mRNA_Ecto-sp13_S_contig12756.2284.1 (mRNA) Ectocarpus species13 EcNAP12_S_4_19m

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_Ecto-sp13_S_contig12756.2284.1
Unique NamemRNA_Ecto-sp13_S_contig12756.2284.1
TypemRNA
OrganismEctocarpus species13 EcNAP12_S_4_19m (Ectocarpus species13 EcNAP12_S_4_19m)
Homology
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BLAST of mRNA_Ecto-sp13_S_contig12756.2284.1 vs. uniprot
Match: D7FL73_ECTSI (Trehalose 6-phosphate synthase, family GT20 / Trehalose 6-phosphate phosphatase n=2 Tax=Ectocarpus TaxID=2879 RepID=D7FL73_ECTSI)

HSP 1 Score: 238 bits (608), Expect = 3.790e-70
Identity = 117/120 (97.50%), Postives = 118/120 (98.33%), Query Frame = 1 
Query:   97 MANDASSSIMPTRPPPAYELSFAVEAKLGHGEVIYVCGSCPSLGAKDPSRALRLVTDPSSYPVWSSAPVPVPLNKKVSYRYCVVAGGKLKRWEDISVDRELDPVIEPSAATAIVEDVLDK 456
            MANDASSSIMPTRPPPAYELSFAVEAKLGHGEVIYVCGSCPSLGAKDPSRALRLVTDPSSYPVWSS PVPVPLNKKV+YRYCVVAGGKLKRWEDISVDRELDPV EPSAATAIVEDVLDK
Sbjct:    1 MANDASSSIMPTRPPPAYELSFAVEAKLGHGEVIYVCGSCPSLGAKDPSRALRLVTDPSSYPVWSSGPVPVPLNKKVTYRYCVVAGGKLKRWEDISVDRELDPVTEPSAATAIVEDVLDK 120
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BLAST of mRNA_Ecto-sp13_S_contig12756.2284.1 vs. uniprot
Match: V5K3Y9_SACJA (Trehalose-6-phosohate synthase n=1 Tax=Saccharina japonica TaxID=88149 RepID=V5K3Y9_SACJA)

HSP 1 Score: 195 bits (496), Expect = 5.850e-55
Identity = 93/108 (86.11%), Postives = 99/108 (91.67%), Query Frame = 1 
Query:  133 RPPPAYELSFAVEAKLGHGEVIYVCGSCPSLGAKDPSRALRLVTDPSSYPVWSSAPVPVPLNKKVSYRYCVVAGGKLKRWEDISVDRELDPVIEPSAATAIVEDVLDK 456
            RPPPAYELSF VEAKLGHGEVIYVCGSCPSLG+KDPSRALRLVTDPSSYPVWSS PVPVPL +KV+YRYC+VAGGKLKRWEDI+VDRELDPV E  AA  + EDVLDK
Sbjct:    3 RPPPAYELSFGVEAKLGHGEVIYVCGSCPSLGSKDPSRALRLVTDPSSYPVWSSEPVPVPLGQKVTYRYCIVAGGKLKRWEDIAVDRELDPVKEADAAPVMAEDVLDK 110
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BLAST of mRNA_Ecto-sp13_S_contig12756.2284.1 vs. uniprot
Match: A0A835YZM5_9STRA (Glycosyltransferase family 20-domain-containing protein n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835YZM5_9STRA)

HSP 1 Score: 100 bits (249), Expect = 1.030e-21
Identity = 47/94 (50.00%), Postives = 64/94 (68.09%), Query Frame = 1 
Query:  142 PAYELSFAVEAKLGHGEVIYVCGSCPSLGAKDPSRALRLVTDPSSYPVWSSAPVPVPLNKKVSYRYCVVAGGKLKRWEDISVDRELDPVIEPSA 423
            P YE+ F V A+LGHGEV +V G+CPSLG  D   A+ L T P +YP+W S P+ +PL++ V Y+Y V +GG+ KRWE++  DR LD V   +A
Sbjct:    2 PFYEVEFEVTAELGHGEVPFVAGTCPSLGNMDYKSAVLLTTTPDTYPIWRSPPLALPLHQIVLYKYGVFSGGEFKRWEEVIEDRVLDVVTTATA 95
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BLAST of mRNA_Ecto-sp13_S_contig12756.2284.1 vs. uniprot
Match: A0A7S2Y1E9_9STRA (Hypothetical protein (Fragment) n=1 Tax=Fibrocapsa japonica TaxID=94617 RepID=A0A7S2Y1E9_9STRA)

HSP 1 Score: 74.7 bits (182), Expect = 3.490e-13
Identity = 35/82 (42.68%), Postives = 50/82 (60.98%), Query Frame = 1 
Query:  154 LSFAVEAKLGHGEVIYVCGSCPSLGAKDPSRALRLVTDPSSYPVWSSAPVPVPLNKK---VSYRYCVVAGGKLKRWEDISVD 390
            + F V AK+G GE + VCG+C +LG+   S  ++L T P  YP WSS+ VP+ L+     V YRY +  GG+  RWE+ + D
Sbjct:    8 VGFRVNAKVGFGEEVVVCGNCSALGSNVASHGIKLKTSPEEYPYWSSSLVPLALSSNTFDVKYRYGIFRGGEFLRWEESTSD 89
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BLAST of mRNA_Ecto-sp13_S_contig12756.2284.1 vs. uniprot
Match: A0A2R5GMJ6_9STRA (Alpha,alpha-trehalose-phosphate synthase UDP-forming n=1 Tax=Hondaea fermentalgiana TaxID=2315210 RepID=A0A2R5GMJ6_9STRA)

HSP 1 Score: 69.7 bits (169), Expect = 5.740e-11
Identity = 31/88 (35.23%), Postives = 54/88 (61.36%), Query Frame = 1 
Query:  154 LSFAVEAKLGHGEVIYVCGSCPSLGAKDPSRALRLVTDPSSYPVWSSA-PVPVPLNKKVSYRYCVVAGGKLKRWEDISVDRELDPVIE 414
            + F   A +  GE + + G   +LG+ D    ++LVT P  +P W +A P+P+P  + ++YRYC+++GG+L RWED+  D+  +  +E
Sbjct:    9 VGFNAIAPVQRGETVCLVGDVAALGSGDHRYGVQLVTAPDVFPEWYTAQPIPLPAGQVITYRYCILSGGELSRWEDLPGDKPREIFVE 96
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BLAST of mRNA_Ecto-sp13_S_contig12756.2284.1 vs. uniprot
Match: A0A0G4G6Y5_VITBC (CBM20 domain-containing protein n=1 Tax=Vitrella brassicaformis (strain CCMP3155) TaxID=1169540 RepID=A0A0G4G6Y5_VITBC)

HSP 1 Score: 52.0 bits (123), Expect = 6.260e-5
Identity = 27/76 (35.53%), Postives = 42/76 (55.26%), Query Frame = 1 
Query:  151 ELSFAVEAKLGHGEVIYVCGSCPSLGAKDPSRALRLVTDPSSYPVWSSAPVPVPLNKKVSYRYCVVAGGKLKRWED 378
            +++F V      GE + + G+  S+G  DP+  + L TD   YP+W SAPV +P +  + Y+Y V  G    RWE+
Sbjct:    4 KVTFMVHCDTCFGESVKLVGNHSSIGDWDPASGIPLATDDVLYPIWVSAPVELPRDGYIEYKY-VKYGEHHTRWEE 78
The following BLAST results are available for this feature:
BLAST of mRNA_Ecto-sp13_S_contig12756.2284.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 6
Match NameE-valueIdentityDescription
D7FL73_ECTSI3.790e-7097.50Trehalose 6-phosphate synthase, family GT20 / Treh... [more]
V5K3Y9_SACJA5.850e-5586.11Trehalose-6-phosohate synthase n=1 Tax=Saccharina ... [more]
A0A835YZM5_9STRA1.030e-2150.00Glycosyltransferase family 20-domain-containing pr... [more]
A0A7S2Y1E9_9STRA3.490e-1342.68Hypothetical protein (Fragment) n=1 Tax=Fibrocapsa... [more]
A0A2R5GMJ6_9STRA5.740e-1135.23Alpha,alpha-trehalose-phosphate synthase UDP-formi... [more]
A0A0G4G6Y5_VITBC6.260e-535.53CBM20 domain-containing protein n=1 Tax=Vitrella b... [more]
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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
Ecto-sp13_S_contig12756contigEcto-sp13_S_contig12756:1039..1930 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Ectocarpus species13 EcNAP12_S_4_19m OGS1.02022-07-08
Properties
Property NameValue
Taxonomic scopeEukaryota
Stop0
Start0
Seed ortholog score70.5
Seed ortholog evalue7.8e-10
Seed eggNOG ortholog2880.D7G011
Model size458
KEGG rclassRC00005,RC00017,RC00049,RC02748
KEGG koko:K16055
KEGG ReactionR02737,R02778
KEGG Pathwayko00500,ko01100,map00500,map01100
Hectar predicted targeting categoryother localisation
Exons2
EggNOG free text desc.trehalose biosynthetic process
EggNOG OGsCOG1877@1,KOG1050@2759
EC2.4.1.15,3.1.3.12
Cds size456
COG Functional cat.G
CAZyGT20
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko01000,ko01003
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1681462682.3727458-CDS-Ecto-sp13_S_contig12756:1038..11021681462682.3727458-CDS-Ecto-sp13_S_contig12756:1038..1102Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig12756 1039..1102 -
1681462682.3830163-CDS-Ecto-sp13_S_contig12756:1538..19301681462682.3830163-CDS-Ecto-sp13_S_contig12756:1538..1930Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig12756 1539..1930 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_Ecto-sp13_S_contig12756.2284.1prot_Ecto-sp13_S_contig12756.2284.1Ectocarpus species13 EcNAP12_S_4_19mpolypeptideEcto-sp13_S_contig12756 1039..1930 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_Ecto-sp13_S_contig12756.2284.1

>prot_Ecto-sp13_S_contig12756.2284.1 ID=prot_Ecto-sp13_S_contig12756.2284.1|Name=mRNA_Ecto-sp13_S_contig12756.2284.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=polypeptide|length=152bp
TDRLRHLAIDLPVAPPPPKPVGAPQRGPHFGDMANDASSSIMPTRPPPAY
ELSFAVEAKLGHGEVIYVCGSCPSLGAKDPSRALRLVTDPSSYPVWSSAP
VPVPLNKKVSYRYCVVAGGKLKRWEDISVDRELDPVIEPSAATAIVEDVL
DK
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mRNA from alignment at Ecto-sp13_S_contig12756:1039..1930-

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_Ecto-sp13_S_contig12756.2284.1 ID=mRNA_Ecto-sp13_S_contig12756.2284.1|Name=mRNA_Ecto-sp13_S_contig12756.2284.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=mRNA|length=892bp|location=Sequence derived from alignment at Ecto-sp13_S_contig12756:1039..1930- (Ectocarpus species13 EcNAP12_S_4_19m)
ACAGACCGCCTCCGCCACCTCGCTATCGATTTGCCCGTAGCACCACCACC
ACCAAAACCAGTGGGAGCGCCTCAGCGGGGCCCCCATTTTGGAGACATGG
CTAATGACGCCTCGAGTAGCATCATGCCGACCCGCCCGCCTCCCGCGTAC
GAGCTATCCTTCGCCGTGGAGGCCAAGCTGGGCCACGGGGAGGTAATCTA
CGTCTGCGGCTCCTGCCCCTCCCTGGGCGCCAAGGACCCCTCCCGCGCCC
TGCGGCTGGTGACCGACCCGTCGAGCTACCCCGTGTGGAGCTCGGCCCCC
GTCCCGGTGCCCCTTAACAAGAAGGTCTCGTACCGGTACTGCGTGGTTGC
CGGGGGGAAGCTCAAGCGGTGGGAGGACATCTCGGTAGACAGGTGAGGCG
CAGGGGGGGGCGGTAAGCGTACTCTTGTGTTTGGTATGCCTTAGCGGTTA
GACCATTGACCACGCGTCCTCGACAAGGGGGGGGGTTGCAACGGTTGTGT
TGGGCGTGTGGGCTTGCGCTACAGGTTCTTGCGAGTGTTGCATTGTCAGG
GTTAGTTTTCGATGTGCTTTTTTTCAATGTGCTCCTCCGAACGACGTGCC
ATTTTTGTTTCGCTCGTTCGGCCAAAGCATGCACGTGCGCACAGCGGTAT
TGAGATGACCGGCTTGACCTCTTGCCAGTAGCTGTAGGCTCTCAACAAGC
TTCGTTGTCAGCGATGCTGTCCCGCCCTCTGTGTTTTTACAACCCATCCC
ATCTCTGCATGCGTGCCTCGGGCGTCTCGCGGTATTTGATGCCTCTCCCC
TTTTTCACGCCCTCAACACGACACACAGGGAGTTGGATCCGGTGATAGAA
CCATCAGCGGCCACGGCGATCGTCGAGGATGTTCTGGACAAG
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Coding sequence (CDS) from alignment at Ecto-sp13_S_contig12756:1039..1930-

>mRNA_Ecto-sp13_S_contig12756.2284.1 ID=mRNA_Ecto-sp13_S_contig12756.2284.1|Name=mRNA_Ecto-sp13_S_contig12756.2284.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=CDS|length=456bp|location=Sequence derived from alignment at Ecto-sp13_S_contig12756:1039..1930- (Ectocarpus species13 EcNAP12_S_4_19m)
ACAGACCGCCTCCGCCACCTCGCTATCGATTTGCCCGTAGCACCACCACC
ACCAAAACCAGTGGGAGCGCCTCAGCGGGGCCCCCATTTTGGAGACATGG
CTAATGACGCCTCGAGTAGCATCATGCCGACCCGCCCGCCTCCCGCGTAC
GAGCTATCCTTCGCCGTGGAGGCCAAGCTGGGCCACGGGGAGGTAATCTA
CGTCTGCGGCTCCTGCCCCTCCCTGGGCGCCAAGGACCCCTCCCGCGCCC
TGCGGCTGGTGACCGACCCGTCGAGCTACCCCGTGTGGAGCTCGGCCCCC
GTCCCGGTGCCCCTTAACAAGAAGGTCTCGTACCGGTACTGCGTGGTTGC
CGGGGGGAAGCTCAAGCGGTGGGAGGACATCTCGGTAGACAGGGAGTTGG
ATCCGGTGATAGAACCATCAGCGGCCACGGCGATCGTCGAGGATGTTCTG
GACAAG
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