mRNA_Ecto-sp13_S_contig8733.20199.1 (mRNA) Ectocarpus species13 EcNAP12_S_4_19m

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_Ecto-sp13_S_contig8733.20199.1
Unique NamemRNA_Ecto-sp13_S_contig8733.20199.1
TypemRNA
OrganismEctocarpus species13 EcNAP12_S_4_19m (Ectocarpus species13 EcNAP12_S_4_19m)
Homology
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BLAST of mRNA_Ecto-sp13_S_contig8733.20199.1 vs. uniprot
Match: D7FI62_ECTSI (Protein tyrosine kinase n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FI62_ECTSI)

HSP 1 Score: 734 bits (1895), Expect = 9.870e-253
Identity = 423/490 (86.33%), Postives = 431/490 (87.96%), Query Frame = 1 
Query:    1 MMSSTATPRSTGTNTAGPRLAPGPVPAAPPTATTPARFYHSKEVPRLSKALSVVANLLKSPAKVPSRLFDVSKAIVLLALTQRFSPTSIKVRQLEQSSGADRSRGAVNTISTWARQVSKQRANKDQAVLELLGNAARAAPATKQTLLEHFGMGGTNPASVCKELSILLTALVKELRGEAGRDSPPSFSSSATNGDKGGTATGAAAPSADSATTVTVIATDDVVPLRRLLASTDSAVTGADTTAQASLEGVNPEPAIAIAATXXXXESKREVPPPSATAVAAEIDATAGDVVGPGEPSASLHKEENIDSAAATPGMVVPASLGTGTPAPGVKSVFAAPADMAASCPGGPSRSRKCSALKVGKGEGGWGGGRGGGHLVKEGHAVGTGDGRSSTVRQGSNRQKPVVANVGRPSAQASANTAARARSRGLAFIARPAGTPSSSGAGERGVPRDGQLKAAAANRALGSVRGGGPRKQGCELSRSDRDGAVAAATA 1470
            MMSSTATPRS GT  AGPRLAPG VPAAPPTATTP RFYH+ EVPRLSKALSVVANLLKSPAKV SRLFDV KAIVLLALTQRFSPTSIKVRQLEQSSGADRSRGAVNTISTWARQVSKQRANKDQAVLELL NAARAAPATKQTLLEHFGMGGTNPASVCKELSILLTAL+KEL GEAG DSP  FSSSAT+GDKGG AT AA       TTV  IATDDVVP RRL ASTDSAVTGADT  QASLEGVN + AIAIAAT    ESKR+   P ATA AAE+ ATAGDVVGPG+PSAS H EENI SAAATPGMVVPASL TGTPAPGVKSV AAPADMAA C GGPS SRKCSALKVGKGEGGWGGGRGGGHLVKEGH VGT DGRSSTVRQGSNRQKP V N GRPSA ASAN AARARSRGLAFIARPAGTP SSGAGERGVPRD Q+KAAAANRA G VRGGGPRKQGCELSRSDRDGAVAAATA
Sbjct:    1 MMSSTATPRSAGTP-AGPRLAPGSVPAAPPTATTPTRFYHNNEVPRLSKALSVVANLLKSPAKVSSRLFDVGKAIVLLALTQRFSPTSIKVRQLEQSSGADRSRGAVNTISTWARQVSKQRANKDQAVLELLDNAARAAPATKQTLLEHFGMGGTNPASVCKELSILLTALIKELHGEAGSDSPSPFSSSATSGDKGGMATAAAXXXXXXXTTVAAIATDDVVPRRRLPASTDSAVTGADTAVQASLEGVNAKSAIAIAATATADESKRQFRAPPATATAAEVGATAGDVVGPGKPSASSHNEENIGSAAATPGMVVPASLETGTPAPGVKSVSAAPADMAALCRGGPSSSRKCSALKVGKGEGGWGGGRGGGHLVKEGHVVGTRDGRSSTVRQGSNRQKPTVVNAGRPSAPASANAAARARSRGLAFIARPAGTPFSSGAGERGVPRDRQVKAAAANRAPGGVRGGGPRKQGCELSRSDRDGAVAAATA 489
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BLAST of mRNA_Ecto-sp13_S_contig8733.20199.1 vs. uniprot
Match: A0A6H5KSE9_9PHAE (Protein kinase domain-containing protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5KSE9_9PHAE)

HSP 1 Score: 635 bits (1638), Expect = 2.050e-212
Identity = 363/429 (84.62%), Postives = 377/429 (87.88%), Query Frame = 1 
Query:  181 PAKVPSRLFDVSKAIVLLALTQRFSPTSIKVRQLEQSSGADRSRGAVNTISTWARQVSKQRANKDQAVLELLGNAARAAPATKQTLLEHFGMGGTNPASVCKELSILLTALVKELRGEAGRDSPPSFSSSATNGDKGGTATGAAAPSADSATTVTVIATDDVVPLRRLLASTDSAVTGADTTAQASLEGVNPEPAIAIAATXXXXESKREVPPPSATAVAAEIDATAGDVVGPGEPSASLHKEENIDSAAATPGMVVPASLGTGTPAPGVKSVFAAPADMAASCPGGPSRSRKCSALKVGKGEGGWGGGRGGGHLVKEGHAVGTGDGRSSTVRQGSNRQKPVVANVGRPSAQASANTAARARSRGLAFIARPAGTPSSSGAGERGVPRDGQLKAAAANRALGSVRGGGPRKQGCELSRSDRDGAVAAAT 1467
            PAKVPSRLFDVSKAIVLLALTQRFSPTSIKV+QLEQSSGADRSRGAVNTISTWARQVSK+RANKDQ+VLELL NAARAAPATKQTLLEHFGMGGTNPASVCKELSILLTAL+KELRGEAGRDSP  F+SSAT+GDKGG AT  AA           IA DDV P RRL ASTDSAVTG  T  +ASLEGVN +PAIAI AT    ESKREVP PSATAVAAE+DATAG++VGPG+PSAS HKEENIDSAAATPGMV PASL TGTPAPGVKSV AAPADMAASCPGGPS SRK SALKVGKGEG WG GRGGGHLVKEGH VGT DGRSS VRQGS RQKPVVAN GRPSAQASAN AARARSRGLAFIA PAGTP SSGAGERGVPRDGQ++AAAANRA G  RGGGPRKQ CELSRSDRDGAVAAAT
Sbjct:    8 PAKVPSRLFDVSKAIVLLALTQRFSPTSIKVQQLEQSSGADRSRGAVNTISTWARQVSKKRANKDQSVLELLDNAARAAPATKQTLLEHFGMGGTNPASVCKELSILLTALIKELRGEAGRDSPSPFTSSATSGDKGGMATTGAA-----------IAPDDVAPRRRLPASTDSAVTGVATAVKASLEGVNAKPAIAIVATATADESKREVPAPSATAVAAEVDATAGNMVGPGKPSASSHKEENIDSAAATPGMVRPASLETGTPAPGVKSVSAAPADMAASCPGGPSSSRKFSALKVGKGEGDWGDGRGGGHLVKEGHVVGTRDGRSSKVRQGSTRQKPVVANAGRPSAQASANAAARARSRGLAFIAHPAGTPFSSGAGERGVPRDGQVEAAAANRAQGDARGGGPRKQACELSRSDRDGAVAAAT 425
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BLAST of mRNA_Ecto-sp13_S_contig8733.20199.1 vs. uniprot
Match: D7FI46_ECTSI (p38 Map Kinase family member (Pmk-1) n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FI46_ECTSI)

HSP 1 Score: 633 bits (1632), Expect = 2.930e-212
Identity = 371/488 (76.02%), Postives = 397/488 (81.35%), Query Frame = 1 
Query:    1 MMSSTATPRSTGTNTAGPRLAPGPVPAAPPTATTPARFYHSKEVPRLSKALSVVANLLKSPAKVPSRLFDVSKAIVLLALTQRFSPTSIKVRQLEQSSGADRSRGAVNTISTWARQVSKQRANKDQAVLELLGNAARAAPATKQTLLEHFGMGGTNPASVCKELSILLTALVKELRGEAGRDSPPSFSSSATNGDKGGTATGAAAPSADSATTVTVIATDDVVPLRRLLASTDSAVTGADTTAQASLEGVNPEPAIAIAATXXXXESKREVPPPSATAVAAEIDATAGDVVGPGEPSASLHKEENIDSAAATPGMVVPASLGTGTPAPGVKSVFAAPADMAASCPGGPSRS--RKCSALKVGKGEGGWGGGRGGGHLVKEGHAVGTGDGRSSTVRQGSNRQKPVVANVGRPSAQASANTAARARSRGLAFIARPAGTPSSSGAGERGVPRDGQLKAAAANRALGSVRGGGPRKQGCELSRSDRDGAVA 1458
            M S +ATPRSTGT  AGPRLAPGP+PAAPPTATT +RF+H  +V RL+KALSVVAN+LKSPAKV SRLFD+ KAIVLLALT RFSPTS+KVRQLEQSS ADRSRGAVNTISTWARQVS QR NKDQAVLELL NAARAAPATKQTLLE FGMGG+NPASVCKELSILLTALVKEL GEAG DSP   SSSAT+GDKGGTA   A  + DSATTV  +AT+DV P   L AST   V GADT  ++SLEG   +PAIAIA T    ESK E P   ATA AAE+ ATAGDV+ PG+ SAS HKE+NID AAATPG+VVPASLGTGTPAPGVKSV AAPAD AAS PGGPS S  R CSALKVGKGEGGWGGGRGGGHLVKEGH V T DGRSSTV QGSNRQKPVVAN GRP+AQASAN  AR RSRGLAFIARP G P  S A ERGVPRDGQ+KAAAANRA   VRGG   K+ CELSR   +G VA
Sbjct:    2 MTSDSATPRSTGTP-AGPRLAPGPIPAAPPTATTVSRFHHKNDVRRLTKALSVVANILKSPAKVSSRLFDIGKAIVLLALTHRFSPTSMKVRQLEQSSAADRSRGAVNTISTWARQVSNQRMNKDQAVLELLDNAARAAPATKQTLLERFGMGGSNPASVCKELSILLTALVKELHGEAGSDSPSLSSSSATSGDKGGTAKAVATSATDSATTVAAVATEDVTPHGGLPASTAREVAGADTGVKSSLEGAKAKPAIAIAVTTTADESKHEFPASPATATAAEVGATAGDVIEPGKASASSHKEQNIDGAAATPGVVVPASLGTGTPAPGVKSVSAAPADKAASYPGGPSSSSSRMCSALKVGKGEGGWGGGRGGGHLVKEGHVVETRDGRSSTVCQGSNRQKPVVANAGRPNAQASANAVARVRSRGLAFIARPGGAPLRSSASERGVPRDGQMKAAAANRARRGVRGGDTCKRACELSRPGHNGVVA 488
The following BLAST results are available for this feature:
BLAST of mRNA_Ecto-sp13_S_contig8733.20199.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 3
Match NameE-valueIdentityDescription
D7FI62_ECTSI9.870e-25386.33Protein tyrosine kinase n=1 Tax=Ectocarpus silicul... [more]
A0A6H5KSE9_9PHAE2.050e-21284.62Protein kinase domain-containing protein n=1 Tax=E... [more]
D7FI46_ECTSI2.930e-21276.02p38 Map Kinase family member (Pmk-1) n=1 Tax=Ectoc... [more]
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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
Ecto-sp13_S_contig8733contigEcto-sp13_S_contig8733:4684..6699 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Ectocarpus species13 EcNAP12_S_4_19m OGS1.02022-07-08
Properties
Property NameValue
Taxonomic scopeEukaryota
Stop0
Start1
Seed ortholog score742.3
Seed ortholog evalue1.5e-211
Seed eggNOG ortholog2880.D7FI62
Model size1512
KEGG koko:K02836,ko:K08818,ko:K10772
KEGG Pathwayko03410,map03410
KEGG ModuleM00296
Hectar predicted targeting categoryother localisation
Exons2
EggNOG free text desc.cyclin-dependent protein serine/threonine kinase activity
EggNOG OGsKOG0663@1,KOG0663@2759
Ec32 ortholog descriptionSerine/threonine/dual specificity protein kinase, catalytic domain
Ec32 orthologEc-10_003690.1
EC2.7.11.22,4.2.99.18
Cds size1512
COG Functional cat.T
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko00002,ko01000,ko01001,ko03012,ko03041,ko03400
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1681464145.929356-CDS-Ecto-sp13_S_contig8733:4683..48621681464145.929356-CDS-Ecto-sp13_S_contig8733:4683..4862Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig8733 4684..4862 +
1681464145.942281-CDS-Ecto-sp13_S_contig8733:5366..66991681464145.942281-CDS-Ecto-sp13_S_contig8733:5366..6699Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig8733 5367..6699 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_Ecto-sp13_S_contig8733.20199.1prot_Ecto-sp13_S_contig8733.20199.1Ectocarpus species13 EcNAP12_S_4_19mpolypeptideEcto-sp13_S_contig8733 4684..6699 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_Ecto-sp13_S_contig8733.20199.1

>prot_Ecto-sp13_S_contig8733.20199.1 ID=prot_Ecto-sp13_S_contig8733.20199.1|Name=mRNA_Ecto-sp13_S_contig8733.20199.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=polypeptide|length=504bp
MMSSTATPRSTGTNTAGPRLAPGPVPAAPPTATTPARFYHSKEVPRLSKA
LSVVANLLKSPAKVPSRLFDVSKAIVLLALTQRFSPTSIKVRQLEQSSGA
DRSRGAVNTISTWARQVSKQRANKDQAVLELLGNAARAAPATKQTLLEHF
GMGGTNPASVCKELSILLTALVKELRGEAGRDSPPSFSSSATNGDKGGTA
TGAAAPSADSATTVTVIATDDVVPLRRLLASTDSAVTGADTTAQASLEGV
NPEPAIAIAATATADESKREVPPPSATAVAAEIDATAGDVVGPGEPSASL
HKEENIDSAAATPGMVVPASLGTGTPAPGVKSVFAAPADMAASCPGGPSR
SRKCSALKVGKGEGGWGGGRGGGHLVKEGHAVGTGDGRSSTVRQGSNRQK
PVVANVGRPSAQASANTAARARSRGLAFIARPAGTPSSSGAGERGVPRDG
QLKAAAANRALGSVRGGGPRKQGCELSRSDRDGAVAAATALAAATAAAKP
AAAA
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mRNA from alignment at Ecto-sp13_S_contig8733:4684..6699+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_Ecto-sp13_S_contig8733.20199.1 ID=mRNA_Ecto-sp13_S_contig8733.20199.1|Name=mRNA_Ecto-sp13_S_contig8733.20199.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=mRNA|length=2016bp|location=Sequence derived from alignment at Ecto-sp13_S_contig8733:4684..6699+ (Ectocarpus species13 EcNAP12_S_4_19m)
ATGATGTCGTCCACTGCTACACCCCGCTCGACGGGTACTAATACGGCGGG
CCCCCGCCTCGCCCCCGGACCTGTTCCGGCAGCACCACCGACGGCAACAA
CGCCGGCCCGGTTTTATCACAGTAAGGAAGTCCCTCGCCTGTCGAAGGCC
CTTTCGGTGGTGGCGAACCTTCTTAAATCGTGAGCGCTTCAACCTTCTTG
CACTTCCCCTGCGGTCTAGACATTTCCTAACCCCGCTGTAATGTCATTCC
TCAGCCGCTTTTTTCGCACGGGCATATTCTCCGTTGTCTGCTGCAAGCGA
AAACAACGCCTAGAAACTGCCACCATCCCAACCGCGAACAACCACGTGGG
ATTTGTGTGATCATTGAGGGCCGATTCCCCTCGGTGTAGTTGTGTTCCGG
GCGGCTTCCTGGTTGGAGTTGCCCTTCGTTCGCCGGACCCAGTCCTCTCC
ATGGTGCTAACCCTCTCGACGTTGCTGGTGAGCGTTGTCTTTGGTACCCT
AGAGCATCTACAATACATTCCTGGGTTGAAGCTGAAGTTGCGCGCGTGGT
AGTGCAAACGAGGAAGTTGACTACGGGGTGGCACTCAGCAAAGTAAGTGT
TTTTCGTTCCCTTTCGGCGACACACGCGATCGTTGCCCGCCCGCGCGCAC
GTCCCGTCCTGCCCTTTCTGTTTCTCTTTTCAGGCCTGCCAAGGTGCCCA
GCCGCCTTTTCGACGTCAGCAAGGCCATTGTCCTCCTCGCCCTGACCCAG
AGGTTCTCACCCACCTCCATCAAAGTGCGGCAACTCGAACAGTCGTCTGG
TGCCGATCGCAGCCGCGGCGCCGTCAACACCATCTCAACCTGGGCGAGGC
AAGTCAGCAAACAGCGCGCGAACAAGGACCAGGCCGTGCTAGAGCTCCTC
GGCAACGCGGCGAGAGCCGCTCCCGCCACGAAGCAGACGCTGCTCGAACA
TTTCGGCATGGGAGGAACAAACCCGGCGTCCGTGTGCAAGGAGCTTAGCA
TCCTGCTCACGGCGTTGGTTAAGGAGCTGCGCGGCGAGGCGGGAAGGGAT
TCTCCTCCCTCCTTTTCTTCATCGGCGACCAATGGCGACAAAGGCGGCAC
GGCTACGGGAGCGGCCGCTCCGTCTGCCGACTCTGCCACCACTGTAACCG
TCATCGCCACAGACGACGTCGTCCCGCTGCGCAGACTGCTGGCCTCAACG
GATAGCGCAGTGACCGGGGCGGACACAACAGCCCAGGCTTCGCTCGAGGG
GGTAAATCCTGAACCTGCGATAGCGATTGCGGCCACGGCTACGGCGGACG
AAAGCAAGCGCGAGGTCCCTCCTCCGTCTGCTACCGCGGTCGCAGCCGAG
ATCGATGCGACGGCCGGCGACGTGGTCGGGCCGGGCGAACCATCAGCATC
CTTACACAAGGAAGAGAACATCGACAGTGCCGCTGCTACGCCCGGTATGG
TAGTGCCTGCTTCTCTCGGGACCGGCACTCCTGCACCTGGCGTCAAGTCA
GTTTTCGCCGCCCCCGCCGACATGGCGGCGTCGTGTCCAGGTGGTCCCAG
CAGAAGCAGGAAGTGCTCTGCTCTCAAGGTGGGCAAGGGAGAGGGTGGCT
GGGGAGGTGGGCGAGGTGGTGGACACCTCGTGAAGGAAGGCCATGCGGTG
GGAACTGGGGATGGTCGCAGCTCTACGGTTCGTCAGGGTAGCAACCGCCA
GAAACCGGTCGTCGCCAATGTCGGTCGACCAAGTGCGCAGGCAAGCGCCA
ACACTGCGGCAAGAGCACGATCGCGGGGCTTAGCCTTCATCGCTCGCCCG
GCCGGGACGCCGTCCAGCAGCGGTGCTGGTGAACGAGGAGTTCCGCGTGA
CGGTCAGCTTAAGGCGGCGGCAGCGAACCGGGCACTAGGAAGTGTAAGGG
GAGGAGGCCCTCGTAAGCAAGGCTGCGAGCTCTCGCGGTCTGATCGCGAT
GGCGCTGTCGCGGCGGCGACGGCGTTAGCGGCAGCAACAGCGGCAGCGAA
ACCCGCAGCGGCTGCA
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Coding sequence (CDS) from alignment at Ecto-sp13_S_contig8733:4684..6699+

>mRNA_Ecto-sp13_S_contig8733.20199.1 ID=mRNA_Ecto-sp13_S_contig8733.20199.1|Name=mRNA_Ecto-sp13_S_contig8733.20199.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=CDS|length=1512bp|location=Sequence derived from alignment at Ecto-sp13_S_contig8733:4684..6699+ (Ectocarpus species13 EcNAP12_S_4_19m)
ATGATGTCGTCCACTGCTACACCCCGCTCGACGGGTACTAATACGGCGGG
CCCCCGCCTCGCCCCCGGACCTGTTCCGGCAGCACCACCGACGGCAACAA
CGCCGGCCCGGTTTTATCACAGTAAGGAAGTCCCTCGCCTGTCGAAGGCC
CTTTCGGTGGTGGCGAACCTTCTTAAATCGCCTGCCAAGGTGCCCAGCCG
CCTTTTCGACGTCAGCAAGGCCATTGTCCTCCTCGCCCTGACCCAGAGGT
TCTCACCCACCTCCATCAAAGTGCGGCAACTCGAACAGTCGTCTGGTGCC
GATCGCAGCCGCGGCGCCGTCAACACCATCTCAACCTGGGCGAGGCAAGT
CAGCAAACAGCGCGCGAACAAGGACCAGGCCGTGCTAGAGCTCCTCGGCA
ACGCGGCGAGAGCCGCTCCCGCCACGAAGCAGACGCTGCTCGAACATTTC
GGCATGGGAGGAACAAACCCGGCGTCCGTGTGCAAGGAGCTTAGCATCCT
GCTCACGGCGTTGGTTAAGGAGCTGCGCGGCGAGGCGGGAAGGGATTCTC
CTCCCTCCTTTTCTTCATCGGCGACCAATGGCGACAAAGGCGGCACGGCT
ACGGGAGCGGCCGCTCCGTCTGCCGACTCTGCCACCACTGTAACCGTCAT
CGCCACAGACGACGTCGTCCCGCTGCGCAGACTGCTGGCCTCAACGGATA
GCGCAGTGACCGGGGCGGACACAACAGCCCAGGCTTCGCTCGAGGGGGTA
AATCCTGAACCTGCGATAGCGATTGCGGCCACGGCTACGGCGGACGAAAG
CAAGCGCGAGGTCCCTCCTCCGTCTGCTACCGCGGTCGCAGCCGAGATCG
ATGCGACGGCCGGCGACGTGGTCGGGCCGGGCGAACCATCAGCATCCTTA
CACAAGGAAGAGAACATCGACAGTGCCGCTGCTACGCCCGGTATGGTAGT
GCCTGCTTCTCTCGGGACCGGCACTCCTGCACCTGGCGTCAAGTCAGTTT
TCGCCGCCCCCGCCGACATGGCGGCGTCGTGTCCAGGTGGTCCCAGCAGA
AGCAGGAAGTGCTCTGCTCTCAAGGTGGGCAAGGGAGAGGGTGGCTGGGG
AGGTGGGCGAGGTGGTGGACACCTCGTGAAGGAAGGCCATGCGGTGGGAA
CTGGGGATGGTCGCAGCTCTACGGTTCGTCAGGGTAGCAACCGCCAGAAA
CCGGTCGTCGCCAATGTCGGTCGACCAAGTGCGCAGGCAAGCGCCAACAC
TGCGGCAAGAGCACGATCGCGGGGCTTAGCCTTCATCGCTCGCCCGGCCG
GGACGCCGTCCAGCAGCGGTGCTGGTGAACGAGGAGTTCCGCGTGACGGT
CAGCTTAAGGCGGCGGCAGCGAACCGGGCACTAGGAAGTGTAAGGGGAGG
AGGCCCTCGTAAGCAAGGCTGCGAGCTCTCGCGGTCTGATCGCGATGGCG
CTGTCGCGGCGGCGACGGCGTTAGCGGCAGCAACAGCGGCAGCGAAACCC
GCAGCGGCTGCA
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