:  142 ESTAQFEPVVQLEEVEVKTHEEDEEVLWKMRAKLFIFGETLLNKGTGQKEWVERGIGEVKFLQ 330
            ESTA FEPVVQL+E+EVKTHEE+EEVLWK R KLF+FGETLLNKGTGQK W ERGIGE KFL+
Sbjct:   68 ESTAHFEPVVQLDEIEVKTHEEEEEVLWKWRGKLFVFGETLLNKGTGQKTWNERGIGEFKFLK 130
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BLAST of mRNA_Ecto-sp13_S_contig15165.3895.1 vs. uniprot
Match: A0A835YXP9_9STRA (RanBP1 domain-containing protein n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835YXP9_9STRA)

HSP 1 Score: 104 bits (260), Expect = 1.270e-25
Identity = 51/65 (78.46%), Postives = 58/65 (89.23%), Query Frame = 1 
Query:  136 EEESTAQFEPVVQLEEVEVKTHEEDEEVLWKMRAKLFIFGETLLNKGTGQKEWVERGIGEVKFLQ 330
            E E T  FEPVVQL+EVEVKTHEEDE+VL+KMRAKLF+FGETLLNKG+G K W ERG+GEVKFL+
Sbjct:   42 EAECTTVFEPVVQLDEVEVKTHEEDEQVLYKMRAKLFVFGETLLNKGSGTKTWNERGVGEVKFLK 106
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BLAST of mRNA_Ecto-sp13_S_contig15165.3895.1 vs. uniprot
Match: A0A8J2T1Z3_9STRA (Hypothetical protein n=1 Tax=Pelagomonas calceolata TaxID=35677 RepID=A0A8J2T1Z3_9STRA)

HSP 1 Score: 100 bits (249), Expect = 1.430e-23
Identity = 48/64 (75.00%), Postives = 59/64 (92.19%), Query Frame = 1 
Query:  139 EESTAQFEPVVQLEEVEVKTHEEDEEVLWKMRAKLFIFGETLLNKGTGQKEWVERGIGEVKFLQ 330
            +ESTA+F PVVQL+EVEVKTHEEDE+V +K+RAKLF F ETLLNKG+G+KEW+ERG+GEVK L+
Sbjct:   93 QESTAEFTPVVQLDEVEVKTHEEDEDVDFKIRAKLFRFSETLLNKGSGKKEWIERGVGEVKILK 156
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BLAST of mRNA_Ecto-sp13_S_contig15165.3895.1 vs. uniprot
Match: A0A6S8ZTG3_9STRA (Hypothetical protein n=2 Tax=Ditylum brightwellii TaxID=49249 RepID=A0A6S8ZTG3_9STRA)

HSP 1 Score: 97.1 bits (240), Expect = 8.270e-23
Identity = 48/65 (73.85%), Postives = 57/65 (87.69%), Query Frame = 1 
Query:  136 EEESTAQFEPVVQLEEVEVKTHEEDEEVLWKMRAKLFIFGETLLNKGTGQKEWVERGIGEVKFLQ 330
            EEESTA F+PVV+LEEVEVK+ EEDEEV++ MRAKLF+FGETLL+KGTG K W ERGIGE + L+
Sbjct:   26 EEESTATFKPVVELEEVEVKSGEEDEEVVFSMRAKLFLFGETLLDKGTGNKSWRERGIGEARILR 90
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BLAST of mRNA_Ecto-sp13_S_contig15165.3895.1 vs. uniprot
Match: A0A7S3NPI9_9STRA (Hypothetical protein n=1 Tax=Aureoumbra lagunensis TaxID=44058 RepID=A0A7S3NPI9_9STRA)

HSP 1 Score: 97.1 bits (240), Expect = 2.680e-22
Identity = 46/65 (70.77%), Postives = 57/65 (87.69%), Query Frame = 1 
Query:  136 EEESTAQFEPVVQLEEVEVKTHEEDEEVLWKMRAKLFIFGETLLNKGTGQKEWVERGIGEVKFLQ 330
            E EST +F PVVQL+EVEVKT EEDEE+++KMRA+L+ F ETLLNKGTG+KEW+ERG+GE K L+
Sbjct:   79 EAESTQEFTPVVQLDEVEVKTLEEDEEIIFKMRARLYRFAETLLNKGTGKKEWIERGVGEAKLLK 143
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BLAST of mRNA_Ecto-sp13_S_contig15165.3895.1 vs. uniprot
Match: A0A7S0C0M0_9STRA (Hypothetical protein n=2 Tax=Proboscia inermis TaxID=420281 RepID=A0A7S0C0M0_9STRA)

HSP 1 Score: 94.7 bits (234), Expect = 7.280e-22
Identity = 46/65 (70.77%), Postives = 56/65 (86.15%), Query Frame = 1 
Query:  136 EEESTAQFEPVVQLEEVEVKTHEEDEEVLWKMRAKLFIFGETLLNKGTGQKEWVERGIGEVKFLQ 330
            EEESTA FEPVV+LEEVE K+ EEDEE+L  MR KLF++GETLL+KGTG+K W ERGIGE++ L+
Sbjct:   35 EEESTATFEPVVRLEEVETKSGEEDEEILHGMRCKLFVYGETLLDKGTGKKSWRERGIGEIRLLK 99
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BLAST of mRNA_Ecto-sp13_S_contig15165.3895.1 vs. uniprot
Match: A0A7R9ZPH3_9STRA (Hypothetical protein n=1 Tax=Craspedostauros australis TaxID=1486917 RepID=A0A7R9ZPH3_9STRA)

HSP 1 Score: 94.7 bits (234), Expect = 2.400e-21
Identity = 47/65 (72.31%), Postives = 57/65 (87.69%), Query Frame = 1 
Query:  136 EEESTAQFEPVVQLEEVEVKTHEEDEEVLWKMRAKLFIFGETLLNKGTGQKEWVERGIGEVKFLQ 330
            E+ESTAQFEPVV+LEEV+VK+ EEDEEV++ +R+KLFIFGETLL+ GTG K W ERGIGE K L+
Sbjct:   91 EDESTAQFEPVVKLEEVDVKSGEEDEEVVYGIRSKLFIFGETLLDVGTGNKSWKERGIGEAKILR 155
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BLAST of mRNA_Ecto-sp13_S_contig15165.3895.1 vs. uniprot
Match: A0A7S2EXM6_TRICV (Hypothetical protein n=2 Tax=Eupodiscales TaxID=33838 RepID=A0A7S2EXM6_TRICV)

HSP 1 Score: 92.8 bits (229), Expect = 3.400e-21
Identity = 46/65 (70.77%), Postives = 58/65 (89.23%), Query Frame = 1 
Query:  136 EEESTAQFEPVVQLEEVEVKTHEEDEEVLWKMRAKLFIFGETLLNKGTGQKEWVERGIGEVKFLQ 330
            EEESTA FEPVV+L+EVEV++ EEDEEV++ +R+KLFI+GETLL+KGTG+K W ERGIGE K L+
Sbjct:   22 EEESTATFEPVVKLDEVEVQSGEEDEEVVFGVRSKLFIYGETLLDKGTGKKTWRERGIGEAKILR 86
The following BLAST results are available for this feature:
BLAST of mRNA_Ecto-sp13_S_contig15165.3895.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
D7FU74_ECTSI5.310e-4392.66RanBD1 domain-containing protein n=1 Tax=Ectocarpu... [more]
A0A6H5KI29_9PHAE3.930e-3888.18RanBD1 domain-containing protein n=1 Tax=Ectocarpu... [more]
A0A7S2XZR9_9STRA5.280e-2782.54Hypothetical protein n=1 Tax=Fibrocapsa japonica T... [more]
A0A835YXP9_9STRA1.270e-2578.46RanBP1 domain-containing protein n=1 Tax=Tribonema... [more]
A0A8J2T1Z3_9STRA1.430e-2375.00Hypothetical protein n=1 Tax=Pelagomonas calceolat... [more]
A0A6S8ZTG3_9STRA8.270e-2373.85Hypothetical protein n=2 Tax=Ditylum brightwellii ... [more]
A0A7S3NPI9_9STRA2.680e-2270.77Hypothetical protein n=1 Tax=Aureoumbra lagunensis... [more]
A0A7S0C0M0_9STRA7.280e-2270.77Hypothetical protein n=2 Tax=Proboscia inermis Tax... [more]
A0A7R9ZPH3_9STRA2.400e-2172.31Hypothetical protein n=1 Tax=Craspedostauros austr... [more]
A0A7S2EXM6_TRICV3.400e-2170.77Hypothetical protein n=2 Tax=Eupodiscales TaxID=33... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
Ecto-sp13_S_contig15165contigEcto-sp13_S_contig15165:581..2414 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Ectocarpus species13 EcNAP12_S_4_19m OGS1.02022-07-08
Properties
Property NameValue
Taxonomic scopeEukaryota
Stop1
Start1
Seed ortholog score145.6
Seed ortholog evalue1.4e-32
Seed eggNOG ortholog2880.D7FU74
Model size333
KEGG koko:K12172,ko:K15306,ko:K20282
KEGG Pathwayko03013,ko05166,ko05203,map03013,map05166,map05203
KEGG ModuleM00427
Hectar predicted targeting categoryother localisation
GOsGO:0000082,GO:0000226,GO:0000278,GO:0003674,GO:0003824,GO:0005488,GO:0005515,GO:0005575,GO:0005622,GO:0005623,GO:0005634,GO:0005635,GO:0005642,GO:0005643,GO:0005737,GO:0005813,GO:0005815,GO:0005829,GO:0005856,GO:0006403,GO:0006405,GO:0006406,GO:0006464,GO:0006508,GO:0006511,GO:0006606,GO:0006607,GO:0006611,GO:0006807,GO:0006810,GO:0006886,GO:0006913,GO:0006996,GO:0007010,GO:0007017,GO:0007049,GO:0007051,GO:0007088,GO:0007154,GO:0007165,GO:0007346,GO:0008104,GO:0008150,GO:0008152,GO:0008284,GO:0008536,GO:0009056,GO:0009057,GO:0009719,GO:0009725,GO:0009755,GO:0009893,GO:0009987,GO:0010033,GO:0010467,GO:0010468,GO:0010564,GO:0010604,GO:0010628,GO:0010638,GO:0012505,GO:0015031,GO:0015630,GO:0015833,GO:0015931,GO:0016020,GO:0016032,GO:0016043,GO:0016234,GO:0016740,GO:0016925,GO:0017016,GO:0017038,GO:0018193,GO:0018205,GO:0019222,GO:0019538,GO:0019787,GO:0019789,GO:0019899,GO:0019941,GO:0022402,GO:0023052,GO:0030163,GO:0030522,GO:0031090,GO:0031267,GO:0031503,GO:0031965,GO:0031967,GO:0031975,GO:0032239,GO:0032241,GO:0032386,GO:0032388,GO:0032446,GO:0032870,GO:0032879,GO:0032880,GO:0032886,GO:0032991,GO:0033036,GO:0033043,GO:0033365,GO:0034504,GO:0034613,GO:0035626,GO:0036211,GO:0042127,GO:0042175,GO:0042221,GO:0042405,GO:0042886,GO:0043170,GO:0043226,GO:0043227,GO:0043228,GO:0043229,GO:0043231,GO:0043232,GO:0043412,GO:0043632,GO:0044237,GO:0044238,GO:0044248,GO:0044257,GO:0044260,GO:0044265,GO:0044267,GO:0044403,GO:0044419,GO:0044422,GO:0044424,GO:0044425,GO:0044428,GO:0044430,GO:0044444,GO:0044446,GO:0044464,GO:0044614,GO:0044615,GO:0044770,GO:0044772,GO:0044843,GO:0045184,GO:0045787,GO:0045840,GO:0045931,GO:0046602,GO:0046604,GO:0046605,GO:0046607,GO:0046822,GO:0046824,GO:0046831,GO:0046833,GO:0046907,GO:0048518,GO:0048522,GO:0050657,GO:0050658,GO:0050789,GO:0050794,GO:0050896,GO:0051020,GO:0051028,GO:0051049,GO:0051050,GO:0051128,GO:0051130,GO:0051168,GO:0051169,GO:0051170,GO:0051179,GO:0051234,GO:0051236,GO:0051493,GO:0051495,GO:0051603,GO:0051640,GO:0051641,GO:0051642,GO:0051649,GO:0051704,GO:0051716,GO:0051726,GO:0051783,GO:0051785,GO:0060255,GO:0060341,GO:0061842,GO:0065007,GO:0070507,GO:0070647,GO:0070727,GO:0070887,GO:0071166,GO:0071310,GO:0071426,GO:0071427,GO:0071495,GO:0071702,GO:0071704,GO:0071705,GO:0071840,GO:0072594,GO:0090068,GO:0098589,GO:0098805,GO:0140096,GO:1900180,GO:1900182,GO:1901564,GO:1901565,GO:1901575,GO:1903047,GO:1903827,GO:1903829
Exons2
EggNOG free text desc.Ran GTPase binding
EggNOG OGsCOG5171@1,KOG0864@2759
Ec32 ortholog descriptionRan binding domain
Ec32 orthologEc-25_003000.1
Cds size333
COG Functional cat.U
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko00002,ko03019,ko03110,ko04121,ko04131
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1681462806.9170651-CDS-Ecto-sp13_S_contig15165:580..8101681462806.9170651-CDS-Ecto-sp13_S_contig15165:580..810Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig15165 581..810 +
1681462806.9529183-CDS-Ecto-sp13_S_contig15165:2311..24141681462806.9529183-CDS-Ecto-sp13_S_contig15165:2311..2414Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig15165 2312..2414 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_Ecto-sp13_S_contig15165.3895.1prot_Ecto-sp13_S_contig15165.3895.1Ectocarpus species13 EcNAP12_S_4_19mpolypeptideEcto-sp13_S_contig15165 581..2414 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_Ecto-sp13_S_contig15165.3895.1

>prot_Ecto-sp13_S_contig15165.3895.1 ID=prot_Ecto-sp13_S_contig15165.3895.1|Name=mRNA_Ecto-sp13_S_contig15165.3895.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=polypeptide|length=111bp
MSDFAQLAAKAKEDGVAAAAAPVDTTSETAAPASAAVDEGAGAAAEEEST
AQFEPVVQLEEVEVKTHEEDEEVLWKMRAKLFIFGETLLNKGTGQKEWVE
RGIGEVKFLQ*
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mRNA from alignment at Ecto-sp13_S_contig15165:581..2414+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_Ecto-sp13_S_contig15165.3895.1 ID=mRNA_Ecto-sp13_S_contig15165.3895.1|Name=mRNA_Ecto-sp13_S_contig15165.3895.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=mRNA|length=1834bp|location=Sequence derived from alignment at Ecto-sp13_S_contig15165:581..2414+ (Ectocarpus species13 EcNAP12_S_4_19m)
ATGTCTGACTTCGCTCAGCTCGCGGCCAAGGCCAAGGAGGATGGAGTGGC
CGCCGCCGCCGCGCCTGTCGACACCACGAGCGAGACGGCCGCGCCCGCCT
CTGCAGCAGTAGACGAGGGAGCAGGGGCAGCGGCTGAGGAGGAGAGCACT
GCGCAGTTCGAACCCGTGGTTCAGCTGGAGGAGGTGGAGGTGAAGACACA
CGAAGAGGACGAGGAAGTTCTGTGGAAGATGTGAGTGTTGGTTGTTTTGG
GGCGCGACGACGACGACCCACGGGAAATGGAGGGAGGGAAAAAGGGAACA
AGCAATCTCCCGCGCCAAATTTTTGCTTGGTCCCGAAGCACGCCAGCCAG
CTCCTTTGCTGCTGGCCGTCGCTGAATCAGATTGACTGCAGCAGCGACAT
GGAAGAGTTCCGTAGTCAATCGTGCATGCCACACGCATTTGGTGTGTGTT
TGGCGCGCGTAGTGTGTGGCAGGACAACCATGACTGGGGGCGCACCTTCT
CTCCGTTGAAATTTATTCGTAGCACTGTGAGGTCTTTACAGCAGTATCCG
CCATCTCGAAGCCCGGCGTGGCAGCGAAGTGACCTTATTGTGCAGCAGCA
GCAGCTCAACTAGTCTACTCCCCAGCGTTGTGGTCGAACGGGTCTTGTAG
AGAAATCTTCAGCCCCTAGGTGAGAAACCAAAGCAATACTGGTGTGCGGT
GTGCATACCTTGTGTGTTTTTCGGCCGCAAGAGCACTCTGTGCGGCTGGA
TTTGAATCGCACAAGAAAGAGTGTCTGCTGTCGATCTCGATCGACCCTCC
CTCACCACACTCGGCGATCCTCTGCTTTTACACTTGGCGATCCACTGCTG
TGCATTGGCTGGGGCAGGGGCGGGGGCGGCAGGATGTCTCTCGTGCGCTT
GCTCAAATCTTTTATGCGGCACAGGAGATCCTCTGTCCCCTTGTAAATAC
AACCGCATGGGGATCAGGGCTACGCAGTAGCCTTTCTTTAGAATGAAGGC
CCTCGTTCTTGGTGTCCCCTCTAGGTGAGGAGGCAGTTTCGTTCAGGGCG
GGTCGCTGTTGCCCGTACGCCGATGATGATTCGGGGTGGAGCCGTGGTGC
CGCGCAATTTGTGGTCACTGCACATGGGCATGTCGTAAAACCAGGACCAT
TATCCTCTGTATTATGGTGTAAACGTGACGACGCGCTGCGGCCAACCGGG
CTACGGCCGCATCTATCGTGTGATGTTACCCCGTCCACGCTAGGTCGATC
ACGTGAGGGTTGTGTCTTGGACCTGTGGATCTCCAAGGACGACTGTAAAT
ATGCATGAACCAATCATGAAGCTTGCAAACGTCGAACATGATGCACGAAG
CAGCAGCAGCGGTAGCCACTACAGCCCCTCCCCCCCGTCTTGCCGCGTTT
TCATCCTCCGCTCTGACGGTCAATTTGCATGTGTTTCGGCTGCTGACCTC
TCACGGGAGCTTCGTCTTCTGATATTTACTTGTTGTGTTCTATGCTTGCT
AATTTTCCAAGAACATAGGCCTGAACCCTGTCTCGTACGGACGATCTGCT
TCGATGTTGGGCGTCCTCTCAATAAATATGCTCGCGTCGCTGCTGCCGAC
ATTTCTCACTTACCCCTTTCGTCTCCTCCCCGCAATACTTGCCCCGTCAC
TACCTGTACCTTCTTGTGAACGTCGGGTGTGTCTTAACTCTCGTCCGGTC
ATCCCTGTCTGTGGTGATCCCTCACCTCCAGGCGCGCTAAGCTCTTCATT
TTTGGCGAGACACTGCTTAACAAGGGGACTGGGCAGAAGGAGTGGGTGGA
GAGAGGCATCGGCGAGGTCAAGTTCCTCCAGTGA
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Coding sequence (CDS) from alignment at Ecto-sp13_S_contig15165:581..2414+

>mRNA_Ecto-sp13_S_contig15165.3895.1 ID=mRNA_Ecto-sp13_S_contig15165.3895.1|Name=mRNA_Ecto-sp13_S_contig15165.3895.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=CDS|length=333bp|location=Sequence derived from alignment at Ecto-sp13_S_contig15165:581..2414+ (Ectocarpus species13 EcNAP12_S_4_19m)
ATGTCTGACTTCGCTCAGCTCGCGGCCAAGGCCAAGGAGGATGGAGTGGC
CGCCGCCGCCGCGCCTGTCGACACCACGAGCGAGACGGCCGCGCCCGCCT
CTGCAGCAGTAGACGAGGGAGCAGGGGCAGCGGCTGAGGAGGAGAGCACT
GCGCAGTTCGAACCCGTGGTTCAGCTGGAGGAGGTGGAGGTGAAGACACA
CGAAGAGGACGAGGAAGTTCTGTGGAAGATGCGCGCTAAGCTCTTCATTT
TTGGCGAGACACTGCTTAACAAGGGGACTGGGCAGAAGGAGTGGGTGGAG
AGAGGCATCGGCGAGGTCAAGTTCCTCCAGTGA
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