mRNA_Ecto-sp13_S_contig97856.21436.1 (mRNA) Ectocarpus species13 EcNAP12_S_4_19m

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_Ecto-sp13_S_contig97856.21436.1
Unique NamemRNA_Ecto-sp13_S_contig97856.21436.1
TypemRNA
OrganismEctocarpus species13 EcNAP12_S_4_19m (Ectocarpus species13 EcNAP12_S_4_19m)
Homology
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BLAST of mRNA_Ecto-sp13_S_contig97856.21436.1 vs. uniprot
Match: D8LPJ9_ECTSI (Ribonucleoside-diphosphate reductase n=2 Tax=root TaxID=1 RepID=D8LPJ9_ECTSI)

HSP 1 Score: 283 bits (725), Expect = 3.660e-89
Identity = 133/140 (95.00%), Postives = 139/140 (99.29%), Query Frame = 1 
Query:    1 RKGSFAMYLEMWHADIFEFLDLKKNNGNELMRARDLFYGLWVSDLFMHRVKADGNWTLLSPSDAPDLIDLHGADFDQAYKKYESNGIGKTIKARTLFAKVVESQIETGGPYYLFKDACNAKSNQQHLGTIRSSNLCAEVV 420
            RKGSFAMYLEMWHADI EFLDLKKNNGNELMRARDLFYGLWVSDLFMHRVKADG+WTLLSPSDAPDLIDLHGADFD+AY+KYES+GIGKTIKARTLFAKVVESQIETGGPYYLFKD+CNAKSNQQHLGTIRSSNLCAEV+
Sbjct:  286 RKGSFAMYLEMWHADILEFLDLKKNNGNELMRARDLFYGLWVSDLFMHRVKADGDWTLLSPSDAPDLIDLHGADFDRAYEKYESDGIGKTIKARTLFAKVVESQIETGGPYYLFKDSCNAKSNQQHLGTIRSSNLCAEVI 425
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BLAST of mRNA_Ecto-sp13_S_contig97856.21436.1 vs. uniprot
Match: A0A2T4WP70_9BACT (Ribonucleoside-diphosphate reductase subunit alpha (Fragment) n=1 Tax=Bacteroidetes bacterium TaxID=1898104 RepID=A0A2T4WP70_9BACT)

HSP 1 Score: 209 bits (531), Expect = 5.790e-62
Identity = 96/141 (68.09%), Postives = 115/141 (81.56%), Query Frame = 1 
Query:    1 RKGSFAMYLEMWHADIFEFLDLKKNNGNELMRARDLFYGLWVSDLFMHRVKADGNWTLLSPSDAPDLIDLHGADFDQAYKKYESNGIG-KTIKARTLFAKVVESQIETGGPYYLFKDACNAKSNQQHLGTIRSSNLCAEVV 420
            RKGSFAMY+E WHADIF+FLDLKKN+G E MRARDLFY +W+ DLFM RVKAD  WTL+ P++ P L D+H  +F+  Y KYES G G KTIKAR L+ K++ESQIETG PY L+KDA N+KSNQQ+LGTIRSSNLC E++
Sbjct:   64 RKGSFAMYVEPWHADIFDFLDLKKNHGKEEMRARDLFYAMWIPDLFMERVKADSTWTLMCPNECPGLFDVHSEEFNTLYHKYESEGKGRKTIKARELWGKILESQIETGTPYMLYKDAANSKSNQQNLGTIRSSNLCTEII 204
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BLAST of mRNA_Ecto-sp13_S_contig97856.21436.1 vs. uniprot
Match: A0A6T9KEA0_9EUKA (Hypothetical protein n=1 Tax=Haptolina ericina TaxID=156174 RepID=A0A6T9KEA0_9EUKA)

HSP 1 Score: 196 bits (497), Expect = 1.840e-60
Identity = 90/141 (63.83%), Postives = 111/141 (78.72%), Query Frame = 1 
Query:    1 RKGSFAMYLEMWHADIFEFLDLKKNNGNELMRARDLFYGLWVSDLFMHRVKADGNWTLLSPSDAPDLIDLHGADFDQAYKKYESNGIGK-TIKARTLFAKVVESQIETGGPYYLFKDACNAKSNQQHLGTIRSSNLCAEVV 420
            RKG+FA+YLE WHAD+F FL+LKKN+G E  RARDLFY LWV DLFM RV+A G W+L  P +AP L D++G  FD+ Y++YE  G  K T++A+ L+ K+VESQ+ETG PY L+KDACN+KSNQQHLG IR SNLC EVV
Sbjct:   54 RKGAFAIYLEPWHADVFAFLELKKNHGKEESRARDLFYALWVPDLFMRRVEAGGEWSLFCPDEAPGLADVYGKAFDELYERYEREGRAKRTVQAQQLWFKIVESQMETGTPYMLYKDACNSKSNQQHLGAIRGSNLCTEVV 194
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BLAST of mRNA_Ecto-sp13_S_contig97856.21436.1 vs. uniprot
Match: A0A2D7SPF7_9FLAO (Ribonucleoside-diphosphate reductase n=1 Tax=Flavobacteriales bacterium TaxID=2021391 RepID=A0A2D7SPF7_9FLAO)

HSP 1 Score: 207 bits (528), Expect = 2.480e-60
Identity = 95/141 (67.38%), Postives = 117/141 (82.98%), Query Frame = 1 
Query:    1 RKGSFAMYLEMWHADIFEFLDLKKNNGNELMRARDLFYGLWVSDLFMHRVKADGNWTLLSPSDAPDLIDLHGADFDQAYKKYESNGIG-KTIKARTLFAKVVESQIETGGPYYLFKDACNAKSNQQHLGTIRSSNLCAEVV 420
            RKGSFA+Y+E WHADIFEFLDL+KN+G E MRARDLFY LW  DLFM RV+ DGNWTL+ P++ P L +++G +F+  Y+KYES G G KTIKAR L+ K++ESQIETG PY LFKD+CN KSNQ++LGTIRSSNLC E++
Sbjct:  292 RKGSFAIYIEPWHADIFEFLDLRKNHGKEEMRARDLFYALWTPDLFMERVEKDGNWTLMCPNECPGLANIYGDEFNDKYEKYESEGKGRKTIKARELWDKIIESQIETGTPYMLFKDSCNIKSNQKNLGTIRSSNLCTEIL 432
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BLAST of mRNA_Ecto-sp13_S_contig97856.21436.1 vs. uniprot
Match: A0A506PQP7_9FLAO (Ribonucleoside-diphosphate reductase n=1 Tax=Paucihalobacter ruber TaxID=2567861 RepID=A0A506PQP7_9FLAO)

HSP 1 Score: 207 bits (528), Expect = 3.360e-60
Identity = 95/141 (67.38%), Postives = 116/141 (82.27%), Query Frame = 1 
Query:    1 RKGSFAMYLEMWHADIFEFLDLKKNNGNELMRARDLFYGLWVSDLFMHRVKADGNWTLLSPSDAPDLIDLHGADFDQAYKKYESNGIG-KTIKARTLFAKVVESQIETGGPYYLFKDACNAKSNQQHLGTIRSSNLCAEVV 420
            RKGSFA+Y+E WHADIF+FLDLKKN+G E MRARDLFY +W+SDLFM RV+ DG+WTL+ P++ P L D+H   F+  Y KYE+ G G KTIKAR L+ K++ESQIETG PY L+KDACN KSNQQ+LGTIRSSNLC E++
Sbjct:  292 RKGSFAIYIETWHADIFDFLDLKKNHGKEEMRARDLFYAMWISDLFMKRVQEDGHWTLMCPNECPGLADVHSEAFETLYTKYEAEGKGRKTIKARELWEKILESQIETGTPYMLYKDACNRKSNQQNLGTIRSSNLCTEIL 432
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BLAST of mRNA_Ecto-sp13_S_contig97856.21436.1 vs. uniprot
Match: A0A401XMX1_9FLAO (Ribonucleoside-diphosphate reductase n=1 Tax=Thermaurantimonas aggregans TaxID=2173829 RepID=A0A401XMX1_9FLAO)

HSP 1 Score: 207 bits (528), Expect = 4.260e-60
Identity = 94/141 (66.67%), Postives = 117/141 (82.98%), Query Frame = 1 
Query:    1 RKGSFAMYLEMWHADIFEFLDLKKNNGNELMRARDLFYGLWVSDLFMHRVKADGNWTLLSPSDAPDLIDLHGADFDQAYKKYESNGIGK-TIKARTLFAKVVESQIETGGPYYLFKDACNAKSNQQHLGTIRSSNLCAEVV 420
            RKGSFA+YLE WHADIF+FLDLKKN+G E +RARDLFY LW+ DLFM RV+ADGNWTL+ P++ P L D +G  F++ Y++YE  G G+ TIKAR L+ K++ESQIETG PY L+KDACN KSNQ++LGTIRSSNLC E++
Sbjct:  292 RKGSFAIYLEPWHADIFDFLDLKKNHGKEELRARDLFYALWIPDLFMKRVEADGNWTLMCPNECPGLYDTYGKKFEELYEQYEREGRGRRTIKARELWQKIIESQIETGTPYMLYKDACNEKSNQKNLGTIRSSNLCTEII 432
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BLAST of mRNA_Ecto-sp13_S_contig97856.21436.1 vs. uniprot
Match: A0A812YHU2_9DINO (Ribonucleoside-diphosphate reductase n=1 Tax=Symbiodinium sp. CCMP2456 TaxID=1519616 RepID=A0A812YHU2_9DINO)

HSP 1 Score: 209 bits (532), Expect = 4.970e-60
Identity = 97/141 (68.79%), Postives = 117/141 (82.98%), Query Frame = 1 
Query:    1 RKGSFAMYLEMWHADIFEFLDLKKNNGNELMRARDLFYGLWVSDLFMHRVKADGNWTLLSPSDAPDLIDLHGADFDQAYKKYESNGIG-KTIKARTLFAKVVESQIETGGPYYLFKDACNAKSNQQHLGTIRSSNLCAEVV 420
            RKGSFA+YLE WHADIF+FLDLKKN+G E MRARDLFY LW  DLFM RVK DG WTL+ P++ P L D+H  +F++ Y+KYE+ G G KTIKAR L++K++ESQIETG PY L+KDA NAKSNQQ+LGTIRSSNLC E++
Sbjct:  740 RKGSFAIYLEPWHADIFDFLDLKKNHGKEEMRARDLFYALWTPDLFMKRVKEDGMWTLMDPNECPGLCDVHSEEFNKLYEKYEAEGKGRKTIKARDLWSKIMESQIETGTPYMLYKDAANAKSNQQNLGTIRSSNLCTEII 880
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BLAST of mRNA_Ecto-sp13_S_contig97856.21436.1 vs. uniprot
Match: M3HM96_CANMX (Ribonucleoside-diphosphate reductase n=1 Tax=Candida maltosa (strain Xu316) TaxID=1245528 RepID=M3HM96_CANMX)

HSP 1 Score: 207 bits (526), Expect = 6.040e-60
Identity = 89/140 (63.57%), Postives = 116/140 (82.86%), Query Frame = 1 
Query:    1 RKGSFAMYLEMWHADIFEFLDLKKNNGNELMRARDLFYGLWVSDLFMHRVKADGNWTLLSPSDAPDLIDLHGADFDQAYKKYESNGIGKTIKARTLFAKVVESQIETGGPYYLFKDACNAKSNQQHLGTIRSSNLCAEVV 420
            R G+FA+Y+E WHADIFE LD++KN+G+E +R RDLFYGLWV DLFM RVK +GNWTL SP++AP L D++G DF++ Y+KYE  G+G+ +KA  L+  ++ESQ ETGGPY L+KDACN KSNQ++LGTI+SSNLC E+V
Sbjct:  291 RPGAFAIYVEPWHADIFEILDMRKNHGSEELRTRDLFYGLWVPDLFMKRVKENGNWTLFSPNEAPGLSDVYGEDFEELYQKYEQQGLGREVKAHKLWLSILESQTETGGPYMLYKDACNTKSNQKNLGTIKSSNLCCEIV 430
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BLAST of mRNA_Ecto-sp13_S_contig97856.21436.1 vs. uniprot
Match: A0A2D5HQE0_9FLAO (Ribonucleoside-diphosphate reductase n=4 Tax=unclassified Flavobacteriaceae TaxID=61432 RepID=A0A2D5HQE0_9FLAO)

HSP 1 Score: 206 bits (523), Expect = 1.550e-59
Identity = 94/141 (66.67%), Postives = 117/141 (82.98%), Query Frame = 1 
Query:    1 RKGSFAMYLEMWHADIFEFLDLKKNNGNELMRARDLFYGLWVSDLFMHRVKADGNWTLLSPSDAPDLIDLHGADFDQAYKKYESNGIG-KTIKARTLFAKVVESQIETGGPYYLFKDACNAKSNQQHLGTIRSSNLCAEVV 420
            RKGSFA+Y+E WHADIF+FL+LKKN+G E MRARDLFY +W  DLFM RV+ D NWTL+ P++ PDL +LHG +FD+ Y+KYES G G KTIKAR L+ K++ESQIETG PY L+KD+ N KSNQ++LGTIRSSNLC E++
Sbjct:  292 RKGSFAIYVEPWHADIFDFLELKKNHGKEEMRARDLFYAMWTPDLFMKRVEEDSNWTLMCPNECPDLYNLHGDEFDELYQKYESEGRGRKTIKARELWEKILESQIETGTPYMLYKDSANRKSNQKNLGTIRSSNLCTEIL 432
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BLAST of mRNA_Ecto-sp13_S_contig97856.21436.1 vs. uniprot
Match: A0A2E8DJR3_9FLAO (Ribonucleoside-diphosphate reductase (Fragment) n=1 Tax=Flavobacteriaceae bacterium TaxID=1871037 RepID=A0A2E8DJR3_9FLAO)

HSP 1 Score: 202 bits (514), Expect = 1.680e-59
Identity = 93/141 (65.96%), Postives = 115/141 (81.56%), Query Frame = 1 
Query:    1 RKGSFAMYLEMWHADIFEFLDLKKNNGNELMRARDLFYGLWVSDLFMHRVKADGNWTLLSPSDAPDLIDLHGADFDQAYKKYESNGIG-KTIKARTLFAKVVESQIETGGPYYLFKDACNAKSNQQHLGTIRSSNLCAEVV 420
            RKGSFAMYLE WHADIF+FLDLKKN+G E MRARDLFY +W+SDLFM RV+ DG WTL+ P++ P + ++H  +F+  Y KYE+ G G KTIKAR L+ K++ESQIETG PY L+KDA N KSNQ++LGTIRSSNLC E++
Sbjct:  292 RKGSFAMYLETWHADIFDFLDLKKNHGKEEMRARDLFYAMWISDLFMKRVQEDGKWTLMCPNECPGMDEVHSDEFEALYLKYEAEGKGRKTIKARELWEKILESQIETGTPYMLYKDAANRKSNQKNLGTIRSSNLCTEIM 432
The following BLAST results are available for this feature:
BLAST of mRNA_Ecto-sp13_S_contig97856.21436.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
D8LPJ9_ECTSI3.660e-8995.00Ribonucleoside-diphosphate reductase n=2 Tax=root ... [more]
A0A2T4WP70_9BACT5.790e-6268.09Ribonucleoside-diphosphate reductase subunit alpha... [more]
A0A6T9KEA0_9EUKA1.840e-6063.83Hypothetical protein n=1 Tax=Haptolina ericina Tax... [more]
A0A2D7SPF7_9FLAO2.480e-6067.38Ribonucleoside-diphosphate reductase n=1 Tax=Flavo... [more]
A0A506PQP7_9FLAO3.360e-6067.38Ribonucleoside-diphosphate reductase n=1 Tax=Pauci... [more]
A0A401XMX1_9FLAO4.260e-6066.67Ribonucleoside-diphosphate reductase n=1 Tax=Therm... [more]
A0A812YHU2_9DINO4.970e-6068.79Ribonucleoside-diphosphate reductase n=1 Tax=Symbi... [more]
M3HM96_CANMX6.040e-6063.57Ribonucleoside-diphosphate reductase n=1 Tax=Candi... [more]
A0A2D5HQE0_9FLAO1.550e-5966.67Ribonucleoside-diphosphate reductase n=4 Tax=uncla... [more]
A0A2E8DJR3_9FLAO1.680e-5965.96Ribonucleoside-diphosphate reductase (Fragment) n=... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
Ecto-sp13_S_contig97856contigEcto-sp13_S_contig97856:2..421 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Ectocarpus species13 EcNAP12_S_4_19m OGS1.02022-07-08
Properties
Property NameValue
Taxonomic scopeEukaryota
Stop0
Start1
Seed ortholog score270.8
Seed ortholog evalue3.5e-70
Seed eggNOG ortholog2880.D8LPJ9
Preferred nameRNR1
Model size420
KEGG rclassRC00613,RC01003
KEGG koko:K10807
KEGG ReactionR02017,R02019,R02024,R08363,R08364,R11893
KEGG Pathwayko00230,ko00240,ko00480,ko00983,ko01100,map00230,map00240,map00480,map00983,map01100
KEGG ModuleM00053
Hectar predicted targeting categoryother localisation
GOsGO:0000166,GO:0003674,GO:0003824,GO:0004550,GO:0004748,GO:0005488,GO:0005524,GO:0005575,GO:0005622,GO:0005623,GO:0005634,GO:0005737,GO:0005739,GO:0005829,GO:0005971,GO:0006139,GO:0006165,GO:0006213,GO:0006220,GO:0006221,GO:0006235,GO:0006240,GO:0006725,GO:0006753,GO:0006793,GO:0006796,GO:0006807,GO:0008144,GO:0008150,GO:0008152,GO:0009058,GO:0009116,GO:0009117,GO:0009119,GO:0009132,GO:0009133,GO:0009138,GO:0009139,GO:0009141,GO:0009142,GO:0009147,GO:0009148,GO:0009165,GO:0009185,GO:0009186,GO:0009189,GO:0009193,GO:0009196,GO:0009197,GO:0009200,GO:0009202,GO:0009211,GO:0009212,GO:0009218,GO:0009219,GO:0009221,GO:0009259,GO:0009262,GO:0009263,GO:0009265,GO:0009394,GO:0009987,GO:0016301,GO:0016310,GO:0016491,GO:0016725,GO:0016728,GO:0016740,GO:0016772,GO:0016776,GO:0017076,GO:0018130,GO:0019205,GO:0019438,GO:0019637,GO:0019692,GO:0019693,GO:0030554,GO:0032553,GO:0032555,GO:0032559,GO:0032991,GO:0034641,GO:0034654,GO:0035639,GO:0036094,GO:0043167,GO:0043168,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0044237,GO:0044238,GO:0044249,GO:0044271,GO:0044281,GO:0044424,GO:0044444,GO:0044445,GO:0044464,GO:0046062,GO:0046075,GO:0046131,GO:0046385,GO:0046483,GO:0046704,GO:0046939,GO:0051063,GO:0055086,GO:0055114,GO:0061731,GO:0071704,GO:0072527,GO:0072528,GO:0090407,GO:0097159,GO:0097367,GO:1901135,GO:1901137,GO:1901265,GO:1901293,GO:1901360,GO:1901362,GO:1901363,GO:1901564,GO:1901566,GO:1901576,GO:1901657,GO:1902494,GO:1990204
Exons1
EggNOG free text desc.ribonucleoside-diphosphate reductase activity
EggNOG OGsCOG0209@1,KOG1112@2759
EC1.17.4.1
Cds size402
COG Functional cat.F
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko00002,ko01000,ko03400
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1681464242.342528-CDS-Ecto-sp13_S_contig97856:1..4031681464242.342528-CDS-Ecto-sp13_S_contig97856:1..403Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig97856 2..403 -


The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1681464242.3539824-UTR-Ecto-sp13_S_contig97856:403..4211681464242.3539824-UTR-Ecto-sp13_S_contig97856:403..421Ectocarpus species13 EcNAP12_S_4_19mUTREcto-sp13_S_contig97856 404..421 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_Ecto-sp13_S_contig97856.21436.1prot_Ecto-sp13_S_contig97856.21436.1Ectocarpus species13 EcNAP12_S_4_19mpolypeptideEcto-sp13_S_contig97856 2..403 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_Ecto-sp13_S_contig97856.21436.1

>prot_Ecto-sp13_S_contig97856.21436.1 ID=prot_Ecto-sp13_S_contig97856.21436.1|Name=mRNA_Ecto-sp13_S_contig97856.21436.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=polypeptide|length=134bp
MYLEMWHADIFEFLDLKKNNGNELMRARDLFYGLWVSDLFMHRVKADGNW
TLLSPSDAPDLIDLHGADFDQAYKKYESNGIGKTIKARTLFAKVVESQIE
TGGPYYLFKDACNAKSNQQHLGTIRSSNLCAEVV
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mRNA from alignment at Ecto-sp13_S_contig97856:2..421-

Legend: UTRCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_Ecto-sp13_S_contig97856.21436.1 ID=mRNA_Ecto-sp13_S_contig97856.21436.1|Name=mRNA_Ecto-sp13_S_contig97856.21436.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=mRNA|length=420bp|location=Sequence derived from alignment at Ecto-sp13_S_contig97856:2..421- (Ectocarpus species13 EcNAP12_S_4_19m)
CGAAAGGGGTCGTTCGCCATGTATTTGGAAATGTGGCACGCCGACATATT
CGAGTTTCTGGACTTGAAAAAGAACAACGGCAACGAGCTCATGAGAGCGC
GCGACCTCTTCTACGGGCTGTGGGTCTCCGATTTGTTCATGCATCGCGTC
AAGGCCGACGGCAATTGGACGCTTCTTTCTCCGTCCGACGCCCCGGACCT
CATCGATCTCCACGGTGCCGACTTCGACCAAGCCTACAAGAAGTACGAAT
CCAACGGCATCGGCAAGACAATCAAGGCTCGGACACTTTTTGCCAAGGTG
GTGGAGTCTCAGATAGAGACAGGGGGGCCGTATTATTTGTTCAAAGACGC
GTGCAACGCCAAGAGTAACCAACAGCATCTCGGTACCATCCGCTCGTCAA
ATCTCTGTGCAGAGGTGGTT
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Coding sequence (CDS) from alignment at Ecto-sp13_S_contig97856:2..421-

>mRNA_Ecto-sp13_S_contig97856.21436.1 ID=mRNA_Ecto-sp13_S_contig97856.21436.1|Name=mRNA_Ecto-sp13_S_contig97856.21436.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=CDS|length=402bp|location=Sequence derived from alignment at Ecto-sp13_S_contig97856:2..421- (Ectocarpus species13 EcNAP12_S_4_19m)
ATGTATTTGGAAATGTGGCACGCCGACATATTCGAGTTTCTGGACTTGAA
AAAGAACAACGGCAACGAGCTCATGAGAGCGCGCGACCTCTTCTACGGGC
TGTGGGTCTCCGATTTGTTCATGCATCGCGTCAAGGCCGACGGCAATTGG
ACGCTTCTTTCTCCGTCCGACGCCCCGGACCTCATCGATCTCCACGGTGC
CGACTTCGACCAAGCCTACAAGAAGTACGAATCCAACGGCATCGGCAAGA
CAATCAAGGCTCGGACACTTTTTGCCAAGGTGGTGGAGTCTCAGATAGAG
ACAGGGGGGCCGTATTATTTGTTCAAAGACGCGTGCAACGCCAAGAGTAA
CCAACAGCATCTCGGTACCATCCGCTCGTCAAATCTCTGTGCAGAGGTGG
TT
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