mRNA_Ecto-sp13_S_contig12746.2275.1 (mRNA) Ectocarpus species13 EcNAP12_S_4_19m

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_Ecto-sp13_S_contig12746.2275.1
Unique NamemRNA_Ecto-sp13_S_contig12746.2275.1
TypemRNA
OrganismEctocarpus species13 EcNAP12_S_4_19m (Ectocarpus species13 EcNAP12_S_4_19m)
Homology
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BLAST of mRNA_Ecto-sp13_S_contig12746.2275.1 vs. uniprot
Match: A0A836CMU5_9STRA (Ubiquitin carboxyl-terminal hydrolase n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A836CMU5_9STRA)

HSP 1 Score: 160 bits (405), Expect = 1.480e-43
Identity = 76/104 (73.08%), Postives = 87/104 (83.65%), Query Frame = 1 
Query:    1 LPTIVTQAVDAIIEHQGAAEQTEVSQWVADNELIVSKYADTLEHVDSGRRISSDPKTWVCEDSGKTENLWLNLSTGYIGSGRQNWDGSGGTGAALRHYEETGRK 312
            LPT+V QA DA+I HQGAAEQ EV+ W A+ EL VSKYA+ L   D+G RIS+DP +W C DSG TENLWLNLSTGYIGSGR+NWDGSGG+GAAL+HYEETGRK
Sbjct:  161 LPTLVCQAADAVITHQGAAEQAEVASWEAEQELPVSKYAENLPVEDNGVRISNDPSSWKCADSGVTENLWLNLSTGYIGSGRRNWDGSGGSGAALKHYEETGRK 264
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BLAST of mRNA_Ecto-sp13_S_contig12746.2275.1 vs. uniprot
Match: A0A7S2V2B3_9STRA (Ubiquitinyl hydrolase 1 (Fragment) n=1 Tax=Fibrocapsa japonica TaxID=94617 RepID=A0A7S2V2B3_9STRA)

HSP 1 Score: 142 bits (359), Expect = 3.660e-40
Identity = 65/103 (63.11%), Postives = 81/103 (78.64%), Query Frame = 1 
Query:    1 LPTIVTQAVDAIIEHQGAAEQTEVSQWVADNELIVSKYADTLEHVDSGRRISSDPKTWVCEDSGKTENLWLNLSTGYIGSGRQNWDGSGGTGAALRHYEETGR 309
            LP +V QAV+AII HQGAA   +V QW  D ++ VSKYA+ L  +D+G++IS DP TW CE+SG  ENLWLNLSTG+IGSGR  WDG+GGTGAAL+H+ ETG+
Sbjct:   37 LPELVLQAVEAIIAHQGAATMDQVDQWTMDEDVPVSKYAENLVQLDNGKKISPDPATWACEESGMKENLWLNLSTGHIGSGRPMWDGTGGTGAALKHFNETGQ 139
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BLAST of mRNA_Ecto-sp13_S_contig12746.2275.1 vs. uniprot
Match: A0A7R9Y9N6_9STRA (Ubiquitin carboxyl-terminal hydrolase n=1 Tax=Pinguiococcus pyrenoidosus TaxID=172671 RepID=A0A7R9Y9N6_9STRA)

HSP 1 Score: 145 bits (366), Expect = 2.680e-38
Identity = 66/104 (63.46%), Postives = 82/104 (78.85%), Query Frame = 1 
Query:    1 LPTIVTQAVDAIIEHQGAAEQTEVSQWVADNELIVSKYADTLEHVDSGRRISSDPKTWVCEDSGKTENLWLNLSTGYIGSGRQNWDGSGGTGAALRHYEETGRK 312
            +P +V      +IEHQG ++Q +++QWV D EL  SKYA+ LE +D+G +ISSDP TW CEDSG TENLWLNLSTGYIGSGR+NWDGSGGT  AL+H+E TG+K
Sbjct:  145 IPEMVVVVARQVIEHQGYSDQQDLAQWVDDEELKESKYAEALEQLDNGVKISSDPSTWKCEDSGMTENLWLNLSTGYIGSGRRNWDGSGGTNGALKHFEATGQK 248
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BLAST of mRNA_Ecto-sp13_S_contig12746.2275.1 vs. uniprot
Match: D8LDI4_ECTSI (Ubiquitin carboxyl-terminal hydrolase n=2 Tax=Ectocarpus TaxID=2879 RepID=D8LDI4_ECTSI)

HSP 1 Score: 145 bits (366), Expect = 2.860e-38
Identity = 83/135 (61.48%), Postives = 88/135 (65.19%), Query Frame = 1 
Query:    1 LPTIVTQAVDAIIEHQGAAEQTEVSQWVADNELIVSKYADTLEHVDSGRRISSDPKTWVCEDSGKTEN-------------------------------LWLNLSTGYIGSGRQNWDGSGGTGAALRHYEETGRK 312
            LPTIV+QAVDAIIEHQGAAEQTE SQWVADNELIVSKYADTLEHVDSGRRISS+P+        K                                  L+  +  GYIGSGRQNWDGSGGTGAALRHYEETGRK
Sbjct:  162 LPTIVSQAVDAIIEHQGAAEQTEGSQWVADNELIVSKYADTLEHVDSGRRISSEPQDVGVRKQRKDGEPLAQPVHRQAPCGVSSRDRCLEVTKPVPYVLLFPRVFPGYIGSGRQNWDGSGGTGAALRHYEETGRK 296
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BLAST of mRNA_Ecto-sp13_S_contig12746.2275.1 vs. uniprot
Match: A0A7R9VT46_9CHLO (Ubiquitinyl hydrolase 1 (Fragment) n=1 Tax=Chlamydomonas euryale TaxID=1486919 RepID=A0A7R9VT46_9CHLO)

HSP 1 Score: 138 bits (347), Expect = 6.640e-37
Identity = 65/104 (62.50%), Postives = 82/104 (78.85%), Query Frame = 1 
Query:    1 LPTIVTQAVDAIIEHQGAAEQTEVSQWVADNELIVSKYADTLEHVDSGRRISSDPKTWVCEDSGKTENLWLNLSTGYIGSGRQNWDGSGGTGAALRHYEETGRK 312
            LP +V Q VDAI +H  A++Q EV+ W  + E  VS+YA+ L  +D+GRRIS DPK W CE++G+T NLWLNLSTG+IGSGR+NWDGSGG G+ALRH+E TGRK
Sbjct:  138 LPELVLQVVDAITKHDAASDQAEVAVW--EEEAKVSRYAEGLPQLDTGRRISPDPKDWRCEETGETGNLWLNLSTGFIGSGRRNWDGSGGNGSALRHFEATGRK 239
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BLAST of mRNA_Ecto-sp13_S_contig12746.2275.1 vs. uniprot
Match: A0A2R5GGB5_9STRA (Ubiquitinyl hydrolase 1 n=1 Tax=Hondaea fermentalgiana TaxID=2315210 RepID=A0A2R5GGB5_9STRA)

HSP 1 Score: 138 bits (348), Expect = 7.600e-36
Identity = 69/105 (65.71%), Postives = 80/105 (76.19%), Query Frame = 1 
Query:    1 LPTIVTQAVDAIIEHQGAAEQTEVSQWVADNELI-VSKYADTLEHVDSGRRISSDPKTWVCEDSGKTENLWLNLSTGYIGSGRQNWDGSGGTGAALRHYEETGRK 312
            LP  + +A DAII  + A+ Q E   W A  E   VSK+A+ LE +D+G RIS DPKTW CEDSG TENLWLNLSTGYIGSGR+NWDGSGGTGAAL+H+E TGRK
Sbjct:  151 LPASLLEAADAIIAMEDASSQREFEAWDASEEKRPVSKFAENLEQLDNGVRISPDPKTWRCEDSGLTENLWLNLSTGYIGSGRRNWDGSGGTGAALKHFEATGRK 255
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BLAST of mRNA_Ecto-sp13_S_contig12746.2275.1 vs. uniprot
Match: A0A7S1SZS2_9CHLO (Ubiquitin carboxyl-terminal hydrolase n=1 Tax=Tetraselmis chuii TaxID=63592 RepID=A0A7S1SZS2_9CHLO)

HSP 1 Score: 133 bits (335), Expect = 4.310e-34
Identity = 62/105 (59.05%), Postives = 83/105 (79.05%), Query Frame = 1 
Query:    1 LPTIVTQAVDAIIEHQGAAEQTEVSQWVADNELIVSKYADTLEHVDS-GRRISSDPKTWVCEDSGKTENLWLNLSTGYIGSGRQNWDGSGGTGAALRHYEETGRK 312
            LP +V QA++A++ H+ A +Q EVS W  + E  VSKYA++LE + + G+ I SDP  W C++SG TENLWLNLSTG+IGSGRQNWDGSGG GAA+RH+++TG+K
Sbjct:  135 LPEVVLQAINAVVAHEAAGKQEEVSVW--EEERAVSKYAESLEQLPANGKVIPSDPSQWKCDESGATENLWLNLSTGFIGSGRQNWDGSGGNGAAIRHFDQTGKK 237
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BLAST of mRNA_Ecto-sp13_S_contig12746.2275.1 vs. uniprot
Match: A0A7R9TGP0_9VIRI (Ubiquitin carboxyl-terminal hydrolase n=2 Tax=Prasinoderma coloniale TaxID=156133 RepID=A0A7R9TGP0_9VIRI)

HSP 1 Score: 132 bits (332), Expect = 1.090e-33
Identity = 64/104 (61.54%), Postives = 77/104 (74.04%), Query Frame = 1 
Query:    1 LPTIVTQAVDAIIEHQGAAEQTEVSQWVADNELIVSKYADTLEHVDSGRRISSDPKTWVCEDSGKTENLWLNLSTGYIGSGRQNWDGSGGTGAALRHYEETGRK 312
            LP  V +A + ++   GA     V  +VAD E  VSKYA++LE +D+G +IS DP TW CE+SG TENLWLNLSTG+IGSGR+NWDGSGGTGAAL HYE TG K
Sbjct:  151 LPERVIEAANGVLAAAGAERTAAVDAFVADEERKVSKYAESLEQLDNGVKISPDPTTWKCEESGMTENLWLNLSTGHIGSGRRNWDGSGGTGAALTHYEATGSK 254
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BLAST of mRNA_Ecto-sp13_S_contig12746.2275.1 vs. uniprot
Match: A0A448ZMX3_9STRA (UBP-type domain-containing protein n=1 Tax=Pseudo-nitzschia multistriata TaxID=183589 RepID=A0A448ZMX3_9STRA)

HSP 1 Score: 125 bits (315), Expect = 1.950e-33
Identity = 57/104 (54.81%), Postives = 76/104 (73.08%), Query Frame = 1 
Query:    1 LPTIVTQAVDAIIEHQGAAEQTEVSQWVADNELIVSKYADTLEHVDSGRRISSDPKTWVCEDSGKTENLWLNLSTGYIGSGRQNWDGSGGTGAALRHYEETGRK 312
            LP +V ++  +II H G A Q +++ W  D E+ +SKY + L  VD+G +IS DPK+W CE SG TEN+WLNLS G+IG GR+NWDGSGG+  AL HYEETG++
Sbjct:  103 LPEMVVKSAKSIIHHVGLAIQQDLTAWKDDEEIPISKYCNELPFVDNGVKISPDPKSWKCEKSGDTENIWLNLSDGFIGGGRKNWDGSGGSNGALDHYEETGQQ 206
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BLAST of mRNA_Ecto-sp13_S_contig12746.2275.1 vs. uniprot
Match: A0A2P6U3W2_CHLSO (Ubiquitin carboxyl-terminal hydrolase n=1 Tax=Chlorella sorokiniana TaxID=3076 RepID=A0A2P6U3W2_CHLSO)

HSP 1 Score: 131 bits (329), Expect = 2.790e-33
Identity = 64/104 (61.54%), Postives = 76/104 (73.08%), Query Frame = 1 
Query:    1 LPTIVTQAVDAIIEHQGAAEQTEVSQWVADNELIVSKYADTLEHVDSGRRISSDPKTWVCEDSGKTENLWLNLSTGYIGSGRQNWDGSGGTGAALRHYEETGRK 312
            LP +V   + A+ EH  AA+Q +V  W  + E  VSKYAD LE + + +RI  DPK W CEDSG  ENLWLNLSTGYIGSGR NWDG+GGTGAAL+HYE TG+K
Sbjct:  134 LPELVLGCIKAVQEHASAAQQEKVDAW--EEERRVSKYADGLEQLPATKRIPMDPKQWRCEDSGLQENLWLNLSTGYIGSGRANWDGTGGTGAALKHYEATGKK 235
The following BLAST results are available for this feature:
BLAST of mRNA_Ecto-sp13_S_contig12746.2275.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A836CMU5_9STRA1.480e-4373.08Ubiquitin carboxyl-terminal hydrolase n=1 Tax=Trib... [more]
A0A7S2V2B3_9STRA3.660e-4063.11Ubiquitinyl hydrolase 1 (Fragment) n=1 Tax=Fibroca... [more]
A0A7R9Y9N6_9STRA2.680e-3863.46Ubiquitin carboxyl-terminal hydrolase n=1 Tax=Ping... [more]
D8LDI4_ECTSI2.860e-3861.48Ubiquitin carboxyl-terminal hydrolase n=2 Tax=Ecto... [more]
A0A7R9VT46_9CHLO6.640e-3762.50Ubiquitinyl hydrolase 1 (Fragment) n=1 Tax=Chlamyd... [more]
A0A2R5GGB5_9STRA7.600e-3665.71Ubiquitinyl hydrolase 1 n=1 Tax=Hondaea fermentalg... [more]
A0A7S1SZS2_9CHLO4.310e-3459.05Ubiquitin carboxyl-terminal hydrolase n=1 Tax=Tetr... [more]
A0A7R9TGP0_9VIRI1.090e-3361.54Ubiquitin carboxyl-terminal hydrolase n=2 Tax=Pras... [more]
A0A448ZMX3_9STRA1.950e-3354.81UBP-type domain-containing protein n=1 Tax=Pseudo-... [more]
A0A2P6U3W2_CHLSO2.790e-3361.54Ubiquitin carboxyl-terminal hydrolase n=1 Tax=Chlo... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
Ecto-sp13_S_contig12746contigEcto-sp13_S_contig12746:629..2417 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Ectocarpus species13 EcNAP12_S_4_19m OGS1.02022-07-08
Properties
Property NameValue
Taxonomic scopeEukaryota
Stop1
Start0
Seed ortholog score147.1
Seed ortholog evalue4.5e-33
Seed eggNOG ortholog2880.D8LDI4
Model size315
KEGG koko:K11836
Hectar predicted targeting categoryother localisation
GOsGO:0000323,GO:0003674,GO:0003824,GO:0004175,GO:0004197,GO:0004843,GO:0005488,GO:0005515,GO:0005575,GO:0005622,GO:0005623,GO:0005737,GO:0005764,GO:0005773,GO:0005829,GO:0006464,GO:0006508,GO:0006807,GO:0008150,GO:0008152,GO:0008233,GO:0008234,GO:0009893,GO:0009894,GO:0009896,GO:0009987,GO:0010604,GO:0016579,GO:0016787,GO:0019222,GO:0019538,GO:0019783,GO:0030162,GO:0031323,GO:0031325,GO:0031329,GO:0031331,GO:0032182,GO:0032268,GO:0032270,GO:0032434,GO:0032436,GO:0036211,GO:0036459,GO:0042176,GO:0043130,GO:0043170,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0043412,GO:0044237,GO:0044238,GO:0044260,GO:0044267,GO:0044424,GO:0044444,GO:0044464,GO:0045732,GO:0045862,GO:0048518,GO:0048522,GO:0050789,GO:0050794,GO:0051171,GO:0051173,GO:0051246,GO:0051247,GO:0060255,GO:0061136,GO:0065007,GO:0070011,GO:0070646,GO:0070647,GO:0071108,GO:0071704,GO:0080090,GO:0101005,GO:0140096,GO:1901564,GO:1901800,GO:1903050,GO:1903052,GO:1903362,GO:1903364,GO:2000058,GO:2000060
Exons3
EggNOG free text desc.Pfam Zn-finger in ubiquitin-hydrolases and other protein
EggNOG OGsCOG5207@1,KOG0944@2759
EC3.4.19.12
Cds size315
COG Functional cat.O
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko01000,ko01002,ko04147
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1681462681.8852477-CDS-Ecto-sp13_S_contig12746:628..6971681462681.8852477-CDS-Ecto-sp13_S_contig12746:628..697Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig12746 629..697 +
1681462681.8949294-CDS-Ecto-sp13_S_contig12746:1594..17511681462681.8949294-CDS-Ecto-sp13_S_contig12746:1594..1751Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig12746 1595..1751 +
1681462681.903104-CDS-Ecto-sp13_S_contig12746:2328..24171681462681.903104-CDS-Ecto-sp13_S_contig12746:2328..2417Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig12746 2329..2417 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_Ecto-sp13_S_contig12746.2275.1prot_Ecto-sp13_S_contig12746.2275.1Ectocarpus species13 EcNAP12_S_4_19mpolypeptideEcto-sp13_S_contig12746 629..2417 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_Ecto-sp13_S_contig12746.2275.1

>prot_Ecto-sp13_S_contig12746.2275.1 ID=prot_Ecto-sp13_S_contig12746.2275.1|Name=mRNA_Ecto-sp13_S_contig12746.2275.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=polypeptide|length=105bp
LPTIVTQAVDAIIEHQGAAEQTEVSQWVADNELIVSKYADTLEHVDSGRR
ISSDPKTWVCEDSGKTENLWLNLSTGYIGSGRQNWDGSGGTGAALRHYEE
TGRK*
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mRNA from alignment at Ecto-sp13_S_contig12746:629..2417+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_Ecto-sp13_S_contig12746.2275.1 ID=mRNA_Ecto-sp13_S_contig12746.2275.1|Name=mRNA_Ecto-sp13_S_contig12746.2275.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=mRNA|length=1789bp|location=Sequence derived from alignment at Ecto-sp13_S_contig12746:629..2417+ (Ectocarpus species13 EcNAP12_S_4_19m)
CTGCCAACGATCGTTACCCAGGCGGTCGACGCGATCATCGAGCATCAAGG
CGCGGCAGAACAGACTGAGGTATGGCAATGGTATGCATGAGCCTCAAAGT
CGATTGATTGTTATCCTGTTGTCTCTTATGTTTGATTTTTGCGAGCGGGA
TGGTCGTTGGAGGCCTTCGGCTGTGCGCATGCGCACTTAGAAGAGCAGAA
ATACGGAGGGATGGCTAGGAAGAGCTGAACAGAGGTGTAGGTTACGTACG
CCACGAATAGCTTGTACCAGTTGCAGCAGTGCTGTTGAAGGGTGTGGCTC
CGTGGCGTCTTCACCTTGAAGCAAGGTCGACGGCCAAGCTTGTCCTTAGG
CACGTCTCGAGAGAAGGAGGCGGGCGCTGGTGGATCAGCAGTCGCACTGT
TGTACCGAAGCGTGCGTGCGTGCCTTGGACGTGATGCGATCTCTCGACGA
AGGTGCTTCTCGCTGTGAGAGTTAATCGCCAACTGGCACAGAGCTGGGGC
CCTCTCGAGAATGGGGGTGGTAGGTTGATCTAGAACCCTCTCGAAAAAGG
GGGGGCAGGCTTTGAGAATCTGATCGCTTGTTCGGGCTGCCTCGCCCTCG
TGTTTGCGATAATCCTAGCGGTTCGTTGGCCCGAGCGAACGCGCCCGCCT
ATAGCGACGGCAGCGCACAAGCCAGAGATTTCTGGGCTGGTCAGACGACT
ACGTGGGCGATCTACAGCACTATTTGCGATAAAGGTCTATGTGTTGCGCC
AAAGCAACGAGAAAGATCAGCTAGAGGCACCGGTTGTGCTGCTATCGCTC
CTCCTGTGTACATAGGTCCACGGTCGGACGGTTTCTTCTCGATGAAATTC
CGTAACAGGTGTCTCTTGATTCGGCGTTGCCCCCCTTGGTAGGCGCATAT
TGATTCGGCGTTGCCCCCTTTGGTAGGTGCCTAATGATTCGGCGTTGCTC
CCCCCTTTCATTCCAGGTGAGCCAGTGGGTGGCCGACAACGAGCTTATCG
TGAGCAAGTACGCCGACACATTGGAGCACGTCGACAGCGGCCGCAGGATC
TCTTCCGACCCCAAGACGTGGGTGTGCGAAGACAGCGGAAAGACCGAGAA
CCTCTGGCTCAACCTGTCCACTGGTAAGCGAAGAATAGAGCCAGCCAGCT
TGCTTTGAACGTTAACGTTACGCTGAGCCTTGATCGCTACAGCAGTACCC
TAGCTCGCCCAAGGTTCGGTGACGAAGAATTAACAACCTTCAGGTTTACT
TCTGGGCACCCGTTCAGATTATGGAGCATGCCTTGACGGAGCTCTGTTGG
CCTCTAGTTCACGTGATGGTAAAATGTAGGTGACTTTGCAATGCCCTTTC
CCCTTGGGGCAGATGGCAGTTGTATGTCGAGGCCAATGTTCCCCAATCGC
TAAGATCGTCGTCAATCACCAAGCTGTTGGCGCAAGACCTGTTTTCTGTT
CCCTTCTCTATGCAGAGATAGAGTGCAGGACACGTTTGCTTAGCGAGAGT
CCATGATGGTGTATGACGTACATCTCTTTCAAAAAAATCCAAGTTGAAGT
GTATGGACCAAACGCCGTGTTACCTGCATTGAACGCTACTTTGGGGCTGT
TTGTGAAAGTCCAGCACCGTGCGGGATTTCCAGGCAGGATTACTGTCTCG
AGGTCACCAAGCCAGTTCCGTATGTTTTTCATTTTCCGTGGGTTTTCCAG
GCTACATCGGGTCGGGGCGTCAGAACTGGGACGGTTCTGGAGGAACCGGA
GCAGCGCTCCGGCACTATGAGGAGACCGGGAGAAAGTGA
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Coding sequence (CDS) from alignment at Ecto-sp13_S_contig12746:629..2417+

>mRNA_Ecto-sp13_S_contig12746.2275.1 ID=mRNA_Ecto-sp13_S_contig12746.2275.1|Name=mRNA_Ecto-sp13_S_contig12746.2275.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=CDS|length=315bp|location=Sequence derived from alignment at Ecto-sp13_S_contig12746:629..2417+ (Ectocarpus species13 EcNAP12_S_4_19m)
CTGCCAACGATCGTTACCCAGGCGGTCGACGCGATCATCGAGCATCAAGG
CGCGGCAGAACAGACTGAGGTGAGCCAGTGGGTGGCCGACAACGAGCTTA
TCGTGAGCAAGTACGCCGACACATTGGAGCACGTCGACAGCGGCCGCAGG
ATCTCTTCCGACCCCAAGACGTGGGTGTGCGAAGACAGCGGAAAGACCGA
GAACCTCTGGCTCAACCTGTCCACTGGCTACATCGGGTCGGGGCGTCAGA
ACTGGGACGGTTCTGGAGGAACCGGAGCAGCGCTCCGGCACTATGAGGAG
ACCGGGAGAAAGTGA
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