mRNA_Ecto-sp13_S_contig8442.19823.1 (mRNA) Ectocarpus species13 EcNAP12_S_4_19m

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_Ecto-sp13_S_contig8442.19823.1
Unique NamemRNA_Ecto-sp13_S_contig8442.19823.1
TypemRNA
OrganismEctocarpus species13 EcNAP12_S_4_19m (Ectocarpus species13 EcNAP12_S_4_19m)
Homology
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BLAST of mRNA_Ecto-sp13_S_contig8442.19823.1 vs. uniprot
Match: D8LGJ4_ECTSI (Phospholipid-transporting ATPase n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LGJ4_ECTSI)

HSP 1 Score: 194 bits (493), Expect = 1.890e-55
Identity = 96/101 (95.05%), Postives = 100/101 (99.01%), Query Frame = 1 
Query:    1 GQMLGVNFVDRSPGKVELDVTGKGRLSYSLILTIPFDSTRKRMSVVVRAPDGSYVLYCKGADNIIMDRARGYMGSDKETVASHLGVFSNDGLRTLLLAKKD 303
            G+ LGVNFVDRSPGKVELDVTGKGRLSY+LILTIPFDSTRKRMSVVVRAPDGSYVLYCKGADNIIMDR+RGYMGSDKETVASHLGVFSNDGLRTLLLAKK+
Sbjct:  541 GKTLGVNFVDRSPGKVELDVTGKGRLSYNLILTIPFDSTRKRMSVVVRAPDGSYVLYCKGADNIIMDRSRGYMGSDKETVASHLGVFSNDGLRTLLLAKKE 641
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BLAST of mRNA_Ecto-sp13_S_contig8442.19823.1 vs. uniprot
Match: A0A6H5JDV8_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JDV8_9PHAE)

HSP 1 Score: 117 bits (292), Expect = 3.650e-31
Identity = 57/59 (96.61%), Postives = 58/59 (98.31%), Query Frame = 1 
Query:    1 GQMLGVNFVDRSPGKVELDVTGKGRLSYSLILTIPFDSTRKRMSVVVRAPDGSYVLYCK 177
            G+ LGVNFVDRSPGKVELDVTGKGRLSYSLILTIPFDSTRKRMSVVVRAPDGSYVLYCK
Sbjct:  117 GKTLGVNFVDRSPGKVELDVTGKGRLSYSLILTIPFDSTRKRMSVVVRAPDGSYVLYCK 175
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BLAST of mRNA_Ecto-sp13_S_contig8442.19823.1 vs. uniprot
Match: A0A836CHZ9_9STRA (Phospholipid-transporting ATPase n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A836CHZ9_9STRA)

HSP 1 Score: 100 bits (249), Expect = 1.530e-22
Identity = 52/99 (52.53%), Postives = 69/99 (69.70%), Query Frame = 1 
Query:    4 QMLGVNFVDRSPGKVELDVTGKGRLSYSLILTIPFDSTRKRMSVVVRAPDGSYVLYCKGADNIIMDRARGYMGSDKETVASHLGVFSNDGLRTLLLAKK 300
            + LGV FVDR PG V + V G+ +  + L+ T+PFDSTRKRMSV+VR   G    Y KGADN+I++RARG   ++   +A  LG F+ DGLRTLLLA++
Sbjct:  645 RQLGVEFVDREPGTVVVRVAGREQ-RFELLATLPFDSTRKRMSVLVRDASGRVTAYTKGADNVILERARG--AANARLIAQQLGAFAEDGLRTLLLARR 740
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BLAST of mRNA_Ecto-sp13_S_contig8442.19823.1 vs. uniprot
Match: A0A7S2CN35_9STRA (Hypothetical protein (Fragment) n=1 Tax=Dictyocha speculum TaxID=35687 RepID=A0A7S2CN35_9STRA)

HSP 1 Score: 96.3 bits (238), Expect = 7.970e-22
Identity = 49/101 (48.51%), Postives = 69/101 (68.32%), Query Frame = 1 
Query:    1 GQMLGVNFVDRSPGKVELDVTGKGRLSYSLILTIPFDSTRKRMSVVVRAPDGSYVLYCKGADNIIMDRARGY-MGSDKETVASHLGVFSNDGLRTLLLAKK 300
            G  LG  F   S  +V + + GK + +Y L+  IPFDS RKRMSV++R PDGS+V +CKGADNII DR+  Y +   ++ +  HL +F+N+GLRTL+L+ K
Sbjct:   79 GIALGYEFAGSSTTEVRVKIAGKQQ-AYQLLALIPFDSKRKRMSVLLRLPDGSFVFFCKGADNIIFDRSSDYSLVGSQDLLNEHLEIFANEGLRTLVLSMK 178
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BLAST of mRNA_Ecto-sp13_S_contig8442.19823.1 vs. uniprot
Match: W7TGI1_9STRA (Phospholipid-transporting ATPase n=2 Tax=Monodopsidaceae TaxID=425072 RepID=W7TGI1_9STRA)

HSP 1 Score: 97.1 bits (240), Expect = 2.500e-21
Identity = 50/103 (48.54%), Postives = 68/103 (66.02%), Query Frame = 1 
Query:   10 LGVNFVDRSPGKVELDVTGKGR-----LSYSLILTIPFDSTRKRMSVVVRAPDGSYVLYCKGADNIIMDRARGYMGSDKETVASHLGVFSNDGLRTLLLAKKD 303
            LG  F  R PG+V L V G  +     L++ L+ TI F STRKRMSV+V+ PDG  +L  KGADNI+  RA+ +  +D + V +HL +FS DGLRTL+LA ++
Sbjct:  529 LGFRFTGRGPGEVRLKVGGDDKAGGEELTFQLLCTIAFTSTRKRMSVIVKTPDGKVLLLTKGADNIVGGRAKEFHSTDSDAVDAHLRLFSEDGLRTLMLAVRE 631
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BLAST of mRNA_Ecto-sp13_S_contig8442.19823.1 vs. uniprot
Match: A0A7S3NH53_9STRA (Phospholipid-transporting ATPase n=1 Tax=Aureoumbra lagunensis TaxID=44058 RepID=A0A7S3NH53_9STRA)

HSP 1 Score: 92.0 bits (227), Expect = 1.420e-19
Identity = 48/103 (46.60%), Postives = 65/103 (63.11%), Query Frame = 1 
Query:   10 LGVNFVDRSPGKVELDVTGKG-----RLSYSLILTIPFDSTRKRMSVVVRAPDGSYVLYCKGADNIIMDRARGYMGSDKETVASHLGVFSNDGLRTLLLAKKD 303
            LG++F+DR   KV +     G      L Y ++ TIPFDSTRKRMSVV R PDG+  + CKGAD I+     G    D+ ++ +HL  F+ DGLRTL+LA++D
Sbjct:  413 LGLSFIDRKAEKVIIQAAATGGDGGTNLEYEILATIPFDSTRKRMSVVCRLPDGTIRIMCKGADTIVFGLLAG--SEDQASLNTHLDAFARDGLRTLVLAQRD 513
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BLAST of mRNA_Ecto-sp13_S_contig8442.19823.1 vs. uniprot
Match: A0A0L0DSL9_THETB (Phospholipid-transporting ATPase n=1 Tax=Thecamonas trahens ATCC 50062 TaxID=461836 RepID=A0A0L0DSL9_THETB)

HSP 1 Score: 91.7 bits (226), Expect = 1.930e-19
Identity = 47/98 (47.96%), Postives = 65/98 (66.33%), Query Frame = 1 
Query:   10 LGVNFVDRSPGKVELDVTGKGRLSYSLILTIPFDSTRKRMSVVVRAPDGSYVLYCKGADNIIMDRARGYMGSDKETVASHLGVFSNDGLRTLLLAKKD 303
            LG  F  RS  +V ++V GK  + Y+L+  + F+STRKRMSV+VR PDG+  LYCKGAD++I +R  G      +  + HL  F+ DGLRTL+LA +D
Sbjct:  203 LGYKFASRSASEVVIEVFGKPAV-YTLLHVLEFNSTRKRMSVIVRTPDGNIKLYCKGADSVIYERLAGGQKELMDVTSQHLEEFARDGLRTLVLAVRD 299
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BLAST of mRNA_Ecto-sp13_S_contig8442.19823.1 vs. uniprot
Match: A0A7S2CW43_9STRA (Phospholipid-transporting ATPase n=1 Tax=Dictyocha speculum TaxID=35687 RepID=A0A7S2CW43_9STRA)

HSP 1 Score: 89.4 bits (220), Expect = 1.220e-18
Identity = 47/101 (46.53%), Postives = 64/101 (63.37%), Query Frame = 1 
Query:    1 GQMLGVNFVDRSPGKVELDVTGKGRLSYSLILTIPFDSTRKRMSVVVRAPDGSYVLYCKGADNIIMDRARGY-MGSDKETVASHLGVFSNDGLRTLLLAKK 300
            G+ LG  +   S   + +D + K R++Y L+  IPF S RKRMSV++    G  +LYCKGADNI+ DRA  Y +   KET+  HL + S+ GLRTL+LA K
Sbjct:   74 GKKLGYTYAGTSTDTIFVDRSDK-RVNYKLLALIPFSSLRKRMSVLIETESGDIILYCKGADNIMFDRASDYKLAGSKETLNQHLEICSSQGLRTLVLAMK 173
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BLAST of mRNA_Ecto-sp13_S_contig8442.19823.1 vs. uniprot
Match: A0A7S2SIW9_9STRA (Phospholipid-transporting ATPase (Fragment) n=1 Tax=Rhizochromulina marina TaxID=1034831 RepID=A0A7S2SIW9_9STRA)

HSP 1 Score: 89.4 bits (220), Expect = 1.240e-18
Identity = 45/102 (44.12%), Postives = 67/102 (65.69%), Query Frame = 1 
Query:    1 GQMLGVNFVDRSPGKVELDVTGKGRLSYSLILTIPFDSTRKRMSVVVRAPDGSYVLYCKGADNIIMDRARG--YMGSDKETVASHLGVFSNDGLRTLLLAKK 300
            G  LG  +V  S   + ++  G   L + L+  IPFDSTRKRMS++V +P+G Y+ +CKGADN+I DRA     +  D++ + +HL +F+ +GLRTL+LA K
Sbjct:  374 GIQLGFTYVGSSSDSIRVEHMG-ATLEFKLLALIPFDSTRKRMSLLVISPEGEYIFFCKGADNVIFDRASTDYSLVGDRKILENHLEIFATEGLRTLVLAMK 474
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BLAST of mRNA_Ecto-sp13_S_contig8442.19823.1 vs. uniprot
Match: W7TQX5_9STRA (Phospholipid-transporting ATPase n=2 Tax=Monodopsidaceae TaxID=425072 RepID=W7TQX5_9STRA)

HSP 1 Score: 89.0 bits (219), Expect = 1.730e-18
Identity = 44/99 (44.44%), Postives = 69/99 (69.70%), Query Frame = 1 
Query:   10 LGVNFVDRSPGKVELDVTGKGRLSYSLILTIPFDSTRKRMSVVVRAP-DGSYVLYCKGADNIIMDRARGYMGSDKETVASHLGVFSNDGLRTLLLAKKD 303
            LG  F  RS  +  ++     RL+Y ++ T+PF STRKRMSV+VR P +G  VL  KGAD+++ +RA  ++G+ +E + +HL  F++DGLRTL+LA+++
Sbjct:  688 LGWRFDGRSSTEALVEAPLGRRLTYQVLATLPFTSTRKRMSVIVRRPGEGKVVLLMKGADSVVFERASNFLGAAREVLDAHLSEFASDGLRTLVLARRE 786
The following BLAST results are available for this feature:
BLAST of mRNA_Ecto-sp13_S_contig8442.19823.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
D8LGJ4_ECTSI1.890e-5595.05Phospholipid-transporting ATPase n=1 Tax=Ectocarpu... [more]
A0A6H5JDV8_9PHAE3.650e-3196.61Uncharacterized protein n=1 Tax=Ectocarpus sp. CCA... [more]
A0A836CHZ9_9STRA1.530e-2252.53Phospholipid-transporting ATPase n=1 Tax=Tribonema... [more]
A0A7S2CN35_9STRA7.970e-2248.51Hypothetical protein (Fragment) n=1 Tax=Dictyocha ... [more]
W7TGI1_9STRA2.500e-2148.54Phospholipid-transporting ATPase n=2 Tax=Monodopsi... [more]
A0A7S3NH53_9STRA1.420e-1946.60Phospholipid-transporting ATPase n=1 Tax=Aureoumbr... [more]
A0A0L0DSL9_THETB1.930e-1947.96Phospholipid-transporting ATPase n=1 Tax=Thecamona... [more]
A0A7S2CW43_9STRA1.220e-1846.53Phospholipid-transporting ATPase n=1 Tax=Dictyocha... [more]
A0A7S2SIW9_9STRA1.240e-1844.12Phospholipid-transporting ATPase (Fragment) n=1 Ta... [more]
W7TQX5_9STRA1.730e-1844.44Phospholipid-transporting ATPase n=2 Tax=Monodopsi... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
Ecto-sp13_S_contig8442contigEcto-sp13_S_contig8442:6169..6709 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Ectocarpus species13 EcNAP12_S_4_19m OGS1.02022-07-08
Properties
Property NameValue
Taxonomic scopeEukaryota
Stop0
Start1
Seed ortholog score192.6
Seed ortholog evalue8.8e-47
Seed eggNOG ortholog2880.D8LGJ4
Preferred nameDNF3
Model size305
KEGG rclassRC00002
KEGG koko:K01509,ko:K01530,ko:K14802
KEGG TC3.A.3.8
KEGG ReactionR00086
KEGG Pathwayko00230,ko04742,map00230,map04742
Hectar predicted targeting categoryother localisation
GOsGO:0000749,GO:0003674,GO:0003824,GO:0004012,GO:0005215,GO:0005319,GO:0005548,GO:0005575,GO:0005622,GO:0005623,GO:0005737,GO:0005783,GO:0005794,GO:0005798,GO:0005802,GO:0005886,GO:0005937,GO:0006810,GO:0006811,GO:0006820,GO:0006869,GO:0006886,GO:0006892,GO:0006900,GO:0006996,GO:0008104,GO:0008150,GO:0009987,GO:0010033,GO:0010876,GO:0012505,GO:0015031,GO:0015711,GO:0015748,GO:0015833,GO:0015914,GO:0016020,GO:0016021,GO:0016043,GO:0016050,GO:0016192,GO:0016197,GO:0016462,GO:0016787,GO:0016817,GO:0016818,GO:0016887,GO:0017111,GO:0019236,GO:0030133,GO:0030135,GO:0030136,GO:0030140,GO:0030427,GO:0031136,GO:0031137,GO:0031139,GO:0031224,GO:0031410,GO:0031982,GO:0031984,GO:0032456,GO:0033036,GO:0034204,GO:0034613,GO:0042221,GO:0042623,GO:0042886,GO:0042995,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0043332,GO:0043492,GO:0043900,GO:0043902,GO:0044422,GO:0044424,GO:0044425,GO:0044431,GO:0044444,GO:0044446,GO:0044459,GO:0044463,GO:0044464,GO:0045184,GO:0045332,GO:0046907,GO:0046999,GO:0048193,GO:0048194,GO:0048518,GO:0048522,GO:0050789,GO:0050794,GO:0050896,GO:0051179,GO:0051234,GO:0051286,GO:0051641,GO:0051649,GO:0051716,GO:0061024,GO:0065007,GO:0065008,GO:0070727,GO:0070867,GO:0070887,GO:0071310,GO:0071444,GO:0071702,GO:0071705,GO:0071840,GO:0071944,GO:0097035,GO:0097708,GO:0098791,GO:0120025,GO:0120038,GO:2000241,GO:2000243
Exons2
EggNOG free text desc.phospholipid-translocating ATPase activity
EggNOG OGsCOG0474@1,KOG0206@2759
EC3.6.1.3,3.6.3.1
Cds size297
COG Functional cat.P
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko01000,ko03009
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1681464113.9623811-CDS-Ecto-sp13_S_contig8442:6168..62941681464113.9623811-CDS-Ecto-sp13_S_contig8442:6168..6294Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig8442 6169..6294 -
1681464113.9779134-CDS-Ecto-sp13_S_contig8442:6532..67031681464113.9779134-CDS-Ecto-sp13_S_contig8442:6532..6703Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig8442 6533..6703 -


The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1681464113.9950726-UTR-Ecto-sp13_S_contig8442:6703..67091681464113.9950726-UTR-Ecto-sp13_S_contig8442:6703..6709Ectocarpus species13 EcNAP12_S_4_19mUTREcto-sp13_S_contig8442 6704..6709 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_Ecto-sp13_S_contig8442.19823.1prot_Ecto-sp13_S_contig8442.19823.1Ectocarpus species13 EcNAP12_S_4_19mpolypeptideEcto-sp13_S_contig8442 6169..6703 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_Ecto-sp13_S_contig8442.19823.1

>prot_Ecto-sp13_S_contig8442.19823.1 ID=prot_Ecto-sp13_S_contig8442.19823.1|Name=mRNA_Ecto-sp13_S_contig8442.19823.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=polypeptide|length=99bp
MLGVNFVDRSPGKVELDVTGKGRLSYSLILTIPFDSTRKRMSVVVRAPDG
SYVLYCKGADNIIMDRARGYMGSDKETVASHLGVFSNDGLRTLLLAKKD
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mRNA from alignment at Ecto-sp13_S_contig8442:6169..6709-

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_Ecto-sp13_S_contig8442.19823.1 ID=mRNA_Ecto-sp13_S_contig8442.19823.1|Name=mRNA_Ecto-sp13_S_contig8442.19823.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=mRNA|length=541bp|location=Sequence derived from alignment at Ecto-sp13_S_contig8442:6169..6709- (Ectocarpus species13 EcNAP12_S_4_19m)
GGGCAGATGCTGGGGGTGAATTTCGTGGACCGCTCGCCGGGGAAGGTGGA
GCTGGACGTGACCGGAAAGGGTCGACTGTCGTACAGCCTCATCCTCACAA
TCCCCTTCGACTCGACGAGGAAGCGGATGTCGGTCGTTGTCCGTGCTCCC
GACGGCTCCTACGTCTTGTACTGCAAGGTATGTAAAACGTTTGGGGTCGG
TTGTGCGTGTGTGAGTGTGTGTGTCGCGTCGGGGTAGTTTTTGTGAAATT
TCGCAACCTCCTCGCACATAACCTTCTGATTGCTAAGCCGGGAGTTCCAA
CACCAGCAGCGGAGTGCGATACCCTCCCCCCCCTTCCCTGCGTTGGGACA
TCATGATGCACTAACATATGAGCACACCCTCCCGCCCGTTTAATCCCTTT
TTGTGTACGGTGAAGGGAGCGGACAACATCATCATGGACCGTGCAAGGGG
ATACATGGGGTCTGACAAGGAAACCGTGGCTTCGCATCTTGGTGTGTTTT
CCAACGACGGTCTTCGCACCCTGCTGCTGGCCAAGAAGGAC
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Coding sequence (CDS) from alignment at Ecto-sp13_S_contig8442:6169..6709-

>mRNA_Ecto-sp13_S_contig8442.19823.1 ID=mRNA_Ecto-sp13_S_contig8442.19823.1|Name=mRNA_Ecto-sp13_S_contig8442.19823.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=CDS|length=297bp|location=Sequence derived from alignment at Ecto-sp13_S_contig8442:6169..6709- (Ectocarpus species13 EcNAP12_S_4_19m)
ATGCTGGGGGTGAATTTCGTGGACCGCTCGCCGGGGAAGGTGGAGCTGGA
CGTGACCGGAAAGGGTCGACTGTCGTACAGCCTCATCCTCACAATCCCCT
TCGACTCGACGAGGAAGCGGATGTCGGTCGTTGTCCGTGCTCCCGACGGC
TCCTACGTCTTGTACTGCAAGGGAGCGGACAACATCATCATGGACCGTGC
AAGGGGATACATGGGGTCTGACAAGGAAACCGTGGCTTCGCATCTTGGTG
TGTTTTCCAACGACGGTCTTCGCACCCTGCTGCTGGCCAAGAAGGAC
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