mRNA_Ecto-sp13_S_contig8724.20192.1 (mRNA) Ectocarpus species13 EcNAP12_S_4_19m

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_Ecto-sp13_S_contig8724.20192.1
Unique NamemRNA_Ecto-sp13_S_contig8724.20192.1
TypemRNA
OrganismEctocarpus species13 EcNAP12_S_4_19m (Ectocarpus species13 EcNAP12_S_4_19m)
Homology
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BLAST of mRNA_Ecto-sp13_S_contig8724.20192.1 vs. uniprot
Match: D7G047_ECTSI (Topo_C_assoc domain-containing protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7G047_ECTSI)

HSP 1 Score: 121 bits (303), Expect = 3.430e-33
Identity = 104/122 (85.25%), Postives = 107/122 (87.70%), Query Frame = 1 
Query:    1 MDPRVSVAWCKRHEGPV---FAKTLQAKFV*AMNVPPDWNFSYETLYNSKQSLLDVPRPPGXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEGSACASAASPKNPEVKGKGKAKNVED 357
            MDPR+SVAWCKRHE PV   FAKTLQ KFV AMNVPPDWNFSYETLYNSKQS+LDVPRPPGXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX        ASPKNPEVKGKGKAK VE+
Sbjct:    1 MDPRMSVAWCKRHEVPVEKVFAKTLQNKFVWAMNVPPDWNFSYETLYNSKQSVLDVPRPPGXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX--XXXXXASPKNPEVKGKGKAKKVEE 120
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BLAST of mRNA_Ecto-sp13_S_contig8724.20192.1 vs. uniprot
Match: D7G042_ECTSI (TOPEUc domain-containing protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7G042_ECTSI)

HSP 1 Score: 94.7 bits (234), Expect = 3.210e-20
Identity = 60/118 (50.85%), Postives = 61/118 (51.69%), Query Frame = 1 
Query:    1 MDPRVSVAWCKRHEGPVFAKTLQAKFV*AMNVPPDWNFSYETLYNSKQSLLDVPRPPGXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEGSACASAASPKNPEVKGKGKAKNVE 354
            MDPRV            FAKTLQ KFV AMNVPPDWNFSYETLYNSKQS+LDVPRPPG                                          ASPKNPEVKGKGKAK VE
Sbjct:  934 MDPRV------------FAKTLQNKFVWAMNVPPDWNFSYETLYNSKQSVLDVPRPPG-----------------------------------------GASPKNPEVKGKGKAKKVE 998
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BLAST of mRNA_Ecto-sp13_S_contig8724.20192.1 vs. uniprot
Match: A0A6H5K298_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5K298_9PHAE)

HSP 1 Score: 74.3 bits (181), Expect = 2.290e-13
Identity = 78/100 (78.00%), Postives = 82/100 (82.00%), Query Frame = 1 
Query:   55 AKTLQAKFV*AMNVPPDWNFSYETLYNSKQSLLDVPRPPGXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEGSACASAASPKNPEVKGKGKAKNVE 354
            ++ L+ KF  AMNVPPDWNFSYETLYNSKQS+LDVPRPPGXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX             PEVKGKGKAK VE
Sbjct:   93 SRRLRDKFAWAMNVPPDWNFSYETLYNSKQSVLDVPRPPGXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXPEVKGKGKAKQVE 192
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BLAST of mRNA_Ecto-sp13_S_contig8724.20192.1 vs. uniprot
Match: A0A836C9X0_9STRA (DNA topoisomerase I n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A836C9X0_9STRA)

HSP 1 Score: 73.2 bits (178), Expect = 1.110e-12
Identity = 35/58 (60.34%), Postives = 41/58 (70.69%), Query Frame = 1 
Query:    1 MDPRVSVAWCKRHEGPV---FAKTLQAKFV*AMNVPPDWNFSYETLYNSKQSLLDVPR 165
            MDPR+SVAWCKR+E P+   FAKTL+ KFV AMNVPPDW F +ETL        D P+
Sbjct:  613 MDPRISVAWCKRNEVPIERIFAKTLRDKFVWAMNVPPDWRFDFETLRAVTAESSDEPK 670
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BLAST of mRNA_Ecto-sp13_S_contig8724.20192.1 vs. uniprot
Match: A0A482T3K1_9ARCH (Topo_C_assoc domain-containing protein n=1 Tax=archaeon TaxID=1906665 RepID=A0A482T3K1_9ARCH)

HSP 1 Score: 56.2 bits (134), Expect = 1.700e-8
Identity = 27/41 (65.85%), Postives = 30/41 (73.17%), Query Frame = 1 
Query:    1 MDPRVSVAWCKRHEGP---VFAKTLQAKFV*AMNVPPDWNF 114
            MDPR+SVAWCKR E P   VFA+TL+ KF  AM  PPDW F
Sbjct:    1 MDPRISVAWCKRCEVPIDKVFARTLRDKFNWAMAAPPDWKF 41
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BLAST of mRNA_Ecto-sp13_S_contig8724.20192.1 vs. uniprot
Match: A0A7S0FJQ3_9STRA (Hypothetical protein n=1 Tax=Minutocellus polymorphus TaxID=265543 RepID=A0A7S0FJQ3_9STRA)

HSP 1 Score: 57.4 bits (137), Expect = 2.680e-8
Identity = 27/44 (61.36%), Postives = 33/44 (75.00%), Query Frame = 1 
Query:    1 MDPRVSVAWCKRHEGPV---FAKTLQAKFV*AMNVPPDWNFSYE 123
            MDPR+SVAWCKR+E P+   F+KTL+ KF  AM VPP W F+ E
Sbjct:   58 MDPRISVAWCKRNEVPIEKIFSKTLRDKFNWAMAVPPTWEFNGE 101
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BLAST of mRNA_Ecto-sp13_S_contig8724.20192.1 vs. uniprot
Match: A0A7S4VCD4_9STRA (Hypothetical protein n=2 Tax=Ditylum brightwellii TaxID=49249 RepID=A0A7S4VCD4_9STRA)

HSP 1 Score: 60.5 bits (145), Expect = 3.150e-8
Identity = 28/44 (63.64%), Postives = 34/44 (77.27%), Query Frame = 1 
Query:    1 MDPRVSVAWCKRHEGPV---FAKTLQAKFV*AMNVPPDWNFSYE 123
            MDPR+SVAWCKR+E P+   F+KTL+ KF  AM VPPDW F+ E
Sbjct:  684 MDPRISVAWCKRNEVPIEKIFSKTLRDKFNWAMAVPPDWEFNAE 727
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BLAST of mRNA_Ecto-sp13_S_contig8724.20192.1 vs. uniprot
Match: A0A7S1ZX42_TRICV (Hypothetical protein n=1 Tax=Trieres chinensis TaxID=1514140 RepID=A0A7S1ZX42_TRICV)

HSP 1 Score: 60.1 bits (144), Expect = 4.240e-8
Identity = 28/44 (63.64%), Postives = 34/44 (77.27%), Query Frame = 1 
Query:    1 MDPRVSVAWCKRHEGPV---FAKTLQAKFV*AMNVPPDWNFSYE 123
            MDPR+SVAWCKR+E P+   F+KTL+ KF  AM VPPDW F+ E
Sbjct:  584 MDPRISVAWCKRNEVPIEKIFSKTLRDKFNWAMAVPPDWEFNGE 627
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BLAST of mRNA_Ecto-sp13_S_contig8724.20192.1 vs. uniprot
Match: F0WN99_9STRA (DNA topoisomerase I n=1 Tax=Albugo laibachii Nc14 TaxID=890382 RepID=F0WN99_9STRA)

HSP 1 Score: 60.1 bits (144), Expect = 4.310e-8
Identity = 28/41 (68.29%), Postives = 32/41 (78.05%), Query Frame = 1 
Query:    1 MDPRVSVAWCKRHEGP---VFAKTLQAKFV*AMNVPPDWNF 114
            MDPR+SVAWCKR+E P   VFAKTL+ KFV AM+  PDW F
Sbjct:  708 MDPRISVAWCKRNEVPISAVFAKTLREKFVWAMDAAPDWKF 748
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BLAST of mRNA_Ecto-sp13_S_contig8724.20192.1 vs. uniprot
Match: A0A4D9D5J1_9STRA (TOPEUc domain-containing protein n=2 Tax=Monodopsidaceae TaxID=425072 RepID=A0A4D9D5J1_9STRA)

HSP 1 Score: 59.7 bits (143), Expect = 5.950e-8
Identity = 28/41 (68.29%), Postives = 31/41 (75.61%), Query Frame = 1 
Query:    1 MDPRVSVAWCKRHEGPV---FAKTLQAKFV*AMNVPPDWNF 114
            MDPR+SVAWCKR E P+   FAKTL+ KFV AM VPP W F
Sbjct:  823 MDPRISVAWCKRVEVPIERIFAKTLRDKFVWAMGVPPSWRF 863
The following BLAST results are available for this feature:
BLAST of mRNA_Ecto-sp13_S_contig8724.20192.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
D7G047_ECTSI3.430e-3385.25Topo_C_assoc domain-containing protein n=1 Tax=Ect... [more]
D7G042_ECTSI3.210e-2050.85TOPEUc domain-containing protein n=1 Tax=Ectocarpu... [more]
A0A6H5K298_9PHAE2.290e-1378.00Uncharacterized protein n=1 Tax=Ectocarpus sp. CCA... [more]
A0A836C9X0_9STRA1.110e-1260.34DNA topoisomerase I n=1 Tax=Tribonema minus TaxID=... [more]
A0A482T3K1_9ARCH1.700e-865.85Topo_C_assoc domain-containing protein n=1 Tax=arc... [more]
A0A7S0FJQ3_9STRA2.680e-861.36Hypothetical protein n=1 Tax=Minutocellus polymorp... [more]
A0A7S4VCD4_9STRA3.150e-863.64Hypothetical protein n=2 Tax=Ditylum brightwellii ... [more]
A0A7S1ZX42_TRICV4.240e-863.64Hypothetical protein n=1 Tax=Trieres chinensis Tax... [more]
F0WN99_9STRA4.310e-868.29DNA topoisomerase I n=1 Tax=Albugo laibachii Nc14 ... [more]
A0A4D9D5J1_9STRA5.950e-868.29TOPEUc domain-containing protein n=2 Tax=Monodopsi... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
Ecto-sp13_S_contig8724contigEcto-sp13_S_contig8724:4979..5911 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Ectocarpus species13 EcNAP12_S_4_19m OGS1.02022-07-08
Properties
Property NameValue
Taxonomic scopeEukaryota
Stop0
Start1
Seed ortholog score79.0
Seed ortholog evalue1.3e-12
Seed eggNOG ortholog2880.D7G047
Preferred nameTOP1
Model size357
KEGG koko:K03163
Hectar predicted targeting categoryother localisation
GOsGO:0000166,GO:0000228,GO:0000932,GO:0000976,GO:0000977,GO:0000978,GO:0000987,GO:0001012,GO:0001067,GO:0001650,GO:0003674,GO:0003676,GO:0003677,GO:0003682,GO:0003690,GO:0003697,GO:0003824,GO:0003916,GO:0003917,GO:0004672,GO:0004674,GO:0005488,GO:0005515,GO:0005524,GO:0005575,GO:0005622,GO:0005623,GO:0005634,GO:0005654,GO:0005657,GO:0005694,GO:0005730,GO:0005737,GO:0006139,GO:0006259,GO:0006260,GO:0006265,GO:0006325,GO:0006338,GO:0006464,GO:0006468,GO:0006725,GO:0006793,GO:0006796,GO:0006807,GO:0006996,GO:0007059,GO:0007623,GO:0008144,GO:0008150,GO:0008152,GO:0008219,GO:0009058,GO:0009059,GO:0009330,GO:0009987,GO:0010468,GO:0012501,GO:0016043,GO:0016301,GO:0016310,GO:0016740,GO:0016772,GO:0016773,GO:0016853,GO:0017076,GO:0018105,GO:0018193,GO:0018209,GO:0019222,GO:0019538,GO:0019904,GO:0030554,GO:0031298,GO:0031974,GO:0031981,GO:0032501,GO:0032553,GO:0032555,GO:0032559,GO:0032922,GO:0032991,GO:0032993,GO:0034641,GO:0034645,GO:0035639,GO:0035770,GO:0036094,GO:0036211,GO:0036464,GO:0042221,GO:0042493,GO:0043167,GO:0043168,GO:0043170,GO:0043226,GO:0043227,GO:0043228,GO:0043229,GO:0043231,GO:0043232,GO:0043233,GO:0043412,GO:0043565,GO:0043596,GO:0044212,GO:0044237,GO:0044238,GO:0044249,GO:0044260,GO:0044267,GO:0044422,GO:0044424,GO:0044427,GO:0044428,GO:0044444,GO:0044446,GO:0044452,GO:0044454,GO:0044464,GO:0046483,GO:0048511,GO:0050789,GO:0050896,GO:0051276,GO:0060255,GO:0065007,GO:0070013,GO:0071103,GO:0071704,GO:0071840,GO:0090304,GO:0097100,GO:0097159,GO:0097367,GO:0140096,GO:0140097,GO:1901265,GO:1901360,GO:1901363,GO:1901564,GO:1901576,GO:1990837,GO:1990904
Exons2
EggNOG free text desc.DNA topoisomerase type I activity
EggNOG OGsCOG3569@1,KOG0981@2759
Ec32 ortholog descriptionDNA topoisomerase I, catalytic core, alpha/beta subdomain
Ec32 orthologEc-12_007190.1
EC5.99.1.2
Cds size270
COG Functional cat.L
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko01000,ko03032
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1681464145.3916693-CDS-Ecto-sp13_S_contig8724:4978..52481681464145.3916693-CDS-Ecto-sp13_S_contig8724:4978..5248Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig8724 4979..5248 -


The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1681464145.4063706-UTR-Ecto-sp13_S_contig8724:5248..52871681464145.4063706-UTR-Ecto-sp13_S_contig8724:5248..5287Ectocarpus species13 EcNAP12_S_4_19mUTREcto-sp13_S_contig8724 5249..5287 -
1681464145.4179683-UTR-Ecto-sp13_S_contig8724:5863..59111681464145.4179683-UTR-Ecto-sp13_S_contig8724:5863..5911Ectocarpus species13 EcNAP12_S_4_19mUTREcto-sp13_S_contig8724 5864..5911 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_Ecto-sp13_S_contig8724.20192.1prot_Ecto-sp13_S_contig8724.20192.1Ectocarpus species13 EcNAP12_S_4_19mpolypeptideEcto-sp13_S_contig8724 4979..5248 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_Ecto-sp13_S_contig8724.20192.1

>prot_Ecto-sp13_S_contig8724.20192.1 ID=prot_Ecto-sp13_S_contig8724.20192.1|Name=mRNA_Ecto-sp13_S_contig8724.20192.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=polypeptide|length=90bp
MNVPPDWNFSYETLYNSKQSLLDVPRPPGGGKKKADAAAATTTAKTPPKT
AAAAAAAKKPAAAEGSACASAASPKNPEVKGKGKAKNVED
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mRNA from alignment at Ecto-sp13_S_contig8724:4979..5911-

Legend: UTRCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_Ecto-sp13_S_contig8724.20192.1 ID=mRNA_Ecto-sp13_S_contig8724.20192.1|Name=mRNA_Ecto-sp13_S_contig8724.20192.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=mRNA|length=933bp|location=Sequence derived from alignment at Ecto-sp13_S_contig8724:4979..5911- (Ectocarpus species13 EcNAP12_S_4_19m)
ATGGACCCTCGCGTGAGCGTGGCATGGTGCAAGCGTCACGAGGGCCCTGT
GGAAAAGGCAAGTGGGGGTTACTTTCGCCTCGAGTAAGCAAGCCTTCCCC
CATGCTGGCGTGCCGTGCTCGCACAGGTTGTACTGCTGTAGACGTCCGGT
TGGAAGCTGTTCTATTGGATGTGATCAAGGAGGAAACTCTCCCGGCCTGT
TGCTGGCCGTTGTGGCTGTGTTTTGGTTGTTGTTGTGGTCCTTTTCGAAC
TACCATTATCTCGTCAAGAACGGCCACGCAATGATGTGGTCGTGGCGTGT
ACCTCATCCTGCCACTACCGGAGGTTACGGCAAAGAGCGAGTGTGGATAG
TGTAGATACCTGCTATGCTAGGCACACGAGAACGAGAGAGACTGGTGGGA
TGCCTACCACGGCGTATGTTTTCGTGCCTGACGCACCGCTTTTCCTTGTT
TCAAACATGCCTCCGTCACCTTGCTTCGATTGTCAGAGTTGCGGCAGACG
CGAAGGGTTGCCCCGTGTTGGCTGTCGGGCGCTCCGTTGCCACCCTCCCC
CCCTCGTTCGGGAAGTCGGATATCCCTCACCCTCTCCCCTGTCCATTGCT
ATCTGATTCGAATGCATGTAACAGGTGTTCGCGAAGACTCTGCAGGCCAA
GTTCGTGTGAGCGATGAACGTGCCCCCCGACTGGAACTTCTCGTACGAGA
CTCTCTACAACTCCAAACAGAGCCTGTTGGACGTTCCTCGCCCCCCGGGC
GGCGGCAAGAAGAAGGCCGATGCCGCTGCTGCTACCACCACCGCTAAGAC
ACCCCCCAAGACTGCTGCTGCCGCTGCCGCTGCGAAGAAACCGGCCGCTG
CTGAAGGGTCTGCGTGTGCGTCTGCAGCATCGCCGAAAAATCCGGAGGTG
AAGGGTAAGGGTAAGGCTAAGAATGTCGAGGAT
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Coding sequence (CDS) from alignment at Ecto-sp13_S_contig8724:4979..5911-

>mRNA_Ecto-sp13_S_contig8724.20192.1 ID=mRNA_Ecto-sp13_S_contig8724.20192.1|Name=mRNA_Ecto-sp13_S_contig8724.20192.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=CDS|length=270bp|location=Sequence derived from alignment at Ecto-sp13_S_contig8724:4979..5911- (Ectocarpus species13 EcNAP12_S_4_19m)
ATGAACGTGCCCCCCGACTGGAACTTCTCGTACGAGACTCTCTACAACTC
CAAACAGAGCCTGTTGGACGTTCCTCGCCCCCCGGGCGGCGGCAAGAAGA
AGGCCGATGCCGCTGCTGCTACCACCACCGCTAAGACACCCCCCAAGACT
GCTGCTGCCGCTGCCGCTGCGAAGAAACCGGCCGCTGCTGAAGGGTCTGC
GTGTGCGTCTGCAGCATCGCCGAAAAATCCGGAGGTGAAGGGTAAGGGTA
AGGCTAAGAATGTCGAGGAT
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