mRNA_Ecto-sp13_S_contig12961.2430.1 (mRNA) Ectocarpus species13 EcNAP12_S_4_19m

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_Ecto-sp13_S_contig12961.2430.1
Unique NamemRNA_Ecto-sp13_S_contig12961.2430.1
TypemRNA
OrganismEctocarpus species13 EcNAP12_S_4_19m (Ectocarpus species13 EcNAP12_S_4_19m)
Homology
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BLAST of mRNA_Ecto-sp13_S_contig12961.2430.1 vs. uniprot
Match: D8LUD2_ECTSI (DRMBL domain-containing protein n=2 Tax=Ectocarpus TaxID=2879 RepID=D8LUD2_ECTSI)

HSP 1 Score: 576 bits (1485), Expect = 3.160e-196
Identity = 305/330 (92.42%), Postives = 310/330 (93.94%), Query Frame = 2 
Query:    2 YSEHSSFTQLKDFVRTVRPKKIVPTVNAVSSASVEKMLSHFLEFMDLSSDRKRLESYFCKRDPPSATASRGIDMLSSPGARTSNPLSSPAEKRRKNADSSIGWPPNLFPAEKPSRPPLKAAERNRLHENDATSGTTMDDCPCVPRREGEALRSSAGEQPCGDAGPLPEATRTAEAAVPAAMPAGSVVAGAGTGASSTPSAVELDLVDVEAQKAIMADIERRKRGQQETKTNGAHTSQSGGNSRERRRKSTPGSGRKRTNSSTRSDDGRPADASLRGFFGGNVVSDAKGTRALAESVGGCRGQEVLTIDDEDCGTETPEPMDIRDFFSRRK 991
            YSEHSSFTQLKDFVRTVRPKKIVPTVNAVSSASVEKMLSHFLEFMDLSSDRKRLESYFCKRDPPSATASRGIDMLSS GAR SNP SSPAEKRRKNA+SS GWPPNLFPAEKPSRPPLKAAERNRLHENDATSGTT+DD PCVPRREGEALRS AGEQPCGDAG LPEAT TAEAAVPAAMPA S VA AG  A+STPSAVELDLVDVEAQKAIMADIERRKRGQQETK  GAHT+QSGGNSRE RRKSTPGSGRKRTNSSTRSDDGRPADA+LRGFFGG VVSDAKGTR LAESVGGCRG EVLTIDDEDCGTE PEPMDIRDFFSRRK
Sbjct:  382 YSEHSSFTQLKDFVRTVRPKKIVPTVNAVSSASVEKMLSHFLEFMDLSSDRKRLESYFCKRDPPSATASRGIDMLSSSGARASNPFSSPAEKRRKNANSSTGWPPNLFPAEKPSRPPLKAAERNRLHENDATSGTTLDDYPCVPRREGEALRSRAGEQPCGDAGRLPEATMTAEAAVPAAMPARSFVADAGARAASTPSAVELDLVDVEAQKAIMADIERRKRGQQETKATGAHTAQSGGNSRESRRKSTPGSGRKRTNSSTRSDDGRPADANLRGFFGGKVVSDAKGTRTLAESVGGCRGHEVLTIDDEDCGTELPEPMDIRDFFSRRK 711
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BLAST of mRNA_Ecto-sp13_S_contig12961.2430.1 vs. uniprot
Match: A0A7S0PPJ7_MICPS (Hypothetical protein (Fragment) n=1 Tax=Micromonas pusilla TaxID=38833 RepID=A0A7S0PPJ7_MICPS)

HSP 1 Score: 75.9 bits (185), Expect = 2.630e-12
Identity = 37/81 (45.68%), Postives = 55/81 (67.90%), Query Frame = 2 
Query:    2 YSEHSSFTQLKDFVRTVRPKKIVPTVNAVSSASVEKMLSHFLEFMDLSSDRKRLESYFC-KRDPPSATASRGIDMLSSPGA 241
            YSEHSSF +L +FV+ +RP+++ PTVNA + A  ++++ HFLEF DL++D+ RL+ YF  KR  P   A    ++ S P A
Sbjct:   60 YSEHSSFVELVEFVKRIRPRRVTPTVNADTKADKDRIMKHFLEFTDLAADKNRLDHYFFNKRSAPEVKAEVKREIESDPVA 140
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BLAST of mRNA_Ecto-sp13_S_contig12961.2430.1 vs. uniprot
Match: K8YRT5_NANGC (Hydrolase involved in interstrand cross-link repair n=1 Tax=Nannochloropsis gaditana (strain CCMP526) TaxID=1093141 RepID=K8YRT5_NANGC)

HSP 1 Score: 74.3 bits (181), Expect = 2.270e-11
Identity = 34/57 (59.65%), Postives = 42/57 (73.68%), Query Frame = 2 
Query:    2 YSEHSSFTQLKDFVRTVRPKKIVPTVNAVSSASVEKMLSHFLEFMDLSSDRKRLESY 172
            YSEHSS+T+L+ FVR +RP+KI+PTV A     VE+ L HFL  MDLS DR RL+ Y
Sbjct:   74 YSEHSSYTELRSFVRAIRPRKIIPTVGAGDEKGVERQLKHFLADMDLSHDRSRLDRY 130
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BLAST of mRNA_Ecto-sp13_S_contig12961.2430.1 vs. uniprot
Match: W7THR8_9STRA (Dna cross-link repair 1a protein n=1 Tax=Nannochloropsis gaditana TaxID=72520 RepID=W7THR8_9STRA)

HSP 1 Score: 74.3 bits (181), Expect = 1.600e-10
Identity = 34/57 (59.65%), Postives = 42/57 (73.68%), Query Frame = 2 
Query:    2 YSEHSSFTQLKDFVRTVRPKKIVPTVNAVSSASVEKMLSHFLEFMDLSSDRKRLESY 172
            YSEHSS+T+L+ FVR +RP+KI+PTV A     VE+ L HFL  MDLS DR RL+ Y
Sbjct:  243 YSEHSSYTELRSFVRAIRPRKIIPTVGAGDEKGVERQLKHFLADMDLSHDRSRLDRY 299
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BLAST of mRNA_Ecto-sp13_S_contig12961.2430.1 vs. uniprot
Match: A0A4D9CSH8_9STRA (DRMBL domain-containing protein n=1 Tax=Nannochloropsis salina CCMP1776 TaxID=1027361 RepID=A0A4D9CSH8_9STRA)

HSP 1 Score: 73.2 bits (178), Expect = 6.610e-10
Identity = 33/57 (57.89%), Postives = 42/57 (73.68%), Query Frame = 2 
Query:    2 YSEHSSFTQLKDFVRTVRPKKIVPTVNAVSSASVEKMLSHFLEFMDLSSDRKRLESY 172
            YSEHSS+T+L+ FVR +RP+KI+PTV A     VE+ L HFL  MDLS D+ RL+ Y
Sbjct:  402 YSEHSSYTELRSFVRAIRPRKIIPTVGAGDEKGVERQLKHFLADMDLSHDKSRLDRY 458
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BLAST of mRNA_Ecto-sp13_S_contig12961.2430.1 vs. uniprot
Match: A0A8J9WR85_9CHLO (Probable DNA cross-link repair 1A protein at N-terminal half n=1 Tax=Coccomyxa sp. Obi TaxID=2315456 RepID=A0A8J9WR85_9CHLO)

HSP 1 Score: 72.4 bits (176), Expect = 1.580e-9
Identity = 36/70 (51.43%), Postives = 50/70 (71.43%), Query Frame = 2 
Query:    2 YSEHSSFTQLKDFVRTVRPKKIVPTVNAVSSASVEKMLSHFLEFMDLSSDRKRLESYFCK-RDPPSATAS 208
            YSEHSSF +L+D V+T+RP++IVPTVNA + A+   ++  F + MDLS DR RL+ +  K +  P ATAS
Sbjct:  298 YSEHSSFAELRDCVKTLRPRRIVPTVNASTPAAARSLVDRFADLMDLSKDRSRLDFFLRKAKTAPPATAS 367
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BLAST of mRNA_Ecto-sp13_S_contig12961.2430.1 vs. uniprot
Match: A0A5J4Y9L3_9CHLO (DNA cross-link repair SNM1-like n=1 Tax=Trebouxia sp. A1-2 TaxID=2608996 RepID=A0A5J4Y9L3_9CHLO)

HSP 1 Score: 70.9 bits (172), Expect = 4.360e-9
Identity = 36/75 (48.00%), Postives = 49/75 (65.33%), Query Frame = 2 
Query:    2 YSEHSSFTQLKDFVRTVRPKKIVPTVNAVSSASVEKMLSHFLEFMDLSSDRKRLESYFCKRDPPSATASRGIDML 226
            YSEHSSF +L+D V+ +RPK+I+PTVN   +AS   ++  F + MDLS D+ RL+SYF +      TAS   D L
Sbjct:  352 YSEHSSFAELRDCVKRLRPKRIIPTVNCPDAASARAIVDRFADLMDLSRDKSRLDSYFGR------TASAPADAL 420
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BLAST of mRNA_Ecto-sp13_S_contig12961.2430.1 vs. uniprot
Match: A0A7R9T7F0_9CHLO (Hypothetical protein (Fragment) n=1 Tax=Ostreococcus sp. 'lucimarinus' TaxID=242159 RepID=A0A7R9T7F0_9CHLO)

HSP 1 Score: 67.8 bits (164), Expect = 3.360e-8
Identity = 30/59 (50.85%), Postives = 46/59 (77.97%), Query Frame = 2 
Query:    2 YSEHSSFTQLKDFVRTVRPKKIVPTVNAVSSASVEKMLS-HFLEFMDLSSDRKRLESYF 175
            YSEHSS+T+L++F++ ++PKKI PTVNA++    EK+++ HFL+ +DL  D  RL+ YF
Sbjct:  345 YSEHSSYTELREFIKFLKPKKITPTVNAITPKDREKLVNKHFLDLVDLKQDVNRLDYYF 403
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BLAST of mRNA_Ecto-sp13_S_contig12961.2430.1 vs. uniprot
Match: A0A2P6TNP6_CHLSO (DNA cross-link repair SNM1 isoform X1 n=1 Tax=Chlorella sorokiniana TaxID=3076 RepID=A0A2P6TNP6_CHLSO)

HSP 1 Score: 66.6 bits (161), Expect = 9.760e-8
Identity = 31/60 (51.67%), Postives = 41/60 (68.33%), Query Frame = 2 
Query:    2 YSEHSSFTQLKDFVRTVRPKKIVPTVNAVSSASVEKMLSHFLEFMDLSSDRKRLESYFCK 181
            YSEHSSF +L+D VR +RP++I+PTVNA   AS    +  F + MDLS+DR RL  Y  +
Sbjct:  724 YSEHSSFPELRDCVRALRPRRIIPTVNAGDPASSRAQVDRFADLMDLSADRSRLSYYLLR 783
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BLAST of mRNA_Ecto-sp13_S_contig12961.2430.1 vs. uniprot
Match: A0A383V4K8_TETOB (Lactamase_B domain-containing protein n=1 Tax=Tetradesmus obliquus TaxID=3088 RepID=A0A383V4K8_TETOB)

HSP 1 Score: 65.9 bits (159), Expect = 1.300e-7
Identity = 32/74 (43.24%), Postives = 51/74 (68.92%), Query Frame = 2 
Query:    2 YSEHSSFTQLKDFVRTVRPKKIVPTVNAVSSASVEKMLSHFLEFMDLSSDRKRLESYFCKRDPPSATASRGIDM 223
            YSEHSS+ +L+  V  +RP K++PTVNA S A    ++  F++ MDLSS+R RL+ +  +R  P+A++S+G  +
Sbjct:  297 YSEHSSWDELRQCVAALRPSKLIPTVNAGSKAKSAALVERFVDLMDLSSNRGRLDMFLVRR--PAASSSQGAQL 368
The following BLAST results are available for this feature:
BLAST of mRNA_Ecto-sp13_S_contig12961.2430.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
D8LUD2_ECTSI3.160e-19692.42DRMBL domain-containing protein n=2 Tax=Ectocarpus... [more]
A0A7S0PPJ7_MICPS2.630e-1245.68Hypothetical protein (Fragment) n=1 Tax=Micromonas... [more]
K8YRT5_NANGC2.270e-1159.65Hydrolase involved in interstrand cross-link repai... [more]
W7THR8_9STRA1.600e-1059.65Dna cross-link repair 1a protein n=1 Tax=Nannochlo... [more]
A0A4D9CSH8_9STRA6.610e-1057.89DRMBL domain-containing protein n=1 Tax=Nannochlor... [more]
A0A8J9WR85_9CHLO1.580e-951.43Probable DNA cross-link repair 1A protein at N-ter... [more]
A0A5J4Y9L3_9CHLO4.360e-948.00DNA cross-link repair SNM1-like n=1 Tax=Trebouxia ... [more]
A0A7R9T7F0_9CHLO3.360e-850.85Hypothetical protein (Fragment) n=1 Tax=Ostreococc... [more]
A0A2P6TNP6_CHLSO9.760e-851.67DNA cross-link repair SNM1 isoform X1 n=1 Tax=Chlo... [more]
A0A383V4K8_TETOB1.300e-743.24Lactamase_B domain-containing protein n=1 Tax=Tetr... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
Ecto-sp13_S_contig12961contigEcto-sp13_S_contig12961:2..2068 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Ectocarpus species13 EcNAP12_S_4_19m OGS1.02022-07-08
Properties
Property NameValue
Taxonomic scopeEukaryota
Stop1
Start1
Seed ortholog score514.2
Seed ortholog evalue4e-143
Seed eggNOG ortholog2880.D8LUD2
Model size1800
KEGG koko:K15340
Hectar predicted targeting categoryother localisation
Exons2
EggNOG free text desc.protection from non-homologous end joining at telomere
EggNOG OGsCOG1236@1,KOG1361@2759
Ec32 ortholog descriptionBeta-lactamase-like
Ec32 orthologEc-14_004530.1
Cds size885
COG Functional cat.J
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko03400
Relationships

The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1681462693.4768772-UTR-Ecto-sp13_S_contig12961:1..611681462693.4768772-UTR-Ecto-sp13_S_contig12961:1..61Ectocarpus species13 EcNAP12_S_4_19mUTREcto-sp13_S_contig12961 2..61 +
1681462693.4875855-UTR-Ecto-sp13_S_contig12961:328..3771681462693.4875855-UTR-Ecto-sp13_S_contig12961:328..377Ectocarpus species13 EcNAP12_S_4_19mUTREcto-sp13_S_contig12961 329..377 +
1681462693.5065348-UTR-Ecto-sp13_S_contig12961:1262..20681681462693.5065348-UTR-Ecto-sp13_S_contig12961:1262..2068Ectocarpus species13 EcNAP12_S_4_19mUTREcto-sp13_S_contig12961 1263..2068 +


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1681462693.4958808-CDS-Ecto-sp13_S_contig12961:377..12621681462693.4958808-CDS-Ecto-sp13_S_contig12961:377..1262Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig12961 378..1262 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_Ecto-sp13_S_contig12961.2430.1prot_Ecto-sp13_S_contig12961.2430.1Ectocarpus species13 EcNAP12_S_4_19mpolypeptideEcto-sp13_S_contig12961 378..1262 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_Ecto-sp13_S_contig12961.2430.1

>prot_Ecto-sp13_S_contig12961.2430.1 ID=prot_Ecto-sp13_S_contig12961.2430.1|Name=mRNA_Ecto-sp13_S_contig12961.2430.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=polypeptide|length=295bp
MLSHFLEFMDLSSDRKRLESYFCKRDPPSATASRGIDMLSSPGARTSNPL
SSPAEKRRKNADSSIGWPPNLFPAEKPSRPPLKAAERNRLHENDATSGTT
MDDCPCVPRREGEALRSSAGEQPCGDAGPLPEATRTAEAAVPAAMPAGSV
VAGAGTGASSTPSAVELDLVDVEAQKAIMADIERRKRGQQETKTNGAHTS
QSGGNSRERRRKSTPGSGRKRTNSSTRSDDGRPADASLRGFFGGNVVSDA
KGTRALAESVGGCRGQEVLTIDDEDCGTETPEPMDIRDFFSRRK*
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mRNA from alignment at Ecto-sp13_S_contig12961:2..2068+

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_Ecto-sp13_S_contig12961.2430.1 ID=mRNA_Ecto-sp13_S_contig12961.2430.1|Name=mRNA_Ecto-sp13_S_contig12961.2430.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=mRNA|length=2067bp|location=Sequence derived from alignment at Ecto-sp13_S_contig12961:2..2068+ (Ectocarpus species13 EcNAP12_S_4_19m)
GTACTCGGAGCACAGCTCCTTCACCCAGCTGAAGGATTTTGTGCGAACCG
TTAGGCCAAAGTGAGTGGGCATCGTGTGGTGGGCGGTAGGGTCCGTCTCT
AGGGTATCTTGAAGCCTGTGTATGTTTTCAGTTCACCTGATGGTGAGGGC
TGATAACAACGGCGGTCACTGCGTCTCTGTAGTGTTTTTCTTGTTTCAAT
TTTTTTTGCTTGGATAGTCATCGTGTTCGGCGCTTGTTTCAAGTTATTTG
TTGCCGCTTCGTTCACTCATCTGATCGGTATCCCATGAAAGCGTCATTCG
TTGACTCGCCTAGTTCGTGTCGCGCAGGAAAATCGTCCCTACTGTCAATG
CAGTTTCTTCGGCCTCGGTGGAGAAGATGCTCAGCCATTTTTTGGAATTC
ATGGACCTATCCTCGGACCGAAAGCGCCTAGAGTCGTACTTTTGCAAGCG
TGATCCTCCCAGTGCGACCGCTTCTCGAGGCATCGATATGCTTTCTTCTC
CCGGCGCTAGGACGTCCAATCCGTTGAGTTCCCCAGCTGAAAAGCGACGA
AAGAACGCAGACAGCTCTATCGGGTGGCCGCCGAACCTCTTCCCCGCCGA
AAAACCTTCGCGCCCACCGTTGAAGGCAGCAGAAAGAAATCGTCTGCACG
AGAACGACGCGACATCTGGTACTACCATGGACGACTGTCCATGCGTCCCG
AGAAGAGAAGGGGAGGCCCTGCGTTCGAGTGCGGGGGAGCAGCCTTGCGG
TGATGCGGGACCTCTTCCGGAAGCGACAAGGACCGCCGAGGCGGCAGTGC
CGGCGGCGATGCCTGCCGGGTCTGTTGTAGCTGGTGCCGGCACGGGTGCC
TCTTCAACGCCCTCCGCCGTTGAGCTGGATCTTGTTGACGTGGAAGCACA
AAAGGCCATCATGGCTGACATCGAGCGCCGAAAGCGCGGGCAGCAGGAAA
CAAAGACAAACGGGGCCCACACTTCGCAGTCCGGAGGCAACTCGCGGGAG
AGACGTAGAAAAAGTACGCCTGGGTCCGGACGGAAACGAACCAACTCTAG
CACCAGAAGCGACGATGGTCGCCCGGCGGACGCTAGCTTGCGAGGGTTTT
TCGGCGGCAACGTCGTTTCGGATGCGAAGGGGACGAGAGCGCTTGCGGAG
TCGGTGGGTGGATGCAGAGGCCAGGAGGTGTTGACGATAGACGACGAGGA
CTGTGGAACGGAAACGCCGGAACCCATGGATATTCGCGACTTTTTCTCGC
GGCGTAAATAAGGCTGGCGGGCACATCGGTAGACTCGTAGGGAATGGCGA
GGGATATGGTCGGTGGACGAGGCCTCGCGGGCGATAGAAACGGATGCCGG
TTTTCGTCTTTGTCACGACTGCAGTCTCGACCGTCTACTCGACAGTCTTC
TTCCCGGTTTGACGACTTAAGCTCCCTTCCATAGCCGGTGTCCACGGATT
GTCCCCTTTCGGGGGGTTCGTCGATTGCCGTTCTGAGATGTCTTTTGGAT
TGCTGCAAAGCGGATCTCCCGTGTCTTGTTGCAGCTATAGTAAAGGCGTG
GGCGACCTCTTGAAGAAGGCACAGTGCTGTCACAACTTTGAGCTTTTTGT
AAGCGCGCGAGGCCGGCAATAAGGTGCACGTGGTGTCGCACCTGATATTC
CCACCCGTTGACAGCGTGGGAGCATCGTTGACATCGAACATTGTGACGTC
GACCGTCGTTCGTTGAAGTCAAACATCGTTGATTGATGTCGAGTAATGCT
GATTTCGATAATCGTTGACGTCACGCATCGTTGAAGCCGAGCATCGTTGG
CATCGAGCGTCGTTGACGTCCAGCTTCATAAGTTAACATCGAGGATCGTT
GATGTCGAGGATCGTTGATATGGAGGCATCGTTGGTGTCGAACATCGTTG
ATGTCGAGTATCGTTCAAGTCGGGGCATTGTTGACATCGAGTATTGTTGA
CGTCCAGCATCATTCTGACGTCAAGCATCGCTGACATCGACCATCGTTGA
CGCTGACCGCCGTTGGTATCGAGCATCGTTGATTGATGTCGAGCATCTTT
GATGTCGAGCATCATTG
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Coding sequence (CDS) from alignment at Ecto-sp13_S_contig12961:2..2068+

>mRNA_Ecto-sp13_S_contig12961.2430.1 ID=mRNA_Ecto-sp13_S_contig12961.2430.1|Name=mRNA_Ecto-sp13_S_contig12961.2430.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=CDS|length=885bp|location=Sequence derived from alignment at Ecto-sp13_S_contig12961:2..2068+ (Ectocarpus species13 EcNAP12_S_4_19m)
ATGCTCAGCCATTTTTTGGAATTCATGGACCTATCCTCGGACCGAAAGCG
CCTAGAGTCGTACTTTTGCAAGCGTGATCCTCCCAGTGCGACCGCTTCTC
GAGGCATCGATATGCTTTCTTCTCCCGGCGCTAGGACGTCCAATCCGTTG
AGTTCCCCAGCTGAAAAGCGACGAAAGAACGCAGACAGCTCTATCGGGTG
GCCGCCGAACCTCTTCCCCGCCGAAAAACCTTCGCGCCCACCGTTGAAGG
CAGCAGAAAGAAATCGTCTGCACGAGAACGACGCGACATCTGGTACTACC
ATGGACGACTGTCCATGCGTCCCGAGAAGAGAAGGGGAGGCCCTGCGTTC
GAGTGCGGGGGAGCAGCCTTGCGGTGATGCGGGACCTCTTCCGGAAGCGA
CAAGGACCGCCGAGGCGGCAGTGCCGGCGGCGATGCCTGCCGGGTCTGTT
GTAGCTGGTGCCGGCACGGGTGCCTCTTCAACGCCCTCCGCCGTTGAGCT
GGATCTTGTTGACGTGGAAGCACAAAAGGCCATCATGGCTGACATCGAGC
GCCGAAAGCGCGGGCAGCAGGAAACAAAGACAAACGGGGCCCACACTTCG
CAGTCCGGAGGCAACTCGCGGGAGAGACGTAGAAAAAGTACGCCTGGGTC
CGGACGGAAACGAACCAACTCTAGCACCAGAAGCGACGATGGTCGCCCGG
CGGACGCTAGCTTGCGAGGGTTTTTCGGCGGCAACGTCGTTTCGGATGCG
AAGGGGACGAGAGCGCTTGCGGAGTCGGTGGGTGGATGCAGAGGCCAGGA
GGTGTTGACGATAGACGACGAGGACTGTGGAACGGAAACGCCGGAACCCA
TGGATATTCGCGACTTTTTCTCGCGGCGTAAATAA
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