mRNA_Ecto-sp13_S_contig92814.20874.1 (mRNA) Ectocarpus species13 EcNAP12_S_4_19m

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_Ecto-sp13_S_contig92814.20874.1
Unique NamemRNA_Ecto-sp13_S_contig92814.20874.1
TypemRNA
OrganismEctocarpus species13 EcNAP12_S_4_19m (Ectocarpus species13 EcNAP12_S_4_19m)
Homology
BLAST of mRNA_Ecto-sp13_S_contig92814.20874.1 vs. uniprot
Match: A0A2P6TYS0_CHLSO (Monodehydroascorbate reductase n=1 Tax=Chlorella sorokiniana TaxID=3076 RepID=A0A2P6TYS0_CHLSO)

HSP 1 Score: 55.5 bits (132), Expect = 1.210e-7
Identity = 25/49 (51.02%), Postives = 32/49 (65.31%), Query Frame = 1
Query:    1 RFLDISWKFYGFARGEVCVLGMETFPMTRTFGAFWLHGGKIVGAFLEVG 147
            R  ++SW+FYG + GEV   G  +      FGAFW+ GGK+VGAFLE G
Sbjct:  348 RVFNLSWQFYGLSEGEVVQFGDVS---AGKFGAFWVQGGKVVGAFLESG 393          
BLAST of mRNA_Ecto-sp13_S_contig92814.20874.1 vs. uniprot
Match: A0A2P5B703_PARAD (FAD/NAD(P)-binding domain containing protein n=2 Tax=Cannabaceae TaxID=3481 RepID=A0A2P5B703_PARAD)

HSP 1 Score: 53.5 bits (127), Expect = 5.820e-7
Identity = 24/51 (47.06%), Postives = 33/51 (64.71%), Query Frame = 1
Query:    1 RFLDISWKFYGFARGEVCVLGMETFPMTRT--FGAFWLHGGKIVGAFLEVG 147
            R  D+SWKFYG   GE  + G    P+++   FG++W+H GK+ GAFLE G
Sbjct:  458 RIFDLSWKFYGDNVGETVIFGNHN-PLSKEPKFGSYWIHMGKVKGAFLEGG 507          
BLAST of mRNA_Ecto-sp13_S_contig92814.20874.1 vs. uniprot
Match: A0A0A9DAU7_ARUDO (Pyr_redox_2 domain-containing protein n=1 Tax=Arundo donax TaxID=35708 RepID=A0A0A9DAU7_ARUDO)

HSP 1 Score: 51.6 bits (122), Expect = 1.840e-6
Identity = 24/49 (48.98%), Postives = 30/49 (61.22%), Query Frame = 1
Query:    1 RFLDISWKFYGFARGEVCVLGMETFPMTRTFGAFWLHGGKIVGAFLEVG 147
            R   +SW+FYG   GE    G +  P +  FGAFW+ GG+I GAFLE G
Sbjct:   76 RVFALSWRFYGDNAGEAVHFG-DFSPSSPKFGAFWVRGGRIAGAFLEGG 123          
BLAST of mRNA_Ecto-sp13_S_contig92814.20874.1 vs. uniprot
Match: UPI000901E7CA (monodehydroascorbate reductase-like n=2 Tax=Ipomoea TaxID=4119 RepID=UPI000901E7CA)

HSP 1 Score: 51.6 bits (122), Expect = 2.780e-6
Identity = 22/50 (44.00%), Postives = 30/50 (60.00%), Query Frame = 1
Query:    1 RFLDISWKFYGFARGEVCVLG-METFPMTRTFGAFWLHGGKIVGAFLEVG 147
            R  D+SW+FYG   GE  + G  +       FG +W+H GK++GAFLE G
Sbjct:  350 RAFDLSWQFYGDNVGETVLFGDNDPTSANHKFGQYWIHNGKVIGAFLEGG 399          
BLAST of mRNA_Ecto-sp13_S_contig92814.20874.1 vs. uniprot
Match: A0A8K0HIV5_9ROSA (Uncharacterized protein n=1 Tax=Rhamnella rubrinervis TaxID=2594499 RepID=A0A8K0HIV5_9ROSA)

HSP 1 Score: 51.2 bits (121), Expect = 3.820e-6
Identity = 26/52 (50.00%), Postives = 32/52 (61.54%), Query Frame = 1
Query:    1 RFLDISWKFYGFARGEVCVLGMETFPMTRT---FGAFWLHGGKIVGAFLEVG 147
            R+ D+SW FYG   GE  V   +T   T T   FG +W+H GK+VGAFLE G
Sbjct:  442 RYFDLSWCFYGDNVGEP-VFFKDTVNATSTKPRFGTYWIHNGKVVGAFLEGG 492          
BLAST of mRNA_Ecto-sp13_S_contig92814.20874.1 vs. uniprot
Match: A0A6P6FMN7_ZIZJJ (monodehydroascorbate reductase, seedling isozyme-like isoform X1 n=4 Tax=Ziziphus jujuba TaxID=326968 RepID=A0A6P6FMN7_ZIZJJ)

HSP 1 Score: 49.7 bits (117), Expect = 1.340e-5
Identity = 26/56 (46.43%), Postives = 32/56 (57.14%), Query Frame = 1
Query:    1 RFLDISWKFYGFARGE-VCVLGMETFPMTRT------FGAFWLHGGKIVGAFLEVG 147
            RF D+SW FYG   GE V    ++    TR       FG +W+H GK+VGAFLE G
Sbjct:  477 RFFDLSWVFYGDNVGEPVLFQDIQDNSTTRPSSTKPKFGTYWIHNGKVVGAFLEGG 532          
BLAST of mRNA_Ecto-sp13_S_contig92814.20874.1 vs. uniprot
Match: MDAR1_ARATH (Monodehydroascorbate reductase 1, peroxisomal n=32 Tax=Brassicaceae TaxID=3700 RepID=MDAR1_ARATH)

HSP 1 Score: 49.3 bits (116), Expect = 1.830e-5
Identity = 24/51 (47.06%), Postives = 33/51 (64.71%), Query Frame = 1
Query:    1 RFLDISWKFYGFARGEVCVLGME--TFPMTRTFGAFWLHGGKIVGAFLEVG 147
            R  D+SW+FYG   G+  + G    + P  R FGA+W+ GGK+VGAF+E G
Sbjct:  350 RSFDLSWQFYGDNVGDSVLFGDSNPSNPKPR-FGAYWVQGGKVVGAFMEGG 399          
BLAST of mRNA_Ecto-sp13_S_contig92814.20874.1 vs. uniprot
Match: A9TN13_PHYPA (Predicted protein n=1 Tax=Physcomitrium patens TaxID=3218 RepID=A9TN13_PHYPA)

HSP 1 Score: 49.3 bits (116), Expect = 1.830e-5
Identity = 22/49 (44.90%), Postives = 31/49 (63.27%), Query Frame = 1
Query:    1 RFLDISWKFYGFARGEVCVLGMETFPMTRTFGAFWLHGGKIVGAFLEVG 147
            R   +SW+FYG   G+  + G +   +   FGAFW+H G++VGAFLE G
Sbjct:  349 RVFTLSWQFYGDNVGDSVLFGDQ---IVGKFGAFWVHNGRLVGAFLEGG 394          
BLAST of mRNA_Ecto-sp13_S_contig92814.20874.1 vs. uniprot
Match: A0A0A9NRD2_ARUDO (Pyr_redox_2 domain-containing protein n=2 Tax=Arundo donax TaxID=35708 RepID=A0A0A9NRD2_ARUDO)

HSP 1 Score: 48.9 bits (115), Expect = 1.980e-5
Identity = 24/49 (48.98%), Postives = 30/49 (61.22%), Query Frame = 1
Query:    1 RFLDISWKFYGFARGEVCVLGMETFPMTRTFGAFWLHGGKIVGAFLEVG 147
            R   +SW+FYG   GEV   G  T   +  FGA+W+H G+I GAFLE G
Sbjct:   97 RVFTLSWQFYGDNVGEVVHFGDFT-SSSPCFGAYWVHKGRITGAFLEGG 144          
BLAST of mRNA_Ecto-sp13_S_contig92814.20874.1 vs. uniprot
Match: UPI000C1D1D8D (monodehydroascorbate reductase 4, peroxisomal-like n=1 Tax=Olea europaea var. sylvestris TaxID=158386 RepID=UPI000C1D1D8D)

HSP 1 Score: 48.9 bits (115), Expect = 2.390e-5
Identity = 23/49 (46.94%), Postives = 31/49 (63.27%), Query Frame = 1
Query:    1 RFLDISWKFYGFARGEVCVLGMETFPMTRTFGAFWLHGGKIVGAFLEVG 147
            R   +SW+FYG   GEV   G  +    +TFGA+W+H G +VG+FLE G
Sbjct:  152 RVFTLSWQFYGDNIGEVFHFGDFS---GKTFGAYWIHNGHLVGSFLEGG 197          
The following BLAST results are available for this feature:
BLAST of mRNA_Ecto-sp13_S_contig92814.20874.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 12
Match NameE-valueIdentityDescription
A0A2P6TYS0_CHLSO1.210e-751.02Monodehydroascorbate reductase n=1 Tax=Chlorella s... [more]
A0A2P5B703_PARAD5.820e-747.06FAD/NAD(P)-binding domain containing protein n=2 T... [more]
A0A0A9DAU7_ARUDO1.840e-648.98Pyr_redox_2 domain-containing protein n=1 Tax=Arun... [more]
UPI000901E7CA2.780e-644.00monodehydroascorbate reductase-like n=2 Tax=Ipomoe... [more]
A0A8K0HIV5_9ROSA3.820e-650.00Uncharacterized protein n=1 Tax=Rhamnella rubriner... [more]
A0A6P6FMN7_ZIZJJ1.340e-546.43monodehydroascorbate reductase, seedling isozyme-l... [more]
MDAR1_ARATH1.830e-547.06Monodehydroascorbate reductase 1, peroxisomal n=32... [more]
A9TN13_PHYPA1.830e-544.90Predicted protein n=1 Tax=Physcomitrium patens Tax... [more]
A0A0A9NRD2_ARUDO1.980e-548.98Pyr_redox_2 domain-containing protein n=2 Tax=Arun... [more]
UPI000C1D1D8D2.390e-546.94monodehydroascorbate reductase 4, peroxisomal-like... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
Ecto-sp13_S_contig92814contigEcto-sp13_S_contig92814:105..251 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Ectocarpus species13 EcNAP12_S_4_19m OGS1.02022-07-08
Properties
Property NameValue
Taxonomic scopeEukaryota
Stop0
Start0
Seed ortholog score70.1
Seed ortholog evalue3.3e-10
Seed eggNOG ortholog2880.D7FZW4
Preferred nameMDAR1
Model size147
KEGG koko:K08232
KEGG ReactionR00095
KEGG Pathwayko00053,ko01100,map00053,map01100
Hectar predicted targeting categoryno signal peptide or anchor
GOsGO:0000166,GO:0001101,GO:0003674,GO:0003824,GO:0005488,GO:0005524,GO:0005575,GO:0005622,GO:0005623,GO:0005737,GO:0005739,GO:0005777,GO:0005778,GO:0005829,GO:0006950,GO:0006970,GO:0008144,GO:0008150,GO:0008152,GO:0009056,GO:0009266,GO:0009409,GO:0009414,GO:0009415,GO:0009507,GO:0009526,GO:0009532,GO:0009536,GO:0009570,GO:0009605,GO:0009607,GO:0009608,GO:0009610,GO:0009620,GO:0009628,GO:0009636,GO:0009651,GO:0009719,GO:0009725,GO:0009753,GO:0009941,GO:0009987,GO:0010033,GO:0010035,GO:0010038,GO:0010043,GO:0010319,GO:0016020,GO:0016491,GO:0016651,GO:0016655,GO:0016656,GO:0016999,GO:0017001,GO:0017076,GO:0017144,GO:0030554,GO:0031090,GO:0031903,GO:0031967,GO:0031975,GO:0032553,GO:0032555,GO:0032559,GO:0035639,GO:0036094,GO:0042221,GO:0042579,GO:0042737,GO:0042743,GO:0042744,GO:0043167,GO:0043168,GO:0043207,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0043900,GO:0043903,GO:0044237,GO:0044248,GO:0044422,GO:0044424,GO:0044434,GO:0044435,GO:0044438,GO:0044439,GO:0044444,GO:0044446,GO:0044464,GO:0046686,GO:0050789,GO:0050896,GO:0051186,GO:0051187,GO:0051704,GO:0051707,GO:0051716,GO:0055114,GO:0065007,GO:0070887,GO:0072593,GO:0097159,GO:0097237,GO:0097367,GO:0098588,GO:0098754,GO:0098805,GO:0098869,GO:1901265,GO:1901363,GO:1901700,GO:1990748
Exons1
EggNOG free text desc.monodehydroascorbate reductase (NADH) activity
EggNOG OGsCOG0446@1,KOG1336@2759
EC1.6.5.4
Cds size147
COG Functional cat.J
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko01000
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1681464198.5073972-CDS-Ecto-sp13_S_contig92814:104..2511681464198.5073972-CDS-Ecto-sp13_S_contig92814:104..251Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig92814 105..251 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_Ecto-sp13_S_contig92814.20874.1prot_Ecto-sp13_S_contig92814.20874.1Ectocarpus species13 EcNAP12_S_4_19mpolypeptideEcto-sp13_S_contig92814 105..251 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_Ecto-sp13_S_contig92814.20874.1

>prot_Ecto-sp13_S_contig92814.20874.1 ID=prot_Ecto-sp13_S_contig92814.20874.1|Name=mRNA_Ecto-sp13_S_contig92814.20874.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=polypeptide|length=49bp
RFLDISWKFYGFARGEVCVLGMETFPMTRTFGAFWLHGGKIVGAFLEVG
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mRNA from alignment at Ecto-sp13_S_contig92814:105..251+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_Ecto-sp13_S_contig92814.20874.1 ID=mRNA_Ecto-sp13_S_contig92814.20874.1|Name=mRNA_Ecto-sp13_S_contig92814.20874.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=mRNA|length=147bp|location=Sequence derived from alignment at Ecto-sp13_S_contig92814:105..251+ (Ectocarpus species13 EcNAP12_S_4_19m)
AGGTTCTTGGACATATCATGGAAATTTTACGGTTTCGCGAGGGGAGAAGT GTGCGTGCTCGGCATGGAAACCTTTCCGATGACAAGGACATTCGGAGCTT TCTGGCTGCATGGCGGGAAGATTGTTGGTGCTTTTCTTGAGGTGGGG
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Coding sequence (CDS) from alignment at Ecto-sp13_S_contig92814:105..251+

>mRNA_Ecto-sp13_S_contig92814.20874.1 ID=mRNA_Ecto-sp13_S_contig92814.20874.1|Name=mRNA_Ecto-sp13_S_contig92814.20874.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=CDS|length=147bp|location=Sequence derived from alignment at Ecto-sp13_S_contig92814:105..251+ (Ectocarpus species13 EcNAP12_S_4_19m)
AGGTTCTTGGACATATCATGGAAATTTTACGGTTTCGCGAGGGGAGAAGT
GTGCGTGCTCGGCATGGAAACCTTTCCGATGACAAGGACATTCGGAGCTT
TCTGGCTGCATGGCGGGAAGATTGTTGGTGCTTTTCTTGAGGTGGGG
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