mRNA_Ecto-sp13_S_contig12463.2042.1 (mRNA) Ectocarpus species13 EcNAP12_S_4_19m

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_Ecto-sp13_S_contig12463.2042.1
Unique NamemRNA_Ecto-sp13_S_contig12463.2042.1
TypemRNA
OrganismEctocarpus species13 EcNAP12_S_4_19m (Ectocarpus species13 EcNAP12_S_4_19m)
Homology
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BLAST of mRNA_Ecto-sp13_S_contig12463.2042.1 vs. uniprot
Match: D7G4D0_ECTSI (RNA polymerase II core subunit n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7G4D0_ECTSI)

HSP 1 Score: 211 bits (537), Expect = 2.440e-68
Identity = 106/107 (99.07%), Postives = 107/107 (100.00%), Query Frame = 1 
Query:    1 RRLAYTPDTKIPHAGTFEIIKEDHTLGNLLRMQLLADKQVRFAGYMQPHPLENKIHIKVQTNGDNTPVDALSAATEDLSQEMEGLREVFQEQVAAAKHAAEQEQFGS 321
            RRLAYTPDTKIPHAGTFEIIKEDHTLGNLLRMQLLAD+QVRFAGYMQPHPLENKIHIKVQTNGDNTPVDALSAATEDLSQEMEGLREVFQEQVAAAKHAAEQEQFGS
Sbjct:   64 RRLAYTPDTKIPHAGTFEIIKEDHTLGNLLRMQLLADRQVRFAGYMQPHPLENKIHIKVQTNGDNTPVDALSAATEDLSQEMEGLREVFQEQVAAAKHAAEQEQFGS 170
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BLAST of mRNA_Ecto-sp13_S_contig12463.2042.1 vs. uniprot
Match: A0A6H5JST3_9PHAE (RNA_pol_L_2 domain-containing protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JST3_9PHAE)

HSP 1 Score: 209 bits (531), Expect = 4.290e-68
Identity = 104/107 (97.20%), Postives = 107/107 (100.00%), Query Frame = 1 
Query:    1 RRLAYTPDTKIPHAGTFEIIKEDHTLGNLLRMQLLADKQVRFAGYMQPHPLENKIHIKVQTNGDNTPVDALSAATEDLSQEMEGLREVFQEQVAAAKHAAEQEQFGS 321
            ++LAYTPDTKIPHAGTFEIIKEDHTLGNLLRMQLLAD+QVRFAGYMQPHPLENKIHIKVQTNGDNTPVDALSAATEDLSQEMEGLREVFQEQVAAAKHAAEQEQFGS
Sbjct:   17 KKLAYTPDTKIPHAGTFEIIKEDHTLGNLLRMQLLADRQVRFAGYMQPHPLENKIHIKVQTNGDNTPVDALSAATEDLSQEMEGLREVFQEQVAAAKHAAEQEQFGS 123
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BLAST of mRNA_Ecto-sp13_S_contig12463.2042.1 vs. uniprot
Match: A0A836CIX8_9STRA (Putative DNA-directed RNA polymerase II 13.6 kDa polypeptide n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A836CIX8_9STRA)

HSP 1 Score: 130 bits (326), Expect = 7.150e-37
Identity = 64/104 (61.54%), Postives = 79/104 (75.96%), Query Frame = 1 
Query:    4 RLAYTPDTKIPHAGTFEIIKEDHTLGNLLRMQLLADKQVRFAGYMQPHPLENKIHIKVQTNGDNTPVDALSAATEDLSQEMEGLREVFQEQVAAAKHAAEQEQF 315
            ++ Y PDTKIP+AG F I KEDHTLGNL+RMQLL D+QVRFAGY+ PHPLEN + +KVQTNG+ TP+DALS A EDL+ E++ L + F E V A K   E  Q+
Sbjct:   18 KVDYEPDTKIPNAGNFIIKKEDHTLGNLIRMQLLQDRQVRFAGYLMPHPLENIMRVKVQTNGEVTPMDALSTAIEDLTSEVDNLTQRFAEGVLACKRLQESRQY 121
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BLAST of mRNA_Ecto-sp13_S_contig12463.2042.1 vs. uniprot
Match: K0SS38_THAOC (RNA_pol_L_2 domain-containing protein n=1 Tax=Thalassiosira oceanica TaxID=159749 RepID=K0SS38_THAOC)

HSP 1 Score: 108 bits (271), Expect = 1.680e-28
Identity = 53/85 (62.35%), Postives = 66/85 (77.65%), Query Frame = 1 
Query:    4 RLAYTPDTKIPHAGTFEIIKEDHTLGNLLRMQLLADKQVRFAGYMQPHPLENKIHIKVQTNGDNT-PVDALSAATEDLSQEMEGL 255
            ++ Y PDTK+ +AGTF + KEDHT+GNLLRMQLL D QV+FAGYM PHPL N+IH+KVQT+     P ++LSAA EDLS E + L
Sbjct:   19 KIEYQPDTKVANAGTFTLNKEDHTVGNLLRMQLLRDPQVKFAGYMHPHPLVNRIHLKVQTSTSQVAPTESLSAAIEDLSNETDHL 103
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BLAST of mRNA_Ecto-sp13_S_contig12463.2042.1 vs. uniprot
Match: A0A6U1NZH5_9STRA (Hypothetical protein n=1 Tax=Fibrocapsa japonica TaxID=94617 RepID=A0A6U1NZH5_9STRA)

HSP 1 Score: 105 bits (261), Expect = 6.040e-27
Identity = 51/94 (54.26%), Postives = 67/94 (71.28%), Query Frame = 1 
Query:    1 RRLAYTPDTKIPHAGTFEIIKEDHTLGNLLRMQLLADKQVRFAGYMQPHPLENKIHIKVQTNGDNT-PVDALSAATEDLSQEMEGLREVFQEQV 279
            ++L Y PDTKIP+AGTF + +EDHTLGNLLRMQLL D  VRFAGY  PHPL + + +K+QT      P+ A+S + EDLS E + L + F+E +
Sbjct:   17 KKLTYEPDTKIPNAGTFTLNREDHTLGNLLRMQLLQDSSVRFAGYQHPHPLVHHLLVKIQTTDPPANPIQAMSTSIEDLSSEFDFLEQRFKEDI 110
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BLAST of mRNA_Ecto-sp13_S_contig12463.2042.1 vs. uniprot
Match: A0A7S4A5Q2_9STRA (Hypothetical protein n=1 Tax=Pelagomonas calceolata TaxID=35677 RepID=A0A7S4A5Q2_9STRA)

HSP 1 Score: 103 bits (257), Expect = 2.240e-26
Identity = 51/100 (51.00%), Postives = 66/100 (66.00%), Query Frame = 1 
Query:    4 RLAYTPDTKIPHAGTFEIIKEDHTLGNLLRMQLLADKQVRFAGYMQPHPLENKIHIKVQTNGDNTPVDALSAATEDLSQEMEGLREVFQEQVAAAKHAAE 303
            R+ Y PD ++  AGTF I KEDHT+GNLLRMQLL D   RFAGY  PHPLE+  H+KV+T     PV+ +  A  DL QE+E L   F+++ A A+  A+
Sbjct:   18 RMTYEPDQRVASAGTFTINKEDHTVGNLLRMQLLRDGDTRFAGYQLPHPLEHVCHVKVETAPGRAPVEVMGGAVTDLRQEVELLDRGFRDECARAREEAD 117
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BLAST of mRNA_Ecto-sp13_S_contig12463.2042.1 vs. uniprot
Match: A0A7R9WNF7_9STRA (Hypothetical protein n=1 Tax=Craspedostauros australis TaxID=1486917 RepID=A0A7R9WNF7_9STRA)

HSP 1 Score: 103 bits (257), Expect = 2.580e-26
Identity = 51/93 (54.84%), Postives = 67/93 (72.04%), Query Frame = 1 
Query:    4 RLAYTPDTKIPHAGTFEIIKEDHTLGNLLRMQLLADKQVRFAGYMQPHPLENKIHIKVQTNGDNT-PVDALSAATEDLSQEMEGLREVFQEQV 279
            ++ YT DTK+ +AGTF++ KEDHT+GNL RMQLL D  VRFAGY  PHPL + +++K+QTN     PV+ LS+A EDLS E + L   FQE +
Sbjct:   20 KIEYTADTKVTNAGTFKLNKEDHTVGNLFRMQLLRDPSVRFAGYYHPHPLVHFLNMKIQTNNSTVAPVEVLSSAIEDLSNETDHLTSQFQEAI 112
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BLAST of mRNA_Ecto-sp13_S_contig12463.2042.1 vs. uniprot
Match: RPB11_DICDI (DNA-directed RNA polymerase II subunit rpb11 n=1 Tax=Dictyostelium discoideum TaxID=44689 RepID=RPB11_DICDI)

HSP 1 Score: 103 bits (256), Expect = 2.920e-26
Identity = 47/102 (46.08%), Postives = 71/102 (69.61%), Query Frame = 1 
Query:    1 RRLAYTPDTKIPHAGTFEIIKEDHTLGNLLRMQLLADKQVRFAGYMQPHPLENKIHIKVQTNGDNTPVDALSAATEDLSQEMEGLREVFQEQVAAAKHAAEQ 306
            +++    DTKIP+A  F I+KEDHT+GNL+RMQL+AD+ + FAGY  PHPLE+ ++I++QTN +  P++ +  + E LS+E   L   F EQV   ++ A+Q
Sbjct:   17 KKVTMVRDTKIPNASMFTILKEDHTIGNLIRMQLIADQDIIFAGYRMPHPLEHNVNIRIQTNNNTNPLECVQKSMECLSREFTSLENSFIEQVQKKRNVADQ 118
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BLAST of mRNA_Ecto-sp13_S_contig12463.2042.1 vs. uniprot
Match: F1A1H8_DICPU (RNA_pol_L_2 domain-containing protein n=1 Tax=Dictyostelium purpureum TaxID=5786 RepID=F1A1H8_DICPU)

HSP 1 Score: 103 bits (256), Expect = 2.920e-26
Identity = 47/101 (46.53%), Postives = 71/101 (70.30%), Query Frame = 1 
Query:    1 RRLAYTPDTKIPHAGTFEIIKEDHTLGNLLRMQLLADKQVRFAGYMQPHPLENKIHIKVQTNGDNTPVDALSAATEDLSQEMEGLREVFQEQVAAAKHAAE 303
            +++  T DTKIP+A  F I+KEDHT+GNL+RMQL+AD  + FAGY  PHPLE+ ++I++QTN + TP+D++  + E L+ E   + + F+  V   KH A+
Sbjct:   17 KKVTMTRDTKIPNASMFVILKEDHTVGNLVRMQLVADPDIIFAGYRMPHPLEHNVNIRIQTNQNTTPLDSIKKSIECLTNEFLNIEDQFKTMVQRKKHMAD 117
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BLAST of mRNA_Ecto-sp13_S_contig12463.2042.1 vs. uniprot
Match: F0Y9X2_AURAN (RNA_pol_L_2 domain-containing protein n=1 Tax=Aureococcus anophagefferens TaxID=44056 RepID=F0Y9X2_AURAN)

HSP 1 Score: 102 bits (255), Expect = 3.910e-26
Identity = 53/98 (54.08%), Postives = 65/98 (66.33%), Query Frame = 1 
Query:    1 RRLAYTPDTKIPHAGTFEIIKEDHTLGNLLRMQLLADKQVRFAGYMQPHPLENKIHIKVQTNGDN-TPVDALSAATEDLSQEMEGLREVFQEQVAAAK 291
            +R+ Y  D KIP+AGTF I KEDHT+GNLLRMQ+L     +FAGY  PHPL N+  +K+QT   N TP+ A   A EDL  EM+ L   F+EQVA  K
Sbjct:   17 KRMTYKNDEKIPNAGTFTINKEDHTVGNLLRMQILRSPDTKFAGYQLPHPLMNECRVKIQTTSANLTPIGAFQRAIEDLQSEMDILDRNFREQVAKKK 114
The following BLAST results are available for this feature:
BLAST of mRNA_Ecto-sp13_S_contig12463.2042.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
D7G4D0_ECTSI2.440e-6899.07RNA polymerase II core subunit n=1 Tax=Ectocarpus ... [more]
A0A6H5JST3_9PHAE4.290e-6897.20RNA_pol_L_2 domain-containing protein n=1 Tax=Ecto... [more]
A0A836CIX8_9STRA7.150e-3761.54Putative DNA-directed RNA polymerase II 13.6 kDa p... [more]
K0SS38_THAOC1.680e-2862.35RNA_pol_L_2 domain-containing protein n=1 Tax=Thal... [more]
A0A6U1NZH5_9STRA6.040e-2754.26Hypothetical protein n=1 Tax=Fibrocapsa japonica T... [more]
A0A7S4A5Q2_9STRA2.240e-2651.00Hypothetical protein n=1 Tax=Pelagomonas calceolat... [more]
A0A7R9WNF7_9STRA2.580e-2654.84Hypothetical protein n=1 Tax=Craspedostauros austr... [more]
RPB11_DICDI2.920e-2646.08DNA-directed RNA polymerase II subunit rpb11 n=1 T... [more]
F1A1H8_DICPU2.920e-2646.53RNA_pol_L_2 domain-containing protein n=1 Tax=Dict... [more]
F0Y9X2_AURAN3.910e-2654.08RNA_pol_L_2 domain-containing protein n=1 Tax=Aure... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
Ecto-sp13_S_contig12463contigEcto-sp13_S_contig12463:3020..4444 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Ectocarpus species13 EcNAP12_S_4_19m OGS1.02022-07-08
Properties
Property NameValue
Taxonomic scopeEukaryota
Stop1
Start0
Seed ortholog score212.2
Seed ortholog evalue1.2e-52
Seed eggNOG ortholog2880.D7G4D0
Preferred nameRPB11
Model size324
KEGG rclassRC02795
KEGG koko:K03008
KEGG ReactionR00435,R00441,R00442,R00443
KEGG Pathwayko00230,ko00240,ko01100,ko03020,ko05016,ko05169,map00230,map00240,map01100,map03020,map05016,map05169
KEGG ModuleM00180
Hectar predicted targeting categoryother localisation
GOsGO:0000428,GO:0005575,GO:0005622,GO:0005623,GO:0005634,GO:0005654,GO:0005665,GO:0006139,GO:0006351,GO:0006353,GO:0006366,GO:0006369,GO:0006725,GO:0006807,GO:0008150,GO:0008152,GO:0009058,GO:0009059,GO:0009987,GO:0010467,GO:0016070,GO:0016591,GO:0018130,GO:0019438,GO:0030880,GO:0031974,GO:0031981,GO:0032774,GO:0032991,GO:0034641,GO:0034645,GO:0034654,GO:0043170,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0043233,GO:0044237,GO:0044238,GO:0044249,GO:0044260,GO:0044271,GO:0044422,GO:0044424,GO:0044428,GO:0044446,GO:0044451,GO:0044464,GO:0046483,GO:0055029,GO:0061695,GO:0070013,GO:0071704,GO:0090304,GO:0097659,GO:1901360,GO:1901362,GO:1901576,GO:1902494,GO:1990234
Exons3
EggNOG free text desc.RNA polymerase II activity
EggNOG OGsCOG1761@1,KOG4392@2759
Ec32 ortholog descriptionDNA-directed RNA polymerase, RBP11-like dimerisation domain
Ec32 orthologEc-00_007330.1
Cds size324
COG Functional cat.K
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEbr01611,ko00000,ko00001,ko00002,ko03021,ko03400
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1681462663.7079213-CDS-Ecto-sp13_S_contig12463:3019..31691681462663.7079213-CDS-Ecto-sp13_S_contig12463:3019..3169Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig12463 3020..3169 -
1681462663.7187216-CDS-Ecto-sp13_S_contig12463:3648..37271681462663.7187216-CDS-Ecto-sp13_S_contig12463:3648..3727Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig12463 3649..3727 -
1681462663.7269685-CDS-Ecto-sp13_S_contig12463:4349..44441681462663.7269685-CDS-Ecto-sp13_S_contig12463:4349..4444Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig12463 4350..4444 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_Ecto-sp13_S_contig12463.2042.1prot_Ecto-sp13_S_contig12463.2042.1Ectocarpus species13 EcNAP12_S_4_19mpolypeptideEcto-sp13_S_contig12463 3020..4444 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_Ecto-sp13_S_contig12463.2042.1

>prot_Ecto-sp13_S_contig12463.2042.1 ID=prot_Ecto-sp13_S_contig12463.2042.1|Name=mRNA_Ecto-sp13_S_contig12463.2042.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=polypeptide|length=108bp
RRLAYTPDTKIPHAGTFEIIKEDHTLGNLLRMQLLADKQVRFAGYMQPHP
LENKIHIKVQTNGDNTPVDALSAATEDLSQEMEGLREVFQEQVAAAKHAA
EQEQFGS*
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mRNA from alignment at Ecto-sp13_S_contig12463:3020..4444-

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_Ecto-sp13_S_contig12463.2042.1 ID=mRNA_Ecto-sp13_S_contig12463.2042.1|Name=mRNA_Ecto-sp13_S_contig12463.2042.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=mRNA|length=1425bp|location=Sequence derived from alignment at Ecto-sp13_S_contig12463:3020..4444- (Ectocarpus species13 EcNAP12_S_4_19m)
CGCAGGCTTGCCTACACCCCAGACACCAAAATCCCGCACGCCGGGACCTT
CGAGATCATCAAAGAGGACCACACGCTCGGAAACCTGCTGCGAATGTGAG
CTGAGAAGTGCGATTGAGAGGATGGGTTGTTGTGCATGCCCGAAGGACGA
GGAAGTCTCTTAAGGTCCACGCGTTTGTGTTGCTACAGCTGCTGCTGTGG
TGGACACAAACCCCAAGCTTTGCCATTGGTATGTGTGAGGGACTGTGATT
GCCTTGGTCTATCGACCATTTCACCACGTCCCTCTCATTCGAGAACGTAC
TGGTGTGTTGAGTAGTCATTCGCGTTTAGCAAGTTGGGAACGGCTGAGGC
CATTTTCACCAGCGGCCTGCGTGAGAGCTTGGGAAACGGTAGGCCACGCG
TAACTACAGTGACAGATGCGGCGTGTGCGGTACAATGATACCCACAACGG
TGGTGATAGGTTGACATAATTGATTTGTTGTAGTGCGTCACAATCACAGC
GCGACGCCTTTGTACGACGGCGGTTCCTCCGCACCACCTTGTTTTTCCTC
GCTGATGCGTATACACTGCTTTTTGAGGCGGACAGGTAGGAAATCAATTC
AACTGTTCCGGTGTACAATTGGTGGGGGCGTTGTCGAGGGCTTAACTTCT
TTTGTGTCCTCTTCGTCTCTGCCCCGTTCGGTCCCGTTGCTTTTCCTCTG
TCCGTCGTGTACGACAGGCAACTGCTAGCCGACAAGCAGGTTCGGTTTGC
CGGGTACATGCAACCTCATCCGTTGGAGAACAAGATCCATATCAAGGTGC
CCTTTGTCACAAACTTTTCGCGTCCCAGTCCGGCGCTGTACGCTGCCATG
TAGGACTTATCAGACCCATCGACCCCGCTGAAAAATCAGGGATGGCGGGG
CAACGGACTTGGAGTTGAGGAACGCCACCGCTGTTGATAAATGTCAGGCG
GAGATGCAGCAAACGTGCTAGGTTCCCATGAGTGCTTCGGATCGGAGAAC
AATGTCTAGCATTCACCACGAGCAAGCGATGGCACGTATGTGTTGCGGGT
CGCTCAACGAAAAGCTGTATTTACTGCAGTCCATCTCCAACAAACGGACT
TTGATACCATACGATTTACCTCGATATGTACTTCAGCAAGGGCATCCACG
TCTTTTTTCACACGCCACACACATTTCTAGGTCGTACGATCAAACGAACG
CGCTCGAAAAGCTCACTTGCAACTGATGTACCCAAGCCTTTGAAAACCAC
CTCAAACACAAAACATGTTTGTCAGGTCCAAACGAACGGTGATAACACAC
CGGTGGATGCTTTATCGGCGGCGACTGAGGATCTGTCACAGGAGATGGAA
GGGCTGCGGGAAGTTTTCCAAGAGCAGGTTGCGGCTGCCAAGCACGCCGC
GGAGCAGGAGCAGTTCGGGTCATAG
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Coding sequence (CDS) from alignment at Ecto-sp13_S_contig12463:3020..4444-

>mRNA_Ecto-sp13_S_contig12463.2042.1 ID=mRNA_Ecto-sp13_S_contig12463.2042.1|Name=mRNA_Ecto-sp13_S_contig12463.2042.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=CDS|length=324bp|location=Sequence derived from alignment at Ecto-sp13_S_contig12463:3020..4444- (Ectocarpus species13 EcNAP12_S_4_19m)
CGCAGGCTTGCCTACACCCCAGACACCAAAATCCCGCACGCCGGGACCTT
CGAGATCATCAAAGAGGACCACACGCTCGGAAACCTGCTGCGAATGCAAC
TGCTAGCCGACAAGCAGGTTCGGTTTGCCGGGTACATGCAACCTCATCCG
TTGGAGAACAAGATCCATATCAAGGTCCAAACGAACGGTGATAACACACC
GGTGGATGCTTTATCGGCGGCGACTGAGGATCTGTCACAGGAGATGGAAG
GGCTGCGGGAAGTTTTCCAAGAGCAGGTTGCGGCTGCCAAGCACGCCGCG
GAGCAGGAGCAGTTCGGGTCATAG
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