mRNA_Ecto-sp13_S_contig9827.21474.1 (mRNA) Ectocarpus species13 EcNAP12_S_4_19m

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_Ecto-sp13_S_contig9827.21474.1
Unique NamemRNA_Ecto-sp13_S_contig9827.21474.1
TypemRNA
OrganismEctocarpus species13 EcNAP12_S_4_19m (Ectocarpus species13 EcNAP12_S_4_19m)
Homology
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BLAST of mRNA_Ecto-sp13_S_contig9827.21474.1 vs. uniprot
Match: A0A6H5JSI9_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JSI9_9PHAE)

HSP 1 Score: 404 bits (1037), Expect = 1.980e-131
Identity = 205/223 (91.93%), Postives = 210/223 (94.17%), Query Frame = 2 
Query:    2 THWTWYATTSYFVVSALSAFLCWRAITVTVASGPVRNSDSAFAAPTSSRPPDRTGSPQPVPAALRFLQLALWSTACISSLIVVAIFWGALYDGDKLTTVDEQAHAFVACIMLLDQFLVADEFKLRYILFPQAFGVIYILFNVGWYYLAPDDDRLIYIILDWEDNTLVACIYAFGAILVGAPLFGLFHLGIFRLREKVYQNRLARSSKAAAEAQAIELKESDQA 670
            THWTWYATT+YFVVSALSAFLCWRAI VTVASGPVR+ D AF APTSSRP DRTGSPQPVPAALR LQLALWSTACI SLIVV+IFWGALYDGDKLTTVDEQAH FVACIMLLDQFLVADEFKLRY+LFPQAFGVIYI FNVGWYYLAPDDDRLIYIILDWEDNTL ACIYAFGAILVGAPLFGL H GIFRLRE+VY NRLARSSKAAAEAQAIELKESDQA
Sbjct:   60 THWTWYATTTYFVVSALSAFLCWRAIAVTVASGPVRDGDPAFDAPTSSRPSDRTGSPQPVPAALRHLQLALWSTACIVSLIVVSIFWGALYDGDKLTTVDEQAHVFVACIMLLDQFLVADEFKLRYVLFPQAFGVIYIFFNVGWYYLAPDDDRLIYIILDWEDNTLGACIYAFGAILVGAPLFGLVHFGIFRLRERVYHNRLARSSKAAAEAQAIELKESDQA 282
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BLAST of mRNA_Ecto-sp13_S_contig9827.21474.1 vs. uniprot
Match: D7G734_ECTSI (Uncharacterized protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7G734_ECTSI)

HSP 1 Score: 265 bits (677), Expect = 2.970e-80
Identity = 140/176 (79.55%), Postives = 144/176 (81.82%), Query Frame = 2 
Query:  143 SRPPDRTGSPQPVPAALRFLQLALWSTACISSLIVVAIFWGALYDGDKLTTVDEQAHAFVACIMLLDQFLVADEFKLRYILFPQAFGVIYILFNVGWYYLAPDDDRLIYIILDWEDNTLVACIYAFGAILVGAPLFGLFHLGIFRLREKVYQNRLARSSKAAAEAQAIELKESDQA 670
            SRP DRTGSPQPVPA LR LQL LWSTACI SLIVV IFWGAL                     LLDQFLVADEFKLRYILFPQAFGVIY+ FNVGWYYLAPDDDRLIYIILDWEDNTL ACIYAFGAILVGAPLFGL HLG+FRLRE VY NRLAR+SKAAAEAQ+IELKESDQA
Sbjct:    3 SRPYDRTGSPQPVPAVLRHLQLVLWSTACIGSLIVVTIFWGAL---------------------LLDQFLVADEFKLRYILFPQAFGVIYLFFNVGWYYLAPDDDRLIYIILDWEDNTLGACIYAFGAILVGAPLFGLVHLGLFRLRESVYHNRLARTSKAAAEAQSIELKESDQA 157
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BLAST of mRNA_Ecto-sp13_S_contig9827.21474.1 vs. uniprot
Match: A0A6H5JMZ5_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JMZ5_9PHAE)

HSP 1 Score: 122 bits (306), Expect = 3.810e-27
Identity = 70/202 (34.65%), Postives = 112/202 (55.45%), Query Frame = 2 
Query:    2 THWTWYATTSYFVVSALSAFLCWRAITVTVASGPVRNSDSAFAAPTSSRPPDRTGSPQPVPAALRFL---QLALWSTACISSLIVVAIFWGALYDGDKLTTVDEQAHAFVACIMLLDQFLVADEFKLRYILFPQAFGVIYILFNVGWYYLAPDDDRLIYIILDWEDNTLVACIYAFGAILVGAPLFGLFHLGIFRLREKVYQ 598
            T+W WY    YF+VS  +A+    +     + GPVR + +          P R    QP+ +  R L   Q+  W+ AC+ SL++V +FW AL+D   LT+ D  AH  +A +M +DQ LVA +F+ + +L    F ++Y+ FN+ ++  AP+D+R+IY  +DW ++   +  +A     V  PL GL H  +FRLRE +Y+
Sbjct:   64 TYWGWYMAAIYFLVSTATAYSFLGS--PMASDGPVRGTHAVA--------PHRGNRNQPLSSNRRALLGFQVFSWNVACVGSLVIVTVFWVALFDNTPLTSTDVNAHILIAPVMAIDQLLVATKFERKQMLASFLFSIVYVAFNIVFFLTAPEDERVIYGQMDWGESVGNSAAFAAVVTFVLVPLGGLIHYCVFRLREYIYE 255
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BLAST of mRNA_Ecto-sp13_S_contig9827.21474.1 vs. uniprot
Match: D7G733_ECTSI (Uncharacterized protein n=2 Tax=Ectocarpus TaxID=2879 RepID=D7G733_ECTSI)

HSP 1 Score: 123 bits (308), Expect = 1.210e-26
Identity = 68/196 (34.69%), Postives = 106/196 (54.08%), Query Frame = 2 
Query:    8 WTWYATTSYFVVSALSAFLCWRAITVTVASGPVRNSDSAFAAPTSSRPPDRTGSPQPVPAALRFLQLALWSTACISSLIVVAIFWGALYDGDKLTTVDEQAHAFVACIMLLDQFLVADEFKLRYILFPQAFGVIYILFNVGWYYLAPDDDRLIYIILDWEDNTLVACIYAFGAILVGAPLFGLFHLGIFRLREKVY 595
            WT    T+YFV+S +S+ LC   ++        R   +        RPP   G+  PVP   R+LQ   W   CISS++V+++FW  +Y+ D   T    +   +A +MLLDQFL+A E K +Y      F V+YI +NV +YY    +DR+ + + DW++    AC++ F  +  G P F   H+ ++RLRE++Y
Sbjct:   80 WTHMLATAYFVISLISSLLCMAYLS--------REKPAPVEEGKPKRPPPVKGTRSPVPCYFRWLQHISWVLTCISSMVVLSVFWMTIYESDVPVTSVYVSSTILALLMLLDQFLIATEMKFKYAWLGLYFFVVYIFYNVIYYYEHNVEDRVTFEVFDWDERPGRACLWVFLILAFGVPGFTALHVFVYRLRERMY 267
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BLAST of mRNA_Ecto-sp13_S_contig9827.21474.1 vs. uniprot
Match: D7G735_ECTSI (Uncharacterized protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7G735_ECTSI)

HSP 1 Score: 113 bits (282), Expect = 6.400e-25
Identity = 62/170 (36.47%), Postives = 98/170 (57.65%), Query Frame = 2 
Query:   98 GPVRNSDSAFAAPTSSRPPDRTGSPQPVPAALRFL---QLALWSTACISSLIVVAIFWGALYDGDKLTTVDEQAHAFVACIMLLDQFLVADEFKLRYILFPQAFGVIYILFNVGWYYLAPDDDRLIYIILDWEDNTLVACIYAFGAILVGAPLFGLFHLGIFRLREKVYQ 598
            GPVR + +          P R    QP+ +  R L   Q+  W+ AC+ SL++V +FW AL+D   LT+ D  AH  +A IM +DQ LVA +F+ + +L   +F ++Y+ FN+ ++  AP+D+R+IY  +DW ++   +  +A     V  PL GL H  +FRLRE +Y+
Sbjct:    5 GPVRGTHAVT--------PHRGNRNQPLSSNRRALLGFQVFSWNVACVGSLVIVTVFWVALFDNTPLTSTDVNAHILIAPIMAIDQLLVATKFERKQMLASFSFSIVYVAFNIIFFLTAPEDERVIYGQMDWGESVGNSAAFAAVVAFVLVPLGGLIHYCVFRLREYIYE 166
The following BLAST results are available for this feature:
BLAST of mRNA_Ecto-sp13_S_contig9827.21474.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 5
Match NameE-valueIdentityDescription
A0A6H5JSI9_9PHAE1.980e-13191.93Uncharacterized protein n=1 Tax=Ectocarpus sp. CCA... [more]
D7G734_ECTSI2.970e-8079.55Uncharacterized protein n=1 Tax=Ectocarpus silicul... [more]
A0A6H5JMZ5_9PHAE3.810e-2734.65Uncharacterized protein n=1 Tax=Ectocarpus sp. CCA... [more]
D7G733_ECTSI1.210e-2634.69Uncharacterized protein n=2 Tax=Ectocarpus TaxID=2... [more]
D7G735_ECTSI6.400e-2536.47Uncharacterized protein n=1 Tax=Ectocarpus silicul... [more]
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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
Ecto-sp13_S_contig9827contigEcto-sp13_S_contig9827:1397..5638 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Ectocarpus species13 EcNAP12_S_4_19m OGS1.02022-07-08
Properties
Property NameValue
Stop1
Start1
Model size2637
Hectar predicted targeting categoryother localisation
Exons4
Cds size342
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_Ecto-sp13_S_contig9827.21474.1prot_Ecto-sp13_S_contig9827.21474.1Ectocarpus species13 EcNAP12_S_4_19mpolypeptideEcto-sp13_S_contig9827 3361..4663 -


The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1681464245.9714298-UTR-Ecto-sp13_S_contig9827:1396..33601681464245.9714298-UTR-Ecto-sp13_S_contig9827:1396..3360Ectocarpus species13 EcNAP12_S_4_19mUTREcto-sp13_S_contig9827 1397..3360 -
1681464246.0195267-UTR-Ecto-sp13_S_contig9827:4663..47231681464246.0195267-UTR-Ecto-sp13_S_contig9827:4663..4723Ectocarpus species13 EcNAP12_S_4_19mUTREcto-sp13_S_contig9827 4664..4723 -
1681464246.0307639-UTR-Ecto-sp13_S_contig9827:5367..56381681464246.0307639-UTR-Ecto-sp13_S_contig9827:5367..5638Ectocarpus species13 EcNAP12_S_4_19mUTREcto-sp13_S_contig9827 5368..5638 -


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1681464245.9837136-CDS-Ecto-sp13_S_contig9827:3360..34571681464245.9837136-CDS-Ecto-sp13_S_contig9827:3360..3457Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig9827 3361..3457 -
1681464245.9957235-CDS-Ecto-sp13_S_contig9827:4133..43751681464245.9957235-CDS-Ecto-sp13_S_contig9827:4133..4375Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig9827 4134..4375 -
1681464246.0072937-CDS-Ecto-sp13_S_contig9827:4660..46631681464246.0072937-CDS-Ecto-sp13_S_contig9827:4660..4663Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig9827 4661..4663 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_Ecto-sp13_S_contig9827.21474.1

>prot_Ecto-sp13_S_contig9827.21474.1 ID=prot_Ecto-sp13_S_contig9827.21474.1|Name=mRNA_Ecto-sp13_S_contig9827.21474.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=polypeptide|length=114bp
MLLDQFLVADEFKLRYILFPQAFGVIYILFNVGWYYLAPDDDRLIYIILD
WEDNTLVACIYAFGAILVGAPLFGLFHLGIFRLREKVYQNRLARSSKAAA
EAQAIELKESDQA*
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mRNA from alignment at Ecto-sp13_S_contig9827:1397..5638-

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_Ecto-sp13_S_contig9827.21474.1 ID=mRNA_Ecto-sp13_S_contig9827.21474.1|Name=mRNA_Ecto-sp13_S_contig9827.21474.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=mRNA|length=4242bp|location=Sequence derived from alignment at Ecto-sp13_S_contig9827:1397..5638- (Ectocarpus species13 EcNAP12_S_4_19m)
GACACACTGGACCTGGTACGCTACGACCAGCTACTTTGTCGTTTCTGCCT
TGTCGGCCTTCCTGTGCTGGAGAGCGATTACGGTTACGGTGGCCAGCGGG
CCCGTCAGGAATAGCGACTCAGCGTTCGCCGCACCCACATCATCAAGGCC
GCCCGACCGGACTGGCTCCCCGCAGCCCGTGCCGGCGGCGCTAAGGTTTC
TGCAGCTCGCGCTCTGGAGCACGGCGTGCATAAGCTCACTAATCGTCGTG
GCCATATTTTGGGGGGCCTTGGTGAGTAGTCTGCAGGTCCAGCTCGGAGT
GTTTTTTTGTTCAGCTTTGTGATTTTGTGGTCGTTGTTTTTTCCTTCCGT
CATTCTTGGTGGATGGGCCTCTGCGTAGCATGCCTGAGACGATGTGTAGC
CGGTAAACTCGTCACGTGCGATGCGCGCTCTCGTAGAAAGGGTGAAAAGG
AAGGGAGGAGGACCTTCGCAAATGGGTCACTCAGAATTCGCGGCGCACTG
GCCGTGTGTTTGCTCCACAGGAACAAATATCAAAATGTCAAAATATGCCT
GCTTCAATGTCGCGACCGTGAAGTTGCACCGGTGGACCATGTTCAGGAAC
TGTTTGCTCCACGCGAAATGACCTGCTTTGCAAGCGCTTTCCACTCTTGA
CTTGCTGCTCACGGATAATCGGAGCCGAGTGCTACGGTGAATACCAGCCA
CCGATGGACGTGGCTTCATTTTTTTTTTTCTCATCTCTTCGATGCGTGCC
TATTGTGCAGTATTGCCGAGTGTATTGGCGCAATGCACTCCACCCGTATC
AAGAGAGGTAATTGTGGCGCAATTTTTTCGACAAAACATCCCACCCCCCC
CCCCCTCCCGCTGCTGCTTCCACTTCGATCGAAACACGTGTGTGTGCTAT
CATGCCCACTTTTAGTACGATGGAGACAAACTCACCACGGTCGACGAACA
AGCACATGCTTTCGTAGCCTGCATCATGGTGGGCACGTAATCATGTAACT
GTAGGAGGAACTGCTGTTGCGGTTGGCATCGTGAGCGTTGTGTATCTTGC
CTGTTGGTCTCGACGTCATGCTGGTAGTCTCCTGAAATGTGCCCTCGAAA
TGACCTCTGCTGTGTTCCGCTATGCCTTCTCACGCTCAAGTCCCTTCTTC
CTGGAGACCTTTGGACCGTTTCTGCCCTCAACAACCAGCATGGTGCCTGC
ATGGAACGCTTACTTATTCGCCCTTCTACGTTGCTTCCCTCTGTTGCGCG
TGCCTGCACCCAGCTGCTGGACCAGTTCCTGGTGGCCGACGAGTTCAAAC
TGCGGTATATCCTCTTCCCGCAGGCGTTCGGGGTGATTTACATTTTGTTC
AACGTGGGCTGGTATTATCTCGCCCCGGACGATGACCGACTGATTTACAT
CATCCTCGACTGGGAAGACAACACTCTAGTGGCGTGCATCTATGCCTTTG
GCGCGATTTTGGTTGGCGCACCGCTCTTTGGGTTGTTCCACTTAGGCATC
TTTAGGTAAGAATTAGGTATTTAACAGCATCCTATTTCACCCCGGTCTTC
TTCCCTTCACCCCAAGTCGCGCAGACGTCGTATTAATAGTACATTGTCAA
CATGTCGCATGTCTCGAGTTTCGCCAGCTCTTGAAGCAGTCCCGATTATT
ACTTCTCACTTTTTTTCCTCGCACCCTGAAGGACCCGGCCCCCTTTCTCT
TCCTCAGCCTTCTACGCGGGTAGGGTCGAGCGTCGAACGGCATTTATATG
TGAAGGGTGACCTCGTGGTAGTTCGCTCGCCTGGCTAGCCCTGATATTAT
TCCCTTCTCGCCAAACCCGTCAAGGTTCACACCACAAAAGTCACCGGTGT
ATCCCACGCTGCATCGTTCTCGAGTCCTTGTTTGCAATACCGGGACTGAG
CTAGCGGAACAGGGTGTGTCGGACACCTTTCGTTGTTTGGTTCGATAGAT
GTTGAGTCGACATCCGAGTTGAACACATGTTCCAACGGAGGGAGGAAAGC
CCTTGGTTGCCAGCACCGGGGGCGCCTTCCCACCTCGATGCCACCATGCT
CGTCATCATGAATCGGGGTCTATACGCCGCCAAAACTTGTCTGGGTTGCA
CCCGCTTGGTCTTGAAAACAGCCCAATTTGGCCATCATCCCCCAAACAAC
GATCACGTTTTCCCATCCCCGCGAAAAACAGACTCCGCGAAAAAGTCTAC
CAGAATCGTCTAGCCAGAAGCTCGAAGGCCGCAGCGGAGGCCCAAGCGAT
CGAATTGAAGGAGAGCGACCAAGCTTGAGATCTTGCTGTTTGATGGGCGG
TTGAGGAGCTCGGCAGGAAGGAGTGGGTTGTTGTAGTATAAGGCAAGAGA
CTGCTGACACGGACGAGCGGGTTGCAGTAGTGGACTATTTTACAGCGAAT
GGTAGAGTGCTGAAGGAAGACCTTGTTCGTGGAAGGCGGTAGAAAAATCG
TAGTATGATGCGGGGACGCAGCATCAACGTCCCCGGAAAAAGCCGCAGGG
TAGGGCACACTCGCAAAATTGCTAGGAAAACAACTCGGAGGACAGGTGGC
CCGCCGAGGAGCGTCGGGCTAGCAGAAGTGGCCTAACCCTCGGGAAAGTG
GTGGGCTTGCAGGAGGGGAGCTGTTTTGCCAGAATCTGGCCAGGGAGAAC
CAGCTAGATCCCGTACATCTGCCACCGATAGATGTGGTATGCAAACATAT
TTTTAAACATTCGAGGCTGATCCGTGGTTCACCCTCGAAGAATTGGCGCT
AGAATGAGATTTTTCGACACGTCCGAACATGACCGGCACCTCCACATGCA
AAGTAGAAACGTGGTGATTACTTGACGTTGTATTCTATACTCGATTTTTG
TGTTCTATAAATTATGAAGTATTACTATCAGCGGGAATTGCTACAGGCTT
GACCGAGGTGTTCTGGAGTGCAATTTTTTTGTGTGTTTGGAGAATTTCCT
TACCTGACGGTTCTACCAACCGGAGCGACGGCGAAAGTTGTCGTACACAC
CCACAAGGTTAAACGTTTTGTGTGCCGGTATGAGGTGTTACCCTGGAGTA
ACGTGAGACAGTAGCTCTTCGTAGCGCCAAAGGGCGTTCAGCTCTATTTA
GCATGCTTTTTTCTTGAATCAAAAACGAAGAGATTTGTCGCGTTTGCGTT
TCACGTATTTTGCTTGGACACATGCATTGGTGTCTGTAGATGATATATAT
ATATATTTTGTGTTGTGTTGTTTTCAAGTAGACCAGTATTATAGGAGGGA
CCACCATTCCCGTATGGTCGTGTAATCCTCCTCCTTTTGTGGTCATATGG
TGCCGTCGCAGTTTTCCGTCTGTGGTCACGGACGATTCGACAGCACTTGT
CGTACACCGCCGCCCTCAAGAAGAAGTTCACCACCGGCGGTTTCTTTTCG
CTCGGGGGGGAGGGGCAAGAACGAGTGTTGTAGCACGATGATTTGTTGGC
GAGTCAAGTGTAGCATGACGATCGTTTTGGGTCCTCTCGTCGTCTCAGGC
GCTGCAAGTGTCTGTCTGTGTGGTCGACCAACAGCGTAAGAACCGTCGGT
ACGTTCATAAGGTTGGGGTGTGCTTGGCATGATGCTTGATGGGTGCCTCC
CCGCAAAAAAGGGCACGTGGAGATGCGACCTAGCCCACCATGATGCTGAC
TTGCATGTTGTGCGCGTGCTCGATGTTGTTGCGTCTTAGTTTGAGTTGTA
TCTTGCGAAGAAAGCCCTTCGTGCGCCCCTCGAGGGTGGGGAGTGTGGTT
GGCCCGGTCGGCCAAGAGACAAAAGTGCACCATCAACAGAACACCCGCCT
GGCTTGGTAACGTGTGAAGCTTGTTACTGTTTTGTTGTCCGGTTCGCTTC
CGAGTTGAGTACTGGGCTGTGGAGCCAGTTGCTGCCACCTGTGGACTAGA
CTCTGTGTGTACTCTGTAGAATAGGGTGGAGTACAAATGTGGGAGGTACA
GCAGTACACCACATAAGTTTTCGAGAGTGTGTGGTGGAACCTCCTGGAGT
CTCATGATCAGCTGTGTCCCGAGTTCGTCGCGGGACGCAAGATTACTTTC
GAGTTTTTTTTTAATTTATTATGAGTCAAATTATGGTGCGCGTCGCGTTA
GCTTGACGTTCTTATGGAAATGGATCATGAATGGATGGCACAGGCGACCA
CGGGAATAGACCGCGTCGAGTGTTGGATCAGTACGGACACACGAAGGTAC
TGGGTAGAGAGAGATCAGGACGGTTCTTAATGTGAAGACAAC
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Coding sequence (CDS) from alignment at Ecto-sp13_S_contig9827:1397..5638-

>mRNA_Ecto-sp13_S_contig9827.21474.1 ID=mRNA_Ecto-sp13_S_contig9827.21474.1|Name=mRNA_Ecto-sp13_S_contig9827.21474.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=CDS|length=342bp|location=Sequence derived from alignment at Ecto-sp13_S_contig9827:1397..5638- (Ectocarpus species13 EcNAP12_S_4_19m)
ATGCTGCTGGACCAGTTCCTGGTGGCCGACGAGTTCAAACTGCGGTATAT
CCTCTTCCCGCAGGCGTTCGGGGTGATTTACATTTTGTTCAACGTGGGCT
GGTATTATCTCGCCCCGGACGATGACCGACTGATTTACATCATCCTCGAC
TGGGAAGACAACACTCTAGTGGCGTGCATCTATGCCTTTGGCGCGATTTT
GGTTGGCGCACCGCTCTTTGGGTTGTTCCACTTAGGCATCTTTAGACTCC
GCGAAAAAGTCTACCAGAATCGTCTAGCCAGAAGCTCGAAGGCCGCAGCG
GAGGCCCAAGCGATCGAATTGAAGGAGAGCGACCAAGCTTGA
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